BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00012476
         (1390 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI19293.3| unnamed protein product [Vitis vinifera]              497   e-138
ref|XP_002283711.2| PREDICTED: E3 ubiquitin-protein ligase UPL2-...   494   e-137
ref|XP_002527362.1| E3 ubiquitin protein ligase upl2, putative [...   464   e-128
ref|XP_004163452.1| PREDICTED: LOW QUALITY PROTEIN: E3 ubiquitin...   437   e-120
ref|XP_004152744.1| PREDICTED: E3 ubiquitin-protein ligase UPL2-...   437   e-120

>emb|CBI19293.3| unnamed protein product [Vitis vinifera]
          Length = 1824

 Score =  497 bits (1279), Expect = e-138
 Identities = 272/465 (58%), Positives = 329/465 (70%), Gaps = 3/465 (0%)
 Frame = +1

Query: 4    YNRPPFDMHPRNRRGESSRRGDGIGSILDRSGG-VNTRRSIASKSFETDGAPLVDTEGLK 180
            +NR  F M+ RNRRGESSRRG+GIGS LDR+GG +  RRS+  K  E DGAPLVDTE LK
Sbjct: 901  HNRTLFGMYHRNRRGESSRRGEGIGSSLDRAGGSIVPRRSMGGKLVEADGAPLVDTEALK 960

Query: 181  AMIRLLRVVQPLYKGQLQRLLLNLCAHNETRTALVKILMGLLIIDVRKPPNLLDSSEPSY 360
            AMIRLLRVVQPLYKGQLQRLLLNLCAH+ETR ALVK+LM +L++D RKP N L++SEPSY
Sbjct: 961  AMIRLLRVVQPLYKGQLQRLLLNLCAHSETRIALVKLLMDMLMLDTRKPANHLNTSEPSY 1020

Query: 361  RLYACQSHVAYSRPQYSDGVPPLVSRRALETLTYLARSHPFVAKLFIRLSMP-PSVQDSS 537
            RLYACQSHV YSRPQY DGVPPLVSRR LET+TYLAR+HP+VAK+ ++  +P P +Q+  
Sbjct: 1021 RLYACQSHVMYSRPQYFDGVPPLVSRRILETMTYLARNHPYVAKILLQYRLPHPPLQEPE 1080

Query: 538  GLELTRGKAIMVVDDNDKEKLPQQEEXXXXXXXXXXXXXXXXXRSIAHLEQLLHLLDVII 717
             L+  RGKA+MV++D   +K   QE                  RSIAHLEQLL+LL+VII
Sbjct: 1081 NLDQVRGKAVMVIEDEVVDKKLHQEGYLSVALLLSLLNQPLYLRSIAHLEQLLNLLEVII 1140

Query: 718  DNAESKPISPDVPGPSETDQVP-CQITTLNAEINAGLAGTSSGSDARSSKANDTSKVPVS 894
            D+ ESK    D  GPS T Q    Q++  +AEINA   G  SG    SSK +D+SK    
Sbjct: 1141 DDVESKSSVSDKSGPSSTGQPSGPQVSISDAEINADSGGV-SGVGVTSSKVDDSSKPSAF 1199

Query: 895  SSNRELVSEDVLYNLPQAELRLLCSLLAREGLSDNAYXXXXXXXXXXXXXXXXHCHLFIS 1074
             S+RE  +  VL NLPQ+ELRLLCSLLAREGLSDNAY                HCHLFI+
Sbjct: 1200 GSHRECDAHSVLLNLPQSELRLLCSLLAREGLSDNAYSLVAEVLKKLVAIAPTHCHLFIT 1259

Query: 1075 ELAGSIQNLTKAAMNELHMFGEVEKALLTATSSDGATVLRVLQXXXXXXXXXXVKDKNGQ 1254
            ELA S+QNLTK+AM+ELH FGE EKALL+++SSDGA +LRVL            K+K+ Q
Sbjct: 1260 ELAFSVQNLTKSAMDELHTFGETEKALLSSSSSDGAAILRVLLALSSLVASLNEKEKDQQ 1319

