BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00014271
         (1524 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002273367.1| PREDICTED: probable glycerol kinase isoform ...   532   0.0  
emb|CAN76048.1| hypothetical protein VITISV_037711 [Vitis vinifera]   532   0.0  
ref|XP_004135863.1| PREDICTED: glycerol kinase-like [Cucumis sat...   542   0.0  
ref|XP_002327412.1| predicted protein [Populus trichocarpa] gi|2...   528   0.0  
ref|XP_003604353.1| Glycerol kinase [Medicago truncatula] gi|355...   516   0.0  

>ref|XP_002273367.1| PREDICTED: probable glycerol kinase isoform 1 [Vitis vinifera]
            gi|297743524|emb|CBI36391.3| unnamed protein product
            [Vitis vinifera]
          Length = 522

 Score =  532 bits (1370), Expect(2) = 0.0
 Identities = 261/336 (77%), Positives = 293/336 (87%), Gaps = 4/336 (1%)
 Frame = +3

Query: 528  LFGTIDSWLIWNMTGGTDGGLHVTDVSNAARTMLMNLKTLDWDKTALKELGIPSEILPKI 707
            LFGTID+WLIWNMTGG +GG+HVTDVSNA+RTMLMNLKTLDWDK  L  LGI +EILPKI
Sbjct: 169  LFGTIDTWLIWNMTGGLNGGVHVTDVSNASRTMLMNLKTLDWDKPTLDTLGISAEILPKI 228

Query: 708  VSNSEIIGNVTKGWPITGIPIAGCLGDQHAAMVGQACRKGEAKSTYGTGAFILLNTGDQM 887
            VSN+EIIG V KGWPI+G+PI+GCLGDQHAAM+GQACRKGEAKSTYGTGAFILLNTG+++
Sbjct: 229  VSNAEIIGTVAKGWPISGLPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEV 288

Query: 888  INSTHGLLSTVAFKLGPNAPTNYALEGSIAIAGAAVQWLRDSLGLISSASEIEKLASEVD 1067
            I S HGLL+T+AFKLG  APTNYALEGSIAIAGAAVQWLRDSLG+IS+ASEIE+LA++VD
Sbjct: 289  IESKHGLLTTLAFKLGREAPTNYALEGSIAIAGAAVQWLRDSLGIISTASEIEELAAKVD 348

Query: 1068 STGGVYFVPAFNGLFAPWWRDDARGVCIGITRFTNKAHIARAVLESMCFQVKDVLDSMHK 1247
            S+GGVYFVPAFNGLFAPWWRDDARGVCIGITRFTNK+HIARAVLESMCFQVKDVLDSMHK
Sbjct: 349  SSGGVYFVPAFNGLFAPWWRDDARGVCIGITRFTNKSHIARAVLESMCFQVKDVLDSMHK 408

Query: 1248 DASE----VESKEKFLLRVDGGATVNNLLMQIQADILGTPVVRPADIETTXXXXXXXXXX 1415
            DA E       K +FLLRVDGGAT+NNLLMQIQAD+LG PVVRPADIETT          
Sbjct: 409  DAGEKGEVKNEKGEFLLRVDGGATINNLLMQIQADLLGNPVVRPADIETTALGAAYAAGL 468

Query: 1416 XXXXWKEEEIFSSEERMKIATIFNPVLEDEKRKKKV 1523
                W E+EIF S E++K+AT F P L++E+R KKV
Sbjct: 469  AVGIWTEDEIFDSGEKVKLATTFYPALDEERRNKKV 504



 Score =  245 bits (625), Expect(2) = 0.0
 Identities = 114/142 (80%), Positives = 128/142 (90%)
 Frame = +1

Query: 4   MSKSDVLIGSLDQGTTSTRFIIYDRNAKAIGSHQVEFTQFYPEAGWVEHDPMEILESART 183
           M+K DV +GS+DQGTTSTRFIIYDR+A+ +GSHQVEFTQFYPEAGWVEHDPMEILES R 
Sbjct: 1   MAKEDVFVGSIDQGTTSTRFIIYDRSAQPVGSHQVEFTQFYPEAGWVEHDPMEILESVRV 60

