BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00009460
         (1915 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFK79027.1| cytochrome P450 CYP90D18 [Bupleurum chinense]         1021   0.0  
emb|CBI35940.3| unnamed protein product [Vitis vinifera]              728   0.0  
ref|XP_002275659.2| PREDICTED: 3-epi-6-deoxocathasterone 23-mono...   725   0.0  
ref|XP_002527414.1| cytochrome P450, putative [Ricinus communis]...   723   0.0  
ref|XP_002327539.1| cytochrome P450 probable 6-deoxoteasterone t...   703   0.0  

>gb|AFK79027.1| cytochrome P450 CYP90D18 [Bupleurum chinense]
          Length = 512

 Score = 1021 bits (2639), Expect = 0.0
 Identities = 507/512 (99%), Positives = 508/512 (99%)
 Frame = -3

Query: 1727 MEDSWIMVVMAILFLSFILILYMYTWRSTSNSTKCRKSETSLPLGTLGWPLVGETMEFIS 1548
            MEDSWIMVVMAILFLSFILILYMYTWRSTSNSTKCRKSE SLPLGTLGWPLVGETMEFIS
Sbjct: 1    MEDSWIMVVMAILFLSFILILYMYTWRSTSNSTKCRKSEASLPLGTLGWPLVGETMEFIS 60

Query: 1547 CAYTDQPETFMDKRRRMYGKVFKSHIFGSPTIVSTDEEVSRNILQGDSNTFVPSYPKSLT 1368
            CAYTDQPETFM+KRRRMYGKVFKSHIFGSPTIVSTDEEVSRNILQGDSNTFVPSYPKSLT
Sbjct: 61   CAYTDQPETFMEKRRRMYGKVFKSHIFGSPTIVSTDEEVSRNILQGDSNTFVPSYPKSLT 120

Query: 1367 ILMGESSILLINGYLQRRIHGLIGSFLKSPNLKAQITRDMQKFVQQSMGSWDEDRLIYIQ 1188
            ILMGESSILLINGYLQRRIHGLIGSFLKSPNLKAQITRDMQKFVQQSMGSWDEDRLIYIQ
Sbjct: 121  ILMGESSILLINGYLQRRIHGLIGSFLKSPNLKAQITRDMQKFVQQSMGSWDEDRLIYIQ 180

Query: 1187 DETKKIAFQVLVKALISLDPGDEMEFLRKQFQEFIAGLMSLPINIPGTRLYRSLQAKKKM 1008
            DETKKI FQVLVKALISLDPGDEMEFLRKQFQEFIAGLMSLPINIPGTRLYRSLQAKKKM
Sbjct: 181  DETKKIGFQVLVKALISLDPGDEMEFLRKQFQEFIAGLMSLPINIPGTRLYRSLQAKKKM 240

Query: 1007 VKLMGKIIEAKKNGGSSKVPKDVAEVLLNENMKDDIISDNMIDLMIPGEDSVPVLITLAI 828
            VKLMGKIIEAKKNGGSSKVPKDVAEVLLNENMKDDIISDNMIDLMIPGEDSVPVLITLAI
Sbjct: 241  VKLMGKIIEAKKNGGSSKVPKDVAEVLLNENMKDDIISDNMIDLMIPGEDSVPVLITLAI 300

Query: 827  KYLSDCPAALQQLLEENMKLKALKDQMGEEMIWSDYLSLPFTQTVITETLRLGNIINGVM 648
            KYLSDCPAALQQLLEENMKLKALKDQ GEEMIWSDYLSLPFTQTVITETLRLGNIINGVM
Sbjct: 301  KYLSDCPAALQQLLEENMKLKALKDQTGEEMIWSDYLSLPFTQTVITETLRLGNIINGVM 360

Query: 647  RKAMKDVQLKGGYVIPKGWCVFTYFRSVHLDENLYDSPHQFNPWRWQDRDTSGCSFTPFG 468
            RKAMKDVQLKGGYVIPKGWCVFTYFRSVHLDENLYDSPHQFNPWRW DRDTSGCSFTPFG
Sbjct: 361  RKAMKDVQLKGGYVIPKGWCVFTYFRSVHLDENLYDSPHQFNPWRWPDRDTSGCSFTPFG 420

