BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00006824
         (2721 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002268471.2| PREDICTED: LOW QUALITY PROTEIN: villin-4 [Vi...  1031   0.0  
emb|CBI17857.3| unnamed protein product [Vitis vinifera]             1031   0.0  
ref|XP_002329670.1| predicted protein [Populus trichocarpa] gi|2...   986   0.0  
ref|XP_002324461.1| predicted protein [Populus trichocarpa] gi|2...   977   0.0  
ref|XP_003533726.1| PREDICTED: villin-4-like [Glycine max]            967   0.0  

>ref|XP_002268471.2| PREDICTED: LOW QUALITY PROTEIN: villin-4 [Vitis vinifera]
          Length = 1002

 Score = 1031 bits (2667), Expect(2) = 0.0
 Identities = 509/643 (79%), Positives = 565/643 (87%), Gaps = 1/643 (0%)
 Frame = +1

Query: 1    IFQFNGSNSSIQERAKALEVVQYIKDTYHDGKCEIAAIEDGKLMADADTGEFWGLFGGFA 180
            IFQFNGSNSSIQERAKALEVVQYIKDTYHDGKCE+A+IEDGKLMADA+TGEFWG FGGFA
Sbjct: 217  IFQFNGSNSSIQERAKALEVVQYIKDTYHDGKCEVASIEDGKLMADAETGEFWGFFGGFA 276

Query: 181  PLPRKTVTEDANNTGDISTRLFSVVKGKHEPVDVDSLKRELLDTYKCYLLDCGSEVFVWM 360
            PLPRKT  ED      +  +LF ++KG+ EPV  DSL RELLDT KCY+LDCG EVFVWM
Sbjct: 277  PLPRKTANEDDKAVDSLPAKLFCILKGQAEPVQADSLTRELLDTNKCYILDCGVEVFVWM 336

Query: 361  GRNTSLDERKSASEAAEELLRTIPRPKTHIIRVMEGFETVMFRSKFDSWPQSTDVAVSED 540
            GRNTSLDERKSAS AAEELLR++ RPK+HIIRV+EGFETVMFRSKFD WP++T V VSED
Sbjct: 337  GRNTSLDERKSASSAAEELLRSLDRPKSHIIRVIEGFETVMFRSKFDMWPETTAVTVSED 396

Query: 541  GRGKVAALLKRQGVNVKGLLKATPAKEEPQPYIDCTGNLQVWRVDVEGKTPLTSSDQSKF 720
            GRGKVAALLKRQGVNVKGLLKA P KEEPQPYIDCTGNLQVWRV+ + KT L++SDQSKF
Sbjct: 397  GRGKVAALLKRQGVNVKGLLKAAPVKEEPQPYIDCTGNLQVWRVNGQEKTLLSASDQSKF 456

Query: 721  FCGDCYIFQYSYPGELGEEYLVGTWFGEESVEEDRNSAISQASKMVESLKFLPVQARICE 900
            + GDCYIFQYSYPGE  EE+L+GTWFG++SVEE+R SAIS A+KMVESLKFLP QARI E
Sbjct: 457  YSGDCYIFQYSYPGEDKEEHLIGTWFGKQSVEEERTSAISLATKMVESLKFLPAQARIYE 516

Query: 901  GNEXXXXXXXXXXXLVFKGGVSDRYKKYIADKELRDVTYSEDGLALFRVQGTGPENMQAI 1080
            GNE           +VFKGGVSD YKKYIA+KE+ D TY+ED +ALFRVQG+GP+NMQAI
Sbjct: 517  GNEPIQFFSIFQSFIVFKGGVSDGYKKYIAEKEVPDDTYTEDRVALFRVQGSGPDNMQAI 576

Query: 1081 QVEAVASSLNSSYCYILHNGSSVFTWLGNLTTSEDQELVERLLDLIKPNMQSRPQKEGSE 1260
            QVE VASSLNSSYCYIL++GSSVF W GNLTT EDQELVER LD+IKPN+QS+PQKEGSE
Sbjct: 577  QVEPVASSLNSSYCYILNSGSSVFNWSGNLTTPEDQELVERQLDVIKPNVQSKPQKEGSE 636

Query: 1261 SEQFWELLGGKSEYPSQKVGRDVESDPHLFSCSFS-ADLKVTEIHNFDQDDLMTEDIFIL 1437
            SEQFWE LGGKSEYPSQK+ RD E+DPHLFSC+FS  +LKVTEI NF QDDLMTEDIFIL
Sbjct: 637  SEQFWEFLGGKSEYPSQKIARDAENDPHLFSCTFSKGNLKVTEIFNFTQDDLMTEDIFIL 696

Query: 1438 DCHSEIFVWVGQQVNTKYRKDALTIGEKFLARDFLLEKISSQAPIYIVMEGSEPTFFTRF 1617
            DCHSEIFVWVGQQV++K R  ALTIGEKFL RDFLLEK+S  APIYI+MEGSEP FFTRF
Sbjct: 697  DCHSEIFVWVGQQVDSKNRMHALTIGEKFLERDFLLEKLSHTAPIYIIMEGSEPPFFTRF 756

Query: 1618 FTWDSTKSAMHGNSFQRKLAMVKNGGTPVLDKPKRRPAVSYGGRSAALPEKSQRARSVSF 1797
            FTWDS KSAM GNSFQRKLA+VKNG +P  +KPKRR  VSYGGRS++LPEKSQR+RS+SF
Sbjct: 757  FTWDSGKSAMQGNSFQRKLAIVKNGISPTPEKPKRRTPVSYGGRSSSLPEKSQRSRSMSF 816

Query: 1798 SPERVRVRGRSPAFNALAAAFENPNGRNLSTPPPVVKKLYPKS 1926
            SP+RVRVRGRSPAFNALAA FENPN RNLSTPPP+V+KLYPKS
Sbjct: 817  SPDRVRVRGRSPAFNALAANFENPNSRNLSTPPPMVRKLYPKS 859