Query: 1255 IRSEREQAAAIVLLGSINAALDPLWLELSICVGKIERYTDSPRNL 1389
            +  E+EQ AA+  +  I+AAL+PLWLELS C+ KIE Y+DS  N+
Sbjct: 1320 VLPEKEQTAALSQVWDIHAALEPLWLELSTCISKIESYSDSATNI 1364


>ref|XP_002283711.2| PREDICTED: E3 ubiquitin-protein ligase UPL2-like [Vitis vinifera]
          Length = 3750

 Score =  494 bits (1273), Expect = e-137
 Identities = 271/461 (58%), Positives = 327/461 (70%), Gaps = 3/461 (0%)
 Frame = +1

Query: 4    YNRPPFDMHPRNRRGESSRRGDGIGSILDRSGG-VNTRRSIASKSFETDGAPLVDTEGLK 180
            +NR  F M+ RNRRGESSRRG+GIGS LDR+GG +  RRS+  K  E DGAPLVDTE LK
Sbjct: 2781 HNRTLFGMYHRNRRGESSRRGEGIGSSLDRAGGSIVPRRSMGGKLVEADGAPLVDTEALK 2840

Query: 181  AMIRLLRVVQPLYKGQLQRLLLNLCAHNETRTALVKILMGLLIIDVRKPPNLLDSSEPSY 360
            AMIRLLRVVQPLYKGQLQRLLLNLCAH+ETR ALVK+LM +L++D RKP N L++SEPSY
Sbjct: 2841 AMIRLLRVVQPLYKGQLQRLLLNLCAHSETRIALVKLLMDMLMLDTRKPANHLNTSEPSY 2900

Query: 361  RLYACQSHVAYSRPQYSDGVPPLVSRRALETLTYLARSHPFVAKLFIRLSMP-PSVQDSS 537
            RLYACQSHV YSRPQY DGVPPLVSRR LET+TYLAR+HP+VAK+ ++  +P P +Q+  
Sbjct: 2901 RLYACQSHVMYSRPQYFDGVPPLVSRRILETMTYLARNHPYVAKILLQYRLPHPPLQEPE 2960

Query: 538  GLELTRGKAIMVVDDNDKEKLPQQEEXXXXXXXXXXXXXXXXXRSIAHLEQLLHLLDVII 717
             L+  RGKA+MV++D   +K   QE                  RSIAHLEQLL+LL+VII
Sbjct: 2961 NLDQVRGKAVMVIEDEVVDKKLHQEGYLSVALLLSLLNQPLYLRSIAHLEQLLNLLEVII 3020

Query: 718  DNAESKPISPDVPGPSETDQVP-CQITTLNAEINAGLAGTSSGSDARSSKANDTSKVPVS 894
            D+ ESK    D  GPS T Q    Q++  +AEINA   G  SG    SSK +D+SK    
Sbjct: 3021 DDVESKSSVSDKSGPSSTGQPSGPQVSISDAEINADSGGV-SGVGVTSSKVDDSSKPSAF 3079

Query: 895  SSNRELVSEDVLYNLPQAELRLLCSLLAREGLSDNAYXXXXXXXXXXXXXXXXHCHLFIS 1074
             S+RE  +  VL NLPQ+ELRLLCSLLAREGLSDNAY                HCHLFI+
Sbjct: 3080 GSHRECDAHSVLLNLPQSELRLLCSLLAREGLSDNAYSLVAEVLKKLVAIAPTHCHLFIT 3139

Query: 1075 ELAGSIQNLTKAAMNELHMFGEVEKALLTATSSDGATVLRVLQXXXXXXXXXXVKDKNGQ 1254
            ELA S+QNLTK+AM+ELH FGE EKALL+++SSDGA +LRVL            K+K+ Q
Sbjct: 3140 ELAFSVQNLTKSAMDELHTFGETEKALLSSSSSDGAAILRVLLALSSLVASLNEKEKDQQ 3199

