BLASTX nr result
ID: Bupleurum21_contig00005436
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00005436
(2356 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_004144449.1| PREDICTED: uncharacterized protein LOC101208... 315 e-103
ref|XP_002303336.1| predicted protein [Populus trichocarpa] gi|2... 299 1e-95
ref|XP_002533812.1| conserved hypothetical protein [Ricinus comm... 297 1e-93
ref|XP_002891771.1| endonuclease [Arabidopsis lyrata subsp. lyra... 298 9e-93
ref|XP_002270971.2| PREDICTED: uncharacterized protein LOC100241... 337 9e-90
>ref|XP_004144449.1| PREDICTED: uncharacterized protein LOC101208479 [Cucumis sativus]
gi|449523814|ref|XP_004168918.1| PREDICTED:
uncharacterized LOC101208479 [Cucumis sativus]
Length = 577
Score = 315 bits (808), Expect(2) = e-103
Identities = 174/313 (55%), Positives = 216/313 (69%), Gaps = 9/313 (2%)
Frame = -2
Query: 2037 MPLLELISSQHTIQHHRISFGSQILYNANDRGLSYLWRSFRIPTKEREFNVGHIKMHRCR 1858
MPLL IS+ H+ H S GS L ND+ LS W+S IP K +V ++ R
Sbjct: 1 MPLLAEISTTHSWFHVNKSLGS--LTFGNDKELSSSWKSLIIP-KRVNLSV-QPEISRRG 56
Query: 1857 VLINAVATLEPKISLESNAHD---------DHRRLHVDVGSENSQHIEHQSSSKDLSEVD 1705
LI AVATLE K L D + +L V + ++ ++ SSS D E+D
Sbjct: 57 FLIRAVATLESKPLLHDGNRDVSMGEPVEFKNSQLGVAPHNPSTSELQLASSSGDSKELD 116
Query: 1704 DREKLRRMRISKANKGNTPWNLGRKHTPETLQKIREKTRLAMQDPKVKMKLVNLGHSQSN 1525
++E+LRR RISKANKGNTPWN GRKHT ETL++I+E+TRLAMQDPKVKMKL+ LGH+QS
Sbjct: 117 EKERLRRERISKANKGNTPWNKGRKHTAETLRRIKERTRLAMQDPKVKMKLIKLGHAQSE 176
Query: 1524 ETRIKIGVGVRMGWNKRRERLMLQETCHFDWQNLIAEASRRGLAGEEELQWDSYEILNEQ 1345
ETR+KIGVGVRMGW +RRE+ +LQETCHF+WQNLIAEASR+G GEEELQWDSY+ILNE+
Sbjct: 177 ETRLKIGVGVRMGWQRRREKQVLQETCHFEWQNLIAEASRQGYKGEEELQWDSYQILNEE 236
Query: 1344 LEQEWLESVEQXXXXXXXXXXXXXXXSAEQKRKISEAIAAKWADPCYRDRVYNGLSKYHG 1165
L++EWLESVEQ SAEQ++KISE+I+AKWADP YRDRV + L+KYHG
Sbjct: 237 LKKEWLESVEQRKKTPRVVGSRRAPKSAEQRKKISESISAKWADPDYRDRVCSALAKYHG 296
Query: 1164 TAIGAANPRRRQK 1126
T G RR++
Sbjct: 297 TPTGVIRRPRRKR 309
Score = 88.6 bits (218), Expect(2) = e-103
Identities = 69/194 (35%), Positives = 92/194 (47%), Gaps = 17/194 (8%)
Frame = -1
Query: 1042 TSTSKKNNVSNGSAGNTTRRAAQQTRNKRSTAPIYKDPLASSKLEMIKSIRANR---ENK 872
TS+ K+ + N S R Q+ + K+S AP +KDPLASSKLEMIKSIRA R E +
