BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00006053
         (1350 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002525989.1| Desacetoxyvindoline 4-hydroxylase, putative ...   319   1e-88
ref|XP_002284896.1| PREDICTED: 1-aminocyclopropane-1-carboxylate...   328   1e-87
gb|ABK96576.1| unknown [Populus trichocarpa x Populus deltoides]      305   1e-85
ref|XP_002315859.1| predicted protein [Populus trichocarpa] gi|2...   305   1e-85
ref|XP_003637672.1| Gibberellin 20 oxidase [Medicago truncatula]...   292   4e-81

>ref|XP_002525989.1| Desacetoxyvindoline 4-hydroxylase, putative [Ricinus communis]
           gi|223534721|gb|EEF36413.1| Desacetoxyvindoline
           4-hydroxylase, putative [Ricinus communis]
          Length = 361

 Score =  319 bits (817), Expect(2) = 1e-88
 Identities = 147/251 (58%), Positives = 193/251 (76%), Gaps = 1/251 (0%)
 Frame = -2

Query: 785 KLFNEQPIDVKSQYYHRDISRGASFSTNFDLLQSRAASWRDTLQVRLAPVSPDWNYVPEV 606
           K F+E P +VK ++Y R+   G +F +N DL ++RAASWRDTLQ+RL P  P+   +PE+
Sbjct: 111 KGFHELPTEVKQRWYQRETVTGVNFFSNVDLFKARAASWRDTLQIRLGPNLPEVEEIPEI 170

Query: 605 CRKEVAAWEKEMVKFGDXXXXXXXXXXXXEKNKLKDKTCLDGRMLVGHYYPYCPQPDLTV 426
           CR EV  W +  V+  +            +   LK+ TCL+ R++VGHYYP+CPQPDLTV
Sbjct: 171 CRNEVIEWSQLAVQVAELLMELLCEGLGLKSETLKEMTCLEARVMVGHYYPHCPQPDLTV 230

Query: 425 GITPHTDPGVVTVLVQNEVGGLQVKNGDDWVDVEPLPGALVINIGDILQIISNDEYKSVE 246
           GIT HTDPGV+T+L+Q+ VGGLQVK+GD+WVDV+P+PGALVINIGDILQI+SNDEY+SVE
Sbjct: 231 GITSHTDPGVLTLLLQDSVGGLQVKHGDEWVDVKPVPGALVINIGDILQIMSNDEYRSVE 290

Query: 245 HRVLANSLREPRISVAVFFNAGNREDMYGPLPEIVSPEKPPVYRQFQLADFMKRFLTKEL 66
           HRVLAN  R+PR+S+A+FFN G R+  YGP P+++S EKP VY+QF L DF+KRF +KEL
Sbjct: 291 HRVLANPSRDPRVSIAIFFNPGKRDCAYGPFPDLISAEKPAVYKQFMLMDFLKRFFSKEL 350

Query: 65  DGK-MTDYYRL 36
           DGK +T+YY+L
Sbjct: 351 DGKTLTNYYKL 361



 Score = 35.4 bits (80), Expect(2) = 1e-88
 Identities = 14/28 (50%), Positives = 20/28 (71%)
 Frame = -1

Query: 897 RPSAVVDQIRRASSSLGFFQVINHSVPI 814
           R S +V++I RA   LGFFQ++NH V +
Sbjct: 73  RRSVIVEEISRACRELGFFQIVNHGVHV 100


>ref|XP_002284896.1| PREDICTED: 1-aminocyclopropane-1-carboxylate oxidase homolog 4-like
           [Vitis vinifera]
          Length = 393

 Score =  328 bits (842), Expect = 1e-87
 Identities = 147/251 (58%), Positives = 196/251 (78%), Gaps = 1/251 (0%)
 Frame = -2

Query: 785 KLFNEQPIDVKSQYYHRDISRGASFSTNFDLLQSRAASWRDTLQVRLAPVSPDWNYVPEV 606
           + FNEQP +VK+QYY RDI  G +FSTNFDL  S+AASWRDTLQVRL P   +++ +PEV
Sbjct: 141 RAFNEQPTEVKAQYYRRDIGTGVAFSTNFDLYHSKAASWRDTLQVRLGPTPTEFDKMPEV 200

Query: 605 CRKEVAAWEKEMVKFGDXXXXXXXXXXXXEKNKLKDKTCLDGRMLVGHYYPYCPQPDLTV 426
           CR+EV  W++EMV+ G+            +  +LK+ TCL+GR++  HYYPYCPQP+LT+
Sbjct: 201 CRREVMEWDREMVRLGEALMGLMCEGLGLDAGRLKELTCLEGRIMASHYYPYCPQPELTM 260

