BLASTX nr result
ID: Bupleurum21_contig00009437
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00009437
(1881 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002283154.1| PREDICTED: glutamyl-tRNA synthetase, chlorop... 860 0.0
ref|XP_002313571.1| predicted protein [Populus trichocarpa] gi|2... 846 0.0
ref|XP_002532486.1| glutamyl-tRNA synthetase 1, 2, putative [Ric... 842 0.0
sp|Q43794.1|SYE_TOBAC RecName: Full=Glutamate--tRNA ligase, chlo... 839 0.0
gb|AAY18610.1| glutamyl-tRNA synthetase [Nicotiana benthamiana] 838 0.0
>ref|XP_002283154.1| PREDICTED: glutamyl-tRNA synthetase, chloroplastic/mitochondrial
[Vitis vinifera] gi|297744457|emb|CBI37719.3| unnamed
protein product [Vitis vinifera]
Length = 566
Score = 860 bits (2222), Expect = 0.0
Identities = 435/584 (74%), Positives = 484/584 (82%)
Frame = -1
Query: 1791 MANLLDRMPWMRLRGRVLGAAPPFFHQSFSLSPSLVHCCNNHHIICLPARSKRCLSIITA 1612
MA+L+ PWM++R + APP +S SL +R S+ +
Sbjct: 1 MASLVGS-PWMKIRV-IPEVAPPILRRSSSLF-------------------RRSFSVSCS 39
Query: 1611 ATSSVEKEQDDNIVRVRFAPSPTGNLHVGGARTALFNYLFARSKGGKFVLRIEDTDLERS 1432
+ +K + + VRVRFAPSPTGNLHVGGARTALFNYLFARSKGGKFVLRIEDTDLERS
Sbjct: 40 TEAPPKKLEGE--VRVRFAPSPTGNLHVGGARTALFNYLFARSKGGKFVLRIEDTDLERS 97
Query: 1431 TKESEEAVLQDLSWLGLHWDEGPDVGGDYGPYRQSERNMLYKQYAEELLNSGHAYRCFCS 1252
TK+SEEA+LQDLSWLGLHWDEGP VGGDYGPYRQSERN LYKQYAE+LL SGH Y+CFCS
Sbjct: 98 TKQSEEALLQDLSWLGLHWDEGPGVGGDYGPYRQSERNSLYKQYAEKLLESGHVYQCFCS 157
Query: 1251 NEELEKMKEIAKLKQLPPVYTGKWASATNEEVQEQLRKGTPFTYRFRVPKDGKLKINDLI 1072
NEELEKMKEIAKLKQLPPVYTGKWASAT EEVQE+L +GTP+TYRFRVPK+G LKI+DLI
Sbjct: 158 NEELEKMKEIAKLKQLPPVYTGKWASATAEEVQEELERGTPYTYRFRVPKEGSLKIDDLI 217
Query: 1071 RGEVSWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQALIYKA 892
RGEV WNLDTLGDFVIMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQAL+YKA
Sbjct: 218 RGEVRWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQALLYKA 277
Query: 891 LGFRMPYFAHVSLILAPDRSKLSKRHGATSVGQFKEMGYLPQAVVNYLALLGWGDGTEDE 712
LGF MP FAHVSLILAPDRSKLSKRHGATSVGQFKEMG+LPQA+VNYLALLGWGDGTE+E
