BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00008122
         (3219 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI19823.3| unnamed protein product [Vitis vinifera]              462   e-127
ref|XP_002278300.1| PREDICTED: cytochrome P450 83B1 [Vitis vinif...   462   e-127
ref|XP_002280472.1| PREDICTED: cytochrome P450 83B1 [Vitis vinif...   462   e-127
ref|XP_002280459.1| PREDICTED: cytochrome P450 83B1 [Vitis vinif...   456   e-125
ref|XP_002280438.1| PREDICTED: cytochrome P450 83B1 [Vitis vinif...   450   e-123

>emb|CBI19823.3| unnamed protein product [Vitis vinifera]
          Length = 912

 Score =  462 bits (1189), Expect = e-127
 Identities = 227/409 (55%), Positives = 300/409 (73%), Gaps = 2/409 (0%)
 Frame = +1

Query: 394  KSSSQLPTPPGPRGLPFIGNLHQLDISNFSNHLWQLSKKYGPIMHLKLGYLSAIVISSPK 573
            K+   L  PPGP GLPFIGNLHQ+D S    +LWQLSK+YG +M L+LG++  IV+SS +
Sbjct: 438  KNGGLLHLPPGPPGLPFIGNLHQMDNSARHRYLWQLSKQYGSLMSLRLGFIPTIVVSSAR 497

Query: 574  MAKEVLKTHDLSFCSRPTLFGLKKMSYNCCDVVLSPYSEYWREMRKIVTLHLFSNSRVHS 753
            +AKEV+KTHDL F SRP+L G +++SYNC D+  SPY++YWREMRKI  LHLF+  RV S
Sbjct: 498  IAKEVMKTHDLEFASRPSLIGPQRLSYNCLDLAFSPYNDYWREMRKICVLHLFTLKRVQS 557

Query: 754  FRDVREDEVFQMIRGISKQASSSEVTNLTSIIMPLTSTIICRVAFGKKFDE--VYMKRFE 927
            +  +RE EV QMI  ISK AS+S++ NL+  +M LTSTIICRVAFGK++++      RF 
Sbjct: 558  YTPIREYEVSQMIEKISKLASASKLINLSETLMFLTSTIICRVAFGKRYEDEGFERSRFH 617

Query: 928  GMLHECQLVMANFYFSDNFPLLGWLDKVIGSTAKLDKIFRDMDLFYQELIDEHTSPTRPS 1107
            G+L++ Q ++ +F+FSD+FPL+GWLDK+ G TA+L+K FRDMDLFYQE+I++H  P R  
Sbjct: 618  GLLNDAQAMLGSFFFSDHFPLIGWLDKLTGLTARLEKTFRDMDLFYQEIIEDHLKPDRKK 677

Query: 1108 SMDGDVIDILLQLKDDAQPSSFKITLDNIKAILMNIFVAGXXXXXXXXXXXXXSLVKNPK 1287
                D+ D+L+ L+ D    +  IT D+IK +LMNIFV G             +L+KNP+
Sbjct: 678  QEQEDITDVLIGLQKD-NSFAIDITWDHIKGVLMNIFVGGTDTGAATVIWAMTALMKNPR 736

Query: 1288 AMKKAQDEVRNLMKGKDRINEDDLQKIELPYLEAVIKESLRLYPIVPLLIPRETIDDCVI 1467
             MKKAQ+EVRN    K  I EDD++K  LPYL+AV+KE++RL P VPLLIPRET+  C I
Sbjct: 737  VMKKAQEEVRNTFGKKGFIGEDDVEK--LPYLKAVVKETMRLLPAVPLLIPRETLQKCSI 794

Query: 1468 DGYQIKAKTIVYVNALAIGRDPQVWENAEEFNPERFLDSKIDFKGQDFE 1614
            DGY+I  KT+V+VNA AIGRDP+ WEN EEF PERFL S +DF+GQ+++
Sbjct: 795  DGYEIPPKTLVFVNAWAIGRDPEAWENPEEFIPERFLGSSVDFRGQNYK 843



