BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00009650
         (1641 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002280704.1| PREDICTED: chloroplastic group IIA intron sp...   758   0.0  
ref|XP_002516757.1| conserved hypothetical protein [Ricinus comm...   705   0.0  
ref|XP_003551841.1| PREDICTED: chloroplastic group IIA intron sp...   697   0.0  
ref|XP_002329296.1| predicted protein [Populus trichocarpa] gi|2...   677   0.0  
ref|NP_197122.2| ortholog of maize chloroplast splicing factor C...   624   e-176

>ref|XP_002280704.1| PREDICTED: chloroplastic group IIA intron splicing facilitator CRS1,
            chloroplastic-like [Vitis vinifera]
          Length = 1184

 Score =  758 bits (1958), Expect = 0.0
 Identities = 380/597 (63%), Positives = 463/597 (77%), Gaps = 50/597 (8%)
 Frame = +1

Query: 1    GERMPWEEDERAVFRRMKKEKVVTAAELSLDSVLLERLRSEAAKMRKWVKVKKAGVTGDV 180
            G +MPW + E+ VFRR KKEKVVTAAEL+LD +LLERLR EA KMRKWVKVKKAGVT  V
Sbjct: 173  GGKMPWLKTEKVVFRRTKKEKVVTAAELTLDPMLLERLRGEAVKMRKWVKVKKAGVTESV 232

Query: 181  VHQMRIIWRNNELVMLKFDLPLCRNMDRAREIVELKTGGLVVWTKKDAHVIYRGSNYQYP 360
            V Q+ ++W+++EL M+KFD+PLCRNMDRAREI+E+KT GLV+W+KKD  V+YRGSNYQ  
Sbjct: 233  VDQIHMVWKSDELAMVKFDMPLCRNMDRAREILEIKTRGLVIWSKKDTLVVYRGSNYQST 292

Query: 361  KPFIRK---------------------NDDL----------------GRDDGKEEIIP-- 423
                +K                      DDL                GR DG+E+  P  
Sbjct: 293  SKHFQKMRPGLVAGADASNSKLNQSNFEDDLTISEIKFHESTTGEKMGRKDGEEDSSPTG 352

Query: 424  -----------IDGSLYEREADRLLDGLGPRFIDWWRPKPLPVDADLLPEEVPGFRPPFR 570
                       ++GSLYEREADRLLDGLGPRFIDWWRPKPLPVDADLLPE +PGFRPPFR
Sbjct: 353  IFMEEMVDSQPVNGSLYEREADRLLDGLGPRFIDWWRPKPLPVDADLLPEVLPGFRPPFR 412

Query: 571  LCPPKTRSKLSDDELTYLRRLSHPLPTHFVLGRNSKLQGLAAAILKLWEKCHIAKIALKL 750
            L PP+TRSKL+DDELTYLR+L++ LPTHFVLGRN KLQGLAAAILKLWEK  I KIA+K 
Sbjct: 413  LSPPQTRSKLTDDELTYLRKLAYALPTHFVLGRNRKLQGLAAAILKLWEKSLIVKIAIKW 472

Query: 751  GVPNTNNEEMANELKCLTGGVLILRNKFIIIIYRGKDFLPCRVADLVAEREMELKKCQLQ 930
            G+PNT NE+MANELKCLTGGVL+LRNKF II+YRGKDFLPCRVA+L+ EREME K CQ++
Sbjct: 473  GIPNTKNEQMANELKCLTGGVLLLRNKFFIILYRGKDFLPCRVANLIVEREMEFKGCQIR 532

Query: 931  EEAARAEAIGTISLTIEPSFGGSSIGTLSEFQDIRSDSEDYEGADQKVNIQFEAEKRKRE 1110
            EE AR +AI T  +T +P    S+ GTLSEFQ+I ++    +  + ++ ++ EAEK + E
Sbjct: 533  EEDARLKAIETSFVTDKPLANTSTTGTLSEFQNIETEFRGLKDGNTEIEVELEAEKERLE 592

