BLASTX nr result
ID: Bupleurum21_contig00006363
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00006363
(1862 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_001234282.1| SBT1 protein precursor [Solanum lycopersicum... 607 0.0
ref|XP_002283279.2| PREDICTED: subtilisin-like protease-like [Vi... 607 0.0
ref|XP_002301156.1| predicted protein [Populus trichocarpa] gi|2... 605 0.0
ref|XP_002510119.1| Xylem serine proteinase 1 precursor, putativ... 598 0.0
ref|XP_004142884.1| PREDICTED: subtilisin-like protease-like iso... 578 e-178
>ref|NP_001234282.1| SBT1 protein precursor [Solanum lycopersicum]
gi|1771160|emb|CAA67429.1| SBT1 [Solanum lycopersicum]
gi|3687305|emb|CAA06999.1| subtilisin-like protease
[Solanum lycopersicum]
Length = 766
Score = 607 bits (1566), Expect(2) = 0.0
Identities = 310/482 (64%), Positives = 367/482 (76%), Gaps = 6/482 (1%)
Frame = -2
Query: 1636 LVICCYGYAEERTMPKKTYIVHMDKSSMPLDYDDHLQWYDSSIKSVSNSANMLYTYNNVI 1457
+V+ + + + R KKTYI+HMDK +MP D+DDH QWYDSS+KSVS SANMLYTYN+VI
Sbjct: 13 MVVLFHVFVDARQNQKKTYIIHMDKFNMPADFDDHTQWYDSSLKSVSKSANMLYTYNSVI 72
Query: 1456 HGFSTRLTAAEAESLKGQPGILLVQEEMIYQLHTTRTPEFLGLD--MSTAVYLEAGAVSE 1283
HG+ST+LTA EA++L QPGILLV EE+IY+LHTTR+P FLGL+ S + + + A SE
Sbjct: 73 HGYSTQLTADEAKALAQQPGILLVHEEVIYELHTTRSPTFLGLEGRESRSFFPQTEARSE 132
Query: 1282 VIVGVLDTGVWPESRSFDDTGLGPIPSRWKGVCEVSKSFSISSCNKKLIGARSFSEGYEA 1103
VI+GVLDTGVWPES+SFDDTGLG +P+ WKG C+ K+F SSCN+KLIGAR FS+GYEA
Sbjct: 133 VIIGVLDTGVWPESKSFDDTGLGQVPASWKGKCQTGKNFDASSCNRKLIGARFFSQGYEA 192
Query: 1102 AFGPVDETMESKSPRXXXXXXXXXXXXXXXXAVTRASLFNYAMGTARGMATLARVAAYKV 923
AFG +DET+ESKSPR VT ASL YA GTARGMA+ ARVAAYKV
Sbjct: 193 AFGAIDETIESKSPRDDEGHGTHTATTAAGSVVTGASLLGYATGTARGMASHARVAAYKV 252
Query: 922 CWIGGCFSSDILAGLEMAXXXXXXXXXXXXXXXXXDYFQDTVAVGAFSAMSHGIFVSCXX 743
CW GGCFSSDILAG++ A DY +D VA+GAFSA S GIFVSC
Sbjct: 253 CWTGGCFSSDILAGMDQAVIDGVNVLSLSLGGTISDYHRDIVAIGAFSAASQGIFVSCSA 312
Query: 742 XXXXXXXXXXXNVAPWITTVGAGTLDRNFPANAILGNGKKFSGVSLYGGKPLSDSLVPLV 563