 Score =  160 bits (406), Expect(2) = 0.0
 Identities = 79/112 (70%), Positives = 95/112 (84%), Gaps = 2/112 (1%)
 Frame = +3

Query: 2022 ESLIPRSVKVSPKAPITKP--DTESKENAMSSRIEALAIQEDVKEGEADDEEGLVTYPYE 2195
            E ++P++ KV+ +AP  KP  +T SKE AMSSRIEAL I+EDVKEGEA+DEEGL  YPYE
Sbjct: 887  EPVVPKTPKVTEEAPKPKPKPETNSKEKAMSSRIEALTIEEDVKEGEAEDEEGLPIYPYE 946

Query: 2196 RLTTLSADPVTDIDVTKRETYLSSQEFKEKFGMTRAAFYKLPKWKQNKLKMS 2351
            RL T S +PV +IDVTKRETYLSS+EF++KFGMT+ AFYKLPKWKQNKLKM+
Sbjct: 947  RLKTTSIEPVAEIDVTKRETYLSSEEFRQKFGMTKDAFYKLPKWKQNKLKMA 998



 Score = 90.5 bits (223), Expect = 2e-15
 Identities = 86/375 (22%), Positives = 162/375 (43%), Gaps = 17/375 (4%)
 Frame = +1

Query: 655  LQVWRVDVEGKTPLTSSDQSKFFCGDCYIFQYSYPGELGE-EYLVGTWFGEESVEEDRNS 831
            +++WR++     P+  S   KFF GD Y+   +   + G   + +  W G+++ +++  +
Sbjct: 62   IEIWRIENFRPMPVPKSSYGKFFTGDSYVILKTTALKNGALRHDIHYWLGKDTTQDEAGT 121

Query: 832  AISQASKMVESLKFLPVQARICEGNEXXXXXXXXXXXLVFK-GGVSDRYKKYIADKELRD 1008
            A  +  ++  +L    VQ R  +G+E           ++ + GGV+  +K   A+ E + 
Sbjct: 122  AAVKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIPQPGGVASGFKHAEAE-EHKT 180

Query: 1009 VTYSEDGLALFRVQGTGPENMQAIQVEAVASSLNSSYCYILHNGSSVFTWLGNLTTSEDQ 1188
              Y   G  +  V+          +V    SSLN    +IL   S +F + G+ ++ +++
Sbjct: 181  RLYVCKGKHVVHVK----------EVSFARSSLNHDDIFILDTKSKIFQFNGSNSSIQER 230

Query: 1189 ELVERLLDLIK----------PNMQSRPQKEGSESEQFWELLGGKSEYPSQKVGRD---V 1329
                 ++  IK           +++       +E+ +FW   GG +  P +    D   V
Sbjct: 231  AKALEVVQYIKDTYHDGKCEVASIEDGKLMADAETGEFWGFFGGFAPLPRKTANEDDKAV 290

Query: 1330 ESDPHLFSCSFSADLKVTEIHNFDQDDLMTEDIFILDCHSEIFVWVGQQVNTKYRKDALT 1509
            +S P    C      +  +  +  ++ L T   +ILDC  E+FVW+G+  +   RK A +
Sbjct: 291  DSLPAKLFCILKGQAEPVQADSLTRELLDTNKCYILDCGVEVFVWMGRNTSLDERKSASS 350

Query: 1510 IGEKFLARDFLLEKISSQAPIYIVMEGSEPTFF-TRFFTWDSTKSAMHGNSFQRKL-AMV 1683
              E+ L     L++  S   I  V+EG E   F ++F  W  T +       + K+ A++
Sbjct: 351  AAEELLRS---LDRPKSH--IIRVIEGFETVMFRSKFDMWPETTAVTVSEDGRGKVAALL 405

Query: 1684 KNGGTPVLDKPKRRP 1728
            K  G  V    K  P
Sbjct: 406  KRQGVNVKGLLKAAP 420


>emb|CBI17857.3| unnamed protein product [Vitis vinifera]
          Length = 961

 Score = 1031 bits (2667), Expect(2) = 0.0
 Identities = 509/643 (79%), Positives = 565/643 (87%), Gaps = 1/643 (0%)
 Frame = +1

Query: 1    IFQFNGSNSSIQERAKALEVVQYIKDTYHDGKCEIAAIEDGKLMADADTGEFWGLFGGFA 180
            IFQFNGSNSSIQERAKALEVVQYIKDTYHDGKCE+A+IEDGKLMADA+TGEFWG FGGFA
Sbjct: 176  IFQFNGSNSSIQERAKALEVVQYIKDTYHDGKCEVASIEDGKLMADAETGEFWGFFGGFA 235

Query: 181  PLPRKTVTEDANNTGDISTRLFSVVKGKHEPVDVDSLKRELLDTYKCYLLDCGSEVFVWM 360
            PLPRKT  ED      +  +LF ++KG+ EPV  DSL RELLDT KCY+LDCG EVFVWM
Sbjct: 236  PLPRKTANEDDKAVDSLPAKLFCILKGQAEPVQADSLTRELLDTNKCYILDCGVEVFVWM 295

Query: 361  GRNTSLDERKSASEAAEELLRTIPRPKTHIIRVMEGFETVMFRSKFDSWPQSTDVAVSED 540
            GRNTSLDERKSAS AAEELLR++ RPK+HIIRV+EGFETVMFRSKFD WP++T V VSED
Sbjct: 296  GRNTSLDERKSASSAAEELLRSLDRPKSHIIRVIEGFETVMFRSKFDMWPETTAVTVSED 355

