BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00006139
         (1991 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFK79031.1| cytochrome P450 CYP736A54 [Bupleurum chinense]         917   0.0  
ref|XP_002280620.1| PREDICTED: cytochrome P450 71A1 [Vitis vinif...   621   e-175
ref|XP_002310007.1| cytochrome P450 [Populus trichocarpa] gi|222...   607   e-171
ref|XP_002532045.1| flavonoid 3-hydroxylase, putative [Ricinus c...   603   e-170
ref|XP_002310016.1| cytochrome P450 [Populus trichocarpa] gi|222...   603   e-170

>gb|AFK79031.1| cytochrome P450 CYP736A54 [Bupleurum chinense]
          Length = 497

 Score =  917 bits (2370), Expect = 0.0
 Identities = 464/497 (93%), Positives = 466/497 (93%)
 Frame = -2

Query: 1663 MAWTWIXXXXXXXLAYLVQSWLKKKTQRXXXXXXXXXXXXGHLHMLGKNPHQDLQKLAEK 1484
            MAWTWI       LAYLVQSWLKKKTQR            GHLHMLGKNPHQDLQKLAEK
Sbjct: 1    MAWTWITLLTLVVLAYLVQSWLKKKTQRKLPPGPKGLPIIGHLHMLGKNPHQDLQKLAEK 60

Query: 1483 HGPIMSMRFGFVPNIIVSSPEAAKQFLKTHDLNFAGRPSLEAAKYISYEQRNLSFSTYGP 1304
            HGPIMSMRFGFVPNIIVSSPEAAKQFLKTHDLNFAGRPSLEAAKYISYEQRNLSFSTYGP
Sbjct: 61   HGPIMSMRFGFVPNIIVSSPEAAKQFLKTHDLNFAGRPSLEAAKYISYEQRNLSFSTYGP 120

Query: 1303 YWRNMRKLCTLELLSNLKINSFQAMRKKEIANVVDIIEQAAQERVAVDISKRISSMNSDI 1124
            YWRNMRKLCTLELLSNLKINSFQAMRKKEIANVVDIIEQAAQERVAVDIS+RISSMNSDI
Sbjct: 121  YWRNMRKLCTLELLSNLKINSFQAMRKKEIANVVDIIEQAAQERVAVDISQRISSMNSDI 180

Query: 1123 SCQMVFGKKFEDKEFDERGFKGVIQVGMQMAVAFNLGDYFPYLGALDLQGLNKQMKAIAK 944
            SCQMVFGKKFEDKEFDERGFKGVIQVGMQMAVAFNLGDYFPYLGALDLQGLNK+MKAIAK
Sbjct: 181  SCQMVFGKKFEDKEFDERGFKGVIQVGMQMAVAFNLGDYFPYLGALDLQGLNKRMKAIAK 240

Query: 943  VWDQFLEKILDDHDQSKEPGQTKDFVDTMLDIMKSGESEFEFDRSHVKATLMDMFAASAD 764
            VWDQFLEKILDDHDQSKEPGQTKDFVDTMLDIMKSGESEFEFDRSHVKATLMDMFAASAD
Sbjct: 241  VWDQFLEKILDDHDQSKEPGQTKDFVDTMLDIMKSGESEFEFDRSHVKATLMDMFAASAD 300

Query: 763  TSSTTIEWTLSELLRHPRVMNKVQKELEQVVGMNRMVEESDLESLEYLGMVIKETMRXXX 584
            TSSTTIEWTLSELLRHPRVMNKVQKELEQVVGMNRMVEESDLESLEYLGMVIKETMR   
Sbjct: 301  TSSTTIEWTLSELLRHPRVMNKVQKELEQVVGMNRMVEESDLESLEYLGMVIKETMRLHP 360

Query: 583  XXXXXXXXXAIEDCTVDGFFIPKNSRVVVNVWAIGRDSNVWSDAEKFLPERFIGSNIDLR 404
                     AIEDCTVDGFFIPKNSRVVVNVWAIGRDSNVWSDAEKFLPERFIGSNIDLR
Sbjct: 361  VAPLLLPHLAIEDCTVDGFFIPKNSRVVVNVWAIGRDSNVWSDAEKFLPERFIGSNIDLR 420

