BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00009885
         (1002 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFW16645.1| putative progesterone 5beta-reductase [Bupleurum ...   609   e-172
gb|ADG41743.1| putative progesterone 5 beta-reductase [Nicotiana...   486   e-135
gb|AFZ41795.1| putative progesterone 5-beta-reductase [Duboisia ...   485   e-135
dbj|BAH47641.1| progesterone 5beta reductase-B [Nicotiana tabacum]    483   e-134
gb|AEX31545.1| putative steroid 5beta-reductase [Solanum lycoper...   478   e-133

>gb|AFW16645.1| putative progesterone 5beta-reductase [Bupleurum falcatum]
          Length = 388

 Score =  609 bits (1570), Expect = e-172
 Identities = 284/288 (98%), Positives = 287/288 (99%)
 Frame = -2

Query: 866 MSWWWSGAVGAARKTIDQENVELKYESVALVVGVTGIVGNSLAEILPRSDTPGSPWKVYG 687
           MSWWWSGAVGAARKTIDQENVELKYESVALVVGVTGIVGNSLAEILPR+DTPGSPWKVYG
Sbjct: 1   MSWWWSGAVGAARKTIDQENVELKYESVALVVGVTGIVGNSLAEILPRTDTPGSPWKVYG 60

Query: 686 IARRPRPQWDANHPVEYIQCDISNPEETESRLSHLKDVTHLFYVTWASRPTEAENCEINS 507
           IARRPRPQWDANHPVEYIQCDISNPEETESRLSHLKDVTHLFYVTWASRPTEAENCEINS
Sbjct: 61  IARRPRPQWDANHPVEYIQCDISNPEETESRLSHLKDVTHLFYVTWASRPTEAENCEINS 120

Query: 506 QMFKNLLNCIIPNAPKLQHICLQTGKKHYFGSFDSYGGVAHDPPYSEDLPRLNAPNFYYN 327
           QMF+NLLNCIIPNAPKLQHICLQTGKKHY GSFDSYGGVAHDPPYSEDLPRLNAPNFYYN
Sbjct: 121 QMFRNLLNCIIPNAPKLQHICLQTGKKHYLGSFDSYGGVAHDPPYSEDLPRLNAPNFYYN 180

Query: 326 LEDILFEEVKKKKGLTWSVHRPGTIFGFSPNSMMNIICTLCVYASICKHEGVAMRFPGTK 147
           LEDILFEEV+KKKGLTWSVHRPGTIFGFSPNSMMNIICTLCVYASICKHEGVAMRFPGTK
Sbjct: 181 LEDILFEEVEKKKGLTWSVHRPGTIFGFSPNSMMNIICTLCVYASICKHEGVAMRFPGTK 240

Query: 146 EAWSSYSEASDADLIAEQEIWAVVDPYAKNEAFNCSNGDVFKWKHFWK 3
           EAWSSYSEASDADLIAEQEIWAVVDPYAKNEAFNCSNGDVFKWKHFWK
Sbjct: 241 EAWSSYSEASDADLIAEQEIWAVVDPYAKNEAFNCSNGDVFKWKHFWK 288


>gb|ADG41743.1| putative progesterone 5 beta-reductase [Nicotiana tabacum]
          Length = 388

 Score =  486 bits (1252), Expect = e-135
 Identities = 213/288 (73%), Positives = 254/288 (88%)
 Frame = -2

Query: 866 MSWWWSGAVGAARKTIDQENVELKYESVALVVGVTGIVGNSLAEILPRSDTPGSPWKVYG 687
           MSWWW+GA+GAA+K  ++++   KY+SVAL++GVTGIVGNSLAEILP +DTPG PWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDDAPPKYQSVALIIGVTGIVGNSLAEILPLADTPGGPWKVYG 60

Query: 686 IARRPRPQWDANHPVEYIQCDISNPEETESRLSHLKDVTHLFYVTWASRPTEAENCEINS 507
           +ARRPRP W+A+HP++YIQCDISNPE+T+S+LS L DVTH+FYVTWASR TE ENCEIN 
Sbjct: 61  VARRPRPSWNADHPIDYIQCDISNPEDTQSKLSLLTDVTHVFYVTWASRSTEVENCEING 120

