BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00006103
         (1384 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFD33347.1| acyl-activating enzyme 3 [Cannabis sativa]             661   0.0  
ref|XP_002523698.1| AMP dependent CoA ligase, putative [Ricinus ...   659   0.0  
ref|XP_002276353.1| PREDICTED: 4-coumarate--CoA ligase-like 7 [V...   654   0.0  
ref|XP_002305282.1| 4-coumarate-coa ligase [Populus trichocarpa]...   649   0.0  
ref|XP_002329323.1| 4-coumarate-coa ligase [Populus trichocarpa]...   647   0.0  

>gb|AFD33347.1| acyl-activating enzyme 3 [Cannabis sativa]
          Length = 543

 Score =  661 bits (1706), Expect = 0.0
 Identities = 332/464 (71%), Positives = 394/464 (84%), Gaps = 4/464 (0%)
 Frame = -3

Query: 1382 KKNDVVLIFSPNSIQFPVCFFGIIAAGAVATTVNPSYTLSEVAKQVKDCNPKLIITVSGL 1203
            KKNDVVLI++PNSI FPVCF GIIA+GA+ATT NP YT+SE++KQVKD NPKLIITV  L
Sbjct: 77   KKNDVVLIYAPNSIHFPVCFLGIIASGAIATTSNPLYTVSELSKQVKDSNPKLIITVPQL 136

Query: 1202 FDKVKGFNLPVVILGDKENLKLFDSNLKVIYFDELVKNDGS----VLIQPRISVNDTAAL 1035
             +KVKGFNLP +++G     +   S+ KV+ F++LV   GS      I      +DTAAL
Sbjct: 137  LEKVKGFNLPTILIGPDSEQE--SSSDKVMTFNDLVNLGGSSGSEFPIVDDFKQSDTAAL 194

Query: 1034 LYSSGTTGVSKGVILTHKNFIAAALMVSSDLDLKGEMHSVYLCILPMFHVFGLTVITYCQ 855
            LYSSGTTG+SKGV+LTHKNFIA++LMV+ + DL GEM +V+LC LPMFHVFGL +ITY Q
Sbjct: 195  LYSSGTTGMSKGVVLTHKNFIASSLMVTMEQDLVGEMDNVFLCFLPMFHVFGLAIITYAQ 254

Query: 854  LQRGSTVVSMAKFDLEMLLRTVEKYKVTNMWVVPPIVLGLAKYNMINKYDLSSLKQIGSG 675
            LQRG+TV+SMA+FDLE +L+ VEKYKVT++WVVPP++L L+K +M+ K++LSS+K IGSG
Sbjct: 255  LQRGNTVISMARFDLEKMLKDVEKYKVTHLWVVPPVILALSKNSMVKKFNLSSIKYIGSG 314

Query: 674  AAPLGKELMQECAKKFPQALVMQGYGMTETTSIISVENQLGGPRHSGSAGMLVGGVECQI 495
            AAPLGK+LM+EC+K  P  +V QGYGMTET  I+S+E+  GG R+SGSAGML  GVE QI
Sbjct: 315  AAPLGKDLMEECSKVVPYGIVAQGYGMTETCGIVSMEDIRGGKRNSGSAGMLASGVEAQI 374

Query: 494  VSVDTLKRLPPKQLGEICVRGPNIMKGYYNNPQATKLTIDKQGWLHTGDLGYFDEEGQLY 315
            VSVDTLK LPP QLGEI V+GPN+M+GY+NNPQATKLTIDK+GW+HTGDLGYFDE+G LY
Sbjct: 375  VSVDTLKPLPPNQLGEIWVKGPNMMQGYFNNPQATKLTIDKKGWVHTGDLGYFDEDGHLY 434

Query: 314  VVDRLKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDAEAGEVPSAHVVRAPNSSL 135
            VVDR+KELIKYKGFQVAPAELEGLLVSHP+ILDAVVIPFPDAEAGEVP A+VVR+PNSSL
Sbjct: 435  VVDRIKELIKYKGFQVAPAELEGLLVSHPEILDAVVIPFPDAEAGEVPVAYVVRSPNSSL 494

