BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00009614
         (1830 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002272810.2| PREDICTED: protein MID1-COMPLEMENTING ACTIVI...   610   e-172
dbj|BAK32933.1| mid1-complementing activity 2 [Nicotiana tabacum]     605   e-170
ref|XP_002327738.1| predicted protein [Populus trichocarpa] gi|2...   595   e-167
dbj|BAK32932.1| mid1-complementing activity 1 [Nicotiana tabacum]     589   e-166
ref|XP_003537651.1| PREDICTED: protein MID1-COMPLEMENTING ACTIVI...   573   e-161

>ref|XP_002272810.2| PREDICTED: protein MID1-COMPLEMENTING ACTIVITY 1-like [Vitis
            vinifera]
          Length = 420

 Score =  610 bits (1572), Expect = e-172
 Identities = 296/397 (74%), Positives = 335/397 (84%), Gaps = 1/397 (0%)
 Frame = +2

Query: 638  ASWEHFGEIANVAQLTGLDAVRLIGLIVKAAATARMHKKNCRQFATHLKLVGNLLEQLKI 817
            A+WEHFGEIANVAQLTG+DAVRLIG+IV AA TARMHKKNCRQFA HLKL+GNLLEQLKI
Sbjct: 2    ATWEHFGEIANVAQLTGVDAVRLIGMIVSAANTARMHKKNCRQFAQHLKLIGNLLEQLKI 61

Query: 818  SELKKYPETREPLEYLEDALRRSYILVNSCQDRSYLYLLAMGWNIVYQFRRAQNEIDQYL 997
            SELK+YPE REPLE LED+LRR YILVNSCQDRSYLYLLAMGWNIVYQFR+AQNEID+YL
Sbjct: 62   SELKRYPEMREPLEQLEDSLRRGYILVNSCQDRSYLYLLAMGWNIVYQFRKAQNEIDRYL 121

Query: 998  KIIPLITLVDNARVRERLEVINMDRCEYTLDEEDRKIQDVIMKPEPSKNDTVVLKKTLSC 1177
            KIIPLITLVDNARVRERLE I  D+ EYTLD+ED+++QDVI+KPEPS NDTVVLKKTLSC
Sbjct: 122  KIIPLITLVDNARVRERLEDIEKDQREYTLDDEDKRVQDVILKPEPSTNDTVVLKKTLSC 181

Query: 1178 SYPNMPFKEAIKKENEKLQLELQHSQAHLDVSQCEVIQHLIDVTEAVAVSSPPEKNLPVK 1357
            SYPN+PF  A+KKENEKLQLELQ SQA+ DV QCEVIQHLI VTEAVA +S PEKNLPV+
Sbjct: 182  SYPNLPFNAALKKENEKLQLELQRSQANYDVDQCEVIQHLIGVTEAVAANSVPEKNLPVR 241

Query: 1358 KSTKLEPSYSNTSSIKD-QYDDVYTKKKETFXXXXXXXXXXXXXXXXXXXXXQRNEDWNS 1534
             S K E  YS+ +S K+   D+ Y K+ +T                       R+E+W+S
Sbjct: 242  SSKKAESDYSDANSDKEYSVDESYPKRSDTRTASRNISSVSSGHDLLSTRGSHRHEEWHS 301

Query: 1535 DLLGCCAEPALCMKTFFFPCGTFSRIASVANDRHISSAEACNEMMAYSLILSCCCYTCCV 1714
            DLLGCC+EP+LC+KTFF+PCGTFS+IASVA +RH+SSAEACNE+MAYS+ILSCCCYTCC+
Sbjct: 302  DLLGCCSEPSLCIKTFFYPCGTFSKIASVATNRHMSSAEACNELMAYSMILSCCCYTCCI 361

Query: 1715 RRKLRKTLKIAGGFCDDFLSHVMCCCCALVQEWREVE 1825
            RRKLR  L I GG  DDFLSH+MCCCCALVQEWREVE
Sbjct: 362  RRKLRNMLNITGGLFDDFLSHLMCCCCALVQEWREVE 398


>dbj|BAK32933.1| mid1-complementing activity 2 [Nicotiana tabacum]
          Length = 419

 Score =  605 bits (1560), Expect = e-170
 Identities = 296/396 (74%), Positives = 326/396 (82%)
 Frame = +2

