BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00013985
         (1617 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ACT37362.1| ankyrin repeat-rich protein [Nicotiana benthamiana]    464   0.0  
ref|XP_002269428.1| PREDICTED: probable E3 ubiquitin-protein lig...   449   0.0  
gb|ABS72112.1| ankyrin repeat-rich protein [Solanum tuberosum]        439   e-179
emb|CAN70237.1| hypothetical protein VITISV_014614 [Vitis vinifera]   449   e-177
ref|XP_004149076.1| PREDICTED: probable E3 ubiquitin-protein lig...   429   e-176

>gb|ACT37362.1| ankyrin repeat-rich protein [Nicotiana benthamiana]
          Length = 513

 Score =  464 bits (1195), Expect(2) = 0.0
 Identities = 231/347 (66%), Positives = 267/347 (76%), Gaps = 15/347 (4%)
 Frame = -2

Query: 1181 EEPILEYDQSSLCQVINSAADGGITALHMAALNGHVDSLHLLLELGASVTEVTVEDGTTI 1002
            E+ I E+D  +L +VIN  ADGGITALHMAALNGHV+SL LLL+LGASVT+VTVEDGTTI
Sbjct: 167  EDSIQEFDDFALHEVINRPADGGITALHMAALNGHVESLQLLLDLGASVTKVTVEDGTTI 226

Query: 1001 DLIGAGSTPLHYAACGGNAQCCQILIAKGASVRAVNGNGWTPLAVARSWHRDCLEEILTP 822
            DLIGAGSTPLHYAACGGNAQCCQ+LIA+GAS+ A N NGWTPL VARSWHRD LEEIL+ 
Sbjct: 227  DLIGAGSTPLHYAACGGNAQCCQLLIARGASLNAENANGWTPLMVARSWHRDGLEEILSA 286

Query: 821  RTDRQFPLALPPSPYLCLPFMSIVTIARECGWRSSESLNACMDPCVVCLERKCTVAAEGC 642
            R +RQ P  LP SP+LCLP MSIV IARECGWR+++S + C+DPCVVCLERKC VAAEGC
Sbjct: 287  RPERQ-PRPLP-SPFLCLPLMSIVKIARECGWRTNDSPSTCLDPCVVCLERKCAVAAEGC 344

Query: 641  YHEFCTRCALYLCSTNCTTTVAHGPPGSIACPLCRHGIVTFVKLEGTSAIVKDIARTSLS 462
            +HEFCTRCALYLCST+ T+T AHGPPGSI CPLCRHGIV+F+KL  T+ I+K+ ARTSLS
Sbjct: 345  FHEFCTRCALYLCSTSSTSTAAHGPPGSIPCPLCRHGIVSFIKLADTTPIIKEAARTSLS 404

Query: 461  LSICTCTALGSEPSTLETLFCKPDVQSARFSPSGLSLRSLSCQTFSSLKFNSSLCMGASD 282
            L  C CTA G EP+TLET FCKPD+  +RFSP G S RSLSCQ F +LKF+  LCMG SD
Sbjct: 405  LPFCACTADGQEPTTLETPFCKPDLYCSRFSPLGSSFRSLSCQKFPALKFSPGLCMGTSD 464

Query: 281  ---------------SKCFKXXXXXXXXXSERGKSWLSSLNQCVATG 186
                           ++C +          E  + WL S +Q V TG
Sbjct: 465  TSPSLVPITAEREHLTRCSRSSFRRSTSNVE-ARRWLCSFSQSVETG 510



 Score =  221 bits (562), Expect(2) = 0.0
 Identities = 106/114 (92%), Positives = 110/114 (96%)
 Frame = -1

Query: 1617 ARDGDLQEAKALLEYNPRLARYSTFGVRNSPLHYSAAQGHHEIVSLLLESGVDINLRNYR 1438
            ARDGDLQEAKALLEYNPRL RYSTFGVRNSPLHYSAAQGHH+IV+LLLESGVDINLRNYR
Sbjct: 32   ARDGDLQEAKALLEYNPRLVRYSTFGVRNSPLHYSAAQGHHKIVTLLLESGVDINLRNYR 91

