BLASTX nr result
ID: Bupleurum21_contig00006296
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00006296
(1194 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002510380.1| 2-hydroxyacid dehydrogenase, putative [Ricin... 327 1e-93
ref|XP_002282654.1| PREDICTED: uncharacterized protein At1g01500... 323 4e-93
ref|XP_002301869.1| predicted protein [Populus trichocarpa] gi|2... 312 4e-90
gb|AAM62602.1| unknown [Arabidopsis thaliana] 277 4e-79
ref|NP_565103.1| Erythronate-4-phosphate dehydrogenase family pr... 276 5e-79
>ref|XP_002510380.1| 2-hydroxyacid dehydrogenase, putative [Ricinus communis]
gi|223551081|gb|EEF52567.1| 2-hydroxyacid dehydrogenase,
putative [Ricinus communis]
Length = 310
Score = 327 bits (838), Expect(2) = 1e-93
Identities = 159/253 (62%), Positives = 200/253 (79%)
Frame = +3
Query: 180 MENSHETLSNGKSRDPSLQIIRHPSYQSCSKLSLPWLDIKVLYVRISNFMVDESTPECLT 359
ME S+E +NG++ + +LQIIR+ +Q C+K S PW D++V YVRISNF VD STPECLT
Sbjct: 1 MEGSYEASTNGETANSNLQIIRYSPFQPCAKFSSPWFDLRVFYVRISNFTVDASTPECLT 60
Query: 360 VNHIPLSADTILEVNGARCKIHSEGISCVLRRDRVDKKAEEATFVSTANVRVTGSVKFEV 539
VNHIPLS T+LE+NG R ++S+G+S +LRRDRVDKK+EEATFVST ++R TGSVKFEV
Sbjct: 61 VNHIPLSPHTLLEINGVRSSMYSDGVSSLLRRDRVDKKSEEATFVSTDSIRTTGSVKFEV 120
Query: 540 FERDNLVLSGVLEMSNRNRLSGESKSNARRWSMNCEPVMSACTGFLKGKQIVGSESVSPT 719
F+ D+LVLSGVLEMS+ N GE KSN +RWSMNCE ++A TGFLKGK I G+E PT
Sbjct: 121 FDSDDLVLSGVLEMSSSNGFIGELKSNGKRWSMNCESEITAGTGFLKGKHIAGTE--LPT 178
Query: 720 VEVYVAGSFSGTPIILTKTIQLSFRKKHHRNGMLGAIPEYDTDESQDNTTSELNQQIVDY 899
+EVYV G FSGTPIILTKT+QL +RKKH R GML AIPEY+T ESQ N + E + Q+ +Y
Sbjct: 179 IEVYVTGCFSGTPIILTKTLQLGYRKKHIRKGMLDAIPEYETTESQKNVSREHDLQVAEY 238
Query: 900 RTYKMGKEDDYSN 938
R+Y+ E++Y+N
Sbjct: 239 RSYEAENEEEYNN 251
Score = 44.3 bits (103), Expect(2) = 1e-93
Identities = 22/61 (36%), Positives = 31/61 (50%)
Frame = +2
Query: 1010 SSLYWRHGEEYIEGEDGELSWFNAXXXXXXXXXXXXXXXXXXXXXXXXXTYQTTSRTFKR 1189
+++YWR EY++GEDGELSWFNA TYQ+T+ F++
Sbjct: 250 NNMYWR--TEYMDGEDGELSWFNAGVRVGVGIGLGICLGIGIGVGLLVRTYQSTTGNFRK 307
Query: 1190 R 1192
R
Sbjct: 308 R 308