 Score =  399 bits (1024), Expect = e-108
 Identities = 196/370 (52%), Positives = 265/370 (71%), Gaps = 2/370 (0%)
 Frame = +1

Query: 394  KSSSQLPTPPGPRGLPFIGNLHQLDISNFSNHLWQLSKKYGPIMHLKLGYLSAIVISSPK 573
            K+   L  PPGP GLP IGNLHQ+D S    +LWQLSK+YGP+M L+LG++  IV+SS +
Sbjct: 24   KNGGLLHLPPGPPGLPVIGNLHQMDNSAPHRYLWQLSKQYGPLMSLRLGFIPTIVVSSAR 83

Query: 574  MAKEVLKTHDLSFCSRPTLFGLKKMSYNCCDVVLSPYSEYWREMRKIVTLHLFSNSRVHS 753
            +AKEV+KTHDL F SRP+L G +++SYNC D+  SPY++YWREMRKI  LHLF+  RV S
Sbjct: 84   IAKEVMKTHDLKFASRPSLIGPRRLSYNCLDLAFSPYNDYWREMRKICVLHLFTLKRVQS 143

Query: 754  FRDVREDEVFQMIRGISKQASSSEVTNLTSIIMPLTSTIICRVAFGKKFDE--VYMKRFE 927
            +  +RE EV QMI  ISK AS+S++ NL+  +M LT TIICRV+FGK++++      RF 
Sbjct: 144  YTPIREYEVSQMIEKISKLASASKLINLSETVMFLTITIICRVSFGKRYEDEGCETSRFH 203

Query: 928  GMLHECQLVMANFYFSDNFPLLGWLDKVIGSTAKLDKIFRDMDLFYQELIDEHTSPTRPS 1107
            G+L++ Q ++ +F+FSD+FPL+GWLDK+ G TA+L+K  RDMDLFYQE+I++H  P R  
Sbjct: 204  GLLNDAQAMLGSFFFSDHFPLMGWLDKLTGLTARLEKTLRDMDLFYQEIIEDHLKPDRKK 263

Query: 1108 SMDGDVIDILLQLKDDAQPSSFKITLDNIKAILMNIFVAGXXXXXXXXXXXXXSLVKNPK 1287
                D+ D+L++L+ D    +  IT D+IK +LMNIFV G             +L+KNP+
Sbjct: 264  QEQEDITDVLIELQKD-NSFAIDITWDHIKGVLMNIFVGGTDAGTATVIWAMTALMKNPR 322

Query: 1288 AMKKAQDEVRNLMKGKDRINEDDLQKIELPYLEAVIKESLRLYPIVPLLIPRETIDDCVI 1467
             MKKAQ+EVRN    K  I EDD++K  LPYL+AV+KE++RL P  PLL+PRET+  C I
Sbjct: 323  VMKKAQEEVRNTFGKKGFIGEDDVEK--LPYLKAVVKETMRLLPAAPLLLPRETLQKCSI 380

Query: 1468 DGYQIKAKTI 1497
            DGY+I  KT+
Sbjct: 381  DGYEIPPKTL 390



 Score = 75.1 bits (183), Expect = 1e-10
 Identities = 31/43 (72%), Positives = 38/43 (88%)
 Frame = +2

Query: 1754 NLLYSFDWELPAGIEKEDIDTDVLPGISMHKKNPLCLVPKNYH 1882
            NLLYSFDWE+PAG+ KEDID DV+PG++MHKKN LCL+ K Y+
Sbjct: 870  NLLYSFDWEMPAGMNKEDIDFDVIPGLTMHKKNALCLMAKKYN 912


>ref|XP_002278300.1| PREDICTED: cytochrome P450 83B1 [Vitis vinifera]
          Length = 495

 Score =  462 bits (1189), Expect = e-127
 Identities = 227/409 (55%), Positives = 304/409 (74%), Gaps = 2/409 (0%)
 Frame = +1