Query: 1437 GQTALMQACQYGHWEVVLKLVLSKANIHRADYLNGGTALHLAALNGHSRCIRIL 1276
            GQTALMQACQYGHWEVV  L+L KANIHRADYLNGGTALHLAALNGHSRCIR+L
Sbjct: 92   GQTALMQACQYGHWEVVQSLILFKANIHRADYLNGGTALHLAALNGHSRCIRLL 145


>ref|XP_002269428.1| PREDICTED: probable E3 ubiquitin-protein ligase XBOS32 [Vitis
            vinifera] gi|297743747|emb|CBI36630.3| unnamed protein
            product [Vitis vinifera]
          Length = 509

 Score =  449 bits (1155), Expect(2) = 0.0
 Identities = 228/350 (65%), Positives = 263/350 (75%), Gaps = 18/350 (5%)
 Frame = -2

Query: 1181 EEPILEYDQSSLCQVINSAADGGITALHMAALNGHVDSLHLLLELGASVTEVTVEDGTTI 1002
            EE I E+D+ +LCQVIN  ADGGITALHMAALNGHV++L LLL+LGAS++EVTVEDGTTI
Sbjct: 159  EESISEFDEGALCQVINRPADGGITALHMAALNGHVETLQLLLDLGASLSEVTVEDGTTI 218

Query: 1001 DLIGAGSTPLHYAACGGNAQCCQILIAKGASVRAVNGNGWTPLAVARSWHRDCLEEILTP 822
            DLIGAGSTPLHYAACGGNAQCCQILIA+GA + A N NGWTPL VARSWHR  LEEIL+ 
Sbjct: 219  DLIGAGSTPLHYAACGGNAQCCQILIARGAYLAAENANGWTPLMVARSWHRGWLEEILST 278

Query: 821  RTDRQFPLALPPSPYLCLPFMSIVTIARECGWRSSESLNACMDPCVVCLERKCTVAAEGC 642
            + + Q       S YL LP MSIV IARECGWR+++SL  C DPCVVCLERKCTVAA+GC
Sbjct: 279  QPEVQSRTI--SSSYLSLPLMSIVKIARECGWRNNDSLPTCQDPCVVCLERKCTVAAQGC 336

Query: 641  YHEFCTRCALYLCSTNCTTTVAHGPPGSIACPLCRHGIVTFVKLEGTSAIVKDIARTSLS 462
             HEFCT+CALYLCS N ++TV+  P GSIACPLCR+GIV+FVKL GT  +VK+IARTSLS
Sbjct: 337  DHEFCTKCALYLCSMNSSSTVSQCPLGSIACPLCRNGIVSFVKLPGTKPLVKEIARTSLS 396

Query: 461  LSICTCTALGSEPSTLETLFCKPDVQSARFSPSGLSLRSLSCQTFSSLKFNSSLCMGASD 282
            LS CTC+  G EP++L T FCKPD   +R SP G S RSLSCQ FSS+K NSSLCMGA D
Sbjct: 397  LSFCTCSGEGPEPTSLTTPFCKPDFHCSRISPLGSSFRSLSCQRFSSVKLNSSLCMGAPD 456

Query: 281  ------------------SKCFKXXXXXXXXXSERGKSWLSSLNQCVATG 186
                              ++C +         SE  +SW S+LNQ VATG
Sbjct: 457  TSPSLVPCTVDRNLRNHLARCSRSGLRRSASHSESRRSWFSALNQYVATG 506



 Score =  219 bits (558), Expect(2) = 0.0
 Identities = 107/114 (93%), Positives = 110/114 (96%)
 Frame = -1

Query: 1617 ARDGDLQEAKALLEYNPRLARYSTFGVRNSPLHYSAAQGHHEIVSLLLESGVDINLRNYR 1438
            ARDGDLQEAKALLEYNPRLARYSTFGVRNSPLHYSAAQGHHEIVSLLL SGVDINLRNYR
Sbjct: 24   ARDGDLQEAKALLEYNPRLARYSTFGVRNSPLHYSAAQGHHEIVSLLLVSGVDINLRNYR 83