Query: 134  TEEDVKNFIAEQVAPFKRLRRVTFITSVPKSASGKILRRELIEK 3
            TE DVK FIA QVA FKRLR+VTFI SVPKSASGKILRRELI+K
Sbjct: 495  TENDVKKFIAGQVASFKRLRKVTFINSVPKSASGKILRRELIQK 538


>ref|XP_002523698.1| AMP dependent CoA ligase, putative [Ricinus communis]
            gi|223537002|gb|EEF38638.1| AMP dependent CoA ligase,
            putative [Ricinus communis]
          Length = 542

 Score =  659 bits (1701), Expect = 0.0
 Identities = 325/461 (70%), Positives = 387/461 (83%), Gaps = 2/461 (0%)
 Frame = -3

Query: 1379 KNDVVLIFSPNSIQFPVCFFGIIAAGAVATTVNPSYTLSEVAKQVKDCNPKLIITVSGLF 1200
            KNDVVLIF+PNS QFP+CF  I + GA+ATT NP YT +E++KQ+KD NPKL+ITV  L+
Sbjct: 78   KNDVVLIFAPNSYQFPICFLAITSIGAIATTANPLYTTTEISKQIKDSNPKLVITVPELW 137

Query: 1199 DKVKGFNLPVVILGDKENLKLFDSNLKVIYFDELVKNDGSVLIQPRISVNDT--AALLYS 1026
            +KVK FNLP V LG KE+L L + N ++  FD LV+  GS    P I+V  T  A LLYS
Sbjct: 138  NKVKDFNLPAVFLGAKESL-LIEPNSRIKSFDHLVELGGSNSEFPTINVKQTDIATLLYS 196

Query: 1025 SGTTGVSKGVILTHKNFIAAALMVSSDLDLKGEMHSVYLCILPMFHVFGLTVITYCQLQR 846
            SGTTG+SKGVILTH NFIAA+ M++ D ++ GE+H+V+LC LPMFHVFGL VI Y QLQ 
Sbjct: 197  SGTTGISKGVILTHGNFIAASQMITMDQEIAGELHNVFLCFLPMFHVFGLAVIAYSQLQT 256

Query: 845  GSTVVSMAKFDLEMLLRTVEKYKVTNMWVVPPIVLGLAKYNMINKYDLSSLKQIGSGAAP 666
            G+ VVSM KFD E++L+ VEKY++T++WVVPP++L LAK +++ KYDLSSL+ +GSGAAP
Sbjct: 257  GNAVVSMGKFDFELVLKAVEKYRITHLWVVPPVILALAKQSLVKKYDLSSLQHVGSGAAP 316

Query: 665  LGKELMQECAKKFPQALVMQGYGMTETTSIISVENQLGGPRHSGSAGMLVGGVECQIVSV 486
            L KELM+ECAK  P A + QGYGMTETT I+SVEN   G RHSGSAG L  G+E QI+SV
Sbjct: 317  LSKELMEECAKTIPHAAIAQGYGMTETTGIVSVENPRIGVRHSGSAGTLAAGIEAQIISV 376

Query: 485  DTLKRLPPKQLGEICVRGPNIMKGYYNNPQATKLTIDKQGWLHTGDLGYFDEEGQLYVVD 306
            DTLK LPP QLGEI VRGPN+M+GY+NNPQATK TIDK+GWLHTGDLGYFDE+G+LYVVD
Sbjct: 377  DTLKPLPPNQLGEIWVRGPNMMRGYFNNPQATKQTIDKKGWLHTGDLGYFDEDGKLYVVD 436

Query: 305  RLKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDAEAGEVPSAHVVRAPNSSLTEE 126
            R+KELIKYKGFQVAPAELEGLLVSHP++LDAVVIPFPDAEAGEVP A+VVR+PNSSLTEE
Sbjct: 437  RIKELIKYKGFQVAPAELEGLLVSHPELLDAVVIPFPDAEAGEVPVAYVVRSPNSSLTEE 496

