BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00004954
         (1527 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002275617.2| PREDICTED: ubiquitin-activating enzyme E1 1 ...   660   0.0  
ref|XP_002526617.1| ubiquitin-activating enzyme E1, putative [Ri...   656   0.0  
ref|XP_003615800.1| Ubiquitin-activating enzyme E1 [Medicago tru...   644   0.0  
ref|XP_002313230.1| predicted protein [Populus trichocarpa] gi|2...   642   0.0  
ref|XP_003551222.1| PREDICTED: ubiquitin-activating enzyme E1 1-...   641   0.0  

>ref|XP_002275617.2| PREDICTED: ubiquitin-activating enzyme E1 1 [Vitis vinifera]
          Length = 1100

 Score =  660 bits (1702), Expect = 0.0
 Identities = 341/456 (74%), Positives = 367/456 (80%)
 Frame = +2

Query: 158  LLHYMLPKKRPVEXXXXXXXXXXXXXXXXXLVRKHKIGCLIXXXXXXXXXXXXXXXXXXX 337
            LLHYMLP+KR V                   ++KH+I                       
Sbjct: 10   LLHYMLPRKRAVAGEVVDDDSDNTGTSS---IKKHRISSSAAGTETTVNNNNSGSSLGNN 66

Query: 338  XXXXXXXXDTSVKKPLLPRMAFDDGKQQDIDEDLHSRQLAVYGRETMRRLFASNVLVAGM 517
                     + V+   L  MA  DG   DIDEDLHSRQLAVYGRETMRRLFASNVLV+G+
Sbjct: 67   SGNSNHSGGSEVE---LQIMALGDGHPPDIDEDLHSRQLAVYGRETMRRLFASNVLVSGL 123

Query: 518  QGLGAEIAKNLILAGVKSVTLHDEGIVELWDLSSNFIFTENDVGKNRALASVQKLQELNN 697
            QGLGAEIAKNLILAGVKSVTLHDEG VELWD+SSNFIF+ENDVGKNRALASVQKLQELNN
Sbjct: 124  QGLGAEIAKNLILAGVKSVTLHDEGTVELWDMSSNFIFSENDVGKNRALASVQKLQELNN 183

Query: 698  TVIVATSTKPLTKEQLSDFQAVVFTDINLENAIEFNDYCHTHQPPIAFIKTEVRGLFGNV 877
             V+++T T  LTKE LSDFQAVVFTDI  E AIEFNDYCH+HQPPIAFIK EVRGLFG+V
Sbjct: 184  AVVISTLTTKLTKEDLSDFQAVVFTDIYFEKAIEFNDYCHSHQPPIAFIKAEVRGLFGSV 243

Query: 878  FCDFGPEFTVVDVDGEEPHTGIIASISNDKPALVSFVDDERLEFQDGDFVVFSEVRGMTE 1057
            FCDFGPEFTV DVDGEEPHTGIIASISND PALVS VDDERLEFQDGD VVFSEV GMTE
Sbjct: 244  FCDFGPEFTVFDVDGEEPHTGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMTE 303

Query: 1058 LNDGKPRKITNARPYSFYLEEDTTNFGQYERGGIVTQVKQPKVLNFKPLKEALKDPGEYL 1237
            LNDGKPRKI NARPYSF LEEDTTNFG YE+GGIVTQVKQPKVLNFKPL+EAL DPG++L
Sbjct: 304  LNDGKPRKIKNARPYSFTLEEDTTNFGTYEKGGIVTQVKQPKVLNFKPLREALSDPGDFL 363

Query: 1238 LSDFSKFDRPPLLHLAFQALDKFISDLGRFPVAGSEEDAQKLISIADTLNESFGDGKVAD 1417
            LSDFSKFDRPPLLHLAFQALD+FIS+LGRFPVAGSEEDAQKLI I+  +NE  GDGK+ D
Sbjct: 364  LSDFSKFDRPPLLHLAFQALDRFISELGRFPVAGSEEDAQKLIFISSNINEGLGDGKLED 423

