BLASTX nr result
ID: Bupleurum21_contig00004977
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00004977
(1422 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002283479.1| PREDICTED: E3 ubiquitin-protein ligase HERC2... 620 e-175
ref|XP_002516745.1| Ran GTPase binding protein, putative [Ricinu... 604 e-170
ref|XP_002329325.1| predicted protein [Populus trichocarpa] gi|2... 600 e-169
ref|XP_003555124.1| PREDICTED: probable E3 ubiquitin-protein lig... 598 e-169
ref|XP_004156186.1| PREDICTED: LOW QUALITY PROTEIN: probable E3 ... 595 e-168
>ref|XP_002283479.1| PREDICTED: E3 ubiquitin-protein ligase HERC2 [Vitis vinifera]
gi|296087299|emb|CBI33673.3| unnamed protein product
[Vitis vinifera]
Length = 395
Score = 620 bits (1598), Expect = e-175
Identities = 299/379 (78%), Positives = 319/379 (84%), Gaps = 8/379 (2%)
Frame = +2
Query: 23 GSGEDGQLGIGNNEEREWVCSIKSLNSQNVRSVVAGSRNSLAICEDGKLFTWGWNQRGTL 202
GSGEDGQLGIGNNEE+EWVC++K+L +QNV SVVAGSRNSLAICEDGKLFTWGWNQRGTL
Sbjct: 12 GSGEDGQLGIGNNEEKEWVCAVKALAAQNVCSVVAGSRNSLAICEDGKLFTWGWNQRGTL 71
Query: 203 GHPPDTKTENIPSQVKALSTVKIVQAAIGGWHCLAVDDQGGAYAWGGNEYGQCGEEPERK 382
GHPP+TKTENIPSQVKAL+ KIVQAAIGGWHCLAVDDQG AYAWGGNEYGQCGEEPERK
Sbjct: 72 GHPPETKTENIPSQVKALANAKIVQAAIGGWHCLAVDDQGRAYAWGGNEYGQCGEEPERK 131
Query: 383 DDSSKQLRRDIVIPQRCATKLSVRQVAAGGTHSVVLTHEGHVWTWGQPWPPGDIKQISTP 562
DD+ + LRRDI IPQRCA+KLSVRQVAAGGTHSVVLT EGHVWTWGQPWPPGDIKQISTP
Sbjct: 132 DDTGRPLRRDITIPQRCASKLSVRQVAAGGTHSVVLTREGHVWTWGQPWPPGDIKQISTP 191
Query: 563 VRVQGLERVKVIAVGAFHNLALLEDGSLWAWGNNEYGQLGTGDTQPRSQPIPVDGLSDLT 742
VRVQGLE+V++IAVGAFHNLALLEDG LWAWGNNEYGQLGTGDTQPRSQPI V GLSDLT
Sbjct: 192 VRVQGLEKVRLIAVGAFHNLALLEDGILWAWGNNEYGQLGTGDTQPRSQPIAVQGLSDLT 251
Query: 743 LVDIAAGGWHSTALTD-------XXXXXXXXXXXXXXXXXXXXXXXXXXXXEDVVQVSCG 901
LVDIAAGGWHSTALTD E++VQ+SCG
Sbjct: 252 LVDIAAGGWHSTALTDDGGVYGWGRGEHGRLGFGDDKSSKMVPQKVQLLVEENIVQISCG 311
Query: 902 GTHSVALTRDGRMFSFGRGDHGRLGYGRKVTTGHPSEVPIDLPP-PNSSSTETEGHWSAK 1078
GTHSVALTRDGRMFSFGRGDHGRLGYGRKVTTGHP EVPI+LPP N S + GHW +K
Sbjct: 312 GTHSVALTRDGRMFSFGRGDHGRLGYGRKVTTGHPMEVPINLPPATNLSGSGAGGHWCSK 371
Query: 1079 LVACGGRHTLAIAAWCPNE 1135
LVACGGRHTLAI W +E
Sbjct: 372 LVACGGRHTLAIVEWLAHE 390
>ref|XP_002516745.1| Ran GTPase binding protein, putative [Ricinus communis]
gi|223544118|gb|EEF45643.1| Ran GTPase binding protein,
putative [Ricinus communis]
