BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00006770
         (1415 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002273902.2| PREDICTED: ubiquitin-like modifier-activatin...   790   0.0  
emb|CBI30675.3| unnamed protein product [Vitis vinifera]              790   0.0  
ref|XP_002522565.1| autophagy protein, putative [Ricinus communi...   737   0.0  
ref|NP_001242518.1| ubiquitin-like modifier-activating enzyme at...   729   0.0  
ref|XP_002305077.1| predicted protein [Populus trichocarpa] gi|2...   695   0.0  

>ref|XP_002273902.2| PREDICTED: ubiquitin-like modifier-activating enzyme atg7-like [Vitis
            vinifera]
          Length = 711

 Score =  790 bits (2040), Expect = 0.0
 Identities = 372/470 (79%), Positives = 421/470 (89%)
 Frame = -1

Query: 1412 MAGHENGPILQFAPFQSAVDEGFWHRLSSLKLNQLGIDESPISVTGFYAPCSHSQVSNHL 1233
            MAG E+G +LQFAPFQS+VDEGFWHRLSSLKLN+LGID+SPIS+TGFYAPCSHSQVSNHL
Sbjct: 1    MAGQESGAVLQFAPFQSSVDEGFWHRLSSLKLNKLGIDDSPISITGFYAPCSHSQVSNHL 60

Query: 1232 TLLAESLPPEPXXXXXXXXXXSGNRNRCSIPGILYNTNTLEGFHALDKQSLLKAEAKKIW 1053
            TLLAESLPPEP           GNRNRCS+PGILYNTNTLE FHALDKQSLLKAEA+KIW
Sbjct: 61   TLLAESLPPEPSEQSSTPPISRGNRNRCSVPGILYNTNTLESFHALDKQSLLKAEAEKIW 120

Query: 1052 EDIHSGKAEEESSVLSRFLLISFADLKKWKFYYWFAFPALVLDPPATITSLKPAAQCFSP 873
             DIHSG+AEE+ SVLSRFLLISFADLKKW F+YWFAFPALVLDPPAT+  LKPA+Q FS 
Sbjct: 121  NDIHSGRAEEDCSVLSRFLLISFADLKKWSFHYWFAFPALVLDPPATLVDLKPASQWFSL 180

Query: 872  EEAESLAAVCNEWRNSSSTADVPFFLVSIDLNTHASIRHLKDWEACRSDDHEYLFGFYDP 693
            EEAES++A CNEWRNSSSTADVPFFLVSI  N+ A+IRHLKDWEAC+ D H+ LFGFYDP
Sbjct: 181  EEAESVSAACNEWRNSSSTADVPFFLVSIASNSRATIRHLKDWEACQEDGHKLLFGFYDP 240

Query: 692  CHLPNNPGWPLRNFIAFICSRWNLQKIRFFCYREHRGFADMKMSLIGEALISVSQEWKHH 513
            CHLP NPGWPLRNF+  I SRWN++K++F CYRE+RGFAD+ +SL+GEA IS  QEW+  
Sbjct: 241  CHLPKNPGWPLRNFLVLIYSRWNIKKVQFLCYRENRGFADLGLSLVGEAQISAPQEWRDE 300

Query: 512  KHVPNAVGWEVNKGKKISRCITLANSMDPTKLAVSAADLNLKLMRWRALPSLDLNMLSAT 333
             HVPN VGWE+N+GKK+ RCI+LA SMDPT+LA+SAADLNLKLMRWRALPSL+LN+LS  
Sbjct: 301  LHVPNVVGWELNRGKKVYRCISLAKSMDPTRLAISAADLNLKLMRWRALPSLNLNILSTM 360

Query: 332  RCLLLGAGTLGCQVARMLMAWGVRKITLVDSGKVAMSNPLRQSLYTLDDCLNGGEYKALA 153
            +CLLLGAGTLGCQVARMLMAWGVRKITL+D+GKVAMSNPLRQSLYTLDDCLNGGE+KALA
Sbjct: 361  KCLLLGAGTLGCQVARMLMAWGVRKITLLDNGKVAMSNPLRQSLYTLDDCLNGGEFKALA 420

