BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00015548
         (1052 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AER38245.1| PME4 [Gossypium barbadense]                            319   6e-85
gb|AAQ21124.1| pectinesterase [Fragaria x ananassa]                   313   3e-83
sp|Q43062.1|PME_PRUPE RecName: Full=Pectinesterase/pectinesteras...   313   5e-83
emb|CBI29628.3| unnamed protein product [Vitis vinifera]              310   3e-82
emb|CAN60612.1| hypothetical protein VITISV_003251 [Vitis vinifera]   310   3e-82

>gb|AER38245.1| PME4 [Gossypium barbadense]
          Length = 525

 Score =  319 bits (818), Expect = 6e-85
 Identities = 178/334 (53%), Positives = 216/334 (64%), Gaps = 5/334 (1%)
 Frame = -1

Query: 989 VLVILLCTISCFGDDL--MYQPDCLNVPTTEFLSSLKTTIDVIQQVTSTVAAFSRGFLDF 816
           ++ + LCT S          + +CL VP TEF+ SLKTTID I++ TS V+ F   F DF
Sbjct: 12  LVALCLCTNSNASSSTNEFLETECLKVPATEFIGSLKTTIDAIRKATSVVSQFGGFFHDF 71

Query: 815 RLSNAIADCXXXXXXXXXXXXXXXXXTQNPHNSKDNSTGNLSADLRTWLSAALGNQDTCV 636
           RLSNAI+DC                 +QNP N+KDNSTG+LS+DLRTWLSAA+ NQ TC+
Sbjct: 72  RLSNAISDCLDLLDSSADELSWTMSASQNP-NAKDNSTGDLSSDLRTWLSAAMVNQQTCI 130

Query: 635 EGFDGTKGFVKSLVSSSLDQITSLVRAILANVH---DKKSXXXXXXXXXXXXXGWMHKRK 465
           +GF+GT   VK++VS SL+QITSLVR +L  VH   + KS             G      
Sbjct: 131 DGFEGTNSMVKTVVSGSLNQITSLVRNLLIMVHPGPNSKSNGTRNGSQKGGGGGGHP--- 187

Query: 464 LIGGEKFPEWVKSSDRKKLLQXXXXXXXXXXXXXXXXNFTTLSDAIKTVPDYSTRRTVIY 285
             G  +FP W K  DR +LLQ                NFT + DA++T PD S  R VIY
Sbjct: 188 --GQSRFPVWFKREDR-RLLQINGVTANVVVAADGSGNFTRIMDAVETAPDKSMNRYVIY 244

Query: 284 IKKGVYNEYVEISKKKWNVMLVGDGMDVTVISGNHSFIGGWTTYRSATFGVKGRGFIARD 105
           IKKG+Y E VEI KKKWN++++GDGMDVTVISGN SFI GWTT RSATF V GRGFIARD
Sbjct: 245 IKKGLYKENVEIKKKKWNLVMIGDGMDVTVISGNRSFIDGWTTLRSATFAVSGRGFIARD 304

Query: 104 MTFENTAGPENHQAVAFRSDSDLSVLYRCAMRGY 3
           +TFENTAGP+ HQAVA RSDSDLSV +RCA++GY
Sbjct: 305 ITFENTAGPQKHQAVALRSDSDLSVFFRCAIKGY 338


>gb|AAQ21124.1| pectinesterase [Fragaria x ananassa]
          Length = 514

 Score =  313 bits (803), Expect = 3e-83
 Identities = 176/341 (51%), Positives = 207/341 (60%)
 Frame = -1

Query: 1025 HRMATPAKKNVTVLVILLCTISCFGDDLMYQPDCLNVPTTEFLSSLKTTIDVIQQVTSTV 846
            H M TP    V V +       C       Q +CL VP +EF  S+  TI+V+QQV S +
Sbjct: 5    HLMWTPRFLLVLVFLSFPYYAQCGSISNTIQSECLKVPNSEFGGSIINTINVLQQVVSIL 64

Query: 845  AAFSRGFLDFRLSNAIADCXXXXXXXXXXXXXXXXXTQNPHNSKDNSTGNLSADLRTWLS 666
            +  ++GF DFRLSNA+ DC                 TQN  N K NSTGNLS+DLRTWLS
Sbjct: 65   SDVAKGFGDFRLSNAVDDCLELMDDSTDQLSWTLSATQNK-NGKHNSTGNLSSDLRTWLS 123

Query: 665  AALGNQDTCVEGFDGTKGFVKSLVSSSLDQITSLVRAILANVHDKKSXXXXXXXXXXXXX 486
            A L NQDTC EG DGT   VKSLVS SL+QITSLV  +L  VH                 
Sbjct: 124  ATLVNQDTCNEGLDGTNSIVKSLVSGSLNQITSLVLELLGQVHPTSD------------- 170