Length = 393
Score = 604 bits (1557), Expect = e-170
Identities = 294/381 (77%), Positives = 311/381 (81%), Gaps = 10/381 (2%)
Frame = +2
Query: 23 GSGEDGQLGIGNNEEREWVCSIKSLNSQNVRSVVAGSRNSLAICEDGKLFTWGWNQRGTL 202
G+GEDGQLGIG NEE+EWVC + +L VRSVVAGSRNSLAIC+DGKLFTWGWNQRGTL
Sbjct: 11 GAGEDGQLGIGINEEKEWVCVVNALEPYKVRSVVAGSRNSLAICDDGKLFTWGWNQRGTL 70
Query: 203 GHPPDTKTENIPSQVKALSTVKIVQAAIGGWHCLAVDDQGGAYAWGGNEYGQCGEEPERK 382
GHP +TKTENIPSQVKAL+ V IVQAAIGGWHCLAVDDQG AYAWGGNEYGQCGEEPERK
Sbjct: 71 GHPAETKTENIPSQVKALNNVNIVQAAIGGWHCLAVDDQGRAYAWGGNEYGQCGEEPERK 130
Query: 383 DDSSKQLRRDIVIPQRCATKLSVRQVAAGGTHSVVLTHEGHVWTWGQPWPPGDIKQISTP 562
DD+ K LRRDIVIPQRCA KL VRQVAAGGTHSVVLT EGHVWTWGQPWPPGDIKQIS P
Sbjct: 131 DDTGKTLRRDIVIPQRCAPKLVVRQVAAGGTHSVVLTREGHVWTWGQPWPPGDIKQISVP 190
Query: 563 VRVQGLERVKVIAVGAFHNLALLEDGSLWAWGNNEYGQLGTGDTQPRSQPIPVDGLSDLT 742
VRVQGLE+VK+IAVGAFHNLAL EDG+LWAWGNNEYGQLGTGDTQPRSQPIPV GLS LT
Sbjct: 191 VRVQGLEKVKLIAVGAFHNLALQEDGTLWAWGNNEYGQLGTGDTQPRSQPIPVQGLSGLT 250
Query: 743 LVDIAAGGWHSTALTD--------XXXXXXXXXXXXXXXXXXXXXXXXXXXXEDVVQVSC 898
LVDIAAGGWHSTALTD ED+VQ+SC
Sbjct: 251 LVDIAAGGWHSTALTDDGEVYGWGRGEHGRLGFGDNDKSSKMVPQKVNLLAGEDIVQLSC 310
Query: 899 GGTHSVALTRDGRMFSFGRGDHGRLGYGRKVTTGHPSEVPIDLPPPNSSS--TETEGHWS 1072
GGTHSVALT+DGRMFSFGRGDHGRLGYG+KVTTG P EVP D+PPP S S E EGHW
Sbjct: 311 GGTHSVALTQDGRMFSFGRGDHGRLGYGKKVTTGQPVEVPTDIPPPKSLSGTEEAEGHWI 370
Query: 1073 AKLVACGGRHTLAIAAWCPNE 1135
AKLVACGGRHTLAI W +E
Sbjct: 371 AKLVACGGRHTLAIVEWKVDE 391
>ref|XP_002329325.1| predicted protein [Populus trichocarpa] gi|222870779|gb|EEF07910.1|
predicted protein [Populus trichocarpa]
Length = 392
Score = 600 bits (1548), Expect = e-169
Identities = 291/376 (77%), Positives = 310/376 (82%), Gaps = 9/376 (2%)
Frame = +2
Query: 23 GSGEDGQLGIGNNEEREWVCSIKSLNSQNVRSVVAGSRNSLAICEDGKLFTWGWNQRGTL 202
GSGEDGQLGIGNNEE+EWVC +K+L VRSVVAGSRNSLAIC+DGKLFTWGWNQRGTL
Sbjct: 11 GSGEDGQLGIGNNEEKEWVCIVKALEPYKVRSVVAGSRNSLAICDDGKLFTWGWNQRGTL 70
Query: 203 GHPPDTKTENIPSQVKALSTVKIVQAAIGGWHCLAVDDQGGAYAWGGNEYGQCGEEPERK 382
GHPP+TKTENIPSQVKAL+ V IVQAAIGGWHCLAVDDQG AYAWGGNEYGQCGEEPERK
Sbjct: 71 GHPPETKTENIPSQVKALANVNIVQAAIGGWHCLAVDDQGRAYAWGGNEYGQCGEEPERK 130
Query: 383 DDSSKQLRRDIVIPQRCATKLSVRQVAAGGTHSVVLTHEGHVWTWGQPWPPGDIKQISTP 562
DD+ + LRRDIVIPQRCA+KL VRQVAAGGTHSVVLT EGHVW+WGQPWPPGDIKQIS P