Query: 394  KSSSQLPTPPGPRGLPFIGNLHQLDISNFSNHLWQLSKKYGPIMHLKLGYLSAIVISSPK 573
            ++  + P PPGP GLP IGNL QLD S    +LW+LSK+YGP+M L+LG++  +V+SS +
Sbjct: 20   RNRRRTPLPPGPPGLPLIGNLLQLDKSAPHIYLWRLSKQYGPLMILRLGFVPTLVVSSAR 79

Query: 574  MAKEVLKTHDLSFCSRPTLFGLKKMSYNCCDVVLSPYSEYWREMRKIVTLHLFSNSRVHS 753
            MAKEV+KTHDL F  RP+L GL+K+SYN  DV  SPY++YWREMRKI  LHLF++ R  S
Sbjct: 80   MAKEVMKTHDLEFSGRPSLLGLRKLSYNGLDVAFSPYNDYWREMRKICVLHLFNSKRAQS 139

Query: 754  FRDVREDEVFQMIRGISKQASSSEVTNLTSIIMPLTSTIICRVAFGKKFDE--VYMKRFE 927
            FR +REDEV +MI+ IS+ AS+S++TNL+ I++ LTSTIICRVAF K++D+      RF+
Sbjct: 140  FRPIREDEVLEMIKKISQFASASKLTNLSEILISLTSTIICRVAFSKRYDDEGYERSRFQ 199

Query: 928  GMLHECQLVMANFYFSDNFPLLGWLDKVIGSTAKLDKIFRDMDLFYQELIDEHTSPTRPS 1107
             ++ E Q V+  FYFSD FPL+GW+DK+ G  A  DK F++ DLFYQE+IDEH  P RP 
Sbjct: 200  KLVGEGQAVVGGFYFSDYFPLMGWVDKLTGMIALADKNFKEFDLFYQEIIDEHLDPNRPE 259

Query: 1108 SMDGDVIDILLQLKDDAQPSSFKITLDNIKAILMNIFVAGXXXXXXXXXXXXXSLVKNPK 1287
                D+ D+LL+L+ + +  +  +T D+IKA+LMNIF+AG              L+KNP+
Sbjct: 260  PEKEDITDVLLKLQKN-RLFTIDLTFDHIKAVLMNIFLAGTDTSAATLVWAMTMLMKNPR 318

Query: 1288 AMKKAQDEVRNLMKGKDRINEDDLQKIELPYLEAVIKESLRLYPIVPLLIPRETIDDCVI 1467
             M KAQ+E+RNL+  K  ++EDDLQK  LPYL+A++KE++RL+P  PLL+PRET++ CVI
Sbjct: 319  TMTKAQEELRNLIGKKGFVDEDDLQK--LPYLKAIVKETMRLHPASPLLVPRETLEKCVI 376

Query: 1468 DGYQIKAKTIVYVNALAIGRDPQVWENAEEFNPERFLDSKIDFKGQDFE 1614
            DGY+I  KT+VYVNA AIGRDP+ WEN EEF PERFL + IDFKGQD++
Sbjct: 377  DGYEIPPKTLVYVNAWAIGRDPESWENPEEFMPERFLGTSIDFKGQDYQ 425



 Score = 70.5 bits (171), Expect = 3e-09
 Identities = 30/40 (75%), Positives = 35/40 (87%)
 Frame = +2

Query: 1754 NLLYSFDWELPAGIEKEDIDTDVLPGISMHKKNPLCLVPK 1873
            NLLYSFDWE+PAG+ KEDID DV PGI+MHKKN LCL+ +
Sbjct: 452  NLLYSFDWEMPAGMNKEDIDIDVKPGITMHKKNALCLLAR 491


>ref|XP_002280472.1| PREDICTED: cytochrome P450 83B1 [Vitis vinifera]
            gi|147832399|emb|CAN64422.1| hypothetical protein
            VITISV_032274 [Vitis vinifera]
          Length = 498

