BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00006638
         (1128 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002285122.1| PREDICTED: metal transporter Nramp2-like [Vi...   456   e-126
ref|XP_002894035.1| NRAMP2 [Arabidopsis lyrata subsp. lyrata] gi...   442   e-122
ref|XP_002510129.1| natural resistance-associated macrophage pro...   441   e-121
ref|XP_003636821.1| Natural resistance-associated macrophage pro...   440   e-121
gb|AAD41078.1|AF141204_1 Nramp2 [Arabidopsis thaliana]                437   e-120

>ref|XP_002285122.1| PREDICTED: metal transporter Nramp2-like [Vitis vinifera]
          Length = 536

 Score =  456 bits (1174), Expect = e-126
 Identities = 234/300 (78%), Positives = 260/300 (86%), Gaps = 6/300 (2%)
 Frame = +2

Query: 245  PENTTAPPPIPN--NEESHRLLSTKPPPSIAGDYGDE-VAYESQDKILIVDFD---VEST 406
            PE +   PP  +  +EE++RLL +K P S++    +E VAYES++KI IVD +   +ES 
Sbjct: 4    PEPSQQEPPNGDGKDEEANRLLPSKSPSSLSPSSDEEEVAYESREKISIVDLEGETLESL 63

Query: 407  DELVPPQFSWKKLWLFTGPGFLMSIAFLDPGNLEGDLQAGAIAGYSLLWLLMWATGMGLL 586
            D    P FSWKKLWLFTGPGFLMSIAFLDPGNLEGDLQAGAIAGYSLLWLLMWAT MGLL
Sbjct: 64   DASATPPFSWKKLWLFTGPGFLMSIAFLDPGNLEGDLQAGAIAGYSLLWLLMWATVMGLL 123

Query: 587  IQLLSARVGVVTGKHLAELCREEYPKWAGLLLWFMAELALIGADIQEVIGSAIAIQILSH 766
            IQLLSARVGV TG+HLAELCREEYP WAG LLWFMAELALIGADIQEVIGSAIAI+ILS 
Sbjct: 124  IQLLSARVGVATGRHLAELCREEYPTWAGRLLWFMAELALIGADIQEVIGSAIAIKILSR 183

Query: 767  GVLPLWAGVLITASDCFIFLLLENYGVRKLEAVFAVLISTMGLSFAWMFADTEPSGKELL 946
            G LPLWAGV+ITASDCFIFL LENYGVRKLEAVFAV I+TMGLSFAWMF D +PSG+ELL
Sbjct: 184  GFLPLWAGVVITASDCFIFLFLENYGVRKLEAVFAVFIATMGLSFAWMFGDAKPSGRELL 243

Query: 947  IGLLVPKLSSRTIRQAVGVVGCVIMPHNVFLHSALVQSRNVDPSKKGQVQEAVKYYNIES 1126
            IG+LVPKLSS+TIRQAVGVVGCVIMPHNVFLHSALVQSR +DP +KG+VQEA+ YY+IES
Sbjct: 244  IGVLVPKLSSKTIRQAVGVVGCVIMPHNVFLHSALVQSRKIDPHRKGRVQEALNYYSIES 303


>ref|XP_002894035.1| NRAMP2 [Arabidopsis lyrata subsp. lyrata] gi|297339877|gb|EFH70294.1|
            NRAMP2 [Arabidopsis lyrata subsp. lyrata]
          Length = 532

 Score =  442 bits (1136), Expect = e-122
 Identities = 224/286 (78%), Positives = 250/286 (87%), Gaps = 5/286 (1%)
 Frame = +2

Query: 284  EESHRLLSTKPPPSI-AGDYGDEVAYESQDKILIVDFDVESTDELV----PPQFSWKKLW 448
            EE++RLL    P S+ + D   E A+ES +KILIVDF  ES D+      PP FSW+KLW
Sbjct: 14   EEANRLLPPPHPQSLPSSDSESEAAFESNEKILIVDF--ESPDDPTTGDSPPPFSWRKLW 71

Query: 449  LFTGPGFLMSIAFLDPGNLEGDLQAGAIAGYSLLWLLMWATGMGLLIQLLSARVGVVTGK 628
            LFTGPGFLMSIAFLDPGNLEGDLQAGAIAGYSLLWLLMWAT MGLLIQ+LSARVGV TG+
Sbjct: 72   LFTGPGFLMSIAFLDPGNLEGDLQAGAIAGYSLLWLLMWATAMGLLIQMLSARVGVATGR 131

