BLASTX nr result
ID: Bupleurum21_contig00009972
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00009972
(1982 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAN67631.1| hypothetical protein VITISV_035299 [Vitis vinifera] 297 e-171
ref|XP_002270385.1| PREDICTED: long chain acyl-CoA synthetase 4 ... 297 e-170
ref|XP_002297977.1| predicted protein [Populus trichocarpa] gi|2... 292 e-170
gb|ABK96686.1| unknown [Populus trichocarpa x Populus deltoides] 289 e-167
gb|ABA00144.1| ACS-like protein [Gossypium hirsutum] 285 e-166
>emb|CAN67631.1| hypothetical protein VITISV_035299 [Vitis vinifera]
Length = 654
Score = 297 bits (760), Expect(4) = e-171
Identities = 138/176 (78%), Positives = 160/176 (90%)
Frame = -1
Query: 605 KLQNMTKGYKHQEAAPLFDKIIFSKVKEGLGGNIRLILSGSAPVSKSVETFLRVVTCADV 426
K N+ KG K EAAP+ DKI+F KVK+GLGGN+RLILSG+AP+S VE +LRVVTCA V
Sbjct: 345 KQYNLWKGVKTLEAAPICDKIVFGKVKQGLGGNVRLILSGAAPLSTHVEAYLRVVTCAHV 404
Query: 425 LQGYGLTETCGGSFVAKVHEVSMIGAVGPPLPSVDVCLKSVPDMGYDALSSTPRGEMCIR 246
LQGYGLTETC G+FV+ +E+SM+G VGPP+P+VDVCL+SVP+MGYDALSSTPRGE+CIR
Sbjct: 405 LQGYGLTETCAGTFVSLPNEISMLGTVGPPVPNVDVCLESVPEMGYDALSSTPRGEICIR 464
Query: 245 GKTLFSGYFKREDLTKEVMIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYV 78
GKTLFSGY+KREDLTKEV++DGWFHTGD+GEWQPDGSMKIIDRKKNIFKLSQGEYV
Sbjct: 465 GKTLFSGYYKREDLTKEVLVDGWFHTGDVGEWQPDGSMKIIDRKKNIFKLSQGEYV 520
Score = 221 bits (564), Expect(4) = e-171
Identities = 103/155 (66%), Positives = 132/155 (85%)
Frame = -2
Query: 1072 KTLVSFGKITDEQKELAKSFDLAIYSWDEFLLLGTNNQFELPVKKKSDICTVMYTSGTTG 893
KT+VSFGK+T EQ+E + F LAIYSW+EFL LG + +F+LP+ KK+DICT+MYTSGTTG
Sbjct: 167 KTIVSFGKVTPEQREEVEKFGLAIYSWEEFLQLGDDKKFDLPLTKKNDICTIMYTSGTTG 226
Query: 892 DPKGVMITNDSIISLLSGVDHHLGSMNEEFTERDVYLSYLPLAHIFDRVMEEQIILVGAS 713
DPKGVMI+N+SI++L++GV L S+NEE E+DV++SYLPLAHIFDRV+EE I GAS
Sbjct: 227 DPKGVMISNESIVTLIAGVKRLLQSVNEELNEKDVFISYLPLAHIFDRVIEELFISHGAS 286
Query: 712 IGFWQGNIKQLMDDVRTLKPPIFCAVPRVLDKIYS 608
IGFW+G++K L++D+ LKP IFCAVPRVLD+IYS
Sbjct: 287 IGFWRGDVKLLVEDIGELKPTIFCAVPRVLDRIYS 321
Score = 116 bits (291), Expect(4) = e-171
Identities = 50/75 (66%), Positives = 64/75 (85%)
Frame = -1
Query: 1295 QGSPCGIYGPNSTKWVISMQACNADGLYCVLLYDTLSSGAVEYIICHAEISIVFVESTKV 1116
+G CGIYG NS +W++SM+ACNA GLYCV LYDTL +GAVE+IICH+E+SIVFVE K+
