BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00008689
         (1120 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ADZ28515.1| flavonoid-3'-hydroxylase [Camellia nitidissima]        493   e-137
gb|ACV74415.1| putative flavonoid-3'-hydroxylase [Camellia sinen...   482   e-134
dbj|BAE47004.1| flavonoid 3'-hydroxylase [Vitis vinifera]             472   e-131
ref|XP_002284151.1| PREDICTED: flavonoid 3'-monooxygenase [Vitis...   472   e-131
emb|CBI15132.3| unnamed protein product [Vitis vinifera]              471   e-130

>gb|ADZ28515.1| flavonoid-3'-hydroxylase [Camellia nitidissima]
          Length = 518

 Score =  493 bits (1268), Expect = e-137
 Identities = 246/306 (80%), Positives = 272/306 (88%), Gaps = 2/306 (0%)
 Frame = -3

Query: 1118 VVELMTLAGVFNIGDFIPALECLDLQGVAKKMKKLHARFDSFLNNILDEHKI--SGGEKF 945
            VVELM LAGVFNIGDF+PALE LDLQGVA KMKKLHARFDSFLN IL+EHKI  SG    
Sbjct: 213  VVELMVLAGVFNIGDFVPALEWLDLQGVASKMKKLHARFDSFLNKILEEHKINYSGSGAQ 272

Query: 944  MNVDLLSTLLSLKDVEDGEGGKLTDTEIKALLLNLFTAGTDTSSSTVEWAIAEILRNPKI 765
             + DLLSTL+SLKD  DGEGGKLTD EIKALLL+LFTAGTDTSSSTVEWAIAE++R+PKI
Sbjct: 273  KHTDLLSTLISLKDDADGEGGKLTDIEIKALLLDLFTAGTDTSSSTVEWAIAELIRHPKI 332

Query: 764  LAQAQKELDSVIGPDRLVTELDLPQLPYMTAIIKETFRLHPSTPLSLPRMASEDCEINGY 585
            LAQA++ELDS++GPDR VTE DL QL ++ AIIKETFRLHPSTPLSLPRMAS+ CEINGY
Sbjct: 333  LAQAKQELDSIVGPDRRVTESDLAQLTFLQAIIKETFRLHPSTPLSLPRMASDSCEINGY 392

Query: 584  FIPKGSTLLVNVWAIARDENMWADPLVFKPERFLPGGEKPNVDVRGNDFEVIPFGAGRRI 405
            FIPKGSTLLVNVWAIARD + WA+PL F+PERFLPGGEKPNVDVRGNDFEVIPFGAGRRI
Sbjct: 393  FIPKGSTLLVNVWAIARDPDAWAEPLEFRPERFLPGGEKPNVDVRGNDFEVIPFGAGRRI 452

Query: 404  CAGLSLGLRMVQLLTATLVHSFNLQLANGEDVKNINMDEGYGLTLQRAVPLMVHPSPRLA 225
            CAG+SLGLRMVQLLTATLVH+FN  LA+G+  + + MDE YGLTLQRA PLMVHP PRLA
Sbjct: 453  CAGMSLGLRMVQLLTATLVHAFNWDLADGQSAEKLKMDEAYGLTLQRAAPLMVHPRPRLA 512

Query: 224  PQLYEA 207
            P +Y+A
Sbjct: 513  PHVYQA 518


>gb|ACV74415.1| putative flavonoid-3'-hydroxylase [Camellia sinensis]
          Length = 518

 Score =  482 bits (1240), Expect = e-134
 Identities = 243/308 (78%), Positives = 270/308 (87%), Gaps = 4/308 (1%)
 Frame = -3

Query: 1118 VVELMTLAGVFNIGDFIPALECLDLQGVAKKMKKLHARFDSFLNNILDEHKI----SGGE 951
            VVELM LAGVFNIGDF+PALE LD QGVA KMKKLHARFDSFLN IL+EHK+    SG +
Sbjct: 213  VVELMVLAGVFNIGDFVPALEWLDPQGVASKMKKLHARFDSFLNKILEEHKVNYSSSGAQ 272

Query: 950  KFMNVDLLSTLLSLKDVEDGEGGKLTDTEIKALLLNLFTAGTDTSSSTVEWAIAEILRNP 771
            K  + DLLSTL+SLKD  DGEGGKLTD EIKALLL+LFTAGTDTSSSTVEWAIAE++R  
Sbjct: 273  K--HTDLLSTLISLKDDADGEGGKLTDIEIKALLLDLFTAGTDTSSSTVEWAIAELIRLS 330

Query: 770  KILAQAQKELDSVIGPDRLVTELDLPQLPYMTAIIKETFRLHPSTPLSLPRMASEDCEIN 591
            KILAQA++ELDS++GPDR VTE DL QL ++ AIIKETFRLHPSTPLSLPRMAS+ CEIN
Sbjct: 331  KILAQAKQELDSIVGPDRRVTESDLAQLTFLQAIIKETFRLHPSTPLSLPRMASDSCEIN 390

