BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00009687
         (758 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002528573.1| Ran GTPase binding protein, putative [Ricinu...   335   5e-90
ref|XP_002284809.1| PREDICTED: probable E3 ubiquitin-protein lig...   325   6e-87
ref|NP_566512.1| regulator of chromosome condensation repeat-con...   323   2e-86
gb|AAK59406.1| unknown protein [Arabidopsis thaliana]                 323   2e-86
ref|XP_004141229.1| PREDICTED: probable E3 ubiquitin-protein lig...   322   7e-86

>ref|XP_002528573.1| Ran GTPase binding protein, putative [Ricinus communis]
           gi|223532017|gb|EEF33828.1| Ran GTPase binding protein,
           putative [Ricinus communis]
          Length = 497

 Score =  335 bits (860), Expect = 5e-90
 Identities = 152/202 (75%), Positives = 178/202 (88%)
 Frame = -3

Query: 756 VLSITKDGVVHSFGSATNFCLGHGAQHNELQPRAIQSFQRKGLQIVKVSAGDEHVVALDS 577
           VL++T  GVV+SFGS +NFCLGHG QHNE  PRAIQ+F+RK L +V+VSAGDEHVVALDS
Sbjct: 281 VLAVTDSGVVYSFGSGSNFCLGHGEQHNEFHPRAIQTFRRKNLHVVRVSAGDEHVVALDS 340

Query: 576 RGHVYTWGKGYCGALGHGDEIDKIIPEPLLCLRNKLIVQVCASKRKTFVLTENGSLYGFG 397
            G VYTWGKGYCGALGHGDEIDK +PEPL  L++ L VQVCA KRKTFVL + G ++GFG
Sbjct: 341 SGFVYTWGKGYCGALGHGDEIDKTLPEPLNSLKSHLAVQVCARKRKTFVLVDGGCVFGFG 400

Query: 396 WMGFGSLGFPDRGASDKILSPRVLDSLKGHRVSQVSTGLYHTVAVTTQGRIYGFGDNERA 217
           WMGFGSLGFPDRG SDK++ PR+LDSL+ HRVSQ+STGLYHTVAVT+QG+++GFGDNERA
Sbjct: 401 WMGFGSLGFPDRGVSDKVMRPRILDSLRAHRVSQISTGLYHTVAVTSQGQVFGFGDNERA 460

Query: 216 QLGHDSLRGCLKPTEIFFQQIG 151
           QLGHD+LRGCL+PTEIF Q+ G
Sbjct: 461 QLGHDTLRGCLEPTEIFLQEFG 482



 Score = 78.6 bits (192), Expect = 1e-12
 Identities = 56/185 (30%), Positives = 86/185 (46%), Gaps = 4/185 (2%)
 Frame = -3

Query: 747 ITKDGVVHSFGSATNFCLGHGAQHNEL-QPRAIQSFQRKGLQIVKVSAGDEHVVALDSRG 571
           I + G V + G  ++FC GH      + +PR +++   KG+   +V+AG    V L   G
Sbjct: 178 ILESGEVFTCGDNSSFCCGHQDTSRPIFRPRFVEAL--KGVPCKQVAAGLNFTVFLTRTG 235

Query: 570 HVYTWGKGYCGALGHGDEIDKIIPEPLLCLRN-KLIVQVCASKRKTFVLTENGSLYGFGW 394
           HVY+ G    G LGHGD +D+  P+ +        + Q+ A       +T++G +Y FG 
Sbjct: 236 HVYSCGTNTHGQLGHGDTLDRPTPKIIESFEGIGSVAQIAAGPSYVLAVTDSGVVYSFGS 295

Query: 393 MGFGSLGFPDRGASDKILSPRVLDSL--KGHRVSQVSTGLYHTVAVTTQGRIYGFGDNER 220
                LG    G       PR + +   K   V +VS G  H VA+ + G +Y +G    
Sbjct: 296 GSNFCLG---HGEQHNEFHPRAIQTFRRKNLHVVRVSAGDEHVVALDSSGFVYTWGKGYC 352

