BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00006479
         (1411 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AEF32084.1| ent-kaurene oxidase [Castanea mollissima]              526   e-147
ref|XP_002510288.1| ent-kaurene oxidase, putative [Ricinus commu...   525   e-147
ref|XP_002282197.1| PREDICTED: ent-kaurene oxidase, chloroplasti...   523   e-146
gb|AFD54196.1| ent-kaurene oxidase [Vitis vinifera]                   520   e-145
gb|ACQ99375.1| ent-kaurene oxidase 1 [Coffea arabica]                 506   e-141

>gb|AEF32084.1| ent-kaurene oxidase [Castanea mollissima]
          Length = 514

 Score =  526 bits (1354), Expect = e-147
 Identities = 257/464 (55%), Positives = 345/464 (74%)
 Frame = -1

Query: 1393 VVSLSTSWQ*YQMDVFAYLASTPIITLVVCGFSLWFLKRFVKHNKKSPPNLRPLPVVAGG 1214
            + S++   Q +Q   FA   +   ++L++  F   F++ F    K     L P+PVV G 
Sbjct: 1    MASITHFLQDFQATPFATAFAVGGVSLLIFFF---FIRGFHSTKKNEYYKLPPVPVVPG- 56

Query: 1213 LPIVGNLLQLKEKKPHKIFLKWVETYGPVYSIRTGYNTIVVINSIDVAKEAMVTKFDSIS 1034
            LP+VGNLLQLKEKKP+K FL+W E +GP+YSIRTG +T+VV+NS  VAKEAMVT+F SIS
Sbjct: 57   LPVVGNLLQLKEKKPYKTFLRWAEIHGPIYSIRTGASTMVVVNSTHVAKEAMVTRFSSIS 116

Query: 1033 TKKLSTAVKILADDKSMVATSDYDEFHKIAKRQLLTHLLGPIAQKRNRIDRDIVVENTLN 854
            T+KLS A+++L  +KSMVATSDY+EFHK+ K+ +L  LLG  AQKR+RI RD ++EN LN
Sbjct: 117  TRKLSKALELLTSNKSMVATSDYNEFHKMVKKYILAELLGANAQKRHRIHRDTLIENVLN 176

Query: 853  KVQASVKKSPLEPINFRKIFELELFSLGMKHALGLDVDSIYVDELGITMSKEDMFQCIVI 674
            K+ A  K SPL+ +NFRKIFE ELF L MK ALG DVDS++V+ELG T+S+E+++  +V 
Sbjct: 177  KLHAHTKNSPLQAVNFRKIFESELFGLAMKQALGYDVDSLFVEELGTTLSREEIYNVLVS 236

Query: 673  DPMAGSIEVDWRDFFPYLKWIPNRSLEKKVEQMYIRKTAVIKALIKKTKTQSASKEGVPC 494
            D + G+IEVDWRDFFPYLKWIPN+S E K++++  R+ AV+ +++K+ K   AS +G  C
Sbjct: 237  DMLKGAIEVDWRDFFPYLKWIPNKSFEMKIQRLASRRQAVMNSIVKEQKKSIASGKGENC 296

Query: 493  XXXXXXXXXXXXXXXKQIHMLMWEVLIATADTTLVATEWAMYEIARDSKRQQRLYEEISR 314
                           KQI +L WE +I TADTT+V TEWAMYE+A++ K+Q RLY EI  
Sbjct: 297  -YLNYLLSEAKTLTEKQISILAWETIIETADTTVVTTEWAMYELAKNPKQQDRLYNEIQN 355

Query: 313  VCGSNRITEEHLHQLPYLYAIFQETIRVYSPFPVTPLRYVHEDIELGGYFVPEGTEIAIN 134
            VCG+++ITEEHL +LPYL A+F ET+R YSP P+ PLRY HED +LGGY+VP GTEIA+N
Sbjct: 356  VCGTDKITEEHLSKLPYLSAVFHETLRKYSPSPLVPLRYAHEDTQLGGYYVPAGTEIAVN 415

