BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00013336
         (1782 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFU52650.1| beta-1,3-glucanase 16 [Solanum tuberosum]              689   0.0  
ref|XP_002530371.1| Glucan endo-1,3-beta-glucosidase precursor, ...   677   0.0  
ref|XP_002273826.2| PREDICTED: glucan endo-1,3-beta-glucosidase ...   672   0.0  
emb|CBI38945.3| unnamed protein product [Vitis vinifera]              672   0.0  
ref|XP_002331390.1| predicted protein [Populus trichocarpa] gi|2...   669   0.0  

>gb|AFU52650.1| beta-1,3-glucanase 16 [Solanum tuberosum]
          Length = 482

 Score =  689 bits (1779), Expect = 0.0
 Identities = 323/409 (78%), Positives = 362/409 (88%)
 Frame = +1

Query: 250  VNGIGANWGTQASHQLPPDTVVRLLRDNGIQKVKLFDADYGALRALRNTGIEVMIGIPNE 429
            V+GIGANWGTQ++H+LPP+ VVR+L+DNGIQKVKLFDADY  L+AL  +G+EVM+GIPN+
Sbjct: 25   VSGIGANWGTQSTHRLPPEIVVRMLKDNGIQKVKLFDADYDTLKALGKSGLEVMVGIPND 84

Query: 430  MLSTLAGSLKAAEKWVSTNVSAHMNGNNVNIRYVAVGNEPFLETYNGSFLQTTFPALRNI 609
            MLST+ GSLKAAEKWVS NVS HMN NNVNIRYVAVGNEP+L TYNG++L+TT PA++NI
Sbjct: 85   MLSTM-GSLKAAEKWVSKNVSVHMNDNNVNIRYVAVGNEPYLTTYNGTYLRTTLPAMQNI 143

Query: 610  QIALTKAGHSNQVKVTCPLNADVYETSTLGPSGGDFRAPIHNYMMQIVKFLSDNGAPFTV 789
            Q AL KAG  +QVKVTCPLNADVYE+S   PSGGDFRA IH Y+  +VKFLSDNG PFT+
Sbjct: 144  QTALIKAGLGSQVKVTCPLNADVYESSNTFPSGGDFRAEIHGYVSPLVKFLSDNGCPFTI 203

Query: 790  NIYPFISLYIDSNFPIEYAFFDGNASPINDNGASYTNMFDANYDTLVWALQRNGFANMPI 969
            NIYPFISLYID NFP+EYAFFDGNA+P+ND G  YTNMFDAN+DTLVWALQ+NGF N+PI
Sbjct: 204  NIYPFISLYIDPNFPVEYAFFDGNATPLNDGGTIYTNMFDANHDTLVWALQKNGFGNVPI 263

Query: 970  IVGEVGWPTDGDRNANPLLAQRFNQGFMSHIAGGKGTPMRPGPIDVYLFSLIDEDEKSIQ 1149
            I+GE+GWPTDGDRNAN  LAQRFNQGFM HI+GGKGTPMRPGP+D YLFSLIDED KSIQ
Sbjct: 264  IIGEIGWPTDGDRNANAQLAQRFNQGFMQHISGGKGTPMRPGPVDAYLFSLIDEDAKSIQ 323

Query: 1150 PGNFERHWGIFTYDGQPKYTLNLGTTNSGVLVKARDVKYLERKWCVLKQSASLDDPQVAQ 1329
            PGNFERHWGI TYDG PKY+LNLGTTNSG LV AR+VKYLERKWCVLK +A LDDPQ+A 
Sbjct: 324  PGNFERHWGILTYDGLPKYSLNLGTTNSGSLVPARNVKYLERKWCVLKPNAKLDDPQIAP 383

Query: 1330 SVSYACGLADCTSLGYGTSCGSLDEHANISYAFNSYYQKNNQLDTACKF 1476
            S+SYACGLADCTSLGY TSCG LD   NISYAFNSYYQ NNQLD ACKF
Sbjct: 384  SMSYACGLADCTSLGYQTSCGGLDARGNISYAFNSYYQINNQLDDACKF 432


>ref|XP_002530371.1| Glucan endo-1,3-beta-glucosidase precursor, putative [Ricinus
            communis] gi|223530088|gb|EEF32004.1| Glucan
            endo-1,3-beta-glucosidase precursor, putative [Ricinus
            communis]
          Length = 482

 Score =  677 bits (1747), Expect(2) = 0.0
 Identities = 321/409 (78%), Positives = 359/409 (87%)
 Frame = +1