Query: 1418 INPKLLRQFSFGARAVLNPMAAMFGGIVGQEVMKAC 1525
            INPKLLR F+FGARAVLNPMAAMFGGIVGQEV+KAC
Sbjct: 424  INPKLLRHFAFGARAVLNPMAAMFGGIVGQEVVKAC 459


>ref|XP_002526617.1| ubiquitin-activating enzyme E1, putative [Ricinus communis]
            gi|223534057|gb|EEF35776.1| ubiquitin-activating enzyme
            E1, putative [Ricinus communis]
          Length = 1107

 Score =  656 bits (1692), Expect = 0.0
 Identities = 338/464 (72%), Positives = 370/464 (79%), Gaps = 8/464 (1%)
 Frame = +2

Query: 158  LLHYMLPKKRPVEXXXXXXXXXXXXXXXXXLV--------RKHKIGCLIXXXXXXXXXXX 313
            LLH+MLPKKRPVE                  +        +KH+I               
Sbjct: 10   LLHFMLPKKRPVEGQDLEQEEEKEDANKNNTITNIASSSTKKHRIDSCFVESTTPISSNS 69

Query: 314  XXXXXXXXXXXXXXXXDTSVKKPLLPRMAFDDGKQQDIDEDLHSRQLAVYGRETMRRLFA 493
                            ++ +  P        D  Q DIDEDLHSRQLAVYGRETMRRLFA
Sbjct: 70   NGKANINNGGGSSSTTNSVIMAP-------GDVHQNDIDEDLHSRQLAVYGRETMRRLFA 122

Query: 494  SNVLVAGMQGLGAEIAKNLILAGVKSVTLHDEGIVELWDLSSNFIFTENDVGKNRALASV 673
            SNVLVAGMQGLGAEIAKNLILAGVKSVTLHDEG VELWDLSSNF F+ENDVGKNRALAS+
Sbjct: 123  SNVLVAGMQGLGAEIAKNLILAGVKSVTLHDEGAVELWDLSSNFTFSENDVGKNRALASL 182

Query: 674  QKLQELNNTVIVATSTKPLTKEQLSDFQAVVFTDINLENAIEFNDYCHTHQPPIAFIKTE 853
            QKLQELNN V+V+T T  LTKE+LSDFQAVVFTDINLE A EFNDYCH+HQPPI+FIK E
Sbjct: 183  QKLQELNNAVVVSTLTTELTKEKLSDFQAVVFTDINLEKAYEFNDYCHSHQPPISFIKAE 242

Query: 854  VRGLFGNVFCDFGPEFTVVDVDGEEPHTGIIASISNDKPALVSFVDDERLEFQDGDFVVF 1033
            VRGLFG+VFCDFGPEFTV DVDGEEPHTGI+ASISND PALVS VDDERLEFQDGD VVF
Sbjct: 243  VRGLFGSVFCDFGPEFTVFDVDGEEPHTGIVASISNDNPALVSCVDDERLEFQDGDLVVF 302

Query: 1034 SEVRGMTELNDGKPRKITNARPYSFYLEEDTTNFGQYERGGIVTQVKQPKVLNFKPLKEA 1213
            SE+ GMTELNDGKPRKI NARPYSF L+EDTTNFG YE+GGIVTQVK PKVLNFKPL+EA
Sbjct: 303  SEIHGMTELNDGKPRKIKNARPYSFSLDEDTTNFGTYEKGGIVTQVKPPKVLNFKPLREA 362

Query: 1214 LKDPGEYLLSDFSKFDRPPLLHLAFQALDKFISDLGRFPVAGSEEDAQKLISIADTLNES 1393
            LK+PG++LLSDFSKFDRPPLLHLAFQALDKF+S+ GRFPVAGSEEDAQKLIS+A  +N+S
Sbjct: 363  LKNPGDFLLSDFSKFDRPPLLHLAFQALDKFLSESGRFPVAGSEEDAQKLISLAININQS 422