>ref|XP_002282654.1| PREDICTED: uncharacterized protein At1g01500-like [Vitis vinifera]
Length = 308
Score = 323 bits (828), Expect(2) = 4e-93
Identities = 161/251 (64%), Positives = 198/251 (78%)
Frame = +3
Query: 180 MENSHETLSNGKSRDPSLQIIRHPSYQSCSKLSLPWLDIKVLYVRISNFMVDESTPECLT 359
ME+S+E + N + +P LQIIR+ YQS S PW D++V YVRISNF VD+STPE LT
Sbjct: 1 MESSYEAVRNDEPANPGLQIIRYTPYQSWSS---PWFDLRVFYVRISNFEVDDSTPEYLT 57
Query: 360 VNHIPLSADTILEVNGARCKIHSEGISCVLRRDRVDKKAEEATFVSTANVRVTGSVKFEV 539
+NHIPLS DT+LEVNG R I S+G+S +LRRDRVDKK+EEATFVST ++R+TGSVKFEV
Sbjct: 58 LNHIPLSPDTLLEVNGTRSSIFSDGVSSLLRRDRVDKKSEEATFVSTDSIRLTGSVKFEV 117
Query: 540 FERDNLVLSGVLEMSNRNRLSGESKSNARRWSMNCEPVMSACTGFLKGKQIVGSESVSPT 719
F++++L+LSG LEMSN N GESK+NA++WSMNCE ++A T FLKGKQIVGSE PT
Sbjct: 118 FDKEDLILSGALEMSNSNGFIGESKTNAKQWSMNCESEITAGTAFLKGKQIVGSELPPPT 177
Query: 720 VEVYVAGSFSGTPIILTKTIQLSFRKKHHRNGMLGAIPEYDTDESQDNTTSELNQQIVDY 899
+EVYVAG FSGTPIILTKT+QLSFRKKH+R G L +IPEY+T E Q N S L+ Q+ +Y
Sbjct: 178 IEVYVAGCFSGTPIILTKTLQLSFRKKHNRKGALDSIPEYETTECQKNVPSSLDLQMSEY 237
Query: 900 RTYKMGKEDDY 932
YK E+DY
Sbjct: 238 TNYKPESEEDY 248
Score = 46.2 bits (108), Expect(2) = 4e-93
Identities = 24/58 (41%), Positives = 28/58 (48%)
Frame = +2
Query: 1016 LYWRHGEEYIEGEDGELSWFNAXXXXXXXXXXXXXXXXXXXXXXXXXTYQTTSRTFKR 1189
+YWR E++EGEDGELSWFNA TYQ T+R FKR
Sbjct: 249 MYWRR-TEFMEGEDGELSWFNAGVRVGVGIGLGICLGIGIGVGLLVRTYQATTRNFKR 305
>ref|XP_002301869.1| predicted protein [Populus trichocarpa] gi|222843595|gb|EEE81142.1|
predicted protein [Populus trichocarpa]
Length = 313
Score = 312 bits (799), Expect(2) = 4e-90
Identities = 150/253 (59%), Positives = 200/253 (79%)
Frame = +3
Query: 180 MENSHETLSNGKSRDPSLQIIRHPSYQSCSKLSLPWLDIKVLYVRISNFMVDESTPECLT 359
ME +E SNG++ +LQIIR+ ++ +K PW D++V YVRISNF VD+STP+ LT
Sbjct: 1 MEGPYEASSNGEAAKSNLQIIRYSPFKPWTKFVSPWFDLRVFYVRISNFQVDDSTPQFLT 60
Query: 360 VNHIPLSADTILEVNGARCKIHSEGISCVLRRDRVDKKAEEATFVSTANVRVTGSVKFEV 539
+NHIPLS DT+LE+NG+R ++S+G++ +LRRDRVD+K+EEATFVST ++R TGSV+FEV
Sbjct: 61 LNHIPLSPDTLLEINGSRTSMYSDGVASLLRRDRVDRKSEEATFVSTDSIRSTGSVEFEV 120
Query: 540 FERDNLVLSGVLEMSNRNRLSGESKSNARRWSMNCEPVMSACTGFLKGKQIVGSESVSPT 719