Query: 1111 KQLRQHEHKLSILNKKIWRSTRNIEKLNSAWAPAEMDADQEIVTPEEKECLRKIGLKMDS 1290
            K+L++ E  L IL +KI RS + + KLNSAW PA+ DAD+E++T EE+EC RKIG KMDS
Sbjct: 593  KELKKQERNLFILKRKIERSAKVLAKLNSAWRPADHDADKEMITEEERECFRKIGQKMDS 652

Query: 1291 TLVLGRRGVFDGVIEGLHQHWKHKEIIKVITKQRVFSQVVNTAKLLQRQSGGILISVEEL 1470
            +L+LGRRGVFDGVIEGLHQHWKH+EI+KVIT QR FSQV+ TAKLL+ +SGG+L+S+++L
Sbjct: 653  SLLLGRRGVFDGVIEGLHQHWKHREIVKVITMQRSFSQVLYTAKLLESESGGVLVSIDKL 712

Query: 1471 KEGHAILLYRGKNYRRPVKLHSENLLSKRKALERSLEMQRVGSLKYFAYQRQNAISD 1641
            KEGHAI++YRGKNYRRP+KL  +NLL+KR+AL RSLEMQR+GSLK+FAYQRQ AISD
Sbjct: 713  KEGHAIIIYRGKNYRRPIKLVPKNLLTKREALNRSLEMQRIGSLKFFAYQRQQAISD 769


>ref|XP_002516757.1| conserved hypothetical protein [Ricinus communis]
            gi|223544130|gb|EEF45655.1| conserved hypothetical
            protein [Ricinus communis]
          Length = 742

 Score =  705 bits (1819), Expect = 0.0
 Identities = 362/558 (64%), Positives = 437/558 (78%), Gaps = 15/558 (2%)
 Frame = +1

Query: 13   PWEEDERAVFRRMKKEKVVTAAELSLDSVLLERLRSEAAKMRKWVKVKKAGVTGDVVHQM 192
            PWE +E+ V+ R+KKEK VT AEL L+  LLE LR+EA+KMRKWVKV KAGVT  VV Q+
Sbjct: 173  PWEREEKFVYWRIKKEKAVTKAELILEKELLEILRTEASKMRKWVKVMKAGVTQSVVDQI 232

Query: 193  RIIWRNNELVMLKFDLPLCRNMDRAREIVELKTGGLVVWTKKDAHVIYRGSNYQYPKP-- 366
            R  WRNNEL M+KFDLPLCRNMDRAREIVELKTGGLVVWT+KD+ VIYRG NY   K   
Sbjct: 233  RYAWRNNELAMVKFDLPLCRNMDRAREIVELKTGGLVVWTRKDSLVIYRGCNYHLTKSSH 292

Query: 367  FIRKNDDLGRDDGKEEIIP-------------IDGSLYEREADRLLDGLGPRFIDWWRPK 507
                ++ +G  DG+EE IP             I+GSL+ERE DRLLDGLGPRF+DWW  K
Sbjct: 293  VSTMDEKIGSKDGEEEYIPTSIFIGDDANTPTINGSLFERETDRLLDGLGPRFVDWWMRK 352

Query: 508  PLPVDADLLPEEVPGFRPPFRLCPPKTRSKLSDDELTYLRRLSHPLPTHFVLGRNSKLQG 687
            PLPVDADLLPE V GF PP R      R+KL DDELTYLR+L++ LPTHFVLGRN +LQG
Sbjct: 353  PLPVDADLLPEVVAGFMPPSRF--HYARAKLKDDELTYLRKLAYALPTHFVLGRNRRLQG 410

Query: 688  LAAAILKLWEKCHIAKIALKLGVPNTNNEEMANELKCLTGGVLILRNKFIIIIYRGKDFL 867
            LAAAILKLWE+  IAKIA+K G+PNT+NE+MANELK LTGGVL+LRNKF II++RGKDFL
Sbjct: 411  LAAAILKLWERSLIAKIAVKWGIPNTDNEQMANELKHLTGGVLLLRNKFFIILFRGKDFL 470

