BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00006351
         (498 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAC53892.1| cytochrome P450 [Petunia x hybrida]                   186   2e-45
ref|XP_002309107.1| cytochrome P450 [Populus trichocarpa] gi|222...   174   9e-42
dbj|BAC53891.1| cytochrome P450 [Petunia x hybrida]                   167   8e-40
sp|P37122.1|C76A2_SOLME RecName: Full=Cytochrome P450 76A2; AltN...   166   2e-39
emb|CBI30225.3| unnamed protein product [Vitis vinifera]              166   2e-39

>dbj|BAC53892.1| cytochrome P450 [Petunia x hybrida]
          Length = 510

 Score =  186 bits (472), Expect = 2e-45
 Identities = 92/166 (55%), Positives = 121/166 (72%), Gaps = 1/166 (0%)
 Frame = -3

Query: 496 KRINETVVIRQRCVDDMLSWIEKEVEKGACG-GIEVKSFVFPAMFNLIGNLTLSRDLMDP 320
           KRINET  +RQ+C+D ML WI+KE +    G GIEV  F+F A FN++GNL LSRDL+DP
Sbjct: 140 KRINETAHLRQKCIDSMLQWIDKEAKSMKKGSGIEVARFIFLASFNMMGNLMLSRDLVDP 199

Query: 319 HSEMASEFYMALSGFAECVSCPNISDLFPLLRWLDLQGLRRRMDRYMGKTIDIVSGFVNE 140
            S+ ASEF+ A+ G  E    PNISD+FP LRWLD+QGLR++  R MGK I++ S FV E
Sbjct: 200 ESKKASEFFTAMEGLMEWSGQPNISDIFPCLRWLDIQGLRQKAGRDMGKAIEVASTFVKE 259

Query: 139 RVKQRQDKVEQTTEEKDFLDMLLDFEGKGKDEPTRLSHHQITIFLM 2
           R+K+     ++   +KDFL++LL+FEG GKDEP +LS HQI IF++
Sbjct: 260 RLKEH----KEGEYKKDFLEVLLEFEGSGKDEPAKLSEHQINIFIL 301


>ref|XP_002309107.1| cytochrome P450 [Populus trichocarpa] gi|222855083|gb|EEE92630.1|
           cytochrome P450 [Populus trichocarpa]
          Length = 508

 Score =  174 bits (440), Expect = 9e-42
 Identities = 89/166 (53%), Positives = 115/166 (69%), Gaps = 1/166 (0%)
 Frame = -3

Query: 496 KRINETVVIRQRCVDDMLSWIEKEVEK-GACGGIEVKSFVFPAMFNLIGNLTLSRDLMDP 320
           KRINET  IR++CVDDML WIE+E  K G   GI V  FVF   FN++GNL LSRDL+DP
Sbjct: 142 KRINETASIRRKCVDDMLQWIEEESCKVGKAAGIHVSRFVFLMTFNMLGNLMLSRDLLDP 201

Query: 319 HSEMASEFYMALSGFAECVSCPNISDLFPLLRWLDLQGLRRRMDRYMGKTIDIVSGFVNE 140
            S++ SEF+ A+ G  E     N++D FP LR LDLQGLR+ M+R +GK ++I S FV E
Sbjct: 202 ESKVGSEFFDAMMGLMEWSGHANLADFFPWLRRLDLQGLRKNMERDLGKAMEIASKFVKE 261

Query: 139 RVKQRQDKVEQTTEEKDFLDMLLDFEGKGKDEPTRLSHHQITIFLM 2
           RV   +DK+  +   KDFLD+LL+F G GKDEP +LS   + IF++
Sbjct: 262 RV---EDKIVTSDSRKDFLDVLLEFRGSGKDEPDKLSERDVNIFIL 304


>dbj|BAC53891.1| cytochrome P450 [Petunia x hybrida]
          Length = 507

 Score =  167 bits (423), Expect = 8e-40
 Identities = 87/165 (52%), Positives = 117/165 (70%), Gaps = 1/165 (0%)
 Frame = -3

Query: 493 RINETVVIRQRCVDDMLSWIEKEVEKGACGGIEVKSFVFPAMFNLIGNLTLSRDLM-DPH 317
           RIN +  IR++ VD M+ WIE   +K    GIE+  FVF A FN++GNL +S++L  DP 
Sbjct: 142 RINNSESIRRKSVDKMIQWIETHGKKEQGQGIEITRFVFLASFNMLGNLIMSKELAADPD 201

Query: 316 SEMASEFYMALSGFAECVSCPNISDLFPLLRWLDLQGLRRRMDRYMGKTIDIVSGFVNER 137
           S  ASEF+ A+ G  E    PNISD+FPLLRWLD+QGLRR+M R MGK  +I+S F+ ER
Sbjct: 202 STTASEFFDAMMGQVEWSGTPNISDVFPLLRWLDIQGLRRKMKRDMGKGKEILSTFIKER 261