Query: 219 AQLGH 205
             LGH
Sbjct: 353 GALGH 357



 Score = 70.1 bits (170), Expect = 5e-10
 Identities = 45/140 (32%), Positives = 67/140 (47%), Gaps = 1/140 (0%)
 Frame = -3

Query: 621 VKVSAGDEHVVALDSRGHVYTWGKGYCGALGHGDEIDKIIPEPLLCLRNKL-IVQVCASK 445
           ++++ G  H + + S   VY+ G   CG LGHG E  + +    +   +   +VQV AS 
Sbjct: 114 MQITTGRYHTLLI-SNSSVYSCGSSLCGVLGHGPETTQCVSFSRITFPSLARVVQVSASH 172

Query: 444 RKTFVLTENGSLYGFGWMGFGSLGFPDRGASDKILSPRVLDSLKGHRVSQVSTGLYHTVA 265
                + E+G ++  G       G  D   S  I  PR +++LKG    QV+ GL  TV 
Sbjct: 173 NHAAYILESGEVFTCGDNSSFCCGHQD--TSRPIFRPRFVEALKGVPCKQVAAGLNFTVF 230

Query: 264 VTTQGRIYGFGDNERAQLGH 205
           +T  G +Y  G N   QLGH
Sbjct: 231 LTRTGHVYSCGTNTHGQLGH 250



 Score = 67.8 bits (164), Expect = 2e-09
 Identities = 56/189 (29%), Positives = 83/189 (43%), Gaps = 2/189 (1%)
 Frame = -3

Query: 729 VHSFGSATNFCLGHGAQHNELQPRAIQSFQRKGLQIVKVSAGDEHVVALDSRGHVYTWGK 550
           V+S GS+    LGHG +  +    +  +F     ++V+VSA   H   +   G V+T G 
Sbjct: 131 VYSCGSSLCGVLGHGPETTQCVSFSRITFPSLA-RVVQVSASHNHAAYILESGEVFTCGD 189

Query: 549 GYCGALGHGDEIDKII-PEPLLCLRNKLIVQVCASKRKTFVLTENGSLYGFGWMGFGSLG 373
                 GH D    I  P  +  L+     QV A    T  LT  G +Y  G    G LG
Sbjct: 190 NSSFCCGHQDTSRPIFRPRFVEALKGVPCKQVAAGLNFTVFLTRTGHVYSCGTNTHGQLG 249

Query: 372 FPDRGASDKILSPRVLDSLKG-HRVSQVSTGLYHTVAVTTQGRIYGFGDNERAQLGHDSL 196
             D    D+  +P++++S +G   V+Q++ G  + +AVT  G +Y FG      LGH   
Sbjct: 250 HGD--TLDRP-TPKIIESFEGIGSVAQIAAGPSYVLAVTDSGVVYSFGSGSNFCLGHGEQ 306

Query: 195 RGCLKPTEI 169
                P  I
Sbjct: 307 HNEFHPRAI 315


>ref|XP_002284809.1| PREDICTED: probable E3 ubiquitin-protein ligase HERC2 [Vitis
           vinifera] gi|302143037|emb|CBI20332.3| unnamed protein
           product [Vitis vinifera]
          Length = 492

 Score =  325 bits (833), Expect = 6e-87
 Identities = 149/202 (73%), Positives = 174/202 (86%)
 Frame = -3

Query: 753 LSITKDGVVHSFGSATNFCLGHGAQHNELQPRAIQSFQRKGLQIVKVSAGDEHVVALDSR 574
           +++T DG VHSFGS +NFCLGHG QHNEL+PRAIQSF+RK + +V++SAGDEHVVALDS 
Sbjct: 282 MAVTDDGTVHSFGSGSNFCLGHGEQHNELRPRAIQSFRRKTIHVVRISAGDEHVVALDSS 341

