BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00004961
         (1612 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002263747.2| PREDICTED: tRNA dimethylallyltransferase 2 [...   281   e-149
ref|XP_002529084.1| tRNA delta(2)-isopentenylpyrophosphate trans...   270   e-143
gb|ABW77761.1| tRNA isopentenyltransferase [Lotus japonicus]          267   e-142
gb|AAS79605.1| putative tRNA isopentenylpyrophosphatase [Ipomoea...   256   e-132
ref|XP_002880970.1| predicted protein [Arabidopsis lyrata subsp....   250   e-124

>ref|XP_002263747.2| PREDICTED: tRNA dimethylallyltransferase 2 [Vitis vinifera]
           gi|297734839|emb|CBI17073.3| unnamed protein product
           [Vitis vinifera]
          Length = 474

 Score =  281 bits (720), Expect(2) = e-149
 Identities = 137/255 (53%), Positives = 176/255 (69%)
 Frame = -1

Query: 892 QNWGRVDNFRYNCCFLCMDASIPVLDEYVGRRVDCMIAAGLLNEVYDIYNMNVDYSRGLR 713
           +NWGRVDNF+Y CCF+C+DA++P+LD YVG+RVD MI AGLL+EVYDIY++N DY+RGLR
Sbjct: 213 ENWGRVDNFKYRCCFICVDAALPILDGYVGQRVDSMIDAGLLDEVYDIYSLNADYTRGLR 272

Query: 712 QAIGVREFEEFIKAYISKYQNNDDSEFIQNMSIKKPNSIMKEDINAVLCSSIENPLKILL 533
           QAIGVREFE+F+K  +S+  ++            K N ++K ++  +L SS  +   ILL
Sbjct: 273 QAIGVREFEDFLKVLLSEGGHD------------KENGLLKGNVREILKSSNNDQHNILL 320

Query: 532 EDGIEKVKANTXXXXXXXXXXXXXXXXLFGWEMHFVDATESLLCFSDDSWFRKVVDPSAK 353
           ++ IE+VKANT                LFGW++H+VDATES  C    SW   VV+PS K
Sbjct: 321 QEAIERVKANTRRLVRRQKRRFNRLQSLFGWDIHYVDATESSSCNPSASWAADVVEPSVK 380

Query: 352 IIKSFLNEDKSLLPNSEASNGTERLKLVQRDLWTKHICKACGDRVLRGEHEWEQHKQGRS 173
           II+SFL +D S +P+ E  NG E LK + RDLWT++ICKACGDRVLRG HEWEQHKQGR 
Sbjct: 381 IIRSFLRDDASSIPDLEGRNGAEGLKSINRDLWTQYICKACGDRVLRGLHEWEQHKQGRG 440

Query: 172 HRKRVYSLRKSGILC 128
           HRKR+   R     C
Sbjct: 441 HRKRISRSRNLQSSC 455



 Score =  274 bits (701), Expect(2) = e-149
 Identities = 134/195 (68%), Positives = 163/195 (83%)
 Frame = -2

Query: 1479 RPNIVVIMGPTASGKSRLAIDLSTFFPIEIISADSMQVYQGLDVLTNKVPLHEQKGVPHH 1300
            +  +VVIMG T SGKS+LAIDL++ FPIEII++DSMQVYQGLDVLTNKVPLHEQ GVPHH
Sbjct: 19   KTKVVVIMGATGSGKSKLAIDLASHFPIEIINSDSMQVYQGLDVLTNKVPLHEQNGVPHH 78

Query: 1299 LLGTISPDVEFTVKDFRDKAIQLIDEILARNHLPVIVGGTNYYIQSLVSPFLLDESVEDM 1120
            LLGTIS +VEFT KDFRD AI LID+I++RN+LPV+VGGTNYYIQ+LVSPFLLD+S E+M
Sbjct: 79   LLGTISSNVEFTSKDFRDSAIPLIDDIISRNNLPVLVGGTNYYIQALVSPFLLDDSAEEM 138