Query: 403  GRDFELLPFGSGRRGCPGMQLGLTVVRLVVAQLLHCFDWDLPNGMQPSELDMTEEFGLLV 224
            GRDFELLPFGSGRRGCPGMQLGLTVVRLVVAQLLHCFDWDLPNGMQPSELDMTEEFGLLV
Sbjct: 421  GRDFELLPFGSGRRGCPGMQLGLTVVRLVVAQLLHCFDWDLPNGMQPSELDMTEEFGLLV 480

Query: 223  GRAKHLMAIPTCRLQRK 173
            GRAKHLMAIPTCRLQRK
Sbjct: 481  GRAKHLMAIPTCRLQRK 497


>ref|XP_002280620.1| PREDICTED: cytochrome P450 71A1 [Vitis vinifera]
          Length = 498

 Score =  621 bits (1601), Expect = e-175
 Identities = 306/497 (61%), Positives = 392/497 (78%), Gaps = 3/497 (0%)
 Frame = -2

Query: 1663 MAWTWIXXXXXXXLAYLVQSWLK--KKTQRXXXXXXXXXXXXGHLHMLGKNPHQDLQKLA 1490
            M W+WI        AY+V++ L   K   +            G+LHMLG+ PHQDL +LA
Sbjct: 1    MDWSWIALSLIAL-AYVVRALLNISKNKHKRLPPGPRGIPILGNLHMLGELPHQDLLRLA 59

Query: 1489 EKHGPIMSMRFGFVPNIIVSSPEAAKQFLKTHDLNFAGRPSLEAAKYISYEQRNLSFSTY 1310
            +K+GPIM MRF  VP I+VSSP+AA+QFLKT+DL FAGRP  E ++ +SY+++ +SF+ Y
Sbjct: 60   KKYGPIMYMRFALVPTIVVSSPQAAEQFLKTNDLVFAGRPPHEGSRIVSYDRKGISFTDY 119

Query: 1309 GPYWRNMRKLCTLELLSNLKINSFQAMRKKEIANVVDIIEQAAQERVAVDISKRISSMNS 1130
            GPYWRNMRKLCTL LLSNL+I+SFQ +R++E+  ++  +++AA  R AVD+S +ISS+++
Sbjct: 120  GPYWRNMRKLCTLGLLSNLRISSFQPLRREELDLLIKSLKEAALARTAVDLSAKISSLSA 179

Query: 1129 DISCQMVFGKKFEDKEFDERGFKGVIQVGMQMAVAFNLGDYFPYLGALDLQGLNKQMKAI 950
            D+SC+M+FGKK+ DK+ DERGFK VIQ GMQ+A A N+GDY P++  LDLQGL ++MKAI
Sbjct: 180  DMSCRMIFGKKYMDKDIDERGFKAVIQEGMQLAGAPNIGDYIPFVAPLDLQGLARRMKAI 239

Query: 949  AKVWDQFLEKILDDH-DQSKEPGQTKDFVDTMLDIMKSGESEFEFDRSHVKATLMDMFAA 773
            +KV+D F EKI+DDH  + KE GQ KD +D ML  M S E+EF+ +RS++KA ++DM A 
Sbjct: 240  SKVFDAFFEKIIDDHIHEPKEEGQPKDLIDVMLGYMGSKENEFQIERSNIKALVLDMLAG 299

Query: 772  SADTSSTTIEWTLSELLRHPRVMNKVQKELEQVVGMNRMVEESDLESLEYLGMVIKETMR 593
            S DTS+T IEW L+ELL++PR+M KVQ+ELE+VVGM R VEESDLESLEYL MV+KET+R
Sbjct: 300  SMDTSATAIEWALAELLKNPRIMKKVQEELEKVVGMERKVEESDLESLEYLDMVVKETLR 359

Query: 592  XXXXXXXXXXXXAIEDCTVDGFFIPKNSRVVVNVWAIGRDSNVWSDAEKFLPERFIGSNI 413
                        ++EDCTV+GF IP+ SRV+VN +AIGRD NVW+DAEKFLPERFIGS+I
Sbjct: 360  LHPVAPLLIPHESLEDCTVNGFHIPQKSRVMVNTYAIGRDPNVWTDAEKFLPERFIGSSI 419

Query: 412  DLRGRDFELLPFGSGRRGCPGMQLGLTVVRLVVAQLLHCFDWDLPNGMQPSELDMTEEFG 233
            DLRGRDF+L+PFGSGRRGCPGMQLGLTVVRLV+AQL+HCFDW+LPNGM PSELDMTEEFG
Sbjct: 420  DLRGRDFQLIPFGSGRRGCPGMQLGLTVVRLVLAQLVHCFDWELPNGMMPSELDMTEEFG 479

