BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00009458
         (1699 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002283797.1| PREDICTED: ubiquitin-like modifier-activatin...   621   e-175
ref|XP_002511184.1| Ubiquitin-activating enzyme E1 domain-contai...   610   e-172
ref|XP_003602810.1| Ubiquitin-like modifier-activating enzyme [M...   594   e-167
ref|XP_002321704.1| predicted protein [Populus trichocarpa] gi|2...   594   e-167
ref|XP_004145156.1| PREDICTED: ubiquitin-like modifier-activatin...   591   e-166

>ref|XP_002283797.1| PREDICTED: ubiquitin-like modifier-activating enzyme 5 isoform 1
            [Vitis vinifera] gi|297734421|emb|CBI15668.3| unnamed
            protein product [Vitis vinifera]
          Length = 427

 Score =  621 bits (1601), Expect = e-175
 Identities = 317/427 (74%), Positives = 345/427 (80%)
 Frame = -3

Query: 1541 MEVEIKDMXXXXXXXXXXXXXXSHQASLHKMRSHVENLAALVNSGQGRRSKVKDMSAEVV 1362
            M+VE+K+M              SHQAS+ K++ HVE+L  L      RRSKVKDMSAEVV
Sbjct: 1    MDVELKEMLDDLQALKRSLPDPSHQASIDKLQVHVEHLTNLAQYAPARRSKVKDMSAEVV 60

Query: 1361 DSNPYSRLMALQRMGIVENYERIRDYXXXXXXXXXXXXXXAEMLTRCGIGRLLLYDYDKV 1182
            DSNPYSRLMALQRMGIVENYERIR++              AEMLTRCGIGRLLLYDYDKV
Sbjct: 61   DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 1181 ELANMNRLFFRPEQCGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFMSSLTSKT 1002
            ELANMNRLFFRPEQ GMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFMSSL  KT
Sbjct: 121  ELANMNRLFFRPEQVGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFMSSLRKKT 180

Query: 1001 HNPSKKGTGVDLVLSCVDNYEARMVVNQACNELNQTWMESGVSEDAVSGHIQLLIPGETA 822
              P K+G+GVDLVLSCVDNYEARMVVNQACNELNQTWMESGVSEDAVSGHIQLLIPGETA
Sbjct: 181  FRPKKEGSGVDLVLSCVDNYEARMVVNQACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240

Query: 821  CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNTLKYLLQFGQVSPYLGY 642
            CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNTLK+LL+FG VSPYLGY
Sbjct: 241  CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY 300

Query: 641  NALKDYFPTMHMKPNPQCSNAACLERQEEYELTKPTREXXXXXXXXXXXXAVVESAIHLD 462
            NALKDYFPTM M+PNPQCSNA+CLERQ+EY L KP R+             V E  IH+D
Sbjct: 301  NALKDYFPTMEMRPNPQCSNASCLERQKEYLLAKPARDAAAKAKMEAEALVVAECPIHVD 360

Query: 461  NEWNICVVDDDEQDKKAVQKPDMLPEGLVRELPSADEYQKLTAQEESINSVDDLEDLKRQ 282
            NEWNI VVDD E D+   +  D LPEGL  ELP+ADE++K    E + NSVDDL+DL+RQ
Sbjct: 361  NEWNISVVDDSELDRTDGRSSDALPEGLTHELPTADEFEKQPTPETTFNSVDDLDDLRRQ 420

Query: 281  LEALNAE 261
            L+ALNA+
Sbjct: 421  LDALNAD 427


>ref|XP_002511184.1| Ubiquitin-activating enzyme E1 domain-containing protein, putative
            [Ricinus communis] gi|223550299|gb|EEF51786.1|
            Ubiquitin-activating enzyme E1 domain-containing protein,
            putative [Ricinus communis]
          Length = 428

 Score =  610 bits (1572), Expect = e-172
 Identities = 313/428 (73%), Positives = 340/428 (79%), Gaps = 1/428 (0%)
 Frame = -3

Query: 1541 MEVEIKDMXXXXXXXXXXXXXXSHQASLHKMRSHVENLAALVNSGQGRRSKVKDMSAEVV 1362
            ME E+K +              S QAS+HK++S VE +  L  S   RRSKVKDMSAEVV
Sbjct: 1    MEAELKGLLDDLSSLISSLPDASRQASIHKLQSRVEVITKLATSAPSRRSKVKDMSAEVV 60

