BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00013269
         (1822 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AEQ29025.1| WRKY35 [Panax quinquefolius]                           247   8e-63
gb|AEQ29021.1| WRKY8, partial [Panax quinquefolius]                   244   7e-62
ref|XP_002302070.1| predicted protein [Populus trichocarpa] gi|2...   226   2e-56
gb|ADL36862.1| WRKY domain class transcription factor [Malus x d...   224   5e-56
ref|XP_002306823.1| predicted protein [Populus trichocarpa] gi|2...   223   1e-55

>gb|AEQ29025.1| WRKY35 [Panax quinquefolius]
          Length = 339

 Score =  247 bits (630), Expect = 8e-63
 Identities = 126/197 (63%), Positives = 133/197 (67%)
 Frame = +3

Query: 807  RSNSGISLNFDSSTCTPTMXXXXXXXXXXXVDGSVSKLDGNTFHLIGASRSLDQSSYLHK 986
            RSNSG++LNFDSSTCTPTM            DGSV+ ++GN FHLIGA+RSLDQSSY HK
Sbjct: 165  RSNSGVNLNFDSSTCTPTMSSTRSFISSSSADGSVANMEGNAFHLIGATRSLDQSSYQHK 224

Query: 987  HRCSAKEDEGSVKCGSSGRCRCSKKRKHRVKRSIKVPAISNKLADIPSDEYSWRKYGQKP 1166
             RCSAK D+GSVKCGSSGRC CSKKRKHRVKRSIKVPAISNKLADIP DEYSWRKYGQKP
Sbjct: 225  RRCSAKGDDGSVKCGSSGRCYCSKKRKHRVKRSIKVPAISNKLADIPPDEYSWRKYGQKP 284

Query: 1167 IKGSPHPRGYYKCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVT 1346
            IKGSPHPRGYYKC                                             VT
Sbjct: 285  IKGSPHPRGYYKC----------------------SSMRGCPARKHVERCLEDPSMLIVT 322

Query: 1347 YEGEHNHPRIPVQSTTT 1397
            YEGEHNHPRIPVQSTTT
Sbjct: 323  YEGEHNHPRIPVQSTTT 339



 Score =  162 bits (410), Expect = 3e-37
 Identities = 87/144 (60%), Positives = 100/144 (69%)
 Frame = +1

Query: 250 MEVEIANKVAVESCHRILSLLSQPQDQTQGRNLRVETGEAVIKFKKVVSLLNADLGHARV 429
           MEVE ANK AVESCHR+LSL+SQPQDQ Q RNL VETGEAV+KF+KVVSLLNA LGHARV
Sbjct: 1   MEVEEANKAAVESCHRVLSLISQPQDQIQYRNLVVETGEAVLKFEKVVSLLNAGLGHARV 60

Query: 430 RKVKKIHTHLPKNMLLDNPIDRSDFQPKALQLLLTNSQETSVLDFGXXXXXXXXXXXXXX 609
           RK++KI T  P+N+LLDNPI R D+QPKA+QLL  NS +T + D G              
Sbjct: 61  RKIEKIQTPFPQNILLDNPIGRPDYQPKAIQLLPANSLDTPIHDNGSNVRSTLTLGNSSL 120

Query: 610 XXXXXXXXPIQFSQHTAPPSNYHF 681
                    +Q  Q T PPSNYHF
Sbjct: 121 ELSSNGKNSLQIPQQT-PPSNYHF 143


>gb|AEQ29021.1| WRKY8, partial [Panax quinquefolius]
          Length = 342

 Score =  244 bits (622), Expect = 7e-62
 Identities = 125/194 (64%), Positives = 131/194 (67%)
 Frame = +3

Query: 807  RSNSGISLNFDSSTCTPTMXXXXXXXXXXXVDGSVSKLDGNTFHLIGASRSLDQSSYLHK 986
            RSNSGI+LNFDSSTCTPTM           VDGSV+ ++GN FHLIGA+RSLDQSSY HK
Sbjct: 165  RSNSGINLNFDSSTCTPTMSSTRSFISSLSVDGSVANMEGNAFHLIGATRSLDQSSYQHK 224

Query: 987  HRCSAKEDEGSVKCGSSGRCRCSKKRKHRVKRSIKVPAISNKLADIPSDEYSWRKYGQKP 1166
             RCSAK D+GSVKCGSSGRC CSKKRKHRVKRSIKVPAISNKLADIP DEYSWRKYGQKP
Sbjct: 225  RRCSAKGDDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDEYSWRKYGQKP 284

Query: 1167 IKGSPHPRGYYKCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVT 1346
            IKGSPHPRGYYKC                                             VT
Sbjct: 285  IKGSPHPRGYYKC----------------------SSMRGCPARKHVERCLEDPSMLIVT 322

Query: 1347 YEGEHNHPRIPVQS 1388
            YEGEHNHPRIPVQS
Sbjct: 323  YEGEHNHPRIPVQS 336



 Score =  162 bits (410), Expect = 3e-37
 Identities = 87/144 (60%), Positives = 100/144 (69%)
 Frame = +1