Query: 152  AVKSLKQIFPAVEADGIVMAIPMPGHPVPSQDENNVLEDCRRLQDLIDSH 3
            AV SLK+IFPAVEA+G+VMAIPMPGHPVPSQ+E +VL+DCR L +LI+SH
Sbjct: 421  AVNSLKRIFPAVEAEGVVMAIPMPGHPVPSQEEESVLDDCRCLCELIESH 470


>emb|CBI30675.3| unnamed protein product [Vitis vinifera]
          Length = 787

 Score =  790 bits (2040), Expect = 0.0
 Identities = 372/470 (79%), Positives = 421/470 (89%)
 Frame = -1

Query: 1412 MAGHENGPILQFAPFQSAVDEGFWHRLSSLKLNQLGIDESPISVTGFYAPCSHSQVSNHL 1233
            MAG E+G +LQFAPFQS+VDEGFWHRLSSLKLN+LGID+SPIS+TGFYAPCSHSQVSNHL
Sbjct: 77   MAGQESGAVLQFAPFQSSVDEGFWHRLSSLKLNKLGIDDSPISITGFYAPCSHSQVSNHL 136

Query: 1232 TLLAESLPPEPXXXXXXXXXXSGNRNRCSIPGILYNTNTLEGFHALDKQSLLKAEAKKIW 1053
            TLLAESLPPEP           GNRNRCS+PGILYNTNTLE FHALDKQSLLKAEA+KIW
Sbjct: 137  TLLAESLPPEPSEQSSTPPISRGNRNRCSVPGILYNTNTLESFHALDKQSLLKAEAEKIW 196

Query: 1052 EDIHSGKAEEESSVLSRFLLISFADLKKWKFYYWFAFPALVLDPPATITSLKPAAQCFSP 873
             DIHSG+AEE+ SVLSRFLLISFADLKKW F+YWFAFPALVLDPPAT+  LKPA+Q FS 
Sbjct: 197  NDIHSGRAEEDCSVLSRFLLISFADLKKWSFHYWFAFPALVLDPPATLVDLKPASQWFSL 256

Query: 872  EEAESLAAVCNEWRNSSSTADVPFFLVSIDLNTHASIRHLKDWEACRSDDHEYLFGFYDP 693
            EEAES++A CNEWRNSSSTADVPFFLVSI  N+ A+IRHLKDWEAC+ D H+ LFGFYDP
Sbjct: 257  EEAESVSAACNEWRNSSSTADVPFFLVSIASNSRATIRHLKDWEACQEDGHKLLFGFYDP 316

Query: 692  CHLPNNPGWPLRNFIAFICSRWNLQKIRFFCYREHRGFADMKMSLIGEALISVSQEWKHH 513
            CHLP NPGWPLRNF+  I SRWN++K++F CYRE+RGFAD+ +SL+GEA IS  QEW+  
Sbjct: 317  CHLPKNPGWPLRNFLVLIYSRWNIKKVQFLCYRENRGFADLGLSLVGEAQISAPQEWRDE 376

Query: 512  KHVPNAVGWEVNKGKKISRCITLANSMDPTKLAVSAADLNLKLMRWRALPSLDLNMLSAT 333
             HVPN VGWE+N+GKK+ RCI+LA SMDPT+LA+SAADLNLKLMRWRALPSL+LN+LS  
Sbjct: 377  LHVPNVVGWELNRGKKVYRCISLAKSMDPTRLAISAADLNLKLMRWRALPSLNLNILSTM 436

Query: 332  RCLLLGAGTLGCQVARMLMAWGVRKITLVDSGKVAMSNPLRQSLYTLDDCLNGGEYKALA 153
            +CLLLGAGTLGCQVARMLMAWGVRKITL+D+GKVAMSNPLRQSLYTLDDCLNGGE+KALA
Sbjct: 437  KCLLLGAGTLGCQVARMLMAWGVRKITLLDNGKVAMSNPLRQSLYTLDDCLNGGEFKALA 496

