BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00014258
         (1132 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002267490.2| PREDICTED: probable ubiquitin-conjugating en...   446   e-123
gb|ACB12194.1| PHO2 [Nicotiana benthamiana]                           423   e-116
ref|XP_003528454.1| PREDICTED: probable ubiquitin-conjugating en...   417   e-114
ref|XP_003543046.1| PREDICTED: probable ubiquitin-conjugating en...   416   e-114
ref|XP_002329823.1| predicted protein [Populus trichocarpa] gi|2...   414   e-113

>ref|XP_002267490.2| PREDICTED: probable ubiquitin-conjugating enzyme E2 24-like [Vitis
            vinifera] gi|297735992|emb|CBI23966.3| unnamed protein
            product [Vitis vinifera]
          Length = 944

 Score =  446 bits (1148), Expect = e-123
 Identities = 218/327 (66%), Positives = 256/327 (78%), Gaps = 7/327 (2%)
 Frame = -3

Query: 1118 VLELCNLSTVEQPLVTENLQTSAETHSEQNTNHFL----LP--SGSKSSKYFRQFDIVSD 957
            VL  CN  T EQ +V ++LQ S ET  ++          LP  SGSK+ + F QFD+V D
Sbjct: 604  VLNSCNPCTEEQHIVVDDLQASGETSVKEEIKEIGGDKDLPFSSGSKNPEQFSQFDMVCD 663

Query: 956  CSDHHFVDGSGKSLTSSQMKRGWLKKVQQEWSILEKDLPESIYVRMYEERMDLMRAAIVG 777
            CSDHHFVD +GK L  SQ+K GWLKKVQQEWS+LEK+LPE+IYVR+YEERMDL+RAAIVG
Sbjct: 664  CSDHHFVDSAGKGLALSQVKIGWLKKVQQEWSMLEKNLPETIYVRIYEERMDLLRAAIVG 723

Query: 776  APGTPYHDNLFFFDIYLPPGYPHEPPLVHYNSGGLRLNPNLYESGKVCLSLLNTWTGSGS 597
               TPYHD LFFFDI+LPP YPHEPPLVHYNSGGLR+NPNLYESGKVCLSLLNTWTG+G+
Sbjct: 724  PSATPYHDGLFFFDIFLPPEYPHEPPLVHYNSGGLRVNPNLYESGKVCLSLLNTWTGTGT 783

Query: 596  EVWNPGXXXXXXXXXXXXXXXLNEKPYFNEAGYDQQIGRAEGEKNSLSYNESAFLVSCKS 417
            EVWNPG               LNEKPYFNEAGYD+Q+GRAEGEKNS+SYNE+AF+ +C+S
Sbjct: 784  EVWNPGSSTILQVLLSLQALVLNEKPYFNEAGYDKQMGRAEGEKNSISYNENAFIGTCRS 843

Query: 416  MLYLLRRQPKHFEELVKEHFTQRCKYIIQACNAYMEDTPIGCAFD-KKYDEEAQKGSSTG 240
            +LYLLR  PKHFE +V+EHF Q  ++ + AC AYME  P+GCAF+ KK  EE+QKGSSTG
Sbjct: 844  ILYLLRNPPKHFEAIVEEHFNQCSQHFLLACKAYMEGAPVGCAFEHKKTAEESQKGSSTG 903

Query: 239  FKIMLAKLLPKLVEAFSENGVDCSNYI 159
            FKIMLAKL PKLVEAFS  G+DCS ++
Sbjct: 904  FKIMLAKLFPKLVEAFSARGIDCSQFV 930


>gb|ACB12194.1| PHO2 [Nicotiana benthamiana]
          Length = 921

 Score =  423 bits (1088), Expect = e-116
 Identities = 211/328 (64%), Positives = 251/328 (76%), Gaps = 7/328 (2%)
 Frame = -3

Query: 1130 NEIEVLELCNLS----TVEQPLVTENLQTSAETHSE--QNTNHFLLPSGSKSSKYFRQFD 969
            NE EV+EL NL+    T+E   V  +L+   E   E  ++  H+ LPS SK  + FRQFD
Sbjct: 591  NEEEVIELSNLNAGIPTLEVGYVKASLEMELEQEQETTEDQKHYALPSSSKLPEDFRQFD 650

Query: 968  IVSDCSDHHFVDGSGKSLTSSQMKRGWLKKVQQEWSILEKDLPESIYVRMYEERMDLMRA 789
            +V+  SDHHF DG+GK   S Q++RGWLKKVQQEWSILE DLPE+IYVR+YEERMDL+RA
Sbjct: 651  MVTGFSDHHFADGAGKEQLS-QVRRGWLKKVQQEWSILEHDLPETIYVRVYEERMDLLRA 709

Query: 788  AIVGAPGTPYHDNLFFFDIYLPPGYPHEPPLVHYNSGGLRLNPNLYESGKVCLSLLNTWT 609
            AI+GAPGTPYHD LFFFDIYLP  YPHEPP+V Y+SGGLR+NPNLYESGKVCLSLLNTWT
Sbjct: 710  AIIGAPGTPYHDGLFFFDIYLPSNYPHEPPMVSYHSGGLRVNPNLYESGKVCLSLLNTWT 769