Sbjct: 131 DDTGRPLRRDIVIPQRCASKLVVRQVAAGGTHSVVLTREGHVWSWGQPWPPGDIKQISVP 190
Query: 563 VRVQGLERVKVIAVGAFHNLALLEDGSLWAWGNNEYGQLGTGDTQPRSQPIPVDGLSDLT 742
VRVQGLERV++IAVGAFHNLAL EDG+LWAWGNNEYGQLGTGDTQPRSQPI V GLS LT
Sbjct: 191 VRVQGLERVRLIAVGAFHNLALQEDGTLWAWGNNEYGQLGTGDTQPRSQPITVQGLSGLT 250
Query: 743 LVDIAAGGWHSTALTD--------XXXXXXXXXXXXXXXXXXXXXXXXXXXXEDVVQVSC 898
LVDIAAGGWHSTALTD ED+VQVSC
Sbjct: 251 LVDIAAGGWHSTALTDDGEVYGWGRGEHGRLGFGDNDKSSKMVPQRVNLLVGEDIVQVSC 310
Query: 899 GGTHSVALTRDGRMFSFGRGDHGRLGYGRKVTTGHPSEVPIDLPPP-NSSSTETEGHWSA 1075
GGTHSVALTRDGRM++FGRGDHGRLGYGRKVTTG P EVPID+PPP N + + G W A
Sbjct: 311 GGTHSVALTRDGRMYTFGRGDHGRLGYGRKVTTGQPMEVPIDIPPPKNLTDSGDAGRWIA 370
Query: 1076 KLVACGGRHTLAIAAW 1123
KLVA GGRHTLAI W
Sbjct: 371 KLVASGGRHTLAIVEW 386
Score = 77.8 bits (190), Expect = 6e-12
Identities = 49/181 (27%), Positives = 86/181 (47%), Gaps = 6/181 (3%)
Frame = +2
Query: 23 GSGEDGQLGIGNNEEREWVCSIKSLNSQNVRSVVAGSRNSLAICEDGKLFTWGWNQRGTL 202
G+ E GQLG G+ + R +++ L+ + + AG +S A+ +DG+++ WG + G L
Sbjct: 222 GNNEYGQLGTGDTQPRSQPITVQGLSGLTLVDIAAGGWHSTALTDDGEVYGWGRGEHGRL 281
Query: 203 GHPPDTKTEN-IPSQVKALSTVKIVQAAIGGWHCLAVDDQGGAYAWGGNEYGQCGEEPER 379
G + K+ +P +V L IVQ + GG H +A+ G Y +G ++G+ G +
Sbjct: 282 GFGDNDKSSKMVPQRVNLLVGEDIVQVSCGGTHSVALTRDGRMYTFGRGDHGRLGYGRKV 341
Query: 380 KDDSSKQLRRDIVIPQRC-----ATKLSVRQVAAGGTHSVVLTHEGHVWTWGQPWPPGDI 544
++ DI P+ A + + VA+GG H++ + W GD
Sbjct: 342 TTGQPMEVPIDIPPPKNLTDSGDAGRWIAKLVASGGRHTLAIVE----------WHTGDS 391
Query: 545 K 547
K
Sbjct: 392 K 392
>ref|XP_003555124.1| PREDICTED: probable E3 ubiquitin-protein ligase HERC1-like [Glycine
max]
Length = 395
Score = 598 bits (1543), Expect = e-169
Identities = 295/384 (76%), Positives = 310/384 (80%), Gaps = 13/384 (3%)
Frame = +2
Query: 23 GSGEDGQLGIGNNEEREWVCSIKSLNSQNVRSVVAGSRNSLAICEDGKLFTWGWNQRGTL 202
GSGEDGQLGIG+NEE+EWVC +K+L +RSVVAGSRNSLAI +DGKLFTWGWNQR TL
Sbjct: 11 GSGEDGQLGIGSNEEKEWVCLVKALQPHRIRSVVAGSRNSLAIADDGKLFTWGWNQRATL 70
Query: 203 GHPPDTK----TENIPSQVKALSTVKIVQAAIGGWHCLAVDDQGGAYAWGGNEYGQCGEE 370
GHP +TK TEN PSQVKALS+VKIVQAAIGGWHCLAVDDQG AYAWGGNEYGQCGEE
Sbjct: 71 GHPAETKSENKTENTPSQVKALSSVKIVQAAIGGWHCLAVDDQGRAYAWGGNEYGQCGEE 130
Query: 371 PERKDDSSKQLRRDIVIPQRCATKLSVRQVAAGGTHSVVLTHEGHVWTWGQPWPPGDIKQ 550
PERKD + + LRRDI IPQRCA KL VRQVAAGGTHSVVLT EGHVWTWGQPWPPGDIKQ
Sbjct: 131 PERKDGTGRPLRRDIEIPQRCAPKLVVRQVAAGGTHSVVLTREGHVWTWGQPWPPGDIKQ 190