Query: 868  PCRVADLVAEREMELKKCQLQEEAARAEAIGTISLTIEPSFGGSSIGTLSEFQDIRSDSE 1047
            PC+VADLV +RE ELK CQL EE AR +AI T     E     + IGTL+EFQDI+   +
Sbjct: 471  PCQVADLVVKRENELKICQLNEEGARLKAIETSFTDDELVVKATKIGTLNEFQDIQVRFK 530

Query: 1048 DYEGADQKVNIQFEAEKRKREKQLRQHEHKLSILNKKIWRSTRNIEKLNSAWAPAEMDAD 1227
            +     +   +Q EAEK K E++LR  EHKL IL  KI +S R + KLNSAWAPA+ DAD
Sbjct: 531  ELAKGYRDSKLQLEAEKEKLERELRIQEHKLLILKSKIEKSARELSKLNSAWAPADQDAD 590

Query: 1228 QEIVTPEEKECLRKIGLKMDSTLVLGRRGVFDGVIEGLHQHWKHKEIIKVITKQRVFSQV 1407
             E++T EE+ECLRKIGLKM S+L+LGRRGVFDGVIEGLHQHWKH+E++KVI+ QR+F+QV
Sbjct: 591  LEMMTEEERECLRKIGLKMRSSLLLGRRGVFDGVIEGLHQHWKHREVVKVISLQRMFAQV 650

Query: 1408 VNTAKLLQRQSGGILISVEELKEGHAILLYRGKNYRRPVKLHSENLLSKRKALERSLEMQ 1587
            + TAK L+ ++GGIL+S+++LKEGHAI++YRGKNYRRP +L   NLL+KRKAL RSLEMQ
Sbjct: 651  IRTAKFLEAETGGILVSIDKLKEGHAIIIYRGKNYRRPQRL-LNNLLTKRKALCRSLEMQ 709

Query: 1588 RVGSLKYFAYQRQNAISD 1641
            R+GSL++FAYQRQ++I +
Sbjct: 710  RIGSLRFFAYQRQHSIRE 727


>ref|XP_003551841.1| PREDICTED: chloroplastic group IIA intron splicing facilitator CRS1,
            chloroplastic-like [Glycine max]
          Length = 712

 Score =  697 bits (1800), Expect = 0.0
 Identities = 349/551 (63%), Positives = 436/551 (79%), Gaps = 6/551 (1%)
 Frame = +1

Query: 7    RMPWEEDERAVFRRMKKEKVVTAAELSLDSVLLERLRSEAAKMRKWVKVKKAGVTGDVVH 186
            RMPWE+DE+  F ++K+EK VTAAEL+LD  LL RLR+EAA+MR W+KVKKAGVT DVV 
Sbjct: 144  RMPWEKDEKFGFVKVKREKAVTAAELTLDKALLRRLRNEAARMRTWIKVKKAGVTQDVVD 203

Query: 187  QMRIIWRNNELVMLKFDLPLCRNMDRAREIVELKTGGLVVWTKKDAHVIYRGSNYQYPKP 366
            Q++  WR NEL M+KFD+PLCRNMDRAREIVE KTGGLVV +KKD  V+YRG N+ +   
Sbjct: 204  QIKRTWRRNELAMIKFDIPLCRNMDRAREIVETKTGGLVVLSKKDFLVVYRGCNH-HSSE 262

Query: 367  FIRKNDD------LGRDDGKEEIIPIDGSLYEREADRLLDGLGPRFIDWWRPKPLPVDAD 528
             +  N D       G  D   +++  +GSLYERE +RLLDGLGPRFIDWW  KPLPVDAD
Sbjct: 263  MLNWNADHKDSISTGIQDVNCQLV--NGSLYERETERLLDGLGPRFIDWWMHKPLPVDAD 320

Query: 529  LLPEEVPGFRPPFRLCPPKTRSKLSDDELTYLRRLSHPLPTHFVLGRNSKLQGLAAAILK 708
            LLPEEVPGF+PPFRLCPP + +KL+D ELTY R+L+  LPTHFVLGRN  L+GLA+AILK
Sbjct: 321  LLPEEVPGFQPPFRLCPPHSSAKLTDYELTYFRKLAQSLPTHFVLGRNKGLKGLASAILK 380