Query: 125  DVKNFIAEQVAPFKRLRRVTFITSVPKSASGKILRRELIEK 3
            +V+ +IA+QVAPFKRLRRVTFI +VPKSASGKILRRELIEK
Sbjct: 497  EVQKYIADQVAPFKRLRRVTFINTVPKSASGKILRRELIEK 537


>ref|XP_002276353.1| PREDICTED: 4-coumarate--CoA ligase-like 7 [Vitis vinifera]
          Length = 544

 Score =  654 bits (1687), Expect = 0.0
 Identities = 326/463 (70%), Positives = 385/463 (83%), Gaps = 3/463 (0%)
 Frame = -3

Query: 1382 KKNDVVLIFSPNSIQFPVCFFGIIAAGAVATTVNPSYTLSEVAKQVKDCNPKLIITVSGL 1203
            KKNDVVLIF+PNSIQ+P+CFFG+IA GA+ATT NP YT++E+ KQVKD N KL+IT+  L
Sbjct: 77   KKNDVVLIFAPNSIQYPLCFFGVIAIGAIATTANPLYTVAEIQKQVKDSNAKLVITIPQL 136

Query: 1202 FDKVKGFNLPVVILGDKENLK-LFDSNLKVIYFDELVKNDGSVLIQPRISVN--DTAALL 1032
            ++KVK  NLP VIL   +  K    S LK+ YF +LVK  G V   P++S+   DTAALL
Sbjct: 137  WEKVKALNLPAVILDSADTSKDTIQSPLKITYFSDLVKMAGEVSHFPKVSITQGDTAALL 196

Query: 1031 YSSGTTGVSKGVILTHKNFIAAALMVSSDLDLKGEMHSVYLCILPMFHVFGLTVITYCQL 852
            YSSGTTG SKGVILTH NF+AAALMV+ D +L GEMH+V+LC LPMFHVFGL V+   QL
Sbjct: 197  YSSGTTGASKGVILTHGNFVAAALMVTMDEELMGEMHNVFLCFLPMFHVFGLAVVMCAQL 256

Query: 851  QRGSTVVSMAKFDLEMLLRTVEKYKVTNMWVVPPIVLGLAKYNMINKYDLSSLKQIGSGA 672
            Q G T+VSM KFDL++ L+++EKY+VT+MW+VPP++L L K   +++YD+SSLK IGSGA
Sbjct: 257  QMGYTIVSMPKFDLDVALKSIEKYRVTHMWLVPPVMLALVKQGKLDRYDISSLKHIGSGA 316

Query: 671  APLGKELMQECAKKFPQALVMQGYGMTETTSIISVENQLGGPRHSGSAGMLVGGVECQIV 492
            APLGKELM+ECAK  P   V QGYGMTET  I+S E    G +H+GS G LV GVE QI+
Sbjct: 317  APLGKELMEECAKSLPHVAVGQGYGMTETCGIVSKEIPKIGIQHTGSTGPLVSGVEAQII 376

Query: 491  SVDTLKRLPPKQLGEICVRGPNIMKGYYNNPQATKLTIDKQGWLHTGDLGYFDEEGQLYV 312
            SVDTLK LPP QLGEI VRGPN+MKGY+NNPQATKLTIDK+GW+HTGD+GYFDE+G+L+V
Sbjct: 377  SVDTLKPLPPNQLGEIWVRGPNMMKGYFNNPQATKLTIDKKGWVHTGDVGYFDEQGKLFV 436

Query: 311  VDRLKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDAEAGEVPSAHVVRAPNSSLT 132
            VDR+KELIKYKGFQVAPAELE LLVSHP+ILDAVVIPFPDAEAGEVP A+VVR+PNSSLT
Sbjct: 437  VDRIKELIKYKGFQVAPAELEALLVSHPEILDAVVIPFPDAEAGEVPIAYVVRSPNSSLT 496

Query: 131  EEDVKNFIAEQVAPFKRLRRVTFITSVPKSASGKILRRELIEK 3
            EEDVK FIA QVAPFK+LRRV+FI +VPKSASGKILRRELIEK
Sbjct: 497  EEDVKTFIANQVAPFKKLRRVSFINTVPKSASGKILRRELIEK 539