Query: 629  HLAELCREEYPKWAGLLLWFMAELALIGADIQEVIGSAIAIQILSHGVLPLWAGVLITAS 808
            HLAELCR+EYP WA  +LW MAELALIGADIQEVIGSAIAIQILS GVLPLWAGV+ITAS
Sbjct: 132  HLAELCRDEYPTWARYVLWSMAELALIGADIQEVIGSAIAIQILSRGVLPLWAGVVITAS 191

Query: 809  DCFIFLLLENYGVRKLEAVFAVLISTMGLSFAWMFADTEPSGKELLIGLLVPKLSSRTIR 988
            DCF+FL LENYGVRKLEAVFAVLI+TMGLSFAWMF +T+PSGKEL+IG+L+P+LSS+TIR
Sbjct: 192  DCFLFLFLENYGVRKLEAVFAVLIATMGLSFAWMFGETKPSGKELMIGILLPRLSSKTIR 251

Query: 989  QAVGVVGCVIMPHNVFLHSALVQSRNVDPSKKGQVQEAVKYYNIES 1126
            QAVGVVGCVIMPHNVFLHSALVQSR +DP +K +VQEA+ YY IES
Sbjct: 252  QAVGVVGCVIMPHNVFLHSALVQSRKIDPKRKSRVQEALNYYLIES 297


>ref|XP_002510129.1| natural resistance-associated macrophage protein, putative [Ricinus
            communis] gi|223550830|gb|EEF52316.1| natural
            resistance-associated macrophage protein, putative
            [Ricinus communis]
          Length = 462

 Score =  441 bits (1134), Expect = e-121
 Identities = 221/284 (77%), Positives = 247/284 (86%), Gaps = 3/284 (1%)
 Frame = +2

Query: 284  EESHRLLSTKPPPSIAGDYGDEVAYESQDKILIVDFDVES---TDELVPPQFSWKKLWLF 454
            +ES RLLST     I     DEVA+E++DKILIVD D +     D+  PP FSWKKLWLF
Sbjct: 18   DESTRLLSTSAESPINPTLDDEVAFEARDKILIVDLDSQDGIDADDYTPP-FSWKKLWLF 76

Query: 455  TGPGFLMSIAFLDPGNLEGDLQAGAIAGYSLLWLLMWATGMGLLIQLLSARVGVVTGKHL 634
            TGPGFLMSIAFLDPGNLEGDLQAGAIAGYSLLWLLMWAT MGLLIQ+LSARVGV TG+HL
Sbjct: 77   TGPGFLMSIAFLDPGNLEGDLQAGAIAGYSLLWLLMWATVMGLLIQMLSARVGVATGRHL 136

Query: 635  AELCREEYPKWAGLLLWFMAELALIGADIQEVIGSAIAIQILSHGVLPLWAGVLITASDC 814
            AELCREEYP WA L+LWFMAE+ALIGADIQEVIGSAIAI ILS+G LPLWAGV+ITA DC
Sbjct: 137  AELCREEYPNWARLILWFMAEVALIGADIQEVIGSAIAIHILSNGFLPLWAGVVITALDC 196

Query: 815  FIFLLLENYGVRKLEAVFAVLISTMGLSFAWMFADTEPSGKELLIGLLVPKLSSRTIRQA 994
            F+FL LENYGVRKLEA FA+LI+TM +SFAWMF DT+PSGKELL+G+L+P+LSS+TIRQA
Sbjct: 197  FMFLFLENYGVRKLEAAFAILIATMAISFAWMFGDTKPSGKELLMGILIPRLSSKTIRQA 256

Query: 995  VGVVGCVIMPHNVFLHSALVQSRNVDPSKKGQVQEAVKYYNIES 1126
            VGVVGCVIMPHNVFLHSALVQSR ++  KKG+VQEA+ YY IES
Sbjct: 257  VGVVGCVIMPHNVFLHSALVQSRKIETQKKGRVQEALNYYLIES 300


>ref|XP_003636821.1| Natural resistance-associated macrophage protein [Medicago
            truncatula] gi|358348881|ref|XP_003638470.1| Natural
            resistance-associated macrophage protein [Medicago
            truncatula] gi|355502756|gb|AES83959.1| Natural
            resistance-associated macrophage protein [Medicago
            truncatula] gi|355504405|gb|AES85608.1| Natural
            resistance-associated macrophage protein [Medicago
            truncatula]
          Length = 528

 Score =  440 bits (1132), Expect = e-121
 Identities = 217/285 (76%), Positives = 244/285 (85%)
 Frame = +2