Query: 1119 DENCSMELPGDKLPDSEIEFQRDSLSYNYEYLQELDPVAANRIHPNDHRKINHYLSVYIR 940
            DE+   + PG    + +++ +R      Y+ L++LDPVAANRIHPNDHRKIN YL +Y R
Sbjct: 139  DESSVHDHPGGNNRNYKLDSKRGGSCSGYDRLKDLDPVAANRIHPNDHRKINQYLGLYAR 198

Query: 939  SGVLPSKLLRGKAME 895
            +GVLPSKL +GKAME
Sbjct: 199  TGVLPSKLFQGKAME 213


>ref|XP_002529084.1| tRNA delta(2)-isopentenylpyrophosphate transferase, putative
           [Ricinus communis] gi|223531496|gb|EEF33328.1| tRNA
           delta(2)-isopentenylpyrophosphate transferase, putative
           [Ricinus communis]
          Length = 453

 Score =  270 bits (690), Expect(2) = e-143
 Identities = 133/254 (52%), Positives = 179/254 (70%), Gaps = 3/254 (1%)
 Frame = -1

Query: 892 QNWGRVDNFRYNCCFLCMDASIPVLDEYVGRRVDCMIAAGLLNEVYDIYNMNVDYSRGLR 713
           +NWGR+DN R++CCF+C+DA+ PVLD+YVG+RVDCMI AGLL EVYDIYN   DY+RGL+
Sbjct: 188 ENWGRIDNCRFDCCFICVDAATPVLDQYVGQRVDCMIDAGLLCEVYDIYNPYADYTRGLQ 247

Query: 712 QAIGVREFEEFIKAYISKYQN---NDDSEFIQNMSIKKPNSIMKEDINAVLCSSIENPLK 542
           QAIGVREFE+ ++AY+S  +N   ND ++    +     + ++K ++  +L SS +N +K
Sbjct: 248 QAIGVREFEDVLRAYLSDNRNDEANDSTDKSLFLVSTNNDKMLKNNMREILRSSDDNEVK 307

Query: 541 ILLEDGIEKVKANTXXXXXXXXXXXXXXXXLFGWEMHFVDATESLLCFSDDSWFRKVVDP 362
           +LL + I+KVK NT                LFGW +H+VDATES+ C SD+SW   VV+P
Sbjct: 308 VLLTEAIDKVKLNTRRLVRRQKRRITRLNTLFGWNIHYVDATESISCKSDESWAAHVVEP 367

Query: 361 SAKIIKSFLNEDKSLLPNSEASNGTERLKLVQRDLWTKHICKACGDRVLRGEHEWEQHKQ 182
           + +II+SFL  DKS +P  EAS        ++R+LWT++ICKACGD+VLRG HEWEQHKQ
Sbjct: 368 AVEIIRSFLRGDKSSVPVLEASVDAG----MKRNLWTQYICKACGDKVLRGAHEWEQHKQ 423

Query: 181 GRSHRKRVYSLRKS 140
           GR HRKR    RK+
Sbjct: 424 GRGHRKRSSRFRKT 437



 Score =  266 bits (680), Expect(2) = e-143
 Identities = 132/189 (69%), Positives = 159/189 (84%), Gaps = 1/189 (0%)
 Frame = -2

Query: 1458 MGPTASGKSRLAIDLSTFFPIEIISADSMQVYQGLDVLTNKVPLHEQKGVPHHLLGTISP 1279
            MGPT SGKS+LAIDL+T FP+EII+ADSMQVY GLDVLTNKVPL++QKGVPHHLLGTISP
Sbjct: 1    MGPTGSGKSKLAIDLATHFPVEIINADSMQVYHGLDVLTNKVPLYDQKGVPHHLLGTISP 60

Query: 1278 DVEFTVKDFRDKAIQLIDEILARNHLPVIVGGTNYYIQSLVSPFLLDE-SVEDMDENCSM 1102
            +VEFT K+FRD AI LI +IL+RN  PVIVGGTNYYIQ+LVSPFLLD+ + ED+D++   
Sbjct: 61   NVEFTAKEFRDSAIPLIKDILSRNCFPVIVGGTNYYIQALVSPFLLDDTAAEDLDDSFVN 120