Query: 1437 GQTALMQACQYGHWEVVLKLVLSKANIHRADYLNGGTALHLAALNGHSRCIRIL 1276
            GQTALMQACQ+GHWEVV  LVL +ANIHRADYLNGGTALHLAALNGHSRCIR+L
Sbjct: 84   GQTALMQACQHGHWEVVQTLVLFRANIHRADYLNGGTALHLAALNGHSRCIRLL 137


>gb|ABS72112.1| ankyrin repeat-rich protein [Solanum tuberosum]
          Length = 514

 Score =  439 bits (1129), Expect(2) = e-179
 Identities = 224/351 (63%), Positives = 261/351 (74%), Gaps = 16/351 (4%)
 Frame = -2

Query: 1181 EEPILEYDQSSLCQVINSAADGGITALHMAALNGHVDSLHLLLELGASVTEVTVEDGTTI 1002
            E  I E+D  +L +VIN  AD GITALHMAALNGHV+SL LLL+LGASVT+VTVEDGTTI
Sbjct: 167  EGSIQEFDDFALHEVINRPADSGITALHMAALNGHVESLQLLLDLGASVTKVTVEDGTTI 226

Query: 1001 DLIGAGSTPLHYAACGGNAQCCQILIAKGASVRAVNGNGWTPLAVARSWHRDCLEEILTP 822
            DLIGAGSTPLHYAACGGNAQCCQ+LIA+GAS+ A N NGWT L VARSWHRD LEEIL+ 
Sbjct: 227  DLIGAGSTPLHYAACGGNAQCCQLLIARGASLNAENVNGWTSLMVARSWHRDGLEEILST 286

Query: 821  RTDRQFPLALPPSPYLCLPFMSIVTIARECGWRSSESLNA-CMDPCVVCLERKCTVAAEG 645
            R ++Q P  LP SP+LCLP MSIV IARECGWR+ +S ++ C+DPCVVCLERKCTVAAEG
Sbjct: 287  RPEKQ-PRPLP-SPFLCLPLMSIVKIARECGWRTIDSPSSTCLDPCVVCLERKCTVAAEG 344

Query: 644  CYHEFCTRCALYLCSTNCTTTVAHGPPGSIACPLCRHGIVTFVKLEGTSAIVKDIARTSL 465
            C+HEFCTRCALYLCST+ T+T AHGP GSI CPLCRHGIV+FVKL  T  I+K+ ARTSL
Sbjct: 345  CFHEFCTRCALYLCSTSSTSTAAHGPSGSIPCPLCRHGIVSFVKLADTMPIIKEAARTSL 404

Query: 464  SLSICTCTALGSEPSTLETLFCKPDVQSARFSPSGLSLRSLSCQTFSSLKFNSSLCMGAS 285
            SL  C+CTA G EP++LE   CKPD+  +R SP G S RSLSCQ F +LKF+  LCMG  
Sbjct: 405  SLPFCSCTADGQEPTSLEAPLCKPDLYCSRLSPLGSSFRSLSCQKFPALKFSPGLCMGTP 464

Query: 284  D---------------SKCFKXXXXXXXXXSERGKSWLSSLNQCVATGIKY 177
            D               ++C +          E  + WL S +Q V TG  +
Sbjct: 465  DTSPSFVPRTGEREHLTRCSRPIFRRSTSNVE-ARRWLCSFSQSVETGSSF 514



 Score =  217 bits (552), Expect(2) = e-179
 Identities = 104/114 (91%), Positives = 109/114 (95%)
 Frame = -1

Query: 1617 ARDGDLQEAKALLEYNPRLARYSTFGVRNSPLHYSAAQGHHEIVSLLLESGVDINLRNYR 1438
            ARDGD+QEAKALL+YNPRL RYSTFGVRNSPLHYSAAQGHHEIVSLLLESGVDINLRNYR
Sbjct: 32   ARDGDIQEAKALLDYNPRLVRYSTFGVRNSPLHYSAAQGHHEIVSLLLESGVDINLRNYR 91

Query: 1437 GQTALMQACQYGHWEVVLKLVLSKANIHRADYLNGGTALHLAALNGHSRCIRIL 1276
            GQTALMQACQYGHWEVV  L+L  ANIH+ADYLNGGTALHLAALNGHSRCIR+L
Sbjct: 92   GQTALMQACQYGHWEVVQILILFMANIHKADYLNGGTALHLAALNGHSRCIRLL 145