Query: 232  LLVGRAKHLMAIPTCRL 182
            L V RAKH++A+PT RL
Sbjct: 480  LTVPRAKHILAVPTYRL 496


>ref|XP_002310007.1| cytochrome P450 [Populus trichocarpa] gi|222852910|gb|EEE90457.1|
            cytochrome P450 [Populus trichocarpa]
          Length = 497

 Score =  607 bits (1564), Expect = e-171
 Identities = 295/495 (59%), Positives = 374/495 (75%), Gaps = 2/495 (0%)
 Frame = -2

Query: 1663 MAWTWIXXXXXXXLAYLVQSWLKKKTQRXXXXXXXXXXXXGH--LHMLGKNPHQDLQKLA 1490
            MAW  +       LA+ +++WL K+  +                LH+LGK PHQDL +LA
Sbjct: 1    MAWI-LTTLALIALAFFLRAWLSKRKIKDSKLPPGPIGFPIFGSLHLLGKFPHQDLHQLA 59

Query: 1489 EKHGPIMSMRFGFVPNIIVSSPEAAKQFLKTHDLNFAGRPSLEAAKYISYEQRNLSFSTY 1310
             K+GPIM MR G VP ++VSSP AA+  LKTHDL FA RP  EAAK+ISYEQ++LSF+ Y
Sbjct: 60   NKYGPIMYMRLGLVPTVVVSSPRAAELILKTHDLVFANRPPNEAAKHISYEQKSLSFAPY 119

Query: 1309 GPYWRNMRKLCTLELLSNLKINSFQAMRKKEIANVVDIIEQAAQERVAVDISKRISSMNS 1130
            G YWRN+RK+CTLELLSN KINSF + RK+E+  ++D I+ A++ERVAVD+S ++SS+++
Sbjct: 120  GSYWRNVRKMCTLELLSNHKINSFMSSRKEELDLLIDYIKDASRERVAVDLSAKVSSLSA 179

Query: 1129 DISCQMVFGKKFEDKEFDERGFKGVIQVGMQMAVAFNLGDYFPYLGALDLQGLNKQMKAI 950
            DISC+MVFGKK+ +KEFD++GFK VI  GM++A +FN GDY P +  LDLQGL K+MKA+
Sbjct: 180  DISCRMVFGKKYMEKEFDDKGFKPVIHEGMRLAASFNFGDYIPPIAPLDLQGLTKRMKAV 239

Query: 949  AKVWDQFLEKILDDHDQSKEPGQTKDFVDTMLDIMKSGESEFEFDRSHVKATLMDMFAAS 770
             KV+D FLEKI+D+H Q K+  +TKDFVD MLD + S E+E+   R ++KA ++DM   S
Sbjct: 240  GKVFDDFLEKIIDEHIQFKDENRTKDFVDVMLDFLGSEETEYSIGRDNIKAIILDMLVGS 299

Query: 769  ADTSSTTIEWTLSELLRHPRVMNKVQKELEQVVGMNRMVEESDLESLEYLGMVIKETMRX 590
             DTS+T IEWTLSEL++HPRVM KVQKELE+ +GM+RMVEESDLE LEYL MVIKE  R 
Sbjct: 300  MDTSATAIEWTLSELIKHPRVMKKVQKELEEKIGMDRMVEESDLEGLEYLHMVIKEAFRL 359

Query: 589  XXXXXXXXXXXAIEDCTVDGFFIPKNSRVVVNVWAIGRDSNVWSDAEKFLPERFIGSNID 410
                       ++EDCT+DGF IP+ +RV+VNVWAIGRD + W+DA KF+PERF GSNID
Sbjct: 360  HPVAPLLIPHESMEDCTIDGFLIPQKTRVIVNVWAIGRDQSAWTDANKFIPERFAGSNID 419

Query: 409  LRGRDFELLPFGSGRRGCPGMQLGLTVVRLVVAQLLHCFDWDLPNGMQPSELDMTEEFGL 230
            +RGRDF+LLPFG+GRRGCPGM LGLT+V  +VAQL+HCFDW+LPN M P ELDMTE FGL
Sbjct: 420  VRGRDFQLLPFGAGRRGCPGMHLGLTMVLQIVAQLVHCFDWELPNNMLPEELDMTEAFGL 479