Query: 1255 IRSEREQAAAIVLLGSINAALDPLWLELSICVGKIERYTDS 1377
            +  E+EQ AA+  +  I+AAL+PLWLELS C+ KIE Y+DS
Sbjct: 3200 VLPEKEQTAALSQVWDIHAALEPLWLELSTCISKIESYSDS 3240


>ref|XP_002527362.1| E3 ubiquitin protein ligase upl2, putative [Ricinus communis]
            gi|223533281|gb|EEF35034.1| E3 ubiquitin protein ligase
            upl2, putative [Ricinus communis]
          Length = 3666

 Score =  464 bits (1194), Expect = e-128
 Identities = 251/463 (54%), Positives = 319/463 (68%), Gaps = 1/463 (0%)
 Frame = +1

Query: 4    YNRPPFDMHPRNRRGESSRRGDGIGSILDRSGGVNTRRSIASKSFETDGAPLVDTEGLKA 183
            +NR  F M+PR+RRGESSRRG+GIG  L+R+G   +RRSI +K  E DGAPLV+TE LKA
Sbjct: 2702 HNRTLFGMYPRSRRGESSRRGEGIGYSLERAG-TGSRRSITTKLVEADGAPLVETESLKA 2760

Query: 184  MIRLLRVVQPLYKGQLQRLLLNLCAHNETRTALVKILMGLLIIDVRKPPNLLDSSEPSYR 363
            MIR+LR+VQPLYKG LQ+LLLNLCAH ETRT+LVKILM +L++D RKP N L+++EPSYR
Sbjct: 2761 MIRVLRIVQPLYKGPLQKLLLNLCAHGETRTSLVKILMDMLMLDTRKPANYLNAAEPSYR 2820

Query: 364  LYACQSHVAYSRPQYSDGVPPLVSRRALETLTYLARSHPFVAKLFIRLSMP-PSVQDSSG 540
            LYACQS+V YSRPQ  DGVPPLVSRR LETLTYLAR+HP+VA++ ++  +P P++Q +  
Sbjct: 2821 LYACQSNVMYSRPQTFDGVPPLVSRRILETLTYLARNHPYVARILLQSRLPLPALQQAEN 2880

Query: 541  LELTRGKAIMVVDDNDKEKLPQQEEXXXXXXXXXXXXXXXXXRSIAHLEQLLHLLDVIID 720
             +  RGKA+MVV++        +E                  RSIAHLEQLL+LL+VIID
Sbjct: 2881 SDKLRGKAVMVVEEFQDNPKHHEEGYISIALLLSLLNQPLYSRSIAHLEQLLNLLEVIID 2940

Query: 721  NAESKPISPDVPGPSETDQVPCQITTLNAEINAGLAGTSSGSDARSSKANDTSKVPVSSS 900
            +AE K    D  G +     P Q++T +A +N  +   S+G    SS A D+SK     +
Sbjct: 2941 SAECKQSLLDKSGAATERPSPHQMSTSDARVNTEVGSVSAGVAISSSTAIDSSKSTTPGA 3000

Query: 901  NRELVSEDVLYNLPQAELRLLCSLLAREGLSDNAYXXXXXXXXXXXXXXXXHCHLFISEL 1080
            N E  ++ VL NLPQAELRLLCS LAREGLSDNAY                H HLF++EL
Sbjct: 3001 NNECDTQSVLLNLPQAELRLLCSFLAREGLSDNAYTLVAEVMKKLVASAPMHSHLFVTEL 3060

Query: 1081 AGSIQNLTKAAMNELHMFGEVEKALLTATSSDGATVLRVLQXXXXXXXXXXVKDKNGQIR 1260
            A ++QNLTK+AMNEL +FGE  KALL  TSSDGA +LRVLQ           K+K+ QI 
Sbjct: 3061 ADAVQNLTKSAMNELRLFGEEVKALLRTTSSDGAAILRVLQALSSLVASLVEKEKDQQIL 3120

