BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00009451
         (1275 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002284045.1| PREDICTED: endoplasmic reticulum-Golgi inter...   605   e-171
ref|XP_003555674.1| PREDICTED: endoplasmic reticulum-Golgi inter...   587   e-165
gb|ACU19028.1| unknown [Glycine max]                                  585   e-165
ref|XP_002322569.1| predicted protein [Populus trichocarpa] gi|2...   585   e-164
ref|XP_003542168.1| PREDICTED: probable endoplasmic reticulum-Go...   583   e-164

>ref|XP_002284045.1| PREDICTED: endoplasmic reticulum-Golgi intermediate compartment
            protein 3 [Vitis vinifera] gi|296086333|emb|CBI31774.3|
            unnamed protein product [Vitis vinifera]
          Length = 351

 Score =  605 bits (1560), Expect = e-171
 Identities = 289/351 (82%), Positives = 319/351 (90%), Gaps = 2/351 (0%)
 Frame = -1

Query: 1074 MGVKQAIRNLDAFPRAEDHLMQKTQSGAIVSVVGLVIMTTLFVHELRYYLTTYTVHQMAV 895
            MGVKQ I++L AFPRAE+HL+QKTQSGA+VS++GLVIM TLF+HELRYYLTTYTVHQM+V
Sbjct: 1    MGVKQFIKSLHAFPRAEEHLLQKTQSGAVVSIIGLVIMATLFLHELRYYLTTYTVHQMSV 60

Query: 894  DLKRGETLPIHINMTFPSLPCDVISVDAIDMSGKHEVDLDTNIWKLRLNKDGYIIGTEYV 715
            DLKRGETLPIHINMTFPSLPCDV+SVDAIDMSGKHEVDLDTNIWKLRLN+DG+IIGTEY+
Sbjct: 61   DLKRGETLPIHINMTFPSLPCDVLSVDAIDMSGKHEVDLDTNIWKLRLNRDGFIIGTEYL 120

Query: 714  SDLVEKGHTSHKHADDKDRH--EDLKLHLDAFDQGGEDTIKNVKLALKSGEGCRVYGVLD 541
            SDLVEK H  HKH  +KD H   D KLH  +FDQ  E+ +K VK AL +GEGCRVYGVLD
Sbjct: 121  SDLVEKEHADHKHDHNKDHHGDSDQKLHAHSFDQDAENMVKKVKQALANGEGCRVYGVLD 180

Query: 540  VQRVAGNFHISVHGLNIFVAQMIFEGATHVNVSHIIHDLSFGPKYPGIHNPLDGTSRIIQ 361
            VQRVAGNFHISVHGLNIFVAQMIF+GA HVNVSHIIHDLSFGPKYPG+HNPLDGT RI++
Sbjct: 181  VQRVAGNFHISVHGLNIFVAQMIFDGAIHVNVSHIIHDLSFGPKYPGLHNPLDGTVRILR 240

Query: 360  KGSGTFKYYIKVVPTEYNYLSKELLPTNQFSVTEYFSPMNEFDRTWPAVYFLYDLSPITV 181
              SGTFKYYIK+VPTEY Y+SKE+LPTNQFSV EYFSPMNEFDRTWPAVYFLYDLSP+TV
Sbjct: 241  GASGTFKYYIKIVPTEYRYISKEVLPTNQFSVMEYFSPMNEFDRTWPAVYFLYDLSPVTV 300

Query: 180  TIKEERRSFLHFITRLSAVLGGTFAVTGMLDKWMYKLVEALVKPNAKSGYR 28
            TIKEERRSFLHFITRL AVLGGTFA+TGMLD+WMY+ +E L KPNAKS YR
Sbjct: 301  TIKEERRSFLHFITRLCAVLGGTFALTGMLDRWMYRFLEMLTKPNAKSVYR 351


>ref|XP_003555674.1| PREDICTED: endoplasmic reticulum-Golgi intermediate compartment
            protein 3-like [Glycine max]
          Length = 347

 Score =  587 bits (1512), Expect = e-165
 Identities = 281/346 (81%), Positives = 314/346 (90%), Gaps = 1/346 (0%)
 Frame = -1