Sbjct: 93 EGGRCGIYGANSAEWIMSMEACNAHGLYCVPLYDTLGAGAVEFIICHSELSIVFVEEKKI 152
Query: 1115 SEVMKTFPSVSKYLK 1071
EV+KTFP+ ++YLK
Sbjct: 153 PEVLKTFPNTTEYLK 167
Score = 39.7 bits (91), Expect(4) = e-171
Identities = 17/25 (68%), Positives = 20/25 (80%)
Frame = -3
Query: 75 VENLENIYSRASGIHAIWIYGSSYE 1
VENLENIY S I +IWIYG+S+E
Sbjct: 522 VENLENIYGLVSDIDSIWIYGNSFE 546
>ref|XP_002270385.1| PREDICTED: long chain acyl-CoA synthetase 4 [Vitis vinifera]
gi|297742330|emb|CBI34479.3| unnamed protein product
[Vitis vinifera]
Length = 662
Score = 297 bits (760), Expect(4) = e-170
Identities = 138/176 (78%), Positives = 160/176 (90%)
Frame = -1
Query: 605 KLQNMTKGYKHQEAAPLFDKIIFSKVKEGLGGNIRLILSGSAPVSKSVETFLRVVTCADV 426
K N+ KG K EAAP+ DKI+F KVK+GLGGN+RLILSG+AP+S VE +LRVVTCA V
Sbjct: 354 KQYNLWKGVKTLEAAPICDKIVFGKVKQGLGGNVRLILSGAAPLSTHVEAYLRVVTCAHV 413
Query: 425 LQGYGLTETCGGSFVAKVHEVSMIGAVGPPLPSVDVCLKSVPDMGYDALSSTPRGEMCIR 246
LQGYGLTETC G+FV+ +E+SM+G VGPP+P+VDVCL+SVP+MGYDALSSTPRGE+CIR
Sbjct: 414 LQGYGLTETCAGTFVSLPNEISMLGTVGPPVPNVDVCLESVPEMGYDALSSTPRGEICIR 473
Query: 245 GKTLFSGYFKREDLTKEVMIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYV 78
GKTLFSGY+KREDLTKEV++DGWFHTGD+GEWQPDGSMKIIDRKKNIFKLSQGEYV
Sbjct: 474 GKTLFSGYYKREDLTKEVLVDGWFHTGDVGEWQPDGSMKIIDRKKNIFKLSQGEYV 529
Score = 219 bits (558), Expect(4) = e-170
Identities = 102/155 (65%), Positives = 131/155 (84%)
Frame = -2
Query: 1072 KTLVSFGKITDEQKELAKSFDLAIYSWDEFLLLGTNNQFELPVKKKSDICTVMYTSGTTG 893
KT+VSFGK+T EQ+E + F LAIY W+EFL LG + +F+LP+ KK+DICT+MYTSGTTG
Sbjct: 176 KTIVSFGKVTPEQREEVEKFGLAIYYWEEFLQLGDDKKFDLPLTKKNDICTIMYTSGTTG 235
Query: 892 DPKGVMITNDSIISLLSGVDHHLGSMNEEFTERDVYLSYLPLAHIFDRVMEEQIILVGAS 713
DPKGVMI+N+SI++L++GV L S+NEE E+DV++SYLPLAHIFDRV+EE I GAS
Sbjct: 236 DPKGVMISNESIVTLIAGVKRLLQSVNEELNEKDVFISYLPLAHIFDRVIEELFISHGAS 295
Query: 712 IGFWQGNIKQLMDDVRTLKPPIFCAVPRVLDKIYS 608
IGFW+G++K L++D+ LKP IFCAVPRVLD+IYS
Sbjct: 296 IGFWRGDVKLLVEDIGELKPTIFCAVPRVLDRIYS 330
Score = 116 bits (291), Expect(4) = e-170
Identities = 50/75 (66%), Positives = 64/75 (85%)
Frame = -1
Query: 1295 QGSPCGIYGPNSTKWVISMQACNADGLYCVLLYDTLSSGAVEYIICHAEISIVFVESTKV 1116
+G CGIYG NS +W++SM+ACNA GLYCV LYDTL +GAVE+IICH+E+SIVFVE K+