Query: 1101 ELPGDKLPDSEIEFQRDSLSYNYEYLQELDPVAANRIHPNDHRKINHYLSVYIRSGVLPS 922
            E PGD+  D   EF+ +    +Y+YL+ELDPVAANR+HPN+HRKIN YL++Y RSG+LPS
Sbjct: 121  EPPGDEQTDQVPEFESNGFD-SYDYLKELDPVAANRVHPNNHRKINQYLNLYARSGILPS 179

Query: 921  KLLRGKAME 895
            KL  GKA E
Sbjct: 180  KLYLGKAAE 188


>gb|ABW77761.1| tRNA isopentenyltransferase [Lotus japonicus]
          Length = 477

 Score =  267 bits (683), Expect(2) = e-142
 Identities = 132/259 (50%), Positives = 174/259 (67%)
 Frame = -1

Query: 919 TSTWESHGGQNWGRVDNFRYNCCFLCMDASIPVLDEYVGRRVDCMIAAGLLNEVYDIYNM 740
           +  ++   GQ WG+VDN RY+CCF+C+DAS+PVLD YV +RVDCM+ AGLLNEVYDIYN+
Sbjct: 205 SKVFQGEAGQKWGQVDNLRYDCCFICVDASLPVLDRYVEQRVDCMMDAGLLNEVYDIYNL 264

Query: 739 NVDYSRGLRQAIGVREFEEFIKAYISKYQNNDDSEFIQNMSIKKPNSIMKEDINAVLCSS 560
           N DYSRGLRQAIGVREFE  ++ ++ K     + E     +I+K  ++   ++   L S 
Sbjct: 265 NADYSRGLRQAIGVREFEPLLRTFVLKDIYEREKELTGGSTIEKRETLSNGNLMEWLRSY 324

Query: 559 IENPLKILLEDGIEKVKANTXXXXXXXXXXXXXXXXLFGWEMHFVDATESLLCFSDDSWF 380
            +    IL+E+ IEKVK NT                LFGW +H VD+TES+   SDD W 
Sbjct: 325 SDAKSMILMEEAIEKVKVNTRRLVRRQKRMLNRLQMLFGWNIHSVDSTESISSKSDDVWK 384

Query: 379 RKVVDPSAKIIKSFLNEDKSLLPNSEASNGTERLKLVQRDLWTKHICKACGDRVLRGEHE 200
            +VV+ S KI+ SFL+E+ S+      SN T  +K+VQRDLWT++ CKACGDRVLRG HE
Sbjct: 385 GQVVESSMKIVNSFLSENGSMSSTCSMSNDT-GMKIVQRDLWTQYTCKACGDRVLRGLHE 443

Query: 199 WEQHKQGRSHRKRVYSLRK 143
           WEQH+QGR H KR+ SL++
Sbjct: 444 WEQHRQGRGHGKRISSLKR 462



 Score =  266 bits (681), Expect(2) = e-142
 Identities = 129/198 (65%), Positives = 165/198 (83%), Gaps = 3/198 (1%)
 Frame = -2

Query: 1485 QTRPNIVVIMGPTASGKSRLAIDLSTFFPIEIISADSMQVYQGLDVLTNKVPLHEQKGVP 1306
            + RP +VVI GPT SGKSRLA+DL++ FP+E+I+ADSMQVY GLDVLTNK+P+ EQ GVP
Sbjct: 15   EKRPKVVVITGPTGSGKSRLAVDLASHFPVEVINADSMQVYSGLDVLTNKIPISEQNGVP 74

Query: 1305 HHLLGTISPDVEFTVKDFRDKAIQ-LIDEILARNHLPVIVGGTNYYIQSLVSPFLLDESV 1129
            HHLLGT+SP VEFT K FRD AI  +ID ILARNHLPVIVGGTNYYIQ+LVS FLLDES+
Sbjct: 75   HHLLGTVSPTVEFTAKTFRDSAIPVIIDAILARNHLPVIVGGTNYYIQALVSQFLLDESM 134

Query: 1128 EDMDENCSM-ELPGDKLPDSEIEFQRDSLS-YNYEYLQELDPVAANRIHPNDHRKINHYL 955
            +DM+++C + +LPG    D+    + DS + +NY+ L+++DP+AANRIHPN+HRKIN Y+
Sbjct: 135  DDMNDSCCLGDLPGVMWSDNSFIAESDSSNDFNYDLLKDIDPIAANRIHPNNHRKINQYI 194