>emb|CAN70237.1| hypothetical protein VITISV_014614 [Vitis vinifera]
          Length = 511

 Score =  449 bits (1155), Expect(2) = e-177
 Identities = 228/350 (65%), Positives = 263/350 (75%), Gaps = 18/350 (5%)
 Frame = -2

Query: 1181 EEPILEYDQSSLCQVINSAADGGITALHMAALNGHVDSLHLLLELGASVTEVTVEDGTTI 1002
            EE I E+D+ +LCQVIN  ADGGITALHMAALNGHV++L LLL+LGAS++EVTVEDGTTI
Sbjct: 161  EESISEFDEGALCQVINRPADGGITALHMAALNGHVETLQLLLDLGASLSEVTVEDGTTI 220

Query: 1001 DLIGAGSTPLHYAACGGNAQCCQILIAKGASVRAVNGNGWTPLAVARSWHRDCLEEILTP 822
            DLIGAGSTPLHYAACGGNAQCCQILIA+GA + A N NGWTPL VARSWHR  LEEIL+ 
Sbjct: 221  DLIGAGSTPLHYAACGGNAQCCQILIARGAYLAAENANGWTPLMVARSWHRGWLEEILST 280

Query: 821  RTDRQFPLALPPSPYLCLPFMSIVTIARECGWRSSESLNACMDPCVVCLERKCTVAAEGC 642
            + + Q       S YL LP MSIV IARECGWR+++SL  C DPCVVCLERKCTVAA+GC
Sbjct: 281  QPEVQSRTI--SSSYLSLPLMSIVKIARECGWRNNDSLPTCQDPCVVCLERKCTVAAQGC 338

Query: 641  YHEFCTRCALYLCSTNCTTTVAHGPPGSIACPLCRHGIVTFVKLEGTSAIVKDIARTSLS 462
             HEFCT+CALYLCS N ++TV+  P GSIACPLCR+GIV+FVKL GT  +VK+IARTSLS
Sbjct: 339  DHEFCTKCALYLCSMNSSSTVSQCPLGSIACPLCRNGIVSFVKLPGTKPLVKEIARTSLS 398

Query: 461  LSICTCTALGSEPSTLETLFCKPDVQSARFSPSGLSLRSLSCQTFSSLKFNSSLCMGASD 282
            LS CTC+  G EP++L T FCKPD   +R SP G S RSLSCQ FSS+K NSSLCMGA D
Sbjct: 399  LSFCTCSGEGPEPTSLTTPFCKPDFHCSRISPLGSSFRSLSCQRFSSVKLNSSLCMGAPD 458

Query: 281  ------------------SKCFKXXXXXXXXXSERGKSWLSSLNQCVATG 186
                              ++C +         SE  +SW S+LNQ VATG
Sbjct: 459  TSPSLVPCTVDRNLRNHLARCSRSGLRRSASHSESRRSWFSALNQYVATG 508



 Score =  201 bits (511), Expect(2) = e-177
 Identities = 101/116 (87%), Positives = 104/116 (89%), Gaps = 2/116 (1%)
 Frame = -1

Query: 1617 ARDGDLQEAKALLEYNPRLARYSTFGVRNSPLHYSAAQGHHEIVSLLLESGVDINLRNYR 1438
            ARDGDLQEAKALLEYNPRLARYSTFGVRNSPLHYSAAQGHHEIVSLLL SGVDINLRNYR
Sbjct: 24   ARDGDLQEAKALLEYNPRLARYSTFGVRNSPLHYSAAQGHHEIVSLLLVSGVDINLRNYR 83

Query: 1437 GQTALMQACQYGHWEVVLKL--VLSKANIHRADYLNGGTALHLAALNGHSRCIRIL 1276
            GQTALMQACQ+GHWE         S+  IHRADYLNGGTALHLAALNGHSRCIR+L
Sbjct: 84   GQTALMQACQHGHWEGCSDTGSFPSQCRIHRADYLNGGTALHLAALNGHSRCIRLL 139


