BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00006416
         (1592 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002298873.1| predicted protein [Populus trichocarpa] gi|2...   583   e-164
ref|XP_002522774.1| E3 ubiquitin protein ligase upl2, putative [...   581   e-163
ref|XP_002268896.2| PREDICTED: E3 ubiquitin-protein ligase UPL1-...   577   e-162
ref|XP_002316781.1| predicted protein [Populus trichocarpa] gi|2...   568   e-159
ref|XP_004166446.1| PREDICTED: LOW QUALITY PROTEIN: E3 ubiquitin...   565   e-159

>ref|XP_002298873.1| predicted protein [Populus trichocarpa] gi|222846131|gb|EEE83678.1|
            predicted protein [Populus trichocarpa]
          Length = 3728

 Score =  583 bits (1504), Expect = e-164
 Identities = 301/436 (69%), Positives = 343/436 (78%), Gaps = 2/436 (0%)
 Frame = +1

Query: 289  KLKRKRAVEVPPSIRSFINNVIATPLELIGEPLRSFVWDFNKGDFHQWVDLFNHFDSFFE 468
            KLKR+R+ EVPP I+SFINNV  TPLE I EPL+ FVW+F+KGDFH WVDLFNHFDS+FE
Sbjct: 3    KLKRRRSSEVPPKIKSFINNVTTTPLENIEEPLKGFVWEFDKGDFHHWVDLFNHFDSYFE 62

Query: 469  KYIKSRNDLQVEDNFVALDAPFPKDAVLQILRVTRIILENCTNKHFYSSYEQHLSSLLSS 648
            K+IK R DLQVEDNF+  D PFP++AVLQIL V RIILENCTNKHFYSSYEQHLS+LLSS
Sbjct: 63   KHIKPRRDLQVEDNFLESDPPFPREAVLQILCVIRIILENCTNKHFYSSYEQHLSNLLSS 122

Query: 649  TDADVVEASLQTLAAFLKKAVGKYVIRDSGLHSKLFAFAQGWGGKEEGLGLVACSVQNAL 828
            TDADV+EA LQTLAAFLKK +G+Y IRD+ L++KLF+ AQGWGGK+EGLGL+A + QN  
Sbjct: 123  TDADVLEACLQTLAAFLKKTLGRYSIRDTSLNTKLFSLAQGWGGKDEGLGLIASTAQNGC 182

Query: 829  DQVAYDVGSTLHFEFYA-DDTSNAFSASEQPAQGLQIIHLPNVNSHQVSDSELFQKLVME 1005
            D VAY++G TLHFEFYA D+ S+  SA+E+  QGLQ IHLPNVN+   +D EL  KLV+E
Sbjct: 183  DPVAYELGCTLHFEFYALDELSSQVSATERSTQGLQTIHLPNVNACPETDLELLNKLVVE 242

Query: 1006 YKVPPXXXXXXXXXXXXXXXXSSLASRQQYTCIRLYALMVLVQSCSATEDLVSFFNTEPE 1185
            YKVPP                 SLASRQQYTCIRLYA +VLVQ+ S  +DLVSFFN+EPE
Sbjct: 243  YKVPPSLRFSLLTRLRFARAFGSLASRQQYTCIRLYAFIVLVQASSDADDLVSFFNSEPE 302

Query: 1186 FINELVTLLSYEDAVPEKIRILSLLCLVALCQDRSRQQTVLIAVTSGGHRGILSSLMQKV 1365
            FINELV+LLSYED VPEKIRIL LL LVAL QDRSRQ TVL AVTSGGHRGILSSLMQK 
Sbjct: 303  FINELVSLLSYEDEVPEKIRILCLLSLVALSQDRSRQSTVLAAVTSGGHRGILSSLMQKT 362

