BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00005577
         (1855 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003635124.1| PREDICTED: asparaginyl-tRNA synthetase, chlo...   859   0.0  
ref|XP_002327980.1| predicted protein [Populus trichocarpa] gi|2...   846   0.0  
ref|XP_002309798.1| predicted protein [Populus trichocarpa] gi|2...   845   0.0  
ref|XP_004147209.1| PREDICTED: asparagine--tRNA ligase, chloropl...   818   0.0  
emb|CAA10904.1| asparaginyl-tRNA synthetase [Arabidopsis thaliana]    793   0.0  

>ref|XP_003635124.1| PREDICTED: asparaginyl-tRNA synthetase,
            chloroplastic/mitochondrial-like isoform 2 [Vitis
            vinifera] gi|297744376|emb|CBI37350.3| unnamed protein
            product [Vitis vinifera]
          Length = 584

 Score =  859 bits (2219), Expect = 0.0
 Identities = 422/515 (81%), Positives = 454/515 (88%), Gaps = 3/515 (0%)
 Frame = +2

Query: 17   RTFSGLIAAALSSGEAIEKQKAGVLESD---KVGKFRKRLRIVDIKGGPDEGLDKLGENI 187
            R F   I+ ALSSGEAIEK K     ++   KVG+FRKRLR+VDIKGGPDEGLD+LG+ +
Sbjct: 70   RCFCTAISGALSSGEAIEKPKPEFFSAETGEKVGEFRKRLRVVDIKGGPDEGLDRLGQTL 129

Query: 188  VVKGWVRTLRVQSSVTFIEVNDGSCLSNMQCVVSSDAQGYDQVENGLTTTGASICVEGIL 367
             VKGWVRTLRVQSSV FIEVNDGSCLSNMQCV++SDA GYDQVE+G  TTGAS+ ++G L
Sbjct: 130  AVKGWVRTLRVQSSVIFIEVNDGSCLSNMQCVMNSDADGYDQVESGSITTGASVSIQGTL 189

Query: 368  VTSQGSKQXXXXXXXXXXTVGKSDPSFPIQKKRVSREFLRTKSHLRPRTNTFGAVARVRN 547
            V SQGSKQ          TVGKSDPSFPIQKKRVSREFLRTK+HLRPRTNTFGAVARVRN
Sbjct: 190  VASQGSKQKVELKVLKIVTVGKSDPSFPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRN 249

Query: 548  ALAYATHKFFNENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSREAIESPADGLPKTKD 727
            ALAYATHKFF ENGFVW+SSPIITASDCEGAGEQFCVTTLIPSSREA +S  D +PK KD
Sbjct: 250  ALAYATHKFFQENGFVWISSPIITASDCEGAGEQFCVTTLIPSSREATDSTVDAIPKAKD 309

Query: 728  GLIDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEP 907
            GLIDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEP
Sbjct: 310  GLIDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEP 369

Query: 908  ELAFANLDDDMACATAYLQYVVKYVLENCKEDMDFFNSWIEKGIVSRLSDVVEKDFVQLT 1087
            ELAFA+L+DDMACATAYLQYVV Y+LENCKEDM+FFN+WIEKGI++RLSDV EK+FVQLT
Sbjct: 370  ELAFADLNDDMACATAYLQYVVTYILENCKEDMEFFNTWIEKGIINRLSDVAEKNFVQLT 429

Query: 1088 YTDAVELLLRAKKKFEFPVKWGCDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQN 1267
            YTDAVELLLRA KKFEFPVKWGCDLQSEHERYITE AFGGCPVIIRDYPK+IKAFYMR+N
Sbjct: 430  YTDAVELLLRANKKFEFPVKWGCDLQSEHERYITEVAFGGCPVIIRDYPKDIKAFYMRRN 489