Query: 638  ASWEHFGEIANVAQLTGLDAVRLIGLIVKAAATARMHKKNCRQFATHLKLVGNLLEQLKI 817
            ASWEHFGEIANVAQLTG+DAVRLIG+IVKAA TARMHKKNCRQFA HLKL+G+LLEQLKI
Sbjct: 2    ASWEHFGEIANVAQLTGIDAVRLIGMIVKAATTARMHKKNCRQFAQHLKLIGSLLEQLKI 61

Query: 818  SELKKYPETREPLEYLEDALRRSYILVNSCQDRSYLYLLAMGWNIVYQFRRAQNEIDQYL 997
            +ELK+YPETREPLE LEDALRRSYILV SCQDRSYLYLLAMGWNIVYQFRRAQNEIDQYL
Sbjct: 62   TELKRYPETREPLEQLEDALRRSYILVKSCQDRSYLYLLAMGWNIVYQFRRAQNEIDQYL 121

Query: 998  KIIPLITLVDNARVRERLEVINMDRCEYTLDEEDRKIQDVIMKPEPSKNDTVVLKKTLSC 1177
            KIIPLITLVDNARVRERLE I  D+ EYTL+ ED K+Q+VIMKPEPSK+DT++L K LSC
Sbjct: 122  KIIPLITLVDNARVRERLEFIERDQHEYTLEAEDMKVQEVIMKPEPSKDDTIILTKNLSC 181

Query: 1178 SYPNMPFKEAIKKENEKLQLELQHSQAHLDVSQCEVIQHLIDVTEAVAVSSPPEKNLPVK 1357
            SYP +P  EAIKKENEKLQLELQ SQA+LDV QCE IQHL+DVTE VA +S   K+ P+K
Sbjct: 182  SYPRVPINEAIKKENEKLQLELQRSQANLDVGQCEFIQHLLDVTEVVATNSLSLKSSPIK 241

Query: 1358 KSTKLEPSYSNTSSIKDQYDDVYTKKKETFXXXXXXXXXXXXXXXXXXXXXQRNEDWNSD 1537
               KL+ SYSN  S  D Y + Y K  E                        R E+W+SD
Sbjct: 242  PPKKLDESYSNVDSYTDHYVESYAKNDEKQATSRNASSVSSKHELLSSKGSHRYEEWHSD 301

Query: 1538 LLGCCAEPALCMKTFFFPCGTFSRIASVANDRHISSAEACNEMMAYSLILSCCCYTCCVR 1717
            LLGCC+EP LC+KT FFPCGTFS++ASVA DRHISSAEACNE+MAYSLILSCCCYTCC+R
Sbjct: 302  LLGCCSEPLLCIKTVFFPCGTFSKVASVAADRHISSAEACNELMAYSLILSCCCYTCCIR 361

Query: 1718 RKLRKTLKIAGGFCDDFLSHVMCCCCALVQEWREVE 1825
            RKLRK L I GGF DDFLSH+MCCCCALVQEWREVE
Sbjct: 362  RKLRKKLNIMGGFVDDFLSHLMCCCCALVQEWREVE 397


>ref|XP_002327738.1| predicted protein [Populus trichocarpa] gi|222836823|gb|EEE75216.1|
            predicted protein [Populus trichocarpa]
          Length = 419

 Score =  595 bits (1534), Expect = e-167
 Identities = 283/396 (71%), Positives = 330/396 (83%)
 Frame = +2

Query: 638  ASWEHFGEIANVAQLTGLDAVRLIGLIVKAAATARMHKKNCRQFATHLKLVGNLLEQLKI 817
            A+WEH GE+ANV QLTG+DAVRLI +I KAA TARMHKKNCRQFA HLKL+GNLLEQLKI
Sbjct: 2    ATWEHLGEVANVVQLTGIDAVRLIAMIGKAATTARMHKKNCRQFAQHLKLIGNLLEQLKI 61

Query: 818  SELKKYPETREPLEYLEDALRRSYILVNSCQDRSYLYLLAMGWNIVYQFRRAQNEIDQYL 997
            SELK+YPETREPLE LEDALRRSY+LVNSCQDRSYLYLLAMGWNIVYQFR+AQNEID+YL
Sbjct: 62   SELKRYPETREPLEQLEDALRRSYLLVNSCQDRSYLYLLAMGWNIVYQFRKAQNEIDRYL 121