Query: 133  IYGCNMDKSIWKNPEEWKPERFMEEESDTMELHRSLAFGGGKRV 2
            IYGCNMDK+ W+ PEEWKPERF++E+ D M+++++++FG GKRV
Sbjct: 416  IYGCNMDKNQWETPEEWKPERFLDEKYDPMDMYKTMSFGSGKRV 459


>ref|XP_002510288.1| ent-kaurene oxidase, putative [Ricinus communis]
            gi|223550989|gb|EEF52475.1| ent-kaurene oxidase, putative
            [Ricinus communis]
          Length = 513

 Score =  525 bits (1353), Expect = e-147
 Identities = 251/432 (58%), Positives = 328/432 (75%)
 Frame = -1

Query: 1300 FSLWFLKRFVKHNKKSPPNLRPLPVVAGGLPIVGNLLQLKEKKPHKIFLKWVETYGPVYS 1121
            FS++ +K+ + + KK    L P+P V G  P++GNLLQLKEKKPHK F++W ETYGP+YS
Sbjct: 28   FSMFVMKKLISNQKKRHARLPPVPEVPGW-PVIGNLLQLKEKKPHKTFMRWAETYGPIYS 86

Query: 1120 IRTGYNTIVVINSIDVAKEAMVTKFDSISTKKLSTAVKILADDKSMVATSDYDEFHKIAK 941
            IRTG +TI+V+NS DVAKEAMVT++ SIST+KLS A+ IL  DK MVATSDYDEFHK+ K
Sbjct: 87   IRTGASTIIVLNSTDVAKEAMVTRYSSISTRKLSKALVILTQDKCMVATSDYDEFHKMVK 146

Query: 940  RQLLTHLLGPIAQKRNRIDRDIVVENTLNKVQASVKKSPLEPINFRKIFELELFSLGMKH 761
            R LLT++LG  AQ+R+R  RD +++N  ++  A VK +P + +NFR IFE ELF L +K 
Sbjct: 147  RYLLTNVLGANAQRRHRCHRDTLIKNISSRFHAHVKTNPEQTVNFRDIFESELFGLSLKE 206

Query: 760  ALGLDVDSIYVDELGITMSKEDMFQCIVIDPMAGSIEVDWRDFFPYLKWIPNRSLEKKVE 581
            ALG DV SIYVDELG T+S++++F+ +V+DPM G+I+VDWRDFFPYL+W+PN+S E K++
Sbjct: 207  ALGKDVQSIYVDELGTTLSRKEIFEVLVLDPMEGAIDVDWRDFFPYLRWVPNKSWEMKIQ 266

Query: 580  QMYIRKTAVIKALIKKTKTQSASKEGVPCXXXXXXXXXXXXXXXKQIHMLMWEVLIATAD 401
            QM+ R+ AV+ ALIK+ K + A  E V C               +QI ML+WE ++ T+D
Sbjct: 267  QMHFRRKAVMGALIKEQKKRIALGEEVNC--YLDFLSSESTLTDEQIRMLIWETIVETSD 324

Query: 400  TTLVATEWAMYEIARDSKRQQRLYEEISRVCGSNRITEEHLHQLPYLYAIFQETIRVYSP 221
            TT+VATEWA+YE+A++   Q  LY +I  VCGS +ITEEHL +LPYL A+F ETIR YSP
Sbjct: 325  TTMVATEWAIYELAKNPNCQDLLYRQIQNVCGSEKITEEHLSKLPYLNAVFHETIRKYSP 384

Query: 220  FPVTPLRYVHEDIELGGYFVPEGTEIAINIYGCNMDKSIWKNPEEWKPERFMEEESDTME 41
             PV PLRYVHED ELGGY++P G+EIAINIYGCNMDK+ W NPEEW PERF+    D+ +
Sbjct: 385  APVIPLRYVHEDTELGGYYIPAGSEIAINIYGCNMDKNRWVNPEEWNPERFLNGNYDSGD 444

