BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00014031
         (1302 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002263023.2| PREDICTED: uncharacterized protein LOC100243...   218   e-101
ref|XP_003634913.1| PREDICTED: uncharacterized protein LOC100243...   218   3e-99
ref|XP_002527685.1| conserved hypothetical protein [Ricinus comm...   192   2e-87
ref|XP_002329748.1| predicted protein [Populus trichocarpa] gi|2...   188   2e-87
ref|NP_565065.1| uncharacterized protein [Arabidopsis thaliana] ...   175   5e-82

>ref|XP_002263023.2| PREDICTED: uncharacterized protein LOC100243690 isoform 1 [Vitis
            vinifera] gi|296088813|emb|CBI38263.3| unnamed protein
            product [Vitis vinifera]
          Length = 354

 Score =  218 bits (556), Expect(2) = e-101
 Identities = 112/161 (69%), Positives = 132/161 (81%), Gaps = 4/161 (2%)
 Frame = -3

Query: 1030 SSHPSISAHNGGTYDPELNYVLQLATGSELYELKQILFGKSYFSPLLKSIARPKDIDHVM 851
            SSH SIS +  G +DPEL  VL+LAT SEL+EL++ILFG SYFSPLLKSI+R  D+D+ M
Sbjct: 41   SSHSSISRNGQGAFDPELRPVLELATDSELFELERILFGPSYFSPLLKSISRRADVDYAM 100

Query: 850  VEQDPEERDDFIAMLESRFFFLAADARSTLRGWRPSYRNVLLGVRKKLKIPCSAKLKTED 671
            +E+D EER+DFI+ LESRF FLAADARSTLRGWRPSYRNVLLGVRKKL +PCS+KL TED
Sbjct: 101  IEEDLEEREDFISSLESRFLFLAADARSTLRGWRPSYRNVLLGVRKKLNVPCSSKLSTED 160

Query: 670  LEVEIFLHLLQDDSSDEAGRLTR----SMAPDDKGSLELGL 560
            LEVEIFLHLLQD SS+E+G L++    S A    G+LE GL
Sbjct: 161  LEVEIFLHLLQDYSSEESGALSKSWENSKASTSHGNLEFGL 201



 Score =  177 bits (450), Expect(2) = e-101
 Identities = 94/147 (63%), Positives = 109/147 (74%)
 Frame = -2

Query: 554 LSQGKIEAVSALRVGALELRTMILKGGGILTLAKLYKMLARRLSGKMFLEAANYQMKNEV 375
           LSQ K++AV+AL  GA ELR++ILKGG +LTL K+Y +LARRLSGK+FLEAANYQ+KNEV
Sbjct: 201 LSQWKVQAVAALGAGASELRSIILKGGSMLTLGKIYHLLARRLSGKLFLEAANYQIKNEV 260

Query: 374 IRKGGHLAAINLESXXXXXXXKEGVRGAASRYIGLRSMMTFFGPILWGTLLADVVIQMLG 195
           I+KGG LAAINLES       K+G  GAASRY+GLRS +  FGP+LWGT LADVVIQMLG
Sbjct: 261 IKKGGQLAAINLESRAALLAAKQGFAGAASRYLGLRSTIALFGPVLWGTFLADVVIQMLG 320

Query: 194 TDYXXXXXXXXXXXXXXXXRTYNLPSD 114
           TDY                RTY LPSD
Sbjct: 321 TDYARILRAIYAFAQIRITRTYRLPSD 347


>ref|XP_003634913.1| PREDICTED: uncharacterized protein LOC100243690 isoform 2 [Vitis
            vinifera]
          Length = 346

 Score =  218 bits (556), Expect(2) = 3e-99
 Identities = 112/161 (69%), Positives = 132/161 (81%), Gaps = 4/161 (2%)
 Frame = -3