Query: 506 QMFKNLLNCIIPNAPKLQHICLQTGKKHYFGSFDSYGGVAHDPPYSEDLPRLNAPNFYYN 327
           +MF+N+L+ IIPN P L+HICLQTG+KHY G F+ YG VAHD P+ EDLPRL+APNFYY 
Sbjct: 121 KMFQNVLSVIIPNCPNLRHICLQTGRKHYLGPFELYGKVAHDSPFHEDLPRLDAPNFYYT 180

Query: 326 LEDILFEEVKKKKGLTWSVHRPGTIFGFSPNSMMNIICTLCVYASICKHEGVAMRFPGTK 147
           LED+LF+EV+KK+GLTWSVHRPGTIFGFSP S+MNI+ TLCVYA+ICKHEG+ ++FPG K
Sbjct: 181 LEDVLFKEVEKKEGLTWSVHRPGTIFGFSPYSLMNIVGTLCVYAAICKHEGLPLKFPGVK 240

Query: 146 EAWSSYSEASDADLIAEQEIWAVVDPYAKNEAFNCSNGDVFKWKHFWK 3
            AW  YS+ SDADLIAE +IWA VDPYAKNEAFN SNGDVFKWKHFWK
Sbjct: 241 AAWDGYSDCSDADLIAEHQIWAAVDPYAKNEAFNVSNGDVFKWKHFWK 288


>gb|AFZ41795.1| putative progesterone 5-beta-reductase [Duboisia hopwoodii]
          Length = 388

 Score =  485 bits (1249), Expect = e-135
 Identities = 214/288 (74%), Positives = 253/288 (87%)
 Frame = -2

Query: 866 MSWWWSGAVGAARKTIDQENVELKYESVALVVGVTGIVGNSLAEILPRSDTPGSPWKVYG 687
           MSWWW+GA+GAA+K  ++++   KYESVAL++GVTGIVGNSLAEILP +DTPG PWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDDAPPKYESVALIIGVTGIVGNSLAEILPLADTPGGPWKVYG 60

Query: 686 IARRPRPQWDANHPVEYIQCDISNPEETESRLSHLKDVTHLFYVTWASRPTEAENCEINS 507
           +ARRPRP W+A+HP+EYIQCDISN E+T+S+LS L DVTH+FYVTWASR TE ENCEIN 
Sbjct: 61  VARRPRPSWNADHPIEYIQCDISNTEDTQSKLSVLTDVTHVFYVTWASRSTEVENCEING 120

Query: 506 QMFKNLLNCIIPNAPKLQHICLQTGKKHYFGSFDSYGGVAHDPPYSEDLPRLNAPNFYYN 327
           +MF+N+L+ IIPN P L+HICLQTG+KHY G F+ YG VAHD P+ EDLPRL+APNFYY 
Sbjct: 121 KMFQNVLSVIIPNCPNLRHICLQTGRKHYLGPFELYGKVAHDSPFHEDLPRLDAPNFYYT 180

Query: 326 LEDILFEEVKKKKGLTWSVHRPGTIFGFSPNSMMNIICTLCVYASICKHEGVAMRFPGTK 147
           LED+LF+EV+KK+GLTWSVHRPGTIFGFSP S+MNI+ TLCVYA+ICKHEG+ ++FPG K
Sbjct: 181 LEDVLFKEVEKKEGLTWSVHRPGTIFGFSPFSLMNIVGTLCVYAAICKHEGLPLKFPGVK 240

Query: 146 EAWSSYSEASDADLIAEQEIWAVVDPYAKNEAFNCSNGDVFKWKHFWK 3
            AW  YS+ SDADLIAE +IWA VDPYAKNEAFN SNGDVFKWKHFWK
Sbjct: 241 AAWDGYSDCSDADLIAEHQIWAAVDPYAKNEAFNVSNGDVFKWKHFWK 288


>dbj|BAH47641.1| progesterone 5beta reductase-B [Nicotiana tabacum]
          Length = 388

 Score =  483 bits (1244), Expect = e-134
 Identities = 212/288 (73%), Positives = 253/288 (87%)
 Frame = -2

Query: 866 MSWWWSGAVGAARKTIDQENVELKYESVALVVGVTGIVGNSLAEILPRSDTPGSPWKVYG 687
           MSWWW+GA+GAA+K  ++++   KY+SVAL++GVTGIVGNSLAEILP +DTPG PWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDDAPPKYQSVALIIGVTGIVGNSLAEILPLADTPGGPWKVYG 60