Query: 709  LWEKCHIAKIALKLGVPNTNNEEMANELKCLTGGVLILRNKFIIIIYRGKDFLPCRVADL 888
            LWEK  IAKIA+K G+PNT+NE MANELKCLTGGVL+LRNKF I++YRG DFLP  VA L
Sbjct: 381  LWEKSLIAKIAIKYGIPNTDNEMMANELKCLTGGVLLLRNKFYILLYRGNDFLPRSVASL 440

Query: 889  VAEREMELKKCQLQEEAARAEAIGTISLTIEPSFGGSSIGTLSEFQDIRSDSEDYEGADQ 1068
            V +RE+ELK  QL EE AR +AI   S   E     S+ GTL+EF+ I++  ED +  + 
Sbjct: 441  VEKRELELKSRQLHEEVARMKAIQAFSPIDEVPLDTSTSGTLTEFRKIQTKLEDTKSVNV 500

Query: 1069 KVNIQFEAEKRKREKQLRQHEHKLSILNKKIWRSTRNIEKLNSAWAPAEMDADQEIVTPE 1248
              NIQ EAE  + EK+L++ + +  ILNKKI RS R + KLN+AW P+E D D EI+T E
Sbjct: 501  DSNIQLEAEICRLEKELKEEQRRAFILNKKIKRSERELSKLNAAWTPSEQDTDLEIMTDE 560

Query: 1249 EKECLRKIGLKMDSTLVLGRRGVFDGVIEGLHQHWKHKEIIKVITKQRVFSQVVNTAKLL 1428
            E+EC RKIGLKM S+L+LGRRG+FDGV+EGLHQHWKH+E++KVIT Q++FSQV+NTAK+L
Sbjct: 561  ERECFRKIGLKMQSSLLLGRRGIFDGVLEGLHQHWKHREVVKVITMQKLFSQVINTAKVL 620

Query: 1429 QRQSGGILISVEELKEGHAILLYRGKNYRRPVKLHSENLLSKRKALERSLEMQRVGSLKY 1608
            + +SGGIL+SV++LKEGHAI++YRGKNY+RP    ++NLL+KR+AL RSLEMQR+GS+K+
Sbjct: 621  ETESGGILVSVDKLKEGHAIIIYRGKNYKRPSIKLAKNLLTKREALRRSLEMQRIGSMKF 680

Query: 1609 FAYQRQNAISD 1641
            FA+QR+ AIS+
Sbjct: 681  FAHQREQAISE 691


>ref|XP_002329296.1| predicted protein [Populus trichocarpa] gi|222870750|gb|EEF07881.1|
            predicted protein [Populus trichocarpa]
          Length = 687

 Score =  677 bits (1746), Expect = 0.0
 Identities = 348/558 (62%), Positives = 429/558 (76%), Gaps = 12/558 (2%)
 Frame = +1

Query: 4    ERMPWEEDERAVFRRMKKEKVVTAAELSLDSVLLERLRSEAAKMRKWVKVKKAGVTGDVV 183
            +++PW  +ER    RMKKEKVV+ AELSLD  LLERLR EAAKMR WVKVKKAGVT  VV
Sbjct: 115  KKLPWVREERVGNWRMKKEKVVSKAELSLDKELLERLRGEAAKMRTWVKVKKAGVTQSVV 174

Query: 184  HQMRIIWRNNELVMLKFDLPLCRNMDRAREIVELKTGGLVVWTKKDAHVIYRGSNYQYPK 363
             ++R+ WR +EL M+KF +PLCRNM+RAR+IVE  TGGLVVWT+KD HV+YRG NYQ+  
Sbjct: 175  DEIRLTWRTSELAMIKFYMPLCRNMNRARDIVE--TGGLVVWTRKDIHVVYRGCNYQW-- 230

Query: 364  PFIRKNDDLGRDDGKEEIIPIDGSLYEREADRLLDGLGPRFIDWWRPKPLPVDADLLPEE 543
               +KN +    +      PI+GSL+ERE DRLLDGLGPRF+DWW  KPLPVDADLLPE 
Sbjct: 231  ---KKNFNTATIEENLNTQPINGSLFERETDRLLDGLGPRFVDWWMRKPLPVDADLLPEV 287

