BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00006122
         (1114 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI22425.3| unnamed protein product [Vitis vinifera]              418   e-114
ref|XP_002268333.1| PREDICTED: magnesium transporter MRS2-3-like...   418   e-114
ref|XP_004136933.1| PREDICTED: magnesium transporter MRS2-3-like...   383   e-104
ref|XP_002268374.1| PREDICTED: magnesium transporter MRS2-3-like...   382   e-103
ref|XP_002313918.1| magnesium transporter [Populus trichocarpa] ...   352   7e-95

>emb|CBI22425.3| unnamed protein product [Vitis vinifera]
          Length = 435

 Score =  418 bits (1075), Expect = e-114
 Identities = 226/348 (64%), Positives = 254/348 (72%), Gaps = 13/348 (3%)
 Frame = +1

Query: 1    DWTNLYDLEDSQSRPVSPPKLS---ECFPEKGEDSKQDGKQALENRDGLKLLPFEFIXXX 171
            DWTNLYDL + QSR VSP   S     F ++ E +K DG+  LEN+DGLK+LPFEFI   
Sbjct: 88   DWTNLYDLGEPQSRVVSPQNFSGDFSQFQDQDEGAKADGRPGLENQDGLKVLPFEFIALE 147

Query: 172  XXXXXXXSCLDNEARTLEQEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVRXX 351
                   SCL+NEARTLEQEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVR  
Sbjct: 148  ACLEAACSCLENEARTLEQEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVRDE 207

Query: 352  XXXXXXXXXXMAEMYLTDKLLQ-HLENSTVSSIDEQADIEDEVLRADAEDKIPDDIYPE- 525
                      MAEMYLTDK++Q HLENS+VSSI E+  ++D VL AD +D IPD+I  E 
Sbjct: 208  LEHLLDDDEDMAEMYLTDKMIQQHLENSSVSSIHERDGMDDGVLHADMDDGIPDEISLEA 267

Query: 526  --------GDLHHTDNQQDHLSNASNIHGRDSHGTRTNTTRSAISKHLDVEELEMLLEAY 681
                    GDL + DN Q+HL    +  GRDSHGT T+TT SAISKHLDVEELEMLLEAY
Sbjct: 268  NGVSATYEGDLQNIDNPQEHLFGTPHALGRDSHGTHTSTTHSAISKHLDVEELEMLLEAY 327

Query: 682  FVQIDGTLNKLSTLREYVDDTEDYINIMLDDKQNHLLQMGVMXXXXXXXXXXXXXXXXXX 861
            FVQIDGTLNKLSTLREYVDDTEDYINIMLDDKQNHLLQMGVM                  
Sbjct: 328  FVQIDGTLNKLSTLREYVDDTEDYINIMLDDKQNHLLQMGVMLTTATLVVSAFVVVAGIF 387

Query: 862  XMNITIDLFDEQKHGLKEFVWTICGSATGSIFLYVAAIGWCKHKRLLE 1005
             MNI I+LFD++K G++EF+WT+ G ATGSIFLYV AI WC+HKRLLE
Sbjct: 388  GMNIHIELFDDKKSGVQEFLWTVGGGATGSIFLYVIAIAWCRHKRLLE 435


>ref|XP_002268333.1| PREDICTED: magnesium transporter MRS2-3-like isoform 2 [Vitis
            vinifera]
          Length = 502

 Score =  418 bits (1075), Expect = e-114
 Identities = 226/348 (64%), Positives = 254/348 (72%), Gaps = 13/348 (3%)
 Frame = +1

Query: 1    DWTNLYDLEDSQSRPVSPPKLS---ECFPEKGEDSKQDGKQALENRDGLKLLPFEFIXXX 171
            DWTNLYDL + QSR VSP   S     F ++ E +K DG+  LEN+DGLK+LPFEFI   
Sbjct: 155  DWTNLYDLGEPQSRVVSPQNFSGDFSQFQDQDEGAKADGRPGLENQDGLKVLPFEFIALE 214

Query: 172  XXXXXXXSCLDNEARTLEQEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVRXX 351
                   SCL+NEARTLEQEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVR  
Sbjct: 215  ACLEAACSCLENEARTLEQEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVRDE 274

Query: 352  XXXXXXXXXXMAEMYLTDKLLQ-HLENSTVSSIDEQADIEDEVLRADAEDKIPDDIYPE- 525
                      MAEMYLTDK++Q HLENS+VSSI E+  ++D VL AD +D IPD+I  E 
Sbjct: 275  LEHLLDDDEDMAEMYLTDKMIQQHLENSSVSSIHERDGMDDGVLHADMDDGIPDEISLEA 334