>ref|XP_002305282.1| 4-coumarate-coa ligase [Populus trichocarpa]
            gi|222848246|gb|EEE85793.1| 4-coumarate-coa ligase
            [Populus trichocarpa]
          Length = 543

 Score =  649 bits (1673), Expect = 0.0
 Identities = 322/461 (69%), Positives = 383/461 (83%), Gaps = 2/461 (0%)
 Frame = -3

Query: 1379 KNDVVLIFSPNSIQFPVCFFGIIAAGAVATTVNPSYTLSEVAKQVKDCNPKLIITVSGLF 1200
            KNDVVLIF+PNS QFP+CF  I + GA+ATT NP YT SE++KQ+KD NPKLIITV  L+
Sbjct: 78   KNDVVLIFAPNSYQFPICFLAITSTGAIATTANPLYTTSELSKQIKDSNPKLIITVPELW 137

Query: 1199 DKVKGFNLPVVILGDKENLKLFDSNLKVIYFDELVKNDGSVLIQPRISV--NDTAALLYS 1026
            DKVKGFNLP V LG K      +S  ++  FD LV   GS    P  +V  +D + LLYS
Sbjct: 138  DKVKGFNLPAVFLGPKRVSLPLESGSRITSFDSLVGLGGSNSQFPSSNVKQSDISTLLYS 197

Query: 1025 SGTTGVSKGVILTHKNFIAAALMVSSDLDLKGEMHSVYLCILPMFHVFGLTVITYCQLQR 846
            SGTTGVSKGVILTH NFIAA+LMV+ D  + GEMH V+LC LPMFHVFGL VITY QLQ 
Sbjct: 198  SGTTGVSKGVILTHGNFIAASLMVTMDQAMAGEMHDVFLCFLPMFHVFGLAVITYSQLQM 257

Query: 845  GSTVVSMAKFDLEMLLRTVEKYKVTNMWVVPPIVLGLAKYNMINKYDLSSLKQIGSGAAP 666
            G+ VVSM KF+ EM+L+T+EK++VT+MWVVPP+VL LAK +M+ KYD+SSL+ IGSGAAP
Sbjct: 258  GNAVVSMGKFEFEMVLKTIEKHRVTDMWVVPPVVLALAKQDMVKKYDISSLRNIGSGAAP 317

Query: 665  LGKELMQECAKKFPQALVMQGYGMTETTSIISVENQLGGPRHSGSAGMLVGGVECQIVSV 486
            LGK+LM+ECAK  P  ++ QGYGMTET  I+SVE+   G RHSGSAG+L  G+E QIVSV
Sbjct: 318  LGKDLMKECAKNLPDTIISQGYGMTETCGIVSVEDSRLGVRHSGSAGILAAGIEAQIVSV 377

Query: 485  DTLKRLPPKQLGEICVRGPNIMKGYYNNPQATKLTIDKQGWLHTGDLGYFDEEGQLYVVD 306
            +TLK LPP QLGEI VRGPN+M+GY+NNPQATK T+DK+GW+HTGD+GYFD++GQL+VVD
Sbjct: 378  ETLKPLPPNQLGEIWVRGPNMMRGYFNNPQATKDTMDKKGWVHTGDVGYFDDDGQLFVVD 437

Query: 305  RLKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDAEAGEVPSAHVVRAPNSSLTEE 126
            R+KELIKYKGFQVAPAELEGLLVSHP ILDAVVIP+PDAEAGEVP A+VVR+PNS+LTE 
Sbjct: 438  RIKELIKYKGFQVAPAELEGLLVSHPQILDAVVIPYPDAEAGEVPVAYVVRSPNSALTEV 497

Query: 125  DVKNFIAEQVAPFKRLRRVTFITSVPKSASGKILRRELIEK 3
            DV+ FIA+QVAPFKRLR+VTFI +VPKSASGKILRRELI++
Sbjct: 498  DVQKFIADQVAPFKRLRKVTFINTVPKSASGKILRRELIQQ 538


