BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00006593
         (1597 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002516244.1| polygalacturonase, putative [Ricinus communi...   801   0.0  
ref|XP_002277239.1| PREDICTED: probable polygalacturonase [Vitis...   797   0.0  
emb|CAN69988.1| hypothetical protein VITISV_024399 [Vitis vinifera]   784   0.0  
ref|XP_003551102.1| PREDICTED: probable polygalacturonase-like i...   781   0.0  
ref|XP_002326133.1| predicted protein [Populus trichocarpa] gi|2...   778   0.0  

>ref|XP_002516244.1| polygalacturonase, putative [Ricinus communis]
            gi|223544730|gb|EEF46246.1| polygalacturonase, putative
            [Ricinus communis]
          Length = 494

 Score =  801 bits (2069), Expect = 0.0
 Identities = 373/454 (82%), Positives = 427/454 (94%), Gaps = 1/454 (0%)
 Frame = -2

Query: 1359 RPDIRRRVTTFFSSHRTLFTLLWILAFSSVFIWQRNIV-DGLSVFRRPVMMREMPKLRPV 1183
            R +++R V TFFSSH+TLFT+LWI AF+SVF+WQRN+V DG +VF +   MR MPKLRPV
Sbjct: 15   RLELKRWVPTFFSSHKTLFTVLWIAAFASVFVWQRNVVGDGFAVFFKASPMRPMPKLRPV 74

Query: 1182 AFNLTEFGGVGDGISLNTEAFERAVFAISKLGKKGGGQLNVPAGKWLTAPFNLTSHMTLF 1003
            AFNLT+FGGVGDG++LNTEAFERAV AISKLGKKGGGQLNVP G+W+TAPFNLTSHMTLF
Sbjct: 75   AFNLTDFGGVGDGVTLNTEAFERAVLAISKLGKKGGGQLNVPPGRWVTAPFNLTSHMTLF 134

Query: 1002 LAEDAVILGIDDEKYWPLMPPLPSYGSGREHPGPRYGSLIHGQNLRDVVITGHNGTINGQ 823
            LAED++ILGI+DEKYWPLMPPLPSYG GREHPGPRYGSLIHGQNL+DVVITGHNGTINGQ
Sbjct: 135  LAEDSIILGIEDEKYWPLMPPLPSYGYGREHPGPRYGSLIHGQNLKDVVITGHNGTINGQ 194

Query: 822  GQSWWKKYRQKLLNYTRGPLVQIMWSSDILISNITLRDSPFWTLHPYDCKNITLRNLTIL 643
            GQ+WWKKYRQKLLN+TRGPLVQIMWSSDILI+NITLRDSPFWTLHPYDCKN+T+RN+TIL
Sbjct: 195  GQTWWKKYRQKLLNHTRGPLVQIMWSSDILITNITLRDSPFWTLHPYDCKNVTIRNVTIL 254

Query: 642  APIFQAPNTDGIDPDSCEDMLIEDSYISVGDDAIAIKSGWDQYGISYGRPSKNIHIRNLV 463
            AP+ +APNTDGIDPDSCEDM+IED YISVGDD IAIKSGWDQYGI+Y RPS NI IRNLV
Sbjct: 255  APVLEAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAYRRPSTNILIRNLV 314

Query: 462  VQSMVSAGISIGSEMSGGVSNITVENILIWNSRRAVRIKTAAGRGGYVQNITYRNLTFEN 283
            V+SMVSAGISIGSEMSGGVSNITVEN+L+W+SRRAVRIKTA GRGGYV++ITYRNLTF+N
Sbjct: 315  VRSMVSAGISIGSEMSGGVSNITVENLLVWSSRRAVRIKTAPGRGGYVRHITYRNLTFDN 374

Query: 282  VRVGIVIKTDYNEHPNGEFDPTAIPILEDISYTSIHGEGVRVPVRIHGSSDIPVKNVTFR 103
            VRVGIVIKTDYNEHP+  +DP A+P+L DIS+T +HG+GVRVPVRIHGS +IPV+NVTF+
Sbjct: 375  VRVGIVIKTDYNEHPDEGYDPKAVPVLRDISFTGVHGQGVRVPVRIHGSEEIPVRNVTFQ 434

