BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00006462
         (1152 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABB52074.1| putative beta-ring carotene hydroxylase [Daucus c...   464   e-128
ref|XP_002273581.1| PREDICTED: beta-carotene hydroxylase 2, chlo...   418   e-114
gb|ACN86365.1| beta-carotene hydroxylase [Diospyros kaki] gi|253...   415   e-113
dbj|BAI47580.1| beta-carotene hydroxylase [Ipomoea nil]               409   e-112
dbj|BAI47579.1| beta-carotene hydroxylase [Ipomoea obscura]           406   e-111

>gb|ABB52074.1| putative beta-ring carotene hydroxylase [Daucus carota subsp.
            sativus]
          Length = 309

 Score =  464 bits (1195), Expect = e-128
 Identities = 235/308 (76%), Positives = 249/308 (80%)
 Frame = -2

Query: 1058 MAAGIAVAYGSGSFLVTRNLLLGPKPISSATPTWLFSPSIHKLKTSLRLPKKGSTTLCFV 879
            MAAGI+ A  S SF + RN  LGP PI      WLF+PS+ KL  SLR  +K  TT+CFV
Sbjct: 1    MAAGISAASSSTSFSLGRNPFLGPNPI------WLFAPSVRKLNPSLRFKQKSLTTVCFV 54

Query: 878  VVGTKSELRNDDPEKPEERSYRDDPQNTVMXXXXXXXXXXXXXXKMARKKSERFTYLVAA 699
            V     E RND   KPE  + RD+     +                ARKKSERFTYLVAA
Sbjct: 55   V-----EPRNDSSGKPENNADRDEVSREEIEAGSCSVRVEERR---ARKKSERFTYLVAA 106

Query: 698  VMSSLGITSMAVLAVYYRFSWQLEGGQIPYSEMFGTFALSVGAAVGMEFWARWAHEALWH 519
            VMSSLGITSMAVLAVYYRFSWQ+EGG+IPYSEM GTFALSVGAAVGMEFWARWAHEALWH
Sbjct: 107  VMSSLGITSMAVLAVYYRFSWQMEGGEIPYSEMIGTFALSVGAAVGMEFWARWAHEALWH 166

Query: 518  ASLWHMHESHHKPREGPFELNDVFAIMNAAPAIALLAYGFFHKGFFPGLCFGAGLGITVF 339
            ASLWHMHESHHKPREG FELNDVFAIMNA PAIALLAYGFFHKG+FPGLCFGAGLGITVF
Sbjct: 167  ASLWHMHESHHKPREGAFELNDVFAIMNAVPAIALLAYGFFHKGYFPGLCFGAGLGITVF 226

Query: 338  GMAYMFVHDGLVHKRFPVGPIADVPYFRKVAAAHQLHHMEKFKGVPYGLFLGPKEVEDVG 159
            G+AYMFVHDGLVHKRF VGPIADVPYFRKVAAAHQLHHMEKFKGVPYGLFLGPKEVEDVG
Sbjct: 227  GIAYMFVHDGLVHKRFAVGPIADVPYFRKVAAAHQLHHMEKFKGVPYGLFLGPKEVEDVG 286

Query: 158  GHEALEME 135
            GHEALE+E
Sbjct: 287  GHEALELE 294


>ref|XP_002273581.1| PREDICTED: beta-carotene hydroxylase 2, chloroplastic isoform 1
            [Vitis vinifera]
          Length = 306

 Score =  418 bits (1074), Expect = e-114
 Identities = 212/308 (68%), Positives = 234/308 (75%)
 Frame = -2

Query: 1058 MAAGIAVAYGSGSFLVTRNLLLGPKPISSATPTWLFSPSIHKLKTSLRLPKKGSTTLCFV 879
            MA  I+VA  S S  + RN  LGPKP S  TPT LF PSI + +   R  KK   T+CFV
Sbjct: 1    MAVEISVATSSRSPRLGRNPFLGPKPTSPFTPTSLFIPSIRRHENIFRCRKKTRLTVCFV 60

Query: 878  VVGTKSELRNDDPEKPEERSYRDDPQNTVMXXXXXXXXXXXXXXKMARKKSERFTYLVAA 699
            V               EE+   +  +N                 K+ARK+SER TYLVAA
Sbjct: 61   V--------------EEEKLSTEVVENRSEETLASQISAARVAEKLARKRSERLTYLVAA 106