NVAPWITTVGAGT+DR FPA +GNGKK +GVSLY GK L S++PLV
Sbjct: 313 GNGGPSSGTLSNVAPWITTVGAGTMDREFPAYIGIGNGKKLNGVSLYSGKALPSSVMPLV 372
Query: 562 YATNTS----GSICTTGSLDHEKVAGKIVVCDRGVNSRAQKGVVVRDAGGVGMILANTDS 395
YA N S G++CT+GSL EKVAGKIVVCDRG+N+RAQKG+VV+DAGG+GMILANTD+
Sbjct: 373 YAGNVSQSSNGNLCTSGSLIPEKVAGKIVVCDRGMNARAQKGLVVKDAGGIGMILANTDT 432
Query: 394 YGEELVADAHLIPTAAVGQTAGDAIKKYISSDPKPMATILFGGTQLGIQPSPVVAAFSSR 215
YG+ELVADAHLIPTAAVGQTAG+ IK+YI+S+ P ATI FGGT+LG+QPSPVVAAFSSR
Sbjct: 433 YGDELVADAHLIPTAAVGQTAGNLIKQYIASNSNPTATIAFGGTKLGVQPSPVVAAFSSR 492
Query: 214 GP 209
GP
Sbjct: 493 GP 494
Score = 84.7 bits (208), Expect(2) = 0.0
Identities = 37/46 (80%), Positives = 43/46 (93%)
Frame = -1
Query: 140 PITPEILKPDFIAPGVNIIAGWTGKVGPTALKDDTRHVDFNIISGT 3
PITP++LKPD IAPGVNI+AGWTGKVGPT L++DTR+V FNIISGT
Sbjct: 496 PITPDVLKPDLIAPGVNILAGWTGKVGPTGLQEDTRNVGFNIISGT 541
>ref|XP_002283279.2| PREDICTED: subtilisin-like protease-like [Vitis vinifera]
Length = 765
Score = 607 bits (1565), Expect(2) = 0.0
Identities = 316/493 (64%), Positives = 364/493 (73%), Gaps = 7/493 (1%)
Frame = -2
Query: 1666 MKLWHISIATLVICCYGY--AEE-RTMPKKTYIVHMDKSSMPLDYDDHLQWYDSSIKSVS 1496
MK W +SI LV + AEE + K TYIVHMDKS+MP +DDH QWYDSS+K+ S
Sbjct: 1 MKTWRLSIVVLVSFHFALVVAEEIKHQQKNTYIVHMDKSNMPTTFDDHFQWYDSSLKTAS 60
Query: 1495 NSANMLYTYNNVIHGFSTRLTAAEAESLKGQPGILLVQEEMIYQLHTTRTPEFLGLDMST 1316
+SA+MLYTYNNV+HGFSTRLT EAE L+GQ GIL V E Y+LHTTRTPEFLGL S
Sbjct: 61 SSADMLYTYNNVVHGFSTRLTTEEAELLRGQLGILSVLPEARYELHTTRTPEFLGLGKSV 120
Query: 1315 AVYLEAGAVSEVIVGVLDTGVWPESRSFDDTGLGPIPSRWKGVCEVSKSFSISSCNKKLI 1136
A +A + SEVIVGVLDTGVWPE +SFDDTGLGP+PS WKG CE K+F +SSCN+KLI
Sbjct: 121 AFLPQADSASEVIVGVLDTGVWPELKSFDDTGLGPVPSSWKGECETGKTFPLSSCNRKLI 180
Query: 1135 GARSFSEGYEAAFGPVDETMESKSPRXXXXXXXXXXXXXXXXAVTRASLFNYAMGTARGM 956
GAR FS GYE AFGPV+ET+ES+SPR AV ASLF +A GTARGM
Sbjct: 181 GARFFSRGYEVAFGPVNETIESRSPRDDDGHGSHTSTTAVGSAVEGASLFGFAAGTARGM 240
Query: 955 ATLARVAAYKVCWIGGCFSSDILAGLEMAXXXXXXXXXXXXXXXXXDYFQDTVAVGAFSA 776