Query: 1074 MGVKQAIRNLDAFPRAEDHLMQKTQSGAIVSVVGLVIMTTLFVHELRYYLTTYTVHQMAV 895
            MG+KQ I+NLDAFPRAEDHL+QKTQSGA+VSV+GL+IM TLFVHEL YYLTTYTVHQM+V
Sbjct: 1    MGMKQVIKNLDAFPRAEDHLLQKTQSGALVSVIGLIIMATLFVHELGYYLTTYTVHQMSV 60

Query: 894  DLKRGETLPIHINMTFPSLPCDVISVDAIDMSGKHEVDLDTNIWKLRLNKDGYIIGTEYV 715
            DLKRGETLPIHINMTFPSLPCDV+SVDAIDMSGKHEVDLDTNIWKLRLN  G+IIGTEY+
Sbjct: 61   DLKRGETLPIHINMTFPSLPCDVLSVDAIDMSGKHEVDLDTNIWKLRLNSYGHIIGTEYI 120

Query: 714  SDLVEKGHTSHKHADDKDR-HEDLKLHLDAFDQGGEDTIKNVKLALKSGEGCRVYGVLDV 538
            SDLVEK HT HKH D+K+  H + K+HL   D+  E+ IK VK ALK+GEGCRVYGVLDV
Sbjct: 121  SDLVEKEHTHHKHDDNKNHEHSEQKIHLQNLDESTENIIKKVKEALKNGEGCRVYGVLDV 180

Query: 537  QRVAGNFHISVHGLNIFVAQMIFEGATHVNVSHIIHDLSFGPKYPGIHNPLDGTSRIIQK 358
            QRVAGNFHISVHGLNI+VAQMIF+GA +VNVSH IHDLSFGPKYPG+HNPLD T+RI+  
Sbjct: 181  QRVAGNFHISVHGLNIYVAQMIFDGAKNVNVSHFIHDLSFGPKYPGLHNPLDDTTRILHD 240

Query: 357  GSGTFKYYIKVVPTEYNYLSKELLPTNQFSVTEYFSPMNEFDRTWPAVYFLYDLSPITVT 178
             SGTFKYYIKVVPTEY Y+SKE+LPTNQFSV+EY+SP+N+FDRTWPAVYFLYDLSPITVT
Sbjct: 241  TSGTFKYYIKVVPTEYRYISKEVLPTNQFSVSEYYSPINQFDRTWPAVYFLYDLSPITVT 300

Query: 177  IKEERRSFLHFITRLSAVLGGTFAVTGMLDKWMYKLVEALVKPNAK 40
            IKEERRSFLHFITRL AVLGGTFAVTGMLD+WMY+L+E L K  +K
Sbjct: 301  IKEERRSFLHFITRLCAVLGGTFAVTGMLDRWMYRLLETLTKSKSK 346


>gb|ACU19028.1| unknown [Glycine max]
          Length = 347

 Score =  585 bits (1509), Expect = e-165
 Identities = 281/346 (81%), Positives = 313/346 (90%), Gaps = 1/346 (0%)
 Frame = -1

Query: 1074 MGVKQAIRNLDAFPRAEDHLMQKTQSGAIVSVVGLVIMTTLFVHELRYYLTTYTVHQMAV 895
            MG+KQ I+NLDAFPRAEDHL+QKTQSGA+VSV+GL+IM TLFVHEL YYLTTYTVHQM+V
Sbjct: 1    MGMKQVIKNLDAFPRAEDHLLQKTQSGALVSVIGLIIMATLFVHELGYYLTTYTVHQMSV 60

Query: 894  DLKRGETLPIHINMTFPSLPCDVISVDAIDMSGKHEVDLDTNIWKLRLNKDGYIIGTEYV 715
            DLKRGETLPIHINMTFPSLPCDV+SVDAIDMSGKHEVDLDTNIWKLRLN  G+IIGTEYV
Sbjct: 61   DLKRGETLPIHINMTFPSLPCDVLSVDAIDMSGKHEVDLDTNIWKLRLNSYGHIIGTEYV 120

