BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00009990
         (1280 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002526974.1| copine, putative [Ricinus communis] gi|22353...   555   e-156
ref|XP_002298557.1| predicted protein [Populus trichocarpa] gi|2...   553   e-155
ref|XP_002271885.1| PREDICTED: E3 ubiquitin-protein ligase RGLG2...   547   e-153
gb|AFN89139.1| RING-type ubiquitin ligase [Mesembryanthemum crys...   545   e-153
ref|XP_004142779.1| PREDICTED: E3 ubiquitin-protein ligase RGLG2...   542   e-152

>ref|XP_002526974.1| copine, putative [Ricinus communis] gi|223533726|gb|EEF35461.1|
            copine, putative [Ricinus communis]
          Length = 461

 Score =  555 bits (1431), Expect = e-156
 Identities = 280/364 (76%), Positives = 308/364 (84%), Gaps = 2/364 (0%)
 Frame = +1

Query: 193  QNYGGRGHEPQKKQLDRRYSRIADNYNSLDEVTAALANAGLESSNLIVGIDFTKSNEWTG 372
            QN+GG     Q++QLDRRYSRIAD+Y SLD+VT ALA AGLESSNLIVGIDFTKSNEWTG
Sbjct: 79   QNFGG-----QRRQLDRRYSRIADDYKSLDQVTEALAQAGLESSNLIVGIDFTKSNEWTG 133

Query: 373  AKSFNRQSLHHIGGVLNPYEQAISIIGKTLASFDEDNLIPCYGFGDASTHDQDVFSFY-E 549
            A+S+NR+SLHHIG  LNPYEQAISIIGKTLA+FDEDNLIPC+GFGDASTHDQDVF FY +
Sbjct: 134  ARSYNRRSLHHIGDGLNPYEQAISIIGKTLAAFDEDNLIPCFGFGDASTHDQDVFCFYPD 193

Query: 550  ERYCNGFEEVLSRYKEIVPNLRLAGPTSFAPIIEMAMTIVEQSNGQYHVLVIIADGQVTR 729
            ER+CNGFEEVLSRY+EIVPNLRLAGPTSFAPIIEMA TIVEQS GQYHVL+IIADGQVTR
Sbjct: 194  ERFCNGFEEVLSRYREIVPNLRLAGPTSFAPIIEMATTIVEQSGGQYHVLLIIADGQVTR 253

Query: 730  SVDTERGHLSPQEQKTVDAIVKASNLPLSIVLVGVGDGPWDMMKEFDDNIPARAFDNFQF 909
            SVDT R  LSPQE++TV+AIV+AS LPLSIVLVGVGDGPWD M+EFDDNIPAR FDNFQF
Sbjct: 254  SVDTARDQLSPQEKRTVEAIVEASKLPLSIVLVGVGDGPWDTMREFDDNIPAREFDNFQF 313

Query: 910  VNFTEIMSKKLSTSRKETEFALSALMEIPSQYKATMELHILGNARGDVPERFALPPPLYS 1089
            VNFTEIMSK++  SRKETEFAL+ALMEIPSQYKAT+EL+ILG  +G++PER ALPPPLY 
Sbjct: 314  VNFTEIMSKQVDASRKETEFALAALMEIPSQYKATIELNILGRHKGNIPERVALPPPLYG 373

Query: 1090 AASLGHSKPSQSTSFK-KXXXXXXXXXXXXXXXXXXXXXFDNQLCPICLSNSKDMAFGCG 1266
            AAS    KPS ST FK                       +DNQLCPICLSN KDMAFGCG
Sbjct: 374  AASFNSPKPSHSTGFKPSVPSFPVDSPPTSSAPTAPSSNYDNQLCPICLSNPKDMAFGCG 433

Query: 1267 HQTC 1278
            HQTC
Sbjct: 434  HQTC 437


>ref|XP_002298557.1| predicted protein [Populus trichocarpa] gi|222845815|gb|EEE83362.1|
            predicted protein [Populus trichocarpa]
          Length = 376

 Score =  553 bits (1424), Expect = e-155
 Identities = 279/352 (79%), Positives = 302/352 (85%), Gaps = 2/352 (0%)
 Frame = +1