Sbjct: 318 TSSKKEKSDVNSSLAGGFRIENQRLKLKKSKAPRFKDPLASSKLEMIKSIRAQRAMAETQ 377
Query: 871 KTEAITRXXXXXXXXXXXXXXXXXXXXKSPLAQASLVETRKLXXXXXXXXXXXEGGFDDS 692
K EAI R +SP+A+ASL+ETRKL S
Sbjct: 378 KMEAIERARLLIAEAEKAAEALEVAATRSPIARASLLETRKLIAEAIQSIESVNIEQTAS 437
Query: 691 PSSQ--------------TVNSVEETDSKSEGLISKDQIEINGSHALPSNFDDISDFAFE 554
P ++ T N+ EE+ + E QI NG+ PSN D+ DF
Sbjct: 438 PQTEEPNAAASYSCYEVVTPNNKEESLGRKEDQNRAVQIIANGTQWFPSNIDE--DFDCS 495
Query: 553 KFNMQELQSHEDEI 512
KF++Q+L E E+
Sbjct: 496 KFSLQDLLGREKEV 509
>ref|XP_002303336.1| predicted protein [Populus trichocarpa] gi|222840768|gb|EEE78315.1|
predicted protein [Populus trichocarpa]
Length = 600
Score = 299 bits (766), Expect(3) = 1e-95
Identities = 161/276 (58%), Positives = 191/276 (69%), Gaps = 2/276 (0%)
Frame = -2
Query: 1953 NDRGLSYLWRSFRIPTKEREFNVGHIKMHRCRVLINAVATLEPKISLESNAHDDHRRLHV 1774
+D+ L L +S + R+ NV R ++ I AVAT+EPK + L
Sbjct: 57 DDKRLLSLGKSIKF---RRKINV----FERSKLQIKAVATVEPKSLVRKGDGKRKTSLEN 109
Query: 1773 DVGSENSQHIEHQ--SSSKDLSEVDDREKLRRMRISKANKGNTPWNLGRKHTPETLQKIR 1600
+ + NS + Q SS +D +DD+E LRR RIS ANKGNTPWN GRKH+PETLQKIR
Sbjct: 110 EQLAANSDTLPAQVESSGEDSMALDDKENLRRKRISNANKGNTPWNKGRKHSPETLQKIR 169
Query: 1599 EKTRLAMQDPKVKMKLVNLGHSQSNETRIKIGVGVRMGWNKRRERLMLQETCHFDWQNLI 1420
E+TRLAMQDPK+KMKL NLGH+QS ETR KIG GVR+GW KRRE+ M+QE C+F+WQNLI
Sbjct: 170 ERTRLAMQDPKIKMKLANLGHAQSKETREKIGHGVRLGWQKRREKQMVQEGCYFEWQNLI 229
Query: 1419 AEASRRGLAGEEELQWDSYEILNEQLEQEWLESVEQXXXXXXXXXXXXXXXSAEQKRKIS 1240
AEASRRG GEEELQWDSY IL +QLE EW+ESV+Q S EQ+RKIS
Sbjct: 230 AEASRRGYTGEEELQWDSYNILRQQLEDEWVESVQQRKTLPRPKGSKRAPKSLEQRRKIS 289
Query: 1239 EAIAAKWADPCYRDRVYNGLSKYHGTAIGAANPRRR 1132
EAIAAKWADP YR+RVY+GLSKYHGT GAA RR
Sbjct: 290 EAIAAKWADPEYRERVYSGLSKYHGTLAGAARKPRR 325
Score = 70.5 bits (171), Expect(3) = 1e-95
Identities = 68/205 (33%), Positives = 94/205 (45%), Gaps = 5/205 (2%)
Frame = -1
Query: 1033 SKKNNVSNGSAGNTTRRAAQQTRNKRSTAPIYKDPLASSKLEMIKSIRANR---ENKKTE 863
S K S+ G R QQ R +RS P YKDPLASSKLEMIK+IRA R E KK E
Sbjct: 338 SSKRRTSDTEKGYA-RSPIQQLR-RRSRTPSYKDPLASSKLEMIKNIRAQRIATETKKNE 395
Query: 862 AITRXXXXXXXXXXXXXXXXXXXXKSPLAQASLVETRKLXXXXXXXXXXXEGGFDDSPSS 683
AI R KSP+A+ASL E RKL + G + SS
Sbjct: 396 AIERARSLIVEAEKAANALEAAAMKSPIARASLTEARKLISEAIQSIESLDQG--NGVSS 453