Query: 184 CMTKAIEKVEADGVNVEKGLSAIGITNQRETTVIWSKSSGAPLYNAIVWMDVRTSSICRR 363
           C+ KAI+K  ADG NV+ GL AIG+TNQRETT+IWSKS+G PLY+AIVWMD RTSSICR+
Sbjct: 61  CIEKAIDKATADGHNVDSGLKAIGLTNQRETTLIWSKSTGLPLYHAIVWMDARTSSICRK 120

Query: 364 LEKELTGGRTHFVETCGLPIST 429
           LEKEL GGRTHFVETCGLPIST
Sbjct: 121 LEKELPGGRTHFVETCGLPIST 142


>emb|CAN76048.1| hypothetical protein VITISV_037711 [Vitis vinifera]
          Length = 522

 Score =  532 bits (1371), Expect(2) = 0.0
 Identities = 262/336 (77%), Positives = 292/336 (86%), Gaps = 4/336 (1%)
 Frame = +3

Query: 528  LFGTIDSWLIWNMTGGTDGGLHVTDVSNAARTMLMNLKTLDWDKTALKELGIPSEILPKI 707
            LFGTID+WLIWNMTGG +GG+HVTDVSNA+RTMLMNLKTLDWDK  L  LGI +EILPKI
Sbjct: 169  LFGTIDTWLIWNMTGGLNGGVHVTDVSNASRTMLMNLKTLDWDKPTLDTLGISAEILPKI 228

Query: 708  VSNSEIIGNVTKGWPITGIPIAGCLGDQHAAMVGQACRKGEAKSTYGTGAFILLNTGDQM 887
            VSN+EIIG V KGWPI G+PI+GCLGDQHAAM+GQACRKGEAKSTYGTGAFILLNTG+++
Sbjct: 229  VSNAEIIGTVAKGWPIPGLPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEV 288

Query: 888  INSTHGLLSTVAFKLGPNAPTNYALEGSIAIAGAAVQWLRDSLGLISSASEIEKLASEVD 1067
            I S HGLL+T+AFKLG  APTNYALEGSIAIAGAAVQWLRDSLG+ISSASEIE+LA++VD
Sbjct: 289  IESKHGLLTTLAFKLGREAPTNYALEGSIAIAGAAVQWLRDSLGIISSASEIEELAAKVD 348

Query: 1068 STGGVYFVPAFNGLFAPWWRDDARGVCIGITRFTNKAHIARAVLESMCFQVKDVLDSMHK 1247
            S+GGVYFVPAFNGLFAPWWRDDARGVCIGITRFTNK+HIARAVLESMCFQVKDVLDSMHK
Sbjct: 349  SSGGVYFVPAFNGLFAPWWRDDARGVCIGITRFTNKSHIARAVLESMCFQVKDVLDSMHK 408

Query: 1248 DASE----VESKEKFLLRVDGGATVNNLLMQIQADILGTPVVRPADIETTXXXXXXXXXX 1415
            DA E       K +FLLRVDGGAT+NNLLMQIQAD+LG PVVRPADIETT          
Sbjct: 409  DAGEKGEVKNEKGEFLLRVDGGATINNLLMQIQADLLGNPVVRPADIETTALGAAYAAGL 468

Query: 1416 XXXXWKEEEIFSSEERMKIATIFNPVLEDEKRKKKV 1523
                W E+EIF S E++K+AT F P L++E+R KKV
Sbjct: 469  AVGIWTEDEIFDSGEKVKLATTFYPALDEERRNKKV 504



 Score =  241 bits (615), Expect(2) = 0.0
 Identities = 112/142 (78%), Positives = 127/142 (89%)
 Frame = +1

Query: 4   MSKSDVLIGSLDQGTTSTRFIIYDRNAKAIGSHQVEFTQFYPEAGWVEHDPMEILESART 183
           M+K DV +GS+DQGTTSTRFIIYDR+A+ +GSHQVEFTQFYPEAGWVEHDPMEILES   
Sbjct: 1   MAKEDVFVGSIDQGTTSTRFIIYDRSAQPVGSHQVEFTQFYPEAGWVEHDPMEILESVXV 60

Query: 184 CMTKAIEKVEADGVNVEKGLSAIGITNQRETTVIWSKSSGAPLYNAIVWMDVRTSSICRR 363
           C+ KAI+K  ADG NV+ GL AIG+TNQRETT+IWSKS+G PLY+AIVWMD RTSSICR+
Sbjct: 61  CIXKAIDKATADGHNVDSGLKAIGLTNQRETTLIWSKSTGLPLYHAIVWMDARTSSICRK 120