Query: 1361 DSNPYSRLMALQRMGIVENYERIRDYXXXXXXXXXXXXXXAEMLTRCGIGRLLLYDYDKV 1182
            DSNPYSRLMALQRMGIV NYERIR++              AEMLTRCGIGRLLLYDYDKV
Sbjct: 61   DSNPYSRLMALQRMGIVHNYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 1181 ELANMNRLFFRPEQCGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFMSSLTSKT 1002
            ELANMNRLFFRP+Q GMTKTDAAVQTLS+INPDVVLESYT+NITTVQGFETF+SSL SK+
Sbjct: 121  ELANMNRLFFRPDQVGMTKTDAAVQTLSEINPDVVLESYTMNITTVQGFETFVSSLKSKS 180

Query: 1001 HNPSKKGTGVDLVLSCVDNYEARMVVNQACNELNQTWMESGVSEDAVSGHIQLLIPGETA 822
              PSK+GTGVDLVLSCVDNYEARM VNQACNELNQTWMESGVSEDAVSGHIQLLIPGETA
Sbjct: 181  FRPSKEGTGVDLVLSCVDNYEARMAVNQACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240

Query: 821  CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNTLKYLLQFGQVSPYLGY 642
            CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNTLK+LLQFG VSPYLGY
Sbjct: 241  CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLQFGHVSPYLGY 300

Query: 641  NALKDYFPTMHMKPNPQCSNAACLERQEEYELTKPTREXXXXXXXXXXXXAVVESAIHLD 462
            N+LKDYFPTM MKPNPQCSNAACLERQ+EY + KP R+               E  +H D
Sbjct: 301  NSLKDYFPTMEMKPNPQCSNAACLERQKEYIIAKPARDAAAKAKMEAEASLATEIPLHTD 360

Query: 461  NEWNICVVDDDE-QDKKAVQKPDMLPEGLVRELPSADEYQKLTAQEESINSVDDLEDLKR 285
            NEWNI VVDD E + K A    D LPEGL RELP+ADE+QK  A E S  + DDLE+LK+
Sbjct: 361  NEWNISVVDDSEPETKDATSSADKLPEGLARELPTADEFQKFPAAETSTTTFDDLEELKK 420

Query: 284  QLEALNAE 261
            QLEALN +
Sbjct: 421  QLEALNTD 428


>ref|XP_003602810.1| Ubiquitin-like modifier-activating enzyme [Medicago truncatula]
            gi|355491858|gb|AES73061.1| Ubiquitin-like
            modifier-activating enzyme [Medicago truncatula]
            gi|388517059|gb|AFK46591.1| unknown [Medicago truncatula]
          Length = 427

 Score =  594 bits (1532), Expect = e-167
 Identities = 301/426 (70%), Positives = 340/426 (79%)
 Frame = -3

Query: 1541 MEVEIKDMXXXXXXXXXXXXXXSHQASLHKMRSHVENLAALVNSGQGRRSKVKDMSAEVV 1362
            ME+E+ D+              S + + HK++SHVE++A LV +   RR+KVKDMSAEVV
Sbjct: 1    MEIELNDLITDLQSLNQSLPDPSLRDAFHKIQSHVEHIADLVRAQPVRRTKVKDMSAEVV 60

Query: 1361 DSNPYSRLMALQRMGIVENYERIRDYXXXXXXXXXXXXXXAEMLTRCGIGRLLLYDYDKV 1182
            DSNPYSRLMALQRMGIVENYERIRD+              AEMLTRCGIGRLLLYDYDKV
Sbjct: 61   DSNPYSRLMALQRMGIVENYERIRDFSIAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 1181 ELANMNRLFFRPEQCGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFMSSLTSKT 1002
            ELANMNRLFFRP+Q GMTKTDAA+QTLSDINPDVVLES+TLNITTV GFETFMSSL +K+
Sbjct: 121  ELANMNRLFFRPDQAGMTKTDAALQTLSDINPDVVLESFTLNITTVDGFETFMSSLKNKS 180