Query: 1366 IDAV-SNSSKWSVIFAEAXXXXXXXXXXXXXGCSAMREAGFIXXXXXXXXXXXXQHLHLV 1542
            ID+V S++SKWSV+F+EA             GCSAMREAGFI            QHLHLV
Sbjct: 363  IDSVISDTSKWSVVFSEALLSLVTVLVSSSSGCSAMREAGFIPTLLPLLKDTDPQHLHLV 422

Query: 1543 STAVHILEAFMDYSNP 1590
            +TAVHILEAFMDYSNP
Sbjct: 423  ATAVHILEAFMDYSNP 438


>ref|XP_002522774.1| E3 ubiquitin protein ligase upl2, putative [Ricinus communis]
            gi|223538012|gb|EEF39625.1| E3 ubiquitin protein ligase
            upl2, putative [Ricinus communis]
          Length = 3691

 Score =  581 bits (1497), Expect = e-163
 Identities = 299/437 (68%), Positives = 342/437 (78%), Gaps = 2/437 (0%)
 Frame = +1

Query: 286  MKLKRKRAVEVPPSIRSFINNVIATPLELIGEPLRSFVWDFNKGDFHQWVDLFNHFDSFF 465
            MKLKR+R++EVPP I+SFIN+V +TPLE I EPL+ FVW+F+KGDFH WVDLFNHFDSFF
Sbjct: 1    MKLKRRRSLEVPPKIKSFINSVTSTPLENIEEPLKGFVWEFDKGDFHHWVDLFNHFDSFF 60

Query: 466  EKYIKSRNDLQVEDNFVALDAPFPKDAVLQILRVTRIILENCTNKHFYSSYEQHLSSLLS 645
            EK+IK R DLQVEDNF+  D PFP++AVLQILRV RIILENCTNKHFYSSYEQHLS LL+
Sbjct: 61   EKHIKPRKDLQVEDNFLESDPPFPREAVLQILRVIRIILENCTNKHFYSSYEQHLSFLLA 120

Query: 646  STDADVVEASLQTLAAFLKKAVGKYVIRDSGLHSKLFAFAQGWGGKEEGLGLVACSVQNA 825
            STDADV+EA LQTLAAFLKK +GKY IRD+ L++KLF+ AQGWGGKEEGLGL+AC+V+N 
Sbjct: 121  STDADVIEACLQTLAAFLKKTIGKYSIRDTLLNAKLFSLAQGWGGKEEGLGLIACTVENG 180

Query: 826  LDQVAYDVGSTLHFEFY-ADDTSNAFSASEQPAQGLQIIHLPNVNSHQVSDSELFQKLVM 1002
             D VAY++G TLHFEFY  D++SN   A+ Q  QGL+ IHLPNVN+ Q +D +L  KLV 
Sbjct: 181  CDPVAYELGCTLHFEFYQLDESSNESRANGQSNQGLKTIHLPNVNTCQETDLQLLNKLVA 240

Query: 1003 EYKVPPXXXXXXXXXXXXXXXXSSLASRQQYTCIRLYALMVLVQSCSATEDLVSFFNTEP 1182
            EYKVP                  SLAS+QQYTCIRLYA +VLVQ+ S  +DLVSFFN+EP
Sbjct: 241  EYKVPSSLRFSLLTRLRFARAFGSLASQQQYTCIRLYAFIVLVQASSDADDLVSFFNSEP 300

Query: 1183 EFINELVTLLSYEDAVPEKIRILSLLCLVALCQDRSRQQTVLIAVTSGGHRGILSSLMQK 1362
            EF+NELV+LLSYEDAVPEKIRIL LL LVAL QDRSRQ  VL AVTSGGHRGILSSLMQK
Sbjct: 301  EFVNELVSLLSYEDAVPEKIRILCLLSLVALSQDRSRQPAVLAAVTSGGHRGILSSLMQK 360