AT ARVAAYKVCW+GGC+ SDI+A ++ A DY +D+VA+GAF A
Sbjct: 241 ATHARVAAYKVCWLGGCYGSDIVAAMDKAVQDGVDVLSMSIGGGLSDYTKDSVAIGAFRA 300
Query: 775 MSHGIFVSCXXXXXXXXXXXXXNVAPWITTVGAGTLDRNFPANAILGNGKKFSGVSLYGG 596
M GI VSC NVAPWITTVGAGTLDR+FPA +LG+GKKFSGVSLY G
Sbjct: 301 MEQGILVSCSAGNGGPAPSSLSNVAPWITTVGAGTLDRDFPAFVMLGDGKKFSGVSLYSG 360
Query: 595 KPLSDSLVPLVYATNTS----GSICTTGSLDHEKVAGKIVVCDRGVNSRAQKGVVVRDAG 428
KPLSDSL+PLVYA N S G++C +L KVAGKIV+CDRG N+R QKG+VV++AG
Sbjct: 361 KPLSDSLIPLVYAGNASSSPNGNLCIPDNLIPGKVAGKIVLCDRGSNARVQKGIVVKEAG 420
Query: 427 GVGMILANTDSYGEELVADAHLIPTAAVGQTAGDAIKKYISSDPKPMATILFGGTQLGIQ 248
GVGMIL NTD YGEELVADAHL+PTAAVGQ AGD+IK YISSDP PMATI GGTQ+G+Q
Sbjct: 421 GVGMILTNTDLYGEELVADAHLLPTAAVGQKAGDSIKSYISSDPNPMATIAPGGTQVGVQ 480
Query: 247 PSPVVAAFSSRGP 209
PSPVVA+FSSRGP
Sbjct: 481 PSPVVASFSSRGP 493
Score = 81.3 bits (199), Expect(2) = 0.0
Identities = 37/46 (80%), Positives = 40/46 (86%)
Frame = -1
Query: 140 PITPEILKPDFIAPGVNIIAGWTGKVGPTALKDDTRHVDFNIISGT 3
P+TPEILKPD IAPGVNI+AGWTG VGPT L+ DTR V FNIISGT
Sbjct: 495 PVTPEILKPDIIAPGVNILAGWTGAVGPTGLQVDTRKVSFNIISGT 540
>ref|XP_002301156.1| predicted protein [Populus trichocarpa] gi|222842882|gb|EEE80429.1|
predicted protein [Populus trichocarpa]
Length = 779
Score = 605 bits (1561), Expect(2) = 0.0
Identities = 320/500 (64%), Positives = 370/500 (74%), Gaps = 9/500 (1%)
Frame = -2
Query: 1681 VKINTMKLWHISIATLVICCYGY----AEERTMPKKTYIVHMDKSSMPLDYDDHLQWYDS 1514
+K+ +K IATL++ C+ Y AE + KKT+I+ MDKS+MP +Y DH QWYDS
Sbjct: 8 MKMKDLKFGPQLIATLLVLCFCYTYAVAEVKKQTKKTFIIQMDKSNMPANYYDHFQWYDS 67
Query: 1513 SIKSVSNSANMLYTYNNVIHGFSTRLTAAEAESLKGQPGILLVQEEMIYQLHTTRTPEFL 1334
S+KSVS SA+MLYTYNN+IHGFST+LT EAE L+ Q GIL V EMIY+LHTT TPEFL
Sbjct: 68 SLKSVSESADMLYTYNNIIHGFSTQLTPDEAELLEKQSGILSVLPEMIYKLHTTHTPEFL 127
Query: 1333 GLDMSTAVYLEAGA-VSEVIVGVLDTGVWPESRSFDDTGLGPIPSRWKGVCEVSKSFSIS 1157
GL S AV L A A +SEVIVGVLDTGVWPE +SF DTGLGPIPS WKG C+V K+F+ S
Sbjct: 128 GLGKSDAVLLPASASLSEVIVGVLDTGVWPEIKSFGDTGLGPIPSTWKGSCQVGKNFNSS 187
