BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00014186
         (1284 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI27298.3| unnamed protein product [Vitis vinifera]              520   e-145
ref|XP_003556625.1| PREDICTED: asparagine synthetase domain-cont...   504   e-140
ref|XP_003607813.1| Asparagine synthetase domain-containing prot...   497   e-138
ref|XP_004135145.1| PREDICTED: asparagine synthetase domain-cont...   495   e-138
ref|XP_004155293.1| PREDICTED: asparagine synthetase domain-cont...   493   e-137

>emb|CBI27298.3| unnamed protein product [Vitis vinifera]
          Length = 645

 Score =  520 bits (1339), Expect = e-145
 Identities = 269/381 (70%), Positives = 312/381 (81%), Gaps = 16/381 (4%)
 Frame = -3

Query: 1282 GEVRNHDWTTVMLQELIKWERSSVAPVIDQL---------DLMTLNIQRNP------QTS 1148
            GEV+ H+WT  +L+EL+KWER+ V P   +L         D+ ++ ++R P      QTS
Sbjct: 260  GEVKKHEWTNPILKELVKWERTYVEPKPAELHFMIPSKQPDVHSICLERKPSGLGPMQTS 319

Query: 1147 LSLTSHAVTVLTALRKSVMRRTVLNNICQPVQGDFAEKSNAPVAVLFSGGLDSMILAAIL 968
            + +   A TVL ALR+S+MRRT  + I   V  D  ++   PVA+LFSGGLDSMILAA+L
Sbjct: 320  VPVP--AQTVLIALRESMMRRTTQSAIFPAVTCDLQQEELVPVAILFSGGLDSMILAALL 377

Query: 967  DECLDTKYEIDLLNVSFDGELAPDRVSARAGIKELCAVAPLRRWKLVEIDANLLKLTSET 788
            DECL   Y IDLLNVSFDG+ APDR+SA+AG+KEL  ++PLRRWKLVE+DA+L KLT ET
Sbjct: 378  DECLQPSYGIDLLNVSFDGQSAPDRISAKAGLKELQRISPLRRWKLVEVDADLSKLTQET 437

Query: 787  KHVMSLINPANTYMDLNIGIALWLAASGNGWVDEVIG-IDNDVVHHRVEYKSAAKILLVG 611
            KHVMSLINPA TYMDLNIGIALWLAA G+GWV E IG IDND  + RV+YKS A+ILLVG
Sbjct: 438  KHVMSLINPAKTYMDLNIGIALWLAAGGDGWVHEEIGEIDNDN-YQRVKYKSKARILLVG 496

Query: 610  AGADEQCAGYGRHKTKYRQGSWQRLHEEMKIDMQRIWKRNLGRDDRCVADNGKEARFPFL 431
            +GADEQCAGYGRHKTKYR GSW  L+EEMK+DMQRIWKRNLGRDDRC+ADNGKEARFPFL
Sbjct: 497  SGADEQCAGYGRHKTKYRSGSWLGLNEEMKLDMQRIWKRNLGRDDRCIADNGKEARFPFL 556

Query: 430  DEDVIRTLLDIPLWEIADLDQPSGVGDKKILREVAKLLGLYDAALLPKRAIQFGSRIARE 251
            DEDVIRTLLDIPLWE+A+LDQPSG GDKKILREVA++LG+Y+AA LPKRAIQFGSRIARE
Sbjct: 557  DEDVIRTLLDIPLWEVANLDQPSGTGDKKILREVAQMLGIYEAATLPKRAIQFGSRIARE 616

Query: 250  SNRKNFGSNRAANQASAGSAV 188
            SNRKNFGSNRAANQASAGS V
Sbjct: 617  SNRKNFGSNRAANQASAGSVV 637


>ref|XP_003556625.1| PREDICTED: asparagine synthetase domain-containing protein 1-like
            [Glycine max]
          Length = 629

 Score =  504 bits (1299), Expect = e-140
 Identities = 253/363 (69%), Positives = 300/363 (82%)
 Frame = -3