 Score =  462 bits (1189), Expect = e-127
 Identities = 227/409 (55%), Positives = 300/409 (73%), Gaps = 2/409 (0%)
 Frame = +1

Query: 394  KSSSQLPTPPGPRGLPFIGNLHQLDISNFSNHLWQLSKKYGPIMHLKLGYLSAIVISSPK 573
            K+   L  PPGP GLPFIGNLHQ+D S    +LWQLSK+YG +M L+LG++  IV+SS +
Sbjct: 24   KNGGLLHLPPGPPGLPFIGNLHQMDNSARHRYLWQLSKQYGSLMSLRLGFIPTIVVSSAR 83

Query: 574  MAKEVLKTHDLSFCSRPTLFGLKKMSYNCCDVVLSPYSEYWREMRKIVTLHLFSNSRVHS 753
            +AKEV+KTHDL F SRP+L G +++SYNC D+  SPY++YWREMRKI  LHLF+  RV S
Sbjct: 84   IAKEVMKTHDLEFASRPSLIGPQRLSYNCLDLAFSPYNDYWREMRKICVLHLFTLKRVQS 143

Query: 754  FRDVREDEVFQMIRGISKQASSSEVTNLTSIIMPLTSTIICRVAFGKKFDE--VYMKRFE 927
            +  +RE EV QMI  ISK AS+S++ NL+  +M LTSTIICRVAFGK++++      RF 
Sbjct: 144  YTPIREYEVSQMIEKISKLASASKLINLSETLMFLTSTIICRVAFGKRYEDEGFERSRFH 203

Query: 928  GMLHECQLVMANFYFSDNFPLLGWLDKVIGSTAKLDKIFRDMDLFYQELIDEHTSPTRPS 1107
            G+L++ Q ++ +F+FSD+FPL+GWLDK+ G TA+L+K FRDMDLFYQE+I++H  P R  
Sbjct: 204  GLLNDAQAMLGSFFFSDHFPLIGWLDKLTGLTARLEKTFRDMDLFYQEIIEDHLKPDRKK 263

Query: 1108 SMDGDVIDILLQLKDDAQPSSFKITLDNIKAILMNIFVAGXXXXXXXXXXXXXSLVKNPK 1287
                D+ D+L+ L+ D    +  IT D+IK +LMNIFV G             +L+KNP+
Sbjct: 264  QEQEDITDVLIGLQKD-NSFAIDITWDHIKGVLMNIFVGGTDTGAATVIWAMTALMKNPR 322

Query: 1288 AMKKAQDEVRNLMKGKDRINEDDLQKIELPYLEAVIKESLRLYPIVPLLIPRETIDDCVI 1467
             MKKAQ+EVRN    K  I EDD++K  LPYL+AV+KE++RL P VPLLIPRET+  C I
Sbjct: 323  VMKKAQEEVRNTFGKKGFIGEDDVEK--LPYLKAVVKETMRLLPAVPLLIPRETLQKCSI 380

Query: 1468 DGYQIKAKTIVYVNALAIGRDPQVWENAEEFNPERFLDSKIDFKGQDFE 1614
            DGY+I  KT+V+VNA AIGRDP+ WEN EEF PERFL S +DF+GQ+++
Sbjct: 381  DGYEIPPKTLVFVNAWAIGRDPEAWENPEEFIPERFLGSSVDFRGQNYK 429



 Score = 75.1 bits (183), Expect = 1e-10
 Identities = 31/43 (72%), Positives = 38/43 (88%)
 Frame = +2

Query: 1754 NLLYSFDWELPAGIEKEDIDTDVLPGISMHKKNPLCLVPKNYH 1882
            NLLYSFDWE+PAG+ KEDID DV+PG++MHKKN LCL+ K Y+
Sbjct: 456  NLLYSFDWEMPAGMNKEDIDFDVIPGLTMHKKNALCLMAKKYN 498