Query: 998  KIIPLITLVDNARVRERLEVINMDRCEYTLDEEDRKIQDVIMKPEPSKNDTVVLKKTLSC 1177
            +++PLITLVDN+RVRERLE I  D+ EYTLD+EDR++QDVI+KP+ S   T +LKKTLSC
Sbjct: 122  RLVPLITLVDNSRVRERLEYIERDQREYTLDDEDRRVQDVILKPDCSGEHTTMLKKTLSC 181

Query: 1178 SYPNMPFKEAIKKENEKLQLELQHSQAHLDVSQCEVIQHLIDVTEAVAVSSPPEKNLPVK 1357
            SYPNM F EA++KENEKLQLELQ SQAHLDV+QCEVIQHLI+VTE  A SS PEK+   K
Sbjct: 182  SYPNMCFNEALRKENEKLQLELQRSQAHLDVNQCEVIQHLIEVTEVAAASSLPEKSSSTK 241

Query: 1358 KSTKLEPSYSNTSSIKDQYDDVYTKKKETFXXXXXXXXXXXXXXXXXXXXXQRNEDWNSD 1537
             S KLEP+YS+ S  K  +DD Y+ K ++                       + E+W++D
Sbjct: 242  SSKKLEPAYSDASENKHSFDDSYSTKSDSHKTSRNTSSVSSRDDLLSSRGSHQQEEWHAD 301

Query: 1538 LLGCCAEPALCMKTFFFPCGTFSRIASVANDRHISSAEACNEMMAYSLILSCCCYTCCVR 1717
            LLGCC+EP LC+KT F+PCGTF++IA+VA +RHISSAEACNE+MAYS++LSCCCYTCCVR
Sbjct: 302  LLGCCSEPYLCIKTLFYPCGTFAKIATVAKNRHISSAEACNELMAYSMMLSCCCYTCCVR 361

Query: 1718 RKLRKTLKIAGGFCDDFLSHVMCCCCALVQEWREVE 1825
            R+LRKTL I GGF DDFLSH+MCCCCALVQEWREVE
Sbjct: 362  RELRKTLNITGGFIDDFLSHLMCCCCALVQEWREVE 397


>dbj|BAK32932.1| mid1-complementing activity 1 [Nicotiana tabacum]
          Length = 419

 Score =  589 bits (1518), Expect = e-166
 Identities = 288/396 (72%), Positives = 327/396 (82%)
 Frame = +2

Query: 638  ASWEHFGEIANVAQLTGLDAVRLIGLIVKAAATARMHKKNCRQFATHLKLVGNLLEQLKI 817
            A+WEHFGE+AN AQL GLDAVRLIG+IVKAA+TARMHKKNCRQFA HLKL+GNLLEQLKI
Sbjct: 2    ATWEHFGEVANFAQLAGLDAVRLIGMIVKAASTARMHKKNCRQFAQHLKLIGNLLEQLKI 61

Query: 818  SELKKYPETREPLEYLEDALRRSYILVNSCQDRSYLYLLAMGWNIVYQFRRAQNEIDQYL 997
            +ELKKYPETREPLEYLEDALRRSY+LV+SC DRSYLYLLAMGWNIVYQFR+AQNEIDQYL
Sbjct: 62   TELKKYPETREPLEYLEDALRRSYMLVHSCHDRSYLYLLAMGWNIVYQFRKAQNEIDQYL 121

Query: 998  KIIPLITLVDNARVRERLEVINMDRCEYTLDEEDRKIQDVIMKPEPSKNDTVVLKKTLSC 1177
            KIIPLITLVDNARVRER E+I  D+CEYTL+ ED K+Q+VI+K EPSK+DTVVLKKTLS 
Sbjct: 122  KIIPLITLVDNARVRERFEIIEKDQCEYTLEAEDMKVQEVILKREPSKHDTVVLKKTLSR 181

Query: 1178 SYPNMPFKEAIKKENEKLQLELQHSQAHLDVSQCEVIQHLIDVTEAVAVSSPPEKNLPVK 1357
            SYP+MP  EAI+KENEKLQLELQ SQA+LDVS CE IQHL++VTE VA  S  EK+ P K
Sbjct: 182  SYPSMPINEAIQKENEKLQLELQRSQANLDVSHCEFIQHLLEVTEVVASESLSEKSSPTK 241