Query: 250 MEVEIANKVAVESCHRILSLLSQPQDQTQGRNLRVETGEAVIKFKKVVSLLNADLGHARV 429
           MEVE ANK AVESCHR+LSL+SQPQDQ Q RNL VETGEAV+KF+KVVSLLNA LGHARV
Sbjct: 1   MEVEEANKAAVESCHRVLSLISQPQDQIQYRNLVVETGEAVLKFEKVVSLLNAGLGHARV 60

Query: 430 RKVKKIHTHLPKNMLLDNPIDRSDFQPKALQLLLTNSQETSVLDFGXXXXXXXXXXXXXX 609
           RK++KI T  P+N+LLDNPI R D+QPKA+QLL  NS +T + D G              
Sbjct: 61  RKIEKIQTPFPQNILLDNPIGRPDYQPKAIQLLPANSLDTPIHDNGSNVRSTLTLGNSSL 120

Query: 610 XXXXXXXXPIQFSQHTAPPSNYHF 681
                    +Q  Q T PPSNYHF
Sbjct: 121 ELSSNGKNSLQIPQQT-PPSNYHF 143


>ref|XP_002302070.1| predicted protein [Populus trichocarpa] gi|222843796|gb|EEE81343.1|
            predicted protein [Populus trichocarpa]
          Length = 351

 Score =  226 bits (576), Expect = 2e-56
 Identities = 114/197 (57%), Positives = 127/197 (64%)
 Frame = +3

Query: 807  RSNSGISLNFDSSTCTPTMXXXXXXXXXXXVDGSVSKLDGNTFHLIGASRSLDQSSYLHK 986
            +SNSGISLNFDSS+CTPTM           +DGSV+ L+G+ FHL+G +RS DQSS  HK
Sbjct: 177  KSNSGISLNFDSSSCTPTMSSTRSFISSLSIDGSVANLEGSAFHLMGPARSSDQSSQQHK 236

Query: 987  HRCSAKEDEGSVKCGSSGRCRCSKKRKHRVKRSIKVPAISNKLADIPSDEYSWRKYGQKP 1166
             +CS + ++GSVKCGSSGRC CSKKRKHRVKRSIKVPAISNKLADIP D+YSWRKYGQKP
Sbjct: 237  RKCSGRGEDGSVKCGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKP 296

Query: 1167 IKGSPHPRGYYKCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVT 1346
            IKGSPHPRGYYKC                                             VT
Sbjct: 297  IKGSPHPRGYYKC----------------------SSMRGCPARKHVERCLEDPSMLIVT 334

Query: 1347 YEGEHNHPRIPVQSTTT 1397
            YEGEHNHPRIP QS  T
Sbjct: 335  YEGEHNHPRIPAQSANT 351



 Score =  120 bits (302), Expect = 9e-25
 Identities = 69/144 (47%), Positives = 89/144 (61%), Gaps = 2/144 (1%)
 Frame = +1

Query: 256 VEIANKVAVESCHRILSLLSQPQDQTQGRNLRVETGEAVIKFKKVVSLLNADLGHARVRK 435
           VE AN+ AVESCHR++SLLSQPQDQ Q RNL VETGEAV +FKKVVSLLN  LGHARVRK
Sbjct: 4   VEEANRAAVESCHRVISLLSQPQDQVQYRNLMVETGEAVFRFKKVVSLLNTGLGHARVRK 63

Query: 436 VKKIHTHLPKNMLLDNPIDRSDFQPKALQLLLTNS--QETSVLDFGXXXXXXXXXXXXXX 609
           +KK+ T L +++LLDN ++R+    K  Q L ++S  +   + + G              
Sbjct: 64  LKKLPTPLSQSILLDNSLNRTGHSSKTPQFLQSSSYLESHPIQELGSSAKNCLSLGTPSL 123

Query: 610 XXXXXXXXPIQFSQHTAPPSNYHF 681
                   P+Q  Q T+  +NY F
Sbjct: 124 ELSSTGKNPLQLGQPTS-AANYQF 146


>gb|ADL36862.1| WRKY domain class transcription factor [Malus x domestica]
          Length = 355

 Score =  224 bits (572), Expect = 5e-56
 Identities = 119/198 (60%), Positives = 129/198 (65%), Gaps = 1/198 (0%)
 Frame = +3

Query: 807  RSNSGISLNFDSSTCTPTMXXXXXXXXXXXVDGSVSKLDGNTFHLIGASRSLDQSSYLHK 986
            RSNSGI+LNFDSS+CTPTM           +DGSV+  DGN+FHLIGA  S DQ+S  HK
Sbjct: 181  RSNSGINLNFDSSSCTPTMSSTRSFISSLSIDGSVANFDGNSFHLIGAPLSSDQNSQ-HK 239