Query: 541  GRGKVAALLKRQGVNVKGLLKATPAKEEPQPYIDCTGNLQVWRVDVEGKTPLTSSDQSKF 720
            GRGKVAALLKRQGVNVKGLLKA P KEEPQPYIDCTGNLQVWRV+ + KT L++SDQSKF
Sbjct: 356  GRGKVAALLKRQGVNVKGLLKAAPVKEEPQPYIDCTGNLQVWRVNGQEKTLLSASDQSKF 415

Query: 721  FCGDCYIFQYSYPGELGEEYLVGTWFGEESVEEDRNSAISQASKMVESLKFLPVQARICE 900
            + GDCYIFQYSYPGE  EE+L+GTWFG++SVEE+R SAIS A+KMVESLKFLP QARI E
Sbjct: 416  YSGDCYIFQYSYPGEDKEEHLIGTWFGKQSVEEERTSAISLATKMVESLKFLPAQARIYE 475

Query: 901  GNEXXXXXXXXXXXLVFKGGVSDRYKKYIADKELRDVTYSEDGLALFRVQGTGPENMQAI 1080
            GNE           +VFKGGVSD YKKYIA+KE+ D TY+ED +ALFRVQG+GP+NMQAI
Sbjct: 476  GNEPIQFFSIFQSFIVFKGGVSDGYKKYIAEKEVPDDTYTEDRVALFRVQGSGPDNMQAI 535

Query: 1081 QVEAVASSLNSSYCYILHNGSSVFTWLGNLTTSEDQELVERLLDLIKPNMQSRPQKEGSE 1260
            QVE VASSLNSSYCYIL++GSSVF W GNLTT EDQELVER LD+IKPN+QS+PQKEGSE
Sbjct: 536  QVEPVASSLNSSYCYILNSGSSVFNWSGNLTTPEDQELVERQLDVIKPNVQSKPQKEGSE 595

Query: 1261 SEQFWELLGGKSEYPSQKVGRDVESDPHLFSCSFS-ADLKVTEIHNFDQDDLMTEDIFIL 1437
            SEQFWE LGGKSEYPSQK+ RD E+DPHLFSC+FS  +LKVTEI NF QDDLMTEDIFIL
Sbjct: 596  SEQFWEFLGGKSEYPSQKIARDAENDPHLFSCTFSKGNLKVTEIFNFTQDDLMTEDIFIL 655

Query: 1438 DCHSEIFVWVGQQVNTKYRKDALTIGEKFLARDFLLEKISSQAPIYIVMEGSEPTFFTRF 1617
            DCHSEIFVWVGQQV++K R  ALTIGEKFL RDFLLEK+S  APIYI+MEGSEP FFTRF
Sbjct: 656  DCHSEIFVWVGQQVDSKNRMHALTIGEKFLERDFLLEKLSHTAPIYIIMEGSEPPFFTRF 715

Query: 1618 FTWDSTKSAMHGNSFQRKLAMVKNGGTPVLDKPKRRPAVSYGGRSAALPEKSQRARSVSF 1797
            FTWDS KSAM GNSFQRKLA+VKNG +P  +KPKRR  VSYGGRS++LPEKSQR+RS+SF
Sbjct: 716  FTWDSGKSAMQGNSFQRKLAIVKNGISPTPEKPKRRTPVSYGGRSSSLPEKSQRSRSMSF 775

Query: 1798 SPERVRVRGRSPAFNALAAAFENPNGRNLSTPPPVVKKLYPKS 1926
            SP+RVRVRGRSPAFNALAA FENPN RNLSTPPP+V+KLYPKS
Sbjct: 776  SPDRVRVRGRSPAFNALAANFENPNSRNLSTPPPMVRKLYPKS 818



 Score =  160 bits (406), Expect(2) = 0.0
 Identities = 79/112 (70%), Positives = 95/112 (84%), Gaps = 2/112 (1%)
 Frame = +3

Query: 2022 ESLIPRSVKVSPKAPITKP--DTESKENAMSSRIEALAIQEDVKEGEADDEEGLVTYPYE 2195
            E ++P++ KV+ +AP  KP  +T SKE AMSSRIEAL I+EDVKEGEA+DEEGL  YPYE
Sbjct: 846  EPVVPKTPKVTEEAPKPKPKPETNSKEKAMSSRIEALTIEEDVKEGEAEDEEGLPIYPYE 905

Query: 2196 RLTTLSADPVTDIDVTKRETYLSSQEFKEKFGMTRAAFYKLPKWKQNKLKMS 2351
            RL T S +PV +IDVTKRETYLSS+EF++KFGMT+ AFYKLPKWKQNKLKM+
Sbjct: 906  RLKTTSIEPVAEIDVTKRETYLSSEEFRQKFGMTKDAFYKLPKWKQNKLKMA 957



 Score = 90.5 bits (223), Expect = 2e-15
 Identities = 86/375 (22%), Positives = 162/375 (43%), Gaps = 17/375 (4%)
 Frame = +1

Query: 655  LQVWRVDVEGKTPLTSSDQSKFFCGDCYIFQYSYPGELGE-EYLVGTWFGEESVEEDRNS 831
            +++WR++     P+  S   KFF GD Y+   +   + G   + +  W G+++ +++  +
Sbjct: 21   IEIWRIENFRPMPVPKSSYGKFFTGDSYVILKTTALKNGALRHDIHYWLGKDTTQDEAGT 80

Query: 832  AISQASKMVESLKFLPVQARICEGNEXXXXXXXXXXXLVFK-GGVSDRYKKYIADKELRD 1008
            A  +  ++  +L    VQ R  +G+E           ++ + GGV+  +K   A+ E + 
Sbjct: 81   AAVKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIPQPGGVASGFKHAEAE-EHKT 139

Query: 1009 VTYSEDGLALFRVQGTGPENMQAIQVEAVASSLNSSYCYILHNGSSVFTWLGNLTTSEDQ 1188
              Y   G  +  V+          +V    SSLN    +IL   S +F + G+ ++ +++
Sbjct: 140  RLYVCKGKHVVHVK----------EVSFARSSLNHDDIFILDTKSKIFQFNGSNSSIQER 189