Query: 229  KQLDRRYSRIADNYNSLDEVTAALANAGLESSNLIVGIDFTKSNEWTGAKSFNRQSLHHI 408
            ++LDRRYSRIAD+Y SL +VT ALA AGLESSNLIVGIDFTKSNEWTGA+S+NR+ LHHI
Sbjct: 1    RRLDRRYSRIADDYKSLHQVTEALARAGLESSNLIVGIDFTKSNEWTGARSYNRRCLHHI 60

Query: 409  GGVLNPYEQAISIIGKTLASFDEDNLIPCYGFGDASTHDQDVFSFY-EERYCNGFEEVLS 585
            G   NPYEQAISIIGKTLA+FDEDNLIPC+GFGDASTHDQDVFSFY +ER+CNGFEEVLS
Sbjct: 61   GDGFNPYEQAISIIGKTLAAFDEDNLIPCFGFGDASTHDQDVFSFYPDERFCNGFEEVLS 120

Query: 586  RYKEIVPNLRLAGPTSFAPIIEMAMTIVEQSNGQYHVLVIIADGQVTRSVDTERGHLSPQ 765
            RY+EIVPNLRLAGPTSFAPIIEMAMTIVEQS GQYHVL+IIADGQVTRSVDTE G LSPQ
Sbjct: 121  RYREIVPNLRLAGPTSFAPIIEMAMTIVEQSAGQYHVLLIIADGQVTRSVDTEHGQLSPQ 180

Query: 766  EQKTVDAIVKASNLPLSIVLVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMSKKLS 945
            EQKTV+AIV+AS LPLSI+LVGVGDGPWDMM+EFDDNIPAR FDNFQFVNFTEIMSK + 
Sbjct: 181  EQKTVEAIVQASKLPLSIILVGVGDGPWDMMREFDDNIPARDFDNFQFVNFTEIMSKNVD 240

Query: 946  TSRKETEFALSALMEIPSQYKATMELHILGNARGDVPERFALPPPLYSAASLGHSKPSQS 1125
            TSRKETEFALSALMEIPSQYKAT+EL ILG ++G V ER ALPPPLY AAS   SKPS+ 
Sbjct: 241  TSRKETEFALSALMEIPSQYKATIELSILGQSKGIVSERVALPPPLYGAASFSSSKPSRP 300

Query: 1126 TSFK-KXXXXXXXXXXXXXXXXXXXXXFDNQLCPICLSNSKDMAFGCGHQTC 1278
             SFK                       +DNQLCPICLSNSKDMAFGCGHQTC
Sbjct: 301  ASFKPSVPSYQRDSQPFSSAPPATCSTYDNQLCPICLSNSKDMAFGCGHQTC 352


>ref|XP_002271885.1| PREDICTED: E3 ubiquitin-protein ligase RGLG2 [Vitis vinifera]
            gi|296087586|emb|CBI34842.3| unnamed protein product
            [Vitis vinifera]
          Length = 461

 Score =  547 bits (1409), Expect = e-153
 Identities = 277/369 (75%), Positives = 308/369 (83%), Gaps = 5/369 (1%)
 Frame = +1

Query: 187  PPQNYGG-RGHEPQKKQLDRRYSRIADNYNSLDEVTAALANAGLESSNLIVGIDFTKSNE 363
            P QNY   +G+   +++L+R YSRIAD+Y SLDEVTAALA AGLESSNLIVGIDFTKSNE
Sbjct: 69   PQQNYSSSQGNGDNRRRLERMYSRIADDYRSLDEVTAALARAGLESSNLIVGIDFTKSNE 128

Query: 364  WTGAKSFNRQSLHHIGGVLNPYEQAISIIGKTLASFDEDNLIPCYGFGDASTHDQDVFSF 543
            WTGA+SFNRQSLHH+G  LNPY+QAISIIGKTLA+FDEDN+IPC+GFGDASTHDQDVFSF
Sbjct: 129  WTGARSFNRQSLHHVGDGLNPYQQAISIIGKTLAAFDEDNMIPCFGFGDASTHDQDVFSF 188