Query: 467  GGQRLCPGLDLARLEVSIFLHHFVTQFKWVAEDDSIVNFPTVRMKNKMPIWVKRRSSHDA 288
            GGQRLCPGLDLARLEVSIFLHHFVTQFKWVAEDDSIVNFPTVRMKNKMPIWVKRRSSHDA
Sbjct: 421  GGQRLCPGLDLARLEVSIFLHHFVTQFKWVAEDDSIVNFPTVRMKNKMPIWVKRRSSHDA 480

Query: 287  FGLKSKLNFEGSSKNDTERDDQSARQYEKEIV 192
            FGLKSKLNFEGSSKNDTERDDQSARQYEKEIV
Sbjct: 481  FGLKSKLNFEGSSKNDTERDDQSARQYEKEIV 512


>emb|CBI35940.3| unnamed protein product [Vitis vinifera]
          Length = 475

 Score =  728 bits (1880), Expect = 0.0
 Identities = 363/483 (75%), Positives = 420/483 (86%), Gaps = 5/483 (1%)
 Frame = -3

Query: 1727 MEDSWIMVVMAILFLSFILILYMYTWRSTSNSTKCRKS-ETSLPLGTLGWPLVGETMEFI 1551
            M + WI+ + AI FLS I++LY        N ++ R S  +SLPLGTLGWPL+GET+EFI
Sbjct: 1    MHNLWIVFLTAI-FLSTIILLYR-------NRSRIRSSPSSSLPLGTLGWPLIGETLEFI 52

Query: 1550 SCAYTDQPETFMDKRRRMYGKVFKSHIFGSPTIVSTDEEVSRNILQGDSNTFVPSYPKSL 1371
            SCAY+D+PE+FM++RRRMYGKVFKSHIFGSPTIVS D EVSR +LQ DS  FVPSYPKSL
Sbjct: 53   SCAYSDRPESFMERRRRMYGKVFKSHIFGSPTIVSIDAEVSRFVLQSDSKAFVPSYPKSL 112

Query: 1370 TILMGESSILLINGYLQRRIHGLIGSFLKSPNLKAQITRDMQKFVQQSMGSWDEDRLIYI 1191
            T LMG+SSILLING LQRR+HGLIG+F KSP+LKAQIT++M+ ++Q+SMGSW +D  I+I
Sbjct: 113  TELMGQSSILLINGSLQRRVHGLIGAFFKSPHLKAQITQEMESYIQKSMGSWRDDHPIFI 172

Query: 1190 QDETKKIAFQVLVKALISLDPGDEMEFLRKQFQEFIAGLMSLPINIPGTRLYRSLQAKKK 1011
            QDE K IAFQVLVKALISL+PG+EMEFLRKQFQEFI+GLMSLP+NIPGTRLYRSLQAKKK
Sbjct: 173  QDEAKNIAFQVLVKALISLNPGEEMEFLRKQFQEFISGLMSLPVNIPGTRLYRSLQAKKK 232

Query: 1010 MVKLMGKIIEAKKN-GGSSKVPKDVAEVLLNEN---MKDDIISDNMIDLMIPGEDSVPVL 843
            MV+L+GKII+ ++N    SKVPKDV +VLLN++   + D +ISDNMIDLMIPGEDSVPVL
Sbjct: 233  MVQLVGKIIQERRNINQPSKVPKDVLDVLLNDSSQLLTDTLISDNMIDLMIPGEDSVPVL 292

Query: 842  ITLAIKYLSDCPAALQQLLEENMKLKALKDQMGEEMIWSDYLSLPFTQTVITETLRLGNI 663
            +TLAIKYLSDCPAALQQL EENM+LK LK + GE M WSDYLSLPFTQTVITETLRLGN+
Sbjct: 293  VTLAIKYLSDCPAALQQLTEENMRLKRLKAERGETMTWSDYLSLPFTQTVITETLRLGNV 352

Query: 662  INGVMRKAMKDVQLKGGYVIPKGWCVFTYFRSVHLDENLYDSPHQFNPWRWQDRDTSGCS 483
            I GVMRKAMKDV++K G+ IPKGWCVF YFRSVHLDE+ YD P+QFNPWRWQD++ S CS
Sbjct: 353  IIGVMRKAMKDVEIK-GHRIPKGWCVFAYFRSVHLDESQYDWPYQFNPWRWQDKNISSCS 411