Query: 102  DMSVGITYKKQHIFQCAYVEGQVIGSVYPAPCEN 1
            DMSVG+TYKK+HIFQCA+V+G+VIG+++PAPCEN
Sbjct: 435  DMSVGLTYKKKHIFQCAFVQGRVIGTIFPAPCEN 468


>ref|XP_002277239.1| PREDICTED: probable polygalacturonase [Vitis vinifera]
            gi|297738532|emb|CBI27777.3| unnamed protein product
            [Vitis vinifera]
          Length = 493

 Score =  797 bits (2058), Expect = 0.0
 Identities = 372/453 (82%), Positives = 419/453 (92%)
 Frame = -2

Query: 1359 RPDIRRRVTTFFSSHRTLFTLLWILAFSSVFIWQRNIVDGLSVFRRPVMMREMPKLRPVA 1180
            RPD++R +  F SSHRTLFT+LWI AF+SVF+WQRNIV+GL  FRR    R +P+LRP  
Sbjct: 16   RPDLKRWIPAFLSSHRTLFTILWIAAFASVFVWQRNIVEGLLTFRR-APARPLPRLRPAV 74

Query: 1179 FNLTEFGGVGDGISLNTEAFERAVFAISKLGKKGGGQLNVPAGKWLTAPFNLTSHMTLFL 1000
            +NLT+FGGVGD +++NTEAFERA+ AISKLGKKGGGQLNVPAG WLTAPFNLTSHMTLFL
Sbjct: 75   YNLTDFGGVGDSVTVNTEAFERAISAISKLGKKGGGQLNVPAGNWLTAPFNLTSHMTLFL 134

Query: 999  AEDAVILGIDDEKYWPLMPPLPSYGSGREHPGPRYGSLIHGQNLRDVVITGHNGTINGQG 820
             +DAVILGI DEKYWPLMPPLPSYG GREH G RYGSLIHGQNL+DVVITGHNGTINGQG
Sbjct: 135  DQDAVILGIQDEKYWPLMPPLPSYGYGREHRGARYGSLIHGQNLKDVVITGHNGTINGQG 194

Query: 819  QSWWKKYRQKLLNYTRGPLVQIMWSSDILISNITLRDSPFWTLHPYDCKNITLRNLTILA 640
            Q+WWKKYRQKLLN+TRGPLVQIMWSSDI+ISNITLRDSPFWTLHPYDCKN+T++N+TILA
Sbjct: 195  QTWWKKYRQKLLNHTRGPLVQIMWSSDIVISNITLRDSPFWTLHPYDCKNVTIKNVTILA 254

Query: 639  PIFQAPNTDGIDPDSCEDMLIEDSYISVGDDAIAIKSGWDQYGISYGRPSKNIHIRNLVV 460
            PIF+APNTDGIDPDSCEDM+IED YISVGDD IAIKSGWDQYG++YGRPS NI IRNLV+
Sbjct: 255  PIFEAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGVAYGRPSVNILIRNLVI 314

Query: 459  QSMVSAGISIGSEMSGGVSNITVENILIWNSRRAVRIKTAAGRGGYVQNITYRNLTFENV 280
            +SMVSAG+SIGSEMSGGVSN+TVEN+L+WNSRRAVRIKT+ GRGGYVQ+I YRNLTF+NV
Sbjct: 315  RSMVSAGVSIGSEMSGGVSNVTVENLLVWNSRRAVRIKTSPGRGGYVQHINYRNLTFDNV 374

Query: 279  RVGIVIKTDYNEHPNGEFDPTAIPILEDISYTSIHGEGVRVPVRIHGSSDIPVKNVTFRD 100
            RVGIVIKTDYNEHP+  FDP A+PILEDIS+T +HG+GVRVPVRIHGS +IPVK VTFRD
Sbjct: 375  RVGIVIKTDYNEHPDDGFDPKALPILEDISFTGVHGQGVRVPVRIHGSEEIPVKKVTFRD 434