Query: 590  GYFIPKGSTLLVNVWAIARDENMWADPLVFKPERFLPGGEKPNVDVRGNDFEVIPFGAGR 411
            GYFIPKGSTLLVNVWAIARD + WA+PL F+PERFLPGGEKPN DVRGNDFEVIPFGAGR
Sbjct: 391  GYFIPKGSTLLVNVWAIARDPDAWAEPLEFRPERFLPGGEKPNADVRGNDFEVIPFGAGR 450

Query: 410  RICAGLSLGLRMVQLLTATLVHSFNLQLANGEDVKNINMDEGYGLTLQRAVPLMVHPSPR 231
            RICAG+SLGLRMVQLLTATLVH+F+  LA+G+  + +NMDE YGLTLQRA PLMVHP PR
Sbjct: 451  RICAGMSLGLRMVQLLTATLVHAFDWDLADGQSTEKLNMDEAYGLTLQRAAPLMVHPWPR 510

Query: 230  LAPQLYEA 207
            LAP +Y A
Sbjct: 511  LAPHVYRA 518


>dbj|BAE47004.1| flavonoid 3'-hydroxylase [Vitis vinifera]
          Length = 509

 Score =  472 bits (1214), Expect = e-131
 Identities = 232/303 (76%), Positives = 266/303 (87%), Gaps = 1/303 (0%)
 Frame = -3

Query: 1118 VVELMTLAGVFNIGDFIPALECLDLQGVAKKMKKLHARFDSFLNNILDEHKISGGE-KFM 942
            VVELM LAGVFNIGDF+PALE LDLQGVA KMKKLHARFD+FL  I++EHKISG      
Sbjct: 205  VVELMVLAGVFNIGDFVPALEWLDLQGVASKMKKLHARFDAFLGAIVEEHKISGSAGSER 264

Query: 941  NVDLLSTLLSLKDVEDGEGGKLTDTEIKALLLNLFTAGTDTSSSTVEWAIAEILRNPKIL 762
            +VDLLSTL+SLKD  DGEGGKLTD EIKALLLNLFTAGTDTSSSTVEWAIAE++R+P+++
Sbjct: 265  HVDLLSTLISLKDNADGEGGKLTDVEIKALLLNLFTAGTDTSSSTVEWAIAELIRHPEMM 324

Query: 761  AQAQKELDSVIGPDRLVTELDLPQLPYMTAIIKETFRLHPSTPLSLPRMASEDCEINGYF 582
            AQAQ+ELD+V+G  RLVT+LDLP+L Y+ AI+KETFRLHPSTPLSLPRMA+E CEINGY 
Sbjct: 325  AQAQQELDAVVGRGRLVTDLDLPKLTYLQAIVKETFRLHPSTPLSLPRMAAESCEINGYH 384

Query: 581  IPKGSTLLVNVWAIARDENMWADPLVFKPERFLPGGEKPNVDVRGNDFEVIPFGAGRRIC 402
            IPK +TLLVNVWAIARD  +W +PL F+P RFLPGGE+PN DVRGNDFEVIPFGAGRRIC
Sbjct: 385  IPKNATLLVNVWAIARDPEVWEEPLEFRPNRFLPGGERPNADVRGNDFEVIPFGAGRRIC 444

Query: 401  AGLSLGLRMVQLLTATLVHSFNLQLANGEDVKNINMDEGYGLTLQRAVPLMVHPSPRLAP 222
            AG+SLGLRMV LLTATLVH+FN +L  G+  + +NMDE YGLTLQRA PLMVHP PRL+P
Sbjct: 445  AGMSLGLRMVHLLTATLVHAFNWELPEGQVAEKLNMDEAYGLTLQRAAPLMVHPRPRLSP 504

Query: 221  QLY 213
            Q++
Sbjct: 505  QVF 507


>ref|XP_002284151.1| PREDICTED: flavonoid 3'-monooxygenase [Vitis vinifera]
            gi|147801850|emb|CAN75347.1| hypothetical protein
            VITISV_002956 [Vitis vinifera]
          Length = 509

 Score =  472 bits (1214), Expect = e-131
 Identities = 232/303 (76%), Positives = 266/303 (87%), Gaps = 1/303 (0%)
 Frame = -3

Query: 1118 VVELMTLAGVFNIGDFIPALECLDLQGVAKKMKKLHARFDSFLNNILDEHKISGGE-KFM 942
            VVELM LAGVFNIGDF+PALE LDLQGVA KMKKLHARFD+FL  I++EHKISG      
Sbjct: 205  VVELMVLAGVFNIGDFVPALEWLDLQGVASKMKKLHARFDAFLGAIVEEHKISGSAGSER 264