Query: 1394 FGDGKVADINPKLLRQFSFGARAVLNPMAAMFGGIVGQEVMKAC 1525
             GDG+V DINPKLL+QF+FGARAVLNPMAAMFGGIVGQEV+KAC
Sbjct: 423  LGDGRVKDINPKLLQQFAFGARAVLNPMAAMFGGIVGQEVVKAC 466


>ref|XP_003615800.1| Ubiquitin-activating enzyme E1 [Medicago truncatula]
            gi|355517135|gb|AES98758.1| Ubiquitin-activating enzyme
            E1 [Medicago truncatula]
          Length = 1735

 Score =  644 bits (1661), Expect = 0.0
 Identities = 333/476 (69%), Positives = 375/476 (78%), Gaps = 3/476 (0%)
 Frame = +2

Query: 107  YNLRFHTFLTPQSSDRRLLHYMLPKKRPVEXXXXXXXXXXXXXXXXX---LVRKHKIGCL 277
            + +RFH+FL       RL HYMLP+KR  E                     V+K ++G  
Sbjct: 614  FPIRFHSFLP-----HRLFHYMLPRKRVSEGEVVVEEPINNSNSNSNNPGSVKKARMG-- 666

Query: 278  IXXXXXXXXXXXXXXXXXXXXXXXXXXXDTSVKKPLLPRMAFDDGKQQDIDEDLHSRQLA 457
                                        ++ V       MAF +   Q+IDEDLHSRQLA
Sbjct: 667  ----------ESTVNESNKSVSSSGDSSNSGVNLIAASSMAFGNSNPQEIDEDLHSRQLA 716

Query: 458  VYGRETMRRLFASNVLVAGMQGLGAEIAKNLILAGVKSVTLHDEGIVELWDLSSNFIFTE 637
            VYGRETMRRLFAS+VLV+GM+GLGAEIAKNLILAGVKSVTLHDEG VELWDLSSNF+F+E
Sbjct: 717  VYGRETMRRLFASSVLVSGMRGLGAEIAKNLILAGVKSVTLHDEGTVELWDLSSNFVFSE 776

Query: 638  NDVGKNRALASVQKLQELNNTVIVATSTKPLTKEQLSDFQAVVFTDINLENAIEFNDYCH 817
            ND+GKNRA+ASV KLQELNN V+V + T  LTKEQLS+FQAVVFT+++LE A+EFNDYCH
Sbjct: 777  NDLGKNRAVASVSKLQELNNAVLVLSLTTKLTKEQLSNFQAVVFTEVSLEKAVEFNDYCH 836

Query: 818  THQPPIAFIKTEVRGLFGNVFCDFGPEFTVVDVDGEEPHTGIIASISNDKPALVSFVDDE 997
            +HQPPIAFIKTEVRGLFG+VFCDFGPEFTVVDVDGEEPHTGIIASISND PA+VS VDDE
Sbjct: 837  SHQPPIAFIKTEVRGLFGSVFCDFGPEFTVVDVDGEEPHTGIIASISNDNPAVVSCVDDE 896

Query: 998  RLEFQDGDFVVFSEVRGMTELNDGKPRKITNARPYSFYLEEDTTNFGQYERGGIVTQVKQ 1177
            RLEFQDGD VVFSEV GM ELNDGKPRKI NAR YSF LEEDTTN+G YE+GGIVTQ KQ
Sbjct: 897  RLEFQDGDLVVFSEVHGMKELNDGKPRKIKNARAYSFTLEEDTTNYGAYEKGGIVTQAKQ 956

Query: 1178 PKVLNFKPLKEALKDPGEYLLSDFSKFDRPPLLHLAFQALDKFISDLGRFPVAGSEEDAQ 1357
            PKVLNFKPL+EAL DPGE+LLSDFSKFDRPPLLHLAFQALDKFIS++GRFPVAGSEEDA 
Sbjct: 957  PKVLNFKPLREALNDPGEFLLSDFSKFDRPPLLHLAFQALDKFISEIGRFPVAGSEEDAH 1016