Query: 686 IARRPRPQWDANHPVEYIQCDISNPEETESRLSHLKDVTHLFYVTWASRPTEAENCEINS 507
           +ARRPRP W+A+HP++YIQCDISNPE+T+S+LS L DVTH+FYVTWASR TE ENCEIN 
Sbjct: 61  VARRPRPSWNADHPIDYIQCDISNPEDTQSKLSLLTDVTHVFYVTWASRSTEVENCEING 120

Query: 506 QMFKNLLNCIIPNAPKLQHICLQTGKKHYFGSFDSYGGVAHDPPYSEDLPRLNAPNFYYN 327
           +MF+N+L+ IIPN P L+HICLQTG+KHY G F+ YG VAHD P+ EDLPRL+APNFYY 
Sbjct: 121 KMFQNVLSVIIPNCPNLRHICLQTGRKHYLGPFELYGKVAHDFPFHEDLPRLDAPNFYYT 180

Query: 326 LEDILFEEVKKKKGLTWSVHRPGTIFGFSPNSMMNIICTLCVYASICKHEGVAMRFPGTK 147
           LED+LF+EV+KK+GLTWSVHRPGTIFGFSP S+MNI+ TLCVYA+ICKHEG+ ++FPG K
Sbjct: 181 LEDVLFKEVEKKEGLTWSVHRPGTIFGFSPYSLMNIVGTLCVYAAICKHEGLPLKFPGVK 240

Query: 146 EAWSSYSEASDADLIAEQEIWAVVDPYAKNEAFNCSNGDVFKWKHFWK 3
            AW  YS+ SDADLIAE +IWA VDPYAKNEAFN  NGDVFKWKHFWK
Sbjct: 241 AAWDGYSDCSDADLIAEHQIWAAVDPYAKNEAFNVRNGDVFKWKHFWK 288


>gb|AEX31545.1| putative steroid 5beta-reductase [Solanum lycopersicum]
          Length = 387

 Score =  478 bits (1231), Expect = e-133
 Identities = 211/285 (74%), Positives = 249/285 (87%)
 Frame = -2

Query: 857 WWSGAVGAARKTIDQENVELKYESVALVVGVTGIVGNSLAEILPRSDTPGSPWKVYGIAR 678
           WW+GA+GAA+K  + +    KYESVAL++GVTGIVGNSLAEILP +DTPG PWKVYG+AR
Sbjct: 3   WWAGAIGAAKKKFEDDEAPPKYESVALIIGVTGIVGNSLAEILPLADTPGGPWKVYGVAR 62

Query: 677 RPRPQWDANHPVEYIQCDISNPEETESRLSHLKDVTHLFYVTWASRPTEAENCEINSQMF 498
           R RP W+A+HP+EY+QCDISNPE+T+S+LS L DVTH+FYVTWA+R TE ENCEIN +MF
Sbjct: 63  RARPSWNADHPIEYVQCDISNPEDTQSKLSVLTDVTHVFYVTWANRSTEVENCEINGKMF 122

Query: 497 KNLLNCIIPNAPKLQHICLQTGKKHYFGSFDSYGGVAHDPPYSEDLPRLNAPNFYYNLED 318
           +N+LN IIPN P L+HICLQTG+KHY G F+ YG V+HDPP+ EDLPRL+APNFYY LED
Sbjct: 123 RNVLNVIIPNCPNLRHICLQTGRKHYLGPFELYGKVSHDPPFHEDLPRLDAPNFYYVLED 182

Query: 317 ILFEEVKKKKGLTWSVHRPGTIFGFSPNSMMNIICTLCVYASICKHEGVAMRFPGTKEAW 138
           ILF+EV+KK+GLTWSVHRPGTIFGFSP S+MNI+ TLCVYA+ICKHEG+ ++FPG K AW
Sbjct: 183 ILFKEVEKKEGLTWSVHRPGTIFGFSPYSLMNIVGTLCVYAAICKHEGLPLKFPGVKGAW 242

Query: 137 SSYSEASDADLIAEQEIWAVVDPYAKNEAFNCSNGDVFKWKHFWK 3
             YS+ SDADLIAE +IWA VDPYAKNEAFN SNGDVFKWKHFWK
Sbjct: 243 DGYSDCSDADLIAEHQIWAAVDPYAKNEAFNVSNGDVFKWKHFWK 287