Query: 1030 SSHPSISAHNGGTYDPELNYVLQLATGSELYELKQILFGKSYFSPLLKSIARPKDIDHVM 851
            SSH SIS +  G +DPEL  VL+LAT SEL+EL++ILFG SYFSPLLKSI+R  D+D+ M
Sbjct: 41   SSHSSISRNGQGAFDPELRPVLELATDSELFELERILFGPSYFSPLLKSISRRADVDYAM 100

Query: 850  VEQDPEERDDFIAMLESRFFFLAADARSTLRGWRPSYRNVLLGVRKKLKIPCSAKLKTED 671
            +E+D EER+DFI+ LESRF FLAADARSTLRGWRPSYRNVLLGVRKKL +PCS+KL TED
Sbjct: 101  IEEDLEEREDFISSLESRFLFLAADARSTLRGWRPSYRNVLLGVRKKLNVPCSSKLSTED 160

Query: 670  LEVEIFLHLLQDDSSDEAGRLTR----SMAPDDKGSLELGL 560
            LEVEIFLHLLQD SS+E+G L++    S A    G+LE GL
Sbjct: 161  LEVEIFLHLLQDYSSEESGALSKSWENSKASTSHGNLEFGL 201



 Score =  171 bits (434), Expect(2) = 3e-99
 Identities = 87/123 (70%), Positives = 102/123 (82%)
 Frame = -2

Query: 554 LSQGKIEAVSALRVGALELRTMILKGGGILTLAKLYKMLARRLSGKMFLEAANYQMKNEV 375
           LSQ K++AV+AL  GA ELR++ILKGG +LTL K+Y +LARRLSGK+FLEAANYQ+KNEV
Sbjct: 201 LSQWKVQAVAALGAGASELRSIILKGGSMLTLGKIYHLLARRLSGKLFLEAANYQIKNEV 260

Query: 374 IRKGGHLAAINLESXXXXXXXKEGVRGAASRYIGLRSMMTFFGPILWGTLLADVVIQMLG 195
           I+KGG LAAINLES       K+G  GAASRY+GLRS +  FGP+LWGT LADVVIQMLG
Sbjct: 261 IKKGGQLAAINLESRAALLAAKQGFAGAASRYLGLRSTIALFGPVLWGTFLADVVIQMLG 320

Query: 194 TDY 186
           TDY
Sbjct: 321 TDY 323


>ref|XP_002527685.1| conserved hypothetical protein [Ricinus communis]
            gi|223532916|gb|EEF34684.1| conserved hypothetical
            protein [Ricinus communis]
          Length = 372

 Score =  192 bits (487), Expect(2) = 2e-87
 Identities = 126/255 (49%), Positives = 147/255 (57%), Gaps = 19/255 (7%)
 Frame = -3

Query: 1264 LQLYRKCMYIPQPQPH---YSTILSLHSSPNFTLISPSLSYLLNLI*HTHRYYITXXXXX 1094
            L L   C   P P P     ST  S H+ P  T I+P LS+                   
Sbjct: 3    LHLPLPCTPAPAPAPAPTPTSTSTSRHTFPVITTINP-LSHF------------------ 43

Query: 1093 XXXXXXXLQVFRRSNQFPVLGS-----------SHPSISAHNGGTYDPELNYVLQLATGS 947
                      F R++ F +L S           SH ++S    GT D EL  VL+LAT S
Sbjct: 44   --------STFSRTDVFQILSSDKKKSLGIIRCSHAAVS----GTLDRELRSVLELATDS 91

Query: 946  ELYELKQILFGKSYFSPLLKSIA-RPKDIDHVMVEQDPEERDDFIAMLESRFFFLAADAR 770
            ELYEL++ILFG SYFSPLLKSI     D+++   E D  ER+ FIA LESRF +LAADAR
Sbjct: 92   ELYELERILFGPSYFSPLLKSITGNGVDVEYSTFEDDMAEREAFIAALESRFLYLAADAR 151