Query: 1189 ELVERLLDLIK----------PNMQSRPQKEGSESEQFWELLGGKSEYPSQKVGRD---V 1329
                 ++  IK           +++       +E+ +FW   GG +  P +    D   V
Sbjct: 190  AKALEVVQYIKDTYHDGKCEVASIEDGKLMADAETGEFWGFFGGFAPLPRKTANEDDKAV 249

Query: 1330 ESDPHLFSCSFSADLKVTEIHNFDQDDLMTEDIFILDCHSEIFVWVGQQVNTKYRKDALT 1509
            +S P    C      +  +  +  ++ L T   +ILDC  E+FVW+G+  +   RK A +
Sbjct: 250  DSLPAKLFCILKGQAEPVQADSLTRELLDTNKCYILDCGVEVFVWMGRNTSLDERKSASS 309

Query: 1510 IGEKFLARDFLLEKISSQAPIYIVMEGSEPTFF-TRFFTWDSTKSAMHGNSFQRKL-AMV 1683
              E+ L     L++  S   I  V+EG E   F ++F  W  T +       + K+ A++
Sbjct: 310  AAEELLRS---LDRPKSH--IIRVIEGFETVMFRSKFDMWPETTAVTVSEDGRGKVAALL 364

Query: 1684 KNGGTPVLDKPKRRP 1728
            K  G  V    K  P
Sbjct: 365  KRQGVNVKGLLKAAP 379


>ref|XP_002329670.1| predicted protein [Populus trichocarpa] gi|222870551|gb|EEF07682.1|
            predicted protein [Populus trichocarpa]
          Length = 977

 Score =  986 bits (2549), Expect(2) = 0.0
 Identities = 488/653 (74%), Positives = 556/653 (85%), Gaps = 11/653 (1%)
 Frame = +1

Query: 1    IFQFNGSNSSIQERAKALEVVQYIKDTYHDGKCEIAAIEDGKLMADADTGEFWGLFGGFA 180
            IFQFNGSNSSIQERAKALEVVQYIKDTYHDGKCE+AA+EDGKLMADA+TGEFWG FGGFA
Sbjct: 183  IFQFNGSNSSIQERAKALEVVQYIKDTYHDGKCEVAAVEDGKLMADAETGEFWGFFGGFA 242

Query: 181  PLPRKTVTEDANNTGDISTRLFSVVKGKHEPVDVDSLKRELLDTYKCYLLDCGSEVFVWM 360
            PLPRKT +++      +ST+LF V KG+ EPV+ DSL RE LDT KCY+LDCG+EVFVWM
Sbjct: 243  PLPRKTASDEDKTDVSLSTKLFCVEKGQAEPVETDSLTREFLDTNKCYILDCGAEVFVWM 302

Query: 361  GRNTSLDERKSASEAAEELLRTIPRPKTHIIRVMEGFETVMFRSKFDSWPQSTDVAVSED 540
            GRNT LDERKSAS AAEEL+R + RPK+ ++RV+EGFETVMFRSKF+SWPQ+T+V VSED
Sbjct: 303  GRNTPLDERKSASVAAEELVRAVERPKSRVVRVIEGFETVMFRSKFESWPQTTNVTVSED 362

Query: 541  GRGKVAALLKRQGVNVKGLLKATPAKEEPQPYIDCTGNLQVWRVDVEGKTPLTSSDQSKF 720
            GRGKVAALL+RQGVNVKGLLK  PAKEEPQPYID TGNLQVW V+ + K  + ++DQSKF
Sbjct: 363  GRGKVAALLRRQGVNVKGLLKTAPAKEEPQPYIDVTGNLQVWSVNGQEKVLIPAADQSKF 422

Query: 721  FCGDCYIFQYSYPGELGEEYLVGTWFGEESVEEDRNSAISQASKMVESLKFLPVQARICE 900
            + G CYIFQYSYPGE  EEYL+GTWFG++SV+E+R SAIS  SKMVESLKFLP QARI E
Sbjct: 423  YSGGCYIFQYSYPGEDREEYLIGTWFGKKSVKEERASAISLVSKMVESLKFLPAQARIYE 482

Query: 901  GNEXXXXXXXXXXXLVFKGGVSDRYKKYIADKELRDVTYSEDGLALFRVQGTGPENMQAI 1080
            GNE           LVFKGG S  YK YI + EL D TY E+G+ALFRVQG+GP+NMQA+
Sbjct: 483  GNEPIQFFSIFQSFLVFKGGHSSGYKNYIEENELPDETYKEEGIALFRVQGSGPDNMQAL 542

Query: 1081 QVEAVASSLNSSYCYILHNGSSVFTWLGNLTTSEDQELVERLLDLIKPNMQSRPQKEGSE 1260
            QVE VASSLNSSYCYILHN SSVFTW GNLT+SEDQEL+ER LDLIKPNMQS+PQKEGSE
Sbjct: 543  QVEPVASSLNSSYCYILHNDSSVFTWSGNLTSSEDQELIERQLDLIKPNMQSKPQKEGSE 602

Query: 1261 SEQFWELLGGKSEYPSQKVGRDVESDPHLFSCSFSAD-----------LKVTEIHNFDQD 1407
            +E FW+LLGGKSEYPSQK+ R+ ESDPHLFSC FS             L+V+EI+NF QD
Sbjct: 603  AEHFWDLLGGKSEYPSQKLAREGESDPHLFSCIFSKVLCGGYYNKFLLLQVSEIYNFTQD 662

Query: 1408 DLMTEDIFILDCHSEIFVWVGQQVNTKYRKDALTIGEKFLARDFLLEKISSQAPIYIVME 1587
            DLMTEDIFILD HSEIFVWVGQQV++K +  ALTIGEKFL  DFLLEK+SS+ PIYIVME
Sbjct: 663  DLMTEDIFILDSHSEIFVWVGQQVDSKSKLQALTIGEKFLEHDFLLEKLSSETPIYIVME 722