Query: 551 ISTPVRVQGLERVKVIAVGAFHNLALLEDGSLWAWGNNEYGQLGTGDTQPRSQPIPVDGL 730
IS PVRVQGLE V++IAVGAFHNLAL EDG+LWAWGNNEYGQLGTGDTQPRSQPI V GL
Sbjct: 191 ISVPVRVQGLENVRLIAVGAFHNLALQEDGTLWAWGNNEYGQLGTGDTQPRSQPIRVQGL 250
Query: 731 SDLTLVDIAAGGWHSTALTD--------XXXXXXXXXXXXXXXXXXXXXXXXXXXXEDVV 886
SDLTLVDIAAGGWHSTALTD ED+V
Sbjct: 251 SDLTLVDIAAGGWHSTALTDEGEVYGWGRGEHGRLGFGDSDKSSKMVPQKVQLLAGEDIV 310
Query: 887 QVSCGGTHSVALTRDGRMFSFGRGDHGRLGYGRKVTTGHPSEVPIDLPPP-NSSSTETEG 1063
QVSCGGTHSVALTRDG MFSFGRGDHGRLGYGRKVTTG P EVPIDLPPP + S T TEG
Sbjct: 311 QVSCGGTHSVALTRDGHMFSFGRGDHGRLGYGRKVTTGQPMEVPIDLPPPQDPSGTATEG 370
Query: 1064 HWSAKLVACGGRHTLAIAAWCPNE 1135
HW AKLVACGGRHTLAI W NE
Sbjct: 371 HWIAKLVACGGRHTLAIVEWKSNE 394
>ref|XP_004156186.1| PREDICTED: LOW QUALITY PROTEIN: probable E3 ubiquitin-protein ligase
HERC1-like [Cucumis sativus]
Length = 390
Score = 595 bits (1534), Expect = e-168
Identities = 287/379 (75%), Positives = 308/379 (81%), Gaps = 8/379 (2%)
Frame = +2
Query: 23 GSGEDGQLGIGNNEEREWVCSIKSLNSQNVRSVVAGSRNSLAICEDGKLFTWGWNQRGTL 202
GSGEDGQLG+GNNEEREWVC+IK+L SQNV SVVAGSRNSLAI EDGKLFTWGWNQRGTL
Sbjct: 11 GSGEDGQLGLGNNEEREWVCAIKALESQNVCSVVAGSRNSLAITEDGKLFTWGWNQRGTL 70
Query: 203 GHPPDTKTENIPSQVKALSTVKIVQAAIGGWHCLAVDDQGGAYAWGGNEYGQCGEEPERK 382
GHP TK+EN+PSQVKALS VKIVQAAIGGWHCLAVDDQG AYAWGGNEYGQCGEEPE+K
Sbjct: 71 GHPAGTKSENVPSQVKALSNVKIVQAAIGGWHCLAVDDQGRAYAWGGNEYGQCGEEPEKK 130
Query: 383 DDSSKQLRRDIVIPQRCATKLSVRQVAAGGTHSVVLTHEGHVWTWGQPWPPGDIKQISTP 562
D++ + RRDI IPQRCA KL VRQVAAGGTHSVVLT EGHVWTWGQPWPPGDI QIS P
Sbjct: 131 DNTGRPPRRDIAIPQRCAPKLIVRQVAAGGTHSVVLTREGHVWTWGQPWPPGDITQISVP 190
Query: 563 VRVQGLERVKVIAVGAFHNLALLEDGSLWAWGNNEYGQLGTGDTQPRSQPIPVDGLSDLT 742
VRVQGL+ V++IAVGAFHNLAL EDG+LWAWGNNEYGQLGTGDTQPRSQPIPV GLS LT
Sbjct: 191 VRVQGLDMVRLIAVGAFHNLALQEDGTLWAWGNNEYGQLGTGDTQPRSQPIPVQGLSGLT 250
Query: 743 LVDIAAGGWHSTALTD--------XXXXXXXXXXXXXXXXXXXXXXXXXXXXEDVVQVSC 898
LVDIAAGGWHSTALTD E++VQ+SC
Sbjct: 251 LVDIAAGGWHSTALTDNGEVYGWGRGEHGRLGFGDNDKSSKMVPQKVQLLSGEEIVQISC 310
Query: 899 GGTHSVALTRDGRMFSFGRGDHGRLGYGRKVTTGHPSEVPIDLPPPNSSSTETEGHWSAK 1078
GGTHSVA+T DGRMFSFGRGDHGRLGYGRKVTTG P EVPID+PPP + GHW AK
Sbjct: 311 GGTHSVAVTSDGRMFSFGRGDHGRLGYGRKVTTGQPEEVPIDIPPPRGRT--ENGHWIAK 368
Query: 1079 LVACGGRHTLAIAAWCPNE 1135
LVACGGRHTLA+ W P +
Sbjct: 369 LVACGGRHTLALVQWKPED 387