Query: 1156 SCNKKLIGARSFSEGYEAAFGPVDETMESKSPRXXXXXXXXXXXXXXXXAVTRASLFNYA 977
SCN+KLIGA+ FS+GYEAAFGP+DETMESKSPR AV+ ASLF YA
Sbjct: 188 SCNRKLIGAQYFSKGYEAAFGPIDETMESKSPRDDDGHGTHTATTAAGSAVSGASLFGYA 247
Query: 976 MGTARGMATLARVAAYKVCWIGGCFSSDILAGLEMAXXXXXXXXXXXXXXXXXDYFQDTV 797
G ARGMAT ARVAAYKVCW+GGCFSSDILA +E A DY +DTV
Sbjct: 248 SGIARGMATEARVAAYKVCWLGGCFSSDILAAMEKAVADGVNVMSMSIGGGLSDYTRDTV 307
Query: 796 AVGAFSAMSHGIFVSCXXXXXXXXXXXXXNVAPWITTVGAGTLDRNFPANAILGNGKKFS 617
A+GAF A + GI VSC NVAPWITTVGAGTLDR+FPA LG+GKK+S
Sbjct: 308 AIGAFRAAAQGILVSCSAGNGGPSPGSLSNVAPWITTVGAGTLDRDFPAFVSLGDGKKYS 367
Query: 616 GVSLYGGKPLSDSLVPLVYATN----TSGSICTTGSLDHEKVAGKIVVCDRGVNSRAQKG 449
G+SLY GKPLSDSLVPLVYA N TSGS+C TG+L +VAGKIV+CDRG NSR QKG
Sbjct: 368 GISLYSGKPLSDSLVPLVYAGNVSNSTSGSLCMTGTLIPAQVAGKIVICDRGGNSRVQKG 427
Query: 448 VVVRDAGGVGMILANTDSYGEELVADAHLIPTAAVGQTAGDAIKKYISSDPKPMATILFG 269
+VV+D+GG+GMILANT+ YGEELVADAHL+PTAAVG +AIK Y DPKPM TI G
Sbjct: 428 LVVKDSGGLGMILANTELYGEELVADAHLLPTAAVGLRTANAIKNYAFLDPKPMGTIASG 487
Query: 268 GTQLGIQPSPVVAAFSSRGP 209
GT+LG++PSPVVAAFSSRGP
Sbjct: 488 GTKLGVEPSPVVAAFSSRGP 507
Score = 79.0 bits (193), Expect(2) = 0.0
Identities = 34/45 (75%), Positives = 39/45 (86%)
Frame = -1
Query: 137 ITPEILKPDFIAPGVNIIAGWTGKVGPTALKDDTRHVDFNIISGT 3
+TPE+LKPD IAPGVNI+AGWTG GPT L +D RHV+FNIISGT
Sbjct: 510 VTPEVLKPDLIAPGVNILAGWTGGAGPTGLTNDKRHVEFNIISGT 554
>ref|XP_002510119.1| Xylem serine proteinase 1 precursor, putative [Ricinus communis]
gi|223550820|gb|EEF52306.1| Xylem serine proteinase 1
precursor, putative [Ricinus communis]
Length = 769
Score = 598 bits (1541), Expect(2) = 0.0
Identities = 307/486 (63%), Positives = 361/486 (74%), Gaps = 7/486 (1%)
Frame = -2
Query: 1645 IATLVICCYGYA---EERTMPKKTYIVHMDKSSMPLDYDDHLQWYDSSIKSVSNSANMLY 1475
+A L+ CY + + + K TYI+HMDKS MP +DDHLQWYDSS+KSVS SA+MLY
Sbjct: 12 VAALLCFCYMHVIAGVKSSQSKNTYIIHMDKSYMPASFDDHLQWYDSSLKSVSESADMLY 71
Query: 1474 TYNNVIHGFSTRLTAAEAESLKGQPGILLVQEEMIYQLHTTRTPEFLGLDMSTAVYLEAG 1295