>ref|XP_002280459.1| PREDICTED: cytochrome P450 83B1 [Vitis vinifera]
          Length = 498

 Score =  456 bits (1174), Expect = e-125
 Identities = 222/409 (54%), Positives = 298/409 (72%), Gaps = 2/409 (0%)
 Frame = +1

Query: 394  KSSSQLPTPPGPRGLPFIGNLHQLDISNFSNHLWQLSKKYGPIMHLKLGYLSAIVISSPK 573
            K+   L  PPGP GLP IGNLHQ+D S    +LWQLSK+YGP+M L+LG++  IV+SS +
Sbjct: 24   KNGGLLHLPPGPPGLPVIGNLHQMDNSAPHRYLWQLSKQYGPLMSLRLGFIPTIVVSSAR 83

Query: 574  MAKEVLKTHDLSFCSRPTLFGLKKMSYNCCDVVLSPYSEYWREMRKIVTLHLFSNSRVHS 753
            +AKEV+KTHDL F SRP+L G +++SYNC D+  SPY++YWREMRKI  LHLF+  RV S
Sbjct: 84   IAKEVMKTHDLKFASRPSLIGPRRLSYNCLDLAFSPYNDYWREMRKICVLHLFTLKRVQS 143

Query: 754  FRDVREDEVFQMIRGISKQASSSEVTNLTSIIMPLTSTIICRVAFGKKFDE--VYMKRFE 927
            +  +RE EV QMI  ISK AS+S++ NL+  +M LT TIICRV+FGK++++      RF 
Sbjct: 144  YTPIREYEVSQMIEKISKLASASKLINLSETVMFLTITIICRVSFGKRYEDEGCETSRFH 203

Query: 928  GMLHECQLVMANFYFSDNFPLLGWLDKVIGSTAKLDKIFRDMDLFYQELIDEHTSPTRPS 1107
            G+L++ Q ++ +F+FSD+FPL+GWLDK+ G TA+L+K  RDMDLFYQE+I++H  P R  
Sbjct: 204  GLLNDAQAMLGSFFFSDHFPLMGWLDKLTGLTARLEKTLRDMDLFYQEIIEDHLKPDRKK 263

Query: 1108 SMDGDVIDILLQLKDDAQPSSFKITLDNIKAILMNIFVAGXXXXXXXXXXXXXSLVKNPK 1287
                D+ D+L++L+ D    +  IT D+IK +LMNIFV G             +L+KNP+
Sbjct: 264  QEQEDITDVLIELQKD-NSFAIDITWDHIKGVLMNIFVGGTDAGTATVIWAMTALMKNPR 322

Query: 1288 AMKKAQDEVRNLMKGKDRINEDDLQKIELPYLEAVIKESLRLYPIVPLLIPRETIDDCVI 1467
             MKKAQ+EVRN    K  I EDD++K  LPYL+AV+KE++RL P  PLL+PRET+  C I
Sbjct: 323  VMKKAQEEVRNTFGKKGFIGEDDVEK--LPYLKAVVKETMRLLPAAPLLLPRETLQKCSI 380

Query: 1468 DGYQIKAKTIVYVNALAIGRDPQVWENAEEFNPERFLDSKIDFKGQDFE 1614
            DGY+I  KT+V+VNA AIGRDP+ WEN EEF PERFL S +DF+GQ+++
Sbjct: 381  DGYEIPPKTLVFVNAWAIGRDPEAWENPEEFIPERFLGSSVDFRGQNYK 429



 Score = 75.1 bits (183), Expect = 1e-10
 Identities = 31/43 (72%), Positives = 38/43 (88%)
 Frame = +2

Query: 1754 NLLYSFDWELPAGIEKEDIDTDVLPGISMHKKNPLCLVPKNYH 1882
            NLLYSFDWE+PAG+ KEDID DV+PG++MHKKN LCL+ K Y+
Sbjct: 456  NLLYSFDWEMPAGMNKEDIDFDVIPGLTMHKKNALCLMAKKYN 498


