BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00009803
         (1908 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002270121.1| PREDICTED: uncharacterized calcium-binding p...   668   0.0  
emb|CAN73791.1| hypothetical protein VITISV_034892 [Vitis vinifera]   667   0.0  
ref|XP_004137201.1| PREDICTED: uncharacterized calcium-binding p...   667   0.0  
ref|XP_002317830.1| predicted protein [Populus trichocarpa] gi|2...   652   0.0  
ref|NP_001078754.1| calcium-binding endonuclease/exonuclease/pho...   640   0.0  

>ref|XP_002270121.1| PREDICTED: uncharacterized calcium-binding protein At1g02270 [Vitis
            vinifera] gi|302144002|emb|CBI23107.3| unnamed protein
            product [Vitis vinifera]
          Length = 451

 Score =  668 bits (1724), Expect = 0.0
 Identities = 322/454 (70%), Positives = 377/454 (83%), Gaps = 7/454 (1%)
 Frame = +1

Query: 64   MGRKQRERDGLGRGRLPRIQSYAIASDPSS------FSCTTFNILAPIYKRLDHQDQSCR 225
            M R++R +    +GR+ RI +YAIAS           SCTTFNILAPIYKRL+H+D +CR
Sbjct: 1    MAREKRMK----KGRVSRIGNYAIASSLRDHHHHPCISCTTFNILAPIYKRLNHEDTNCR 56

Query: 226  ESDNKDNWVNRNQRILDMLLYERSSIICLQEFWVGNNELVELYDKRLGDAGYITFKLART 405
            ESD +  W++RN+ ILD L+ ERSSIICLQEFWVGN ELV LY+KRLGDAGYI FKLART
Sbjct: 57   ESDCRTYWLSRNRAILDWLIDERSSIICLQEFWVGNEELVNLYEKRLGDAGYINFKLART 116

Query: 406  NNRGDGLLTAVHKDYFRVINTEPVLLHGFGDRVAQLLHVEIATPFSQCRNNSVRQELLIV 585
            NNRGDGLLT+VHKDYFRV+N   +L + FGDRVAQLLHVE+  PF  CR+++  QE+LIV
Sbjct: 117  NNRGDGLLTSVHKDYFRVLNHRALLFNDFGDRVAQLLHVELVAPFPHCRSSNTHQEILIV 176

Query: 586  NTHLLFPHDSSLCLERLRQVYKILQHVECYQKENKLHSLPILLCGDWNGSKRGHVYKFLR 765
            NTHLLFPHDS+LC+ RL+QVYKILQ+VE YQKENKL+ +PI+LCGDWNGSK GHVYKFLR
Sbjct: 177  NTHLLFPHDSTLCIIRLQQVYKILQYVESYQKENKLNPMPIILCGDWNGSKSGHVYKFLR 236

Query: 766  SQGFISSYDTAHEYTDTDAQKWVSHRNHRGNICGVDFIWLLNPSSYRKVLKVSWSETVFG 945
            SQGF+SSYDTAH+YTD D  KWVSHRNHRGNICGVDFIWLLNP+ YRK+LK SWSE VFG
Sbjct: 237  SQGFVSSYDTAHQYTDADEHKWVSHRNHRGNICGVDFIWLLNPNRYRKLLKTSWSEAVFG 296

Query: 946  MFKCLLRRASLTADDAFAILKADSSGDFITYPGFCDALRQLNVTGEQYGLSMDETKDLWE 1125
            MFK LLRRASLT DDAFA LKAD+ GD ITY GFC+ALRQLN+TG  +GLS +E  DLW 
Sbjct: 297  MFKYLLRRASLTEDDAFAFLKADNHGDCITYSGFCEALRQLNLTGHCHGLSDEEINDLWV 356

Query: 1126 QADTDGNGIVDYKEFQLRIWNPTWSDQRFDINNEIREDIVNA-TEQTVGYTVKNAVLFPN 1302
            QAD DGNG++DYKEFQ RIWNPTWS QR +  NE  ++++    EQ +G++VKNAVLFP+
Sbjct: 357  QADIDGNGVLDYKEFQKRIWNPTWSQQRDENINEAWDEVMKGPKEQAIGFSVKNAVLFPS 416