Query: 40   LHRSLAFGGGKR 5
            LH+++AFG GKR
Sbjct: 445  LHKTMAFGAGKR 456


>ref|XP_002282197.1| PREDICTED: ent-kaurene oxidase, chloroplastic [Vitis vinifera]
            gi|302142385|emb|CBI19588.3| unnamed protein product
            [Vitis vinifera]
          Length = 508

 Score =  523 bits (1347), Expect = e-146
 Identities = 253/450 (56%), Positives = 338/450 (75%), Gaps = 4/450 (0%)
 Frame = -1

Query: 1339 LASTPIITLVVCG----FSLWFLKRFVKHNKKSPPNLRPLPVVAGGLPIVGNLLQLKEKK 1172
            + + P  T VV G      L F++RFV + K+S   L P+P + G LP++GNLLQLKEKK
Sbjct: 6    IEAVPFATAVVLGGISLVVLIFIRRFVSNRKRSVEGLPPVPDIPG-LPLIGNLLQLKEKK 64

Query: 1171 PHKIFLKWVETYGPVYSIRTGYNTIVVINSIDVAKEAMVTKFDSISTKKLSTAVKILADD 992
            PHK F +W ETYGP++SIRTG +T++V+NS +VAKEAMVT+F SIST+KLS A+KIL  D
Sbjct: 65   PHKTFARWAETYGPIFSIRTGASTMIVLNSSEVAKEAMVTRFSSISTRKLSNALKILTFD 124

Query: 991  KSMVATSDYDEFHKIAKRQLLTHLLGPIAQKRNRIDRDIVVENTLNKVQASVKKSPLEPI 812
            K MVATSDY++FHK+ K  +L ++LG  AQKR+R  RD ++EN    + A VK SPLEP+
Sbjct: 125  KCMVATSDYNDFHKMVKGFILRNVLGAPAQKRHRCHRDTLIENISKYLHAHVKTSPLEPV 184

Query: 811  NFRKIFELELFSLGMKHALGLDVDSIYVDELGITMSKEDMFQCIVIDPMAGSIEVDWRDF 632
              +KIFE E+F L +K ALG D++SIYV+ELG T+S+E++F  +V+DPMAG+IEVDWRDF
Sbjct: 185  VLKKIFESEIFGLALKQALGKDIESIYVEELGTTLSREEIFAVLVVDPMAGAIEVDWRDF 244

Query: 631  FPYLKWIPNRSLEKKVEQMYIRKTAVIKALIKKTKTQSASKEGVPCXXXXXXXXXXXXXX 452
            FPYL WIPN+S+E K+++M  R+ A++KALI + K +  S E   C              
Sbjct: 245  FPYLSWIPNKSMEMKIQRMDFRRGALMKALIGEQKKRIGSGEEKNC-YIDFLLSEATTLT 303

Query: 451  XKQIHMLMWEVLIATADTTLVATEWAMYEIARDSKRQQRLYEEISRVCGSNRITEEHLHQ 272
             KQI ML+WE +I  +DTTLV +EWAMYE+A+D  RQ+ LY EI + CGSN++TEE+L +
Sbjct: 304  EKQIAMLIWETIIEISDTTLVTSEWAMYELAKDPNRQEILYREIRKFCGSNKVTEENLSK 363

Query: 271  LPYLYAIFQETIRVYSPFPVTPLRYVHEDIELGGYFVPEGTEIAINIYGCNMDKSIWKNP 92
            LPYL ++F ET+R YSP P+ P+RY HED +LGGY +P G++IAINIYGCNM+K  W+NP
Sbjct: 364  LPYLNSVFHETLRKYSPAPIVPVRYAHEDTQLGGYHIPAGSQIAINIYGCNMNKKQWENP 423