Query: 272  IPNNEESHRLLSTKPPPSIAGDYGDEVAYESQDKILIVDFDVESTDELVPPQFSWKKLWL 451
            +   +E +RLL        + +  +E AYES +KI++VDF+  + D+   P FSWKKLW+
Sbjct: 14   VKEEDEGNRLLEVNVEGESSSE--EEAAYESGEKIVVVDFEFGTVDDSTVPPFSWKKLWM 71

Query: 452  FTGPGFLMSIAFLDPGNLEGDLQAGAIAGYSLLWLLMWATGMGLLIQLLSARVGVVTGKH 631
            FTGPGFLMSIAFLDPGNLEGDLQAGAIAGYSLLWLLMWAT MGLLIQ LSARVGV TG+H
Sbjct: 72   FTGPGFLMSIAFLDPGNLEGDLQAGAIAGYSLLWLLMWATLMGLLIQSLSARVGVATGRH 131

Query: 632  LAELCREEYPKWAGLLLWFMAELALIGADIQEVIGSAIAIQILSHGVLPLWAGVLITASD 811
            LAELC+EEYP WA  +LWFMAELALIGADIQEVIGSAIAIQILS GVLPLWAGVLITASD
Sbjct: 132  LAELCKEEYPNWARYVLWFMAELALIGADIQEVIGSAIAIQILSRGVLPLWAGVLITASD 191

Query: 812  CFIFLLLENYGVRKLEAVFAVLISTMGLSFAWMFADTEPSGKELLIGLLVPKLSSRTIRQ 991
            CF FL LENYGVRKLEA FAVLI+TM LSFAWMF D +PSGKEL+ G+L+P+LSS+TI Q
Sbjct: 192  CFFFLFLENYGVRKLEAAFAVLIATMALSFAWMFGDAQPSGKELVRGILIPRLSSKTIHQ 251

Query: 992  AVGVVGCVIMPHNVFLHSALVQSRNVDPSKKGQVQEAVKYYNIES 1126
            AVGVVGCVIMPHNVFLHSALVQSR +DP+KKG+VQEA+ YYNIES
Sbjct: 252  AVGVVGCVIMPHNVFLHSALVQSRKIDPNKKGRVQEALNYYNIES 296


>gb|AAD41078.1|AF141204_1 Nramp2 [Arabidopsis thaliana]
          Length = 530

 Score =  437 bits (1124), Expect = e-120
 Identities = 220/285 (77%), Positives = 245/285 (85%), Gaps = 4/285 (1%)
 Frame = +2

Query: 284  EESHRLLSTKPPPSI--AGDYGDEVAYESQDKILIVDFDV--ESTDELVPPQFSWKKLWL 451
            EE  RLL   PP     + D   E A+E+ +KILIVDF+   + T    PP FSW+KLWL
Sbjct: 11   EEEDRLLPPPPPSQSLPSTDSESEAAFETNEKILIVDFESPDDPTTGDTPPPFSWRKLWL 70

Query: 452  FTGPGFLMSIAFLDPGNLEGDLQAGAIAGYSLLWLLMWATGMGLLIQLLSARVGVVTGKH 631
            FTGPGFLMSIAFLDPGNLEGDLQAGAIAGYSLLWLLMWAT MGLLIQ+LSARVGV TG+H
Sbjct: 71   FTGPGFLMSIAFLDPGNLEGDLQAGAIAGYSLLWLLMWATAMGLLIQMLSARVGVATGRH 130

Query: 632  LAELCREEYPKWAGLLLWFMAELALIGADIQEVIGSAIAIQILSHGVLPLWAGVLITASD 811
            LAELCR+EYP WA  +LW MAELALIGADIQEVIGSAIAIQILS G LPLWAGV+ITASD
Sbjct: 131  LAELCRDEYPTWARYVLWSMAELALIGADIQEVIGSAIAIQILSRGFLPLWAGVVITASD 190

Query: 812  CFIFLLLENYGVRKLEAVFAVLISTMGLSFAWMFADTEPSGKELLIGLLVPKLSSRTIRQ 991
            CF+FL LENYGVRKLEAVFAVLI+TMGLSFAWMF +T+PSGKEL+IG+L+P+LSS+TIRQ
Sbjct: 191  CFLFLFLENYGVRKLEAVFAVLIATMGLSFAWMFGETKPSGKELMIGILLPRLSSKTIRQ 250

Query: 992  AVGVVGCVIMPHNVFLHSALVQSRNVDPSKKGQVQEAVKYYNIES 1126
            AVGVVGCVIMPHNVFLHSALVQSR +DP +K +VQEA+ YY IES
Sbjct: 251  AVGVVGCVIMPHNVFLHSALVQSRKIDPKRKSRVQEALNYYLIES 295