Query: 1261 SEREQAAAIVLLGSINAALDPLWLELSICVGKIERYTDSPRNL 1389
            +E+E +A++  L  INAAL+PLWLELS C+ KIE Y++S  +L
Sbjct: 3121 TEKEHSASLSQLSDINAALEPLWLELSTCISKIEGYSESAPDL 3163


>ref|XP_004163452.1| PREDICTED: LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase UPL2-like
            [Cucumis sativus]
          Length = 3666

 Score =  437 bits (1125), Expect = e-120
 Identities = 248/461 (53%), Positives = 311/461 (67%), Gaps = 3/461 (0%)
 Frame = +1

Query: 4    YNRPPFDMHPRNRRGESSRRGDGIGSILDRSGG-VNTRRSIASKSFETDGAPLVDTEGLK 180
            +NR  F M+PRNRRGESSRR +GI   LDR+GG +++RRS+ ++  E DGAPLVDT+ L 
Sbjct: 2703 HNRTLFGMYPRNRRGESSRRVEGISG-LDRTGGSISSRRSLGARLIEADGAPLVDTDALH 2761

Query: 181  AMIRLLRVVQPLYKGQLQRLLLNLCAHNETRTALVKILMGLLIIDVRKPPNLLDSSEPSY 360
            +MIRLLRVVQPLYKGQLQRLLLNLCAHNETRT+LVKILM +L+ D RK  +  +S+E SY
Sbjct: 2762 SMIRLLRVVQPLYKGQLQRLLLNLCAHNETRTSLVKILMDMLLFDRRKLTDQSNSTELSY 2821

Query: 361  RLYACQSHVAYSRPQYSDGVPPLVSRRALETLTYLARSHPFVAKLFIRLS-MPPSVQDSS 537
            RL+ACQ +V YSRPQ+ DG PPLVSRR LETLTYLAR+HP+VAK+ ++   + P++Q S 
Sbjct: 2822 RLFACQRNVIYSRPQFFDGAPPLVSRRVLETLTYLARNHPYVAKILLQFKFLKPTLQGSE 2881

Query: 538  GLELTRGKAIMVVDDNDKEKLPQQEEXXXXXXXXXXXXXXXXXRSIAHLEQLLHLLDVII 717
             +    GKA M V+ N      Q E                  RSIAHLEQLL+LL+VII
Sbjct: 2882 NVYRDCGKAAMAVEQN-----LQAEGYLSIALLLGLLNQPLYLRSIAHLEQLLNLLEVII 2936

Query: 718  DNAESKPISPDVPGPSETDQVPC-QITTLNAEINAGLAGTSSGSDARSSKANDTSKVPVS 894
            DNAESK    +   PS  +Q    ++++ +AE+NA   G SSG    +S     SK   S
Sbjct: 2937 DNAESKSHLSEQSAPSTAEQPAAPEVSSSDAEVNADSGGVSSG--VGTSAKIGGSKTTAS 2994

Query: 895  SSNRELVSEDVLYNLPQAELRLLCSLLAREGLSDNAYXXXXXXXXXXXXXXXXHCHLFIS 1074
            ++N E  S+ +L NLP+AELRLLCSLLAREGLSDN Y                HC LFI+
Sbjct: 2995 AANSECDSQSILANLPEAELRLLCSLLAREGLSDNTYALVAEVMKKLVAISPIHCRLFIT 3054

Query: 1075 ELAGSIQNLTKAAMNELHMFGEVEKALLTATSSDGATVLRVLQXXXXXXXXXXVKDKNGQ 1254
            EL+ S+Q LT++AM+EL MFGE  KALL+ TSSDGA +LRVLQ           K K+  
Sbjct: 3055 ELSESVQKLTRSAMDELRMFGEAVKALLSTTSSDGAAILRVLQALSSLVASLIEKGKDSS 3114

Query: 1255 IRSEREQAAAIVLLGSINAALDPLWLELSICVGKIERYTDS 1377
            I  E+E A+A+ L+  INAAL+PLWLELS C+ KIE Y+DS
Sbjct: 3115 ILPEKEHASALSLVWDINAALEPLWLELSTCISKIESYSDS 3155