>ref|XP_002329323.1| 4-coumarate-coa ligase [Populus trichocarpa]
            gi|224127626|ref|XP_002329324.1| acyl:coa ligase [Populus
            trichocarpa] gi|222870777|gb|EEF07908.1| 4-coumarate-coa
            ligase [Populus trichocarpa] gi|222870778|gb|EEF07909.1|
            acyl:coa ligase [Populus trichocarpa]
          Length = 543

 Score =  647 bits (1669), Expect = 0.0
 Identities = 321/461 (69%), Positives = 383/461 (83%), Gaps = 2/461 (0%)
 Frame = -3

Query: 1379 KNDVVLIFSPNSIQFPVCFFGIIAAGAVATTVNPSYTLSEVAKQVKDCNPKLIITVSGLF 1200
            KNDV+LIF+PNS QFP CF  I + GAVATT NP YT SE++KQ+KD NPKL+ITV  L+
Sbjct: 78   KNDVILIFAPNSYQFPTCFLAITSIGAVATTANPLYTTSELSKQIKDSNPKLVITVPELW 137

Query: 1199 DKVKGFNLPVVILGDKENLKLFDSNLKVIYFDELVKNDGSVLIQPRISV--NDTAALLYS 1026
            DKVKGFNLP V LG KE     +S  ++  F  LV+  GS    P   V  +D A LLYS
Sbjct: 138  DKVKGFNLPAVFLGPKEVSLPLESGSRIRSFHSLVELGGSNSEFPVSDVKQSDIATLLYS 197

Query: 1025 SGTTGVSKGVILTHKNFIAAALMVSSDLDLKGEMHSVYLCILPMFHVFGLTVITYCQLQR 846
            SGTTGVSKGVILTH NFIAA+LMVS D  + GE+H+V+LC LPMFHVFGL VITY QLQ 
Sbjct: 198  SGTTGVSKGVILTHGNFIAASLMVSMDQVMAGEIHNVFLCFLPMFHVFGLAVITYSQLQM 257

Query: 845  GSTVVSMAKFDLEMLLRTVEKYKVTNMWVVPPIVLGLAKYNMINKYDLSSLKQIGSGAAP 666
            G+ VVSM KF+ EM+LRT+EKY+VT+MWVVPP++L L+K N++ KYDLSSL+ IGSGAAP
Sbjct: 258  GNAVVSMGKFEFEMVLRTIEKYRVTHMWVVPPVILALSKQNLVKKYDLSSLRNIGSGAAP 317

Query: 665  LGKELMQECAKKFPQALVMQGYGMTETTSIISVENQLGGPRHSGSAGMLVGGVECQIVSV 486
            LGK+LM+ECAK  P   ++QG+GMTET  I+S+E+   G RHSGSAG+L  G+E QI+SV
Sbjct: 318  LGKDLMKECAKNLPDTTIIQGFGMTETCGIVSLEDPRIGVRHSGSAGILNAGIEAQIISV 377

Query: 485  DTLKRLPPKQLGEICVRGPNIMKGYYNNPQATKLTIDKQGWLHTGDLGYFDEEGQLYVVD 306
            +T K LPP QLGEI VRGPN+M+GY+NNPQATK TIDK+GW+HTGDLGYFD++GQL+VVD
Sbjct: 378  ETAKPLPPNQLGEIWVRGPNMMRGYFNNPQATKDTIDKKGWVHTGDLGYFDDDGQLFVVD 437

Query: 305  RLKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDAEAGEVPSAHVVRAPNSSLTEE 126
            R+KELIKYKGFQVAPAELEGLLVSHP+ILDAVV P+PDAEAGEVP A+VVR+PNS+LTEE
Sbjct: 438  RIKELIKYKGFQVAPAELEGLLVSHPEILDAVVTPYPDAEAGEVPVAYVVRSPNSALTEE 497

Query: 125  DVKNFIAEQVAPFKRLRRVTFITSVPKSASGKILRRELIEK 3
            DV+ FI++QVAPFKRLR+VTFI SVPKSASGKILRREL++K
Sbjct: 498  DVQKFISDQVAPFKRLRKVTFINSVPKSASGKILRRELVQK 538