Query: 573 GHVYTWGKGYCGALGHGDEIDKIIPEPLLCLRNKLIVQVCASKRKTFVLTENGSLYGFGW 394
           G VYTWGKGYCGALGHGDEIDK  PE L  L++ L VQVCA KRKTFVL + GS+YGFGW
Sbjct: 342 GFVYTWGKGYCGALGHGDEIDKTTPEILSSLKSHLAVQVCARKRKTFVLVDTGSVYGFGW 401

Query: 393 MGFGSLGFPDRGASDKILSPRVLDSLKGHRVSQVSTGLYHTVAVTTQGRIYGFGDNERAQ 214
           MGFGSLGFPDRGASDK++ P +LDSL+GH +SQ+STGLYHTVAV  +G+I+GFGDNERAQ
Sbjct: 402 MGFGSLGFPDRGASDKVMRPWILDSLRGHYISQISTGLYHTVAVNNKGQIFGFGDNERAQ 461

Query: 213 LGHDSLRGCLKPTEIFFQQIGD 148
           LGHD+LRGC  PTEIF Q+  D
Sbjct: 462 LGHDTLRGCPVPTEIFVQEATD 483



 Score = 87.0 bits (214), Expect = 4e-15
 Identities = 59/185 (31%), Positives = 92/185 (49%), Gaps = 4/185 (2%)
 Frame = -3

Query: 747 ITKDGVVHSFGSATNFCLGHGAQHNEL-QPRAIQSFQRKGLQIVKVSAGDEHVVALDSRG 571
           + + G V + G  ++FC GHG     + +PR +++   KG+   +V+AG    V L  +G
Sbjct: 178 VMQSGEVFTCGDNSSFCCGHGDTVRPIFRPRLVEAL--KGVPCKQVAAGLSFTVFLTMKG 235

Query: 570 HVYTWGKGYCGALGHGDEIDKIIPEPLLCLRN-KLIVQVCASKRKTFVLTENGSLYGFGW 394
           HVYT G    G LGHGD +D+  P+ +  L     +VQ+ A       +T++G+++ FG 
Sbjct: 236 HVYTCGTNTHGQLGHGDTVDRRTPKIVELLEGVGPVVQIAAGPSYAMAVTDDGTVHSFGS 295

Query: 393 MGFGSLGFPDRGASDKILSPRVLDSL--KGHRVSQVSTGLYHTVAVTTQGRIYGFGDNER 220
                LG    G     L PR + S   K   V ++S G  H VA+ + G +Y +G    
Sbjct: 296 GSNFCLG---HGEQHNELRPRAIQSFRRKTIHVVRISAGDEHVVALDSSGFVYTWGKGYC 352

Query: 219 AQLGH 205
             LGH
Sbjct: 353 GALGH 357



 Score = 72.0 bits (175), Expect = 1e-10
 Identities = 58/195 (29%), Positives = 87/195 (44%), Gaps = 2/195 (1%)
 Frame = -3

Query: 747 ITKDGVVHSFGSATNFCLGHGAQHNELQPRAIQSFQRKGLQIVKVSAGDEHVVALDSRGH 568
           + KD  V+S GS+    LGHG +  +    +  +F      + +VSA   H   +   G 
Sbjct: 125 LIKDSSVYSCGSSLCGVLGHGPETTQCVAFSRINFPPLA-HVAQVSASHNHAAFVMQSGE 183

Query: 567 VYTWGKGYCGALGHGDEIDKII-PEPLLCLRNKLIVQVCASKRKTFVLTENGSLYGFGWM 391
           V+T G       GHGD +  I  P  +  L+     QV A    T  LT  G +Y  G  
Sbjct: 184 VFTCGDNSSFCCGHGDTVRPIFRPRLVEALKGVPCKQVAAGLSFTVFLTMKGHVYTCGTN 243

Query: 390 GFGSLGFPDRGASDKILSPRVLDSLKG-HRVSQVSTGLYHTVAVTTQGRIYGFGDNERAQ 214
             G LG  D    D+  +P++++ L+G   V Q++ G  + +AVT  G ++ FG      
Sbjct: 244 THGQLGHGD--TVDR-RTPKIVELLEGVGPVVQIAAGPSYAMAVTDDGTVHSFGSGSNFC 300