Query: 1358 KLISIADTLNESFGDGKVADINPKLLRQFSFGARAVLNPMAAMFGGIVGQEVMKAC 1525
            K ISIA  +N + GDG++ D+NPKLL+QF+FGARAVLNPMAAMFGGIVGQEV+KAC
Sbjct: 1017 KFISIASDINGNLGDGRLEDVNPKLLQQFAFGARAVLNPMAAMFGGIVGQEVVKAC 1072


>ref|XP_002313230.1| predicted protein [Populus trichocarpa] gi|222849638|gb|EEE87185.1|
            predicted protein [Populus trichocarpa]
          Length = 907

 Score =  642 bits (1655), Expect = 0.0
 Identities = 333/457 (72%), Positives = 367/457 (80%)
 Frame = +2

Query: 155  RLLHYMLPKKRPVEXXXXXXXXXXXXXXXXXLVRKHKIGCLIXXXXXXXXXXXXXXXXXX 334
            RLL++MLPKKRPVE                   +K ++ C +                  
Sbjct: 9    RLLYFMLPKKRPVEAEEAPDNSISDSSSF----KKKRVDCDLESTVICNKKSCVTGNCSN 64

Query: 335  XXXXXXXXXDTSVKKPLLPRMAFDDGKQQDIDEDLHSRQLAVYGRETMRRLFASNVLVAG 514
                     +   K+  L  MA  D    +IDEDLHSRQLAVYGRETMRRLF SNVLV+G
Sbjct: 65   TETET----NNHCKEEHLI-MAPGDSNPMEIDEDLHSRQLAVYGRETMRRLFGSNVLVSG 119

Query: 515  MQGLGAEIAKNLILAGVKSVTLHDEGIVELWDLSSNFIFTENDVGKNRALASVQKLQELN 694
            MQGLGAEIAKNLILAGVKSVTLHDE IVELWDLSSNF+F+ENDVGKNRALASVQKLQ+LN
Sbjct: 120  MQGLGAEIAKNLILAGVKSVTLHDEEIVELWDLSSNFVFSENDVGKNRALASVQKLQDLN 179

Query: 695  NTVIVATSTKPLTKEQLSDFQAVVFTDINLENAIEFNDYCHTHQPPIAFIKTEVRGLFGN 874
            N V ++T T  LT EQLS FQAVVFTD+NL+ AIEFNDYCH H+PPI+FIK EVRGLFG+
Sbjct: 180  NAVSISTLTTELTTEQLSKFQAVVFTDLNLDKAIEFNDYCHNHKPPISFIKAEVRGLFGS 239

Query: 875  VFCDFGPEFTVVDVDGEEPHTGIIASISNDKPALVSFVDDERLEFQDGDFVVFSEVRGMT 1054
            VFCDFGPEFTV DVDGE+ HTGIIASISND PALVSFVDDERLEFQDGD VVFSEV+GMT
Sbjct: 240  VFCDFGPEFTVFDVDGEDAHTGIIASISNDNPALVSFVDDERLEFQDGDLVVFSEVKGMT 299

Query: 1055 ELNDGKPRKITNARPYSFYLEEDTTNFGQYERGGIVTQVKQPKVLNFKPLKEALKDPGEY 1234
            ELNDGKPRKI N RPYSF LEEDTTNF  YE+GGIVTQVKQPKVLNFKPL+EA+KDPGE+
Sbjct: 300  ELNDGKPRKIKNTRPYSFTLEEDTTNFATYEKGGIVTQVKQPKVLNFKPLREAIKDPGEF 359