Query: 1001 HNPSKKGTGVDLVLSCVDNYEARMVVNQACNELNQTWMESGVSEDAVSGHIQLLIPGETA 822
              P K+G+GVDLVLSCVDNYEARM VNQACNEL+QTWMESGVSEDAVSGHIQLLIPGETA
Sbjct: 181  FRPDKQGSGVDLVLSCVDNYEARMAVNQACNELSQTWMESGVSEDAVSGHIQLLIPGETA 240

Query: 821  CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNTLKYLLQFGQVSPYLGY 642
            CFACAPPLVVASG+DERTLKREGVCAASLPTTMGV+AGLLVQNTLK+LL FGQVSPYLGY
Sbjct: 241  CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVIAGLLVQNTLKFLLGFGQVSPYLGY 300

Query: 641  NALKDYFPTMHMKPNPQCSNAACLERQEEYELTKPTREXXXXXXXXXXXXAVVESAIHLD 462
            N+LKD+FPTM MKPNPQCSNAACL+RQ EY L KPTR+             + E  +H D
Sbjct: 301  NSLKDFFPTMQMKPNPQCSNAACLKRQGEYILAKPTRDAAAKAKLEAEGPLIEEGPLHDD 360

Query: 461  NEWNICVVDDDEQDKKAVQKPDMLPEGLVRELPSADEYQKLTAQEESINSVDDLEDLKRQ 282
            NEWNI VVDD E D    +  D LPEGL  ELP+ADE+Q L  +E   N  DDL++L++Q
Sbjct: 361  NEWNISVVDDCEPDGANTKSSDALPEGLTHELPTADEFQNLVTREAPSNDNDDLDELRKQ 420

Query: 281  LEALNA 264
            LEA+N+
Sbjct: 421  LEAINS 426


>ref|XP_002321704.1| predicted protein [Populus trichocarpa] gi|222868700|gb|EEF05831.1|
            predicted protein [Populus trichocarpa]
          Length = 428

 Score =  594 bits (1531), Expect = e-167
 Identities = 306/428 (71%), Positives = 342/428 (79%), Gaps = 1/428 (0%)
 Frame = -3

Query: 1541 MEVEIKDMXXXXXXXXXXXXXXSHQAS-LHKMRSHVENLAALVNSGQGRRSKVKDMSAEV 1365
            ME E+K+M              S+ AS + K++S VE+L  L  S   RR+KV+DMSAEV
Sbjct: 1    MEAELKEMLNDLDSLKQSQPDPSNLASSILKLQSRVEHLTKLAKSAPVRRTKVQDMSAEV 60

Query: 1364 VDSNPYSRLMALQRMGIVENYERIRDYXXXXXXXXXXXXXXAEMLTRCGIGRLLLYDYDK 1185
            VDSNPYSRLMALQRMGIV+NYERIRD+              AEMLTRCGIGRLLLYDYDK
Sbjct: 61   VDSNPYSRLMALQRMGIVDNYERIRDFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDK 120

Query: 1184 VELANMNRLFFRPEQCGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFMSSLTSK 1005
            VELANMNRLFFRPEQ GMTKTDAA QTLSDINPDVVLES+TLNITTVQGFETFMSSL +K
Sbjct: 121  VELANMNRLFFRPEQVGMTKTDAASQTLSDINPDVVLESFTLNITTVQGFETFMSSLRNK 180

Query: 1004 THNPSKKGTGVDLVLSCVDNYEARMVVNQACNELNQTWMESGVSEDAVSGHIQLLIPGET 825
            +  P+K+G+GVDLVLSCVDNYEARM VNQACNELNQTWMESGVSEDAVSGHIQLLIPGET
Sbjct: 181  SFRPNKEGSGVDLVLSCVDNYEARMAVNQACNELNQTWMESGVSEDAVSGHIQLLIPGET 240

Query: 824  ACFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNTLKYLLQFGQVSPYLG 645
            ACFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNTLK LLQFGQVSPYLG
Sbjct: 241  ACFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNTLKLLLQFGQVSPYLG 300

Query: 644  YNALKDYFPTMHMKPNPQCSNAACLERQEEYELTKPTREXXXXXXXXXXXXAVVESAIHL 465
            YN+LKD+FPTM M+PNPQCSNAACLERQ+EY L KP R+            +V E  +H 
Sbjct: 301  YNSLKDFFPTMEMRPNPQCSNAACLERQKEYLLVKPARDAAIIAKMEAEALSVPEGPLHT 360