Query: 941  NVDLLSTLLSLKDVEDGEGGKLTDTEIKALLLNLFTAGTDTSSSTVEWAIAEILRNPKIL 762
            +VDLLSTL+SLKD  DGEGGKLTD EIKALLLNLFTAGTDTSSSTVEWAIAE++R+P+++
Sbjct: 265  HVDLLSTLISLKDNADGEGGKLTDVEIKALLLNLFTAGTDTSSSTVEWAIAELIRHPEMM 324

Query: 761  AQAQKELDSVIGPDRLVTELDLPQLPYMTAIIKETFRLHPSTPLSLPRMASEDCEINGYF 582
            AQAQ+ELD+V+G  RLVT+LDLP+L Y+ AI+KETFRLHPSTPLSLPRMA+E CEINGY 
Sbjct: 325  AQAQQELDAVVGRGRLVTDLDLPKLTYLQAIVKETFRLHPSTPLSLPRMAAESCEINGYH 384

Query: 581  IPKGSTLLVNVWAIARDENMWADPLVFKPERFLPGGEKPNVDVRGNDFEVIPFGAGRRIC 402
            IPK +TLLVNVWAIARD  +W +PL F+P RFLPGGE+PN DVRGNDFEVIPFGAGRRIC
Sbjct: 385  IPKNATLLVNVWAIARDPEVWEEPLEFRPNRFLPGGERPNADVRGNDFEVIPFGAGRRIC 444

Query: 401  AGLSLGLRMVQLLTATLVHSFNLQLANGEDVKNINMDEGYGLTLQRAVPLMVHPSPRLAP 222
            AG+SLGLRMV LLTATLVH+FN +L  G+  + +NMDE YGLTLQRA PLMVHP PRL+P
Sbjct: 445  AGMSLGLRMVHLLTATLVHAFNWELPEGQVAEKLNMDEAYGLTLQRAAPLMVHPRPRLSP 504

Query: 221  QLY 213
            Q++
Sbjct: 505  QVF 507


>emb|CBI15132.3| unnamed protein product [Vitis vinifera]
          Length = 451

 Score =  471 bits (1212), Expect = e-130
 Identities = 232/303 (76%), Positives = 266/303 (87%), Gaps = 1/303 (0%)
 Frame = -3

Query: 1118 VVELMTLAGVFNIGDFIPALECLDLQGVAKKMKKLHARFDSFLNNILDEHKISGGE-KFM 942
            VVELM LAGVFNIGDF+PALE LDLQGVA KMKKLHARFD+FL  I++EHKISG      
Sbjct: 84   VVELMVLAGVFNIGDFVPALEWLDLQGVAAKMKKLHARFDAFLGAIVEEHKISGSAGSER 143

Query: 941  NVDLLSTLLSLKDVEDGEGGKLTDTEIKALLLNLFTAGTDTSSSTVEWAIAEILRNPKIL 762
            +VDLLSTL+S++D  DGEGGKLTD EIKALLLNLFTAGTDTSSSTVEWAIAE++R+P+++
Sbjct: 144  HVDLLSTLISVRDNADGEGGKLTDVEIKALLLNLFTAGTDTSSSTVEWAIAELIRHPEMM 203

Query: 761  AQAQKELDSVIGPDRLVTELDLPQLPYMTAIIKETFRLHPSTPLSLPRMASEDCEINGYF 582
            AQAQ+ELD+V+G  RLVT+LDLPQL Y+ AIIKETFRLHPSTPLSLPRMA+E CEINGY 
Sbjct: 204  AQAQQELDAVVGRSRLVTDLDLPQLTYVQAIIKETFRLHPSTPLSLPRMAAESCEINGYH 263

Query: 581  IPKGSTLLVNVWAIARDENMWADPLVFKPERFLPGGEKPNVDVRGNDFEVIPFGAGRRIC 402
            IPK +TLLVNVWAIARD  +W +PL F+P RFLPGGE+PN DVRGNDFEVIPFGAGRRIC
Sbjct: 264  IPKNATLLVNVWAIARDPEVWEEPLEFRPNRFLPGGERPNADVRGNDFEVIPFGAGRRIC 323

Query: 401  AGLSLGLRMVQLLTATLVHSFNLQLANGEDVKNINMDEGYGLTLQRAVPLMVHPSPRLAP 222
            AG+SLGLRMV LLTATLVH+FN +L  G+  + +NMDE YGLTLQRA PLMVHP PRL+P
Sbjct: 324  AGMSLGLRMVHLLTATLVHAFNWELPEGQVAEKLNMDEAYGLTLQRAAPLMVHPRPRLSP 383

Query: 221  QLY 213
            Q++
Sbjct: 384  QVF 386