Query: 99   MSVGITYKKQHIFQCAYVEGQVIGSVYPAPCEN 1
            MSVGITYKK+HIFQCAYVEG+VIG+V+PAPCEN
Sbjct: 435  MSVGITYKKKHIFQCAYVEGRVIGTVFPAPCEN 467


>emb|CAN69988.1| hypothetical protein VITISV_024399 [Vitis vinifera]
          Length = 509

 Score =  784 bits (2024), Expect = 0.0
 Identities = 370/469 (78%), Positives = 419/469 (89%), Gaps = 16/469 (3%)
 Frame = -2

Query: 1359 RPDIRRRVTTFFSSHRTLFTLLWILAFSSVFIWQRNIVDGLSVFRRPVMMREMPKLRPVA 1180
            RPD++R +  F SSHRTLFT+LWI AF+SVF+WQRNIV+GL  FRR    R +P+LRP  
Sbjct: 16   RPDLKRWIPAFLSSHRTLFTILWIAAFASVFVWQRNIVEGLLTFRR-APARPLPRLRPAV 74

Query: 1179 FNLTEFGGVGDGISLNTEAFERAVFAISKLGKKGGGQLNVPAGKWLTAPFNLTSHMTLFL 1000
            +NLT+FGGVGD +++NTEAFERA+ AISKLGKKGGGQLNVPAG WLTAPFNLTSHMTLFL
Sbjct: 75   YNLTDFGGVGDXVTVNTEAFERAISAISKLGKKGGGQLNVPAGNWLTAPFNLTSHMTLFL 134

Query: 999  AEDAVILGIDDEKYWPLMPPLPSYGSGREHPGPRYGSLIHGQNLRDVVITGHNGTINGQG 820
             +DAVILGI DEKYWPLMPPLPSYG GREH G RYGSLIHGQNL+DVV+TGHNGTINGQG
Sbjct: 135  DQDAVILGIQDEKYWPLMPPLPSYGYGREHRGARYGSLIHGQNLKDVVVTGHNGTINGQG 194

Query: 819  QSWWKKYRQKLLNYTRGPLVQIMWSSDILISNITLRDSPFWTLHPYDCKNITLRNLTILA 640
            Q+WWKKYRQKLLN+TRGPLVQIMWSSDI+ISNITLRDSPFWTLHPYDCKN+T++N+TILA
Sbjct: 195  QTWWKKYRQKLLNHTRGPLVQIMWSSDIVISNITLRDSPFWTLHPYDCKNVTIKNVTILA 254

Query: 639  PIFQAPNTDGID----------------PDSCEDMLIEDSYISVGDDAIAIKSGWDQYGI 508
            PIF+APNTDGID                P+SCEDM+IED YISVGDD IAIKSGWDQYG+
Sbjct: 255  PIFEAPNTDGIDPGKVTCTLYYFSDCICPNSCEDMVIEDCYISVGDDGIAIKSGWDQYGV 314

Query: 507  SYGRPSKNIHIRNLVVQSMVSAGISIGSEMSGGVSNITVENILIWNSRRAVRIKTAAGRG 328
            +YGRPS NI IRNLV++SMVSAG+SIGSEMSGGVSN+TVEN+L+WNSRRAVRIKT+ GRG
Sbjct: 315  AYGRPSVNILIRNLVIRSMVSAGVSIGSEMSGGVSNVTVENLLVWNSRRAVRIKTSPGRG 374

Query: 327  GYVQNITYRNLTFENVRVGIVIKTDYNEHPNGEFDPTAIPILEDISYTSIHGEGVRVPVR 148
            GYVQ+I YRNLTF+NVRVGIVIKTDYNEHP+  FDP A+PILEDIS+T +HG+GVRVPVR
Sbjct: 375  GYVQHINYRNLTFDNVRVGIVIKTDYNEHPDDGFDPKALPILEDISFTGVHGQGVRVPVR 434

Query: 147  IHGSSDIPVKNVTFRDMSVGITYKKQHIFQCAYVEGQVIGSVYPAPCEN 1
            IHGS +IPVK VTFRDMSVGITYKK+HIFQCAYVEG+VIG+V+PAPCEN
Sbjct: 435  IHGSEEIPVKKVTFRDMSVGITYKKKHIFQCAYVEGRVIGTVFPAPCEN 483