Query: 425 GITPHTDPGVVTVLVQNEVGGLQVKNGDDWVDVEPLPGALVINIGDILQIISNDEYKSVE 246
           G+TPHTDPGV+TV++QN+VGGLQVK+   W+D+ P+PGALVIN+GD+LQ++SNDEYKSVE
Sbjct: 261 GLTPHTDPGVLTVVLQNQVGGLQVKHDGAWLDLNPVPGALVINVGDMLQVMSNDEYKSVE 320

Query: 245 HRVLANSLREPRISVAVFFNAGNREDMYGPLPEIVSPEKPPVYRQFQLADFMKRFLTKEL 66
           HRV  N  RE R+S+AVFFN G+R  ++GPLPE++S EKP VY+ F   +FM RF TKEL
Sbjct: 321 HRVAGNPCREARVSIAVFFNPGDRHSLFGPLPELISAEKPAVYKSFTFDEFMTRFFTKEL 380

Query: 65  DGK-MTDYYRL 36
           DGK + ++++L
Sbjct: 381 DGKSLINFFKL 391


>gb|ABK96576.1| unknown [Populus trichocarpa x Populus deltoides]
          Length = 408

 Score =  305 bits (780), Expect(2) = 1e-85
 Identities = 141/251 (56%), Positives = 184/251 (73%), Gaps = 1/251 (0%)
 Frame = -2

Query: 785 KLFNEQPIDVKSQYYHRDISRGASFSTNFDLLQSRAASWRDTLQVRLAPVSPDWNYVPEV 606
           K F+E P + K ++Y R++  G SF +N DL  S+AASWRDTLQ+ L P  P+   +PE+
Sbjct: 158 KGFHELPTEEKMRWYRREMGSGVSFLSNVDLFHSKAASWRDTLQMTLGPNLPELEEIPEI 217

Query: 605 CRKEVAAWEKEMVKFGDXXXXXXXXXXXXEKNKLKDKTCLDGRMLVGHYYPYCPQPDLTV 426
           CR E+  W +   + G+               KLKD T LD R +  HYYPYCPQPDLTV
Sbjct: 218 CRNELVDWNQCAKQLGEVLMELLCEGLGLNAGKLKDLTFLDARTMAAHYYPYCPQPDLTV 277

Query: 425 GITPHTDPGVVTVLVQNEVGGLQVKNGDDWVDVEPLPGALVINIGDILQIISNDEYKSVE 246
           GI  HTDPGV+TVL+Q+++GGLQVK+G+ WVDV+P+PGA+VIN+GDI+QI+SNDEYKS E
Sbjct: 278 GIMSHTDPGVLTVLLQDQIGGLQVKHGEGWVDVKPVPGAIVINVGDIMQILSNDEYKSNE 337

Query: 245 HRVLANSLREPRISVAVFFNAGNREDMYGPLPEIVSPEKPPVYRQFQLADFMKRFLTKEL 66
           HRVLAN   EPRIS+A+FFN   R+ ++GP PE++SPEKP VYR+F   D++KRF TKEL
Sbjct: 338 HRVLANGCHEPRISIAIFFNPLKRDSLFGPFPELISPEKPAVYREFIYTDYIKRFFTKEL 397

Query: 65  DGK-MTDYYRL 36
           DGK +T+YY+L
Sbjct: 398 DGKSLTNYYKL 408



 Score = 39.7 bits (91), Expect(2) = 1e-85
 Identities = 17/28 (60%), Positives = 21/28 (75%)
 Frame = -1

Query: 897 RPSAVVDQIRRASSSLGFFQVINHSVPI 814
           R SAVVD++ RA    GFFQV+NH VP+
Sbjct: 120 RRSAVVDKVGRACREFGFFQVVNHGVPL 147


>ref|XP_002315859.1| predicted protein [Populus trichocarpa] gi|222864899|gb|EEF02030.1|
           predicted protein [Populus trichocarpa]
          Length = 371

 Score =  305 bits (780), Expect(2) = 1e-85
 Identities = 141/251 (56%), Positives = 184/251 (73%), Gaps = 1/251 (0%)
 Frame = -2

Query: 785 KLFNEQPIDVKSQYYHRDISRGASFSTNFDLLQSRAASWRDTLQVRLAPVSPDWNYVPEV 606
           K F+E P + K ++Y R++  G SF +N DL  S+AASWRDTLQ+ L P  P+   +PE+
Sbjct: 121 KGFHELPTEEKMRWYRREMGSGVSFLSNVDLFHSKAASWRDTLQMTLGPNLPELEEIPEI 180