Query: 1363 VIDAV-SNSSKWSVIFAEAXXXXXXXXXXXXXGCSAMREAGFIXXXXXXXXXXXXQHLHL 1539
             ID+V S +SKWSV+FAEA             GCSAMREAGFI            QHLHL
Sbjct: 361  AIDSVISGTSKWSVVFAEALLSLVTVLVSSSSGCSAMREAGFIPTLLPLLKDTDPQHLHL 420

Query: 1540 VSTAVHILEAFMDYSNP 1590
            V TAVHILE FMD+SNP
Sbjct: 421  VGTAVHILETFMDFSNP 437


>ref|XP_002268896.2| PREDICTED: E3 ubiquitin-protein ligase UPL1-like [Vitis vinifera]
          Length = 4116

 Score =  577 bits (1486), Expect = e-162
 Identities = 298/426 (69%), Positives = 333/426 (78%), Gaps = 2/426 (0%)
 Frame = +1

Query: 319  PPSIRSFINNVIATPLELIGEPLRSFVWDFNKGDFHQWVDLFNHFDSFFEKYIKSRNDLQ 498
            PP IRSFIN V +TPLE I EPL+ F+W+F+KGDFH WVDLFNHFDSFFEK+IK R DLQ
Sbjct: 374  PPKIRSFINGVTSTPLENIEEPLKCFIWEFDKGDFHHWVDLFNHFDSFFEKHIKPRKDLQ 433

Query: 499  VEDNFVALDAPFPKDAVLQILRVTRIILENCTNKHFYSSYEQHLSSLLSSTDADVVEASL 678
            VEDNF+  D PFP++AVLQILRV RIILENCTNKHFYSSYEQHLS+LL+STDADVVEA L
Sbjct: 434  VEDNFLESDPPFPREAVLQILRVIRIILENCTNKHFYSSYEQHLSALLASTDADVVEACL 493

Query: 679  QTLAAFLKKAVGKYVIRDSGLHSKLFAFAQGWGGKEEGLGLVACSVQNALDQVAYDVGST 858
            QTLAAFLKK++GKY IRD+ L+SKLFAFAQGWGGKEEGLGL+ACSVQ+  DQ+AYD+G T
Sbjct: 494  QTLAAFLKKSIGKYPIRDASLNSKLFAFAQGWGGKEEGLGLIACSVQDGCDQIAYDLGCT 553

Query: 859  LHFEFYA-DDTSNAFSASEQPAQGLQIIHLPNVNSHQVSDSELFQKLVMEYKVPPXXXXX 1035
            LHFEFYA ++ SN    SE+ AQGLQIIHLPN+N+ Q +D EL  KLV+EY+VP      
Sbjct: 554  LHFEFYAVNEPSNGQPGSEKSAQGLQIIHLPNINTCQETDLELLNKLVIEYEVPTSLRFS 613

Query: 1036 XXXXXXXXXXXSSLASRQQYTCIRLYALMVLVQSCSATEDLVSFFNTEPEFINELVTLLS 1215
                        SLA+RQQYTCIRLYA MVLVQS S  +DL SFF   PE  NELV+LLS
Sbjct: 614  LLTRLRFARAFGSLAARQQYTCIRLYAFMVLVQSGSDADDLASFFTAVPEVTNELVSLLS 673

Query: 1216 YEDAVPEKIRILSLLCLVALCQDRSRQQTVLIAVTSGGHRGILSSLMQKVIDAV-SNSSK 1392
            YEDA+P KIRILSL  L ALCQDRSRQ +VL AVTSGGHRGIL SLMQK ID+V SN+SK
Sbjct: 674  YEDAIPIKIRILSLSSLAALCQDRSRQPSVLNAVTSGGHRGILPSLMQKAIDSVISNNSK 733

Query: 1393 WSVIFAEAXXXXXXXXXXXXXGCSAMREAGFIXXXXXXXXXXXXQHLHLVSTAVHILEAF 1572
            WSV+FAEA             GCSAMREAGFI            QHLHLVSTAVHILEAF
Sbjct: 734  WSVVFAEALLSVVTALVSSSSGCSAMREAGFIPTLLPLLKDTEPQHLHLVSTAVHILEAF 793