Query: 152  AVKSLKQIFPAVEADGIVMAIPMPGHPVPSQDENNVLEDCRRLQDLIDSH 3
            AV SLK+IFPAVEA+G+VMAIPMPGHPVPSQ+E +VL+DCR L +LI+SH
Sbjct: 497  AVNSLKRIFPAVEAEGVVMAIPMPGHPVPSQEEESVLDDCRCLCELIESH 546


>ref|XP_002522565.1| autophagy protein, putative [Ricinus communis]
            gi|223538256|gb|EEF39865.1| autophagy protein, putative
            [Ricinus communis]
          Length = 710

 Score =  737 bits (1903), Expect = 0.0
 Identities = 343/464 (73%), Positives = 410/464 (88%)
 Frame = -1

Query: 1394 GPILQFAPFQSAVDEGFWHRLSSLKLNQLGIDESPISVTGFYAPCSHSQVSNHLTLLAES 1215
            G ILQFAPFQS+VDEGFWHRLSSLKLN+ GID+SPI +TGFYAPCSHSQVSNHLT+LAES
Sbjct: 10   GSILQFAPFQSSVDEGFWHRLSSLKLNKFGIDDSPIPITGFYAPCSHSQVSNHLTVLAES 69

Query: 1214 LPPEPXXXXXXXXXXSGNRNRCSIPGILYNTNTLEGFHALDKQSLLKAEAKKIWEDIHSG 1035
            L  +             NRNRC++PGILYNTNTLE +H+LDK++LLK EA KIW+DIH+G
Sbjct: 70   LSCDESESEECSIQVLSNRNRCAVPGILYNTNTLEAYHSLDKKTLLKKEANKIWDDIHTG 129

Query: 1034 KAEEESSVLSRFLLISFADLKKWKFYYWFAFPALVLDPPATITSLKPAAQCFSPEEAESL 855
            KA E+SSVL RF+++SFADLKKW F+Y FAFPALVLDPPAT+  LKPA+QCF+ +EA+SL
Sbjct: 130  KAVEDSSVLCRFVVVSFADLKKWSFHYCFAFPALVLDPPATVVDLKPASQCFTVQEAQSL 189

Query: 854  AAVCNEWRNSSSTADVPFFLVSIDLNTHASIRHLKDWEACRSDDHEYLFGFYDPCHLPNN 675
             A CN+WRNSS TADVPFFLV ID N+HA+IRHL +WEAC+SDD + LFGFYDPCHLPNN
Sbjct: 190  LAACNDWRNSSLTADVPFFLVCIDSNSHATIRHLNNWEACQSDDQKVLFGFYDPCHLPNN 249

Query: 674  PGWPLRNFIAFICSRWNLQKIRFFCYREHRGFADMKMSLIGEALISVSQEWKHHKHVPNA 495
            PGWPLRNF+A ICSRWN + +RF CYRE+RGFAD+ +SL+GEAL+++ Q W   + VPNA
Sbjct: 250  PGWPLRNFLALICSRWNPKIVRFLCYRENRGFADLGLSLVGEALLTLPQGWADPQCVPNA 309

Query: 494  VGWEVNKGKKISRCITLANSMDPTKLAVSAADLNLKLMRWRALPSLDLNMLSATRCLLLG 315
            VGWE+NKG++ISR I LANS+DPT+LAVSAADLNLKLMRWRALPSL+L+ LS+++CLLLG
Sbjct: 310  VGWELNKGRQISRVIHLANSLDPTRLAVSAADLNLKLMRWRALPSLNLDKLSSSKCLLLG 369

Query: 314  AGTLGCQVARMLMAWGVRKITLVDSGKVAMSNPLRQSLYTLDDCLNGGEYKALAAVKSLK 135
            AGTLGCQVARMLM+WGVR+ITL+D+G+VAMSNPLRQSLYTLD+CLNGG++KA+AAV+SL 
Sbjct: 370  AGTLGCQVARMLMSWGVRRITLLDNGRVAMSNPLRQSLYTLDNCLNGGDFKAMAAVESLT 429

