BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00004919
         (1455 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002281045.1| PREDICTED: E3 ubiquitin-protein ligase RHF1A...   280   5e-73
ref|XP_002314719.1| predicted protein [Populus trichocarpa] gi|2...   266   1e-68
ref|XP_002517194.1| protein binding protein, putative [Ricinus c...   257   5e-66
ref|XP_002312516.1| predicted protein [Populus trichocarpa] gi|2...   251   3e-64
gb|AFK43681.1| unknown [Lotus japonicus]                              251   4e-64

>ref|XP_002281045.1| PREDICTED: E3 ubiquitin-protein ligase RHF1A [Vitis vinifera]
            gi|297737001|emb|CBI26202.3| unnamed protein product
            [Vitis vinifera]
          Length = 391

 Score =  280 bits (717), Expect = 5e-73
 Identities = 170/365 (46%), Positives = 211/365 (57%), Gaps = 22/365 (6%)
 Frame = -1

Query: 1350 SSDDGNTFDDVCSICLEPFNNNQDPPTVTNCKHEYHLQCILEWSQRSKECPICWQILALK 1171
            ++DD +  +D CSICLEPF+ + DP T+TNC+HEYHLQCILEWSQRSKECPICWQ L L+
Sbjct: 20   AADDAS--EDSCSICLEPFSTD-DPATITNCRHEYHLQCILEWSQRSKECPICWQFLVLQ 76

Query: 1170 DPASQELLAAVENERSLRSRIK-------VDHNYEDREVNHVSPYVDASEFDEHMLRDFA 1012
            DP SQELLAAVE ERS RSR         + H   D   +H + Y D S+ DEH+++   
Sbjct: 77   DPTSQELLAAVEGERSPRSRNSSPTESTFLHHFQGDFGYDHDASYSDDSDLDEHIMQHVV 136

Query: 1011 DXXXXXXXXXXXXXXXSSGTGPSQLHLLPP--------------DGFQNLGHEHSQVNSE 874
                            SSG GPS+  + PP              + +QN G   S+ NS 
Sbjct: 137  AAASGAPYVHRRERQRSSGLGPSESLVFPPTDVPDVQTINTPSPEEYQNSGSVSSESNSP 196

Query: 873  V-SIPSAFAIDPMPPNAFPSVENSISNTTGTRDNTVGLSDFFRPQPPDSPPHTNSSESFS 697
              S  SA    P+  +  PSV N +S+T   RD        F    P SP   + S+  S
Sbjct: 197  AHSDQSAINAQPLS-SGVPSVVNMVSSTAANRDGLAKPRVIFDQPLPSSPRRPSPSDLLS 255

Query: 696  FPESIKSKFSAVSARYKDSITKGTRDFKEKLLARNSSVKELSREVHREMSAGIAGVARMI 517
            F +SIKS+ SA SARYK+SI+KGTR FKEKLL+RN+SVKELS+ V REMSAGIA V RMI
Sbjct: 256  FSDSIKSRLSAASARYKESISKGTRGFKEKLLSRNNSVKELSKGVQREMSAGIASVTRMI 315

Query: 516  ERLDLAAKRTGVSVPSSNTDGGTSNLMSKGKDVVHSNHENTGEKVDDTCSYPPQVRPEIS 337
            ERLD   KRTG S P S+  GGTSN+  KGK V  +    +G   + +    P   P  S
Sbjct: 316  ERLDPILKRTGASNPDSSFTGGTSNVSHKGKGVQENVITPSGNNGEISNDSSPDASPLAS 375

Query: 336  LQNQD 322
            +   D
Sbjct: 376  VTISD 380


>ref|XP_002314719.1| predicted protein [Populus trichocarpa] gi|222863759|gb|EEF00890.1|
            predicted protein [Populus trichocarpa]
          Length = 418

 Score =  266 bits (680), Expect = 1e-68
 Identities = 183/419 (43%), Positives = 231/419 (55%), Gaps = 33/419 (7%)
 Frame = -1

Query: 1452 SLTLPFQFSS*ESRV*LL*MADSISLPEKSFPIISSDDGNTFDDVCSICLEPFNNNQDPP 1273
            SLT PF      S   ++  A + S    S  + +  D    DD CSICL+PF   QDP 
Sbjct: 6    SLTSPF------SDTPMVSAAAAASSSSSSSSLGAGVDDAFEDDSCSICLDPFTA-QDPA 58

Query: 1272 TVTNCKHEYHLQCILEWSQRSKECPICWQILALKDPASQELLAAVENERSLRSR------ 1111
            TVT C HEYHLQCILEWSQRSKECPICWQ+L LKD ASQELLAAVE ER LRSR      
Sbjct: 59   TVTCCNHEYHLQCILEWSQRSKECPICWQLLVLKDHASQELLAAVETERLLRSRNSTPAS 118