Query: 987  HRCSAKEDEGSVKCG-SSGRCRCSKKRKHRVKRSIKVPAISNKLADIPSDEYSWRKYGQK 1163
             +CSA+ D+GSVKCG SSGRC CSKKRKHRVKRSIKVPAISNKLADIP D+YSWRKYGQK
Sbjct: 240  RKCSARGDDGSVKCGGSSGRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQK 299

Query: 1164 PIKGSPHPRGYYKCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXV 1343
            PIKGSPHPRGYYKC                                             V
Sbjct: 300  PIKGSPHPRGYYKC----------------------SSMRGCPARKHVERCLEEPSMLMV 337

Query: 1344 TYEGEHNHPRIPVQSTTT 1397
            TYEGEHNHPRIP QSTTT
Sbjct: 338  TYEGEHNHPRIPSQSTTT 355



 Score =  122 bits (306), Expect = 3e-25
 Identities = 69/142 (48%), Positives = 83/142 (58%)
 Frame = +1

Query: 253 EVEIANKVAVESCHRILSLLSQPQDQTQGRNLRVETGEAVIKFKKVVSLLNADLGHARVR 432
           EVE ANK AVESCHR+LSLLSQPQ+Q Q RNL VETG+AV +FKKVVS+LN  LGHARVR
Sbjct: 3   EVEEANKTAVESCHRVLSLLSQPQEQVQYRNLTVETGKAVSRFKKVVSILNTGLGHARVR 62

Query: 433 KVKKIHTHLPKNMLLDNPIDRSDFQPKALQLLLTNSQETSVLDFGXXXXXXXXXXXXXXX 612
           K+KK+    P+ +LLDNPI  +D   K    L ++  E    D                 
Sbjct: 63  KLKKLQIPFPERILLDNPISIADRPSKTPHFLQSSFPENPTQDLSLDVKSALCLGNPSLE 122

Query: 613 XXXXXXXPIQFSQHTAPPSNYH 678
                  PI  +Q   PP+ YH
Sbjct: 123 LSTNGKTPIHPAQQ-VPPAQYH 143


>ref|XP_002306823.1| predicted protein [Populus trichocarpa] gi|222856272|gb|EEE93819.1|
            predicted protein [Populus trichocarpa]
          Length = 347

 Score =  223 bits (569), Expect = 1e-55
 Identities = 113/197 (57%), Positives = 127/197 (64%)
 Frame = +3

Query: 807  RSNSGISLNFDSSTCTPTMXXXXXXXXXXXVDGSVSKLDGNTFHLIGASRSLDQSSYLHK 986
            +SNSGISLNFDSS+CTPTM           +DG+V+ L+G+ FHL GA+RS DQSS  HK
Sbjct: 173  KSNSGISLNFDSSSCTPTMSSTRSFISSLSIDGNVANLEGSAFHLTGAARSSDQSSQQHK 232

Query: 987  HRCSAKEDEGSVKCGSSGRCRCSKKRKHRVKRSIKVPAISNKLADIPSDEYSWRKYGQKP 1166
             +CS + ++GS+KCGSS RC CSKKRKHRVKRSIKVPAISNKLADIP D+YSWRKYGQKP
Sbjct: 233  RKCSGRGEDGSMKCGSSVRCHCSKKRKHRVKRSIKVPAISNKLADIPPDDYSWRKYGQKP 292

Query: 1167 IKGSPHPRGYYKCXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVT 1346
            IKGSPHPRGYYKC                                             VT
Sbjct: 293  IKGSPHPRGYYKC----------------------SSMRGCPARKHVERCLEDPSMLIVT 330

Query: 1347 YEGEHNHPRIPVQSTTT 1397
            YEGEHNHPRIP QST T
Sbjct: 331  YEGEHNHPRIPAQSTNT 347



 Score =  124 bits (311), Expect = 8e-26
 Identities = 70/144 (48%), Positives = 89/144 (61%), Gaps = 2/144 (1%)
 Frame = +1

Query: 256 VEIANKVAVESCHRILSLLSQPQDQTQGRNLRVETGEAVIKFKKVVSLLNADLGHARVRK 435
           VE AN+ AVESCHR++SLLSQPQDQ Q RNL VETGEAV +FKKVVSLLN  LGHARVRK
Sbjct: 4   VEEANRAAVESCHRVISLLSQPQDQVQYRNLMVETGEAVFRFKKVVSLLNTGLGHARVRK 63

Query: 436 VKKIHTHLPKNMLLDNPIDRSDFQPKALQLLLTNS--QETSVLDFGXXXXXXXXXXXXXX 609
           +KK+ T L +++LLDNP+  +D   K  Q L ++S  +   + + G              
Sbjct: 64  LKKLPTPLSQSILLDNPLSSTDHPSKTPQFLQSSSYLESQPIQELGSIAKNCLSLGTPSL 123

Query: 610 XXXXXXXXPIQFSQHTAPPSNYHF 681
                   P+Q  Q T P ++Y F
Sbjct: 124 ELSSNGKNPLQLGQPT-PAAHYQF 146