Query: 1303 EVEKGMWPEDYSLSDHARLTVVFSPVKMLCSRPT 1404
            EVEKGMWPE+YSLSDHARLTVVFSPV+M C++ T
Sbjct: 417  EVEKGMWPENYSLSDHARLTVVFSPVRMPCAQIT 450


>emb|CAN73791.1| hypothetical protein VITISV_034892 [Vitis vinifera]
          Length = 445

 Score =  667 bits (1722), Expect = 0.0
 Identities = 319/442 (72%), Positives = 371/442 (83%), Gaps = 7/442 (1%)
 Frame = +1

Query: 100  RGRLPRIQSYAIASDPSS------FSCTTFNILAPIYKRLDHQDQSCRESDNKDNWVNRN 261
            +GR+ RI +YAIAS           SCTTFNILAPIYKRL+H+D +CRESD +  W++RN
Sbjct: 3    KGRVSRIGNYAIASSLRDHHHHPCISCTTFNILAPIYKRLNHEDTNCRESDCRTYWLSRN 62

Query: 262  QRILDMLLYERSSIICLQEFWVGNNELVELYDKRLGDAGYITFKLARTNNRGDGLLTAVH 441
            + ILD L+ ERSSIICLQEFWVGN ELV LY+KRLGDAGYI FKLARTNNRGDGLLT+VH
Sbjct: 63   RAILDWLIDERSSIICLQEFWVGNEELVNLYEKRLGDAGYINFKLARTNNRGDGLLTSVH 122

Query: 442  KDYFRVINTEPVLLHGFGDRVAQLLHVEIATPFSQCRNNSVRQELLIVNTHLLFPHDSSL 621
            KDYFRV+N   +L + FGDRVAQLLHVE+  PF  CR+++  QE+LIVNTHLLFPHDS+L
Sbjct: 123  KDYFRVLNHRALLFNDFGDRVAQLLHVELVAPFPHCRSSNTHQEILIVNTHLLFPHDSTL 182

Query: 622  CLERLRQVYKILQHVECYQKENKLHSLPILLCGDWNGSKRGHVYKFLRSQGFISSYDTAH 801
            C+ RL+QVYKILQ+VE YQKENKL+ +PI+LCGDWNGSK GHVYKFLRSQGF+SSYDTAH
Sbjct: 183  CIIRLQQVYKILQYVESYQKENKLNPMPIILCGDWNGSKSGHVYKFLRSQGFVSSYDTAH 242

Query: 802  EYTDTDAQKWVSHRNHRGNICGVDFIWLLNPSSYRKVLKVSWSETVFGMFKCLLRRASLT 981
            +YTD D  KWVSHRNHRGNICGVDFIWLLNP+ YRK+LK SWSE VFGMFK LLRRASLT
Sbjct: 243  QYTDADEHKWVSHRNHRGNICGVDFIWLLNPNRYRKLLKTSWSEAVFGMFKYLLRRASLT 302

Query: 982  ADDAFAILKADSSGDFITYPGFCDALRQLNVTGEQYGLSMDETKDLWEQADTDGNGIVDY 1161
             DDAFA LKAD+ GD ITY GFC+ALRQLN+TG  +GLS +E  DLW QAD DGNG++DY
Sbjct: 303  EDDAFAFLKADNHGDCITYSGFCEALRQLNLTGHCHGLSDEEINDLWVQADIDGNGVLDY 362

Query: 1162 KEFQLRIWNPTWSDQRFDINNEIREDIVNA-TEQTVGYTVKNAVLFPNEVEKGMWPEDYS 1338
            KEFQ RIWNPTWS QR +  NE  ++++    EQ +G++VKNAVLFP+EVEKGMWPE+YS
Sbjct: 363  KEFQKRIWNPTWSQQRDENINEAWDEVMKGPKEQAIGFSVKNAVLFPSEVEKGMWPENYS 422