Query: 485  GWMHKRKLIGGEKFPEWVKSSDRKKLLQXXXXXXXXXXXXXXXXNFTTLSDAIKTVPDYS 306
                + +   G+  P W K+ DRK LLQ                NFT ++ AI + PDYS
Sbjct: 171  ----QHESSNGQT-PAWFKAEDRK-LLQANGVPVDVVVAQDGTGNFTNITAAILSAPDYS 224

Query: 305  TRRTVIYIKKGVYNEYVEISKKKWNVMLVGDGMDVTVISGNHSFIGGWTTYRSATFGVKG 126
             +R VIY+KKG+Y EYVEI KKKWN+M++GDGMD TVISGNH+F+ GWTT+RSATF V G
Sbjct: 225  LKRYVIYVKKGLYKEYVEIKKKKWNIMMIGDGMDATVISGNHNFVDGWTTFRSATFAVSG 284

Query: 125  RGFIARDMTFENTAGPENHQAVAFRSDSDLSVLYRCAMRGY 3
            RGFIARD+TFENTAGPE H AVA RSDSDLS  YRC  RGY
Sbjct: 285  RGFIARDITFENTAGPEKHMAVALRSDSDLSAFYRCEFRGY 325


>sp|Q43062.1|PME_PRUPE RecName: Full=Pectinesterase/pectinesterase inhibitor PPE8B;
            Includes: RecName: Full=Pectinesterase inhibitor PPE8B;
            AltName: Full=Pectin methylesterase inhibitor PPE8B;
            Includes: RecName: Full=Pectinesterase PPE8B; Short=PE
            PPE8B; AltName: Full=Pectin methylesterase PPE8B; Flags:
            Precursor gi|1213629|emb|CAA65237.1| pectinesterase
            [Prunus persica]
          Length = 522

 Score =  313 bits (802), Expect = 5e-83
 Identities = 176/346 (50%), Positives = 211/346 (60%), Gaps = 10/346 (2%)
 Frame = -1

Query: 1010 PAKKNVTVLVILLCTI---------SC-FGDDLMYQPDCLNVPTTEFLSSLKTTIDVIQQ 861
            PA K + +L++LLC           SC   DDL  Q  CL V  TEF  SLK TID +QQ
Sbjct: 13   PAGKQL-LLLVLLCAFFSSSFIPFASCSITDDL--QTQCLKVSATEFAGSLKDTIDAVQQ 69

Query: 860  VTSTVAAFSRGFLDFRLSNAIADCXXXXXXXXXXXXXXXXXTQNPHNSKDNSTGNLSADL 681
            V S ++ F+  F DFRL+NAI+DC                 +QN    K+NSTG LS+DL
Sbjct: 70   VASILSQFANAFGDFRLANAISDCLDLLDFSADELNWSLSASQN-QKGKNNSTGKLSSDL 128

Query: 680  RTWLSAALGNQDTCVEGFDGTKGFVKSLVSSSLDQITSLVRAILANVHDKKSXXXXXXXX 501
            RTWLSAAL NQDTC  GF+GT   V+ L+S+ L Q+TSLV+ +L  VH   +        
Sbjct: 129  RTWLSAALVNQDTCSNGFEGTNSIVQGLISAGLGQVTSLVQELLTQVHPNSNQQGPNG-- 186

Query: 500  XXXXXGWMHKRKLIGGEKFPEWVKSSDRKKLLQXXXXXXXXXXXXXXXXNFTTLSDAIKT 321
                             + P WVK+ DRK LLQ                NFT ++DA+  
Sbjct: 187  -----------------QIPSWVKTKDRK-LLQADGVSVDAIVAQDGTGNFTNVTDAVLA 228

Query: 320  VPDYSTRRTVIYIKKGVYNEYVEISKKKWNVMLVGDGMDVTVISGNHSFIGGWTTYRSAT 141
             PDYS RR VIYIK+G Y E VEI KKKWN+M++GDGMD T+ISGN SF+ GWTT+RSAT
Sbjct: 229  APDYSMRRYVIYIKRGTYKENVEIKKKKWNLMMIGDGMDATIISGNRSFVDGWTTFRSAT 288

Query: 140  FGVKGRGFIARDMTFENTAGPENHQAVAFRSDSDLSVLYRCAMRGY 3
            F V GRGFIARD+TFENTAGPE HQAVA RSDSDLSV YRC +RGY
Sbjct: 289  FAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFYRCNIRGY 334


>emb|CBI29628.3| unnamed protein product [Vitis vinifera]
          Length = 527

 Score =  310 bits (795), Expect = 3e-82
 Identities = 164/313 (52%), Positives = 199/313 (63%)
 Frame = -1