Sbjct: 102 EGGRCGIYGANSAEWIMSMEACNAHGLYCVPLYDTLGAGAVEFIICHSELSIVFVEEKKI 161
Query: 1115 SEVMKTFPSVSKYLK 1071
EV+KTFP+ ++YLK
Sbjct: 162 PEVLKTFPNTTEYLK 176
Score = 39.7 bits (91), Expect(4) = e-170
Identities = 17/25 (68%), Positives = 20/25 (80%)
Frame = -3
Query: 75 VENLENIYSRASGIHAIWIYGSSYE 1
VENLENIY S I +IWIYG+S+E
Sbjct: 531 VENLENIYGLVSDIDSIWIYGNSFE 555
>ref|XP_002297977.1| predicted protein [Populus trichocarpa] gi|222845235|gb|EEE82782.1|
predicted protein [Populus trichocarpa]
Length = 662
Score = 292 bits (748), Expect(4) = e-170
Identities = 135/176 (76%), Positives = 160/176 (90%)
Frame = -1
Query: 605 KLQNMTKGYKHQEAAPLFDKIIFSKVKEGLGGNIRLILSGSAPVSKSVETFLRVVTCADV 426
K +M KG+ H EA+P+ DKI+F+KV++GLGGN+RLILSG+AP+S VE FLRVV+CA V
Sbjct: 354 KFSSMKKGFAHDEASPICDKIVFNKVRQGLGGNVRLILSGAAPLSNHVEAFLRVVSCAHV 413
Query: 425 LQGYGLTETCGGSFVAKVHEVSMIGAVGPPLPSVDVCLKSVPDMGYDALSSTPRGEMCIR 246
LQGYGLTETC G+FV+ +E+ M+G VGPP+P+VDV L+SVP+MGYDALSSTPRGE+CIR
Sbjct: 414 LQGYGLTETCAGTFVSLPNELPMLGTVGPPVPNVDVFLESVPEMGYDALSSTPRGEICIR 473
Query: 245 GKTLFSGYFKREDLTKEVMIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYV 78
GKTLF+GY+KREDLTKEV+IDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYV
Sbjct: 474 GKTLFAGYYKREDLTKEVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYV 529
Score = 228 bits (580), Expect(4) = e-170
Identities = 107/155 (69%), Positives = 130/155 (83%)
Frame = -2
Query: 1072 KTLVSFGKITDEQKELAKSFDLAIYSWDEFLLLGTNNQFELPVKKKSDICTVMYTSGTTG 893
KT+VSFGK+ ++KE + F LA+YSWDEFL LG N Q+ELPVKKK DICT+MYTSGTTG
Sbjct: 176 KTIVSFGKVASKEKEEIEKFGLAVYSWDEFLNLGENKQYELPVKKKEDICTIMYTSGTTG 235
Query: 892 DPKGVMITNDSIISLLSGVDHHLGSMNEEFTERDVYLSYLPLAHIFDRVMEEQIILVGAS 713
DPKGVMI+NDSI++LL+GV L S+NE T +DVYLSYLPLAHIFDRV+EE I GAS
Sbjct: 236 DPKGVMISNDSIVTLLAGVKRLLESVNEALTSKDVYLSYLPLAHIFDRVIEELFIQHGAS 295
Query: 712 IGFWQGNIKQLMDDVRTLKPPIFCAVPRVLDKIYS 608
IGFW+G++K L++D+ LKP IFCAVPRVLD++YS
Sbjct: 296 IGFWRGDVKLLLEDIGELKPSIFCAVPRVLDRVYS 330
Score = 109 bits (272), Expect(4) = e-170
Identities = 46/74 (62%), Positives = 62/74 (83%)
Frame = -1
Query: 1292 GSPCGIYGPNSTKWVISMQACNADGLYCVLLYDTLSSGAVEYIICHAEISIVFVESTKVS 1113
G+ CGIYG N +WV+SM+ACNA GLYCV LYDTL + AVE+IICH+E+SI FVE K++
Sbjct: 103 GAKCGIYGANCAEWVMSMEACNAHGLYCVPLYDTLGADAVEFIICHSEVSIAFVEEKKIT 162