Query: 134  QIFPAVEADGIVMAIPMPGHPVPSQDENNVLEDCRRLQDLIDSH 3
            +IFPAVEA+G+VMAIPMPGHPV SQ+E +VLEDCRRL DL+DSH
Sbjct: 430  RIFPAVEAEGVVMAIPMPGHPVSSQEEESVLEDCRRLHDLVDSH 473


>ref|NP_001242518.1| ubiquitin-like modifier-activating enzyme atg7-like [Glycine max]
            gi|256542212|dbj|BAH98059.1| autophagy protein ATG7
            [Glycine max]
          Length = 686

 Score =  729 bits (1883), Expect = 0.0
 Identities = 348/462 (75%), Positives = 404/462 (87%)
 Frame = -1

Query: 1388 ILQFAPFQSAVDEGFWHRLSSLKLNQLGIDESPISVTGFYAPCSHSQVSNHLTLLAESLP 1209
            +LQFAP QS+VDEGFWHRLSSLKLN+LGID+SPI + GFYAPCSHSQVSNHLT+LAESLP
Sbjct: 4    LLQFAPMQSSVDEGFWHRLSSLKLNKLGIDDSPIPLFGFYAPCSHSQVSNHLTVLAESLP 63

Query: 1208 PEPXXXXXXXXXXSGNRNRCSIPGILYNTNTLEGFHALDKQSLLKAEAKKIWEDIHSGKA 1029
             E            GNRNRCS+PGILYNTNT+E FHALDK  LLK EA KIW+DI +GKA
Sbjct: 64   SELSEASLIPEPSRGNRNRCSVPGILYNTNTVESFHALDKSDLLKKEAAKIWDDILTGKA 123

Query: 1028 EEESSVLSRFLLISFADLKKWKFYYWFAFPALVLDPPATITSLKPAAQCFSPEEAESLAA 849
             E+ SVLS FL+ISFADLKKW F YWFAFPAL+LDPPAT+ +LKPA+Q FS  EAESL+A
Sbjct: 124  VEDCSVLSTFLVISFADLKKWTFNYWFAFPALMLDPPATVVNLKPASQWFSAAEAESLSA 183

Query: 848  VCNEWRNSSSTADVPFFLVSIDLNTHASIRHLKDWEACRSDDHEYLFGFYDPCHLPNNPG 669
             CNEWR+S S  DVPFFLV+ID N+ A++R LKDWEAC+S+ H+ LFGFYDPCHLPNNPG
Sbjct: 184  ACNEWRSSKSKTDVPFFLVTIDPNSRATVRLLKDWEACQSNAHKILFGFYDPCHLPNNPG 243

Query: 668  WPLRNFIAFICSRWNLQKIRFFCYREHRGFADMKMSLIGEALISVSQEWKHHKHVPNAVG 489
            WPLRNF+A I +RWNL  ++FFCYRE+RGFADM++SL+GEALI+V Q WK    VP+AVG
Sbjct: 244  WPLRNFLALISARWNLNSVQFFCYRENRGFADMRLSLVGEALITVPQGWK--DTVPSAVG 301

Query: 488  WEVNKGKKISRCITLANSMDPTKLAVSAADLNLKLMRWRALPSLDLNMLSATRCLLLGAG 309
            WE+NKG+K  RCI+LA SMDPT+LA+SAADLNLKLMRWRALPSL+LN LS+ +CLLLGAG
Sbjct: 302  WELNKGRKAPRCISLAQSMDPTRLAISAADLNLKLMRWRALPSLNLNALSSMKCLLLGAG 361

Query: 308  TLGCQVARMLMAWGVRKITLVDSGKVAMSNPLRQSLYTLDDCLNGGEYKALAAVKSLKQI 129
            TLGCQVARMLMAWGVRKITLVD+G+VAMSNPLRQSLYTLDDCLNGGE+KA AAV+SLK+I
Sbjct: 362  TLGCQVARMLMAWGVRKITLVDNGRVAMSNPLRQSLYTLDDCLNGGEFKATAAVESLKRI 421

Query: 128  FPAVEADGIVMAIPMPGHPVPSQDENNVLEDCRRLQDLIDSH 3
            FPAVEA+GIVMAIPMPGHPV SQ++++VL+DC+RL DLID+H
Sbjct: 422  FPAVEAEGIVMAIPMPGHPVQSQEQDSVLDDCKRLCDLIDAH 463