Query: 1268 DDGKTVAAMDLLVPRVGELIGGSXXXXXXXXXXXXXXXXXXNKESYWWYLDLRRYGSVPH 1447
            DDGKTVAAMD+LVPRVGELIGGS                  +K+SYWWYLDLRRYGSVPH
Sbjct: 490  DDGKTVAAMDMLVPRVGELIGGSQREERLEYLEERLDDLKLSKDSYWWYLDLRRYGSVPH 549

Query: 1448 AGFGLGFERLVQFATGIENIRDAIPFPRTPGSAEF 1552
            AGFGLGFERLVQFATGI+NIRDAIPFPRTPGSAEF
Sbjct: 550  AGFGLGFERLVQFATGIDNIRDAIPFPRTPGSAEF 584


>ref|XP_002327980.1| predicted protein [Populus trichocarpa] gi|222837389|gb|EEE75768.1|
            predicted protein [Populus trichocarpa]
          Length = 574

 Score =  846 bits (2185), Expect = 0.0
 Identities = 410/512 (80%), Positives = 453/512 (88%)
 Frame = +2

Query: 17   RTFSGLIAAALSSGEAIEKQKAGVLESDKVGKFRKRLRIVDIKGGPDEGLDKLGENIVVK 196
            R F  +I  AL S E+ + +K G +  ++VG+FR++L+I DIKGGPDEGLD++G+ I+V 
Sbjct: 64   RFFCSVIYGALQSSESAKVEKKGEI-GNRVGEFRRKLKIADIKGGPDEGLDRVGKTIIVM 122

Query: 197  GWVRTLRVQSSVTFIEVNDGSCLSNMQCVVSSDAQGYDQVENGLTTTGASICVEGILVTS 376
            GWVRTLRVQSSVTFIEVNDGSCLSN+QCV+  +A+GYDQVE+GL TTGASI ++G +V S
Sbjct: 123  GWVRTLRVQSSVTFIEVNDGSCLSNLQCVMDLEAEGYDQVESGLVTTGASIWMQGTVVKS 182

Query: 377  QGSKQXXXXXXXXXXTVGKSDPSFPIQKKRVSREFLRTKSHLRPRTNTFGAVARVRNALA 556
            QGSKQ           VGKSDPS+PIQKKRVSREFLRTK+HLRPRTNTFGAVARVRNALA
Sbjct: 183  QGSKQKVELKVIKIVAVGKSDPSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRNALA 242

Query: 557  YATHKFFNENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSREAIESPADGLPKTKDGLI 736
            YATHKFF ENGFVWVSSPIITASDCEGAGEQFCVTTLIP S+EA  SP DG+PKTKDGLI
Sbjct: 243  YATHKFFQENGFVWVSSPIITASDCEGAGEQFCVTTLIPGSQEAANSPVDGIPKTKDGLI 302

Query: 737  DWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELA 916
            DWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELA
Sbjct: 303  DWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELA 362

Query: 917  FANLDDDMACATAYLQYVVKYVLENCKEDMDFFNSWIEKGIVSRLSDVVEKDFVQLTYTD 1096
            FA+L+DDMACATAYLQYVV+++LENCKEDM+FFN+WIEKGI+ RLSDVVEKDFVQLTYTD
Sbjct: 363  FADLNDDMACATAYLQYVVRHILENCKEDMEFFNTWIEKGIIDRLSDVVEKDFVQLTYTD 422

Query: 1097 AVELLLRAKKKFEFPVKWGCDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDG 1276
            A+ELLL+AKKKFEFP++WGCDLQSEHERYITEE FGGCPVIIRDYPK+IKAFYMRQNDDG
Sbjct: 423  AIELLLKAKKKFEFPLEWGCDLQSEHERYITEEVFGGCPVIIRDYPKDIKAFYMRQNDDG 482

Query: 1277 KTVAAMDLLVPRVGELIGGSXXXXXXXXXXXXXXXXXXNKESYWWYLDLRRYGSVPHAGF 1456
            KTVAAMD+LVPRVGELIGGS                  NKES+WWYLDLRRYGSVPHAGF
Sbjct: 483  KTVAAMDMLVPRVGELIGGSQREERLEYIEDRLDELKLNKESFWWYLDLRRYGSVPHAGF 542