Query: 769  STLRGWRPSYRNVLLGVRKKLKIPCSAKLKTEDLEVEIFLHLLQDDSSDEAGRLTR---- 602
            STLRGWRPSYRNVLL VRKKL IPCS KL TEDLE EIFLHLLQD  S+E+G +      
Sbjct: 152  STLRGWRPSYRNVLLAVRKKLNIPCSRKLPTEDLEAEIFLHLLQDHPSEESGTVPGLWEF 211

Query: 601  SMAPDDKGSLELGLR 557
            S  P D+GSLE+GLR
Sbjct: 212  SEVPSDQGSLEIGLR 226



 Score =  159 bits (401), Expect(2) = 2e-87
 Identities = 84/148 (56%), Positives = 107/148 (72%)
 Frame = -2

Query: 554 LSQGKIEAVSALRVGALELRTMILKGGGILTLAKLYKMLARRLSGKMFLEAANYQMKNEV 375
           L Q   +A++A+++G  EL+++ILKGGG+ TL++ Y++LAR+LSGK+FLEAANYQ+K EV
Sbjct: 225 LRQWNGQAIAAIKLGLAELQSVILKGGGVFTLSRFYQLLARKLSGKVFLEAANYQIKKEV 284

Query: 374 IRKGGHLAAINLESXXXXXXXKEGVRGAASRYIGLRSMMTFFGPILWGTLLADVVIQMLG 195
           I+KGG LAAINLES       K+G+ GAASRY+G RSM+   GP+LWGT LADVVIQMLG
Sbjct: 285 IKKGGQLAAINLESRAALLAAKQGLAGAASRYLGFRSMLALLGPMLWGTFLADVVIQMLG 344

Query: 194 TDYXXXXXXXXXXXXXXXXRTYNLPSDI 111
           TDY                RTY L SDI
Sbjct: 345 TDYARILRAIYAFAKIRITRTYRL-SDI 371


>ref|XP_002329748.1| predicted protein [Populus trichocarpa] gi|222870656|gb|EEF07787.1|
            predicted protein [Populus trichocarpa]
          Length = 355

 Score =  188 bits (477), Expect(2) = 2e-87
 Identities = 103/167 (61%), Positives = 127/167 (76%), Gaps = 6/167 (3%)
 Frame = -3

Query: 1042 PVLGSSHPSISAHN-GGTYDPELNYVLQLATGSELYELKQILFGKSYFSPLLKSIARPK- 869
            P+  SSH S S  +  G +D +L  VL+LAT SELYEL+ ILFG S+FSPLLKSIA  + 
Sbjct: 41   PLRCSSHASFSPKSQDGPFDRDLRSVLELATDSELYELENILFGPSHFSPLLKSIASKRA 100

Query: 868  DIDHVMVEQDPEERDDFIAMLESRFFFLAADARSTLRGWRPSYRNVLLGVRKKLKIPCSA 689
            +ID+ M++QD EER+D I+ LESRFFFLAADARSTLRGWRP+YRNVLL VRKKL I CS+
Sbjct: 101  EIDYAMMDQDMEEREDMISCLESRFFFLAADARSTLRGWRPTYRNVLLTVRKKLSIGCSS 160

Query: 688  KLKTEDLEVEIFLHLLQDDSSDEAGRL----TRSMAPDDKGSLELGL 560
            KL TEDLE EIFLHLL++ +S+++G        S   DD+GSL +GL
Sbjct: 161  KLSTEDLEAEIFLHLLEEYASEQSGTFPGLWELSKTSDDQGSLGIGL 207



 Score =  162 bits (410), Expect(2) = 2e-87
 Identities = 83/147 (56%), Positives = 108/147 (73%)
 Frame = -2