Query: 250  VNGIGANWGTQASHQLPPDTVVRLLRDNGIQKVKLFDADYGALRALRNTGIEVMIGIPNE 429
            V+GIGANWGTQA+H LPPDTVVRLLR+NGIQKVKLFDADY ALRAL  +GIEVM+GIPN+
Sbjct: 20   VSGIGANWGTQATHPLPPDTVVRLLRENGIQKVKLFDADYDALRALGKSGIEVMVGIPND 79

Query: 430  MLSTLAGSLKAAEKWVSTNVSAHMNGNNVNIRYVAVGNEPFLETYNGSFLQTTFPALRNI 609
            MLS++AGS+KAAEKWV+ NVS H+  NNVNIRYVAVGNEPFLETYNG+FL TTFPAL+N+
Sbjct: 80   MLSSMAGSMKAAEKWVAKNVSTHITTNNVNIRYVAVGNEPFLETYNGTFLSTTFPALQNV 139

Query: 610  QIALTKAGHSNQVKVTCPLNADVYETSTLGPSGGDFRAPIHNYMMQIVKFLSDNGAPFTV 789
            Q AL KAG SNQVKVT PLNADVY +ST  PSGGDFR  IH+ M+ IVKFLSD+G+PFTV
Sbjct: 140  QSALIKAGLSNQVKVTVPLNADVYASSTGFPSGGDFRTDIHDLMINIVKFLSDSGSPFTV 199

Query: 790  NIYPFISLYIDSNFPIEYAFFDGNASPINDNGASYTNMFDANYDTLVWALQRNGFANMPI 969
            NIYPFISLY D NFP+EYAFFDGNA P+ND G SY NMFDANYDTL +ALQ+NGF N+PI
Sbjct: 200  NIYPFISLYSDPNFPVEYAFFDGNAVPVNDGGTSYYNMFDANYDTLAYALQKNGFGNLPI 259

Query: 970  IVGEVGWPTDGDRNANPLLAQRFNQGFMSHIAGGKGTPMRPGPIDVYLFSLIDEDEKSIQ 1149
            IVGE+GWPTDGD+NAN + AQRFNQGFM  I+GGKGTP+RP PID YLFSLIDED KSI 
Sbjct: 260  IVGEIGWPTDGDKNANMIYAQRFNQGFMQRISGGKGTPLRPEPIDAYLFSLIDEDAKSID 319

Query: 1150 PGNFERHWGIFTYDGQPKYTLNLGTTNSGVLVKARDVKYLERKWCVLKQSASLDDPQVAQ 1329
            PGNFERHWGIFT+DG+ KYTLNLGTTNS  LV+A++V+YLERKWCV+K SA LDDP VA 
Sbjct: 320  PGNFERHWGIFTFDGRAKYTLNLGTTNSRALVEAKNVRYLERKWCVMKSSAKLDDPNVAP 379

Query: 1330 SVSYACGLADCTSLGYGTSCGSLDEHANISYAFNSYYQKNNQLDTACKF 1476
            SVSYACGL DCTSLGYGTSCG+LD   NISYA NSYYQ  NQLD+ACKF
Sbjct: 380  SVSYACGLGDCTSLGYGTSCGNLDARGNISYALNSYYQIQNQLDSACKF 428



 Score = 29.6 bits (65), Expect(2) = 0.0
 Identities = 11/15 (73%), Positives = 12/15 (80%)
 Frame = +2

Query: 1580 PYYAGAQRTVNCIHK 1624
            PYY GA+RTV C HK
Sbjct: 452  PYYGGAKRTVGCPHK 466


>ref|XP_002273826.2| PREDICTED: glucan endo-1,3-beta-glucosidase 6-like [Vitis vinifera]
          Length = 617

 Score =  672 bits (1735), Expect = 0.0
 Identities = 321/410 (78%), Positives = 356/410 (86%)
 Frame = +1

Query: 250  VNGIGANWGTQASHQLPPDTVVRLLRDNGIQKVKLFDADYGALRALRNTGIEVMIGIPNE 429
            V+GIGANWGTQA+H LPP+TVV LLRDNGI KVKLFDADYG L+AL  T IEVM+GIPNE
Sbjct: 155  VSGIGANWGTQATHPLPPETVVDLLRDNGIDKVKLFDADYGNLKALGKTRIEVMVGIPNE 214

Query: 430  MLSTLAGSLKAAEKWVSTNVSAHMNGNNVNIRYVAVGNEPFLETYNGSFLQTTFPALRNI 609
            ML++LAGS+K+AEKWV+ NVS HM  NNVNIRYVAVGNEPFLETYNGSFL+TTFPAL+NI
Sbjct: 215  MLASLAGSVKSAEKWVAKNVSEHMTTNNVNIRYVAVGNEPFLETYNGSFLRTTFPALQNI 274