>ref|XP_003551102.1| PREDICTED: probable polygalacturonase-like isoform 1 [Glycine max]
          Length = 492

 Score =  781 bits (2017), Expect = 0.0
 Identities = 372/467 (79%), Positives = 421/467 (90%), Gaps = 4/467 (0%)
 Frame = -2

Query: 1389 MMVENLT----PPTRPDIRRRVTTFFSSHRTLFTLLWILAFSSVFIWQRNIVDGLSVFRR 1222
            MMVE  T       R D+RR V  FF+SH+TL TLLWI AF+SVF+WQRNIV G  V+  
Sbjct: 1    MMVETSTLGHFHHQRLDLRRWVPAFFTSHKTLLTLLWIAAFASVFLWQRNIVGGFLVYGG 60

Query: 1221 PVMMREMPKLRPVAFNLTEFGGVGDGISLNTEAFERAVFAISKLGKKGGGQLNVPAGKWL 1042
             +  R MP LRP+AFNLT+FGGVGDG++LNTEAFERAV A+SK GKKGG QLNVP G+WL
Sbjct: 61   -IPGRPMPMLRPMAFNLTDFGGVGDGVTLNTEAFERAVSAVSKFGKKGGAQLNVPPGRWL 119

Query: 1041 TAPFNLTSHMTLFLAEDAVILGIDDEKYWPLMPPLPSYGSGREHPGPRYGSLIHGQNLRD 862
            TAPFNLTSHMTLFLAEDAVILGIDDEKYWPLMPPLPSYG GREHPGPRYGSLIHGQ+L+D
Sbjct: 120  TAPFNLTSHMTLFLAEDAVILGIDDEKYWPLMPPLPSYGYGREHPGPRYGSLIHGQHLKD 179

Query: 861  VVITGHNGTINGQGQSWWKKYRQKLLNYTRGPLVQIMWSSDILISNITLRDSPFWTLHPY 682
            VVITGHNGTINGQGQSWWKKYRQK LN+TRGPLVQIM+SSDI+I+NITLRDSPFWT+HPY
Sbjct: 180  VVITGHNGTINGQGQSWWKKYRQKRLNHTRGPLVQIMFSSDIVITNITLRDSPFWTIHPY 239

Query: 681  DCKNITLRNLTILAPIFQAPNTDGIDPDSCEDMLIEDSYISVGDDAIAIKSGWDQYGISY 502
            DCKNIT++ +TILAP+F APNTDGIDPDSCEDMLIED YISVGDDAIA+KSGWDQYGI Y
Sbjct: 240  DCKNITIKGVTILAPVFGAPNTDGIDPDSCEDMLIEDCYISVGDDAIAVKSGWDQYGIDY 299

Query: 501  GRPSKNIHIRNLVVQSMVSAGISIGSEMSGGVSNITVENILIWNSRRAVRIKTAAGRGGY 322
            GRPS NI IRNLVV+SMVSAGISIGSEMSGGVSN+TVEN+LIW+SRR VRIKTA GRG Y
Sbjct: 300  GRPSMNIMIRNLVVRSMVSAGISIGSEMSGGVSNVTVENLLIWDSRRGVRIKTAPGRGAY 359

Query: 321  VQNITYRNLTFENVRVGIVIKTDYNEHPNGEFDPTAIPILEDISYTSIHGEGVRVPVRIH 142
            V+ ITYRN+TFENVRVGIV+KTDYNEHP+  +DP A+PIL DIS+T++HG+GVRVPVRIH
Sbjct: 360  VRQITYRNITFENVRVGIVMKTDYNEHPDDGYDPLALPILRDISFTTVHGQGVRVPVRIH 419

Query: 141  GSSDIPVKNVTFRDMSVGITYKKQHIFQCAYVEGQVIGSVYPAPCEN 1
            GS +IPV+NVTF+DMSVG+TYKK+HIFQCA+V+G+VIG++YPAPCEN
Sbjct: 420  GSEEIPVRNVTFKDMSVGLTYKKKHIFQCAFVQGRVIGTIYPAPCEN 466