Query: 554 LSQGKIEAVSALRVGALELRTMILKGGGILTLAKLYKMLARRLSGKMFLEAANYQMKNEV 375
           LSQ K++A++A ++GA +L+++ILKGGG+ TL ++Y+ LA++L+GK+FLEAANYQ+K E+
Sbjct: 207 LSQEKVQALAAQKLGAADLQSIILKGGGVFTLTRIYQWLAKKLTGKVFLEAANYQIKKEI 266

Query: 374 IRKGGHLAAINLESXXXXXXXKEGVRGAASRYIGLRSMMTFFGPILWGTLLADVVIQMLG 195
           I+KGG LAAINLES       K+G  GAASRY+GLRSMM+  GP+LWGT LADVVIQMLG
Sbjct: 267 IKKGGQLAAINLESRAALLVAKQGFVGAASRYLGLRSMMSLLGPMLWGTFLADVVIQMLG 326

Query: 194 TDYXXXXXXXXXXXXXXXXRTYNLPSD 114
           TDY                RT  LP D
Sbjct: 327 TDYARILRAIYAFAQIRITRTCRLPCD 353


>ref|NP_565065.1| uncharacterized protein [Arabidopsis thaliana]
           gi|21593462|gb|AAM65429.1| unknown [Arabidopsis
           thaliana] gi|124301178|gb|ABN04841.1| At1g73470
           [Arabidopsis thaliana] gi|332197343|gb|AEE35464.1|
           uncharacterized protein [Arabidopsis thaliana]
          Length = 351

 Score =  175 bits (444), Expect(2) = 5e-82
 Identities = 95/149 (63%), Positives = 109/149 (73%), Gaps = 4/149 (2%)
 Frame = -3

Query: 994 TYDPELNYVLQLATGSELYELKQILFGKSYFSPLLKSIARPKDIDHVMVEQDPEERDDFI 815
           TYDPEL  V +LAT SELYEL++ILFG SYFSPLLKSI      D +M+ QD E RD FI
Sbjct: 57  TYDPELRLVFELATDSELYELEKILFGPSYFSPLLKSIPNKGGGDRLMIGQDIEVRDGFI 116

Query: 814 AMLESRFFFLAADARSTLRGWRPSYRNVLLGVRKKLKIPCSAKLKTEDLEVEIFLHLLQD 635
             LESRF FLAADARSTLRGWRPSYRNVLL VR  L IPCS++L TEDLE EIFL+L+ +
Sbjct: 117 EALESRFLFLAADARSTLRGWRPSYRNVLLAVRNNLNIPCSSQLPTEDLEAEIFLYLVDN 176

Query: 634 DSSDEA----GRLTRSMAPDDKGSLELGL 560
            SS+ +    G    S   + +GSLELGL
Sbjct: 177 FSSEASGVFPGMWENSEVSEAEGSLELGL 205



 Score =  157 bits (396), Expect(2) = 5e-82
 Identities = 80/145 (55%), Positives = 106/145 (73%)
 Frame = -2

Query: 554 LSQGKIEAVSALRVGALELRTMILKGGGILTLAKLYKMLARRLSGKMFLEAANYQMKNEV 375
           LS+ K+E ++AL+VGA E+++MILKGGG++T AK+Y++LA++LSGK+FLEAANYQ++ E+
Sbjct: 205 LSKWKVELLAALQVGATEVQSMILKGGGMITFAKVYQLLAKKLSGKVFLEAANYQIRKEM 264

Query: 374 IRKGGHLAAINLESXXXXXXXKEGVRGAASRYIGLRSMMTFFGPILWGTLLADVVIQMLG 195
           ++KGG  AAINLES       K G  GAASRYIGL++ M   GP++WGTLLAD+VIQML 
Sbjct: 265 LKKGGQFAAINLESRAALLAAKHGFAGAASRYIGLKTAMQLLGPMMWGTLLADLVIQMLE 324

Query: 194 TDYXXXXXXXXXXXXXXXXRTYNLP 120
           TDY                RTY LP
Sbjct: 325 TDYARILRAIYAFAQIRITRTYRLP 349