Query: 1112 EVMKTFPSVSKYLK 1071
E++KTFP+ ++Y+K
Sbjct: 163 ELLKTFPNSTQYMK 176
Score = 40.8 bits (94), Expect(4) = e-170
Identities = 17/25 (68%), Positives = 21/25 (84%)
Frame = -3
Query: 75 VENLENIYSRASGIHAIWIYGSSYE 1
VENLENIYS S I +IW+YG+S+E
Sbjct: 531 VENLENIYSLVSDIDSIWVYGNSFE 555
>gb|ABK96686.1| unknown [Populus trichocarpa x Populus deltoides]
Length = 662
Score = 289 bits (739), Expect(4) = e-167
Identities = 133/176 (75%), Positives = 159/176 (90%)
Frame = -1
Query: 605 KLQNMTKGYKHQEAAPLFDKIIFSKVKEGLGGNIRLILSGSAPVSKSVETFLRVVTCADV 426
K ++M G+ H EA+P+ DK +F+KV++GLGGN+RLILSG+AP+S VE FLRVV+CA V
Sbjct: 354 KFRSMKMGFAHDEASPICDKFVFNKVRQGLGGNVRLILSGAAPLSNHVEAFLRVVSCAHV 413
Query: 425 LQGYGLTETCGGSFVAKVHEVSMIGAVGPPLPSVDVCLKSVPDMGYDALSSTPRGEMCIR 246
LQGYGLTETC G+FV+ +E+ M+G VGPP+P+VDV L+SVP+MGYDALSSTPRGE+CIR
Sbjct: 414 LQGYGLTETCAGTFVSLPNELPMLGTVGPPVPNVDVFLESVPEMGYDALSSTPRGEICIR 473
Query: 245 GKTLFSGYFKREDLTKEVMIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYV 78
GKTLF+GY+KREDLTKEV+IDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYV
Sbjct: 474 GKTLFAGYYKREDLTKEVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYV 529
Score = 223 bits (568), Expect(4) = e-167
Identities = 105/155 (67%), Positives = 129/155 (83%)
Frame = -2
Query: 1072 KTLVSFGKITDEQKELAKSFDLAIYSWDEFLLLGTNNQFELPVKKKSDICTVMYTSGTTG 893
KT+VSFGK+ ++KE + F LA+YSWDEFL LG N Q+ELPVKKK DICT+MYTSGTTG
Sbjct: 176 KTIVSFGKVASKEKEEIEKFGLAVYSWDEFLKLGENKQYELPVKKKEDICTIMYTSGTTG 235
Query: 892 DPKGVMITNDSIISLLSGVDHHLGSMNEEFTERDVYLSYLPLAHIFDRVMEEQIILVGAS 713
PKGVMI+NDSI++LL+GV L S+NE T +DVYLSYLPLAHIFDRV+EE I GAS
Sbjct: 236 YPKGVMISNDSIVTLLAGVKRLLESVNEALTSKDVYLSYLPLAHIFDRVIEELFIQHGAS 295
Query: 712 IGFWQGNIKQLMDDVRTLKPPIFCAVPRVLDKIYS 608
IGFW+G++K L++D+ LKP IFCAVPRVL+++YS
Sbjct: 296 IGFWRGDVKLLLEDIGELKPSIFCAVPRVLERVYS 330
Score = 109 bits (272), Expect(4) = e-167
Identities = 46/74 (62%), Positives = 62/74 (83%)
Frame = -1
Query: 1292 GSPCGIYGPNSTKWVISMQACNADGLYCVLLYDTLSSGAVEYIICHAEISIVFVESTKVS 1113
G+ CGIYG N +WV+SM+ACNA GLYCV LYDTL + AVE+IICH+E+SI FVE K++
Sbjct: 103 GAKCGIYGANCAEWVMSMEACNAHGLYCVPLYDTLGADAVEFIICHSEVSIAFVEEKKIT 162
Query: 1112 EVMKTFPSVSKYLK 1071
E++KTFP+ ++Y+K
Sbjct: 163 ELLKTFPNSTQYMK 176
Score = 40.8 bits (94), Expect(4) = e-167