Query: 941 MYQPDCLNVPTTEFLSSLKTTIDVIQQVTSTVAAFSRGFLDFRLSNAIADCXXXXXXXXX 762
           + Q  CL VP++ F+SSLK+TIDV++   S V+ F++ F DFRLSNAI+DC         
Sbjct: 38  LLQSQCLAVPSSAFISSLKSTIDVLRGTMSVVSQFTKVFNDFRLSNAISDCLELLDFAAD 97

Query: 761 XXXXXXXXTQNPHNSKDNSTGNLSADLRTWLSAALGNQDTCVEGFDGTKGFVKSLVSSSL 582
                    QNP   KDN TG+L +DL+TWLS+   NQDTC+EGF GT G VK++V+ SL
Sbjct: 98  DLSWSLSAIQNP-KGKDNGTGDLGSDLKTWLSSTFTNQDTCIEGFVGTNGIVKTVVAESL 156

Query: 581 DQITSLVRAILANVHDKKSXXXXXXXXXXXXXGWMHKRKLIGGEKFPEWVKSSDRKKLLQ 402
            Q+ SLV ++L  VHD                      K +G   FP WV    RK LLQ
Sbjct: 157 SQVASLVHSLLTMVHDPAPKGKSNGGGGGV--------KHVGSGDFPSWVGKHSRK-LLQ 207

Query: 401 XXXXXXXXXXXXXXXXNFTTLSDAIKTVPDYSTRRTVIYIKKGVYNEYVEISKKKWNVML 222
                           N+TT+ DA++  PDYS    VIYIK+G+Y E VEI KKKWN+M+
Sbjct: 208 ASSVSPDVTVAADGTGNYTTVMDAVQAAPDYSQNHYVIYIKQGIYRENVEIKKKKWNLMM 267

Query: 221 VGDGMDVTVISGNHSFIGGWTTYRSATFGVKGRGFIARDMTFENTAGPENHQAVAFRSDS 42
           VGDGM  TVI+GN S+I GWTTY SATF VKG+GFIARDMTFENTAGPE HQAVA RSDS
Sbjct: 268 VGDGMGATVITGNRSYIDGWTTYASATFAVKGKGFIARDMTFENTAGPEKHQAVALRSDS 327

Query: 41  DLSVLYRCAMRGY 3
           DLSV YRC+MRGY
Sbjct: 328 DLSVYYRCSMRGY 340


>emb|CAN60612.1| hypothetical protein VITISV_003251 [Vitis vinifera]
          Length = 534

 Score =  310 bits (795), Expect = 3e-82
 Identities = 164/313 (52%), Positives = 199/313 (63%)
 Frame = -1

Query: 941 MYQPDCLNVPTTEFLSSLKTTIDVIQQVTSTVAAFSRGFLDFRLSNAIADCXXXXXXXXX 762
           + Q  CL VP++ F+SSLK+TIDV++   S V+ F++ F DFRLSNAI+DC         
Sbjct: 42  LLQSQCLAVPSSAFISSLKSTIDVLRGTMSVVSQFTKVFNDFRLSNAISDCLELLDFAAD 101

Query: 761 XXXXXXXXTQNPHNSKDNSTGNLSADLRTWLSAALGNQDTCVEGFDGTKGFVKSLVSSSL 582
                    QNP   KDN TG+L +DL+TWLS+   NQDTC+EGF GT G VK++V+ SL
Sbjct: 102 DLSWSLSAIQNP-KGKDNGTGDLGSDLKTWLSSTFTNQDTCIEGFVGTNGIVKTVVAESL 160

Query: 581 DQITSLVRAILANVHDKKSXXXXXXXXXXXXXGWMHKRKLIGGEKFPEWVKSSDRKKLLQ 402
            Q+ SLV ++L  VHD                      K +G   FP WV    RK LLQ
Sbjct: 161 SQVASLVHSLLTMVHDPAPKGKSNGGGGGV--------KHVGSGDFPSWVGKHSRK-LLQ 211

Query: 401 XXXXXXXXXXXXXXXXNFTTLSDAIKTVPDYSTRRTVIYIKKGVYNEYVEISKKKWNVML 222
                           N+TT+ DA++  PDYS    VIYIK+G+Y E VEI KKKWN+M+
Sbjct: 212 ASSVSPDVTVAADGTGNYTTVMDAVQAAPDYSQNHYVIYIKQGIYRENVEIKKKKWNLMM 271

Query: 221 VGDGMDVTVISGNHSFIGGWTTYRSATFGVKGRGFIARDMTFENTAGPENHQAVAFRSDS 42
           VGDGM  TVI+GN S+I GWTTY SATF VKG+GFIARDMTFENTAGPE HQAVA RSDS
Sbjct: 272 VGDGMGATVITGNRSYIDGWTTYASATFAVKGKGFIARDMTFENTAGPEKHQAVALRSDS 331

Query: 41  DLSVLYRCAMRGY 3
           DLSV YRC+MRGY
Sbjct: 332 DLSVYYRCSMRGY 344