Query: 682 QTVNSVEE--TDSKSEGLISKDQIEINGSHALPSNFDDISDFAFEKFNMQELQSHEDEIL 509
++++V + L+S+D+ EIN + + D + + E ED
Sbjct: 454 DSISNVNDRYPSLALTELVSEDEKEINAGN---GSMDQVELRQVNGTMIMETSKDEDLNF 510
Query: 508 ANRSHDVDLMNDEDYLKGMLNSSTS 434
+N + DL+N + L + +S+ S
Sbjct: 511 SNLAFH-DLLNGQGELLPLSSSAYS 534
Score = 30.8 bits (68), Expect(3) = 1e-95
Identities = 19/51 (37%), Positives = 27/51 (52%)
Frame = -2
Query: 321 NGSCVTHASLLPNGAKSELSEKESPPTLKNTSKVNSAKSTNTIKKWVRGKL 169
NGS + LPNG++ + E+E+P +KS T KKWV G+L
Sbjct: 555 NGSLTSEKINLPNGSRVQYVEEETP-----------SKSVAT-KKWVHGRL 593
>ref|XP_002533812.1| conserved hypothetical protein [Ricinus communis]
gi|223526249|gb|EEF28565.1| conserved hypothetical
protein [Ricinus communis]
Length = 595
Score = 297 bits (760), Expect(2) = 1e-93
Identities = 157/266 (59%), Positives = 190/266 (71%)
Frame = -2
Query: 1929 WRSFRIPTKEREFNVGHIKMHRCRVLINAVATLEPKISLESNAHDDHRRLHVDVGSENSQ 1750
W+SF P + N R ++ I AVATLEPK +L + +D S N+
Sbjct: 73 WKSFHFP---KTLNT----CERSKLQIKAVATLEPK-ALHKEEEKSKESVLLDDNS-NAP 123
Query: 1749 HIEHQSSSKDLSEVDDREKLRRMRISKANKGNTPWNLGRKHTPETLQKIREKTRLAMQDP 1570
++ SS D E+D+ EKLRRMRISKANKGNTPWN GRKH+PET+Q+IRE+TRLAMQ+P
Sbjct: 124 AVQADSSDADSLELDENEKLRRMRISKANKGNTPWNKGRKHSPETIQRIRERTRLAMQNP 183
Query: 1569 KVKMKLVNLGHSQSNETRIKIGVGVRMGWNKRRERLMLQETCHFDWQNLIAEASRRGLAG 1390
K++MKL NLGH+QS ETR KIGVGVRM W KRRE+ +QETC F+WQNLIAEASRRG AG
Sbjct: 184 KIRMKLANLGHAQSKETRTKIGVGVRMRWKKRREKKNVQETCLFEWQNLIAEASRRGYAG 243
Query: 1389 EEELQWDSYEILNEQLEQEWLESVEQXXXXXXXXXXXXXXXSAEQKRKISEAIAAKWADP 1210
EEE+QWDSY+IL E+LE EW+ES+EQ S EQ+RKI+EAIAAKWADP
Sbjct: 244 EEEMQWDSYKILTEKLEVEWVESIEQRKTMPRPKGSKRAPKSPEQRRKIAEAIAAKWADP 303
Query: 1209 CYRDRVYNGLSKYHGTAIGAANPRRR 1132
YR+RV + LSKYHGT +G PRRR
Sbjct: 304 EYRERVCSALSKYHGTPVG-IKPRRR 328
Score = 75.5 bits (184), Expect(2) = 1e-93
Identities = 61/192 (31%), Positives = 87/192 (45%), Gaps = 13/192 (6%)
Frame = -1
Query: 1030 KKNNVSNGSAGNTTRRAAQQTRNKRSTAPIYKDPLASSKLEMIKSIRANRE---NKKTEA 860
KK++ N S +T ++ R +RS P+YKDPLA SKLEMIK IR R KKTEA
Sbjct: 339 KKSDTENLSKSDTAG-PMRRPRLRRSKTPVYKDPLARSKLEMIKKIREQRAAAGTKKTEA 397
Query: 859 ITRXXXXXXXXXXXXXXXXXXXXKSPLAQASLVETRKLXXXXXXXXXXXEGGF------D 698
I R SP+AQASL+E RKL + + D
