BLASTX nr result
ID: Bupleurum21_contig00006223
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00006223
(1654 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ABC67804.1| protein kinase SRK [Nicotiana tabacum] 544 e-163
ref|XP_002271196.2| PREDICTED: CBL-interacting serine/threonine-... 546 e-163
ref|XP_003633013.1| PREDICTED: CBL-interacting serine/threonine-... 546 e-163
emb|CAN63583.1| hypothetical protein VITISV_006470 [Vitis vinife... 546 e-162
ref|XP_004134657.1| PREDICTED: CBL-interacting serine/threonine-... 541 e-162
>gb|ABC67804.1| protein kinase SRK [Nicotiana tabacum]
Length = 479
Score = 544 bits (1401), Expect(2) = e-163
Identities = 287/421 (68%), Positives = 317/421 (75%), Gaps = 2/421 (0%)
Frame = +3
Query: 327 KGGLIHHIKREISILRRVRHPNIVQLFEVMATKSKIFFVMEYVKGGELFSKVAKGRLKEE 506
K GLI HIKREISILRRVRHPNIVQL+EVMATK+KI+FVMEYVKGGELF+KVAKGRLKEE
Sbjct: 75 KVGLIDHIKREISILRRVRHPNIVQLYEVMATKAKIYFVMEYVKGGELFNKVAKGRLKEE 134
Query: 507 IARKYFQQLISAVGFCHARGVFHRDLKPENILLDEEGDLKVSDFGLSAISEQIRGDGLFH 686
+ARKYFQQLISAV FCHARGV+HRDLKPENILLDE+G++KVSDFGLSAISEQI+ DGLFH
Sbjct: 135 VARKYFQQLISAVAFCHARGVYHRDLKPENILLDEDGNVKVSDFGLSAISEQIKQDGLFH 194
Query: 687 TFCGTPAYVAPEVLGRKGYDAAKVDLWSCGIILFVXXXXXXXXXXXXXXXXXXXXXXXXX 866
TFCGTPAYVAPEVL RKGYDAAKVD+W CG+ILFV
Sbjct: 195 TFCGTPAYVAPEVLARKGYDAAKVDIWPCGVILFVLMAGYLPFHDQNIMAMYKKIYKGEF 254
Query: 867 XXXXXXXXXXXXXXXXXXPRWFSPEXXXXXXXXXDTNPKTRITIPEIMNNRWFKKGFKHI 1046
PRWFSPE DTNP+TRITI E+MNNRWFKKGFKH+
Sbjct: 255 RC----------------PRWFSPELTKFLRQLLDTNPETRITIQEVMNNRWFKKGFKHV 298
Query: 1047 KFYVEDDKVCSVVEEDDGIXXXXXXXXXXXXXXXXMRKRQLRLPRPASLNAFDIISMSSG 1226
KFY+EDDK+ SV ++D MR++ LPRPASLNAFDIIS S G
Sbjct: 299 KFYIEDDKLRSVNDDDYDGGVDYSSDRSESESEMEMRRKPATLPRPASLNAFDIISFSRG 358
Query: 1227 FDLSGLFEERGEHSRFVSRAPVESIISKLEEIAKVVKFTVRKKDCSVSLEGSREGVKGPL 1406
FDLSGLFEE G+ +RFVS APV IISKLEEIAKVV F VRKKDC VSLEGS+EG KGPL
Sbjct: 359 FDLSGLFEEGGDGARFVSGAPVPKIISKLEEIAKVVSFAVRKKDCRVSLEGSKEGEKGPL 418
Query: 1407 TIAAEIFELTPSLRVIEVKKKAGDRGEYEEFVNRELRPGLQNLMLMES--AESSNLLSDT 1580
TIAAEIFELTPSLRV+EVKKKAGDR EYEEF ++EL+PGLQNL L +SS+L SDT