Sbjct: 278 LGFAMPSFAHVSLILAPDRSKLSKRHGATSVGQFKEMGFLPQAMVNYLALLGWGDGTENE 337
Query: 711 FYTLEQLVEKFSIERVNKSGAVFDSTKFRWMNGQHXXXXXXXXXXXXLGHHWKSSGILRV 532
F+TL+ LVEKFSI+RVNK GA+FDSTK RWMNGQH +G WK++GIL
Sbjct: 338 FFTLKHLVEKFSIDRVNKGGAIFDSTKLRWMNGQHLRSLPSEELTKLIGERWKNTGILTQ 397
Query: 531 SEGLFVEEAVELLKDAVYLITDSEKALLNLLSYPLYETLSSSEANPVLEDSLSEVADSLL 352
S+G F+EEAV LLKD + LITDS+KAL NLLSYPLY TL+S E PVLED LSEV+ SLL
Sbjct: 398 SDGPFIEEAVALLKDGIDLITDSDKALSNLLSYPLYATLTSPEGKPVLEDKLSEVSSSLL 457
Query: 351 AAYDSGELFNALDQGKPGWQKWVKSFGKSVKRKGKSLFMPLRVLLTGKLHGPDIGASVVL 172
AAYDSGEL AL++G GWQKWVKSFGKS+KRKGKSLFMPLRVLLTGKLHGPD+GASV+L
Sbjct: 458 AAYDSGELLQALEEGPAGWQKWVKSFGKSLKRKGKSLFMPLRVLLTGKLHGPDMGASVLL 517
Query: 171 LHSLGTGDIVASHTGFVTLEERFKLLREVEWRSFNDNQTALESA 40
LH GT +VA GFVTLE+RFK+LREVEW + N +Q LESA
Sbjct: 518 LHRAGTCGVVAPQVGFVTLEDRFKMLREVEWEALNKDQPPLESA 561
>ref|XP_002313571.1| predicted protein [Populus trichocarpa] gi|222849979|gb|EEE87526.1|
predicted protein [Populus trichocarpa]
Length = 574
Score = 846 bits (2185), Expect = 0.0
Identities = 429/588 (72%), Positives = 485/588 (82%), Gaps = 1/588 (0%)
Frame = -1
Query: 1791 MAN-LLDRMPWMRLRGRVLGAAPPFFHQSFSLSPSLVHCCNNHHIICLPARSKRCLSIIT 1615
MAN ++ PWMR+R P SF + S N+ P R + +S I
Sbjct: 1 MANSIIAGTPWMRIR-----VIPEI---SFPILRSSSSLYNHKFSFLFPTRRRFSVSAI- 51
Query: 1614 AATSSVEKEQDDNIVRVRFAPSPTGNLHVGGARTALFNYLFARSKGGKFVLRIEDTDLER 1435
+S EKEQ VRVRFAPSPTGNLHVGGARTALFNYLFARSKGGKFVLRIEDTDLER
Sbjct: 52 ---ASTEKEQ----VRVRFAPSPTGNLHVGGARTALFNYLFARSKGGKFVLRIEDTDLER 104
Query: 1434 STKESEEAVLQDLSWLGLHWDEGPDVGGDYGPYRQSERNMLYKQYAEELLNSGHAYRCFC 1255
STKESEEAVL+DLSWLGL WDEGP VGGDYGPYRQSERN LYKQ+AE+L+ SGH YRCFC
Sbjct: 105 STKESEEAVLRDLSWLGLDWDEGPGVGGDYGPYRQSERNSLYKQHAEKLVESGHVYRCFC 164
Query: 1254 SNEELEKMKEIAKLKQLPPVYTGKWASATNEEVQEQLRKGTPFTYRFRVPKDGKLKINDL 1075
SNEELE+MKEIAKLKQLPPVYTGKWA+A ++EVQE+L KGTP+TYRFRVPK+G+L INDL
Sbjct: 165 SNEELEQMKEIAKLKQLPPVYTGKWANAMDDEVQEELAKGTPYTYRFRVPKEGRLTINDL 224
Query: 1074 IRGEVSWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQALIYK 895