Query: 608  GSGSEVWNPGXXXXXXXXXXXXXXXLNEKPYFNEAGYDQQIGRAEGEKNSLSYNESAFLV 429
            GSG+EVWNP                LNEKPYFNEAGYD QIG+A+GEKNS+SYNE+AFLV
Sbjct: 770  GSGNEVWNPKSSTILQVLLSFQALVLNEKPYFNEAGYDAQIGKADGEKNSVSYNENAFLV 829

Query: 428  SCKSMLYLLRRQPKHFEELVKEHFTQRCKYIIQACNAYMEDTPIGCAFD-KKYDEEAQKG 252
            +CKSMLY L + PKHFE LV+EHF +R K+I+ AC AYM+  P+G AF  K  ++E  +G
Sbjct: 830  TCKSMLYQLNKPPKHFEALVQEHFGKRWKHILLACKAYMDGAPVGSAFQPKSQEQELIEG 889

Query: 251  SSTGFKIMLAKLLPKLVEAFSENGVDCS 168
            SSTGFKIML KL PKL+ AF+  G+DCS
Sbjct: 890  SSTGFKIMLGKLFPKLMAAFTGKGIDCS 917


>ref|XP_003528454.1| PREDICTED: probable ubiquitin-conjugating enzyme E2 24-like [Glycine
            max]
          Length = 927

 Score =  417 bits (1073), Expect = e-114
 Identities = 206/323 (63%), Positives = 245/323 (75%), Gaps = 5/323 (1%)
 Frame = -3

Query: 1112 ELCNLSTVEQPLV----TENLQTSAETHSEQNTNHFLLPSGSKSSKYFRQFDIVSDCSDH 945
            E C+L T + P+     TE+  +  E       N F     S SS  F+QFD+  +C DH
Sbjct: 602  ETCDLFTEQHPMTELQYTEDKTSYPENIKIHEKNDFPFSLDSNSSNQFKQFDVKENCPDH 661

Query: 944  HFVDGSGKSLTSSQMKRGWLKKVQQEWSILEKDLPESIYVRMYEERMDLMRAAIVGAPGT 765
            HF    GK L+ SQ+KR W+KKVQQEWSILEK+LPE+IYV  +EERMDLMRAAIVGA GT
Sbjct: 662  HFFV-QGKGLSISQVKRSWVKKVQQEWSILEKNLPETIYVCAFEERMDLMRAAIVGASGT 720

Query: 764  PYHDNLFFFDIYLPPGYPHEPPLVHYNSGGLRLNPNLYESGKVCLSLLNTWTGSGSEVWN 585
            PYH+ LFFFDI  PP YP++PP+VHYNSGGLRLNPNLYESGK+CLSLLNTWTG+G+EVWN
Sbjct: 721  PYHEGLFFFDICFPPEYPNKPPMVHYNSGGLRLNPNLYESGKICLSLLNTWTGTGTEVWN 780

Query: 584  PGXXXXXXXXXXXXXXXLNEKPYFNEAGYDQQIGRAEGEKNSLSYNESAFLVSCKSMLYL 405
            PG               LNEKPYFNEAGYDQQIGRAEGEKNS+SYNE+A LV+ KSM+YL
Sbjct: 781  PGASTILQVLLSLQALVLNEKPYFNEAGYDQQIGRAEGEKNSVSYNENALLVTTKSMMYL 840

Query: 404  LRRQPKHFEELVKEHFTQRCKYIIQACNAYMEDTPIGCAFD-KKYDEEAQKGSSTGFKIM 228
            LR+ PKHFEELV+EHF +R ++I+ AC AY+E   IGCAFD +K + E QKG+STGFKIM
Sbjct: 841  LRKPPKHFEELVEEHFRKRYQHILLACKAYLEGASIGCAFDSEKNEHENQKGTSTGFKIM 900

Query: 227  LAKLLPKLVEAFSENGVDCSNYI 159
            LAKL PKLVEAFS+ G+DCS ++
Sbjct: 901  LAKLFPKLVEAFSDKGIDCSQFV 923


>ref|XP_003543046.1| PREDICTED: probable ubiquitin-conjugating enzyme E2 24-like [Glycine
            max]
          Length = 919

 Score =  416 bits (1070), Expect = e-114
 Identities = 207/323 (64%), Positives = 245/323 (75%), Gaps = 5/323 (1%)
 Frame = -3

Query: 1115 LELCNLSTVEQPLVTENLQTSA---ETHSEQNTNHFLLPSGSKSS-KYFRQFDIVSDCSD 948
            L+ C   +   P+    +Q+S    E       N F +   +K+S    +QFD++ +CSD
Sbjct: 595  LDTCGPESESHPV--SKMQSSGDICEVIRTHKRNDFPVSLDNKNSLDQLKQFDVIDNCSD 652