Query: 136 VKQRQDKVEQTTEEKDFLDMLLDFEGKGKDEPTRLSHHQITIFLM 2
           +K++++   + T   DFLD+LL FEGKGKDEP +LS H+I IF++
Sbjct: 262 IKEQENGRAKGT---DFLDVLLAFEGKGKDEPAKLSEHEINIFIL 303


>sp|P37122.1|C76A2_SOLME RecName: Full=Cytochrome P450 76A2; AltName: Full=CYPLXXVIA2;
           AltName: Full=Cytochrome P-450EG7
           gi|415911|emb|CAA50648.1| P450 hydroxylase [Solanum
           melongena]
          Length = 505

 Score =  166 bits (420), Expect = 2e-39
 Identities = 85/166 (51%), Positives = 118/166 (71%), Gaps = 1/166 (0%)
 Frame = -3

Query: 496 KRINETVVIRQRCVDDMLSWIEKEVEKGACG-GIEVKSFVFPAMFNLIGNLTLSRDLMDP 320
           K+I+ET  +R++CVD+ML WIEKE      G GIEV  FVF A FN++GNL LS+DL D 
Sbjct: 142 KKISETEPVRRKCVDNMLKWIEKEANSAEKGSGIEVTRFVFLASFNMLGNLILSKDLADL 201

Query: 319 HSEMASEFYMALSGFAECVSCPNISDLFPLLRWLDLQGLRRRMDRYMGKTIDIVSGFVNE 140
            SE ASEF++A+    E     N+SD+FP L+  DLQ LR++M R MGK ++I+S F+ E
Sbjct: 202 ESEEASEFFIAMKRINEWSGIANVSDIFPFLKKFDLQSLRKKMARDMGKAVEIMSMFLKE 261

Query: 139 RVKQRQDKVEQTTEEKDFLDMLLDFEGKGKDEPTRLSHHQITIFLM 2
           R ++R+   + T + KDFLD+LL+F+G GKDEP +LS H+I IF++
Sbjct: 262 REEERK---KGTEKGKDFLDVLLEFQGTGKDEPAKLSEHEIKIFVL 304


>emb|CBI30225.3| unnamed protein product [Vitis vinifera]
          Length = 1065

 Score =  166 bits (419), Expect = 2e-39
 Identities = 87/166 (52%), Positives = 116/166 (69%), Gaps = 2/166 (1%)
 Frame = -3

Query: 496  KRINETVVIRQRCVDDMLSWIEKEVE--KGACGGIEVKSFVFPAMFNLIGNLTLSRDLMD 323
            KRINETV IR++CVDD+L WIE+E     G   G+E+  F F A FN+IGNL LSRDL+D
Sbjct: 671  KRINETVPIRRKCVDDLLLWIEEEARGMDGTATGLELGRFFFLATFNMIGNLMLSRDLLD 730

Query: 322  PHSEMASEFYMALSGFAECVSCPNISDLFPLLRWLDLQGLRRRMDRYMGKTIDIVSGFVN 143
            P S   SEF+ A+    E     N +D FP L+WLD QGL++RM+  +GK+I+I SGFV 
Sbjct: 731  PQSRKGSEFFTAMRISMESSGHTNFADFFPWLKWLDPQGLKKRMEVDLGKSIEIASGFVK 790

Query: 142  ERVKQRQDKVEQTTEEKDFLDMLLDFEGKGKDEPTRLSHHQITIFL 5
            ER+  RQ + E+ ++ KDFLD+LL+F+G GKDE T++S   I IF+
Sbjct: 791  ERM--RQGRAEE-SKRKDFLDVLLEFQGDGKDEATKISEKGINIFI 833



 Score =  117 bits (293), Expect = 1e-24
 Identities = 61/158 (38%), Positives = 90/158 (56%), Gaps = 2/158 (1%)
 Frame = -3

Query: 472 IRQRCVDDMLSWIEKEVEKGACG--GIEVKSFVFPAMFNLIGNLTLSRDLMDPHSEMASE 299
           +R  C+D M+ ++    E G  G   I+V  F+F   FNLIGNL  S+DL+DP SE  +E
Sbjct: 168 VRGGCIDRMVQFV---TEAGTSGTHAIDVGRFIFLMAFNLIGNLMFSKDLLDPKSERGAE 224

Query: 298 FYMALSGFAECVSCPNISDLFPLLRWLDLQGLRRRMDRYMGKTIDIVSGFVNERVKQRQD 119
           F+       E    PN++D  P+LRW D QG+RR+   ++ +   I  GF+ ER+ +   
Sbjct: 225 FFYHAGKVMELAGRPNVADFLPILRWFDPQGIRRKTQFHVERAFAIAGGFIKERM-ETMA 283

Query: 118 KVEQTTEEKDFLDMLLDFEGKGKDEPTRLSHHQITIFL 5
           K     + KDFLD+LL+F G G +EP+R S   I + +
Sbjct: 284 KGSGEAKSKDFLDVLLEFRGDGVEEPSRFSSRTINVIV 321