Query: 698  VMSSLGITSMAVLAVYYRFSWQLEGGQIPYSEMFGTFALSVGAAVGMEFWARWAHEALWH 519
            VMSS GITSMAV+AVYYRFSWQ+EGG++P SEMFGTFALSVGAAVGMEFWARWAH+ALWH
Sbjct: 107  VMSSFGITSMAVMAVYYRFSWQMEGGEVPLSEMFGTFALSVGAAVGMEFWARWAHKALWH 166

Query: 518  ASLWHMHESHHKPREGPFELNDVFAIMNAAPAIALLAYGFFHKGFFPGLCFGAGLGITVF 339
            ASLWHMHESHHKPREGPFELNDVFAI NA PAIALL+YGFFHKG  PGLCFGAGLGITVF
Sbjct: 167  ASLWHMHESHHKPREGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVF 226

Query: 338  GMAYMFVHDGLVHKRFPVGPIADVPYFRKVAAAHQLHHMEKFKGVPYGLFLGPKEVEDVG 159
            GMAYMFVHDGLVH+RFPVGPIA+VPYFR+VAAAHQLHH +KF GVPYGLFLGPKEVE+VG
Sbjct: 227  GMAYMFVHDGLVHRRFPVGPIANVPYFRRVAAAHQLHHSDKFNGVPYGLFLGPKEVEEVG 286

Query: 158  GHEALEME 135
            G + LE E
Sbjct: 287  GKDELEKE 294


>gb|ACN86365.1| beta-carotene hydroxylase [Diospyros kaki]
            gi|253987308|gb|ACT52160.1| beta-carotene hydroxylase
            [Diospyros kaki]
          Length = 312

 Score =  415 bits (1066), Expect = e-113
 Identities = 214/311 (68%), Positives = 239/311 (76%), Gaps = 3/311 (0%)
 Frame = -2

Query: 1058 MAAGIAVAY-GSGSFLVTRNL-LLGPKPISS-ATPTWLFSPSIHKLKTSLRLPKKGSTTL 888
            M AGI++A  G  SF++TR   LLGP P S+ +TPT LFS         LR   + + T+
Sbjct: 1    MPAGISIAASGPRSFILTRKYPLLGPGPESTISTPTCLFSLPARPFGAVLRSGSRTAKTV 60

Query: 887  CFVVVGTKSELRNDDPEKPEERSYRDDPQNTVMXXXXXXXXXXXXXXKMARKKSERFTYL 708
            CFVV   +   + +  ++ E      D + +                 +ARK+SERFTYL
Sbjct: 61   CFVVKEEELAAQLEAGDQDETSGVETDKRISAARVEEK----------LARKRSERFTYL 110

Query: 707  VAAVMSSLGITSMAVLAVYYRFSWQLEGGQIPYSEMFGTFALSVGAAVGMEFWARWAHEA 528
            VAAVMSS GITSMAVLAVYYRFSWQ+EGG+IPYSEMFGTFALSVGAAVGMEFWARWAH A
Sbjct: 111  VAAVMSSFGITSMAVLAVYYRFSWQMEGGEIPYSEMFGTFALSVGAAVGMEFWARWAHRA 170

Query: 527  LWHASLWHMHESHHKPREGPFELNDVFAIMNAAPAIALLAYGFFHKGFFPGLCFGAGLGI 348
            LWHASLWHMHESHHKP EGPFELNDVFAI+NA PAIALL+YGFFHKG  PGLCFGAGLGI
Sbjct: 171  LWHASLWHMHESHHKPIEGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGI 230

Query: 347  TVFGMAYMFVHDGLVHKRFPVGPIADVPYFRKVAAAHQLHHMEKFKGVPYGLFLGPKEVE 168
            TVFGMAYMFVHDGLVHKRFPVGPIADVPYFR+VAAAHQLHH +KF GVPYGLFLGPKEVE
Sbjct: 231  TVFGMAYMFVHDGLVHKRFPVGPIADVPYFRRVAAAHQLHHSDKFNGVPYGLFLGPKEVE 290

Query: 167  DVGGHEALEME 135
            +VGG E LE E
Sbjct: 291  EVGGDEELEKE 301


>dbj|BAI47580.1| beta-carotene hydroxylase [Ipomoea nil]
          Length = 310

 Score =  409 bits (1050), Expect = e-112
 Identities = 208/314 (66%), Positives = 241/314 (76%), Gaps = 6/314 (1%)
 Frame = -2

Query: 1058 MAAGIAVAYGSGSFLVTRNLLLGPKPISSATPTWLFSPSIHKLKTS-LRLPKKGSTTLCF 882
            MA GI++A  S +    +  L+ P   S++ P+ LFSP   + ++S LR  +K S T+CF
Sbjct: 1    MAVGISIAASSRTVYSCQFSLVRPATHSASPPSLLFSPLSRRFRSSVLRSRRKPSLTVCF 60