Sbjct: 398 IERARLLIAEAQKAAKALEVAATTSPIAQASLIEARKLIAEAILSIESVDAEYMTSSKDD 457
Query: 697 DSPSSQTVNSV----EETDSKSEGLISKDQIEINGSHALPSNFDDISDFAFEKFNMQELQ 530
PS + EE D + + E+NG+ + S+ D D F ++ ++
Sbjct: 458 IDPSLSPIELAGLIDEEADVNNGNSSQAELKEVNGTKIVASSED--KDLNFTNLSLHDIL 515
Query: 529 SHEDEILANRSH 494
+ E E+L+ RS+
Sbjct: 516 NGEYELLSTRSN 527
>ref|XP_002891771.1| endonuclease [Arabidopsis lyrata subsp. lyrata]
gi|297337613|gb|EFH68030.1| endonuclease [Arabidopsis
lyrata subsp. lyrata]
Length = 570
Score = 298 bits (763), Expect(2) = 9e-93
Identities = 155/300 (51%), Positives = 202/300 (67%)
Frame = -2
Query: 2025 ELISSQHTIQHHRISFGSQILYNANDRGLSYLWRSFRIPTKEREFNVGHIKMHRCRVLIN 1846
++ + Q ++Q H + G+Q + +A + L WR +K +F GH + R +VLI
Sbjct: 9 DIATIQPSLQAHLVPLGAQSIIHA--KTLPNPWRQSCF-SKNLKFYTGHTHVRRGKVLIT 65
Query: 1845 AVATLEPKISLESNAHDDHRRLHVDVGSENSQHIEHQSSSKDLSEVDDREKLRRMRISKA 1666
AVATLE K + S +S + S D +VDDREKLRRMRISKA
Sbjct: 66 AVATLETKYPAQKENEQS--------SSLSSASSKSSDGSADDDKVDDREKLRRMRISKA 117
Query: 1665 NKGNTPWNLGRKHTPETLQKIREKTRLAMQDPKVKMKLVNLGHSQSNETRIKIGVGVRMG 1486
N+GNTPWN GRKH+PETLQKIRE+T++AMQDPK+K+KL NLGH+Q+ ETR+KIG GVRM
Sbjct: 118 NRGNTPWNKGRKHSPETLQKIRERTKIAMQDPKIKLKLANLGHAQNKETRMKIGEGVRMR 177
Query: 1485 WNKRRERLMLQETCHFDWQNLIAEASRRGLAGEEELQWDSYEILNEQLEQEWLESVEQXX 1306
W +R+ER +QETCHF+WQNL+AEA++ G EEELQWDSY+IL++Q + EWLESVEQ
Sbjct: 178 WARRKERRKVQETCHFEWQNLLAEAAKEGYRDEEELQWDSYKILDQQNQLEWLESVEQRK 237
Query: 1305 XXXXXXXXXXXXXSAEQKRKISEAIAAKWADPCYRDRVYNGLSKYHGTAIGAANPRRRQK 1126
S EQ+R+I+EAIAAKWADP YR+RV +GL+KYHG +G RRR +
Sbjct: 238 AAKGAKSNRRAPKSPEQRRRIAEAIAAKWADPSYRERVCSGLAKYHGIPVGVERRRRRPR 297
Score = 71.2 bits (173), Expect(2) = 9e-93
Identities = 56/167 (33%), Positives = 78/167 (46%), Gaps = 14/167 (8%)
Frame = -1
Query: 1030 KKNNVSNGSAGNTTRRAAQQ--TRNKRSTAPIYKDPLASSKLEMIKSIRANR---ENKKT 866
KKN + + R +Q + ++ P+YKDPLASSKLEMIKSIRA R E+KK
Sbjct: 304 KKNPTKKSTRDSEFERQSQVQVVKVRKRKTPVYKDPLASSKLEMIKSIRAKRVAEESKKM 363
Query: 865 EAITRXXXXXXXXXXXXXXXXXXXXKSPLAQASLVETRKL---------XXXXXXXXXXX 713
+A+ R KSP+AQASL+E++KL
Sbjct: 364 DAVERARLLISEAEKAAKVLEIAALKSPVAQASLLESKKLIAEATQLIESLEMRQIASDE 423
Query: 712 EGGFDDSPSSQTVNSVEETDSKSEGLISKDQIEINGSHALPSNFDDI 572
+G + S Q NS ET ++ + Q EING+H P N + +