Query: 1457 GLGFERLVQFATGIENIRDAIPFPRTPGSAEF 1552
            GLGFERLVQFATGI+NIRDAIPFPR PGSAEF
Sbjct: 543  GLGFERLVQFATGIDNIRDAIPFPRVPGSAEF 574


>ref|XP_002309798.1| predicted protein [Populus trichocarpa] gi|222852701|gb|EEE90248.1|
            predicted protein [Populus trichocarpa]
          Length = 578

 Score =  845 bits (2184), Expect = 0.0
 Identities = 416/517 (80%), Positives = 453/517 (87%), Gaps = 5/517 (0%)
 Frame = +2

Query: 17   RTFSGLIAAALSSGEA-----IEKQKAGVLESDKVGKFRKRLRIVDIKGGPDEGLDKLGE 181
            R F  +I+ AL S E+     +EK+K+ V   ++VG+FRK+L+I DIKGGPDEGLD++G+
Sbjct: 64   RFFCSVISGALQSNESAKVGFLEKEKSDV--GNRVGEFRKKLKIADIKGGPDEGLDRVGK 121

Query: 182  NIVVKGWVRTLRVQSSVTFIEVNDGSCLSNMQCVVSSDAQGYDQVENGLTTTGASICVEG 361
             IVV GWVRTLRVQSSVTFIEVNDGSCLSNMQCV+  +A+GYDQVE+GL TTGASI V+G
Sbjct: 122  TIVVMGWVRTLRVQSSVTFIEVNDGSCLSNMQCVMGLEAEGYDQVESGLITTGASILVQG 181

Query: 362  ILVTSQGSKQXXXXXXXXXXTVGKSDPSFPIQKKRVSREFLRTKSHLRPRTNTFGAVARV 541
             +V SQGSKQ           VGKSDPS+PIQKKRVSREFLRTK+HLRPRTNTFGAVARV
Sbjct: 182  TVVESQGSKQKVELKVNKIVLVGKSDPSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARV 241

Query: 542  RNALAYATHKFFNENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSREAIESPADGLPKT 721
            RNALAYATHKFF ENGFVWVSSPIITASDCEGAGEQFCVTTLIP S+E   SP D +PKT
Sbjct: 242  RNALAYATHKFFQENGFVWVSSPIITASDCEGAGEQFCVTTLIPGSQEVANSPVDRIPKT 301

Query: 722  KDGLIDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMI 901
            KDGLIDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMI
Sbjct: 302  KDGLIDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMI 361

Query: 902  EPELAFANLDDDMACATAYLQYVVKYVLENCKEDMDFFNSWIEKGIVSRLSDVVEKDFVQ 1081
            EPELAFA+L DDMACATAYLQYVVK++LENCKEDM+FFN+WIEKGI+ RL+DV EKDFVQ
Sbjct: 362  EPELAFADLTDDMACATAYLQYVVKHILENCKEDMEFFNTWIEKGIIDRLTDVAEKDFVQ 421

Query: 1082 LTYTDAVELLLRAKKKFEFPVKWGCDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMR 1261
            LTYTDAVELLL+AKKKFEFPV+WGCDLQSEHERYITEEAFGGCPVIIRDYPK+IKAFYMR
Sbjct: 422  LTYTDAVELLLKAKKKFEFPVEWGCDLQSEHERYITEEAFGGCPVIIRDYPKDIKAFYMR 481

Query: 1262 QNDDGKTVAAMDLLVPRVGELIGGSXXXXXXXXXXXXXXXXXXNKESYWWYLDLRRYGSV 1441
            QNDDGKTVAAMD+LVPRVGELIGGS                  NKES+WWYLDLRRYGSV
Sbjct: 482  QNDDGKTVAAMDMLVPRVGELIGGSQREERLEYLEDRLDELKLNKESFWWYLDLRRYGSV 541