Query: 213 LGHDSLRGCLKPTEI 169
           LGH      L+P  I
Sbjct: 301 LGHGEQHNELRPRAI 315



 Score = 61.2 bits (147), Expect = 2e-07
 Identities = 41/140 (29%), Positives = 65/140 (46%), Gaps = 1/140 (0%)
 Frame = -3

Query: 621 VKVSAGDEHVVALDSRGHVYTWGKGYCGALGHGDEIDKIIPEPLLCLRNKL-IVQVCASK 445
           ++++ G  H + +     VY+ G   CG LGHG E  + +    +       + QV AS 
Sbjct: 114 MQITTGRYHTLLIKDSS-VYSCGSSLCGVLGHGPETTQCVAFSRINFPPLAHVAQVSASH 172

Query: 444 RKTFVLTENGSLYGFGWMGFGSLGFPDRGASDKILSPRVLDSLKGHRVSQVSTGLYHTVA 265
                + ++G ++  G       G  D      I  PR++++LKG    QV+ GL  TV 
Sbjct: 173 NHAAFVMQSGEVFTCGDNSSFCCGHGD--TVRPIFRPRLVEALKGVPCKQVAAGLSFTVF 230

Query: 264 VTTQGRIYGFGDNERAQLGH 205
           +T +G +Y  G N   QLGH
Sbjct: 231 LTMKGHVYTCGTNTHGQLGH 250


>ref|NP_566512.1| regulator of chromosome condensation repeat-containing protein
           [Arabidopsis thaliana] gi|42572455|ref|NP_974323.1|
           regulator of chromosome condensation repeat-containing
           protein [Arabidopsis thaliana] gi|7021728|gb|AAF35409.1|
           unknown protein [Arabidopsis thaliana]
           gi|15795108|dbj|BAB02372.1| unnamed protein product
           [Arabidopsis thaliana] gi|23297250|gb|AAN12924.1|
           unknown protein [Arabidopsis thaliana]
           gi|332642152|gb|AEE75673.1| regulator of chromosome
           condensation repeat-containing protein [Arabidopsis
           thaliana] gi|332642153|gb|AEE75674.1| regulator of
           chromosome condensation repeat-containing protein
           [Arabidopsis thaliana]
          Length = 488

 Score =  323 bits (829), Expect = 2e-86
 Identities = 152/197 (77%), Positives = 171/197 (86%)
 Frame = -3

Query: 756 VLSITKDGVVHSFGSATNFCLGHGAQHNELQPRAIQSFQRKGLQIVKVSAGDEHVVALDS 577
           VL++T+DG V+SFGS +NFCLGHG Q +ELQPR IQ+F+RKG+ I++VSAGDEH VALDS
Sbjct: 281 VLAVTQDGSVYSFGSGSNFCLGHGEQQDELQPRVIQAFKRKGIHILRVSAGDEHAVALDS 340

Query: 576 RGHVYTWGKGYCGALGHGDEIDKIIPEPLLCLRNKLIVQVCASKRKTFVLTENGSLYGFG 397
            G VYTWGKGYCGALGHGDE DKI P+ L+ L N L VQVCA KRKTFVL E G LYGFG
Sbjct: 341 NGRVYTWGKGYCGALGHGDENDKITPQVLVNLNNCLAVQVCARKRKTFVLVEGGLLYGFG 400

Query: 396 WMGFGSLGFPDRGASDKILSPRVLDSLKGHRVSQVSTGLYHTVAVTTQGRIYGFGDNERA 217
           WMGFGSLGFPDRG SDK+L PRVL+ LK HRVSQVSTGLYHT+ VT +GRI+GFGDNERA
Sbjct: 401 WMGFGSLGFPDRGVSDKVLRPRVLECLKPHRVSQVSTGLYHTIVVTQRGRIFGFGDNERA 460