Query: 364 LEKELTGGRTHFVETCGLPIST 429
           LEK+L GGRTHFVETCGLPIST
Sbjct: 121 LEKKLPGGRTHFVETCGLPIST 142


>ref|XP_004135863.1| PREDICTED: glycerol kinase-like [Cucumis sativus]
            gi|449509293|ref|XP_004163547.1| PREDICTED: glycerol
            kinase-like [Cucumis sativus]
          Length = 523

 Score =  542 bits (1397), Expect(2) = 0.0
 Identities = 266/336 (79%), Positives = 297/336 (88%), Gaps = 4/336 (1%)
 Frame = +3

Query: 528  LFGTIDSWLIWNMTGGTDGGLHVTDVSNAARTMLMNLKTLDWDKTALKELGIPSEILPKI 707
            LFGTID+WLIWN+TGG +GGLHVTDVSNA+RTMLMNLKTLDWDK  L+ LGIP++ILPKI
Sbjct: 170  LFGTIDTWLIWNLTGGVNGGLHVTDVSNASRTMLMNLKTLDWDKPTLETLGIPAQILPKI 229

Query: 708  VSNSEIIGNVTKGWPITGIPIAGCLGDQHAAMVGQACRKGEAKSTYGTGAFILLNTGDQM 887
            VSNSE+IG +++GWPITG+PI+GCLGDQHAAM+GQACRKGEAKSTYGTGAFILLNTG+++
Sbjct: 230  VSNSEVIGKISQGWPITGVPISGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEEV 289

Query: 888  INSTHGLLSTVAFKLGPNAPTNYALEGSIAIAGAAVQWLRDSLGLISSASEIEKLASEVD 1067
            + S HGLL+T+AFKLGP+APTNYALEGSIAIAGAAVQWLRDSLG+ISSA EIEKLAS+V 
Sbjct: 290  VQSKHGLLTTLAFKLGPDAPTNYALEGSIAIAGAAVQWLRDSLGIISSAQEIEKLASQVQ 349

Query: 1068 STGGVYFVPAFNGLFAPWWRDDARGVCIGITRFTNKAHIARAVLESMCFQVKDVLDSMHK 1247
            STGGVYFVPAFNGLFAPWWRDDARGVCIGITRFT+KAHIARAVLESMCFQVKDVLDSMHK
Sbjct: 350  STGGVYFVPAFNGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHK 409

Query: 1248 DASE----VESKEKFLLRVDGGATVNNLLMQIQADILGTPVVRPADIETTXXXXXXXXXX 1415
            DA E       K +FLLRVDGGATVNNLLMQIQAD+LG+PVVRPADIETT          
Sbjct: 410  DAVEKGEVKNEKGEFLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGL 469

Query: 1416 XXXXWKEEEIFSSEERMKIATIFNPVLEDEKRKKKV 1523
                W ++EIF S ER+K AT F PVLE+E RKKKV
Sbjct: 470  AVGIWSKDEIFDSGERVKSATTFYPVLEEELRKKKV 505



 Score =  230 bits (587), Expect(2) = 0.0
 Identities = 104/138 (75%), Positives = 124/138 (89%)
 Frame = +1

Query: 16  DVLIGSLDQGTTSTRFIIYDRNAKAIGSHQVEFTQFYPEAGWVEHDPMEILESARTCMTK 195
           DV +GS+DQGTTS+RFIIYDR+++ IGSHQ EFTQFYPEAGWVEHDP+EILES + CM K
Sbjct: 6   DVFVGSIDQGTTSSRFIIYDRHSRPIGSHQAEFTQFYPEAGWVEHDPVEILESVKVCMAK 65

Query: 196 AIEKVEADGVNVEKGLSAIGITNQRETTVIWSKSSGAPLYNAIVWMDVRTSSICRRLEKE 375
           A++K  A G NV+ GL AIG+TNQRETT++WSKS+G PL++AIVWMD RTSSICR+LEKE
Sbjct: 66  ALDKATAAGYNVDNGLKAIGLTNQRETTLVWSKSTGFPLHHAIVWMDARTSSICRKLEKE 125