Query: 1110 IKVDHNYEDREVNHVSPYVDASEFDEHMLRDFADXXXXXXXXXXXXXXXSS-GTGPSQL- 937
            + V H  +D ++   S Y D S+FDEH+++  A                 S G GPSQ+ 
Sbjct: 119  MIVPHLDDDYDIEQDS-YSDDSDFDEHIMQHLAAAASSRAHHVHERERQRSNGLGPSQVI 177

Query: 936  ------HLLP-------PDGFQNLGHEHSQVNSEVSIPSAFAIDPMPPNAFPSVEN--SI 802
                  H+         P+  Q L H  S +NS           P P     +V+N  S+
Sbjct: 178  AFTSPEHVATVQQTCTSPEEGQTLIHGSSVINS-----------PTPDTLSVNVQNLSSV 226

Query: 801  SNTTGTRDNTVGLSDFFRP-----QPP-DSPPHTNSSESFSFPESIKSKFSAVSARYKDS 640
            +     + +T  ++  F+P     QPP D+P    SSE  S  +SIKSK+ A SARYKDS
Sbjct: 227  TPPDVNQVSTTAVNSPFKPRILFRQPPTDTPQEQGSSEVLSLSDSIKSKWFAASARYKDS 286

Query: 639  ITKGTRDFKEKLLARNSSVKELSREVHREMSAGIAGVARMIERLDLAAKRTGVSVPSSNT 460
            ++K TR  KEKL+ARN+SVKELS+EV REMSAGIAGVARMIERLDL  KRTG S+  S  
Sbjct: 287  LSKSTRGIKEKLVARNNSVKELSKEVQREMSAGIAGVARMIERLDLTTKRTGPSMSDSGF 346

Query: 459  DGGTSNLMSKGK----DVVHSNHENTGEKVDDTCSYPPQVRPEISLQNQD*CSRLQIWR 295
             G TSN   KGK    +++      T E++D   S         ++Q +   S +Q+W+
Sbjct: 347  TGATSNFSWKGKGVEQNIIAQALAKTSEEIDRDTSLGASSHASGTVQARVEISHVQVWK 405


>ref|XP_002517194.1| protein binding protein, putative [Ricinus communis]
            gi|223543829|gb|EEF45357.1| protein binding protein,
            putative [Ricinus communis]
          Length = 414

 Score =  257 bits (657), Expect = 5e-66
 Identities = 159/329 (48%), Positives = 196/329 (59%), Gaps = 15/329 (4%)
 Frame = -1

Query: 1335 NTFDDVCSICLEPFNNNQDPPTVTNCKHEYHLQCILEWSQRSKECPICWQILALKDPASQ 1156
            ++F+D CSICLEPF + Q+P TVT+CKHEYHLQCILEWSQRSKECPICWQ+L L++PA Q
Sbjct: 48   DSFEDCCSICLEPFTS-QNPSTVTSCKHEYHLQCILEWSQRSKECPICWQLLVLQEPACQ 106

Query: 1155 ELLAAVENERSLRSR-------IKVDHNYEDREVNHVSPYVDASEFDEHMLRDFADXXXX 997
            ELLAAVE ER LRSR             +ED +V   S Y D S+FDE +++  A     
Sbjct: 107  ELLAAVETERRLRSRNTPSAAFSNPTRFHEDIDVEQDS-YSDDSDFDERIMQHLAAVASR 165

Query: 996  XXXXXXXXXXXSSGTGPSQLHLLP-----PDGFQNLGHEHSQVNSEVSIPSA---FAIDP 841
                       SSG GPS+  + P     P   Q   H  +    +V   S+    A   
Sbjct: 166  ARYICRRERQRSSGQGPSRALIFPSPTNEPSAQQT--HTSAVEGQDVYYGSSRGNSATSL 223

Query: 840  MPPNAFPSVENSISNTTGTRDNTVGLSDFFRPQPPDSPPHTNSSESFSFPESIKSKFSAV 661
            +   + PSV+ ++S    ++           P+PP +     SSE  SF ES+KSK+ A 
Sbjct: 224  LESQSLPSVDTAVSKDVSSK-----------PRPPSTVAPQTSSEGLSFSESMKSKWVAA 272

Query: 660  SARYKDSITKGTRDFKEKLLARNSSVKELSREVHREMSAGIAGVARMIERLDLAAKRTGV 481
            S+RYK+SI+K T+  KEKLLARN+SVKELSR V REMSAGIAGVARM ERLDL  KRTG 
Sbjct: 273  SSRYKESISKSTQGIKEKLLARNNSVKELSRGVQREMSAGIAGVARMFERLDLTPKRTGA 332

Query: 480  SVPSSNTDGGTSNLMSKGKDVVHSNHENT 394
            S P S    GTS L  KGK +     ENT
Sbjct: 333  SSPVSGCREGTSELSLKGKGM----QENT 357


>ref|XP_002312516.1| predicted protein [Populus trichocarpa] gi|222852336|gb|EEE89883.1|
            predicted protein [Populus trichocarpa]
          Length = 301

 Score =  251 bits (642), Expect = 3e-64
 Identities = 164/313 (52%), Positives = 191/313 (61%), Gaps = 10/313 (3%)
 Frame = -1