Query: 1235 LLSDFSKFDRPPLLHLAFQALDKFISDLGRFPVAGSEEDAQKLISIADTLNESFGDGKVA 1414
            LLSDFSKFDRPPLLHLAFQALDKF+SD+GRFPVAGSEEDAQKLIS A  +NE+ GD +V 
Sbjct: 360  LLSDFSKFDRPPLLHLAFQALDKFVSDIGRFPVAGSEEDAQKLISQATHINENSGDARVE 419

Query: 1415 DINPKLLRQFSFGARAVLNPMAAMFGGIVGQEVMKAC 1525
            DINPKLLR F+FGARAVLNPMAAMFGG+VGQEV+KAC
Sbjct: 420  DINPKLLRHFAFGARAVLNPMAAMFGGLVGQEVVKAC 456


>ref|XP_003551222.1| PREDICTED: ubiquitin-activating enzyme E1 1-like [Glycine max]
          Length = 1018

 Score =  641 bits (1654), Expect = 0.0
 Identities = 319/377 (84%), Positives = 343/377 (90%)
 Frame = +2

Query: 395  MAFDDGKQQDIDEDLHSRQLAVYGRETMRRLFASNVLVAGMQGLGAEIAKNLILAGVKSV 574
            MA  +  Q DIDEDLHSRQLAVYGRETMRRLF SNVLV+GMQGLG EIAKNLILAGVKSV
Sbjct: 1    MALGESNQPDIDEDLHSRQLAVYGRETMRRLFGSNVLVSGMQGLGVEIAKNLILAGVKSV 60

Query: 575  TLHDEGIVELWDLSSNFIFTENDVGKNRALASVQKLQELNNTVIVATSTKPLTKEQLSDF 754
            TLHDEG VELWDLSSNF+F+ENDVGKNRA ASV KLQELNN VIV + T  LTKE LS+F
Sbjct: 61   TLHDEGTVELWDLSSNFVFSENDVGKNRAAASVSKLQELNNAVIVQSLTTQLTKEHLSNF 120

Query: 755  QAVVFTDINLENAIEFNDYCHTHQPPIAFIKTEVRGLFGNVFCDFGPEFTVVDVDGEEPH 934
            QAVVFTDI+LE A EFNDYCH+HQPPIAFIKTEVRGLFG+VFCDFGPEFTVVDVDGEEPH
Sbjct: 121  QAVVFTDISLEKAFEFNDYCHSHQPPIAFIKTEVRGLFGSVFCDFGPEFTVVDVDGEEPH 180

Query: 935  TGIIASISNDKPALVSFVDDERLEFQDGDFVVFSEVRGMTELNDGKPRKITNARPYSFYL 1114
            TGIIASISND PALVS VDDERLEFQDGD VVFSEV GM ELNDGKPRKI +AR YSF L
Sbjct: 181  TGIIASISNDNPALVSCVDDERLEFQDGDLVVFSEVHGMKELNDGKPRKIKDARAYSFTL 240

Query: 1115 EEDTTNFGQYERGGIVTQVKQPKVLNFKPLKEALKDPGEYLLSDFSKFDRPPLLHLAFQA 1294
            EEDTTN+G YE+GGIVTQVKQPKVLNFKPLKEA+ DPG++LLSDFSKFDRPPLLHLAFQA
Sbjct: 241  EEDTTNYGTYEKGGIVTQVKQPKVLNFKPLKEAITDPGDFLLSDFSKFDRPPLLHLAFQA 300

Query: 1295 LDKFISDLGRFPVAGSEEDAQKLISIADTLNESFGDGKVADINPKLLRQFSFGARAVLNP 1474
            LDKFIS+LGRFPVAGSE+DAQKLIS+A  +N+S  DGK+ DINPKLLR F+FG+RAVLNP
Sbjct: 301  LDKFISELGRFPVAGSEDDAQKLISVASHINDSLRDGKLEDINPKLLRYFAFGSRAVLNP 360

Query: 1475 MAAMFGGIVGQEVMKAC 1525
            MAAMFGGIVGQEV+KAC
Sbjct: 361  MAAMFGGIVGQEVVKAC 377