IRGEVSWN DTLGDFVIMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQALIYK
Sbjct: 225 IRGEVSWNYDTLGDFVIMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQALIYK 284
Query: 894 ALGFRMPYFAHVSLILAPDRSKLSKRHGATSVGQFKEMGYLPQAVVNYLALLGWGDGTED 715
ALGF MP+FAHVSLILAPDRSKLSKRHGATSVGQ++EMGYLP+A+VNYLALLGWGDGTE+
Sbjct: 285 ALGFPMPHFAHVSLILAPDRSKLSKRHGATSVGQYREMGYLPRAMVNYLALLGWGDGTEN 344
Query: 714 EFYTLEQLVEKFSIERVNKSGAVFDSTKFRWMNGQHXXXXXXXXXXXXLGHHWKSSGILR 535
EF+TLEQLVEKFSI RVNKSGAVFDSTK RWMNGQH +G WKSSGIL
Sbjct: 345 EFFTLEQLVEKFSIGRVNKSGAVFDSTKLRWMNGQHLRALPSDKLNKLVGERWKSSGILV 404
Query: 534 VSEGLFVEEAVELLKDAVYLITDSEKALLNLLSYPLYETLSSSEANPVLEDSLSEVADSL 355
SEG F+EEAV LLKD + L+TDS+K L NLLSYPL+ TLSS E ++++ LSEV+ L
Sbjct: 405 ESEGPFIEEAVLLLKDGIELVTDSDKVLSNLLSYPLHATLSSPEGKVMIQNKLSEVSSGL 464
Query: 354 LAAYDSGELFNALDQGKPGWQKWVKSFGKSVKRKGKSLFMPLRVLLTGKLHGPDIGASVV 175
LAAYDSGEL NAL++G GWQKWVK+FGK++K KGKSLFMPLRVLLTGKLHGPD+G+S++
Sbjct: 465 LAAYDSGELLNALEEGHSGWQKWVKNFGKTMKLKGKSLFMPLRVLLTGKLHGPDMGSSII 524
Query: 174 LLHSLGTGDIVASHTGFVTLEERFKLLREVEWRSFNDNQTALESATIS 31
LLH GTGD+V+S FVTL+ERF +LRE++W + N +Q LE A I+
Sbjct: 525 LLHQAGTGDMVSSQAEFVTLKERFGVLREIDWEALNKDQPPLEPAAIA 572
>ref|XP_002532486.1| glutamyl-tRNA synthetase 1, 2, putative [Ricinus communis]
gi|223527811|gb|EEF29910.1| glutamyl-tRNA synthetase 1,
2, putative [Ricinus communis]
Length = 577
Score = 842 bits (2175), Expect = 0.0
Identities = 427/589 (72%), Positives = 483/589 (82%), Gaps = 1/589 (0%)
Frame = -1
Query: 1791 MANLLDRMPWMRLRGRVLGAAPPFFHQSFSLSPSLVHCCNNHHIICLPARSKRCLSIITA 1612
MA ++ PW+R+R + APP S + NH + +R + +
Sbjct: 1 MATVIGGTPWIRMRV-IPDLAPPMSIWCPSFFVYKYNHIQNHQL-------RRSRNFSVS 52
Query: 1611 ATSSVEKEQDDNIVRVRFAPSPTGNLHVGGARTALFNYLFARSKGGKFVLRIEDTDLERS 1432
A E+ Q VRVRFAPSPTGNLHVGGARTALFNYLFARSKGGKFVLRIEDTDLERS
Sbjct: 53 AGGGGEEGQ----VRVRFAPSPTGNLHVGGARTALFNYLFARSKGGKFVLRIEDTDLERS 108
Query: 1431 TKESEEAVLQDLSWLGLHWDEGPDVGGDYGPYRQSERNMLYKQYAEELLNSGHAYRCFCS 1252
TKESE+A+L+DL+WLGL WDEGP VGGDYGPYRQSERN LYKQYA++LLNS H YRCFCS