Query: 1573 MDYSNP 1590
            MDYSNP
Sbjct: 794  MDYSNP 799


>ref|XP_002316781.1| predicted protein [Populus trichocarpa] gi|222859846|gb|EEE97393.1|
            predicted protein [Populus trichocarpa]
          Length = 3663

 Score =  568 bits (1465), Expect = e-159
 Identities = 294/436 (67%), Positives = 335/436 (76%), Gaps = 2/436 (0%)
 Frame = +1

Query: 289  KLKRKRAVEVPPSIRSFINNVIATPLELIGEPLRSFVWDFNKGDFHQWVDLFNHFDSFFE 468
            KLK++R+ EVPP I+S IN VI +PLE I EPL+ FVW+F+KGDFH WVDLFNHFDS+FE
Sbjct: 3    KLKKRRSTEVPPKIKSIINGVITSPLENIEEPLKGFVWEFDKGDFHHWVDLFNHFDSYFE 62

Query: 469  KYIKSRNDLQVEDNFVALDAPFPKDAVLQILRVTRIILENCTNKHFYSSYEQHLSSLLSS 648
            K+IK R DLQVEDNF+  D PFP++AVLQIL V RIILENCTNKHFYSSYEQHLS+LL+S
Sbjct: 63   KHIKPRKDLQVEDNFLESDPPFPREAVLQILHVIRIILENCTNKHFYSSYEQHLSNLLAS 122

Query: 649  TDADVVEASLQTLAAFLKKAVGKYVIRDSGLHSKLFAFAQGWGGKEEGLGLVACSVQNAL 828
            TDAD+VEA LQTLAAFL K +G+Y IRD  L++KLF+ AQGWGGK+EGLGLVA + QN  
Sbjct: 123  TDADIVEACLQTLAAFLNKTLGRYSIRDLSLNTKLFSLAQGWGGKDEGLGLVASTTQNGC 182

Query: 829  DQVAYDVGSTLHFEFYA-DDTSNAFSASEQPAQGLQIIHLPNVNSHQVSDSELFQKLVME 1005
            D VAY++G TLHFEFYA ++ S+ FSA EQP QGLQIIHLPNV++   +D EL  KLV+E
Sbjct: 183  DPVAYELGCTLHFEFYALNELSSQFSAIEQPTQGLQIIHLPNVDTCPETDCELLNKLVVE 242

Query: 1006 YKVPPXXXXXXXXXXXXXXXXSSLASRQQYTCIRLYALMVLVQSCSATEDLVSFFNTEPE 1185
            YKVPP                  L SR  YTCIRLYA +VLVQ+ S  +DLVSFFN+EPE
Sbjct: 243  YKVPPSLRFSLLTRLRFARAFRPLVSRHLYTCIRLYAFIVLVQASSDADDLVSFFNSEPE 302

Query: 1186 FINELVTLLSYEDAVPEKIRILSLLCLVALCQDRSRQQTVLIAVTSGGHRGILSSLMQKV 1365
            F+NELV+LLSYED VPEKIRIL LL LVAL QDRSRQ TVL AVTS GHRGILSSLMQK 
Sbjct: 303  FVNELVSLLSYEDEVPEKIRILCLLSLVALSQDRSRQSTVLAAVTSSGHRGILSSLMQKA 362

Query: 1366 IDAV-SNSSKWSVIFAEAXXXXXXXXXXXXXGCSAMREAGFIXXXXXXXXXXXXQHLHLV 1542
            ID+V S+SSKWSV FAEA             GCSAMREAGFI            QHLHLV
Sbjct: 363  IDSVISDSSKWSVDFAEALLSLVTVLVSSSSGCSAMREAGFIPTLLPLLKDTDPQHLHLV 422