Query: 526  --------GDLHHTDNQQDHLSNASNIHGRDSHGTRTNTTRSAISKHLDVEELEMLLEAY 681
                    GDL + DN Q+HL    +  GRDSHGT T+TT SAISKHLDVEELEMLLEAY
Sbjct: 335  NGVSATYEGDLQNIDNPQEHLFGTPHALGRDSHGTHTSTTHSAISKHLDVEELEMLLEAY 394

Query: 682  FVQIDGTLNKLSTLREYVDDTEDYINIMLDDKQNHLLQMGVMXXXXXXXXXXXXXXXXXX 861
            FVQIDGTLNKLSTLREYVDDTEDYINIMLDDKQNHLLQMGVM                  
Sbjct: 395  FVQIDGTLNKLSTLREYVDDTEDYINIMLDDKQNHLLQMGVMLTTATLVVSAFVVVAGIF 454

Query: 862  XMNITIDLFDEQKHGLKEFVWTICGSATGSIFLYVAAIGWCKHKRLLE 1005
             MNI I+LFD++K G++EF+WT+ G ATGSIFLYV AI WC+HKRLLE
Sbjct: 455  GMNIHIELFDDKKSGVQEFLWTVGGGATGSIFLYVIAIAWCRHKRLLE 502


>ref|XP_004136933.1| PREDICTED: magnesium transporter MRS2-3-like [Cucumis sativus]
            gi|449495702|ref|XP_004159919.1| PREDICTED: magnesium
            transporter MRS2-3-like [Cucumis sativus]
          Length = 494

 Score =  383 bits (984), Expect = e-104
 Identities = 209/342 (61%), Positives = 243/342 (71%), Gaps = 7/342 (2%)
 Frame = +1

Query: 1    DWTNLYDLEDSQSRPVSPPKLSECFPEKGEDSKQDGKQALENRDGLKLLPFEFIXXXXXX 180
            +W NLYDLE+ +SR  SPP        + E+ K+  KQ LENR+GLK+LPFEF+      
Sbjct: 154  NWRNLYDLEEPRSRTQSPPSYQGFPQAEEEEGKESMKQGLENREGLKVLPFEFVALEACL 213

Query: 181  XXXXSCLDNEARTLEQEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVRXXXXX 360
                SCL++EA TLE EAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVR     
Sbjct: 214  EAACSCLESEADTLELEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVRDELEH 273

Query: 361  XXXXXXXMAEMYLTDKLL-QHLENSTVSSIDEQADIEDEVLRADAEDKIPDDIYPEGD-- 531
                   MAEMYLT+K++ Q LENS+ SSI E+ D+E++  +   +D  P +I  +G   
Sbjct: 274  LLDDDEDMAEMYLTEKMVQQQLENSSTSSIPERDDMEEDDQQLGKDDSSPTEISMDGGGI 333

Query: 532  ----LHHTDNQQDHLSNASNIHGRDSHGTRTNTTRSAISKHLDVEELEMLLEAYFVQIDG 699
                  + D  QDHL  AS++ GRDSHGTRT+TT SAISKHLDVEELEMLLEAYFVQIDG
Sbjct: 334  SANYDGNMDASQDHLFGASHV-GRDSHGTRTSTTHSAISKHLDVEELEMLLEAYFVQIDG 392

Query: 700  TLNKLSTLREYVDDTEDYINIMLDDKQNHLLQMGVMXXXXXXXXXXXXXXXXXXXMNITI 879
            TLNKLSTLREYVDDTEDYINIMLDDKQNHLLQMGVM                   MNI I
Sbjct: 393  TLNKLSTLREYVDDTEDYINIMLDDKQNHLLQMGVMLTTATLVVSAFVVVAGIFGMNIHI 452

Query: 880  DLFDEQKHGLKEFVWTICGSATGSIFLYVAAIGWCKHKRLLE 1005
            +LF+ +K G+ EF+WT+ G ATG+IFLYV AI WCKHKRLLE
Sbjct: 453  ELFESEKAGMPEFLWTVGGGATGTIFLYVIAIAWCKHKRLLE 494


>ref|XP_002268374.1| PREDICTED: magnesium transporter MRS2-3-like isoform 3 [Vitis
            vinifera]
          Length = 463

 Score =  382 bits (980), Expect = e-103
 Identities = 211/339 (62%), Positives = 235/339 (69%), Gaps = 4/339 (1%)
 Frame = +1

Query: 1    DWTNLYDLEDSQSRPVSPPKLS---ECFPEKGEDSKQDGKQALENRDGLKLLPFEFIXXX 171
            DWTNLYDL + QSR VSP   S     F ++ E +K DG+  LEN+DGLK+LPFEFI   
Sbjct: 155  DWTNLYDLGEPQSRVVSPQNFSGDFSQFQDQDEGAKADGRPGLENQDGLKVLPFEFIALE 214