Query: 91   EEWKPERFMEEESDTMELHRSLAFGGGKRV 2
            EEWKPERF++E+ D M+LH+++AFGGGKRV
Sbjct: 424  EEWKPERFLDEKYDLMDLHKTMAFGGGKRV 453


>gb|AFD54196.1| ent-kaurene oxidase [Vitis vinifera]
          Length = 508

 Score =  520 bits (1339), Expect = e-145
 Identities = 253/450 (56%), Positives = 338/450 (75%), Gaps = 4/450 (0%)
 Frame = -1

Query: 1339 LASTPIITLVVCG----FSLWFLKRFVKHNKKSPPNLRPLPVVAGGLPIVGNLLQLKEKK 1172
            + + P  T VV G      L F++RFV + K+S   L P+P + G LP++GNLLQLKEKK
Sbjct: 6    IEAVPFATAVVLGGISLVVLIFIRRFVSNRKRSVEGLPPVPDIPG-LPLIGNLLQLKEKK 64

Query: 1171 PHKIFLKWVETYGPVYSIRTGYNTIVVINSIDVAKEAMVTKFDSISTKKLSTAVKILADD 992
            PHK F +W ETYGP++SIRTG +T++V+NS +VAKEAMVT+F SIST+KLS A+KIL  D
Sbjct: 65   PHKTFARWAETYGPIFSIRTGASTMIVLNSSEVAKEAMVTRFSSISTRKLSNALKILTFD 124

Query: 991  KSMVATSDYDEFHKIAKRQLLTHLLGPIAQKRNRIDRDIVVENTLNKVQASVKKSPLEPI 812
            K MVATSDY++FHK+ K  +L ++LG  AQKR+R  RD ++EN    + A VK SPLEP+
Sbjct: 125  KCMVATSDYNDFHKMVKGFILRNVLGAPAQKRHRCHRDTLIENISKYLHAHVKTSPLEPV 184

Query: 811  NFRKIFELELFSLGMKHALGLDVDSIYVDELGITMSKEDMFQCIVIDPMAGSIEVDWRDF 632
              +KIFE E+F L +K ALG D++SIYV+ELG T+S+E++F  +V+DPMAG+IEVDWRDF
Sbjct: 185  VLKKIFESEIFGLALKQALGKDIESIYVEELGTTLSREEIFAVLVVDPMAGAIEVDWRDF 244

Query: 631  FPYLKWIPNRSLEKKVEQMYIRKTAVIKALIKKTKTQSASKEGVPCXXXXXXXXXXXXXX 452
            FPYL WIPN+S+E K+++M  R+ A++KALI + K +  S E                  
Sbjct: 245  FPYLSWIPNKSMEMKIQRMDFRRGALMKALIGEQKKRIGSGE-EKNSYIDFLLSEATTLT 303

Query: 451  XKQIHMLMWEVLIATADTTLVATEWAMYEIARDSKRQQRLYEEISRVCGSNRITEEHLHQ 272
             KQI ML+WE +I  +DTTLV +EWAMYE+A+D  RQ+ LY EI +VCGSN++TEE+L +
Sbjct: 304  EKQIAMLIWETIIEISDTTLVTSEWAMYELAKDPNRQEILYREIHKVCGSNKLTEENLSK 363

Query: 271  LPYLYAIFQETIRVYSPFPVTPLRYVHEDIELGGYFVPEGTEIAINIYGCNMDKSIWKNP 92
            LPYL ++F ET+R YSP P+ P+RY HED +LGGY +P G++IAINIYGCNM+K  W+NP
Sbjct: 364  LPYLNSVFHETLRKYSPAPMVPVRYAHEDTQLGGYHIPAGSQIAINIYGCNMNKKQWENP 423