Query: 1588 GSEPTFFTRFFTWDSTKSAMHGNSFQRKLAMVKNGGTPVLDKPKRRPAVSYGGRSAALPE 1767
            GSEP FFTRFFTWDS KS MHGNSFQRKLA+VKNGGT +LDKPKRR  VS+GGRS ++P+
Sbjct: 723  GSEPPFFTRFFTWDSAKSLMHGNSFQRKLAIVKNGGTTLLDKPKRRTPVSHGGRS-SVPD 781

Query: 1768 KSQRARSVSFSPERVRVRGRSPAFNALAAAFENPNGRNLSTPPPVVKKLYPKS 1926
            KSQR+RS+SFSP+RVRVRGRSPAF+ALAA FE+P+ RNLSTPPPVV+K+YPKS
Sbjct: 782  KSQRSRSMSFSPDRVRVRGRSPAFSALAANFESPSARNLSTPPPVVRKVYPKS 834



 Score =  160 bits (404), Expect(2) = 0.0
 Identities = 77/108 (71%), Positives = 91/108 (84%)
 Frame = +3

Query: 2028 LIPRSVKVSPKAPITKPDTESKENAMSSRIEALAIQEDVKEGEADDEEGLVTYPYERLTT 2207
            ++PRSVK SP+AP   P++ SKEN+MSSRIE+L IQEDVKE EA+DEEGL  YPYE L  
Sbjct: 866  IMPRSVKASPEAPKLTPESNSKENSMSSRIESLTIQEDVKEDEAEDEEGLPIYPYESLKV 925

Query: 2208 LSADPVTDIDVTKRETYLSSQEFKEKFGMTRAAFYKLPKWKQNKLKMS 2351
             S+DP T+IDVTKRETYLS+ EF+EKFGM + AFYKLPKWKQNKLKM+
Sbjct: 926  NSSDPATEIDVTKRETYLSAVEFREKFGMAKYAFYKLPKWKQNKLKMA 973



 Score = 88.2 bits (217), Expect = 1e-14
 Identities = 85/381 (22%), Positives = 163/381 (42%), Gaps = 22/381 (5%)
 Frame = +1

Query: 655  LQVWRVDVEGKTPLTSSDQSKFFCGDCYIFQYSYPGELGE-EYLVGTWFGEESVEEDRNS 831
            L++WR++     P+  S   KFF GD Y+   +   + G   + +  W G+++ +++  +
Sbjct: 21   LEIWRIENFRPVPVPKSSHGKFFTGDSYVILQTTALKSGSLRHDIHYWLGKDTSQDEAGA 80

Query: 832  AISQASKMVESLKFLPVQARICEGNEXXXXXXXXXXXLV-FKGGVSDRYKKYIADKELRD 1008
            A  +  ++  +L    VQ R  +G+E           ++  KGGV+  +K   A+     
Sbjct: 81   AAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIPQKGGVASGFKHPEAE----- 135

Query: 1009 VTYSEDGLALFRVQG-----TGPENMQAIQVEAVASSLNSSYCYILHNGSSVFTWLGNLT 1173
                E    LF   G         +++   V    SSLN    +IL   S +F + G+ +
Sbjct: 136  ----EHQTCLFVCTGKHVVHVNEASLKFDFVPFARSSLNHDDIFILDTKSKIFQFNGSNS 191

Query: 1174 TSEDQELVERLLDLIKPN----------MQSRPQKEGSESEQFWELLGGKSEYPSQKVGR 1323
            + +++     ++  IK            ++       +E+ +FW   GG +  P +    
Sbjct: 192  SIQERAKALEVVQYIKDTYHDGKCEVAAVEDGKLMADAETGEFWGFFGGFAPLPRKTASD 251

Query: 1324 DVESDPHLFS---CSFSADLKVTEIHNFDQDDLMTEDIFILDCHSEIFVWVGQQVNTKYR 1494
            + ++D  L +   C      +  E  +  ++ L T   +ILDC +E+FVW+G+      R
Sbjct: 252  EDKTDVSLSTKLFCVEKGQAEPVETDSLTREFLDTNKCYILDCGAEVFVWMGRNTPLDER 311

Query: 1495 KDALTIGEKFLARDFLLEKISSQAPIYIVMEGSEPTFF-TRFFTWDSTKSAMHGNSFQRK 1671
            K A    E+ +     +E+  S+  +  V+EG E   F ++F +W  T +       + K
Sbjct: 312  KSASVAAEELVR---AVERPKSR--VVRVIEGFETVMFRSKFESWPQTTNVTVSEDGRGK 366

Query: 1672 L-AMVKNGGTPVLDKPKRRPA 1731
            + A+++  G  V    K  PA
Sbjct: 367  VAALLRRQGVNVKGLLKTAPA 387


>ref|XP_002324461.1| predicted protein [Populus trichocarpa] gi|222865895|gb|EEF03026.1|
            predicted protein [Populus trichocarpa]
          Length = 961

 Score =  977 bits (2526), Expect(2) = 0.0
 Identities = 494/655 (75%), Positives = 550/655 (83%), Gaps = 13/655 (1%)
 Frame = +1

Query: 1    IFQFNGSNSSIQERAKALEVVQYIKDTYHDGKCEIAAIEDGKLMADADTGEFWGLFGGFA 180
            IFQFNGSNSSIQERAKALEVVQYIKDTYHDGKCE+AA+EDGKLMADA+TGEFWG FGGFA
Sbjct: 173  IFQFNGSNSSIQERAKALEVVQYIKDTYHDGKCEVAAVEDGKLMADAETGEFWGFFGGFA 232