Query: 1282 GEVRNHDWTTVMLQELIKWERSSVAPVIDQLDLMTLNIQRNPQTSLSLTSHAVTVLTALR 1103
            GEV+ H++T  ML ELIKWER SV P  + L   + +IQ     ++ + +H +  L AL+
Sbjct: 263  GEVKIHEYTNSMLNELIKWERISVEPSSEDLHTFSGSIQ----PAIPMPAHIL--LNALK 316

Query: 1102 KSVMRRTVLNNICQPVQGDFAEKSNAPVAVLFSGGLDSMILAAILDECLDTKYEIDLLNV 923
            +SV+RRT +  + Q V     ++   PVA+LFSGGLDSMILAA+LD+C+D  YEIDLLNV
Sbjct: 317  ESVLRRTSMYTVYQAVISGIRQEKFFPVAILFSGGLDSMILAALLDKCMDPSYEIDLLNV 376

Query: 922  SFDGELAPDRVSARAGIKELCAVAPLRRWKLVEIDANLLKLTSETKHVMSLINPANTYMD 743
            SFDG+LAPDR SA+AG+ EL  VAP R+W+LVEIDA+L  L  ET HVMSLINPANTYMD
Sbjct: 377  SFDGQLAPDRKSAKAGLNELRRVAPSRKWRLVEIDADLSDLVFETSHVMSLINPANTYMD 436

Query: 742  LNIGIALWLAASGNGWVDEVIGIDNDVVHHRVEYKSAAKILLVGAGADEQCAGYGRHKTK 563
            LNIGIALWLAA G+GWV +    DND  H R++YKS AKILLVG+GADEQCAGYGRH+T 
Sbjct: 437  LNIGIALWLAAGGDGWVSDANTYDNDGNHVRIKYKSNAKILLVGSGADEQCAGYGRHRTS 496

Query: 562  YRQGSWQRLHEEMKIDMQRIWKRNLGRDDRCVADNGKEARFPFLDEDVIRTLLDIPLWEI 383
            YR+GSW  LHEEM++DMQRIW+RNLGRDDRC+ADNGKEARFPFLDEDVIR LL+IPLWE+
Sbjct: 497  YRRGSWLGLHEEMRLDMQRIWRRNLGRDDRCIADNGKEARFPFLDEDVIRLLLNIPLWEV 556

Query: 382  ADLDQPSGVGDKKILREVAKLLGLYDAALLPKRAIQFGSRIARESNRKNFGSNRAANQAS 203
            A+LDQPSG+GDK+ILREVA+LLGLY+AA+LPKRAIQFGSRIARESNRKNFGSNRAANQAS
Sbjct: 557  ANLDQPSGIGDKRILREVAELLGLYEAAVLPKRAIQFGSRIARESNRKNFGSNRAANQAS 616

Query: 202  AGS 194
            AGS
Sbjct: 617  AGS 619


>ref|XP_003607813.1| Asparagine synthetase domain-containing protein [Medicago truncatula]
            gi|355508868|gb|AES90010.1| Asparagine synthetase
            domain-containing protein [Medicago truncatula]
          Length = 646

 Score =  497 bits (1280), Expect = e-138
 Identities = 257/379 (67%), Positives = 294/379 (77%), Gaps = 16/379 (4%)
 Frame = -3

Query: 1282 GEVRNHDWTTVMLQELIKWERSSVAPVIDQLDLMTLNIQRNPQ----------------T 1151
            GEV+ H++T   L+ELIKWER SV P  + L        R  Q                T
Sbjct: 261  GEVQIHEYTNSTLKELIKWERISVVPSSENLQTFCPKFSRGQQDVHSASLETVPCEAGST 320

Query: 1150 SLSLTSHAVTVLTALRKSVMRRTVLNNICQPVQGDFAEKSNAPVAVLFSGGLDSMILAAI 971
              S+   A  +L  L++SV+RRT L  I Q V     ++   PVA+LFSGGLDSMILAA+
Sbjct: 321  QNSIPMRAHMLLNVLKESVIRRTSLYTIYQAVISGIKQEKFVPVAILFSGGLDSMILAAL 380