Query: 605 CRKEVAAWEKEMVKFGDXXXXXXXXXXXXEKNKLKDKTCLDGRMLVGHYYPYCPQPDLTV 426
           CR E+  W +   + G+               KLKD T LD R +  HYYPYCPQPDLTV
Sbjct: 181 CRNELVDWNQCAKQLGELLMELLCEGLGLNAGKLKDLTFLDARTMAAHYYPYCPQPDLTV 240

Query: 425 GITPHTDPGVVTVLVQNEVGGLQVKNGDDWVDVEPLPGALVINIGDILQIISNDEYKSVE 246
           GI  HTDPGV+TVL+Q+++GGLQVK+G+ WVDV+P+PGA+VIN+GDI+QI+SNDEYKS E
Sbjct: 241 GIMSHTDPGVLTVLLQDQIGGLQVKHGEGWVDVKPVPGAIVINVGDIMQILSNDEYKSNE 300

Query: 245 HRVLANSLREPRISVAVFFNAGNREDMYGPLPEIVSPEKPPVYRQFQLADFMKRFLTKEL 66
           HRVLAN   EPRIS+A+FFN   R+ ++GP PE++SPEKP VYR+F   D++KRF TKEL
Sbjct: 301 HRVLANGCHEPRISIAIFFNPLKRDSLFGPFPELISPEKPAVYREFIYTDYIKRFFTKEL 360

Query: 65  DGK-MTDYYRL 36
           DGK +T+YY+L
Sbjct: 361 DGKSLTNYYKL 371



 Score = 39.7 bits (91), Expect(2) = 1e-85
 Identities = 17/28 (60%), Positives = 21/28 (75%)
 Frame = -1

Query: 897 RPSAVVDQIRRASSSLGFFQVINHSVPI 814
           R SAVVD++ RA    GFFQV+NH VP+
Sbjct: 83  RRSAVVDKVGRACREFGFFQVVNHGVPL 110


>ref|XP_003637672.1| Gibberellin 20 oxidase [Medicago truncatula]
           gi|355503607|gb|AES84810.1| Gibberellin 20 oxidase
           [Medicago truncatula]
          Length = 365

 Score =  292 bits (747), Expect(2) = 4e-81
 Identities = 135/250 (54%), Positives = 180/250 (72%), Gaps = 1/250 (0%)
 Frame = -2

Query: 785 KLFNEQPIDVKSQYYHRDISRGASFSTNFDLLQSRAASWRDTLQVRLAPVSPDWNYVPEV 606
           K F+EQP + K + Y R++  G S+ +N DL  S+AASWRDTL++++ PV  D   +PEV
Sbjct: 115 KAFHEQPAEEKEKVYRREMETGVSYISNVDLFNSKAASWRDTLRIKMGPVPADEKEIPEV 174

Query: 605 CRKEVAAWEKEMVKFGDXXXXXXXXXXXXEKNKLKDKTCLDGRMLVGHYYPYCPQPDLTV 426
           CRKEV  W+KE+ + GD             + +L D     GR +VGHYYP+CPQPDLTV
Sbjct: 175 CRKEVKEWDKEVKRVGDILLGLLSEGLGLGEERLIDMGLSQGRGMVGHYYPFCPQPDLTV 234

Query: 425 GITPHTDPGVVTVLVQNEVGGLQVKNGDDWVDVEPLPGALVINIGDILQIISNDEYKSVE 246
           G+  HTDPG +TVL+Q+ +GGLQV+    W++V+PL GALVINIGD LQIISN+ YKSV+
Sbjct: 235 GLNSHTDPGALTVLLQDHIGGLQVRTQHGWINVKPLDGALVINIGDFLQIISNEVYKSVD 294

Query: 245 HRVLANSLREPRISVAVFFNAGNREDMYGPLPEIVSPEKPPVYRQFQLADFMKRFLTKEL 66
           HRVLAN   EPR+S+AVFFN GNRE ++GPLPE+ S EKP +Y+ F+L +F+ RF  KEL
Sbjct: 295 HRVLANPSNEPRVSIAVFFNPGNREKLFGPLPELTSAEKPALYKDFKLNEFLTRFFNKEL 354

Query: 65  DGK-MTDYYR 39
           DGK  T++++
Sbjct: 355 DGKSFTNFFK 364



 Score = 37.7 bits (86), Expect(2) = 4e-81
 Identities = 17/24 (70%), Positives = 21/24 (87%)
 Frame = -1

Query: 891 SAVVDQIRRASSSLGFFQVINHSV 820
           SAVV+QIR A+S+ GFF+VINH V
Sbjct: 79  SAVVEQIRSAASTFGFFRVINHGV 102