Query: 1339 LSDHARLTVVFSPVKMLCSRPT 1404
            LSDHARLTVVFSPV+M C++ T
Sbjct: 423  LSDHARLTVVFSPVRMPCAQIT 444


>ref|XP_004137201.1| PREDICTED: uncharacterized calcium-binding protein At1g02270-like
            [Cucumis sativus] gi|449483236|ref|XP_004156530.1|
            PREDICTED: uncharacterized calcium-binding protein
            At1g02270-like [Cucumis sativus]
          Length = 447

 Score =  667 bits (1720), Expect = 0.0
 Identities = 321/445 (72%), Positives = 373/445 (83%), Gaps = 7/445 (1%)
 Frame = +1

Query: 85   RDGLGRGRLPRIQSYAIASDPS-------SFSCTTFNILAPIYKRLDHQDQSCRESDNKD 243
            RD   +GR+  I SYAIAS          S +CTTFNILAPIY RL+ QD SCRESD + 
Sbjct: 3    RDKKMKGRISMIGSYAIASSIKDHHHQRPSITCTTFNILAPIYNRLNQQDPSCRESDYRT 62

Query: 244  NWVNRNQRILDMLLYERSSIICLQEFWVGNNELVELYDKRLGDAGYITFKLARTNNRGDG 423
             W+ RNQRILD LLYE+SSIICLQEFWVGN ELV +Y+ RLG+AGYI+FKLARTNNRGDG
Sbjct: 63   YWLARNQRILDWLLYEKSSIICLQEFWVGNQELVNIYENRLGNAGYISFKLARTNNRGDG 122

Query: 424  LLTAVHKDYFRVINTEPVLLHGFGDRVAQLLHVEIATPFSQCRNNSVRQELLIVNTHLLF 603
            LLTAVHKDYFRV+N   +L +  GDRVAQLLHVE+A PFS CRNN +RQE+LIVNTHLLF
Sbjct: 123  LLTAVHKDYFRVVNYRELLFNDCGDRVAQLLHVELAVPFSHCRNNDIRQEILIVNTHLLF 182

Query: 604  PHDSSLCLERLRQVYKILQHVECYQKENKLHSLPILLCGDWNGSKRGHVYKFLRSQGFIS 783
            PHDSSLCL RL QVYKILQ+VE YQKENKL+ +PI+LCGDWNGSKRGHVYKFLRSQGF+S
Sbjct: 183  PHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQGFVS 242

Query: 784  SYDTAHEYTDTDAQKWVSHRNHRGNICGVDFIWLLNPSSYRKVLKVSWSETVFGMFKCLL 963
            SYDTAH+YTD D++KWVSHRNHRGNICGVDFIWLLNP+ YR++LK SWSE +FGMFK LL
Sbjct: 243  SYDTAHQYTDADSRKWVSHRNHRGNICGVDFIWLLNPNGYRRLLKASWSEAIFGMFKYLL 302

Query: 964  RRASLTADDAFAILKADSSGDFITYPGFCDALRQLNVTGEQYGLSMDETKDLWEQADTDG 1143
            RRASLTADDAFA LKAD+ GD+ITY GFC+ LRQLN+TG  +GLS++E  DLW QAD+DG
Sbjct: 303  RRASLTADDAFAFLKADNDGDYITYSGFCEGLRQLNLTGHLHGLSVEEINDLWVQADSDG 362

Query: 1144 NGIVDYKEFQLRIWNPTWSDQRFDINNEIREDIVNATEQTVGYTVKNAVLFPNEVEKGMW 1323
            NGI+D+ EFQ RIWN T +++R + +NEI        EQT+G++V+NAVLFP EVEKG W
Sbjct: 363  NGILDHNEFQ-RIWNSTGTEKRDEKSNEIESK--ENQEQTIGFSVENAVLFPAEVEKGRW 419

Query: 1324 PEDYSLSDHARLTVVFSPVKMLCSR 1398
            PEDYSLSDHARLTVVF+P++M CS+
Sbjct: 420  PEDYSLSDHARLTVVFAPIRMPCSQ 444