Query: 714  SDLVEKGHTSHKHADDKDR-HEDLKLHLDAFDQGGEDTIKNVKLALKSGEGCRVYGVLDV 538
            SDLVEK HT HKH D+K+  H + K+HL   D+  E+ IK VK ALK+GEGCRVYGVLDV
Sbjct: 121  SDLVEKEHTHHKHDDNKNHEHSEQKIHLQNLDESTENIIKKVKEALKNGEGCRVYGVLDV 180

Query: 537  QRVAGNFHISVHGLNIFVAQMIFEGATHVNVSHIIHDLSFGPKYPGIHNPLDGTSRIIQK 358
            QRVAGNFHISVHGLNI+VAQMIF+GA +VNVSH IHDLSFGPKYPG+HNPLD T+RI+  
Sbjct: 181  QRVAGNFHISVHGLNIYVAQMIFDGAKNVNVSHFIHDLSFGPKYPGLHNPLDDTTRILHD 240

Query: 357  GSGTFKYYIKVVPTEYNYLSKELLPTNQFSVTEYFSPMNEFDRTWPAVYFLYDLSPITVT 178
             SGTFKYYIKVVPTEY Y+SKE+LPTNQFSV+EY+SP+N+FDRTWPAVYFLYDLSPITVT
Sbjct: 241  TSGTFKYYIKVVPTEYRYISKEVLPTNQFSVSEYYSPINQFDRTWPAVYFLYDLSPITVT 300

Query: 177  IKEERRSFLHFITRLSAVLGGTFAVTGMLDKWMYKLVEALVKPNAK 40
            IKEERRSF HFITRL AVLGGTFAVTGMLD+WMY+L+E L K  +K
Sbjct: 301  IKEERRSFFHFITRLCAVLGGTFAVTGMLDRWMYRLLETLTKSKSK 346


>ref|XP_002322569.1| predicted protein [Populus trichocarpa] gi|222867199|gb|EEF04330.1|
            predicted protein [Populus trichocarpa]
          Length = 351

 Score =  585 bits (1507), Expect = e-164
 Identities = 279/350 (79%), Positives = 313/350 (89%), Gaps = 2/350 (0%)
 Frame = -1

Query: 1074 MGVKQAIRNLDAFPRAEDHLMQKTQSGAIVSVVGLVIMTTLFVHELRYYLTTYTVHQMAV 895
            MGVKQAI++LDAFPRAE+HL+QKTQSGA+VSV+GLVIM TLF HEL YYLTTYTVHQM+V
Sbjct: 1    MGVKQAIKSLDAFPRAEEHLLQKTQSGALVSVIGLVIMATLFYHELAYYLTTYTVHQMSV 60

Query: 894  DLKRGETLPIHINMTFPSLPCDVISVDAIDMSGKHEVDLDTNIWKLRLNKDGYIIGTEYV 715
            DL+RGE LPIH+N+TFPSLPCDV+SVDAIDMSGKHEVDLDTNIWKLRLN  G+I GTEY+
Sbjct: 61   DLQRGEILPIHVNITFPSLPCDVLSVDAIDMSGKHEVDLDTNIWKLRLNSHGHITGTEYL 120

Query: 714  SDLVEKGHTSHKHADDKDRHEDL--KLHLDAFDQGGEDTIKNVKLALKSGEGCRVYGVLD 541
            SDLVEK H +H H  DKD H+D   + H   FD   E  IK VK AL +GEGCRVYGVLD
Sbjct: 121  SDLVEKEHEAHNHDHDKDHHKDSHEEQHTHGFDDAAETMIKKVKQALANGEGCRVYGVLD 180

Query: 540  VQRVAGNFHISVHGLNIFVAQMIFEGATHVNVSHIIHDLSFGPKYPGIHNPLDGTSRIIQ 361
            VQRVAGNFHISVHGLNIFVAQMIF+GA HVNVSHIIHDLSFGPKYPGIHNPLDGT+RI++
Sbjct: 181  VQRVAGNFHISVHGLNIFVAQMIFDGAKHVNVSHIIHDLSFGPKYPGIHNPLDGTARILR 240