Query: 216 QLGHDSLRGCLKPTEIF 166
           QLGHDSLRGCL+PTEIF
Sbjct: 461 QLGHDSLRGCLEPTEIF 477



 Score = 89.4 bits (220), Expect = 8e-16
 Identities = 59/185 (31%), Positives = 91/185 (49%), Gaps = 4/185 (2%)
 Frame = -3

Query: 747 ITKDGVVHSFGSATNFCLGH-GAQHNELQPRAIQSFQRKGLQIVKVSAGDEHVVALDSRG 571
           + + G V + G  ++ C GH        +P+ +++   KG    +V+AG    V L   G
Sbjct: 178 VLQSGQVLTCGDNSSHCCGHLDTSRPIFRPKLVEAL--KGTPCKQVAAGLHFTVFLSREG 235

Query: 570 HVYTWGKGYCGALGHGDEIDKIIPEPLLCLRN-KLIVQVCASKRKTFVLTENGSLYGFGW 394
           H YT G    G LGHGD +D+ +P+ +  L+    +VQ+ A       +T++GS+Y FG 
Sbjct: 236 HAYTCGSNTHGQLGHGDTLDRPVPKVVEFLKTIGPVVQIAAGPSYVLAVTQDGSVYSFGS 295

Query: 393 MGFGSLGFPDRGASDKILSPRVLDSL--KGHRVSQVSTGLYHTVAVTTQGRIYGFGDNER 220
                LG    G     L PRV+ +   KG  + +VS G  H VA+ + GR+Y +G    
Sbjct: 296 GSNFCLG---HGEQQDELQPRVIQAFKRKGIHILRVSAGDEHAVALDSNGRVYTWGKGYC 352

Query: 219 AQLGH 205
             LGH
Sbjct: 353 GALGH 357



 Score = 58.5 bits (140), Expect = 1e-06
 Identities = 43/159 (27%), Positives = 76/159 (47%), Gaps = 2/159 (1%)
 Frame = -3

Query: 621 VKVSAGDEHVVALDSRGHVYTWGKGYCGALGHGDEIDKIIP-EPLLCLRNKLIVQVCASK 445
           ++++ G  H + +++   VY+ G    G L HG E  + +   P+       + QV A++
Sbjct: 114 MQIATGKYHTLLINN-SKVYSCGVSLSGVLAHGSETTQCVAFTPIEFPFPAQVAQVSATQ 172

Query: 444 RKTFVLTENGSLYGFGWMGFGSLGFPDRGASDKILSPRVLDSLKGHRVSQVSTGLYHTVA 265
             +  + ++G +   G       G  D   S  I  P+++++LKG    QV+ GL+ TV 
Sbjct: 173 NHSAFVLQSGQVLTCGDNSSHCCGHLD--TSRPIFRPKLVEALKGTPCKQVAAGLHFTVF 230

Query: 264 VTTQGRIYGFGDNERAQLGH-DSLRGCLKPTEIFFQQIG 151
           ++ +G  Y  G N   QLGH D+L   +     F + IG
Sbjct: 231 LSREGHAYTCGSNTHGQLGHGDTLDRPVPKVVEFLKTIG 269



 Score = 57.4 bits (137), Expect = 3e-06
 Identities = 55/189 (29%), Positives = 80/189 (42%), Gaps = 2/189 (1%)
 Frame = -3

Query: 729 VHSFGSATNFCLGHGAQHNELQPRAIQSFQRKGLQIVKVSAGDEHVVALDSRGHVYTWGK 550
           V+S G + +  L HG++  +        F     Q+ +VSA   H   +   G V T G 
Sbjct: 131 VYSCGVSLSGVLAHGSETTQCVAFTPIEFPFPA-QVAQVSATQNHSAFVLQSGQVLTCGD 189

Query: 549 GYCGALGHGDEIDKII-PEPLLCLRNKLIVQVCASKRKTFVLTENGSLYGFGWMGFGSLG 373
                 GH D    I  P+ +  L+     QV A    T  L+  G  Y  G    G LG
Sbjct: 190 NSSHCCGHLDTSRPIFRPKLVEALKGTPCKQVAAGLHFTVFLSREGHAYTCGSNTHGQLG 249