Query: 947  HHFVDGSGKSLTSSQMKRGWLKKVQQEWSILEKDLPESIYVRMYEERMDLMRAAIVGAPG 768
            HHF+   GK LTSSQ K+GW+KKVQQEWSILEK+LPE+IYVR++EERMDLMRAAIVGA G
Sbjct: 653  HHFIQ-EGKGLTSSQFKKGWVKKVQQEWSILEKNLPETIYVRVFEERMDLMRAAIVGASG 711

Query: 767  TPYHDNLFFFDIYLPPGYPHEPPLVHYNSGGLRLNPNLYESGKVCLSLLNTWTGSGSEVW 588
            TPYHD LFFFDI  PP YP EPP+VHYNSGGLRLNPNLYESGKVCLSLLNTWTG+ +EVW
Sbjct: 712  TPYHDGLFFFDICFPPEYPSEPPMVHYNSGGLRLNPNLYESGKVCLSLLNTWTGTDTEVW 771

Query: 587  NPGXXXXXXXXXXXXXXXLNEKPYFNEAGYDQQIGRAEGEKNSLSYNESAFLVSCKSMLY 408
            NPG               LN+KPYFNEAGYDQQIGRAEGE+NS+SYNE+AFLV+CKS+LY
Sbjct: 772  NPGASTILQVLLSLQALVLNDKPYFNEAGYDQQIGRAEGERNSVSYNENAFLVTCKSILY 831

Query: 407  LLRRQPKHFEELVKEHFTQRCKYIIQACNAYMEDTPIGCAF-DKKYDEEAQKGSSTGFKI 231
            LLR+ PKHFE LV+EHF Q  K+I+ AC AY+E  PIGC F   K + E QKG+STGFKI
Sbjct: 832  LLRKPPKHFEALVEEHFRQCSKHILLACKAYLEGAPIGCGFGGGKAEHENQKGTSTGFKI 891

Query: 230  MLAKLLPKLVEAFSENGVDCSNY 162
            MLAKL PKLVEAFS+ G+DCS +
Sbjct: 892  MLAKLFPKLVEAFSDKGIDCSQF 914


>ref|XP_002329823.1| predicted protein [Populus trichocarpa] gi|222870885|gb|EEF08016.1|
            predicted protein [Populus trichocarpa]
          Length = 924

 Score =  414 bits (1064), Expect = e-113
 Identities = 205/329 (62%), Positives = 242/329 (73%), Gaps = 7/329 (2%)
 Frame = -3

Query: 1127 EIEVLELCNLSTVEQPLVT------ENLQTSAETHSEQNTNHFLLPSGSKSSKYFRQFDI 966
            E E  E C+L    QPLV       E      E + +Q +      S SK  + F QFD+
Sbjct: 591  EKENPEACDLCMEMQPLVAGDMLRFEGTNLKLEINDDQESKEHRSSSASKRPEPFDQFDM 650

Query: 965  VSDCSDHHFVDGSGKSLTSSQMKRGWLKKVQQEWSILEKDLPESIYVRMYEERMDLMRAA 786
            V+ CSDHHF+DG+G     SQ+KRGWL+KVQQEWSILEK+LPESIYVR+YE+RMDL+RAA
Sbjct: 651  VAVCSDHHFLDGAGNVPALSQVKRGWLRKVQQEWSILEKNLPESIYVRIYEDRMDLLRAA 710

Query: 785  IVGAPGTPYHDNLFFFDIYLPPGYPHEPPLVHYNSGGLRLNPNLYESGKVCLSLLNTWTG 606
            IVG+ GTPYHD LFFFDI+LPPGYPHEPPLVHY+SGGLR+NPNLYESGK+CLSLLNTWTG
Sbjct: 711  IVGSNGTPYHDGLFFFDIFLPPGYPHEPPLVHYHSGGLRVNPNLYESGKICLSLLNTWTG 770

Query: 605  SGSEVWNPGXXXXXXXXXXXXXXXLNEKPYFNEAGYDQQIGRAEGEKNSLSYNESAFLVS 426
            +GSEVWNP                LNEKPYFNEAGYD+QIGRAEGEKNS+SYNE+AFL++
Sbjct: 771  TGSEVWNPESSSILQVLLSLQALVLNEKPYFNEAGYDKQIGRAEGEKNSISYNENAFLMT 830

Query: 425  CKSMLYLLRRQPKHFEELVKEHFTQRCKYIIQACNAYMEDTPIGCAFDK-KYDEEAQKGS 249
             KSMLYLLR+ PKHFE L++EH   R + I+ AC +Y+E  P+  A D    + E QKG 
Sbjct: 831  WKSMLYLLRQPPKHFEPLIEEHLKLRSQNILLACKSYLEGAPVAYALDSGSTEHENQKGG 890

Query: 248  STGFKIMLAKLLPKLVEAFSENGVDCSNY 162
            STGFKIML KL PKLVE FS  G+DCS +
Sbjct: 891  STGFKIMLGKLFPKLVETFSGKGIDCSRF 919