Query: 544  VPGFRPPFRLCPPKTRSKLSDDELTYLRRLSHPLPTHFVLGRNSKLQGLAAAILKLWEKC 723
            V GFR P RLCPP+ RSKL DDELTYLR+L+  LPTHFVLGRN +LQGLAAAILKLWEK 
Sbjct: 288  VKGFRSPSRLCPPRMRSKLKDDELTYLRKLAQSLPTHFVLGRNRRLQGLAAAILKLWEKT 347

Query: 724  HIAKIALKLGVPNTNNEEMANELKC------------LTGGVLILRNKFIIIIYRGKDFL 867
             IAKIA+K GVPNTNNE+MA+ELK             LTGGVL+LRNKF II+YRGKDFL
Sbjct: 348  IIAKIAVKWGVPNTNNEQMADELKAKIFLMLMLYTQSLTGGVLLLRNKFFIILYRGKDFL 407

Query: 868  PCRVADLVAEREMELKKCQLQEEAARAEAIGTISLTIEPSFGGSSIGTLSEFQDIRSDSE 1047
            P +VA+++ +RE+ L+KCQ  EE AR +AI T  +   P+   S  GTL EFQ+ +   +
Sbjct: 408  PGQVANVIVDREIALRKCQTNEEGARMKAIETSYMPGGPT-NTSRCGTLYEFQEFQIKFQ 466

Query: 1048 DYEGADQKVNIQFEAEKRKREKQLRQHEHKLSILNKKIWRSTRNIEKLNSAWAPAEMDAD 1227
                 D +  IQ EA K K E++LR  E++L IL  KI +  +++ KLNSAW P+  DAD
Sbjct: 467  KTAKGDSE--IQLEAYKEKLERELRNQEYRLRILKSKIEKPAKDLSKLNSAWVPSPRDAD 524

Query: 1228 QEIVTPEEKECLRKIGLKMDSTLVLGRRGVFDGVIEGLHQHWKHKEIIKVITKQRVFSQV 1407
            Q I+T EE+EC RKIGLK+  +LVLGRRGVF+GV+EGLHQHWKH+E++KVIT QRVFSQV
Sbjct: 525  QGIMTEEERECFRKIGLKLRGSLVLGRRGVFEGVMEGLHQHWKHREVVKVITMQRVFSQV 584

Query: 1408 VNTAKLLQRQSGGILISVEELKEGHAILLYRGKNYRRPVKLHSENLLSKRKALERSLEMQ 1587
            ++TA LL+ +S GIL+SV++LKEGHAI++YRGKNY+RP++L  +NLL+KR+AL+RSL +Q
Sbjct: 585  IHTATLLEAESDGILVSVDKLKEGHAIIIYRGKNYKRPLRLLKKNLLTKREALKRSLLIQ 644

Query: 1588 RVGSLKYFAYQRQNAISD 1641
            RVGSLKYFA QR+  ISD
Sbjct: 645  RVGSLKYFANQRERVISD 662


>ref|NP_197122.2| ortholog of maize chloroplast splicing factor CRS1 [Arabidopsis
            thaliana] gi|374095377|sp|Q9LF10.2|CRS1_ARATH RecName:
            Full=Chloroplastic group IIA intron splicing facilitator
            CRS1, chloroplastic; AltName: Full=Chloroplastic RNA
            splicing factor 1; AltName: Full=Protein CHLOROPLAST RNA
            SPLICING 1; Flags: Precursor gi|332004875|gb|AED92258.1|
            ortholog of maize chloroplast splicing factor CRS1
            [Arabidopsis thaliana]
          Length = 720

 Score =  624 bits (1610), Expect = e-176
 Identities = 329/552 (59%), Positives = 415/552 (75%), Gaps = 5/552 (0%)
 Frame = +1