Query: 544  YE-ERYCNGFEEVLSRYKEIVPNLRLAGPTSFAPIIEMAMTIVEQSNGQYHVLVIIADGQ 720
            YE ER+CNGFEEVLSRY+EIVP LRLAGPTSFAPIIEMAMTIV  S GQYHVLVIIADGQ
Sbjct: 189  YEDERFCNGFEEVLSRYREIVPRLRLAGPTSFAPIIEMAMTIVNHSGGQYHVLVIIADGQ 248

Query: 721  VTRSVDTERGHLSPQEQKTVDAIVKASNLPLSIVLVGVGDGPWDMMKEFDDNIPARAFDN 900
            VTRSVDT  G LSPQEQKTV+AIV+AS LPLSI+LVGVGDGPWDMM+EFDDNIPARAFDN
Sbjct: 249  VTRSVDTAHGQLSPQEQKTVEAIVEASKLPLSIILVGVGDGPWDMMREFDDNIPARAFDN 308

Query: 901  FQFVNFTEIMSKKLSTSRKETEFALSALMEIPSQYKATMELHILGNARGDVPERFALPPP 1080
            FQFVNFTEIMSK +  SRKETEFAL+ALMEIPSQYKAT+EL+ILG  +GD PER  LP P
Sbjct: 309  FQFVNFTEIMSKNVDLSRKETEFALAALMEIPSQYKATIELNILGGRKGDAPERVPLPTP 368

Query: 1081 LYSAAS--LGHSKPSQSTSFK-KXXXXXXXXXXXXXXXXXXXXXFDNQLCPICLSNSKDM 1251
            +Y  A+     SKPS++TSF+                       FDNQLCPICL+N K+M
Sbjct: 369  MYYGATSFFNSSKPSRTTSFQPSVPPYPEDSSPVGTAPPATSSTFDNQLCPICLTNVKNM 428

Query: 1252 AFGCGHQTC 1278
            AFGCGHQTC
Sbjct: 429  AFGCGHQTC 437


>gb|AFN89139.1| RING-type ubiquitin ligase [Mesembryanthemum crystallinum]
          Length = 472

 Score =  545 bits (1405), Expect = e-153
 Identities = 289/431 (67%), Positives = 317/431 (73%), Gaps = 20/431 (4%)
 Frame = +1

Query: 46   WNQQDYVQPPNYAHPAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPPQNYGGRGHEPQ 225
            WNQ +  Q P Y+ P                                P  +Y    HEP 
Sbjct: 24   WNQYENQQQPGYSQP-----YYAPENQYYQPPQQPPTQQPYPPQPYPPHPSYSVPTHEP- 77

Query: 226  KKQLDRRYSRIADNYNSLDEVTAALANAGLESSNLIVGIDFTKSNEWTGAKSFNRQSLHH 405
            KK+LDRRYSRIAD+Y+SLD+VT ALA+AGLESSNLIVGIDFTKSNEWTGAKSFNR+SLHH
Sbjct: 78   KKRLDRRYSRIADDYSSLDQVTEALAHAGLESSNLIVGIDFTKSNEWTGAKSFNRKSLHH 137

Query: 406  IGGVLNPYEQAISIIGKTLASFDEDNLIPCYGFGDASTHDQDVFSFY--EERYCNGFEEV 579
            IG  LNPY+QAI+IIGKTLA+FD+DNLIPCYGFGDASTHDQDVFSFY  E RYCNGFEEV
Sbjct: 138  IGDGLNPYQQAIAIIGKTLAAFDDDNLIPCYGFGDASTHDQDVFSFYPDEGRYCNGFEEV 197

Query: 580  LSRYKEIVPNLRLAGPTSFAPIIEMAMTIVEQSNGQYHVLVIIADGQVTRSVDTERGHLS 759
            LSRY+EIVPNLRLAGPTSFAP+IEMAMTIVEQS GQYHVL+IIADGQVTRSVDTERG LS
Sbjct: 198  LSRYREIVPNLRLAGPTSFAPVIEMAMTIVEQSGGQYHVLLIIADGQVTRSVDTERGRLS 257

Query: 760  PQEQKTVDAIVKASNLPLSIVLVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMSKK 939
            PQEQKTVDAIV AS  PLSIVLVGVGDGPWDMMKEFDDNIPAR FDNFQFVNFT IM+K 
Sbjct: 258  PQEQKTVDAIVAASKYPLSIVLVGVGDGPWDMMKEFDDNIPARDFDNFQFVNFTNIMAKN 317