Query: 360  KGSGTFKYYIKVVPTEYNYLSKELLPTNQFSVTEYFSPMNEFDRTWPAVYFLYDLSPITV 181
            + SG FKYYIK+VPTEY Y+SK++LPTNQFSVTEYFSP+ +FDRTWPAVYFLYDLSPITV
Sbjct: 241  ETSGIFKYYIKIVPTEYRYISKDVLPTNQFSVTEYFSPITDFDRTWPAVYFLYDLSPITV 300

Query: 180  TIKEERRSFLHFITRLSAVLGGTFAVTGMLDKWMYKLVEALVKPNAKSGY 31
            TIKEERRSFLHFITRL A+LGGTFA+TGMLD+WMY+L+EAL KPN  SG+
Sbjct: 301  TIKEERRSFLHFITRLCAILGGTFALTGMLDRWMYRLLEALTKPNRGSGF 350


>ref|XP_003542168.1| PREDICTED: probable endoplasmic reticulum-Golgi intermediate
            compartment protein 3-like [Glycine max]
          Length = 351

 Score =  583 bits (1503), Expect = e-164
 Identities = 280/350 (80%), Positives = 315/350 (90%), Gaps = 5/350 (1%)
 Frame = -1

Query: 1074 MGVKQAIRNLDAFPRAEDHLMQKTQSGAIVSVVGLVIMTTLFVHELRYYLTTYTVHQMAV 895
            MG+KQ I+NLDAFPRAEDHL+QKTQSGA+VSV+GL+IM TLFVHEL YYLTTYTVH+M+V
Sbjct: 1    MGMKQVIKNLDAFPRAEDHLLQKTQSGALVSVIGLIIMATLFVHELGYYLTTYTVHKMSV 60

Query: 894  DLKRGETLPIHINMTFPSLPCDVISVDAIDMSGKHEVDLDTNIWKLRLNKDGYIIGTEYV 715
            DLKRGETLPIHINMTFPSLPCDV+SVDAIDMSGKHEVDLDTNIWKLRLN  G+IIGTEY+
Sbjct: 61   DLKRGETLPIHINMTFPSLPCDVLSVDAIDMSGKHEVDLDTNIWKLRLNSYGHIIGTEYI 120

Query: 714  SDLVEKGHTSHKHADDKDR-----HEDLKLHLDAFDQGGEDTIKNVKLALKSGEGCRVYG 550
            SDLVEK HT+ +H D+KD      H + K+HL   D+  E+ IK VK ALK+GEGCRVYG
Sbjct: 121  SDLVEKEHTNQEHDDNKDHDHHHEHSEQKIHLQNLDESTENIIKKVKEALKNGEGCRVYG 180

Query: 549  VLDVQRVAGNFHISVHGLNIFVAQMIFEGATHVNVSHIIHDLSFGPKYPGIHNPLDGTSR 370
            VLDVQRVAGNFHISVHGLNI+VAQMIF+GA +VNVSH IHDLSFGPKYPG+HNPLD T+R
Sbjct: 181  VLDVQRVAGNFHISVHGLNIYVAQMIFDGAKNVNVSHFIHDLSFGPKYPGLHNPLDDTTR 240

Query: 369  IIQKGSGTFKYYIKVVPTEYNYLSKELLPTNQFSVTEYFSPMNEFDRTWPAVYFLYDLSP 190
            I+   SGTFKYYIKVVPTEY Y+SKE+LPTNQFSV+EY+SP+N+FDRTWPAVYFLYDLSP
Sbjct: 241  ILHDTSGTFKYYIKVVPTEYRYISKEVLPTNQFSVSEYYSPINQFDRTWPAVYFLYDLSP 300

Query: 189  ITVTIKEERRSFLHFITRLSAVLGGTFAVTGMLDKWMYKLVEALVKPNAK 40
            ITVTIKEERRSFLHFITRL AVLGGTFAVTGMLD+WMY+L+EAL K  +K
Sbjct: 301  ITVTIKEERRSFLHFITRLCAVLGGTFAVTGMLDRWMYRLLEALTKSKSK 350