Query: 376 LTGGRTHFVETCGLPIST 429
           L+GGRTHFV+TCGLPIST
Sbjct: 126 LSGGRTHFVDTCGLPIST 143


>ref|XP_002327412.1| predicted protein [Populus trichocarpa] gi|222835966|gb|EEE74387.1|
            predicted protein [Populus trichocarpa]
          Length = 519

 Score =  528 bits (1361), Expect(2) = 0.0
 Identities = 260/332 (78%), Positives = 288/332 (86%)
 Frame = +3

Query: 528  LFGTIDSWLIWNMTGGTDGGLHVTDVSNAARTMLMNLKTLDWDKTALKELGIPSEILPKI 707
            LFGT+DSWLIWN+TGG  GGLHVTDVSNA+RTMLMNLKTL+WDK  L+ L IP+EILPKI
Sbjct: 171  LFGTVDSWLIWNLTGGVKGGLHVTDVSNASRTMLMNLKTLEWDKPTLETLKIPAEILPKI 230

Query: 708  VSNSEIIGNVTKGWPITGIPIAGCLGDQHAAMVGQACRKGEAKSTYGTGAFILLNTGDQM 887
            +SNSEIIG +T+GWP+ G PIAGCLGDQHAAMVGQAC+KGEAKSTYGTGAFIL NTG+++
Sbjct: 231  ISNSEIIGKITEGWPLVGFPIAGCLGDQHAAMVGQACKKGEAKSTYGTGAFILRNTGEEV 290

Query: 888  INSTHGLLSTVAFKLGPNAPTNYALEGSIAIAGAAVQWLRDSLGLISSASEIEKLASEVD 1067
            +NSTHGLL+T+AFKLG  AP NYALEGSIAIAGAAVQWLRDSLG+I SA EIE+LAS+VD
Sbjct: 291  VNSTHGLLTTLAFKLGSKAPANYALEGSIAIAGAAVQWLRDSLGVIKSAGEIEELASQVD 350

Query: 1068 STGGVYFVPAFNGLFAPWWRDDARGVCIGITRFTNKAHIARAVLESMCFQVKDVLDSMHK 1247
            +TGGVYFVPAFNGLFAPWWRDDARGVCIGITRFT+KAHIARAVLESMCFQVKDVLDSMHK
Sbjct: 351  TTGGVYFVPAFNGLFAPWWRDDARGVCIGITRFTSKAHIARAVLESMCFQVKDVLDSMHK 410

Query: 1248 DASEVESKEKFLLRVDGGATVNNLLMQIQADILGTPVVRPADIETTXXXXXXXXXXXXXX 1427
            DA+  E  E FLLRVDGGATVNNLLMQIQAD+LG+PVVRPADIETT              
Sbjct: 411  DAAGSEEGE-FLLRVDGGATVNNLLMQIQADLLGSPVVRPADIETTALGAAYAAGLAVGV 469

Query: 1428 WKEEEIFSSEERMKIATIFNPVLEDEKRKKKV 1523
            W EEEIF+S E+ K  T F P L +E RKKKV
Sbjct: 470  WTEEEIFASGEKSKSDTTFRPKLNEELRKKKV 501



 Score =  232 bits (591), Expect(2) = 0.0
 Identities = 104/141 (73%), Positives = 123/141 (87%)
 Frame = +1

Query: 7   SKSDVLIGSLDQGTTSTRFIIYDRNAKAIGSHQVEFTQFYPEAGWVEHDPMEILESARTC 186
           +K    IGS+DQGT+STRFIIYDRN++ +GSHQVEFTQ YP+AGWVEHDPMEILES + C
Sbjct: 4   TKEAAFIGSIDQGTSSTRFIIYDRNSRCVGSHQVEFTQLYPQAGWVEHDPMEILESVKVC 63

Query: 187 MTKAIEKVEADGVNVEKGLSAIGITNQRETTVIWSKSSGAPLYNAIVWMDVRTSSICRRL 366
           M KA++   A+G NV+  L AIG+TNQRETT++WSKS+G PLYNAIVWMDVRT+S+CRRL
Sbjct: 64  MAKAVDNASAEGHNVDAALKAIGLTNQRETTLVWSKSTGRPLYNAIVWMDVRTTSVCRRL 123