Query: 229  LVGRAKHLMAIPTCR 185
            +  RA HL A PT R
Sbjct: 480  VTPRANHLCATPTYR 494


>ref|XP_002532045.1| flavonoid 3-hydroxylase, putative [Ricinus communis]
            gi|223528288|gb|EEF30335.1| flavonoid 3-hydroxylase,
            putative [Ricinus communis]
          Length = 505

 Score =  603 bits (1556), Expect = e-170
 Identities = 293/495 (59%), Positives = 374/495 (75%), Gaps = 1/495 (0%)
 Frame = -2

Query: 1663 MAWTWIXXXXXXXLAYL-VQSWLKKKTQRXXXXXXXXXXXXGHLHMLGKNPHQDLQKLAE 1487
            M+ TW          +L    WL+K   +            G LH+LGK PH++L +LA+
Sbjct: 9    MSLTWATLALIALACFLQALLWLRKTNNKKLPPGPRGFPIFGSLHLLGKYPHRELHRLAQ 68

Query: 1486 KHGPIMSMRFGFVPNIIVSSPEAAKQFLKTHDLNFAGRPSLEAAKYISYEQRNLSFSTYG 1307
            K+GPIM +R G V  I+VSSP+AA+ FLKTHDL FA RP  +AAK+ISYEQ+NLSF+ YG
Sbjct: 69   KYGPIMHLRLGLVSTIVVSSPQAAESFLKTHDLAFASRPPHQAAKFISYEQKNLSFAPYG 128

Query: 1306 PYWRNMRKLCTLELLSNLKINSFQAMRKKEIANVVDIIEQAAQERVAVDISKRISSMNSD 1127
             YWRN+RK+CTLELLSN+K+NSF++MRK+E+  ++D I+ A+ +RVAVD+S +++S+++D
Sbjct: 129  SYWRNVRKMCTLELLSNVKVNSFKSMRKEELDLLIDCIKNASCQRVAVDLSAKVASLSAD 188

Query: 1126 ISCQMVFGKKFEDKEFDERGFKGVIQVGMQMAVAFNLGDYFPYLGALDLQGLNKQMKAIA 947
            +SC+MVFGKK+ DKEFDERGFK VIQ GM +  A NLG+Y P +  LDLQGL K+MKA++
Sbjct: 189  MSCRMVFGKKYMDKEFDERGFKAVIQEGMHLGAAPNLGNYIPQIAGLDLQGLTKRMKAVS 248

Query: 946  KVWDQFLEKILDDHDQSKEPGQTKDFVDTMLDIMKSGESEFEFDRSHVKATLMDMFAASA 767
            KV+D F EKI+D+H + K+  +TKDFVD ML  M S  SE+   R ++KA ++DM A S 
Sbjct: 249  KVFDSFFEKIIDEHMEFKDENRTKDFVDVMLGFMGSEVSEYHIGRDNIKAIILDMLAGSM 308

Query: 766  DTSSTTIEWTLSELLRHPRVMNKVQKELEQVVGMNRMVEESDLESLEYLGMVIKETMRXX 587
            DTS+  IEW LSELL+HP VM KVQKELE+ VGM RMVEESD+E LEYL MVIKET R  
Sbjct: 309  DTSAAVIEWALSELLKHPGVMKKVQKELEEKVGMTRMVEESDVEKLEYLEMVIKETFRLH 368

Query: 586  XXXXXXXXXXAIEDCTVDGFFIPKNSRVVVNVWAIGRDSNVWSDAEKFLPERFIGSNIDL 407
                      A ED T+DG+ IPK S +++N +AIGRD + W++AEKFLPERF+G NID+
Sbjct: 369  PVAPLLLPHEAAEDTTIDGYLIPKKSHIIINTFAIGRDPSAWTEAEKFLPERFLGRNIDI 428

Query: 406  RGRDFELLPFGSGRRGCPGMQLGLTVVRLVVAQLLHCFDWDLPNGMQPSELDMTEEFGLL 227
            RGRDF+LLPFG+GRRGCPG+QLG+TVVRLVVAQL+HCFDW+LPNGM P+ELDMTEEF L+
Sbjct: 429  RGRDFQLLPFGAGRRGCPGIQLGMTVVRLVVAQLVHCFDWELPNGMLPTELDMTEEFSLV 488