Query: 464  DNEWNICVVDDDEQDKKAVQKPDMLPEGLVRELPSADEYQKLTAQEESINSVDDLEDLKR 285
            DNEWNI VVDD   ++      D LPEGL RELP+ADE+QK  A E +  + DD+E+L++
Sbjct: 361  DNEWNISVVDDSAPERTDATSSDALPEGLTRELPAADEFQKFPAAEPATATFDDIEELRK 420

Query: 284  QLEALNAE 261
            QL+ALNA+
Sbjct: 421  QLDALNAD 428


>ref|XP_004145156.1| PREDICTED: ubiquitin-like modifier-activating enzyme 5-like [Cucumis
            sativus] gi|449474254|ref|XP_004154119.1| PREDICTED:
            ubiquitin-like modifier-activating enzyme 5-like [Cucumis
            sativus] gi|449503682|ref|XP_004162124.1| PREDICTED:
            ubiquitin-like modifier-activating enzyme 5-like [Cucumis
            sativus]
          Length = 426

 Score =  591 bits (1524), Expect = e-166
 Identities = 306/426 (71%), Positives = 336/426 (78%)
 Frame = -3

Query: 1541 MEVEIKDMXXXXXXXXXXXXXXSHQASLHKMRSHVENLAALVNSGQGRRSKVKDMSAEVV 1362
            ME E+KDM              S +  + K++ HVE L  L  S   RRSKVKDMSAEVV
Sbjct: 1    MEAELKDMWNDLESLKHSLTDPSLRGPVDKLQLHVERLTNLSKSVPVRRSKVKDMSAEVV 60

Query: 1361 DSNPYSRLMALQRMGIVENYERIRDYXXXXXXXXXXXXXXAEMLTRCGIGRLLLYDYDKV 1182
            DSNPYSRLMALQRMGIVENYERIR++              AEMLTRCGIGRLLLYDYDKV
Sbjct: 61   DSNPYSRLMALQRMGIVENYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDKV 120

Query: 1181 ELANMNRLFFRPEQCGMTKTDAAVQTLSDINPDVVLESYTLNITTVQGFETFMSSLTSKT 1002
            ELANMNRLFFRPEQ GMTKTDAAVQTL+DINPDVVLESYT NITTVQGFETFMSSL +K+
Sbjct: 121  ELANMNRLFFRPEQVGMTKTDAAVQTLADINPDVVLESYTFNITTVQGFETFMSSLRNKS 180

Query: 1001 HNPSKKGTGVDLVLSCVDNYEARMVVNQACNELNQTWMESGVSEDAVSGHIQLLIPGETA 822
             +P+K+G+GVDLVLSCVDNYEARM VNQACNELNQTWMESGVSEDAVSGHIQLLIPGETA
Sbjct: 181  FSPTKEGSGVDLVLSCVDNYEARMAVNQACNELNQTWMESGVSEDAVSGHIQLLIPGETA 240

Query: 821  CFACAPPLVVASGVDERTLKREGVCAASLPTTMGVVAGLLVQNTLKYLLQFGQVSPYLGY 642
            CFACAPPLVVASG+DERTLKREGVCAASLPTTMGVVAGLLVQNTLK+LL+FG VSPYLGY
Sbjct: 241  CFACAPPLVVASGIDERTLKREGVCAASLPTTMGVVAGLLVQNTLKFLLKFGHVSPYLGY 300

Query: 641  NALKDYFPTMHMKPNPQCSNAACLERQEEYELTKPTREXXXXXXXXXXXXAVVESAIHLD 462
            N+LKDYFPTM M+PNPQCSN ACLERQ+E+ + KP R+             V E  +H D
Sbjct: 301  NSLKDYFPTMEMRPNPQCSNVACLERQKEFIIAKPARDAAAMAKMEAEALTVEEIPLHAD 360

Query: 461  NEWNICVVDDDEQDKKAVQKPDMLPEGLVRELPSADEYQKLTAQEESINSVDDLEDLKRQ 282
            NEWNI VVD+ E +       D LPEGLVRELP+ADE Q     E +  S+DDLEDLKRQ
Sbjct: 361  NEWNISVVDEVEIETVGATSSDALPEGLVRELPNADESQHPPPAEPAAPSLDDLEDLKRQ 420

Query: 281  LEALNA 264
            LEALN+
Sbjct: 421  LEALNS 426