Query: 91   EEWKPERFMEEESDTMELHRSLAFGGGKRV 2
            EEWKPERF++E+ D M+LH+++AFGGGKRV
Sbjct: 424  EEWKPERFLDEKYDLMDLHKTMAFGGGKRV 453


>gb|ACQ99375.1| ent-kaurene oxidase 1 [Coffea arabica]
          Length = 514

 Score =  506 bits (1303), Expect = e-141
 Identities = 247/446 (55%), Positives = 325/446 (72%)
 Frame = -1

Query: 1339 LASTPIITLVVCGFSLWFLKRFVKHNKKSPPNLRPLPVVAGGLPIVGNLLQLKEKKPHKI 1160
            L +  ++ +V+ G SL  LK FV   ++   +  PL     GLP++GNLLQLKEKKPH+ 
Sbjct: 15   LGTAVVVAVVLGGLSLLVLKAFVNAQRRKSSSNLPLVPEVPGLPLLGNLLQLKEKKPHRT 74

Query: 1159 FLKWVETYGPVYSIRTGYNTIVVINSIDVAKEAMVTKFDSISTKKLSTAVKILADDKSMV 980
            F ++ ETYGP+YSI+TG N IVV+NS DVAKEAMVT++ SIS++KLS A+ IL   KS++
Sbjct: 75   FARFAETYGPIYSIKTGANKIVVLNSNDVAKEAMVTRYSSISSRKLSKALTILTAGKSII 134

Query: 979  ATSDYDEFHKIAKRQLLTHLLGPIAQKRNRIDRDIVVENTLNKVQASVKKSPLEPINFRK 800
            A SDYDE++K+AK+ +L   LG  AQKR+R  RD + EN  NK+  S+ ++PLE +NFR 
Sbjct: 135  AMSDYDEYYKMAKKHILNSTLGTNAQKRHRAHRDALTENICNKLXTSLNENPLEAVNFRN 194

Query: 799  IFELELFSLGMKHALGLDVDSIYVDELGITMSKEDMFQCIVIDPMAGSIEVDWRDFFPYL 620
            I+  ELFSL +K  LG DV+S +V+E G T+SKE++ + +V DPM G++EVDWRDFFP+L
Sbjct: 195  IYLFELFSLALKEVLGEDVESTFVEEFGTTLSKEELLKVLVHDPMEGALEVDWRDFFPFL 254

Query: 619  KWIPNRSLEKKVEQMYIRKTAVIKALIKKTKTQSASKEGVPCXXXXXXXXXXXXXXXKQI 440
            +WIPN+S E K+ QM +R+ AV KALIKK K +  S + + C               KQI
Sbjct: 255  RWIPNKSFEDKIHQMDLRRGAVTKALIKKQKKRFESGQEIYC-YLDSILSDEKSFTEKQI 313

Query: 439  HMLMWEVLIATADTTLVATEWAMYEIARDSKRQQRLYEEISRVCGSNRITEEHLHQLPYL 260
             ML+WE +I T+DTTLV  EWAMYE+A+D  +Q RL+ +I  VCG N++TEE+L QLPYL
Sbjct: 314  MMLIWEAIIETSDTTLVTAEWAMYELAKDPVKQDRLFRDIKNVCGPNKVTEENLGQLPYL 373

Query: 259  YAIFQETIRVYSPFPVTPLRYVHEDIELGGYFVPEGTEIAINIYGCNMDKSIWKNPEEWK 80
             AIF ET+R +SP PV PLRYVHED ELGGY +P GTEIAIN+YGCNMDK  W+NPE+W 
Sbjct: 374  SAIFHETLRRHSPVPVVPLRYVHEDTELGGYHIPAGTEIAINLYGCNMDKKTWENPEQWI 433

Query: 79   PERFMEEESDTMELHRSLAFGGGKRV 2
            PERF++   D MELH+++AFGGGKRV
Sbjct: 434  PERFVDGLHDYMELHKTIAFGGGKRV 459