Query: 372 FPDRGASDKILSPRVLDSLKG-HRVSQVSTGLYHTVAVTTQGRIYGFGDNERAQLGHDSL 196
             D    D+ + P+V++ LK    V Q++ G  + +AVT  G +Y FG      LGH   
Sbjct: 250 HGD--TLDRPV-PKVVEFLKTIGPVVQIAAGPSYVLAVTQDGSVYSFGSGSNFCLGHGEQ 306

Query: 195 RGCLKPTEI 169
           +  L+P  I
Sbjct: 307 QDELQPRVI 315


>gb|AAK59406.1| unknown protein [Arabidopsis thaliana]
          Length = 488

 Score =  323 bits (828), Expect = 2e-86
 Identities = 152/197 (77%), Positives = 171/197 (86%)
 Frame = -3

Query: 756 VLSITKDGVVHSFGSATNFCLGHGAQHNELQPRAIQSFQRKGLQIVKVSAGDEHVVALDS 577
           VL++T+DG V+SFGS +NFCLGHG Q +ELQPR IQ+F+RKG+ I++VSAGDEH VALDS
Sbjct: 281 VLALTQDGSVYSFGSGSNFCLGHGEQQDELQPRVIQAFKRKGIHILRVSAGDEHAVALDS 340

Query: 576 RGHVYTWGKGYCGALGHGDEIDKIIPEPLLCLRNKLIVQVCASKRKTFVLTENGSLYGFG 397
            G VYTWGKGYCGALGHGDE DKI P+ L+ L N L VQVCA KRKTFVL E G LYGFG
Sbjct: 341 NGRVYTWGKGYCGALGHGDENDKITPQVLVNLNNCLAVQVCARKRKTFVLVEGGLLYGFG 400

Query: 396 WMGFGSLGFPDRGASDKILSPRVLDSLKGHRVSQVSTGLYHTVAVTTQGRIYGFGDNERA 217
           WMGFGSLGFPDRG SDK+L PRVL+ LK HRVSQVSTGLYHT+ VT +GRI+GFGDNERA
Sbjct: 401 WMGFGSLGFPDRGVSDKVLRPRVLECLKPHRVSQVSTGLYHTIVVTQRGRIFGFGDNERA 460

Query: 216 QLGHDSLRGCLKPTEIF 166
           QLGHDSLRGCL+PTEIF
Sbjct: 461 QLGHDSLRGCLEPTEIF 477



 Score = 90.5 bits (223), Expect = 3e-16
 Identities = 60/185 (32%), Positives = 91/185 (49%), Gaps = 4/185 (2%)
 Frame = -3

Query: 747 ITKDGVVHSFGSATNFCLGH-GAQHNELQPRAIQSFQRKGLQIVKVSAGDEHVVALDSRG 571
           + + G V + G  ++ C GH        +P+ +++   KG    +V+AG    V L   G
Sbjct: 178 VLQSGQVLTCGDNSSHCCGHLDTSRPIFRPKLVEAL--KGTPCKQVAAGLHFTVFLSREG 235

Query: 570 HVYTWGKGYCGALGHGDEIDKIIPEPLLCLRN-KLIVQVCASKRKTFVLTENGSLYGFGW 394
           H YT G    G LGHGD +D+ +P+ +  L+    +VQ+ A       LT++GS+Y FG 
Sbjct: 236 HAYTCGSNTHGQLGHGDTLDRPVPKVVEFLKTIGPVVQIAAGPSYVLALTQDGSVYSFGS 295

Query: 393 MGFGSLGFPDRGASDKILSPRVLDSL--KGHRVSQVSTGLYHTVAVTTQGRIYGFGDNER 220
                LG    G     L PRV+ +   KG  + +VS G  H VA+ + GR+Y +G    
Sbjct: 296 GSNFCLG---HGEQQDELQPRVIQAFKRKGIHILRVSAGDEHAVALDSNGRVYTWGKGYC 352