Query: 1    GERMPWE-EDERAVFRRMKKEKVVTAAELSLDSVLLERLRSEAAKMRKWVKVKKAGVTGD 177
            G +MPWE E+ER + RRMKKE V T AEL LD  LL RLR EA+KMRKWV V+KAGVT  
Sbjct: 177  GGKMPWEREEERFILRRMKKESVPTTAELILDEGLLNRLRREASKMRKWVNVRKAGVTEL 236

Query: 178  VVHQMRIIWRNNELVMLKFDLPLCRNMDRAREIVELKTGGLVVWTKKDAHVIYRGS-NYQ 354
            VV++++ +W+ NEL M++FD+PLCRNM+RA+EI+E+KTGGLVV +KK+  V+YRG  +Y 
Sbjct: 237  VVNKIKSMWKLNELAMVRFDVPLCRNMERAQEIIEMKTGGLVVLSKKEFLVVYRGGPSYS 296

Query: 355  YPKPFIRKNDDLGRDDGKEEIIPIDGSLYEREADRLLDGLGPRFIDWWRPKPLPVDADLL 534
                           +G++EI     SLYEREADRLLDGLGPR++DWW  +P PVDADLL
Sbjct: 297  --------------SEGQDEI---SSSLYEREADRLLDGLGPRYMDWWMRRPFPVDADLL 339

Query: 535  PEEVPGFRPPFRLCPPKTRSKLSDDELTYLRRLSHPLPTHFVLGRNSKLQGLAAAILKLW 714
            PE V G+  P R CPP TR+KL+D+ELTYLR ++ PLP HFVLGRN  LQGLA+AI+KLW
Sbjct: 340  PEVVNGYMTPSRRCPPNTRAKLTDEELTYLRNIAQPLPFHFVLGRNYGLQGLASAIVKLW 399

Query: 715  EKCHIAKIALKLGVPNTNNEEMANELKCLTGGVLILRNKFIIIIYRGKDFLPCRVADLVA 894
            EKC IAKIA+K G  NTNNEEMA+EL+ LTGGVLILRNK++I++YRGKDFL   VADLV 
Sbjct: 400  EKCIIAKIAIKWGALNTNNEEMADELRYLTGGVLILRNKYLIVLYRGKDFLSDEVADLVE 459

Query: 895  EREMELKKCQLQEEAARAEAIGTISLTI--EPSFGGSSIGTLSEFQDIRSDSEDYEGADQ 1068
            +RE  L + Q  EE  R   I  + +    +     +  GTL EFQ+++       G   
Sbjct: 460  DRERLLSRYQHFEETKRESDIELLEVVTNGKQLKETNKSGTLLEFQELQRKF----GEMD 515

Query: 1069 KVNIQFEAEKRKREKQLRQHEHKLSILNKKIWRSTRNIEKLNSAWAPAEMDADQEIVTPE 1248
              N++ EAEK + EK+L+  EHKLSIL  KI +S   + KLNS W P+E D D EI+T E
Sbjct: 516  PRNLETEAEKARLEKELKSQEHKLSILKSKIEKSNMELFKLNSLWKPSEGDDDIEILTNE 575

Query: 1249 EKECLRKIGLKMDSTLVLGRRGVFDGVIEGLHQHWKHKEIIKVITKQRVFSQVVNTAKLL 1428
            E+ECLR+IGLKM+S+LVLGRRGVF GV+EGLHQHWKH+E+ KVIT Q++FS+VV TAK L
Sbjct: 576  ERECLRRIGLKMNSSLVLGRRGVFFGVMEGLHQHWKHREVAKVITMQKLFSRVVYTAKAL 635

Query: 1429 QRQSGGILISVEELKEGHAILLYRGKNYRRP-VKLHSENLLSKRKALERSLEMQRVGSLK 1605
            + +S G+LIS+E+LKEGHAIL+YRGKNY+RP  KL ++NLL+KRKAL+RS+ MQR+GSLK
Sbjct: 636  ETESNGVLISIEKLKEGHAILIYRGKNYKRPSSKLMAQNLLTKRKALQRSVVMQRLGSLK 695

Query: 1606 YFAYQRQNAISD 1641
            +FAYQR+ AI D
Sbjct: 696  FFAYQRERAIED 707