>ref|XP_002317830.1| predicted protein [Populus trichocarpa] gi|222858503|gb|EEE96050.1|
            predicted protein [Populus trichocarpa]
          Length = 451

 Score =  652 bits (1681), Expect = 0.0
 Identities = 326/457 (71%), Positives = 371/457 (81%), Gaps = 12/457 (2%)
 Frame = +1

Query: 64   MGRKQRERDGLGRGRLPRIQSYAIASDPSS--------FSCTTFNILAPIYKRLD---HQ 210
            M R++R R      R+ RI SYAI+S  S          +CTTFNILAPIYKRL+    +
Sbjct: 1    MTRERRSR------RISRIGSYAISSSMSMRDHRQQPCITCTTFNILAPIYKRLNINNDK 54

Query: 211  DQSCRESDNKDNWVNRNQRILDMLLYERSSIICLQEFWVGNNELVELYDKRLGDAGYITF 390
            DQ+ RESD +  W+ RNQRILD LL ERSSIICLQEFW+GN ELV +Y+KRLGDAGY+ F
Sbjct: 55   DQNSRESDYRAYWLVRNQRILDSLLRERSSIICLQEFWLGNEELVNMYEKRLGDAGYLNF 114

Query: 391  KLARTNNRGDGLLTAVHKDYFRVINTEPVLLHGFGDRVAQLLHVEIATPFSQCRNNSVRQ 570
            KLARTNNRGDGLL AV KDYFRVIN   +L +  GDRVAQLLHVE+A P S CRNN  RQ
Sbjct: 115  KLARTNNRGDGLLIAVRKDYFRVINHRELLFNDCGDRVAQLLHVELAAPCSPCRNNDTRQ 174

Query: 571  ELLIVNTHLLFPHDSSLCLERLRQVYKILQHVECYQKENKLHSLPILLCGDWNGSKRGHV 750
            E+LIVNTHLLFPHDSSL L RL QVYKILQ+VE YQKENKL   PI+LCGDWNGSKRGHV
Sbjct: 175  EILIVNTHLLFPHDSSLSLVRLNQVYKILQYVESYQKENKLSPTPIMLCGDWNGSKRGHV 234

Query: 751  YKFLRSQGFISSYDTAHEYTDTDAQKWVSHRNHRGNICGVDFIWLLNPSSYRKVLKVSWS 930
            YKFLRSQGF+SSYDTAH+YTD DA KWVSH NHRGNICGVDFIWLLNP+ YRK+LK SWS
Sbjct: 235  YKFLRSQGFVSSYDTAHQYTDADAHKWVSHLNHRGNICGVDFIWLLNPNRYRKLLKTSWS 294

Query: 931  ETVFGMFKCLLRRASLTADDAFAILKADSSGDFITYPGFCDALRQLNVTGEQYGLSMDET 1110
            E VFGMFK LLRRASLT +DAFA LKADS  D ITY GFC+AL+QLN+TG  YGLS +ET
Sbjct: 295  EAVFGMFKYLLRRASLTEEDAFAFLKADSDSDCITYSGFCEALQQLNLTGHCYGLSDEET 354

Query: 1111 KDLWEQADTDGNGIVDYKEFQLRIWNPTWSDQRFDINNEIRED-IVNATEQTVGYTVKNA 1287
            KDLW QAD DGNG++DYKEFQ RIWNPTWS+++ D   EI++D +    EQT+G++V+NA
Sbjct: 355  KDLWVQADIDGNGVLDYKEFQQRIWNPTWSEEKDD---EIQDDNLKGREEQTIGFSVENA 411

Query: 1288 VLFPNEVEKGMWPEDYSLSDHARLTVVFSPVKMLCSR 1398
            VLFP EVEKGMWPE+YSLSDHARLTVVFSP++M CS+
Sbjct: 412  VLFPPEVEKGMWPENYSLSDHARLTVVFSPIRMPCSQ 448