Query: 1442 PHAGFGLGFERLVQFATGIENIRDAIPFPRTPGSAEF 1552
            PHAGFGLGFERLVQFATG+ENIRDAIPFPR PGSAEF
Sbjct: 542  PHAGFGLGFERLVQFATGLENIRDAIPFPRVPGSAEF 578


>ref|XP_004147209.1| PREDICTED: asparagine--tRNA ligase, chloroplastic/mitochondrial-like
            [Cucumis sativus] gi|449504980|ref|XP_004162346.1|
            PREDICTED: asparagine--tRNA ligase,
            chloroplastic/mitochondrial-like [Cucumis sativus]
          Length = 562

 Score =  818 bits (2113), Expect = 0.0
 Identities = 398/516 (77%), Positives = 442/516 (85%), Gaps = 6/516 (1%)
 Frame = +2

Query: 23   FSGLIAAALSSGEAIEKQKAGVLES------DKVGKFRKRLRIVDIKGGPDEGLDKLGEN 184
            F     +A+S+GEA EK K  V +       +KVG+FRK+LRI +IKGG DEG+D+LG+ 
Sbjct: 47   FRRSFCSAVSAGEAAEKTKPDVSKDWKVTRGEKVGEFRKKLRISEIKGGRDEGVDRLGQT 106

Query: 185  IVVKGWVRTLRVQSSVTFIEVNDGSCLSNMQCVVSSDAQGYDQVENGLTTTGASICVEGI 364
             VV GWVRTLRVQSSVTF+EVNDGSC+SNMQCV+ S  +GYDQVE+GL TTGAS+ VEG+
Sbjct: 107  FVVMGWVRTLRVQSSVTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVWVEGV 166

Query: 365  LVTSQGSKQXXXXXXXXXXTVGKSDPSFPIQKKRVSREFLRTKSHLRPRTNTFGAVARVR 544
            +V SQGSKQ           VGKSDPSFP+QKK+VSREFLRTK+HLRPRTNTFGAVARVR
Sbjct: 167  VVASQGSKQKVELKLQKVIVVGKSDPSFPVQKKKVSREFLRTKAHLRPRTNTFGAVARVR 226

Query: 545  NALAYATHKFFNENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSREAIESPADGLPKTK 724
            NALAYATHKFF ENGFVWVSSPIITASDCEGAGEQFCVTTLIPSS E   S    +PKTK
Sbjct: 227  NALAYATHKFFQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPARSDVGSIPKTK 286

Query: 725  DGLIDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIE 904
            +G IDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIE
Sbjct: 287  EGFIDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIE 346

Query: 905  PELAFANLDDDMACATAYLQYVVKYVLENCKEDMDFFNSWIEKGIVSRLSDVVEKDFVQL 1084
            PELAFA+LDDDM+CATAYLQYVV+++LENCKED+DFFN+WIEKGI+ RL+++VE DFVQL
Sbjct: 347  PELAFADLDDDMSCATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQL 406

Query: 1085 TYTDAVELLLRAKKKFEFPVKWGCDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQ 1264
             YTDA+ELLLRA KKFE+PVKWG DLQSEHERYITE AFGGCPVIIRDYP++IKAFYMRQ
Sbjct: 407  PYTDAIELLLRANKKFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQ 466

Query: 1265 NDDGKTVAAMDLLVPRVGELIGGSXXXXXXXXXXXXXXXXXXNKESYWWYLDLRRYGSVP 1444
            NDDGKTVAAMD+LVP++GELIGGS                  NKESYWWYLDLRRYGSVP
Sbjct: 467  NDDGKTVAAMDMLVPKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYGSVP 526

Query: 1445 HAGFGLGFERLVQFATGIENIRDAIPFPRTPGSAEF 1552
            HAGFGLGFERLVQF TGI+NIRDAIPFPR+PGSAEF
Sbjct: 527  HAGFGLGFERLVQFVTGIDNIRDAIPFPRSPGSAEF 562