Sbjct: 424 DGTYPSLLSPQHNNSESETKDTND---QEQQGEINGTHTFPINGESL 467
>ref|XP_002270971.2| PREDICTED: uncharacterized protein LOC100241217 [Vitis vinifera]
gi|297742921|emb|CBI35788.3| unnamed protein product
[Vitis vinifera]
Length = 586
Score = 337 bits (864), Expect = 9e-90
Identities = 178/313 (56%), Positives = 229/313 (73%), Gaps = 11/313 (3%)
Frame = -2
Query: 2037 MPLLELISSQHTIQHHRISFGSQILYNANDRG---------LSYLWRSFRIPTKEREFNV 1885
MPLL++ ++Q + Q+H ++ +Q L + G L+ W+ F+IP K+ +FNV
Sbjct: 1 MPLLDIATAQPSFQNHLVTLSAQTLIHGKVSGKFASGPEKRLASAWKPFQIP-KKVDFNV 59
Query: 1884 GHIKMHRCRVLINAVATLEPKISLES-NAHDDHRRLHVDVGSENSQHIEHQSSSKDLSEV 1708
GH++ + LI AVATLE K S++ + + L + V S + QSSS++ SE+
Sbjct: 60 GHLEARWGKFLIKAVATLEAKCSVQGEDGQKGYNNLPLGVDSRPPGN-PIQSSSEEYSEL 118
Query: 1707 DDREKLRRMRISKANKGNTPWNLGRKHTPETLQKIREKTRLAMQDPKVKMKLVNLGHSQS 1528
D+RE+LRRMRISKANKGNTPWN G+KH+ ETLQ+IRE+T+LAMQDPKVKMKLVNLGH+QS
Sbjct: 119 DERERLRRMRISKANKGNTPWNKGKKHSAETLQRIRERTKLAMQDPKVKMKLVNLGHAQS 178
Query: 1527 NETRIKIGVGVRMGWNKRRERLMLQETCHFDWQNLIAEASRRGLAGEEELQWDSYEILNE 1348
ETR+KIGVGVRMGW +RRE+ MLQETC+F+WQ+LIAEASRRG AGEEELQWDSY+IL+E
Sbjct: 179 EETRVKIGVGVRMGWQRRREKRMLQETCYFEWQSLIAEASRRGYAGEEELQWDSYDILDE 238
Query: 1347 QLEQEWLESVEQXXXXXXXXXXXXXXXSAEQKRKISEAIAAKWADPCYRDRVYNGLSKYH 1168
QLE+EWLESVE+ S EQ+RKISEAI+AKW+DP YR+RV + L+KYH
Sbjct: 239 QLEREWLESVEERKRMPRPKGSKRAPKSPEQRRKISEAISAKWSDPAYRERVCSALAKYH 298
Query: 1167 GTAIGA-ANPRRR 1132
G GA PRRR
Sbjct: 299 GIPEGAPRKPRRR 311
Score = 103 bits (258), Expect = 2e-19
Identities = 75/183 (40%), Positives = 98/183 (53%), Gaps = 13/183 (7%)
Frame = -1
Query: 1006 SAGNTTRRAAQQTRNKRSTAPIYKDPLASSKLEMIKSIRANR---ENKKTEAITRXXXXX 836
SAG+ T+ Q+TR K+S +P+YKDPLA+SKLEMIK+IRA R E KKTEAI R
Sbjct: 333 SAGSETKSQNQKTRLKKSNSPMYKDPLANSKLEMIKNIRAQRVAAETKKTEAIERARLLI 392
Query: 835 XXXXXXXXXXXXXXXKSPLAQASLVETRKLXXXXXXXXXXXEGGFDDS---------PSS 683
+SPLA ASL+ET+KL E G S S+
Sbjct: 393 AEAEKAAKALEVAATRSPLAHASLMETKKLIAEAIQSIESIEAGQISSHENSRDPSFSSA 452
Query: 682 QTVNSVE-ETDSKSEGLISKDQIEINGSHALPSNFDDISDFAFEKFNMQELQSHEDEILA 506
VN VE E D+ EGL DQ ++NG+ L S+ +D F F KF Q+L + + E+L+
Sbjct: 453 VPVNHVEKEMDAGIEGLNQADQRKVNGTKTLVSSKNDNEGFDFGKFTWQDLLNGDMELLS 512
Query: 505 NRS 497
S
Sbjct: 513 TSS 515