YNNVIHGFSTRLT+ EAE L+ Q GI+ V EMIY+LHTTRTPEFLGL S A + +
Sbjct: 72 DYNNVIHGFSTRLTSEEAELLEKQEGIISVLPEMIYELHTTRTPEFLGLGKSEAFFPTSD 131
Query: 1294 AVSEVIVGVLDTGVWPESRSFDDTGLGPIPSRWKGVCEVSKSFSISSCNKKLIGARSFSE 1115
+VSEV+VGVLDTGVWPE++SFDDTGLGPIP WKG CE K+F+ SSCN+KLIGAR FS+
Sbjct: 132 SVSEVVVGVLDTGVWPEAKSFDDTGLGPIPRTWKGECETGKNFNSSSCNRKLIGARFFSK 191
Query: 1114 GYEAAFGPVDETMESKSPRXXXXXXXXXXXXXXXXAVTRASLFNYAMGTARGMATLARVA 935
GYEAAFGPVDET+ES+SPR AV+ ASLF +A G ARGMAT ARVA
Sbjct: 192 GYEAAFGPVDETVESRSPRDDDGHGTHTSTTAAGSAVSGASLFGFATGIARGMATQARVA 251
Query: 934 AYKVCWIGGCFSSDILAGLEMAXXXXXXXXXXXXXXXXXDYFQDTVAVGAFSAMSHGIFV 755
AYKVCW+GGCF SDI+A ++ A DY++D VA+GAF+A + GI V
Sbjct: 252 AYKVCWLGGCFGSDIVAAMDKAVEDGVNVISMSIGGGLSDYYRDIVAIGAFTATAQGILV 311
Query: 754 SCXXXXXXXXXXXXXNVAPWITTVGAGTLDRNFPANAILGNGKKFSGVSLYGGKPLSDSL 575
SC N+APWITTVGAGTLDR+FPA LGNGK FSG SLY GKPLSDSL
Sbjct: 312 SCSAGNGGPSQGSLSNIAPWITTVGAGTLDRDFPAYVRLGNGKNFSGASLYSGKPLSDSL 371
Query: 574 VPLVYATN----TSGSICTTGSLDHEKVAGKIVVCDRGVNSRAQKGVVVRDAGGVGMILA 407
VPLV A N TSGS+C +G+L KVAGKIV+CDRG NSR QKG+ V++AGG+GMILA
Sbjct: 372 VPLVSAGNASNATSGSLCMSGTLIPTKVAGKIVICDRGGNSRVQKGLEVKNAGGIGMILA 431
Query: 406 NTDSYGEELVADAHLIPTAAVGQTAGDAIKKYISSDPKPMATILFGGTQLGIQPSPVVAA 227
NT+ YG+ELVADAHL+PTAAVGQT+ D IK+Y SD KP ATI FGGT +G++PSPVVAA
Sbjct: 432 NTELYGDELVADAHLLPTAAVGQTSADVIKRYAFSDLKPTATIAFGGTHIGVEPSPVVAA 491
Query: 226 FSSRGP 209
FSSRGP
Sbjct: 492 FSSRGP 497
Score = 80.1 bits (196), Expect(2) = 0.0
Identities = 36/45 (80%), Positives = 38/45 (84%)
Frame = -1
Query: 137 ITPEILKPDFIAPGVNIIAGWTGKVGPTALKDDTRHVDFNIISGT 3
+TPEILKPD IAPGVNI+AGWTG GPT L DDTR V FNIISGT
Sbjct: 500 VTPEILKPDIIAPGVNILAGWTGAAGPTGLTDDTRRVSFNIISGT 544
>ref|XP_004142884.1| PREDICTED: subtilisin-like protease-like isoform 1 [Cucumis sativus]
gi|449530704|ref|XP_004172333.1| PREDICTED:
subtilisin-like protease-like isoform 1 [Cucumis sativus]
Length = 770
Score = 578 bits (1489), Expect(2) = e-178