Query: 181  PLPRKTVTEDANNTGDISTRLF--SVVKGKHEPVDVDSLKRELLDTYKCYLLDCGSEVFV 354
            PLPRKT          ++  L   SV KG+ EPV+ DSL RELLDT KCY+LDCG EVFV
Sbjct: 233  PLPRKTTI--------LTNYLLHESVEKGQAEPVEADSLTRELLDTNKCYILDCGIEVFV 284

Query: 355  WMGRNTSLDERKSASEAAEELLRTIPRPKTHIIRVMEGFETVMFRSKFDSWPQSTDVAVS 534
            WMGRNTSLDERKSAS AAEEL+R   RP + I RV+EGFETVMFRSKF+SWPQ+T+V VS
Sbjct: 285  WMGRNTSLDERKSASGAAEELVRAAERPNSRIARVIEGFETVMFRSKFESWPQTTNVTVS 344

Query: 535  EDGRGKVAALLKRQGVNVKGLLKATPAKEEPQPYIDCTGNLQVWRVDVEGKTPLTSSDQS 714
            EDGRGKVAALL+RQGVNV GLLK  P KEEPQPYID TGNLQVW V+ + K  + +++QS
Sbjct: 345  EDGRGKVAALLRRQGVNVNGLLKTAPVKEEPQPYIDVTGNLQVWSVNDQEKILIPAANQS 404

Query: 715  KFFCGDCYIFQYSYPGELGEEYLVGTWFGEESVEEDRNSAISQASKMVESLKFLPVQARI 894
            KF+ G CYIFQYSYPGE  EEYL+GTWFG++SVEE+R SAIS ASKMVESLKFLP QARI
Sbjct: 405  KFYSGGCYIFQYSYPGEDREEYLIGTWFGKKSVEEERASAISLASKMVESLKFLPAQARI 464

Query: 895  CEGNEXXXXXXXXXXXLVFKGGVSDRYKKYIADKELRDVTYSEDGLALFRVQGTGPENMQ 1074
             EGNE           +VFKGG S  YKKYIA+ EL D T  EDG+ALFRVQG+GP+NMQ
Sbjct: 465  FEGNEPIQFFSIFQSFIVFKGGHSSGYKKYIAENELPDETCKEDGVALFRVQGSGPDNMQ 524

Query: 1075 AIQVEAVASSLNSSYCYILHNGSSVFTWLGNLTTSEDQELVERLLDLIKPNMQSRPQKEG 1254
            AIQVE VASSLNSSYCYILHN SSVFTW GNLTTSEDQEL+ER LDLIKPNMQS+PQKEG
Sbjct: 525  AIQVEPVASSLNSSYCYILHNDSSVFTWSGNLTTSEDQELIERQLDLIKPNMQSKPQKEG 584

Query: 1255 SESEQFWELLGGKSEYPSQKVGRDVESDPHLFSCSF-----------SADLKVTEIHNFD 1401
            SESEQFW+LLGGKSEYPSQK+ R+ ESDPHLFSC F              L+V+EI+NF 
Sbjct: 585  SESEQFWDLLGGKSEYPSQKLAREAESDPHLFSCIFLKVLCVGFYNKFLSLQVSEIYNFT 644

Query: 1402 QDDLMTEDIFILDCHSEIFVWVGQQVNTKYRKDALTIGEKFLARDFLLEKISSQAPIYIV 1581
            QDDLMTEDIFILD HSEIFVWVGQQV++K +  AL+IGEKFL  DFLL+K S + PIYIV
Sbjct: 645  QDDLMTEDIFILDTHSEIFVWVGQQVDSKSKLQALSIGEKFLEHDFLLKKSSGETPIYIV 704

Query: 1582 MEGSEPTFFTRFFTWDSTKSAMHGNSFQRKLAMVKNGGTPVLDKPKRRPAVSYGGRSAAL 1761
            MEGSEP FFTRFFTWDS KS+MHGNSFQRKLA+VKNGGTP+LDKPKRR AVSYGGRS ++
Sbjct: 705  MEGSEPPFFTRFFTWDSAKSSMHGNSFQRKLAIVKNGGTPLLDKPKRRTAVSYGGRS-SV 763

Query: 1762 PEKSQRARSVSFSPERVRVRGRSPAFNALAAAFENPNGRNLSTPPPVVKKLYPKS 1926
            P+KSQR+RS+SFSP+RVRVRGRSPAFNALAA FENPN RNLSTPPPVV+K+YPKS
Sbjct: 764  PDKSQRSRSMSFSPDRVRVRGRSPAFNALAANFENPNARNLSTPPPVVRKVYPKS 818



 Score =  157 bits (397), Expect(2) = 0.0
 Identities = 76/108 (70%), Positives = 90/108 (83%)
 Frame = +3

Query: 2028 LIPRSVKVSPKAPITKPDTESKENAMSSRIEALAIQEDVKEGEADDEEGLVTYPYERLTT 2207
            ++PRSVKVSP+ P + P++ SKE  +S RIE+L IQEDVKEGEA+DEEGL  YPYE L  
Sbjct: 850  IMPRSVKVSPETPKSTPESNSKEKPISIRIESLTIQEDVKEGEAEDEEGLPIYPYEGLKV 909

Query: 2208 LSADPVTDIDVTKRETYLSSQEFKEKFGMTRAAFYKLPKWKQNKLKMS 2351
             S DPVT+IDVTKRETYLS+ EF+EKFGM + AFYKLPKWKQNKLKM+
Sbjct: 910  NSPDPVTEIDVTKRETYLSAAEFREKFGMAKDAFYKLPKWKQNKLKMA 957



 Score = 80.5 bits (197), Expect = 2e-12
 Identities = 84/387 (21%), Positives = 160/387 (41%), Gaps = 13/387 (3%)
 Frame = +1