Query: 1326 DDVCSICLEPFNNNQDPPTVTNCKHEYHLQCILEWSQRSKECPICWQILALKDPASQELL 1147
            DD CSICL+PF   QDP T T CKHEYHLQCI+EWSQRSKECPICWQ+L LKDPASQELL
Sbjct: 1    DDACSICLDPFTP-QDPATDTCCKHEYHLQCIVEWSQRSKECPICWQLLVLKDPASQELL 59

Query: 1146 AAVENERSLRSR------IKVDHNYEDREVNHVSPYVDASEFDEHMLRDF-ADXXXXXXX 988
            AAVE ER  R R      + V H  ED +V   S Y D S+FD+H+++   A        
Sbjct: 60   AAVETERLFRPRNSNPASMIVHHLDEDYDVEQDS-YSDDSDFDDHVMQHLSASAASRARY 118

Query: 987  XXXXXXXXSSGTGPSQLHLLPPDGFQNLGHEH-SQVNSEVSIPSAFAIDPMPPNAFPSVE 811
                    S+G GPSQ+ +           EH S V    + P    I     +   SV 
Sbjct: 119  VCKRERHRSTGLGPSQVLVFTSP-------EHVSTVQQTCTSPEEGQI----LSYGSSVI 167

Query: 810  NSISNTTGTRDNTV-GLSDFFRPQPPDSPPH-TNSSESFSFPESIKSKFSAVSARYKDSI 637
            NS  +T GT    +  LS    P PP  PP    SS+  S  ESIKSK+ A SARYK+S+
Sbjct: 168  NS--STPGTPSVKIQNLSSVAPPVPPIDPPQGQGSSDLSSLSESIKSKWFAASARYKESL 225

Query: 636  TKGTRDFKEKLLARNSSVKELSREVHREMSAGIAGVARMIERLDLAAKRTGVSVPSSNTD 457
            +KGTR  KEKLLARN+SVKELS+EV REMSAGIAGVARMIERLDL +KR G S+  S+  
Sbjct: 226  SKGTRGMKEKLLARNNSVKELSKEVQREMSAGIAGVARMIERLDLTSKRAGPSMSDSDFT 285

Query: 456  GGTSNLMSKGKDV 418
             GTSN   KGK V
Sbjct: 286  RGTSNFSWKGKGV 298


>gb|AFK43681.1| unknown [Lotus japonicus]
          Length = 365

 Score =  251 bits (640), Expect = 4e-64
 Identities = 158/339 (46%), Positives = 199/339 (58%), Gaps = 9/339 (2%)
 Frame = -1

Query: 1341 DGNTFDDVCSICLEPFNNNQDPPTVTNCKHEYHLQCILEWSQRSKECPICWQILALKDPA 1162
            D ++ +D CSICLE  N + DP TVT CKHEYHL CILEWSQRSKECPICWQ LALKDPA
Sbjct: 13   DDDSSEDSCSICLESLNVH-DPSTVTCCKHEYHLHCILEWSQRSKECPICWQSLALKDPA 71

Query: 1161 SQELLAAVENERSLRSRIKVDHNYEDREVN--HVSPYVDASEFDEHMLRDFADXXXXXXX 988
            SQELLAAVE E+ LRSR     ++ +  +N  H   Y +  +FDE +++           
Sbjct: 72   SQELLAAVEAEKRLRSRNVNSSSFANSRLNDGHDGSYSEECDFDEQIMQHLVAAASRARF 131

Query: 987  XXXXXXXXSSGTGPS---QLHLL---PPDGFQNLGHEHSQVNSEVSIPSAFAIDPMPPNA 826
                    S+G GPS    +H+    P     +L    S  +    +PS   ++  PP  
Sbjct: 132  VHRQERRGSAGAGPSANSSVHVRREQPTPTTTSLSQGSSPTSG---VPSN--VNNQPPT- 185

Query: 825  FPSVENSISNTTGTRDNTVGLSDFFRPQPPDSPPHTNSSESFSFPESIKSKFSAVSARYK 646
             P V+    NT+   D        +   PP+S    N+ E FSFPESIKSKFSA SA+YK
Sbjct: 186  -PVVDEVARNTSPQTDVPFRARVIYSQTPPESARRLNTPEMFSFPESIKSKFSAASAKYK 244

Query: 645  DSITKGTRDFKEKLLARNSSVKELSREVHREMSAGIAGVARMIERLDLAAKRTGVSVPSS 466
            +SI+KGTR  KEKLLARN+SVKELS+ V REM+AGIAGVARMIERLDL++KR+    P  
Sbjct: 245  ESISKGTRGLKEKLLARNASVKELSKGVQREMNAGIAGVARMIERLDLSSKRSAALFP-- 302

Query: 465  NTDGGTSNLMSKGKDV-VHSNHENTGEKVDDTCSYPPQV 352
            +  G TS    + K V  HS  E+  + V    S  P +
Sbjct: 303  DRTGETSGFPEEVKSVKEHSPSEDNEDVVHHVTSDAPSL 341