Sbjct: 419 TIAAEIFELTPSLRVVEVKKKAGDRVEYEEFYHKELKPGLQNLTLEVDGPVDSSHLPSDT 478
Query: 1581 E 1583
E
Sbjct: 479 E 479
Score = 58.9 bits (141), Expect(2) = e-163
Identities = 27/35 (77%), Positives = 32/35 (91%)
Frame = +1
Query: 124 KNTTRKESQGLLLGRFEIGKLLGHGTFAKVYVARN 228
K+ ++KE+QGLL GR+EIGKLLGHGTFAKVY ARN
Sbjct: 22 KSLSKKENQGLLSGRYEIGKLLGHGTFAKVYHARN 56
>ref|XP_002271196.2| PREDICTED: CBL-interacting serine/threonine-protein kinase 12 isoform
1 [Vitis vinifera] gi|359483759|ref|XP_003633012.1|
PREDICTED: CBL-interacting serine/threonine-protein
kinase 12 isoform 2 [Vitis vinifera]
Length = 502
Score = 546 bits (1408), Expect(2) = e-163
Identities = 287/415 (69%), Positives = 315/415 (75%), Gaps = 2/415 (0%)
Frame = +3
Query: 327 KGGLIHHIKREISILRRVRHPNIVQLFEVMATKSKIFFVMEYVKGGELFSKVAKGRLKEE 506
KGGLI HIKREISILRRVRHPNIVQLFEVMATK+KI+FVMEYV+GGELF+KVAKGRLKEE
Sbjct: 64 KGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFNKVAKGRLKEE 123
Query: 507 IARKYFQQLISAVGFCHARGVFHRDLKPENILLDEEGDLKVSDFGLSAISEQIRGDGLFH 686
+ARKYFQQLISAVGFCHARGV+HRDLKPEN+LLDE GDLKVSDFGLSA+S+Q+R DGLFH
Sbjct: 124 VARKYFQQLISAVGFCHARGVYHRDLKPENLLLDEAGDLKVSDFGLSAVSDQMRQDGLFH 183
Query: 687 TFCGTPAYVAPEVLGRKGYDAAKVDLWSCGIILFVXXXXXXXXXXXXXXXXXXXXXXXXX 866
TFCGTPAYVAPEVL RKGYDA+KVD+WSCG+ILFV
Sbjct: 184 TFCGTPAYVAPEVLARKGYDASKVDIWSCGVILFV----------------LMAGYLPFH 227
Query: 867 XXXXXXXXXXXXXXXXXXPRWFSPEXXXXXXXXXDTNPKTRITIPEIMNNRWFKKGFKHI 1046
PRWFSPE DTNP+TRITIPEIM NRWFKKGFKHI
Sbjct: 228 DQNIMAMYKKIYKGEFRCPRWFSPELARLLTRLLDTNPETRITIPEIMENRWFKKGFKHI 287
Query: 1047 KFYVEDD--KVCSVVEEDDGIXXXXXXXXXXXXXXXXMRKRQLRLPRPASLNAFDIISMS 1220
KFY+EDD ++C+ E +D + R+R LPRPASLNAFDIIS S
Sbjct: 288 KFYIEDDTLRLCNAEEAEDDVESLSDQSMSESESELETRRRLSTLPRPASLNAFDIISFS 347
Query: 1221 SGFDLSGLFEERGEHSRFVSRAPVESIISKLEEIAKVVKFTVRKKDCSVSLEGSREGVKG 1400
GFDLSGLFEE G +RFVS APV IISKLEEIAKVV FTVRKKDC VSLEGSREGVKG
Sbjct: 348 PGFDLSGLFEEGGGDARFVSGAPVSKIISKLEEIAKVVSFTVRKKDCRVSLEGSREGVKG 407
Query: 1401 PLTIAAEIFELTPSLRVIEVKKKAGDRGEYEEFVNRELRPGLQNLMLMESAESSN 1565
PLTIAAEIFELTPSL V+EVKKK GD+GEYEEF NREL+P LQNL + +SA SS+
Sbjct: 408 PLTIAAEIFELTPSLVVVEVKKKGGDKGEYEEFCNRELKPVLQNL-VDDSAASSH 461