Query: 881  VVVGTKSE-----LRNDDPEKPEERSYRDDPQNTVMXXXXXXXXXXXXXXKMARKKSERF 717
            V+   K E     +R ++ E+  E+                         K+ARK+SERF
Sbjct: 61   VLEDEKLESGGVEIRAEEIERAIEKQISAS----------------RLAEKLARKRSERF 104

Query: 716  TYLVAAVMSSLGITSMAVLAVYYRFSWQLEGGQIPYSEMFGTFALSVGAAVGMEFWARWA 537
            TYLVAAVMSSLGITSMAVLAVYYRF+WQ+EGG++PY+EMFGTFALSVGAAVGMEFWARWA
Sbjct: 105  TYLVAAVMSSLGITSMAVLAVYYRFAWQMEGGEVPYTEMFGTFALSVGAAVGMEFWARWA 164

Query: 536  HEALWHASLWHMHESHHKPREGPFELNDVFAIMNAAPAIALLAYGFFHKGFFPGLCFGAG 357
            H ALWHASLWHMHESHH+PREGPFELNDVFAI NA PAIALL+YGFFHKG  PGLCFGAG
Sbjct: 165  HRALWHASLWHMHESHHRPREGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAG 224

Query: 356  LGITVFGMAYMFVHDGLVHKRFPVGPIADVPYFRKVAAAHQLHHMEKFKGVPYGLFLGPK 177
            LGITVFGMAYMFVHDGLVHKRFPVGPIADVPYFR+VAAAHQLHH +KF GVPYGLFLGPK
Sbjct: 225  LGITVFGMAYMFVHDGLVHKRFPVGPIADVPYFRRVAAAHQLHHTDKFNGVPYGLFLGPK 284

Query: 176  EVEDVGGHEALEME 135
            E+E+VGG   LE+E
Sbjct: 285  ELEEVGGLNDLEVE 298


>dbj|BAI47579.1| beta-carotene hydroxylase [Ipomoea obscura]
          Length = 309

 Score =  406 bits (1044), Expect = e-111
 Identities = 208/313 (66%), Positives = 239/313 (76%), Gaps = 5/313 (1%)
 Frame = -2

Query: 1058 MAAGIAVAYGSGSFLVTRNLLLGPKPISSATPTWLFSPSIHKLKTS-LRLPKKGSTTLCF 882
            MA GI++A  SG+    +  L+ P   S++ P+ LFSP   + ++S L   +K   T+CF
Sbjct: 1    MAVGISIAASSGNVYNCQFSLVRPATHSASPPSLLFSPLSRRFRSSVLSSRRKPRLTVCF 60

Query: 881  VVVGTKSE----LRNDDPEKPEERSYRDDPQNTVMXXXXXXXXXXXXXXKMARKKSERFT 714
            V+   K E    +R ++ EK  E+                         K+ARK+SER T
Sbjct: 61   VLEDEKLESGVQIRAEEIEKAIEKQISAS----------------RLAEKLARKRSERST 104

Query: 713  YLVAAVMSSLGITSMAVLAVYYRFSWQLEGGQIPYSEMFGTFALSVGAAVGMEFWARWAH 534
            YLVAAVMSSLGITSMAVLAVYYRF+WQ+EGG +PY+EMFGTFALSVGAAVGMEFWARWAH
Sbjct: 105  YLVAAVMSSLGITSMAVLAVYYRFAWQMEGGAVPYTEMFGTFALSVGAAVGMEFWARWAH 164

Query: 533  EALWHASLWHMHESHHKPREGPFELNDVFAIMNAAPAIALLAYGFFHKGFFPGLCFGAGL 354
             ALWHASLWHMHESHHKPREGPFELNDVFAI+NA PAIALL+YGFFHKG  PGLCFGAGL
Sbjct: 165  RALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGL 224

Query: 353  GITVFGMAYMFVHDGLVHKRFPVGPIADVPYFRKVAAAHQLHHMEKFKGVPYGLFLGPKE 174
            GITVFGMAYMFVHDGLVHKRFPVGPIADVPYFR+VAAAHQLHH +KF GVPYGLFLGPKE
Sbjct: 225  GITVFGMAYMFVHDGLVHKRFPVGPIADVPYFRRVAAAHQLHHTDKFNGVPYGLFLGPKE 284

Query: 173  VEDVGGHEALEME 135
            +E+VGG   LE+E
Sbjct: 285  LEEVGGLNDLEVE 297