Query: 954  SVYIRSGVLPSKLLRGKA 901
            S+Y R+GVLPSK+ +G+A
Sbjct: 195  SLYARTGVLPSKVFQGEA 212


>gb|AAS79605.1| putative tRNA isopentenylpyrophosphatase [Ipomoea trifida]
          Length = 478

 Score =  256 bits (655), Expect(2) = e-132
 Identities = 120/196 (61%), Positives = 158/196 (80%)
 Frame = -2

Query: 1482 TRPNIVVIMGPTASGKSRLAIDLSTFFPIEIISADSMQVYQGLDVLTNKVPLHEQKGVPH 1303
            ++P ++VIMG T +GKS+LAIDL++ FP+++I+ADSMQVYQGLDVLTNKV   EQKGV H
Sbjct: 37   SKPKVIVIMGATGAGKSKLAIDLASHFPVQVINADSMQVYQGLDVLTNKVRPEEQKGVVH 96

Query: 1302 HLLGTISPDVEFTVKDFRDKAIQLIDEILARNHLPVIVGGTNYYIQSLVSPFLLDESVED 1123
            HLLGT+S +VEFT KDFRD AI LIDEI +RN LPVIVGGTNYYIQSLVSPFL+D + ED
Sbjct: 97   HLLGTVSSNVEFTAKDFRDHAIPLIDEISSRNFLPVIVGGTNYYIQSLVSPFLMDVATED 156

Query: 1122 MDENCSMELPGDKLPDSEIEFQRDSLSYNYEYLQELDPVAANRIHPNDHRKINHYLSVYI 943
             + NC ++ P ++ P  E++ +++S  Y Y+ L+ +DPVAANR+HPNDHRK++ YL++Y 
Sbjct: 157  TEVNCLLDPPENEQPCPELKIEQESFDYTYDCLKAIDPVAANRLHPNDHRKVHQYLNLYA 216

Query: 942  RSGVLPSKLLRGKAME 895
            R G+ PSK++  K  E
Sbjct: 217  RFGIPPSKVIEEKTAE 232



 Score =  245 bits (625), Expect(2) = e-132
 Identities = 120/255 (47%), Positives = 168/255 (65%)
 Frame = -1

Query: 907 ESHGGQNWGRVDNFRYNCCFLCMDASIPVLDEYVGRRVDCMIAAGLLNEVYDIYNMNVDY 728
           E    +NWG VDN R++ CF+C+DAS+  LD +V +RVD MI AGLL+EV+DIY  + DY
Sbjct: 227 EEKTAENWGHVDNSRFDFCFICVDASLSALDPFVDKRVDNMIEAGLLDEVFDIYRSDADY 286

Query: 727 SRGLRQAIGVREFEEFIKAYISKYQNNDDSEFIQNMSIKKPNSIMKEDINAVLCSSIENP 548
           ++GLRQAIGVREF +F+  Y  K + + D+   Q  SI+      K+ I  +L  S ++ 
Sbjct: 287 TKGLRQAIGVREFNDFLTFYFHKNEESSDASLSQTWSIE----TFKQTIQDILYGSCDDE 342

Query: 547 LKILLEDGIEKVKANTXXXXXXXXXXXXXXXXLFGWEMHFVDATESLLCFSDDSWFRKVV 368
            K+LL   I+++K NT                LFGWE+H++D T S+L  SD++W  +VV
Sbjct: 343 RKVLLTAAIDQMKLNTRRLVRRQRRRLQRLQMLFGWEIHYLDVTSSILGASDETWAAEVV 402

Query: 367 DPSAKIIKSFLNEDKSLLPNSEASNGTERLKLVQRDLWTKHICKACGDRVLRGEHEWEQH 188
            P+A II+SFLN          A N T+ +KL QRDLW+++IC+ACG++VLRG HEWEQH
Sbjct: 403 KPAATIIESFLNHQSC---PENAGNNTDNIKLKQRDLWSQYICEACGNKVLRGAHEWEQH 459