Score = 55.5 bits (132), Expect(2) = e-163
Identities = 30/45 (66%), Positives = 32/45 (71%), Gaps = 4/45 (8%)
Frame = +1
Query: 106 MATAGGKNTTRK----ESQGLLLGRFEIGKLLGHGTFAKVYVARN 228
MA K T K E+Q LLLGR+EIGKLLGHGTFAKVY ARN
Sbjct: 1 MADRQSKENTSKRKNGENQSLLLGRYEIGKLLGHGTFAKVYHARN 45
>ref|XP_003633013.1| PREDICTED: CBL-interacting serine/threonine-protein kinase 12 isoform
3 [Vitis vinifera]
Length = 464
Score = 546 bits (1408), Expect(2) = e-163
Identities = 285/412 (69%), Positives = 310/412 (75%), Gaps = 2/412 (0%)
Frame = +3
Query: 327 KGGLIHHIKREISILRRVRHPNIVQLFEVMATKSKIFFVMEYVKGGELFSKVAKGRLKEE 506
KGGLI HIKREISILRRVRHPNIVQLFEVMATK+KI+FVMEYV+GGELF+KVAKGRLKEE
Sbjct: 64 KGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFNKVAKGRLKEE 123
Query: 507 IARKYFQQLISAVGFCHARGVFHRDLKPENILLDEEGDLKVSDFGLSAISEQIRGDGLFH 686
+ARKYFQQLISAVGFCHARGV+HRDLKPEN+LLDE GDLKVSDFGLSA+S+Q+R DGLFH
Sbjct: 124 VARKYFQQLISAVGFCHARGVYHRDLKPENLLLDEAGDLKVSDFGLSAVSDQMRQDGLFH 183
Query: 687 TFCGTPAYVAPEVLGRKGYDAAKVDLWSCGIILFVXXXXXXXXXXXXXXXXXXXXXXXXX 866
TFCGTPAYVAPEVL RKGYDA+KVD+WSCG+ILFV
Sbjct: 184 TFCGTPAYVAPEVLARKGYDASKVDIWSCGVILFV----------------LMAGYLPFH 227
Query: 867 XXXXXXXXXXXXXXXXXXPRWFSPEXXXXXXXXXDTNPKTRITIPEIMNNRWFKKGFKHI 1046
PRWFSPE DTNP+TRITIPEIM NRWFKKGFKHI
Sbjct: 228 DQNIMAMYKKIYKGEFRCPRWFSPELARLLTRLLDTNPETRITIPEIMENRWFKKGFKHI 287
Query: 1047 KFYVEDD--KVCSVVEEDDGIXXXXXXXXXXXXXXXXMRKRQLRLPRPASLNAFDIISMS 1220
KFY+EDD ++C+ E +D + R+R LPRPASLNAFDIIS S
Sbjct: 288 KFYIEDDTLRLCNAEEAEDDVESLSDQSMSESESELETRRRLSTLPRPASLNAFDIISFS 347
Query: 1221 SGFDLSGLFEERGEHSRFVSRAPVESIISKLEEIAKVVKFTVRKKDCSVSLEGSREGVKG 1400
GFDLSGLFEE G +RFVS APV IISKLEEIAKVV FTVRKKDC VSLEGSREGVKG
Sbjct: 348 PGFDLSGLFEEGGGDARFVSGAPVSKIISKLEEIAKVVSFTVRKKDCRVSLEGSREGVKG 407
Query: 1401 PLTIAAEIFELTPSLRVIEVKKKAGDRGEYEEFVNRELRPGLQNLMLMESAE 1556
PLTIAAEIFELTPSL V+EVKKK GD+GEYEEF NREL+P LQNL AE
Sbjct: 408 PLTIAAEIFELTPSLVVVEVKKKGGDKGEYEEFCNRELKPVLQNLKSKRHAE 459
Score = 55.5 bits (132), Expect(2) = e-163
Identities = 30/45 (66%), Positives = 32/45 (71%), Gaps = 4/45 (8%)
Frame = +1
