BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00013343
         (625 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABA55805.1| delta-8 sphingolipid desaturase [Crepis alpina]        286   3e-75
gb|ADK91077.1| delta-8 sphingolipid desaturase [Helianthus annuus]    285   5e-75
sp|Q43469.1|SLD1_HELAN RecName: Full=Delta(8)-fatty-acid desatur...   281   9e-74
ref|XP_002302613.1| predicted protein [Populus trichocarpa] gi|2...   256   2e-66
ref|XP_004138473.1| PREDICTED: delta(8)-fatty-acid desaturase-li...   255   4e-66

>gb|ABA55805.1| delta-8 sphingolipid desaturase [Crepis alpina]
          Length = 469

 Score =  286 bits (731), Expect = 3e-75
 Identities = 132/204 (64%), Positives = 149/204 (73%)
 Frame = +3

Query: 9   WIQVAYMGHDAGHYQAMSSRGWNKVAGILIGNCITGISIAWWKWXXXXXXXXXXXXXXXX 188
           W+Q+AY+GHDAGHYQ M++RGWNK AGI IGNCITGISIAWWKW                
Sbjct: 172 WMQIAYLGHDAGHYQMMATRGWNKFAGIFIGNCITGISIAWWKWTHNAH----------- 220

Query: 189 XXXXXXXXXXXXXXXXXXXXXXXXHIACNSLDYDPDLQHLPMLAVSDSFFQSLTSKFYNR 368
                                   HIACNSLDYDPDLQHLPMLAVS   F+S+TS FY R
Sbjct: 221 ------------------------HIACNSLDYDPDLQHLPMLAVSSKLFESITSVFYGR 256

Query: 369 KLTFDPVAKFFVSYQHLSYYPVMCVARVNLYLQTLLLLCSKRKIPDRAFNILGTMVFWTW 548
           +LTFD VA+FF+SYQH +YYP+MCV RVNLYLQT+LLLCSKR+IPDR  NI+GT++FWTW
Sbjct: 257 RLTFDSVARFFISYQHYTYYPIMCVTRVNLYLQTILLLCSKRRIPDRTLNIIGTLIFWTW 316

Query: 549 FPLLVSCLPNWTERVLFVLVSFCV 620
           FPLLVSCLPNW ERV FVLVSFCV
Sbjct: 317 FPLLVSCLPNWPERVAFVLVSFCV 340


>gb|ADK91077.1| delta-8 sphingolipid desaturase [Helianthus annuus]
          Length = 466

 Score =  285 bits (729), Expect = 5e-75
 Identities = 137/204 (67%), Positives = 147/204 (72%)
 Frame = +3

Query: 9   WIQVAYMGHDAGHYQAMSSRGWNKVAGILIGNCITGISIAWWKWXXXXXXXXXXXXXXXX 188
           WIQ+AY+GHDAGHYQ MS+RGWNK AGI IGNCITGISIAWWKW                
Sbjct: 169 WIQIAYLGHDAGHYQMMSTRGWNKFAGIFIGNCITGISIAWWKWTHNAH----------- 217

Query: 189 XXXXXXXXXXXXXXXXXXXXXXXXHIACNSLDYDPDLQHLPMLAVSDSFFQSLTSKFYNR 368
                                   HIACNSLDYDPDLQHLPMLAVS   F SLTS FY R
Sbjct: 218 ------------------------HIACNSLDYDPDLQHLPMLAVSSRLFNSLTSVFYGR 253

Query: 369 KLTFDPVAKFFVSYQHLSYYPVMCVARVNLYLQTLLLLCSKRKIPDRAFNILGTMVFWTW 548
           +LTFD +A+FFVSYQH SYYP+MCVARVNLYLQTLLLL SKRK+PD+  NI GT+VFWTW
Sbjct: 254 QLTFDRLARFFVSYQHYSYYPIMCVARVNLYLQTLLLLLSKRKVPDKGLNIFGTLVFWTW 313

Query: 549 FPLLVSCLPNWTERVLFVLVSFCV 620
           FPLLVSCLPNW ERV FVLVSFCV
Sbjct: 314 FPLLVSCLPNWPERVAFVLVSFCV 337


>sp|Q43469.1|SLD1_HELAN RecName: Full=Delta(8)-fatty-acid desaturase; AltName:
           Full=Delta(8)-sphingolipid desaturase
           gi|1040729|emb|CAA60621.1| delta-8 sphingolipid
           desaturase [Helianthus annuus]
          Length = 458

 Score =  281 bits (718), Expect = 9e-74
 Identities = 134/204 (65%), Positives = 147/204 (72%)
 Frame = +3

Query: 9   WIQVAYMGHDAGHYQAMSSRGWNKVAGILIGNCITGISIAWWKWXXXXXXXXXXXXXXXX 188
           W+Q+AY+GHDAGHYQ M++RGWNK AGI IGNCITGISIAWWKW                
Sbjct: 161 WMQIAYLGHDAGHYQMMATRGWNKFAGIFIGNCITGISIAWWKWTHNAH----------- 209