F+ +LVLSGVLEMS+ N +GESKS+ +RW MNC+ ++ TGFLKGK GSE PT
Sbjct: 121 FDGKDLVLSGVLEMSSSNGFTGESKSSVKRWRMNCDSHITIGTGFLKGKYNGGSELPPPT 180
Query: 720 VEVYVAGSFSGTPIILTKTIQLSFRKKHHRNGMLGAIPEYDTDESQDNTTSELNQQIVDY 899
+EVYV GSFSGTPIILTKT+QL++RKKH+R GML +IPEY+T ES N + + + Q+VDY
Sbjct: 181 IEVYVTGSFSGTPIILTKTLQLTYRKKHNRKGMLDSIPEYETAESTKNASPDHDLQVVDY 240
Query: 900 RTYKMGKEDDYSN 938
R+YK+ E+DYSN
Sbjct: 241 RSYKLENEEDYSN 253
Score = 47.4 bits (111), Expect(2) = 4e-90
Identities = 24/61 (39%), Positives = 30/61 (49%)
Frame = +2
Query: 1010 SSLYWRHGEEYIEGEDGELSWFNAXXXXXXXXXXXXXXXXXXXXXXXXXTYQTTSRTFKR 1189
S++YWR E Y++GEDGELSWFNA TY+ T+ FKR
Sbjct: 252 SNMYWRRNE-YMDGEDGELSWFNAGVRVGVGLGLGICLGIGVGVGLLVRTYKATTHNFKR 310
Query: 1190 R 1192
R
Sbjct: 311 R 311
>gb|AAM62602.1| unknown [Arabidopsis thaliana]
Length = 315
Score = 277 bits (708), Expect(2) = 4e-79
Identities = 145/260 (55%), Positives = 187/260 (71%), Gaps = 7/260 (2%)
Frame = +3
Query: 180 MENSHETLSNGKSRDPSLQIIRHPSYQSC-----SKLSLPWLDIKVLYVRISNFMVDEST 344
M +ET +NG++ Q+IR+ SY S LS P LD++V YVRISNF VD ST
Sbjct: 1 MVEPYETRNNGEAS----QMIRYQSYNHHNSRLPSSLSSPLLDLRVFYVRISNFKVDHST 56
Query: 345 PECLTVNHIPLSADTILEVNGARCKIHSEGISCVLRRDRVDKKAEEATFVSTANVRVTGS 524
PE LT+ HIPL D++LE+NG R ++SEG+S LRRDRVDKK+E ATF+ST N+R++GS
Sbjct: 57 PEVLTITHIPLDPDSLLEINGVRMSMYSEGVSSQLRRDRVDKKSEVATFISTDNIRLSGS 116
Query: 525 VKFEVFERDNLVLSGVLEMSNRNRLSGESKSNARRWSMNCEPVMSACTGFLKGKQIVGSE 704
VKFEV+++D LVLSG LEMS N +GESK + +RW+MNCE ++A +GFLK K I GSE
Sbjct: 117 VKFEVYDKDELVLSGTLEMSGSNGFTGESKHSVKRWNMNCEAEITAGSGFLKEKHIGGSE 176
Query: 705 --SVSPTVEVYVAGSFSGTPIILTKTIQLSFRKKHHRNGMLGAIPEYDTDESQDNTTSEL 878
S PT+EVYV G FSGTPIILTKT+QL FRKKH R L +IPEY+TDE +SEL
Sbjct: 177 LSSPLPTIEVYVTGCFSGTPIILTKTLQLGFRKKHGRVTALDSIPEYETDEPHKGNSSEL 236
Query: 879 NQQIVDYRTYKMGKEDDYSN 938
+ Q+ +Y +YK E ++S+
Sbjct: 237 DYQVTEYGSYKQEYEGEHSD 256
Score = 45.8 bits (107), Expect(2) = 4e-79
Identities = 23/61 (37%), Positives = 29/61 (47%)
Frame = +2
Query: 1010 SSLYWRHGEEYIEGEDGELSWFNAXXXXXXXXXXXXXXXXXXXXXXXXXTYQTTSRTFKR 1189
S +YW EY +GEDGE+SWFNA TYQ+T+R F+R