Query: 106 MATAGGKNTTRK----ESQGLLLGRFEIGKLLGHGTFAKVYVARN 228
MA K T K E+Q LLLGR+EIGKLLGHGTFAKVY ARN
Sbjct: 1 MADRQSKENTSKRKNGENQSLLLGRYEIGKLLGHGTFAKVYHARN 45
>emb|CAN63583.1| hypothetical protein VITISV_006470 [Vitis vinifera]
gi|229609803|gb|ACQ83517.1| CBL-interacting protein
kinase 01 [Vitis vinifera]
Length = 464
Score = 546 bits (1407), Expect(2) = e-162
Identities = 285/412 (69%), Positives = 310/412 (75%), Gaps = 2/412 (0%)
Frame = +3
Query: 327 KGGLIHHIKREISILRRVRHPNIVQLFEVMATKSKIFFVMEYVKGGELFSKVAKGRLKEE 506
KGGLI HIKREISILRRVRHPNIVQLFEVMATK+KI+FVMEYV+GGELF+KVAKGRLKEE
Sbjct: 64 KGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFNKVAKGRLKEE 123
Query: 507 IARKYFQQLISAVGFCHARGVFHRDLKPENILLDEEGDLKVSDFGLSAISEQIRGDGLFH 686
+ARKYFQQLISAVGFCHARGV+HRDLKPEN+LLDE GDLKVSDFGLSA+S+Q+R DGLFH
Sbjct: 124 VARKYFQQLISAVGFCHARGVYHRDLKPENLLLDEAGDLKVSDFGLSAVSDQMRQDGLFH 183
Query: 687 TFCGTPAYVAPEVLGRKGYDAAKVDLWSCGIILFVXXXXXXXXXXXXXXXXXXXXXXXXX 866
TFCGTPAYVAPEVL RKGYDA+KVD+WSCG+ILFV
Sbjct: 184 TFCGTPAYVAPEVLARKGYDASKVDIWSCGVILFV----------------LMAGYLPFH 227
Query: 867 XXXXXXXXXXXXXXXXXXPRWFSPEXXXXXXXXXDTNPKTRITIPEIMNNRWFKKGFKHI 1046
PRWFSPE DTNP+TRITIPEIM NRWFKKGFKHI
Sbjct: 228 DQNIMAMYKKIYKGEFRCPRWFSPELARLLTRLLDTNPETRITIPEIMENRWFKKGFKHI 287
Query: 1047 KFYVEDD--KVCSVVEEDDGIXXXXXXXXXXXXXXXXMRKRQLRLPRPASLNAFDIISMS 1220
KFY+EDD ++C+ E +D + R+R LPRPASLNAFDIIS S
Sbjct: 288 KFYIEDDTXRLCNAEEAEDDVESLSDQSMSESESELETRRRLSTLPRPASLNAFDIISFS 347
Query: 1221 SGFDLSGLFEERGEHSRFVSRAPVESIISKLEEIAKVVKFTVRKKDCSVSLEGSREGVKG 1400
GFDLSGLFEE G +RFVS APV IISKLEEIAKVV FTVRKKDC VSLEGSREGVKG
Sbjct: 348 PGFDLSGLFEEGGGDARFVSGAPVSKIISKLEEIAKVVSFTVRKKDCRVSLEGSREGVKG 407
Query: 1401 PLTIAAEIFELTPSLRVIEVKKKAGDRGEYEEFVNRELRPGLQNLMLMESAE 1556
PLTIAAEIFELTPSL V+EVKKK GD+GEYEEF NREL+P LQNL AE
Sbjct: 408 PLTIAAEIFELTPSLVVVEVKKKGGDKGEYEEFCNRELKPVLQNLKNKRHAE 459
Score = 55.5 bits (132), Expect(2) = e-162
Identities = 30/45 (66%), Positives = 32/45 (71%), Gaps = 4/45 (8%)
Frame = +1
Query: 106 MATAGGKNTTRK----ESQGLLLGRFEIGKLLGHGTFAKVYVARN 228
MA K T K E+Q LLLGR+EIGKLLGHGTFAKVY ARN
Sbjct: 1 MADRQSKENTSKRKNGENQSLLLGRYEIGKLLGHGTFAKVYHARN 45