Sbjct: 109 TKESEDALLRDLTWLGLDWDEGPHVGGDYGPYRQSERNSLYKQYADKLLNSAHVYRCFCS 168
Query: 1251 NEELEKMKEIAKLKQLPPVYTGKWASATNEEVQEQLRKGTPFTYRFRVPKDGKLKINDLI 1072
NEELEKMKEIAKLKQLPPVY+GKWA+AT+EEVQE+L K TP+TYRFRVPK+G LKINDLI
Sbjct: 169 NEELEKMKEIAKLKQLPPVYSGKWATATDEEVQEELAKATPYTYRFRVPKEGSLKINDLI 228
Query: 1071 RGEVSWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQALIYKA 892
RGEVSWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQALIYKA
Sbjct: 229 RGEVSWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQALIYKA 288
Query: 891 LGFRMPYFAHVSLILAPDRSKLSKRHGATSVGQFKEMGYLPQAVVNYLALLGWGDGTEDE 712
LGF MP FAHVSLILAPDRSKLSKRHGATSVGQF+EMGYLPQA+VNYLALLGWGDGTE+E
Sbjct: 289 LGFPMPLFAHVSLILAPDRSKLSKRHGATSVGQFREMGYLPQAMVNYLALLGWGDGTENE 348
Query: 711 FYTLEQLVEKFSIERVNKSGAVFDSTKFRWMNGQHXXXXXXXXXXXXLGHHWKSSGILRV 532
F+TL+QLVEKFSI RVNKSGA+FDSTK RWMNGQH +G WKS GIL
Sbjct: 349 FFTLDQLVEKFSIGRVNKSGAIFDSTKLRWMNGQHLRALSSEKLINLIGERWKSIGILVE 408
Query: 531 SEGLFVEEAVELLKDAVYLITDSEKALLNLLSYPLYETLSSSEANPVLEDSLSEVADSLL 352
SEG F+EEAV+LLKD + L+TDS+KAL NLLSYPL+ TL S E V+ED L EV+ SLL
Sbjct: 409 SEGSFIEEAVQLLKDGIDLVTDSDKALSNLLSYPLHATLVSPEGKTVVEDKLFEVSSSLL 468
Query: 351 AAYDSGELFNALDQGKPGWQKWVKSFGKSVKRKGKSLFMPLRVLLTGKLHGPDIGASVVL 172
AAYDSGEL +AL++G GWQKWVK+FGKS+KRKGKSLFMPLR+LLTGKLHGPD+GASV+L
Sbjct: 469 AAYDSGELLSALEEGPSGWQKWVKNFGKSMKRKGKSLFMPLRLLLTGKLHGPDMGASVIL 528
Query: 171 LHSLGTGDIVASHTGFVTLEERFKLLREVEWRSFNDNQTALE-SATISH 28
LH G IV+ GFVTL ERF++LR+V+W + N +Q LE +AT+S+
Sbjct: 529 LHKAGNCGIVSPQAGFVTLSERFEMLRQVDWEALNKDQPPLEPAATVSN 577
>sp|Q43794.1|SYE_TOBAC RecName: Full=Glutamate--tRNA ligase, chloroplastic/mitochondrial;
AltName: Full=Glutamyl-tRNA synthetase; Short=GluRS;
Flags: Precursor gi|603867|emb|CAA58506.1|
glutamate--tRNA ligase [Nicotiana tabacum]
Length = 569
Score = 839 bits (2167), Expect = 0.0
Identities = 417/560 (74%), Positives = 467/560 (83%), Gaps = 4/560 (0%)
Frame = -1
Query: 1734 AAPPFFH----QSFSLSPSLVHCCNNHHIICLPARSKRCLSIITAATSSVEKEQDDNIVR 1567
AA P+F S SL++C NH +R S+ +A D VR