Query: 187 KQGRSHRKRVYSLRK 143
           +QGR+HRKR+ SL+K
Sbjct: 460 RQGRNHRKRISSLKK 474


>ref|XP_002880970.1| predicted protein [Arabidopsis lyrata subsp. lyrata]
            gi|297326809|gb|EFH57229.1| predicted protein
            [Arabidopsis lyrata subsp. lyrata]
          Length = 470

 Score =  250 bits (639), Expect(2) = e-124
 Identities = 122/199 (61%), Positives = 161/199 (80%), Gaps = 1/199 (0%)
 Frame = -2

Query: 1488 EQTRPNIVVIMGPTASGKSRLAIDLSTFFPIEIISADSMQVYQGLDVLTNKVPLHEQKGV 1309
            ++ +  +VVIMGPT SGKS+LA+DLS+ FP+EII+AD+MQ+Y GLDVLTNK+ ++EQKGV
Sbjct: 18   KKKKAKVVVIMGPTGSGKSKLAVDLSSHFPVEIINADAMQIYSGLDVLTNKITINEQKGV 77

Query: 1308 PHHLLGTISPDVEFTVKDFRDKAIQLIDEILARNHLPVIVGGTNYYIQSLVSPFLLDESV 1129
            PHHLLG++SPD+EFT KDFRD  I LI+EI++RNH+PV+VGGT+YYIQ++VS FLLD+S 
Sbjct: 78   PHHLLGSVSPDMEFTAKDFRDVTIPLIEEIVSRNHIPVLVGGTHYYIQAVVSKFLLDDSA 137

Query: 1128 EDMDENCS-MELPGDKLPDSEIEFQRDSLSYNYEYLQELDPVAANRIHPNDHRKINHYLS 952
            E+ ++ CS +    ++  D E    RD LS+ Y+ L+ELDPVAANRIHPN+ RKIN YLS
Sbjct: 138  ENTEDCCSNVASVVNEDMDVESVSGRDDLSHGYDLLKELDPVAANRIHPNNQRKINLYLS 197

Query: 951  VYIRSGVLPSKLLRGKAME 895
            ++   GVLPSKL +GK  E
Sbjct: 198  LHATRGVLPSKLYQGKTAE 216



 Score =  223 bits (568), Expect(2) = e-124
 Identities = 110/257 (42%), Positives = 164/257 (63%)
 Frame = -1

Query: 910 WESHGGQNWGRVDNFRYNCCFLCMDASIPVLDEYVGRRVDCMIAAGLLNEVYDIYNMNVD 731
           ++    +NWG +D  R++ C +CMDA   VLD YV +RVD M+ AGLL+EVYDIY    D
Sbjct: 210 YQGKTAENWGCIDASRFDYCLICMDAETAVLDRYVEQRVDSMVDAGLLDEVYDIYKPGAD 269

Query: 730 YSRGLRQAIGVREFEEFIKAYISKYQNNDDSEFIQNMSIKKPNSIMKEDINAVLCSSIEN 551
           Y+RGLRQ+IGVREFE+F+K  I +          Q  S    + +MKE++  +L    ++
Sbjct: 270 YTRGLRQSIGVREFEDFLKLNILERGGG------QLTSSSNNDKVMKENLRKILNFPKDD 323

Query: 550 PLKILLEDGIEKVKANTXXXXXXXXXXXXXXXXLFGWEMHFVDATESLLCFSDDSWFRKV 371
            LKI+LE+ I+KVK NT                +F W +H++DATE +L  S++SW  +V
Sbjct: 324 KLKIMLEEAIDKVKLNTRRLLRRQKRRVSRLETVFRWNIHYIDATEYILSKSEESWDAQV 383

Query: 370 VDPSAKIIKSFLNEDKSLLPNSEASNGTERLKLVQRDLWTKHICKACGDRVLRGEHEWEQ 191
           V P+++I+K F      L+  ++  +     K ++RDLW++++C+ACG++VLRG+HEWEQ
Sbjct: 384 VKPASEILKRF------LMTETDTGHDLTSGKSIERDLWSQYVCEACGNKVLRGKHEWEQ 437

Query: 190 HKQGRSHRKRVYSLRKS 140
           HKQGR+HRKR   L+K+
Sbjct: 438 HKQGRTHRKRASRLKKA 454