Query: 189 XXXXXXXXXXXXXXXXXXXXXXXXHIACNSLDYDPDLQHLPMLAVSDSFFQSLTSKFYNR 368
                                   HIACNSLDYDPDLQHLPMLAVS   F S+TS FY R
Sbjct: 210 ------------------------HIACNSLDYDPDLQHLPMLAVSSKLFNSITSVFYGR 245

Query: 369 KLTFDPVAKFFVSYQHLSYYPVMCVARVNLYLQTLLLLCSKRKIPDRAFNILGTMVFWTW 548
           +LTFDP+A+FFVSYQH  YYP+MCVARVNLYLQT+LLL SKRKIPDR  NILGT++FWTW
Sbjct: 246 QLTFDPLARFFVSYQHYLYYPIMCVARVNLYLQTILLLISKRKIPDRGLNILGTLIFWTW 305

Query: 549 FPLLVSCLPNWTERVLFVLVSFCV 620
           FPLLVS LPNW ERV FVLVSFCV
Sbjct: 306 FPLLVSRLPNWPERVAFVLVSFCV 329


>ref|XP_002302613.1| predicted protein [Populus trichocarpa] gi|222844339|gb|EEE81886.1|
           predicted protein [Populus trichocarpa]
          Length = 447

 Score =  256 bits (654), Expect = 2e-66
 Identities = 123/206 (59%), Positives = 140/206 (67%)
 Frame = +3

Query: 3   FTWIQVAYMGHDAGHYQAMSSRGWNKVAGILIGNCITGISIAWWKWXXXXXXXXXXXXXX 182
           F WIQ AY+GHD+GHYQ M SRG+NK A  + GN +TGISIAWWKW              
Sbjct: 148 FLWIQSAYIGHDSGHYQVMKSRGYNKFAQFVAGNSLTGISIAWWKWTHNAH--------- 198

Query: 183 XXXXXXXXXXXXXXXXXXXXXXXXXXHIACNSLDYDPDLQHLPMLAVSDSFFQSLTSKFY 362
                                     H+ACNSLDYDPDLQH+P+ AV+ +FF S+ S FY
Sbjct: 199 --------------------------HLACNSLDYDPDLQHIPVFAVNSTFFNSIKSCFY 232

Query: 363 NRKLTFDPVAKFFVSYQHLSYYPVMCVARVNLYLQTLLLLCSKRKIPDRAFNILGTMVFW 542
            R L FDPVA+FFVSYQH ++YPVMCVARVNLYLQT LLL SKRK PDRA NILG ++FW
Sbjct: 233 GRYLDFDPVARFFVSYQHWTFYPVMCVARVNLYLQTFLLLFSKRKFPDRALNILGILIFW 292

Query: 543 TWFPLLVSCLPNWTERVLFVLVSFCV 620
           TWFPLLVSCLPNW ERV+FVL SF V
Sbjct: 293 TWFPLLVSCLPNWPERVMFVLASFAV 318


>ref|XP_004138473.1| PREDICTED: delta(8)-fatty-acid desaturase-like isoform 1 [Cucumis
           sativus] gi|449441408|ref|XP_004138474.1| PREDICTED:
           delta(8)-fatty-acid desaturase-like isoform 2 [Cucumis
           sativus] gi|449495238|ref|XP_004159774.1| PREDICTED:
           delta(8)-fatty-acid desaturase-like isoform 1 [Cucumis
           sativus] gi|449495242|ref|XP_004159775.1| PREDICTED:
           delta(8)-fatty-acid desaturase-like isoform 2 [Cucumis
           sativus]
          Length = 447

 Score =  255 bits (652), Expect = 4e-66
 Identities = 123/205 (60%), Positives = 144/205 (70%)
 Frame = +3

Query: 9   WIQVAYMGHDAGHYQAMSSRGWNKVAGILIGNCITGISIAWWKWXXXXXXXXXXXXXXXX 188
           WIQ AY+GHD+GH   MSSRG+NK+A IL GNC+TGISIAWWKW                
Sbjct: 150 WIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNCLTGISIAWWKWTHNAH----------- 198

Query: 189 XXXXXXXXXXXXXXXXXXXXXXXXHIACNSLDYDPDLQHLPMLAVSDSFFQSLTSKFYNR 368
                                   HI+CNSLD+DPDLQH+P+ AVS SFF++LTS FY R
Sbjct: 199 ------------------------HISCNSLDHDPDLQHIPVFAVSTSFFRTLTSHFYGR 234

Query: 369 KLTFDPVAKFFVSYQHLSYYPVMCVARVNLYLQTLLLLCSKRKIPDRAFNILGTMVFWTW 548
           KL FD +A+FFVSYQH ++YPVMCVARVNL++QTLLLL S RK+PDRA NI+G +VFWTW
Sbjct: 235 KLEFDSLARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTW 294

Query: 549 FPLLVSCLPNWTERVLFVLVSFCVC 623
           FPLLVS LPNW ERV+FVL SF VC
Sbjct: 295 FPLLVSYLPNWPERVMFVLASFAVC 319