>ref|XP_004152744.1| PREDICTED: E3 ubiquitin-protein ligase UPL2-like [Cucumis sativus]
          Length = 3656

 Score =  437 bits (1125), Expect = e-120
 Identities = 248/461 (53%), Positives = 311/461 (67%), Gaps = 3/461 (0%)
 Frame = +1

Query: 4    YNRPPFDMHPRNRRGESSRRGDGIGSILDRSGG-VNTRRSIASKSFETDGAPLVDTEGLK 180
            +NR  F M+PRNRRGESSRR +GI   LDR+GG +++RRS+ ++  E DGAPLVDT+ L 
Sbjct: 2693 HNRTLFGMYPRNRRGESSRRVEGISG-LDRTGGSISSRRSLGARLIEADGAPLVDTDALH 2751

Query: 181  AMIRLLRVVQPLYKGQLQRLLLNLCAHNETRTALVKILMGLLIIDVRKPPNLLDSSEPSY 360
            +MIRLLRVVQPLYKGQLQRLLLNLCAHNETRT+LVKILM +L+ D RK  +  +S+E SY
Sbjct: 2752 SMIRLLRVVQPLYKGQLQRLLLNLCAHNETRTSLVKILMDMLLFDRRKLTDQSNSTELSY 2811

Query: 361  RLYACQSHVAYSRPQYSDGVPPLVSRRALETLTYLARSHPFVAKLFIRLS-MPPSVQDSS 537
            RL+ACQ +V YSRPQ+ DG PPLVSRR LETLTYLAR+HP+VAK+ ++   + P++Q S 
Sbjct: 2812 RLFACQRNVIYSRPQFFDGAPPLVSRRVLETLTYLARNHPYVAKILLQFKFLKPTLQGSE 2871

Query: 538  GLELTRGKAIMVVDDNDKEKLPQQEEXXXXXXXXXXXXXXXXXRSIAHLEQLLHLLDVII 717
             +    GKA M V+ N      Q E                  RSIAHLEQLL+LL+VII
Sbjct: 2872 NVYRDCGKAAMAVEQN-----LQAEGYLSIALLLGLLNQPLYLRSIAHLEQLLNLLEVII 2926

Query: 718  DNAESKPISPDVPGPSETDQVPC-QITTLNAEINAGLAGTSSGSDARSSKANDTSKVPVS 894
            DNAESK    +   PS  +Q    ++++ +AE+NA   G SSG    +S     SK   S
Sbjct: 2927 DNAESKSHLSEQSAPSTAEQPAAPEVSSSDAEVNADSGGVSSG--VGTSAKIGGSKTTAS 2984

Query: 895  SSNRELVSEDVLYNLPQAELRLLCSLLAREGLSDNAYXXXXXXXXXXXXXXXXHCHLFIS 1074
            ++N E  S+ +L NLP+AELRLLCSLLAREGLSDN Y                HC LFI+
Sbjct: 2985 AANSECDSQSILANLPEAELRLLCSLLAREGLSDNTYALVAEVMKKLVAISPIHCRLFIT 3044

Query: 1075 ELAGSIQNLTKAAMNELHMFGEVEKALLTATSSDGATVLRVLQXXXXXXXXXXVKDKNGQ 1254
            EL+ S+Q LT++AM+EL MFGE  KALL+ TSSDGA +LRVLQ           K K+  
Sbjct: 3045 ELSESVQKLTRSAMDELRMFGEAVKALLSTTSSDGAAILRVLQALSSLVASLIEKGKDSS 3104

Query: 1255 IRSEREQAAAIVLLGSINAALDPLWLELSICVGKIERYTDS 1377
            I  E+E A+A+ L+  INAAL+PLWLELS C+ KIE Y+DS
Sbjct: 3105 ILPEKEHASALSLVWDINAALEPLWLELSTCISKIESYSDS 3145