Query: 610  QIALTKAGHSNQVKVTCPLNADVYETSTLGPSGGDFRAPIHNYMMQIVKFLSDNGAPFTV 789
            Q AL KAG  NQVKVT PLNADVY +S+  PSGGDFR  IH+YM+ IVKFL DN APFTV
Sbjct: 275  QSALIKAGLGNQVKVTVPLNADVYASSSGLPSGGDFRTDIHDYMIPIVKFLMDNNAPFTV 334

Query: 790  NIYPFISLYIDSNFPIEYAFFDGNASPINDNGASYTNMFDANYDTLVWALQRNGFANMPI 969
            NIYPFISLYIDSNFP+EYAFFDGNASPIND G  Y NMFDANYDTLVWAL++NGF N+PI
Sbjct: 335  NIYPFISLYIDSNFPVEYAFFDGNASPINDGGTYYYNMFDANYDTLVWALEKNGFGNLPI 394

Query: 970  IVGEVGWPTDGDRNANPLLAQRFNQGFMSHIAGGKGTPMRPGPIDVYLFSLIDEDEKSIQ 1149
            IVGE+GWPTDGD+NAN   AQRFNQGFM+HI+GGKGTP R GP+D YLFSLIDED KSIQ
Sbjct: 395  IVGEIGWPTDGDQNANLDYAQRFNQGFMTHISGGKGTPKRAGPVDAYLFSLIDEDAKSIQ 454

Query: 1150 PGNFERHWGIFTYDGQPKYTLNLGTTNSGVLVKARDVKYLERKWCVLKQSASLDDPQVAQ 1329
            PG+FERHWG+F +DG PKY  NLGTTNSG L+ AR+VK+LE+KWCV+K SA LDDPQVA 
Sbjct: 455  PGSFERHWGVFYFDGSPKYQFNLGTTNSGSLIPARNVKHLEQKWCVMKPSAGLDDPQVAP 514

Query: 1330 SVSYACGLADCTSLGYGTSCGSLDEHANISYAFNSYYQKNNQLDTACKFP 1479
            SV YAC  ADCTSLGY TSCG LD   NISYAFNSYYQ +NQLDTAC+FP
Sbjct: 515  SVQYACEKADCTSLGYQTSCGGLDARGNISYAFNSYYQTHNQLDTACQFP 564


>emb|CBI38945.3| unnamed protein product [Vitis vinifera]
          Length = 483

 Score =  672 bits (1735), Expect = 0.0
 Identities = 321/410 (78%), Positives = 356/410 (86%)
 Frame = +1

Query: 250  VNGIGANWGTQASHQLPPDTVVRLLRDNGIQKVKLFDADYGALRALRNTGIEVMIGIPNE 429
            V+GIGANWGTQA+H LPP+TVV LLRDNGI KVKLFDADYG L+AL  T IEVM+GIPNE
Sbjct: 21   VSGIGANWGTQATHPLPPETVVDLLRDNGIDKVKLFDADYGNLKALGKTRIEVMVGIPNE 80

Query: 430  MLSTLAGSLKAAEKWVSTNVSAHMNGNNVNIRYVAVGNEPFLETYNGSFLQTTFPALRNI 609
            ML++LAGS+K+AEKWV+ NVS HM  NNVNIRYVAVGNEPFLETYNGSFL+TTFPAL+NI
Sbjct: 81   MLASLAGSVKSAEKWVAKNVSEHMTTNNVNIRYVAVGNEPFLETYNGSFLRTTFPALQNI 140

Query: 610  QIALTKAGHSNQVKVTCPLNADVYETSTLGPSGGDFRAPIHNYMMQIVKFLSDNGAPFTV 789
            Q AL KAG  NQVKVT PLNADVY +S+  PSGGDFR  IH+YM+ IVKFL DN APFTV
Sbjct: 141  QSALIKAGLGNQVKVTVPLNADVYASSSGLPSGGDFRTDIHDYMIPIVKFLMDNNAPFTV 200

Query: 790  NIYPFISLYIDSNFPIEYAFFDGNASPINDNGASYTNMFDANYDTLVWALQRNGFANMPI 969
            NIYPFISLYIDSNFP+EYAFFDGNASPIND G  Y NMFDANYDTLVWAL++NGF N+PI
Sbjct: 201  NIYPFISLYIDSNFPVEYAFFDGNASPINDGGTYYYNMFDANYDTLVWALEKNGFGNLPI 260

Query: 970  IVGEVGWPTDGDRNANPLLAQRFNQGFMSHIAGGKGTPMRPGPIDVYLFSLIDEDEKSIQ 1149
            IVGE+GWPTDGD+NAN   AQRFNQGFM+HI+GGKGTP R GP+D YLFSLIDED KSIQ
Sbjct: 261  IVGEIGWPTDGDQNANLDYAQRFNQGFMTHISGGKGTPKRAGPVDAYLFSLIDEDAKSIQ 320