Query: 1358 KSTKLEPSYSNTSSIKDQYDDVYTKKKETFXXXXXXXXXXXXXXXXXXXXXQRNEDWNSD 1537
             + KLE S+S+ +S K+  D  YTK  E                        R ++W+SD
Sbjct: 242  PTKKLEHSHSDVNSDKEHNDRSYTKSDEKQSTSRITSSVSSQRELLSSKGSDRYDEWHSD 301

Query: 1538 LLGCCAEPALCMKTFFFPCGTFSRIASVANDRHISSAEACNEMMAYSLILSCCCYTCCVR 1717
            LLGCC+EP LC+KT FFPCGTFS++AS A DRHISSA+ACNE+MAYSLILSCCCYTCC+R
Sbjct: 302  LLGCCSEPLLCIKTCFFPCGTFSKVASAAADRHISSADACNELMAYSLILSCCCYTCCIR 361

Query: 1718 RKLRKTLKIAGGFCDDFLSHVMCCCCALVQEWREVE 1825
            +KLRK L I GG  DDFLSH+MCCCCALVQE REVE
Sbjct: 362  KKLRKKLNITGGIIDDFLSHLMCCCCALVQELREVE 397


>ref|XP_003537651.1| PREDICTED: protein MID1-COMPLEMENTING ACTIVITY 1-like [Glycine max]
          Length = 416

 Score =  573 bits (1476), Expect = e-161
 Identities = 285/396 (71%), Positives = 323/396 (81%)
 Frame = +2

Query: 638  ASWEHFGEIANVAQLTGLDAVRLIGLIVKAAATARMHKKNCRQFATHLKLVGNLLEQLKI 817
            ASW+  GE+ANVAQLTG+DAVRLIG+IV+AA+TARMHKKNCRQFA HLKL+GNLLEQLKI
Sbjct: 2    ASWDQMGELANVAQLTGVDAVRLIGMIVRAASTARMHKKNCRQFAQHLKLIGNLLEQLKI 61

Query: 818  SELKKYPETREPLEYLEDALRRSYILVNSCQDRSYLYLLAMGWNIVYQFRRAQNEIDQYL 997
            SELKKYPETREPLE LEDALRRSYILVNSCQDRSYLYLLAMGWNIVYQFR+AQNEID+YL
Sbjct: 62   SELKKYPETREPLEQLEDALRRSYILVNSCQDRSYLYLLAMGWNIVYQFRKAQNEIDRYL 121

Query: 998  KIIPLITLVDNARVRERLEVINMDRCEYTLDEEDRKIQDVIMKPEPSKNDTVVLKKTLSC 1177
            +++PLITLVDN+RVRERLEVI MD+ EYTLD+ED+K Q VI KPEP K+DT VLKKTLSC
Sbjct: 122  RLVPLITLVDNSRVRERLEVIEMDQREYTLDDEDQKAQTVIFKPEPDKDDTAVLKKTLSC 181

Query: 1178 SYPNMPFKEAIKKENEKLQLELQHSQAHLDVSQCEVIQHLIDVTEAVAVSSPPEKNLPVK 1357
            SYPN  F EA+KKENEKL+LELQ SQA+LD++QCEVIQ L+DVTE VA  S P K  P K
Sbjct: 182  SYPNCSFTEALKKENEKLKLELQRSQANLDMNQCEVIQRLLDVTE-VAAYSVPAKCSPEK 240

Query: 1358 KSTKLEPSYSNTSSIKDQYDDVYTKKKETFXXXXXXXXXXXXXXXXXXXXXQRNEDWNSD 1537
               K E +YS+ +S +D   D   K                           + EDW++D
Sbjct: 241  SHKKEEYNYSDVNSDQDHSSD--EKYHAKIDKHSPSRYSVAQKDLASTGGSYQQEDWHTD 298

Query: 1538 LLGCCAEPALCMKTFFFPCGTFSRIASVANDRHISSAEACNEMMAYSLILSCCCYTCCVR 1717
            LL CC+EP+LCMKTFF+PCGTFS+IASVA +R ISS EACN++MAYSLILSCCCYTCCVR
Sbjct: 299  LLACCSEPSLCMKTFFYPCGTFSKIASVARNRPISSGEACNDLMAYSLILSCCCYTCCVR 358

Query: 1718 RKLRKTLKIAGGFCDDFLSHVMCCCCALVQEWREVE 1825
            RKLRK L I GGF DDFLSH+MCCCCALVQEWREVE
Sbjct: 359  RKLRKMLNITGGFIDDFLSHLMCCCCALVQEWREVE 394