Query: 482  FTPFGGGQRLCPGLDLARLEVSIFLHHFVTQFKWVAEDDSIVNFPTVRMKNKMPIWVKRR 303
            FTPFGGGQRLCPGLDLARLE SIFLHHFVTQF+WVAEDDSIVNFPTVRMK +MP+WVKRR
Sbjct: 412  FTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWVAEDDSIVNFPTVRMKRRMPVWVKRR 471

Query: 302  SSH 294
              +
Sbjct: 472  RDY 474


>ref|XP_002275659.2| PREDICTED: 3-epi-6-deoxocathasterone 23-monooxygenase-like [Vitis
            vinifera]
          Length = 478

 Score =  725 bits (1872), Expect = 0.0
 Identities = 360/476 (75%), Positives = 415/476 (87%), Gaps = 5/476 (1%)
 Frame = -3

Query: 1706 VVMAILFLSFILILYMYTWRSTSNSTKCRKS-ETSLPLGTLGWPLVGETMEFISCAYTDQ 1530
            VV   +FLS I++LY        N ++ R S  +SLPLGTLGWPL+GET+EFISCAY+D+
Sbjct: 10   VVPTAIFLSTIILLYR-------NRSRIRSSPSSSLPLGTLGWPLIGETLEFISCAYSDR 62

Query: 1529 PETFMDKRRRMYGKVFKSHIFGSPTIVSTDEEVSRNILQGDSNTFVPSYPKSLTILMGES 1350
            PE+FM++RRRMYGKVFKSHIFGSPTIVS D EVSR +LQ DS  FVPSYPKSLT LMG+S
Sbjct: 63   PESFMERRRRMYGKVFKSHIFGSPTIVSIDAEVSRFVLQSDSKAFVPSYPKSLTELMGQS 122

Query: 1349 SILLINGYLQRRIHGLIGSFLKSPNLKAQITRDMQKFVQQSMGSWDEDRLIYIQDETKKI 1170
            SILLING LQRR+HGLIG+F KSP+LKAQIT++M+ ++Q+SMGSW +D  I+IQDE K I
Sbjct: 123  SILLINGSLQRRVHGLIGAFFKSPHLKAQITQEMESYIQKSMGSWRDDHPIFIQDEAKNI 182

Query: 1169 AFQVLVKALISLDPGDEMEFLRKQFQEFIAGLMSLPINIPGTRLYRSLQAKKKMVKLMGK 990
            AFQVLVKALISL+PG+EMEFLRKQFQEFI+GLMSLP+NIPGTRLYRSLQAKKKMV+L+GK
Sbjct: 183  AFQVLVKALISLNPGEEMEFLRKQFQEFISGLMSLPVNIPGTRLYRSLQAKKKMVQLVGK 242

Query: 989  IIEAKKN-GGSSKVPKDVAEVLLNEN---MKDDIISDNMIDLMIPGEDSVPVLITLAIKY 822
            II+ ++N    SKVPKDV +VLLN++   + D +ISDNMIDLMIPGEDSVPVL+TLAIKY
Sbjct: 243  IIQERRNINQPSKVPKDVLDVLLNDSSQLLTDTLISDNMIDLMIPGEDSVPVLVTLAIKY 302

Query: 821  LSDCPAALQQLLEENMKLKALKDQMGEEMIWSDYLSLPFTQTVITETLRLGNIINGVMRK 642
            LSDCPAALQQL EENM+LK LK + GE M WSDYLSLPFTQTVITETLRLGN+I GVMRK
Sbjct: 303  LSDCPAALQQLTEENMRLKRLKAERGETMTWSDYLSLPFTQTVITETLRLGNVIIGVMRK 362

Query: 641  AMKDVQLKGGYVIPKGWCVFTYFRSVHLDENLYDSPHQFNPWRWQDRDTSGCSFTPFGGG 462
            AMKDV++K G+ IPKGWCVF YFRSVHLDE+ YD P+QFNPWRWQD++ S CSFTPFGGG
Sbjct: 363  AMKDVEIK-GHRIPKGWCVFAYFRSVHLDESQYDWPYQFNPWRWQDKNISSCSFTPFGGG 421

Query: 461  QRLCPGLDLARLEVSIFLHHFVTQFKWVAEDDSIVNFPTVRMKNKMPIWVKRRSSH 294
            QRLCPGLDLARLE SIFLHHFVTQF+WVAEDDSIVNFPTVRMK +MP+WVKRR  +
Sbjct: 422  QRLCPGLDLARLEASIFLHHFVTQFRWVAEDDSIVNFPTVRMKRRMPVWVKRRRDY 477