Sbjct: 5 AAAPWFRVRLIPELKNSQSLLYCRGNHSYRQSLCSRRRSFSVYASAG-------DGGDVR 57
Query: 1566 VRFAPSPTGNLHVGGARTALFNYLFARSKGGKFVLRIEDTDLERSTKESEEAVLQDLSWL 1387
VRFAPSPTGNLHVGGARTALFNYL+AR+KGGKF+LRIEDTDLERSTKESEEAVL+DLSWL
Sbjct: 58 VRFAPSPTGNLHVGGARTALFNYLYARAKGGKFILRIEDTDLERSTKESEEAVLRDLSWL 117
Query: 1386 GLHWDEGPDVGGDYGPYRQSERNMLYKQYAEELLNSGHAYRCFCSNEELEKMKEIAKLKQ 1207
G WDEGP +GG+YGPYRQSERN LYKQ+AE+LL SGH YRCFCSNEELEKMKEIAKLKQ
Sbjct: 118 GPAWDEGPGIGGEYGPYRQSERNALYKQFAEKLLQSGHVYRCFCSNEELEKMKEIAKLKQ 177
Query: 1206 LPPVYTGKWASATNEEVQEQLRKGTPFTYRFRVPKDGKLKINDLIRGEVSWNLDTLGDFV 1027
LPPVYTG+WASAT EEV E+L KGTP+TYRFRVPK+G LKI+DLIRGEVSWNLDTLGDFV
Sbjct: 178 LPPVYTGRWASATEEEVVEELAKGTPYTYRFRVPKEGSLKIDDLIRGEVSWNLDTLGDFV 237
Query: 1026 IMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQALIYKALGFRMPYFAHVSLIL 847
IMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQALIYKALGF MP+FAHVSLIL
Sbjct: 238 IMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQALIYKALGFPMPHFAHVSLIL 297
Query: 846 APDRSKLSKRHGATSVGQFKEMGYLPQAVVNYLALLGWGDGTEDEFYTLEQLVEKFSIER 667
APDRSKLSKRHGATSVGQF++MGYLPQA+VNYLALLGWGDGTE+EF+TLEQLVEKF+IER
Sbjct: 298 APDRSKLSKRHGATSVGQFRDMGYLPQAMVNYLALLGWGDGTENEFFTLEQLVEKFTIER 357
Query: 666 VNKSGAVFDSTKFRWMNGQHXXXXXXXXXXXXLGHHWKSSGILRVSEGLFVEEAVELLKD 487
VNKSGA+FDSTK RWMNGQH +G WK +GI S+G+F+++AV LLKD
Sbjct: 358 VNKSGAIFDSTKLRWMNGQHLRSLPSEELNRIIGERWKDAGIATESQGIFIQDAVLLLKD 417
Query: 486 AVYLITDSEKALLNLLSYPLYETLSSSEANPVLEDSLSEVADSLLAAYDSGELFNALDQG 307
+ LITDSEKAL +LLSYPLYETL+S+E P+LED +SEVA SLLAAYDSGEL AL +G
Sbjct: 418 GIDLITDSEKALSSLLSYPLYETLASAEGKPILEDGVSEVAKSLLAAYDSGELSGALAEG 477
Query: 306 KPGWQKWVKSFGKSVKRKGKSLFMPLRVLLTGKLHGPDIGASVVLLHSLGTGDIVASHTG 127
+PGWQKW K+FGK +KRKGKSLFMPLRVLLTGKLHGPDIGA+ VLL+ GT V G
Sbjct: 478 QPGWQKWAKNFGKLLKRKGKSLFMPLRVLLTGKLHGPDIGATTVLLYKAGTSGSVVPQAG 537
Query: 126 FVTLEERFKLLREVEWRSFN 67
FVT +ERFK+LREV+W SF+
Sbjct: 538 FVTFDERFKILREVQWESFS 557
>gb|AAY18610.1| glutamyl-tRNA synthetase [Nicotiana benthamiana]
Length = 569
Score = 838 bits (2166), Expect = 0.0