>ref|NP_001078754.1| calcium-binding endonuclease/exonuclease/phosphatase family protein
            [Arabidopsis thaliana] gi|27311783|gb|AAO00857.1|
            putative protein [Arabidopsis thaliana]
            gi|30387507|gb|AAP31919.1| At5g54130 [Arabidopsis
            thaliana] gi|51970738|dbj|BAD44061.1| putative protein
            [Arabidopsis thaliana] gi|332009070|gb|AED96453.1|
            calcium-binding endonuclease/exonuclease/phosphatase
            family protein [Arabidopsis thaliana]
          Length = 436

 Score =  640 bits (1651), Expect = 0.0
 Identities = 312/442 (70%), Positives = 360/442 (81%), Gaps = 6/442 (1%)
 Frame = +1

Query: 100  RGRLPRIQSYAIASD------PSSFSCTTFNILAPIYKRLDHQDQSCRESDNKDNWVNRN 261
            +GR+ +I SYAI+S           SCTTFNILAPIYKRL H+DQS RESDN+  W+ RN
Sbjct: 2    KGRISKIGSYAISSSIRDQHQQPCISCTTFNILAPIYKRLSHKDQSLRESDNRAYWLGRN 61

Query: 262  QRILDMLLYERSSIICLQEFWVGNNELVELYDKRLGDAGYITFKLARTNNRGDGLLTAVH 441
             RI+D LLYERSSIICLQEFWVGN ELV LY+KRLGDAGY+++KL RTNNRGDGLLTAVH
Sbjct: 62   HRIIDWLLYERSSIICLQEFWVGNEELVNLYEKRLGDAGYLSYKLGRTNNRGDGLLTAVH 121

Query: 442  KDYFRVINTEPVLLHGFGDRVAQLLHVEIATPFSQCRNNSVRQELLIVNTHLLFPHDSSL 621
            KDYFRV+N+  +L +  GDRVAQLLHVE+  P+SQ       QE+LIVNTHLLFPHDS+L
Sbjct: 122  KDYFRVVNSRDLLFNDCGDRVAQLLHVELVPPYSQY---DAHQEVLIVNTHLLFPHDSTL 178

Query: 622  CLERLRQVYKILQHVECYQKENKLHSLPILLCGDWNGSKRGHVYKFLRSQGFISSYDTAH 801
             + RL+QVYKILQ+VE YQKE  L  +PI+LCGDWNGSKRGHVYKFLRSQGF+SSYDTAH
Sbjct: 179  SIVRLQQVYKILQYVESYQKEVNLSPMPIILCGDWNGSKRGHVYKFLRSQGFVSSYDTAH 238

Query: 802  EYTDTDAQKWVSHRNHRGNICGVDFIWLLNPSSYRKVLKVSWSETVFGMFKCLLRRASLT 981
             YTD+DA KWVSHRNHRGNIC VDFIWLLNP+ YRK+LK SWSE VFGMF+ LLRRASLT
Sbjct: 239  RYTDSDAHKWVSHRNHRGNICAVDFIWLLNPNRYRKLLKTSWSEAVFGMFRYLLRRASLT 298

Query: 982  ADDAFAILKADSSGDFITYPGFCDALRQLNVTGEQYGLSMDETKDLWEQADTDGNGIVDY 1161
            A+DAFA LK D+ GD IT+ GFC+ LRQLN+TG   GL+  E KDLW QAD DGNG++DY
Sbjct: 299  AEDAFAFLKTDNDGDHITFMGFCETLRQLNLTGHCNGLTTKEIKDLWTQADIDGNGLLDY 358

Query: 1162 KEFQLRIWNPTWSDQRFDINNEIREDIVNATEQTVGYTVKNAVLFPNEVEKGMWPEDYSL 1341
            KEFQ RIWN TWS+QR   + E + +     EQTVG++VKNAVLFP EVEKGMWPE+YSL
Sbjct: 359  KEFQQRIWNQTWSEQRDAEDGEAKGN----QEQTVGFSVKNAVLFPPEVEKGMWPENYSL 414

Query: 1342 SDHARLTVVFSPVKMLCSRPTS 1407
            SDHARLTVVFSP++M CS+  S
Sbjct: 415  SDHARLTVVFSPIRMPCSQLVS 436