>ref|XP_002326133.1| predicted protein [Populus trichocarpa] gi|222833326|gb|EEE71803.1|
            predicted protein [Populus trichocarpa]
          Length = 495

 Score =  778 bits (2008), Expect = 0.0
 Identities = 374/470 (79%), Positives = 424/470 (90%), Gaps = 8/470 (1%)
 Frame = -2

Query: 1386 MVENLTPPT-------RPDIRRRVTTFFSSHRTLFTLLWILAFSSVFIWQRNIVD-GLSV 1231
            MVE + PP        R +++R +  F SSH+TLF LLWI AF+SVF+W+RN V  GL+V
Sbjct: 1    MVETIAPPPSSRFNYKRLELKRCLPAFLSSHKTLFALLWIAAFASVFLWKRNPVGAGLAV 60

Query: 1230 FRRPVMMREMPKLRPVAFNLTEFGGVGDGISLNTEAFERAVFAISKLGKKGGGQLNVPAG 1051
            F R  + R MP+LRPVAFNLT+FG VGDG ++NTEAFERAV AISKL K+GGGQLNVP G
Sbjct: 61   FWRGPL-RPMPQLRPVAFNLTDFGAVGDGATVNTEAFERAVSAISKLSKRGGGQLNVPPG 119

Query: 1050 KWLTAPFNLTSHMTLFLAEDAVILGIDDEKYWPLMPPLPSYGSGREHPGPRYGSLIHGQN 871
            +WLTAPFNLTSHMTLFLAE AVILGI DEKYWPLMP LPSYG GREHPGPRYGSLIHGQN
Sbjct: 120  RWLTAPFNLTSHMTLFLAEGAVILGIQDEKYWPLMPALPSYGYGREHPGPRYGSLIHGQN 179

Query: 870  LRDVVITGHNGTINGQGQSWWKKYRQKLLNYTRGPLVQIMWSSDILISNITLRDSPFWTL 691
            LRDVVITGHNGTI+GQGQ+WWKKYRQKLLN+TRGPLVQIMWSSDI+ +NITLRDSPFWTL
Sbjct: 180  LRDVVITGHNGTIDGQGQTWWKKYRQKLLNHTRGPLVQIMWSSDIVFTNITLRDSPFWTL 239

Query: 690  HPYDCKNITLRNLTILAPIFQAPNTDGIDPDSCEDMLIEDSYISVGDDAIAIKSGWDQYG 511
            HPYDCKN+T+RN+TILAPIF+APNTDGIDPDSCEDM+IED YISVGDDAIAIKSGWDQYG
Sbjct: 240  HPYDCKNVTIRNVTILAPIFEAPNTDGIDPDSCEDMVIEDCYISVGDDAIAIKSGWDQYG 299

Query: 510  ISYGRPSKNIHIRNLVVQSMVSAGISIGSEMSGGVSNITVENILIWNSRRAVRIKTAAGR 331
            I+YGRPS NI IRNLVV+SMVSAGISIGSEMSGGVSN+TVEN+L+W+SRRAVRIKTA GR
Sbjct: 300  IAYGRPSTNILIRNLVVRSMVSAGISIGSEMSGGVSNVTVENVLVWSSRRAVRIKTAPGR 359

Query: 330  GGYVQNITYRNLTFENVRVGIVIKTDYNEHPNGEFDPTAIPILEDISYTSIHGEGVRVPV 151
            GGYV++ITYRNLTF+NVRVGIVIKTDYNEHP+  +DP A+PIL DIS+T IHG+GVRVPV
Sbjct: 360  GGYVRHITYRNLTFDNVRVGIVIKTDYNEHPDEGYDPKAVPILGDISFTGIHGQGVRVPV 419

Query: 150  RIHGSSDIPVKNVTFRDMSVGITYKKQHIFQCAYVEGQVIGSVYPAPCEN 1
            RIHGS +IPV+NVTF DMSVG+TYKK+HIFQCA+V+G+VIG+V+PAPCEN
Sbjct: 420  RIHGSQEIPVRNVTFWDMSVGLTYKKKHIFQCAFVQGRVIGTVFPAPCEN 469