>emb|CAA10904.1| asparaginyl-tRNA synthetase [Arabidopsis thaliana]
          Length = 566

 Score =  793 bits (2047), Expect = 0.0
 Identities = 383/512 (74%), Positives = 433/512 (84%)
 Frame = +2

Query: 17   RTFSGLIAAALSSGEAIEKQKAGVLESDKVGKFRKRLRIVDIKGGPDEGLDKLGENIVVK 196
            R F   ++ +L SG+  + +        KVG+FRK+LRI ++KGG DEGL ++G+++ + 
Sbjct: 55   RCFCTAVSESLGSGDGNKVESYEKRFGSKVGEFRKKLRIAEVKGGADEGLSRVGQSLNIM 114

Query: 197  GWVRTLRVQSSVTFIEVNDGSCLSNMQCVVSSDAQGYDQVENGLTTTGASICVEGILVTS 376
            GWVRTLR QSSVTFIE+NDGSCLSN+QCV++SDA+GYDQVE+G   TGAS+ V+G +V S
Sbjct: 115  GWVRTLRSQSSVTFIEINDGSCLSNLQCVMTSDAEGYDQVESGSILTGASVSVQGTIVAS 174

Query: 377  QGSKQXXXXXXXXXXTVGKSDPSFPIQKKRVSREFLRTKSHLRPRTNTFGAVARVRNALA 556
            QG+KQ           VG+ D S+PIQKKRVSREFLRTK+HLRPRTNTFGAVARVRN LA
Sbjct: 175  QGTKQKVELKVEKIIVVGECDSSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRNTLA 234

Query: 557  YATHKFFNENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSREAIESPADGLPKTKDGLI 736
            YATHKFF E+GFVWV+SPIITASDCEGAGEQFCVTTLIPSS E  ++  D +PKTK GLI
Sbjct: 235  YATHKFFQESGFVWVASPIITASDCEGAGEQFCVTTLIPSSHEYTDTSIDAIPKTKGGLI 294

Query: 737  DWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELA 916
            DWSQDFFGKPAFLTVSGQLN ETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELA
Sbjct: 295  DWSQDFFGKPAFLTVSGQLNGETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELA 354

Query: 917  FANLDDDMACATAYLQYVVKYVLENCKEDMDFFNSWIEKGIVSRLSDVVEKDFVQLTYTD 1096
            FA+LDDDMACATAYLQYVVKYVL+NCKEDM+FF++WIEKGI+ RLSDV EK+F+QL YTD
Sbjct: 355  FADLDDDMACATAYLQYVVKYVLDNCKEDMEFFDTWIEKGIIRRLSDVAEKEFLQLGYTD 414

Query: 1097 AVELLLRAKKKFEFPVKWGCDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDG 1276
            A+E+LL+A KKF+FPVKWG DLQSEHERYITEEAFGG PVIIRDYPKEIKAFYMR+NDDG
Sbjct: 415  AIEILLKANKKFDFPVKWGLDLQSEHERYITEEAFGGRPVIIRDYPKEIKAFYMRENDDG 474

Query: 1277 KTVAAMDLLVPRVGELIGGSXXXXXXXXXXXXXXXXXXNKESYWWYLDLRRYGSVPHAGF 1456
            KTVAAMD+LVPR+GELIGGS                  NKESYWWYLDLRRYGSVPHAGF
Sbjct: 475  KTVAAMDMLVPRIGELIGGSQREERLEVLEARLDELKLNKESYWWYLDLRRYGSVPHAGF 534

Query: 1457 GLGFERLVQFATGIENIRDAIPFPRTPGSAEF 1552
            GLGFERLVQF TGI+NIRD IPFPRTP SAEF
Sbjct: 535  GLGFERLVQFVTGIDNIRDVIPFPRTPASAEF 566