Query: 219 AQLGH 205
             LGH
Sbjct: 353 GALGH 357



 Score = 58.5 bits (140), Expect = 1e-06
 Identities = 43/159 (27%), Positives = 76/159 (47%), Gaps = 2/159 (1%)
 Frame = -3

Query: 621 VKVSAGDEHVVALDSRGHVYTWGKGYCGALGHGDEIDKIIP-EPLLCLRNKLIVQVCASK 445
           ++++ G  H + +++   VY+ G    G L HG E  + +   P+       + QV A++
Sbjct: 114 MQIATGKYHTLLINN-SKVYSCGVSLSGVLAHGSETTQCVAFTPIEFPFPAQVAQVSATQ 172

Query: 444 RKTFVLTENGSLYGFGWMGFGSLGFPDRGASDKILSPRVLDSLKGHRVSQVSTGLYHTVA 265
             +  + ++G +   G       G  D   S  I  P+++++LKG    QV+ GL+ TV 
Sbjct: 173 NHSAFVLQSGQVLTCGDNSSHCCGHLD--TSRPIFRPKLVEALKGTPCKQVAAGLHFTVF 230

Query: 264 VTTQGRIYGFGDNERAQLGH-DSLRGCLKPTEIFFQQIG 151
           ++ +G  Y  G N   QLGH D+L   +     F + IG
Sbjct: 231 LSREGHAYTCGSNTHGQLGHGDTLDRPVPKVVEFLKTIG 269



 Score = 56.2 bits (134), Expect = 7e-06
 Identities = 54/189 (28%), Positives = 80/189 (42%), Gaps = 2/189 (1%)
 Frame = -3

Query: 729 VHSFGSATNFCLGHGAQHNELQPRAIQSFQRKGLQIVKVSAGDEHVVALDSRGHVYTWGK 550
           V+S G + +  L HG++  +        F     Q+ +VSA   H   +   G V T G 
Sbjct: 131 VYSCGVSLSGVLAHGSETTQCVAFTPIEFPFPA-QVAQVSATQNHSAFVLQSGQVLTCGD 189

Query: 549 GYCGALGHGDEIDKII-PEPLLCLRNKLIVQVCASKRKTFVLTENGSLYGFGWMGFGSLG 373
                 GH D    I  P+ +  L+     QV A    T  L+  G  Y  G    G LG
Sbjct: 190 NSSHCCGHLDTSRPIFRPKLVEALKGTPCKQVAAGLHFTVFLSREGHAYTCGSNTHGQLG 249

Query: 372 FPDRGASDKILSPRVLDSLKG-HRVSQVSTGLYHTVAVTTQGRIYGFGDNERAQLGHDSL 196
             D    D+ + P+V++ LK    V Q++ G  + +A+T  G +Y FG      LGH   
Sbjct: 250 HGD--TLDRPV-PKVVEFLKTIGPVVQIAAGPSYVLALTQDGSVYSFGSGSNFCLGHGEQ 306

Query: 195 RGCLKPTEI 169
           +  L+P  I
Sbjct: 307 QDELQPRVI 315


>ref|XP_004141229.1| PREDICTED: probable E3 ubiquitin-protein ligase HERC2-like [Cucumis
           sativus]
          Length = 491

 Score =  322 bits (824), Expect = 7e-86
 Identities = 148/203 (72%), Positives = 170/203 (83%)
 Frame = -3

Query: 756 VLSITKDGVVHSFGSATNFCLGHGAQHNELQPRAIQSFQRKGLQIVKVSAGDEHVVALDS 577
           VL++  DG V+SFGS +NFCLGHG QHNE QPR +Q+F+ K + +V VSAGDEH VALDS
Sbjct: 281 VLAVVNDGRVYSFGSGSNFCLGHGEQHNEFQPRVLQTFRLKNIHVVSVSAGDEHAVALDS 340