>ref|XP_004134657.1| PREDICTED: CBL-interacting serine/threonine-protein kinase 12-like
[Cucumis sativus] gi|449508592|ref|XP_004163356.1|
PREDICTED: CBL-interacting serine/threonine-protein
kinase 12-like [Cucumis sativus]
Length = 467
Score = 541 bits (1395), Expect(2) = e-162
Identities = 277/420 (65%), Positives = 315/420 (75%), Gaps = 1/420 (0%)
Frame = +3
Query: 327 KGGLIHHIKREISILRRVRHPNIVQLFEVMATKSKIFFVMEYVKGGELFSKVAKGRLKEE 506
KGGLI HIKREISILRRVRHPNIVQLFEVMATK+KI+FVMEYV+GGELF KV+KGRLKEE
Sbjct: 64 KGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRLKEE 123
Query: 507 IARKYFQQLISAVGFCHARGVFHRDLKPENILLDEEGDLKVSDFGLSAISEQIRGDGLFH 686
+ARKYFQQLISAV FCHARGV+HRDLKPEN+LLDE G+LKVSDFGLSA+S+QIR DGLFH
Sbjct: 124 VARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFH 183
Query: 687 TFCGTPAYVAPEVLGRKGYDAAKVDLWSCGIILFVXXXXXXXXXXXXXXXXXXXXXXXXX 866
TFCGTPAYVAPEVL RKGY+AAKVD+WSCG+ILFV
Sbjct: 184 TFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFV----------------LMAGYLPFH 227
Query: 867 XXXXXXXXXXXXXXXXXXPRWFSPEXXXXXXXXXDTNPKTRITIPEIMNNRWFKKGFKHI 1046
PRWFSPE DTNP+TR TIPEIM NRWFKKG+KHI
Sbjct: 228 DQNIMAMYKKIYKGEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHI 287
Query: 1047 KFYVEDDKVCSVVEEDDGIXXXXXXXXXXXXXXXXMRKRQL-RLPRPASLNAFDIISMSS 1223
KFY+EDDKVCS+ ++ D + +R++ LPRPASLNAFDIIS S
Sbjct: 288 KFYIEDDKVCSIEDDTDDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSP 347
Query: 1224 GFDLSGLFEERGEHSRFVSRAPVESIISKLEEIAKVVKFTVRKKDCSVSLEGSREGVKGP 1403
GFDLSGLFE+ GE +RFVS APV IISKLEEIAK+V FTVRKKDC VSLEGSREGVKGP
Sbjct: 348 GFDLSGLFEDGGEEARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGP 407
Query: 1404 LTIAAEIFELTPSLRVIEVKKKAGDRGEYEEFVNRELRPGLQNLMLMESAESSNLLSDTE 1583
LTIAAE+FELTP L ++EVK+K GD+ EYE+F N EL+P L NL + +S + S++ SDTE
Sbjct: 408 LTIAAEVFELTPKLVMVEVKRKGGDKEEYEQFCNNELKPALLNLKVEDSGDPSHIPSDTE 467
Score = 57.4 bits (137), Expect(2) = e-162
Identities = 27/33 (81%), Positives = 29/33 (87%)
Frame = +1
Query: 130 TTRKESQGLLLGRFEIGKLLGHGTFAKVYVARN 228
TTRK+ LLLGR+EIGKLLGHGTFAKVY ARN
Sbjct: 13 TTRKDMPALLLGRYEIGKLLGHGTFAKVYHARN 45