Query: 940  LSTSRKETEFALSALMEIPSQYKATMELHILGNARGDVPERFALPPPLYSAASL------ 1101
               +RKETEFALSALMEIP QYKAT+EL++LG  RG  PER ALPPPLY+A SL      
Sbjct: 318  APQTRKETEFALSALMEIPLQYKATLELNLLGRRRGTSPERVALPPPLYNARSLSNASSF 377

Query: 1102 GHSKPSQSTSFKK------------XXXXXXXXXXXXXXXXXXXXXFDNQLCPICLSNSK 1245
             ++KPS   SF+                                  +DN LCPICL+N+K
Sbjct: 378  NYTKPSPEKSFQSSTPSYYGNSRDASTTPSAPSSSYDTGPTGATSAYDNLLCPICLTNNK 437

Query: 1246 DMAFGCGHQTC 1278
            DMAFGCGHQTC
Sbjct: 438  DMAFGCGHQTC 448


>ref|XP_004142779.1| PREDICTED: E3 ubiquitin-protein ligase RGLG2-like [Cucumis sativus]
          Length = 447

 Score =  542 bits (1397), Expect = e-152
 Identities = 274/367 (74%), Positives = 308/367 (83%), Gaps = 11/367 (2%)
 Frame = +1

Query: 211  GHEPQKKQLDRRYSRIADNYNSLDEVTAALANAGLESSNLIVGIDFTKSNEWTGAKSFNR 390
            G+    ++LDRRYSRIADNYNSL+EVT ALA AGLESSNLIVGIDFTKSNEWTGA+SFNR
Sbjct: 57   GNVDNGRRLDRRYSRIADNYNSLEEVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNR 116

Query: 391  QSLHHIGGVLNPYEQAISIIGKTLASFDEDNLIPCYGFGDASTHDQDVFSFY--EERYCN 564
            +SLHHIG   NPY+QAISIIGKTLA+FD+DNLIPC+GFGDASTHDQDVFSF+  EER+CN
Sbjct: 117  RSLHHIGDQPNPYQQAISIIGKTLAAFDDDNLIPCFGFGDASTHDQDVFSFFPEEERFCN 176

Query: 565  GFEEVLSRYKEIVPNLRLAGPTSFAPIIEMAMTIVEQSNGQYHVLVIIADGQVTRSVDTE 744
            GFEEVL+RY+EI P+LRLAGPTSFAP+IEMAMTIVEQS GQYHVL+IIADGQVTRSVDTE
Sbjct: 177  GFEEVLNRYQEIAPHLRLAGPTSFAPVIEMAMTIVEQSGGQYHVLLIIADGQVTRSVDTE 236

Query: 745  RGHLSPQEQKTVDAIVKASNLPLSIVLVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTE 924
            RG LSPQEQKTVDAIV+AS  PLSIVLVGVGDGPWDMM+EFDDNIP+RAFDNFQFVNFTE
Sbjct: 237  RGRLSPQEQKTVDAIVEASKFPLSIVLVGVGDGPWDMMREFDDNIPSRAFDNFQFVNFTE 296

Query: 925  IMSKKLSTSRKETEFALSALMEIPSQYKATMELHILGNARGDVPERFALPPPLYSAASL- 1101
            IMSK + TSRKETEFAL+ALMEIPSQYKAT+EL ILG  +G  P+R ALPPP+Y AASL 
Sbjct: 297  IMSKNIPTSRKETEFALAALMEIPSQYKATIELDILGGRKGVSPQRVALPPPVYGAASLN 356

Query: 1102 -----GHSKPSQSTSFK---KXXXXXXXXXXXXXXXXXXXXXFDNQLCPICLSNSKDMAF 1257
                  +SKP+ S+S++                         +DNQ+CPICL+N KDMAF
Sbjct: 357  SSKSFSNSKPTWSSSYEPSVPSFSVNRNPTPTSTAPPVASSTYDNQVCPICLTNPKDMAF 416

Query: 1258 GCGHQTC 1278
            GCGHQTC
Sbjct: 417  GCGHQTC 423