Identities = 17/25 (68%), Positives = 21/25 (84%)
Frame = -3
Query: 75 VENLENIYSRASGIHAIWIYGSSYE 1
VENLENIYS S I +IW+YG+S+E
Sbjct: 531 VENLENIYSLVSDIDSIWVYGNSFE 555
>gb|ABA00144.1| ACS-like protein [Gossypium hirsutum]
Length = 662
Score = 285 bits (729), Expect(4) = e-166
Identities = 133/176 (75%), Positives = 157/176 (89%)
Frame = -1
Query: 605 KLQNMTKGYKHQEAAPLFDKIIFSKVKEGLGGNIRLILSGSAPVSKSVETFLRVVTCADV 426
K NM KG KH EA+P+ DKI+FSKVK+GLGGN+RLILSG+AP+S VE FLRVV C V
Sbjct: 354 KYYNMKKGRKHGEASPICDKIVFSKVKQGLGGNVRLILSGAAPLSTHVEEFLRVVACCHV 413
Query: 425 LQGYGLTETCGGSFVAKVHEVSMIGAVGPPLPSVDVCLKSVPDMGYDALSSTPRGEMCIR 246
LQGYGLTE+C GSFV+ +E+SM+G VGPP+P++DV L+SVP+M YDAL+STPRGE+CI+
Sbjct: 414 LQGYGLTESCAGSFVSLPNELSMLGTVGPPVPNIDVRLESVPEMNYDALASTPRGEICIK 473
Query: 245 GKTLFSGYFKREDLTKEVMIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGEYV 78
G TLFSGY+KREDLT+EV+IDGWFHTGDIGEWQP+GSMKIIDRKKNIFKLSQGEYV
Sbjct: 474 GNTLFSGYYKREDLTREVLIDGWFHTGDIGEWQPNGSMKIIDRKKNIFKLSQGEYV 529
Score = 220 bits (560), Expect(4) = e-166
Identities = 104/155 (67%), Positives = 128/155 (82%)
Frame = -2
Query: 1072 KTLVSFGKITDEQKELAKSFDLAIYSWDEFLLLGTNNQFELPVKKKSDICTVMYTSGTTG 893
KT+VSFGK+T EQK A+ L IY W+EFL LG N + LPVKKK+DICT+MYTSGTTG
Sbjct: 176 KTIVSFGKVTPEQKAEAEKHGLKIYPWEEFLQLGENKNYGLPVKKKTDICTIMYTSGTTG 235
Query: 892 DPKGVMITNDSIISLLSGVDHHLGSMNEEFTERDVYLSYLPLAHIFDRVMEEQIILVGAS 713
DPKGV I+NDSI++L++GV LGS+NE+ T +DVY+SYLPLAHIFDRV+EE I GAS
Sbjct: 236 DPKGVFISNDSIVTLIAGVKRLLGSVNEQLTMKDVYISYLPLAHIFDRVIEELFISHGAS 295
Query: 712 IGFWQGNIKQLMDDVRTLKPPIFCAVPRVLDKIYS 608
IGFW+G++K L++D+ LKP IFCAVPRVLD+IYS
Sbjct: 296 IGFWRGDVKLLVEDIGELKPSIFCAVPRVLDRIYS 330
Score = 112 bits (280), Expect(4) = e-166
Identities = 49/75 (65%), Positives = 62/75 (82%)
Frame = -1
Query: 1295 QGSPCGIYGPNSTKWVISMQACNADGLYCVLLYDTLSSGAVEYIICHAEISIVFVESTKV 1116
+G CGIYG N +W+ISM+ACNA GLYCV LYDTL +GAVE+IICHAEISI FVE K+
Sbjct: 102 EGGKCGIYGANCPEWIISMEACNAHGLYCVPLYDTLGAGAVEFIICHAEISIAFVEEKKI 161
Query: 1115 SEVMKTFPSVSKYLK 1071
+E+ KTFP+ +++LK
Sbjct: 162 NELFKTFPASTEHLK 176
Score = 40.0 bits (92), Expect(4) = e-166
Identities = 17/25 (68%), Positives = 20/25 (80%)
Frame = -3
Query: 75 VENLENIYSRASGIHAIWIYGSSYE 1
VENLENIY S I +IWIYG+S+E
Sbjct: 531 VENLENIYGLVSAIDSIWIYGNSFE 555