>ref|XP_002527414.1| cytochrome P450, putative [Ricinus communis]
            gi|223533224|gb|EEF34980.1| cytochrome P450, putative
            [Ricinus communis]
          Length = 482

 Score =  723 bits (1867), Expect = 0.0
 Identities = 359/484 (74%), Positives = 418/484 (86%), Gaps = 3/484 (0%)
 Frame = -3

Query: 1721 DSWIMVVMAILFLSFILILYMYTWRSTSNSTKCRKSETSLPLGTLGWPLVGETMEFISCA 1542
            DS +++V+ IL LS ++ILY    R  S   +  K++T LPLG LGWP +GET+EF+SCA
Sbjct: 2    DSLLLIVLTIL-LSSVIILYR-NCRLFSILFRSSKTKTPLPLGNLGWPFLGETLEFVSCA 59

Query: 1541 YTDQPETFMDKRRRMYGKVFKSHIFGSPTIVSTDEEVSRNILQGDSNTFVPSYPKSLTIL 1362
            Y+D+PE+FMDKRRRMYGKVFKSHIFG+PTIVSTD EVS+ ILQGD+  FVPSYPKSLT L
Sbjct: 60   YSDRPESFMDKRRRMYGKVFKSHIFGTPTIVSTDAEVSKFILQGDAKMFVPSYPKSLTEL 119

Query: 1361 MGESSILLINGYLQRRIHGLIGSFLKSPNLKAQITRDMQKFVQQSMGSWDEDRLIYIQDE 1182
            MG+SSILLING LQ+RIHGLIGSF KSP+LKAQITRDMQ +VQ+SM +W  D+ IYIQDE
Sbjct: 120  MGKSSILLINGSLQKRIHGLIGSFFKSPHLKAQITRDMQVYVQESMKNWRCDQPIYIQDE 179

Query: 1181 TKKIAFQVLVKALISLDPGDEMEFLRKQFQEFIAGLMSLPINIPGTRLYRSLQAKKKMVK 1002
            TK IAFQVLVKALISLDPG EME L+KQFQEFI GLMSLP+NIPGT+LYRSLQAKKKMVK
Sbjct: 180  TKNIAFQVLVKALISLDPGQEMESLKKQFQEFICGLMSLPVNIPGTQLYRSLQAKKKMVK 239

Query: 1001 LMGKIIEAKKNGGSSKVPKDVAEVLLNE---NMKDDIISDNMIDLMIPGEDSVPVLITLA 831
            L+ +II++K++GG S VPKDV EVLLN+    + DD+I+DNMID+MIPGEDSVPVL+TLA
Sbjct: 240  LVQRIIQSKRDGGISTVPKDVVEVLLNDANAQLTDDLIADNMIDMMIPGEDSVPVLMTLA 299

Query: 830  IKYLSDCPAALQQLLEENMKLKALKDQMGEEMIWSDYLSLPFTQTVITETLRLGNIINGV 651
            +KYLSDCPAALQQL +ENMKLK+LK Q+GE + W+DYLSLPFTQ VITETLR+GNII GV
Sbjct: 300  VKYLSDCPAALQQLTDENMKLKSLKAQLGEPLTWTDYLSLPFTQRVITETLRMGNIIIGV 359

Query: 650  MRKAMKDVQLKGGYVIPKGWCVFTYFRSVHLDENLYDSPHQFNPWRWQDRDTSGCSFTPF 471
            MRKAMKD+++K GY+IPKGWC F YFRSVHLDEN YD P+QFNPWRWQD+D S  +FTPF
Sbjct: 360  MRKAMKDIEIK-GYLIPKGWCAFAYFRSVHLDENHYDWPYQFNPWRWQDKDMSNSNFTPF 418

Query: 470  GGGQRLCPGLDLARLEVSIFLHHFVTQFKWVAEDDSIVNFPTVRMKNKMPIWVKRRSSHD 291
            GGGQRLCPGLDLARLE SIFLH+FVTQF WVAE+D+IVNFPTVRMK +MPIWVKRR    
Sbjct: 419  GGGQRLCPGLDLARLEASIFLHNFVTQFSWVAEEDTIVNFPTVRMKRRMPIWVKRREEDQ 478