Query: 226  VGRAKHLMAIPTCRL 182
              RAKHL AIP  RL
Sbjct: 489  TPRAKHLEAIPNYRL 503


>ref|XP_002310016.1| cytochrome P450 [Populus trichocarpa] gi|222852919|gb|EEE90466.1|
            cytochrome P450 [Populus trichocarpa]
          Length = 497

 Score =  603 bits (1555), Expect = e-170
 Identities = 295/496 (59%), Positives = 373/496 (75%), Gaps = 2/496 (0%)
 Frame = -2

Query: 1663 MAWTWIXXXXXXXLAYLVQSWLKKKTQRXXXXXXXXXXXXGH--LHMLGKNPHQDLQKLA 1490
            MAW  +       LA+ +++WL K+  +                LH+LGK PH DL +LA
Sbjct: 1    MAWI-LTTLALIALAFFLRAWLSKRKIKDSKLPPGPIGFPIFGSLHLLGKFPHHDLHQLA 59

Query: 1489 EKHGPIMSMRFGFVPNIIVSSPEAAKQFLKTHDLNFAGRPSLEAAKYISYEQRNLSFSTY 1310
            +K+GPIM MR G VP ++VSSP AA+  LKT+DL FA RP  EAAK+ISYEQ+NLSF+ Y
Sbjct: 60   KKYGPIMYMRLGLVPTVVVSSPRAAELILKTNDLVFASRPRNEAAKHISYEQKNLSFAPY 119

Query: 1309 GPYWRNMRKLCTLELLSNLKINSFQAMRKKEIANVVDIIEQAAQERVAVDISKRISSMNS 1130
            G YWRN+RK+CTLELLSN KINSF + RK+E+  ++D I+ A++ERVAVD+  ++SS+++
Sbjct: 120  GSYWRNVRKMCTLELLSNHKINSFMSTRKEELDLLIDYIKDASRERVAVDLGAKVSSLSA 179

Query: 1129 DISCQMVFGKKFEDKEFDERGFKGVIQVGMQMAVAFNLGDYFPYLGALDLQGLNKQMKAI 950
            DISC+MVFGKK+ +KEFDE+GFK V    M++A +FNLGDY P +  LDLQGL K+MKA+
Sbjct: 180  DISCRMVFGKKYLEKEFDEKGFKPVTHELMRLAASFNLGDYIPPIAPLDLQGLTKRMKAV 239

Query: 949  AKVWDQFLEKILDDHDQSKEPGQTKDFVDTMLDIMKSGESEFEFDRSHVKATLMDMFAAS 770
             KV+D F EKI+D+H Q K+  +TKDFVD MLD + S E+E+   R ++KA ++DM   S
Sbjct: 240  GKVFDDFFEKIIDEHIQFKDENRTKDFVDVMLDFLGSEETEYRIGRDNIKAIILDMLVGS 299

Query: 769  ADTSSTTIEWTLSELLRHPRVMNKVQKELEQVVGMNRMVEESDLESLEYLGMVIKETMRX 590
             DTS+T IEWTLSEL+RHPRVM KVQKELE+ +GM+RMVEESDLE LEYL MVIKE  R 
Sbjct: 300  MDTSATAIEWTLSELIRHPRVMKKVQKELEEKIGMDRMVEESDLEGLEYLHMVIKEAFRL 359

Query: 589  XXXXXXXXXXXAIEDCTVDGFFIPKNSRVVVNVWAIGRDSNVWSDAEKFLPERFIGSNID 410
                       ++EDCT+DGF IP+ +RV+VNVWAIGRD + W+DA KF+PERF GSNID
Sbjct: 360  HPVAPLLGPHESMEDCTIDGFLIPQKTRVIVNVWAIGRDQSAWTDANKFIPERFAGSNID 419

Query: 409  LRGRDFELLPFGSGRRGCPGMQLGLTVVRLVVAQLLHCFDWDLPNGMQPSELDMTEEFGL 230
            +RGRDF+LLPFG+GRRGCPGM LGLT+VR +VAQL+HCFDW+LPN M P ELDMTE FGL
Sbjct: 420  VRGRDFQLLPFGAGRRGCPGMHLGLTMVRQIVAQLVHCFDWELPNNMLPEELDMTEAFGL 479

Query: 229  LVGRAKHLMAIPTCRL 182
            +  RA HL A PT RL
Sbjct: 480  VTPRANHLCATPTYRL 495