Query: 1150 PGNFERHWGIFTYDGQPKYTLNLGTTNSGVLVKARDVKYLERKWCVLKQSASLDDPQVAQ 1329
            PG+FERHWG+F +DG PKY  NLGTTNSG L+ AR+VK+LE+KWCV+K SA LDDPQVA 
Sbjct: 321  PGSFERHWGVFYFDGSPKYQFNLGTTNSGSLIPARNVKHLEQKWCVMKPSAGLDDPQVAP 380

Query: 1330 SVSYACGLADCTSLGYGTSCGSLDEHANISYAFNSYYQKNNQLDTACKFP 1479
            SV YAC  ADCTSLGY TSCG LD   NISYAFNSYYQ +NQLDTAC+FP
Sbjct: 381  SVQYACEKADCTSLGYQTSCGGLDARGNISYAFNSYYQTHNQLDTACQFP 430


>ref|XP_002331390.1| predicted protein [Populus trichocarpa] gi|222873604|gb|EEF10735.1|
            predicted protein [Populus trichocarpa]
          Length = 451

 Score =  669 bits (1727), Expect = 0.0
 Identities = 322/408 (78%), Positives = 357/408 (87%), Gaps = 1/408 (0%)
 Frame = +1

Query: 259  IGANWGTQASHQLPPDTVVRLLRDNGIQKVKLFDADYGALRALRNTGIEVMIGIPNEMLS 438
            IGANWGTQASH LPP+TVVRLLR+NGIQKVKLFDADY  L+ L  TGIEVM+GIPN++L+
Sbjct: 14   IGANWGTQASHPLPPETVVRLLRENGIQKVKLFDADYDTLKVLGKTGIEVMVGIPNDLLA 73

Query: 439  TLAGSLKAAEKWVSTNVSAHMNGNNVNIRYVAVGNEPFLETYNGSFLQTTFPALRNIQIA 618
            +LAGS+KAAEKWVS NVSAH+  NNVNIRYVAVGNEPFL+TYNGSFL+TTFPAL+N+Q A
Sbjct: 74   SLAGSMKAAEKWVSRNVSAHVTNNNVNIRYVAVGNEPFLQTYNGSFLRTTFPALQNVQSA 133

Query: 619  LTKAGHSNQVKVTCPLNADVYETSTLGPSGGDFRAPIHNYMMQIVKFLSDNGAPFTVNIY 798
            L KAG  N +KVT PLNADVYE+S+  PSGGDFRA IH+ M+ IVKFL+D GAPFTVNIY
Sbjct: 134  LIKAGLGNLIKVTVPLNADVYESSSGLPSGGDFRADIHDLMLTIVKFLNDAGAPFTVNIY 193

Query: 799  PFISLYIDSNFPIEYAFFDGNASPINDNGASYTNMFDANYDTLVWALQRNGFANMPIIVG 978
            PFISLYIDSNFP+EYAFFDGNA+P+ND G SY NMFDANYDTLV ALQ+NGF N+PIIVG
Sbjct: 194  PFISLYIDSNFPVEYAFFDGNANPVNDGGTSYYNMFDANYDTLVNALQKNGFGNLPIIVG 253

Query: 979  EVGWPTDGDRNANPLLAQRFNQGFMSHIAGGKGTPMRPGP-IDVYLFSLIDEDEKSIQPG 1155
            E+GWPTDGDRNAN   A+RFNQGFMSHIA GKGTPMRP   I+ YLFSLIDED KSI PG
Sbjct: 254  EIGWPTDGDRNANVEYARRFNQGFMSHIASGKGTPMRPNAGINAYLFSLIDEDAKSIDPG 313

Query: 1156 NFERHWGIFTYDGQPKYTLNLGTTNSGVLVKARDVKYLERKWCVLKQSASLDDPQVAQSV 1335
            NFERHWGIFT+DG PKY+LNLGTTN+G L+ AR V YLERKWCV+K SA LDDPQVA SV
Sbjct: 314  NFERHWGIFTFDGIPKYSLNLGTTNTGALIPARSVHYLERKWCVMKPSAKLDDPQVAPSV 373

Query: 1336 SYACGLADCTSLGYGTSCGSLDEHANISYAFNSYYQKNNQLDTACKFP 1479
            SYACGLADCTSLGYGTSCG+LD   NISYAFNSY+Q  NQL  ACKFP
Sbjct: 374  SYACGLADCTSLGYGTSCGNLDPRENISYAFNSYFQIQNQLGDACKFP 421