Identities = 296/479 (61%), Positives = 355/479 (74%), Gaps = 6/479 (1%)
Frame = -2
Query: 1627 CCYGYAEE--RTMPKKTYIVHMDKSSMPLDYDDHLQWYDSSIKSVSNSANMLYTYNNVIH 1454
C + A++ + + KKTYI+HMDK++MP +DDH QWYDSS+KSVS+SA MLY+YN VIH
Sbjct: 20 CSFTEAQKSNQQLKKKTYIIHMDKTNMPQAFDDHFQWYDSSLKSVSDSAQMLYSYNTVIH 79
Query: 1453 GFSTRLTAAEAESLKGQPGILLVQEEMIYQLHTTRTPEFLGLDMSTAVYLEAGAVSEVIV 1274
GFSTRLT EA+ ++ Q GI+ V EM Y+LHTTRTPEFLGL S + + + VSEVI+
Sbjct: 80 GFSTRLTVEEAKLMEKQEGIIAVIPEMKYELHTTRTPEFLGLGKSVSFFPASEKVSEVII 139
Query: 1273 GVLDTGVWPESRSFDDTGLGPIPSRWKGVCEVSKSFSISSCNKKLIGARSFSEGYEAAFG 1094
GVLDTGVWPE SF D GLGPIP+ WKG CEV K+F+ S+CN+KLIGAR FS+GYEAAFG
Sbjct: 140 GVLDTGVWPELESFSDAGLGPIPASWKGECEVGKNFTSSNCNRKLIGARYFSKGYEAAFG 199
Query: 1093 PVDETMESKSPRXXXXXXXXXXXXXXXXAVTRASLFNYAMGTARGMATLARVAAYKVCWI 914
P+DE+ ESKSPR AVT A+LF +A GTARGMA ARVA YKVCW+
Sbjct: 200 PIDESQESKSPRDDDGHGSHTSTTAAGSAVTGANLFGFAAGTARGMAAEARVATYKVCWL 259
Query: 913 GGCFSSDILAGLEMAXXXXXXXXXXXXXXXXXDYFQDTVAVGAFSAMSHGIFVSCXXXXX 734
GGCFSSDILA ++ + DY++D VA+GAFSA + G+FVSC
Sbjct: 260 GGCFSSDILAAMDKSVEDGCNILSVSLGGNSADYYRDNVAIGAFSATAQGVFVSCSAGNG 319
Query: 733 XXXXXXXXNVAPWITTVGAGTLDRNFPANAILGNGKKFSGVSLYGGKPLSDSLVPLVYA- 557
NVAPWITTVGAGTLDR+FPA LGNGKK +G SLY GKPL +SL+P+V A
Sbjct: 320 GPSSSTLSNVAPWITTVGAGTLDRDFPAYVTLGNGKKITGESLYSGKPLPNSLLPIVSAA 379
Query: 556 ---TNTSGSICTTGSLDHEKVAGKIVVCDRGVNSRAQKGVVVRDAGGVGMILANTDSYGE 386
++SGS+C +G+L+ KV GKIVVCDRG NSR QKGVVV++AGG+GMILANT++YGE
Sbjct: 380 SASNSSSGSLCLSGTLNPAKVTGKIVVCDRGGNSRVQKGVVVKEAGGLGMILANTEAYGE 439
Query: 385 ELVADAHLIPTAAVGQTAGDAIKKYISSDPKPMATILFGGTQLGIQPSPVVAAFSSRGP 209
E +ADAHLIPTAAVGQ AGDAIK YISSD P ATI G T+LG+QPSPVVAAFSSRGP
Sbjct: 440 EQLADAHLIPTAAVGQKAGDAIKNYISSDSNPTATISTGTTRLGVQPSPVVAAFSSRGP 498
Score = 75.9 bits (185), Expect(2) = e-178
Identities = 34/45 (75%), Positives = 37/45 (82%)
Frame = -1
Query: 137 ITPEILKPDFIAPGVNIIAGWTGKVGPTALKDDTRHVDFNIISGT 3
+TP+ILKPD IAPGVNI+AGWTG GPT L D RHV FNIISGT
Sbjct: 501 LTPQILKPDLIAPGVNILAGWTGGAGPTGLDSDKRHVAFNIISGT 545