Identities = 414/555 (74%), Positives = 467/555 (84%)
Frame = -1
Query: 1692 SLVHCCNNHHIICLPARSKRCLSIITAATSSVEKEQDDNIVRVRFAPSPTGNLHVGGART 1513
SL++C +H +R S+ +A + + VRVRFAPSPTGNLHVGGART
Sbjct: 23 SLLYCRGDHSYWQSLCSRRRSFSVYASAGNGGD-------VRVRFAPSPTGNLHVGGART 75
Query: 1512 ALFNYLFARSKGGKFVLRIEDTDLERSTKESEEAVLQDLSWLGLHWDEGPDVGGDYGPYR 1333
ALFNYL+AR+KGGKF+LRIEDTDLERSTKESEEAVL+DLSWLGL WDEGP +GG YGPYR
Sbjct: 76 ALFNYLYARAKGGKFILRIEDTDLERSTKESEEAVLRDLSWLGLAWDEGPGIGGGYGPYR 135
Query: 1332 QSERNMLYKQYAEELLNSGHAYRCFCSNEELEKMKEIAKLKQLPPVYTGKWASATNEEVQ 1153
QSERN LYKQ+AE+LL SGH YRCFCSNEELEKMKEIAKLKQLPPVYTG+WASAT EEV
Sbjct: 136 QSERNALYKQFAEKLLQSGHVYRCFCSNEELEKMKEIAKLKQLPPVYTGRWASATEEEVV 195
Query: 1152 EQLRKGTPFTYRFRVPKDGKLKINDLIRGEVSWNLDTLGDFVIMRSNGQPVYNFCVTVDD 973
E+L KGTPFTYRFRVPK+G LKI+DLIRGEVSWNLDTLGDFVIMRSNGQPVYNFCVTVDD
Sbjct: 196 EELAKGTPFTYRFRVPKEGSLKIDDLIRGEVSWNLDTLGDFVIMRSNGQPVYNFCVTVDD 255
Query: 972 ATMAISHVIRAEEHLPNTLRQALIYKALGFRMPYFAHVSLILAPDRSKLSKRHGATSVGQ 793
ATMAISHVIRAEEHLPNTLRQALIYKALGF MP+FAHVSLILAPDRSKLSKRHGATSVGQ
Sbjct: 256 ATMAISHVIRAEEHLPNTLRQALIYKALGFPMPHFAHVSLILAPDRSKLSKRHGATSVGQ 315
Query: 792 FKEMGYLPQAVVNYLALLGWGDGTEDEFYTLEQLVEKFSIERVNKSGAVFDSTKFRWMNG 613
F++MGYLPQA+VNYLALLGWGDGTE+EF+TLEQLVEKF+IERVNKSGA+FDSTK RWMNG
Sbjct: 316 FRDMGYLPQAMVNYLALLGWGDGTENEFFTLEQLVEKFTIERVNKSGAIFDSTKLRWMNG 375
Query: 612 QHXXXXXXXXXXXXLGHHWKSSGILRVSEGLFVEEAVELLKDAVYLITDSEKALLNLLSY 433
QH +G WK +GI S+G+F+++AV LLKD + LITDSEKAL +LLSY
Sbjct: 376 QHLRSLPSEELNRIIGERWKDAGITAESQGIFIQDAVLLLKDGIDLITDSEKALSSLLSY 435
Query: 432 PLYETLSSSEANPVLEDSLSEVADSLLAAYDSGELFNALDQGKPGWQKWVKSFGKSVKRK 253
PLYETL+S+E P+LED +SEVA SLLAAYDSGE AL +G+PGWQKWVK+FGK +KRK
Sbjct: 436 PLYETLASAEGKPILEDGVSEVAKSLLAAYDSGEFSGALAEGQPGWQKWVKNFGKLLKRK 495
Query: 252 GKSLFMPLRVLLTGKLHGPDIGASVVLLHSLGTGDIVASHTGFVTLEERFKLLREVEWRS 73
GKSLFMPLRVLLTGKLHGPDIGA+ VLL+ GT V GFVT +ERFK+LREV+W S
Sbjct: 496 GKSLFMPLRVLLTGKLHGPDIGATTVLLYKAGTSGSVVPQAGFVTFDERFKILREVQWES 555
Query: 72 FNDNQTALESATISH 28
F+ L + ++H
Sbjct: 556 FS-TDVPLSAGAVTH 569