>ref|XP_004149076.1| PREDICTED: probable E3 ubiquitin-protein ligase XBOS32-like [Cucumis
            sativus] gi|449517425|ref|XP_004165746.1| PREDICTED:
            probable E3 ubiquitin-protein ligase XBOS32-like [Cucumis
            sativus]
          Length = 510

 Score =  429 bits (1102), Expect(2) = e-176
 Identities = 218/351 (62%), Positives = 252/351 (71%), Gaps = 19/351 (5%)
 Frame = -2

Query: 1181 EEPILEYDQSSLCQVINSAADGGITALHMAALNGHVDSLHLLLELGASVTEVTVEDGTTI 1002
            EE + +++  +L +V+N  +DGGITALHMAALNGHVDS+ LLL+LGAS  +VTVEDGTTI
Sbjct: 159  EEQVSKFEFGALKEVVNRTSDGGITALHMAALNGHVDSVQLLLDLGASAFQVTVEDGTTI 218

Query: 1001 DLIGAGSTPLHYAACGGNAQCCQILIAKGASVRAVNGNGWTPLAVARSWHRDCLEEILTP 822
            DLIGAGST LHYAACGGNAQCCQ+LIA+GA + A N NGWTPL VARSWHRD L EIL+ 
Sbjct: 219  DLIGAGSTALHYAACGGNAQCCQMLIARGADLTAQNANGWTPLMVARSWHRDWLVEILS- 277

Query: 821  RTDRQFPLALPPSPYLCLPFMSIVTIARECGWRSSESLNACMDPCVVCLERKCTVAAEGC 642
            R        L PSPYL LP MSIV IARECGWRS +SL  C DPCVVCLER+CTVAA+GC
Sbjct: 278  REAGAVATNLVPSPYLTLPLMSIVRIARECGWRSCDSLLTCQDPCVVCLERECTVAAQGC 337

Query: 641  YHEFCTRCALYLCSTNCTTTVAHGPPGSIACPLCRHGIVTFVKLEGTSAIVKDIARTSLS 462
             HEFCTRCALYLCSTNCT++V+HGP GSIACPLCR+GIV+F+KL  T  I K+I RTSLS
Sbjct: 338  DHEFCTRCALYLCSTNCTSSVSHGPTGSIACPLCRNGIVSFIKLPRTKPIAKEITRTSLS 397

Query: 461  LSICTCTALGSEPSTLETLFCKPDVQSARFSPSGLSLRSLSCQTFSSLKFNSSLCMGASD 282
            LS CTC+    EP TL T  CKP+   +R SP G S RSLSCQ F S+K NS+LCMG   
Sbjct: 398  LSFCTCSGEAPEPPTLTTPLCKPEFTCSRISPLGASFRSLSCQRFPSVKLNSNLCMGDPH 457

Query: 281  -------------------SKCFKXXXXXXXXXSERGKSWLSSLNQCVATG 186
                               S+C +           R +SWLS+LNQ V TG
Sbjct: 458  SSSSSLVPCDVDRNMRNHVSRCSRSSGFRRTASEGR-RSWLSALNQYVTTG 507



 Score =  219 bits (558), Expect(2) = e-176
 Identities = 106/114 (92%), Positives = 110/114 (96%)
 Frame = -1

Query: 1617 ARDGDLQEAKALLEYNPRLARYSTFGVRNSPLHYSAAQGHHEIVSLLLESGVDINLRNYR 1438
            ARDGDLQEAKALLEYNPRLARYSTFGVRNSPLHYSAA GHHEIVSLLLESGV+INLRNYR
Sbjct: 24   ARDGDLQEAKALLEYNPRLARYSTFGVRNSPLHYSAAHGHHEIVSLLLESGVEINLRNYR 83

Query: 1437 GQTALMQACQYGHWEVVLKLVLSKANIHRADYLNGGTALHLAALNGHSRCIRIL 1276
            GQTALMQACQ+GHWEVV  L+L KANIHRADYLNGGTALHLAALNGHSRCIR+L
Sbjct: 84   GQTALMQACQHGHWEVVQTLILFKANIHRADYLNGGTALHLAALNGHSRCIRLL 137