Query: 367 EKELTGGRTHFVETCGLPIST 429
           EKE+ GGRTHFVETCGLPIST
Sbjct: 124 EKEMPGGRTHFVETCGLPIST 144


>ref|XP_003604353.1| Glycerol kinase [Medicago truncatula] gi|355505408|gb|AES86550.1|
            Glycerol kinase [Medicago truncatula]
          Length = 519

 Score =  516 bits (1329), Expect(2) = 0.0
 Identities = 248/333 (74%), Positives = 289/333 (86%), Gaps = 1/333 (0%)
 Frame = +3

Query: 528  LFGTIDSWLIWNMTGGTDGGLHVTDVSNAARTMLMNLKTLDWDKTALKELGIPSEILPKI 707
            LFGTID+WLIWN+TGG  GGLHVTD SNA+RTMLMNLKTL+WD++ LK L IP+EILPKI
Sbjct: 169  LFGTIDTWLIWNLTGGVKGGLHVTDASNASRTMLMNLKTLNWDESTLKALQIPAEILPKI 228

Query: 708  VSNSEIIGNVTKGWPITGIPIAGCLGDQHAAMVGQACRKGEAKSTYGTGAFILLNTGDQM 887
            +SNSEIIGNV+ GWP+ GIPIAGCLGDQHAAM+GQACRKGEAKSTYGTGAFILLNTG+ +
Sbjct: 229  ISNSEIIGNVSSGWPVAGIPIAGCLGDQHAAMLGQACRKGEAKSTYGTGAFILLNTGEGV 288

Query: 888  INSTHGLLSTVAFKLGPNAPTNYALEGSIAIAGAAVQWLRDSLGLISSASEIEKLASEVD 1067
            + S HGLLST+A+KLGPNAPTNYALEGS+AIAGAAVQWLRD LGLI++A +IE LA EV+
Sbjct: 289  VKSNHGLLSTIAYKLGPNAPTNYALEGSVAIAGAAVQWLRDGLGLITTAKDIEALALEVE 348

Query: 1068 STGGVYFVPAFNGLFAPWWRDDARGVCIGITRFTNKAHIARAVLESMCFQVKDVLDSMHK 1247
            S GGVYFVPAFNGLFAPWWRDDARGVCIGITR+T+KAHIARAVLES+CFQVKDV+DSMHK
Sbjct: 349  SNGGVYFVPAFNGLFAPWWRDDARGVCIGITRYTSKAHIARAVLESICFQVKDVIDSMHK 408

Query: 1248 DASEVES-KEKFLLRVDGGATVNNLLMQIQADILGTPVVRPADIETTXXXXXXXXXXXXX 1424
            D+   +S K++FLLRVDGGATVNNLLMQ QAD+L T V+RPADIETT             
Sbjct: 409  DSGADQSKKDEFLLRVDGGATVNNLLMQTQADLLATSVIRPADIETTALGAAYAAGLAVG 468

Query: 1425 XWKEEEIFSSEERMKIATIFNPVLEDEKRKKKV 1523
             WKE+ +F S++ +K A++F P++ +E RKKKV
Sbjct: 469  IWKEDFVFDSKDLLKNASVFRPLMVEEIRKKKV 501



 Score =  241 bits (615), Expect(2) = 0.0
 Identities = 108/142 (76%), Positives = 133/142 (93%)
 Frame = +1

Query: 4   MSKSDVLIGSLDQGTTSTRFIIYDRNAKAIGSHQVEFTQFYPEAGWVEHDPMEILESART 183
           MSK DV IG++DQGT+S+RFIIYD++A+ IGSHQVEFTQFYP+AGWVEH+PMEILES + 
Sbjct: 1   MSKEDVFIGAIDQGTSSSRFIIYDKSAQPIGSHQVEFTQFYPQAGWVEHNPMEILESVKV 60

Query: 184 CMTKAIEKVEADGVNVEKGLSAIGITNQRETTVIWSKSSGAPLYNAIVWMDVRTSSICRR 363
           C+TKA++K  A+G NV+KGL AIG+TNQRETT++WSKS+GAPL+NA+VWMDVRT+S+CRR
Sbjct: 61  CITKAVDKATANGFNVDKGLKAIGLTNQRETTLVWSKSTGAPLHNALVWMDVRTTSVCRR 120

Query: 364 LEKELTGGRTHFVETCGLPIST 429
           LEKEL+GGRTHFVE+CGLPIST
Sbjct: 121 LEKELSGGRTHFVESCGLPIST 142