>ref|XP_002280438.1| PREDICTED: cytochrome P450 83B1 [Vitis vinifera]
          Length = 496

 Score =  450 bits (1158), Expect = e-123
 Identities = 222/401 (55%), Positives = 294/401 (73%), Gaps = 2/401 (0%)
 Frame = +1

Query: 418  PPGPRGLPFIGNLHQLDISNFSNHLWQLSKKYGPIMHLKLGYLSAIVISSPKMAKEVLKT 597
            PPGP GLPFIGNLHQ+D S    +LWQLSK+YGP+M L+LG++  IV+SS K+AKEV+KT
Sbjct: 30   PPGPPGLPFIGNLHQMDNSAPHRYLWQLSKQYGPLMSLRLGFVPTIVVSSAKIAKEVMKT 89

Query: 598  HDLSFCSRPTLFGLKKMSYNCCDVVLSPYSEYWREMRKIVTLHLFSNSRVHSFRDVREDE 777
             DL F SRP+L G +++SYN  D+  SPY++YWREMRKI  LHLF+  RV S+  +RE E
Sbjct: 90   QDLEFASRPSLIGQQRLSYNGLDLAFSPYNDYWREMRKICVLHLFTLKRVKSYTSIREYE 149

Query: 778  VFQMIRGISKQASSSEVTNLTSIIMPLTSTIICRVAFGKKF--DEVYMKRFEGMLHECQL 951
            V QMI  ISK AS+S++ NL+  +M LTSTIICRVAFGK++  +     RF G+L++ Q 
Sbjct: 150  VSQMIEKISKLASASKLINLSEALMFLTSTIICRVAFGKRYEGEGCERSRFHGLLNDAQA 209

Query: 952  VMANFYFSDNFPLLGWLDKVIGSTAKLDKIFRDMDLFYQELIDEHTSPTRPSSMDGDVID 1131
            ++ +F+FSD+FPL+GWLDK+ G TA+L+K FR+MDLFYQE+I+EH  P R      D+ D
Sbjct: 210  MLGSFFFSDHFPLMGWLDKLTGLTARLEKTFREMDLFYQEIIEEHLKPDRKKQELEDITD 269

Query: 1132 ILLQLKDDAQPSSFKITLDNIKAILMNIFVAGXXXXXXXXXXXXXSLVKNPKAMKKAQDE 1311
            +L+ L+ D    +  IT D+IK +LMNIF+ G             +L+KNP+ MKKAQ+E
Sbjct: 270  VLIGLRKD-NDFAIDITWDHIKGVLMNIFLGGTDTGAATVTWAMTALMKNPRVMKKAQEE 328

Query: 1312 VRNLMKGKDRINEDDLQKIELPYLEAVIKESLRLYPIVPLLIPRETIDDCVIDGYQIKAK 1491
            VRN    K  I EDD++K  LPYL+AV+KE++RL P VPLL+PRET+  C +DGY+I  K
Sbjct: 329  VRNTFGKKGFIGEDDVEK--LPYLKAVVKETMRLLPSVPLLVPRETLQKCSLDGYEIPPK 386

Query: 1492 TIVYVNALAIGRDPQVWENAEEFNPERFLDSKIDFKGQDFE 1614
            T+V+VNA AIGRDP+ WEN EEF PERFL S +DF+GQ ++
Sbjct: 387  TLVFVNAWAIGRDPEAWENPEEFMPERFLGSSVDFRGQHYK 427



 Score = 72.4 bits (176), Expect = 7e-10
 Identities = 30/43 (69%), Positives = 37/43 (86%)
 Frame = +2

Query: 1754 NLLYSFDWELPAGIEKEDIDTDVLPGISMHKKNPLCLVPKNYH 1882
            NLL+SFDWE+PAG+ +EDID D +PGI+MHKKN LCLV K Y+
Sbjct: 454  NLLHSFDWEMPAGMNEEDIDLDTIPGIAMHKKNALCLVAKKYN 496