Query: 172  XXXXXXXSCLDNEARTLEQEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVRXX 351
                   SCL+NEARTLEQEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVR  
Sbjct: 215  ACLEAACSCLENEARTLEQEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVRDE 274

Query: 352  XXXXXXXXXXMAEMYLTDKLL-QHLENSTVSSIDEQADIEDEVLRADAEDKIPDDIYPEG 528
                      MAEMYLTDK++ QHLENS+VSSI E+  ++D VL AD +D          
Sbjct: 275  LEHLLDDDEDMAEMYLTDKMIQQHLENSSVSSIHERDGMDDGVLHADMDD---------- 324

Query: 529  DLHHTDNQQDHLSNASNIHGRDSHGTRTNTTRSAISKHLDVEELEMLLEAYFVQIDGTLN 708
                                 DSHGT T+TT SAISKHLDVEELEMLLEAYFVQIDGTLN
Sbjct: 325  --------------------GDSHGTHTSTTHSAISKHLDVEELEMLLEAYFVQIDGTLN 364

Query: 709  KLSTLREYVDDTEDYINIMLDDKQNHLLQMGVMXXXXXXXXXXXXXXXXXXXMNITIDLF 888
            KLSTLREYVDDTEDYINIMLDDKQNHLLQMGVM                   MNI I+LF
Sbjct: 365  KLSTLREYVDDTEDYINIMLDDKQNHLLQMGVMLTTATLVVSAFVVVAGIFGMNIHIELF 424

Query: 889  DEQKHGLKEFVWTICGSATGSIFLYVAAIGWCKHKRLLE 1005
            D++K G++EF+WT+ G ATGSIFLYV AI WC+HKRLLE
Sbjct: 425  DDKKSGVQEFLWTVGGGATGSIFLYVIAIAWCRHKRLLE 463


>ref|XP_002313918.1| magnesium transporter [Populus trichocarpa]
            gi|222850326|gb|EEE87873.1| magnesium transporter
            [Populus trichocarpa]
          Length = 462

 Score =  352 bits (904), Expect = 7e-95
 Identities = 196/331 (59%), Positives = 230/331 (69%), Gaps = 8/331 (2%)
 Frame = +1

Query: 37   SRPVSPPKLSECFPEKGEDSKQDGKQALENRDGLKLLPFEFIXXXXXXXXXXSCLDNEAR 216
            S P   P     F +  E  K +GKQ  ENRDG K+LPFEF+          SCL++EA+
Sbjct: 133  SSPEKSPVGFSHFEDCNEGIKAEGKQGFENRDGSKVLPFEFVALEACLEAACSCLESEAK 192

Query: 217  TLEQEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVRXXXXXXXXXXXXMAEMY 396
            TLEQEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVR            M E+Y
Sbjct: 193  TLEQEAHPALDKLTSKISTLNLERVRQIKSRLVAITGRVQKVRDEIEHLLDDDEDMVELY 252

Query: 397  LTDKLL-QHLENSTVSSIDEQADIEDEVLRADAEDKIPDDI------YPEGDLHHTDNQQ 555
            LT+KL+ Q LE+S+ SS++E  D++D+ L+AD++D IP ++        + D ++ DN+ 
Sbjct: 253  LTEKLVQQQLEDSSTSSLNEGNDMDDDELQADSDDSIPAEVSLGALASHKDDFNNIDNEH 312

Query: 556  DHLSNASNIHGRDSHGTRTNTTRSAISKHLDVEELEMLLEAYFVQIDGTLNKLSTLREYV 735
            DHL  A N  GR SH T T+TTRSAISKHL+VEELEMLLEAYFVQIDGTLNKLSTLREYV
Sbjct: 313  DHLFTAPNGLGRYSH-THTSTTRSAISKHLNVEELEMLLEAYFVQIDGTLNKLSTLREYV 371

Query: 736  DDTEDYINIMLDDKQNHLLQMGVMXXXXXXXXXXXXXXXXXXXMNITIDLFDEQ-KHGLK 912
            DDTEDYINIMLDDKQNHLLQMGVM                   MNI I LF E  + G+ 
Sbjct: 372  DDTEDYINIMLDDKQNHLLQMGVMLTTATLVVSCFVVVVGIFGMNIKIALFKEDVQTGMP 431

Query: 913  EFVWTICGSATGSIFLYVAAIGWCKHKRLLE 1005
            +F+WT+ G  +G IFLYV AI WCKHKRLLE
Sbjct: 432  KFLWTLAGGTSGVIFLYVIAIAWCKHKRLLE 462