Sbjct: 255 SDMYWNR--EYADGEDGEMSWFNAGVRVGVGIGLGVCVGLGIGVGLLVRTYQSTTRNFRR 312
Query: 1190 R 1192
R
Sbjct: 313 R 313
>ref|NP_565103.1| Erythronate-4-phosphate dehydrogenase family protein [Arabidopsis
thaliana] gi|42572111|ref|NP_974146.1|
Erythronate-4-phosphate dehydrogenase family protein
[Arabidopsis thaliana]
gi|10092268|gb|AAG12681.1|AC025814_5 unknown protein;
48213-49974 [Arabidopsis thaliana]
gi|23297334|gb|AAN12944.1| unknown protein [Arabidopsis
thaliana] gi|222423754|dbj|BAH19843.1| AT1G75180
[Arabidopsis thaliana] gi|332197561|gb|AEE35682.1|
Erythronate-4-phosphate dehydrogenase family protein
[Arabidopsis thaliana] gi|332197563|gb|AEE35684.1|
Erythronate-4-phosphate dehydrogenase family protein
[Arabidopsis thaliana]
Length = 315
Score = 276 bits (707), Expect(2) = 5e-79
Identities = 145/260 (55%), Positives = 187/260 (71%), Gaps = 7/260 (2%)
Frame = +3
Query: 180 MENSHETLSNGKSRDPSLQIIRHPSYQSC-----SKLSLPWLDIKVLYVRISNFMVDEST 344
M +ET +NG++ Q+IR+ SY S LS P LD++V YVRISNF VD ST
Sbjct: 1 MVEPYETRNNGEAS----QMIRYQSYNHHNSRLPSSLSSPLLDLRVFYVRISNFKVDHST 56
Query: 345 PECLTVNHIPLSADTILEVNGARCKIHSEGISCVLRRDRVDKKAEEATFVSTANVRVTGS 524
PE LT+ HIPL D++LE+NG R ++SEG+S LRRDRVDKK+E ATF+ST N+R++GS
Sbjct: 57 PEVLTITHIPLDPDSLLEINGVRMSMYSEGVSSQLRRDRVDKKSEVATFISTDNIRLSGS 116
Query: 525 VKFEVFERDNLVLSGVLEMSNRNRLSGESKSNARRWSMNCEPVMSACTGFLKGKQIVGSE 704
VKFEV+++D LVLSG LEMS N +GESK + +RW+MNCE ++A +GFLK K I GSE
Sbjct: 117 VKFEVYDKDELVLSGTLEMSGSNGFTGESKHSVKRWNMNCEAEITAESGFLKEKHIGGSE 176
Query: 705 --SVSPTVEVYVAGSFSGTPIILTKTIQLSFRKKHHRNGMLGAIPEYDTDESQDNTTSEL 878
S PT+EVYV G FSGTPIILTKT+QL FRKKH R L +IPEY+TDE +SEL
Sbjct: 177 LSSPLPTIEVYVTGCFSGTPIILTKTLQLGFRKKHGRVTALDSIPEYETDEPHKGNSSEL 236
Query: 879 NQQIVDYRTYKMGKEDDYSN 938
+ Q+ +Y +YK E ++S+
Sbjct: 237 DYQVTEYGSYKQEYEGEHSD 256
Score = 45.8 bits (107), Expect(2) = 5e-79
Identities = 23/61 (37%), Positives = 29/61 (47%)
Frame = +2
Query: 1010 SSLYWRHGEEYIEGEDGELSWFNAXXXXXXXXXXXXXXXXXXXXXXXXXTYQTTSRTFKR 1189
S +YW EY +GEDGE+SWFNA TYQ+T+R F+R
Sbjct: 255 SDMYWNR--EYADGEDGEMSWFNAGVRVGVGIGLGVCVGLGIGVGLLVRTYQSTTRNFRR 312
Query: 1190 R 1192
R
Sbjct: 313 R 313