Query: 970  LDECLDTKYEIDLLNVSFDGELAPDRVSARAGIKELCAVAPLRRWKLVEIDANLLKLTSE 791
            LD+CLD  YEIDLL VSFDGELAPDR SA+ G+KEL  VAP RRW+LVEIDA+L  L SE
Sbjct: 381  LDQCLDPTYEIDLLTVSFDGELAPDRKSAKTGLKELKRVAPSRRWRLVEIDADLSDLVSE 440

Query: 790  TKHVMSLINPANTYMDLNIGIALWLAASGNGWVDEVIGIDNDVVHHRVEYKSAAKILLVG 611
            T HVMSLINPANTYMDLNIGIALWLA+ G+GWV      DND  H R++YKS A+ILLVG
Sbjct: 441  TSHVMSLINPANTYMDLNIGIALWLASCGDGWVS---ADDNDDTHARIKYKSNARILLVG 497

Query: 610  AGADEQCAGYGRHKTKYRQGSWQRLHEEMKIDMQRIWKRNLGRDDRCVADNGKEARFPFL 431
            +GADEQCAGYGRH+T +R+GSW  L EEMK+DMQRIWKRNLGRDDRC+ADNGKEARFPFL
Sbjct: 498  SGADEQCAGYGRHRTSFRRGSWLGLDEEMKLDMQRIWKRNLGRDDRCIADNGKEARFPFL 557

Query: 430  DEDVIRTLLDIPLWEIADLDQPSGVGDKKILREVAKLLGLYDAALLPKRAIQFGSRIARE 251
            DEDVIR LL+IPLWE+A+LDQPSG+GDKKILREVA+LLGLY+AA+LPKRAIQFGSRIARE
Sbjct: 558  DEDVIRVLLNIPLWEVANLDQPSGIGDKKILREVAQLLGLYEAAVLPKRAIQFGSRIARE 617

Query: 250  SNRKNFGSNRAANQASAGS 194
            SNRKNFGSNRAANQASAGS
Sbjct: 618  SNRKNFGSNRAANQASAGS 636


>ref|XP_004135145.1| PREDICTED: asparagine synthetase domain-containing protein 1-like
            [Cucumis sativus]
          Length = 659

 Score =  495 bits (1275), Expect = e-138
 Identities = 254/381 (66%), Positives = 300/381 (78%), Gaps = 18/381 (4%)
 Frame = -3

Query: 1282 GEVRNHDWTTVMLQELIKWERSSVAPVIDQLDLMT------LNIQRNPQTSLS------- 1142
            GEV+ H+WT  +L+EL++W+R+ + P  +  D+ T      L+   +  T L        
Sbjct: 272  GEVKRHEWTNELLKELVEWKRTRIVP--EHGDISTPYHKDCLSQHESHSTCLHNLQSASG 329

Query: 1141 -----LTSHAVTVLTALRKSVMRRTVLNNICQPVQGDFAEKSNAPVAVLFSGGLDSMILA 977
                 +++ A  +L ALRKS++RRT L  I Q       +    PVA+LFSGGLDSMILA
Sbjct: 330  NFQAPVSTPAEILLDALRKSLIRRTSLYTIFQGTMSASRKAELGPVAILFSGGLDSMILA 389

Query: 976  AILDECLDTKYEIDLLNVSFDGELAPDRVSARAGIKELCAVAPLRRWKLVEIDANLLKLT 797
            A+LDECLD  YE+DLLNVSFDG  APDR+SA+AG+KEL  +APLRRWKLVEIDA++  LT
Sbjct: 390  ALLDECLDPCYEVDLLNVSFDGCSAPDRISAKAGVKELSRIAPLRRWKLVEIDADMSDLT 449

Query: 796  SETKHVMSLINPANTYMDLNIGIALWLAASGNGWVDEVIGIDNDVVHHRVEYKSAAKILL 617
            SETKHVMSLINPANTYMDLNIGIALWLA+ GNGW+ E    D +  H RV+YKS AKILL
Sbjct: 450  SETKHVMSLINPANTYMDLNIGIALWLASGGNGWIYESADED-EKFHQRVKYKSEAKILL 508