Query: 1543 STAVHILEAFMDYSNP 1590
            + AVHILEAFMDYSNP
Sbjct: 423  AAAVHILEAFMDYSNP 438


>ref|XP_004166446.1| PREDICTED: LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase UPL1-like
            [Cucumis sativus]
          Length = 3692

 Score =  565 bits (1457), Expect = e-159
 Identities = 289/436 (66%), Positives = 336/436 (77%), Gaps = 1/436 (0%)
 Frame = +1

Query: 286  MKLKRKRAVEVPPSIRSFINNVIATPLELIGEPLRSFVWDFNKGDFHQWVDLFNHFDSFF 465
            MK+KR+RA+EVPP IRSFINNV + PLE I EPL+ FVW+F+KGDFH WVDLFNHFDSFF
Sbjct: 1    MKMKRRRALEVPPKIRSFINNVTSVPLEDIEEPLKGFVWEFDKGDFHHWVDLFNHFDSFF 60

Query: 466  EKYIKSRNDLQVEDNFVALDAPFPKDAVLQILRVTRIILENCTNKHFYSSYEQHLSSLLS 645
            EK+IK+R DLQVEDNF+  D PFP++AVLQILRV RIIL NCTNKHFYSSYEQHLS LL+
Sbjct: 61   EKHIKTRKDLQVEDNFLGSDPPFPREAVLQILRVIRIILGNCTNKHFYSSYEQHLSCLLA 120

Query: 646  STDADVVEASLQTLAAFLKKAVGKYVIRDSGLHSKLFAFAQGWGGKEEGLGLVACSVQNA 825
            STDAD+VEA LQTL+AFLKK++GKY IRD+ L+SKLFA AQGWGGKEEGLGL+AC++QN 
Sbjct: 121  STDADIVEACLQTLSAFLKKSIGKYSIRDASLNSKLFALAQGWGGKEEGLGLIACALQNG 180

Query: 826  LDQVAYDVGSTLHFEFYADDTSNAFSASEQPAQGLQIIHLPNVNSHQVSDSELFQKLVME 1005
              QV +++G TLHFEFYA + S +   +EQ  +GLQIIHLPNV+S   +D EL  KL+ E
Sbjct: 181  CSQVTHELGCTLHFEFYALNESGS-DITEQSTKGLQIIHLPNVSSCLETDLELLSKLIAE 239

Query: 1006 YKVPPXXXXXXXXXXXXXXXXSSLASRQQYTCIRLYALMVLVQSCSATEDLVSFFNTEPE 1185
            + VP                  SL++RQQYTCIRLYA +VLVQ+    EDLVSFFN+EPE
Sbjct: 240  FNVPTSLRFSLLTRMRFARAFHSLSARQQYTCIRLYAFIVLVQASGDAEDLVSFFNSEPE 299

Query: 1186 FINELVTLLSYEDAVPEKIRILSLLCLVALCQDRSRQQTVLIAVTSGGHRGILSSLMQKV 1365
            F+NELV LLSYED VP KIRI  LL LVALCQDRSRQ TVL AVTSGGHRGILSSLMQK 
Sbjct: 300  FVNELVGLLSYEDEVPVKIRIHCLLSLVALCQDRSRQPTVLSAVTSGGHRGILSSLMQKA 359

Query: 1366 IDAV-SNSSKWSVIFAEAXXXXXXXXXXXXXGCSAMREAGFIXXXXXXXXXXXXQHLHLV 1542
            +D+V ++++ WS++FAE              GCSAMREAGFI            QHLHLV
Sbjct: 360  VDSVITDTTNWSLLFAETLLSLVTVLVSSSSGCSAMREAGFIPTLLPLLKDTNPQHLHLV 419

Query: 1543 STAVHILEAFMDYSNP 1590
            STAVHILE FMDYSNP
Sbjct: 420  STAVHILEVFMDYSNP 435