Query: 655  LQVWRVDVEGKTPLTSSDQSKFFCGDCYIFQYSYPGELGE-EYLVGTWFGEESVEEDRNS 831
            L++WR++     P+  S   KFF GD Y+   +   + G   + +  W G+++ +++  +
Sbjct: 21   LEIWRIENFRPVPVPKSSHGKFFTGDSYVILQTTALKSGSLRHDIHYWLGKDTSQDEAGA 80

Query: 832  AISQASKMVESLKFLPVQARICEGNEXXXXXXXXXXXLV-FKGGVSDRYKKYIADKELRD 1008
            A  +  ++  +L    VQ R  +G+E           ++  +GGV+  +K+  A      
Sbjct: 81   AAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIPQEGGVASGFKQAEA------ 134

Query: 1009 VTYSEDGLALFRVQGTGPENMQAIQVEAVASSLNSSYCYILHNGSSVFTWLGNLTTSEDQ 1188
                E    LF  +G        + V    SSLN    +IL   S +F + G+ ++ +++
Sbjct: 135  ---MEHQTHLFVCRG-----KHVVHVPFARSSLNHDDIFILDTKSKIFQFNGSNSSIQER 186

Query: 1189 ELVERLLDLIKPN----------MQSRPQKEGSESEQFWELLGGKSEYPSQKVGRDVESD 1338
                 ++  IK            ++       +E+ +FW   GG +  P +     + ++
Sbjct: 187  AKALEVVQYIKDTYHDGKCEVAAVEDGKLMADAETGEFWGFFGGFAPLPRKTT---ILTN 243

Query: 1339 PHLFSCSFSADLKVTEIHNFDQDDLMTEDIFILDCHSEIFVWVGQQVNTKYRKDALTIGE 1518
              L         +  E  +  ++ L T   +ILDC  E+FVW+G+  +   RK A    E
Sbjct: 244  YLLHESVEKGQAEPVEADSLTRELLDTNKCYILDCGIEVFVWMGRNTSLDERKSASGAAE 303

Query: 1519 KFLARDFLLEKISSQAPIYIVMEGSEPTFF-TRFFTWDSTKSAMHGNSFQRKLAMVKNGG 1695
            + +      E+ +S+  I  V+EG E   F ++F +W  T +       + K+A +    
Sbjct: 304  ELVR---AAERPNSR--IARVIEGFETVMFRSKFESWPQTTNVTVSEDGRGKVAAL---- 354

Query: 1696 TPVLDKPKRRPAVSYGGRSAALPEKSQ 1776
                    RR  V+  G     P K +
Sbjct: 355  -------LRRQGVNVNGLLKTAPVKEE 374


>ref|XP_003533726.1| PREDICTED: villin-4-like [Glycine max]
          Length = 960

 Score =  967 bits (2499), Expect(2) = 0.0
 Identities = 475/645 (73%), Positives = 550/645 (85%), Gaps = 3/645 (0%)
 Frame = +1

Query: 1    IFQFNGSNSSIQERAKALEVVQYIKDTYHDGKCEIAAIEDGKLMADADTGEFWGLFGGFA 180
            IFQFNGSNSSIQERAKALEVVQYIKDTYH+GKCE+AA+EDGKLMAD +TGEFWG FGGFA
Sbjct: 176  IFQFNGSNSSIQERAKALEVVQYIKDTYHEGKCEVAAVEDGKLMADPETGEFWGFFGGFA 235

Query: 181  PLPRKTVTEDANNTGDISTRLFSVVKGKHEPVDVDSLKRELLDTYKCYLLDCGSEVFVWM 360
            PLPRKT ++D   T     +L  V KG+ EPV+ DSLKRELLDT KCY+LDCG EVFVW+
Sbjct: 236  PLPRKTASDDDKPTDSRPPKLLCVEKGQAEPVETDSLKRELLDTNKCYILDCGFEVFVWL 295

Query: 361  GRNTSLDERKSASEAAEELLRTIPRPKTHIIRVMEGFETVMFRSKFDSWPQSTDVAVSED 540
            GRNTSLDERKSAS  A+E++    + K  IIRV+EGFETVMFRSKFDSWPQ+TDV VSED
Sbjct: 296  GRNTSLDERKSASGVADEIVSGTDQLKPQIIRVIEGFETVMFRSKFDSWPQTTDVTVSED 355

Query: 541  GRGKVAALLKRQGVNVKGLLKATPAKEEPQPYIDCTGNLQVWRVDVEGKTPLTSSDQSKF 720
            GRGKVAALLKRQGVNVKGLLKA P +EEPQP+IDCTG+LQVW V+ + K  L +SDQSKF
Sbjct: 356  GRGKVAALLKRQGVNVKGLLKADPVREEPQPHIDCTGHLQVWHVNGQEKILLQASDQSKF 415

Query: 721  FCGDCYIFQYSYPGELGEEYLVGTWFGEESVEEDRNSAISQASKMVESLKFLPVQARICE 900
            + GDC+IFQY+YPGE  E+ L+GTW G+ SVEE+R SA S ASKMVES+KFL  QARI E
Sbjct: 416  YSGDCFIFQYTYPGEDKEDCLIGTWIGKNSVEEERASANSLASKMVESMKFLASQARIYE 475

Query: 901  GNEXXXXXXXXXXXLVFKGGVSDRYKKYIADKELRDVTYSEDGLALFRVQGTGPENMQAI 1080
            GNE           +VFKGG+S+ YK YIA KE+ D TY+E+G+ALFR+QG+GP+NMQAI
Sbjct: 476  GNEPIQFHSILQSFIVFKGGISEGYKTYIAQKEIPDDTYNENGVALFRIQGSGPDNMQAI 535

Query: 1081 QVEAVASSLNSSYCYILHNGSSVFTWLGNLTTSEDQELVERLLDLIKPNMQSRPQKEGSE 1260
            QVE VASSLNSSYCYILHNG +VFTW GN T++E+QELVER+LDLIKPN+QS+PQ+EGSE
Sbjct: 536  QVEPVASSLNSSYCYILHNGPAVFTWSGNSTSAENQELVERMLDLIKPNLQSKPQREGSE 595