Query: 290  AFGL 279
            +  L
Sbjct: 479  SLSL 482


>ref|XP_002327539.1| cytochrome P450 probable 6-deoxoteasterone to 3-dehydro
            6-deoxoteasterone or teasterone to 3-dehydro teasterone
            [Populus trichocarpa] gi|222836093|gb|EEE74514.1|
            cytochrome P450 probable 6-deoxoteasterone to 3-dehydro
            6-deoxoteasterone or teasterone to 3-dehydro teasterone
            [Populus trichocarpa]
          Length = 459

 Score =  703 bits (1815), Expect = 0.0
 Identities = 347/445 (77%), Positives = 394/445 (88%), Gaps = 5/445 (1%)
 Frame = -3

Query: 1613 ETSLPLGTLGWPLVGETMEFISCAYTDQPETFMDKRRRMYGKVFKSHIFGSPTIVSTDEE 1434
            +T LPLG LGWP +GET++F+SCAY+D+PE+FMDKRRRMYGKVFKSHIFGSPTIVSTD E
Sbjct: 16   KTLLPLGALGWPFIGETIDFVSCAYSDRPESFMDKRRRMYGKVFKSHIFGSPTIVSTDAE 75

Query: 1433 VSRNILQGDSNTFVPSYPKSLTILMGESSILLINGYLQRRIHGLIGSFLKSPNLKAQITR 1254
            VS+ ILQ D+  FVPSYPKSLT LMG+SSILLING LQRRIHGLIG+F KSP+LKAQITR
Sbjct: 76   VSKFILQSDARLFVPSYPKSLTELMGKSSILLINGSLQRRIHGLIGAFFKSPHLKAQITR 135

Query: 1253 DMQKFVQQSMGSWDEDRLIYIQDETKKIAFQVLVKALISLDPGDEMEFLRKQFQEFIAGL 1074
            DMQ +VQ+SM  W ED  I+IQDETK IAFQVLVKALISLDPG+EME L+KQFQEFIAGL
Sbjct: 136  DMQSYVQESMEKWREDHPIFIQDETKNIAFQVLVKALISLDPGEEMELLKKQFQEFIAGL 195

Query: 1073 MSLPI-NIPGTRLYRSLQAKKKMVKLMGKIIEAKKNGGS-SKVPKDVAEVLLN---ENMK 909
            MSLP+ NIPG++LYRSLQAKKKMVKL+ KII++K++ G  S VPKD+ +VLLN   E + 
Sbjct: 196  MSLPLKNIPGSQLYRSLQAKKKMVKLVQKIIKSKRDHGMISMVPKDLVQVLLNDASEQLT 255

Query: 908  DDIISDNMIDLMIPGEDSVPVLITLAIKYLSDCPAALQQLLEENMKLKALKDQMGEEMIW 729
            DD+I+DNMID+MIPGEDSVPVL+TLA+KYLSDCP AL QL EENMKLK+LK Q GE M W
Sbjct: 256  DDLIADNMIDMMIPGEDSVPVLMTLAVKYLSDCPPALHQLTEENMKLKSLKAQHGEPMCW 315

Query: 728  SDYLSLPFTQTVITETLRLGNIINGVMRKAMKDVQLKGGYVIPKGWCVFTYFRSVHLDEN 549
            SDYLSLPFTQTVITETLR+GNII GVMRKAMKD+++K GY+IPKGWC F YFRSVHLDEN
Sbjct: 316  SDYLSLPFTQTVITETLRMGNIIIGVMRKAMKDIEIK-GYLIPKGWCAFAYFRSVHLDEN 374

Query: 548  LYDSPHQFNPWRWQDRDTSGCSFTPFGGGQRLCPGLDLARLEVSIFLHHFVTQFKWVAED 369
             Y+ P+QFNPWRWQD+D S  SFTPFGGGQRLCPGLDLARLE SIFLHHFVTQF+WVAED
Sbjct: 375  NYEWPYQFNPWRWQDKDMSNSSFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFRWVAED 434

Query: 368  DSIVNFPTVRMKNKMPIWVKRRSSH 294
            D+IVNFPTVRMK +MPIWVKRR  +
Sbjct: 435  DTIVNFPTVRMKRRMPIWVKRRGEN 459