Query: 576 RGHVYTWGKGYCGALGHGDEIDKIIPEPLLCLRNKLIVQVCASKRKTFVLTENGSLYGFG 397
            G VYTWGKGYCGALGHGDEIDK IP+PL  L+N L VQVCA KRKTFVL + G +YGFG
Sbjct: 341 NGLVYTWGKGYCGALGHGDEIDKTIPQPLNSLKNHLAVQVCARKRKTFVLVDTGIVYGFG 400

Query: 396 WMGFGSLGFPDRGASDKILSPRVLDSLKGHRVSQVSTGLYHTVAVTTQGRIYGFGDNERA 217
           WMGFGSLGFPDRG SDK+L PR LDSL+ HRVSQ+STGLYHTV VT +G+I+GFGDNERA
Sbjct: 401 WMGFGSLGFPDRGVSDKVLRPRSLDSLRVHRVSQISTGLYHTVVVTEKGKIFGFGDNERA 460

Query: 216 QLGHDSLRGCLKPTEIFFQQIGD 148
           QLGH+SLRGCL+PTEIF   + D
Sbjct: 461 QLGHESLRGCLEPTEIFIHDLDD 483



 Score = 69.7 bits (169), Expect = 6e-10
 Identities = 54/169 (31%), Positives = 79/169 (46%), Gaps = 8/169 (4%)
 Frame = -3

Query: 636 KGLQIVKVSAGDEHVVA------LDSRGHVYTWGKGYCGALGHGDEIDKIIPEPLLCLRN 475
           K   IV+ S G+  +        L S G VY+ G   CG LGHG E  + +    +    
Sbjct: 102 KSSNIVETSEGNMQIATGRYHTLLISNGSVYSCGSSLCGVLGHGSETTQCVTFTRINFPP 161

Query: 474 KLIV-QVCASKRKTFVLTENGSLYGFGWMGFGSLGFPDRGASDKILSPRVLDSLKGHRVS 298
            + V QV AS      +T++G ++  G       G  D   S +I  PR++++L G    
Sbjct: 162 SIYVEQVSASHNHAAFVTQSGQVFTCGDNSSFCCGHRD--TSCRIFRPRLVEALNGIPCK 219

Query: 297 QVSTGLYHTVAVTTQGRIYGFGDNERAQLGH-DSLRGCLKPTEIFFQQI 154
           QVS G   +V +T QG +Y  G N   QLGH ++L G   PT    Q++
Sbjct: 220 QVSVGHNFSVFLTRQGHVYTCGTNTYGQLGHGNTLEG---PTPKIVQRL 265



 Score = 62.4 bits (150), Expect = 1e-07
 Identities = 51/192 (26%), Positives = 82/192 (42%), Gaps = 2/192 (1%)
 Frame = -3

Query: 747 ITKDGVVHSFGSATNFCLGHGAQHNELQPRAIQSFQRKGLQIVKVSAGDEHVVALDSRGH 568
           +  +G V+S GS+    LGHG++  +       +F    + + +VSA   H   +   G 
Sbjct: 125 LISNGSVYSCGSSLCGVLGHGSETTQCVTFTRINFP-PSIYVEQVSASHNHAAFVTQSGQ 183

Query: 567 VYTWGKGYCGALGHGDEIDKII-PEPLLCLRNKLIVQVCASKRKTFVLTENGSLYGFGWM 391
           V+T G       GH D   +I  P  +  L      QV      +  LT  G +Y  G  
Sbjct: 184 VFTCGDNSSFCCGHRDTSCRIFRPRLVEALNGIPCKQVSVGHNFSVFLTRQGHVYTCGTN 243

Query: 390 GFGSLGFPDRGASDKILSPRVLDSL-KGHRVSQVSTGLYHTVAVTTQGRIYGFGDNERAQ 214
            +G LG    G + +  +P+++  L +   V Q++ G  + +AV   GR+Y FG      
Sbjct: 244 TYGQLG---HGNTLEGPTPKIVQRLVEIGSVIQIAAGPSYVLAVVNDGRVYSFGSGSNFC 300

Query: 213 LGHDSLRGCLKP 178
           LGH       +P
Sbjct: 301 LGHGEQHNEFQP 312