Query: 1261 SEQFWELLGGKSEYPSQKVGRDVESDPHLFSCSFS-ADLKVTEIHNFDQDDLMTEDIFIL 1437
            SEQFW+LLGGKSEYPSQK+ R+ ESDPHLFSC FS  +LKVTE++NF QDDLMTEDIF+L
Sbjct: 596  SEQFWDLLGGKSEYPSQKILREPESDPHLFSCHFSKGNLKVTEVYNFSQDDLMTEDIFVL 655

Query: 1438 DCHSEIFVWVGQQVNTKYRKDALTIGEKFLARDFLLEKISSQAPIYIVMEGSEPTFFTRF 1617
            DCHSEIFVWVGQQV++K R  AL+IGEKFL  DFLLEK+S  APIY+VMEGSEP FFTRF
Sbjct: 656  DCHSEIFVWVGQQVDSKSRMQALSIGEKFLEHDFLLEKLSRVAPIYVVMEGSEPPFFTRF 715

Query: 1618 FTWDSTKSAMHGNSFQRKLAMVKNGGTPVLDKPKRRPAVSYGGRSAALPEKSQR--ARSV 1791
            F WDS K+AM GNSFQRKL +VK+GG PVLDKPKRR + SYGGRS+++P+KS +  +RS+
Sbjct: 716  FKWDSAKAAMLGNSFQRKLTIVKSGGAPVLDKPKRRTSASYGGRSSSVPDKSSQRSSRSM 775

Query: 1792 SFSPERVRVRGRSPAFNALAAAFENPNGRNLSTPPPVVKKLYPKS 1926
            S SP+RVRVRGRSPAFNALAA FENPN RNLSTPPPV++KLYPKS
Sbjct: 776  SVSPDRVRVRGRSPAFNALAANFENPNSRNLSTPPPVIRKLYPKS 820



 Score =  157 bits (396), Expect(2) = 0.0
 Identities = 76/110 (69%), Positives = 92/110 (83%)
 Frame = +3

Query: 2022 ESLIPRSVKVSPKAPITKPDTESKENAMSSRIEALAIQEDVKEGEADDEEGLVTYPYERL 2201
            E++IPRS+KV PK+    P+   KEN++S+R+E+L IQEDVKE E +DEEGLV YPYERL
Sbjct: 850  ETMIPRSLKVMPKS---NPEKNDKENSVSTRVESLTIQEDVKEDEVEDEEGLVIYPYERL 906

Query: 2202 TTLSADPVTDIDVTKRETYLSSQEFKEKFGMTRAAFYKLPKWKQNKLKMS 2351
              +S DPV +IDVTKRETYLSS EFKEKFGM++ AFYKLPKWKQNKLKM+
Sbjct: 907  KIMSTDPVPNIDVTKRETYLSSAEFKEKFGMSKDAFYKLPKWKQNKLKMA 956



 Score = 89.7 bits (221), Expect = 4e-15
 Identities = 83/375 (22%), Positives = 159/375 (42%), Gaps = 17/375 (4%)
 Frame = +1

Query: 655  LQVWRVDVEGKTPLTSSDQSKFFCGDCYIFQYSYPGELGE-EYLVGTWFGEESVEEDRNS 831
            L++WR++     P+  S   KFF GD Y+   +   + G   + +  W G+++ +++  +
Sbjct: 21   LEIWRIENFNPVPVPKSSYGKFFTGDSYVILKTTASKSGALRHDIHYWLGKDTSQDEAGA 80

Query: 832  AISQASKMVESLKFLPVQARICEGNEXXXXXXXXXXXLV-FKGGVSDRYKKYIADKELRD 1008
            A  +  ++  +L    VQ R  +G+E           ++  +GGVS  +K   A+K    
Sbjct: 81   AAIKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIPQEGGVSSGFKHPEAEKH--- 137

Query: 1009 VTYSEDGLALFRVQGTGPENMQAIQVEAVASSLNSSYCYILHNGSSVFTWLGNLTTSEDQ 1188
                     LF  +G    +++  +V    +SLN    ++L   S +F + G+ ++ +++
Sbjct: 138  ------KTRLFVCRGKHVVHVK--EVPFARASLNHDDIFVLDTESKIFQFNGSNSSIQER 189

Query: 1189 ELVERLLDLIKPN----------MQSRPQKEGSESEQFWELLGGKSEYPSQKVGRD---V 1329
                 ++  IK            ++        E+ +FW   GG +  P +    D    
Sbjct: 190  AKALEVVQYIKDTYHEGKCEVAAVEDGKLMADPETGEFWGFFGGFAPLPRKTASDDDKPT 249

Query: 1330 ESDPHLFSCSFSADLKVTEIHNFDQDDLMTEDIFILDCHSEIFVWVGQQVNTKYRKDALT 1509
            +S P    C      +  E  +  ++ L T   +ILDC  E+FVW+G+  +   RK A  
Sbjct: 250  DSRPPKLLCVEKGQAEPVETDSLKRELLDTNKCYILDCGFEVFVWLGRNTSLDERKSASG 309

Query: 1510 IGEKFLARDFLLEKISSQAPIYIVMEGSEPTFF-TRFFTWDSTKSAMHGNSFQRKL-AMV 1683
            + ++ ++    L     +  I  V+EG E   F ++F +W  T         + K+ A++
Sbjct: 310  VADEIVSGTDQL-----KPQIIRVIEGFETVMFRSKFDSWPQTTDVTVSEDGRGKVAALL 364

Query: 1684 KNGGTPVLDKPKRRP 1728
            K  G  V    K  P
Sbjct: 365  KRQGVNVKGLLKADP 379