>ref|XP_002305077.1| predicted protein [Populus trichocarpa] gi|222848041|gb|EEE85588.1|
            predicted protein [Populus trichocarpa]
          Length = 715

 Score =  695 bits (1794), Expect = 0.0
 Identities = 332/471 (70%), Positives = 388/471 (82%)
 Frame = -1

Query: 1415 SMAGHENGPILQFAPFQSAVDEGFWHRLSSLKLNQLGIDESPISVTGFYAPCSHSQVSNH 1236
            S  G  +  ILQF PF S  DEGFWHRLSSLKLN+ GID+SPI +TGFYAPCSHSQVSNH
Sbjct: 9    SGCGSSSSTILQFVPFNSLADEGFWHRLSSLKLNKYGIDDSPIPITGFYAPCSHSQVSNH 68

Query: 1235 LTLLAESLPPEPXXXXXXXXXXSGNRNRCSIPGILYNTNTLEGFHALDKQSLLKAEAKKI 1056
            L LLAESLP +            GNRNRC +PG LYNTNTLE FHALDK+SLLK EA KI
Sbjct: 69   LRLLAESLPTDENDQSSMPAISRGNRNRCPVPGTLYNTNTLEAFHALDKKSLLKEEANKI 128

Query: 1055 WEDIHSGKAEEESSVLSRFLLISFADLKKWKFYYWFAFPALVLDPPATITSLKPAAQCFS 876
            WEDIH+G+A E+S+VLSRFLLISFADLKKW F+YWFAFPALVLDPPAT+   K A++ F+
Sbjct: 129  WEDIHNGRAVEDSAVLSRFLLISFADLKKWSFHYWFAFPALVLDPPATLVESKRASEWFT 188

Query: 875  PEEAESLAAVCNEWRNSSSTADVPFFLVSIDLNTHASIRHLKDWEACRSDDHEYLFGFYD 696
             EE +S++  CN+WRNSS TADVPFF +SI  N+HA+IRHLKDWEAC++D+ + LFGFYD
Sbjct: 189  SEEVKSVSVACNDWRNSSLTADVPFFFISIASNSHATIRHLKDWEACQADNQKVLFGFYD 248

Query: 695  PCHLPNNPGWPLRNFIAFICSRWNLQKIRFFCYREHRGFADMKMSLIGEALISVSQEWKH 516
            PCH   +PGWPLRNF+A I SRWNL+ + F C+RE RGF DM+ SL+ EALI+  Q    
Sbjct: 249  PCH-EKDPGWPLRNFLALISSRWNLKSVHFLCFRESRGFMDMESSLVIEALITAPQGLND 307

Query: 515  HKHVPNAVGWEVNKGKKISRCITLANSMDPTKLAVSAADLNLKLMRWRALPSLDLNMLSA 336
             + VPNAVGWE NK K + RCI LA SMDPT+LAVSAADLNLKLMRWRALPSL+L+ LS+
Sbjct: 308  RQLVPNAVGWEKNKNKYVYRCINLATSMDPTRLAVSAADLNLKLMRWRALPSLNLDELSS 367

Query: 335  TRCLLLGAGTLGCQVARMLMAWGVRKITLVDSGKVAMSNPLRQSLYTLDDCLNGGEYKAL 156
             +CLL+GAGTLGCQVARMLMAWGVRKITL+D+G+VAMSNPLRQSLYTLDDCL+GG++KAL
Sbjct: 368  VKCLLIGAGTLGCQVARMLMAWGVRKITLLDNGRVAMSNPLRQSLYTLDDCLDGGDFKAL 427

Query: 155  AAVKSLKQIFPAVEADGIVMAIPMPGHPVPSQDENNVLEDCRRLQDLIDSH 3
            AA KSLK+IFPAVEA G VMAIPMPGHPV +Q+E +V++DC RL DL+DSH
Sbjct: 428  AAAKSLKRIFPAVEAKGEVMAIPMPGHPVTNQEEKSVVDDCSRLYDLVDSH 478