Query: 616  VGAGADEQCAGYGRHKTKYRQGSWQRLHEEMKIDMQRIWKRNLGRDDRCVADNGKEARFP 437
            VG+GADEQCAGYGRH+TKYRQG W  L+EEMK+D+QRIWKRNLGRDDRC+ADNGKEARFP
Sbjct: 509  VGSGADEQCAGYGRHRTKYRQGGWNLLNEEMKLDIQRIWKRNLGRDDRCIADNGKEARFP 568

Query: 436  FLDEDVIRTLLDIPLWEIADLDQPSGVGDKKILREVAKLLGLYDAALLPKRAIQFGSRIA 257
            FLDEDVIRTLL  PLWE+ DL++P+G GDKKILREVAKLLGL++A +LPKRAIQFGSRIA
Sbjct: 569  FLDEDVIRTLLAFPLWEVTDLEKPAGTGDKKILREVAKLLGLHEAGVLPKRAIQFGSRIA 628

Query: 256  RESNRKNFGSNRAANQASAGS 194
            RESNRKNFGSNRAANQASAGS
Sbjct: 629  RESNRKNFGSNRAANQASAGS 649


>ref|XP_004155293.1| PREDICTED: asparagine synthetase domain-containing protein 1-like
            [Cucumis sativus]
          Length = 659

 Score =  493 bits (1268), Expect = e-137
 Identities = 253/381 (66%), Positives = 299/381 (78%), Gaps = 18/381 (4%)
 Frame = -3

Query: 1282 GEVRNHDWTTVMLQELIKWERSSVAPVIDQLDLMT------LNIQRNPQTSLS------- 1142
            GEV+ H+WT  +L+EL++W+R+ + P  +  D+ T      L+   +  T L        
Sbjct: 272  GEVKRHEWTNELLKELVEWKRTRIVP--EHGDISTPYHKDCLSQHESHSTCLHNLQSASG 329

Query: 1141 -----LTSHAVTVLTALRKSVMRRTVLNNICQPVQGDFAEKSNAPVAVLFSGGLDSMILA 977
                 +++ A  +L ALRKS++RRT L  I         +    PVA+LFSGGLDSMILA
Sbjct: 330  NFQAPVSTPAEILLDALRKSLIRRTSLYTIFLGYMSASRKAEPGPVAILFSGGLDSMILA 389

Query: 976  AILDECLDTKYEIDLLNVSFDGELAPDRVSARAGIKELCAVAPLRRWKLVEIDANLLKLT 797
            A+LDECLD  YE+DLLNVSFDG  APDR+SA+AG+KEL  +APLRRWKLVEIDA++  LT
Sbjct: 390  ALLDECLDPCYEVDLLNVSFDGCSAPDRISAKAGVKELSRIAPLRRWKLVEIDADMSDLT 449

Query: 796  SETKHVMSLINPANTYMDLNIGIALWLAASGNGWVDEVIGIDNDVVHHRVEYKSAAKILL 617
            SETKHVMSLINPANTYMDLNIGIALWLA+ GNGW+ E    D +  H RV+YKS AKILL
Sbjct: 450  SETKHVMSLINPANTYMDLNIGIALWLASGGNGWIYESADED-EKFHQRVKYKSEAKILL 508

Query: 616  VGAGADEQCAGYGRHKTKYRQGSWQRLHEEMKIDMQRIWKRNLGRDDRCVADNGKEARFP 437
            VG+GADEQCAGYGRH+TKYRQG W  L+EEMK+D+QRIWKRNLGRDDRC+ADNGKEARFP
Sbjct: 509  VGSGADEQCAGYGRHRTKYRQGGWNLLNEEMKLDIQRIWKRNLGRDDRCIADNGKEARFP 568

Query: 436  FLDEDVIRTLLDIPLWEIADLDQPSGVGDKKILREVAKLLGLYDAALLPKRAIQFGSRIA 257
            FLDEDVIRTLL  PLWE+ DL++P+G GDKKILREVAKLLGL++A +LPKRAIQFGSRIA
Sbjct: 569  FLDEDVIRTLLAFPLWEVTDLEKPAGTGDKKILREVAKLLGLHEAGVLPKRAIQFGSRIA 628

Query: 256  RESNRKNFGSNRAANQASAGS 194
            RESNRKNFGSNRAANQASAGS
Sbjct: 629  RESNRKNFGSNRAANQASAGS 649