BLASTX nr result
ID: Bupleurum21_contig00004883
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00004883
(2044 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_004150082.1| PREDICTED: importin subunit alpha-1-like [Cu... 153 2e-34
ref|NP_001234006.1| importin subunit alpha [Solanum lycopersicum... 153 2e-34
ref|XP_002275593.1| PREDICTED: importin subunit alpha-1 [Vitis v... 150 1e-33
gb|ABM05488.1| Impa2 [Nicotiana benthamiana] 150 1e-33
ref|XP_002328576.1| predicted protein [Populus trichocarpa] gi|2... 148 5e-33
>ref|XP_004150082.1| PREDICTED: importin subunit alpha-1-like [Cucumis sativus]
gi|449501502|ref|XP_004161385.1| PREDICTED: importin
subunit alpha-1-like [Cucumis sativus]
Length = 530
Score = 153 bits (386), Expect = 2e-34
Identities = 141/496 (28%), Positives = 223/496 (44%), Gaps = 21/496 (4%)
Frame = +3
Query: 96 LRPTMKKVYNHAMFSTPSPVKEAQK----DMNERRKTDRKEECDLIDLICPPDKDIYTSS 263
LRPT + + +E ++ +M E RK+ R+E L + +
Sbjct: 3 LRPTARTEVRRNRYKVSVDAEEGRRRREDNMVEIRKSKREES-----LQKKRREGLQAQQ 57
Query: 264 FVSKALAFNVKRQIESLNSATDLVISSTKD-SVELIDALRNLSSLVNEDPRLGDHLDSQV 440
F S V++++ESL S V S +E R L S + P + + + S V
Sbjct: 58 FPSAVHTSTVEKKLESLPSMVAGVWSDNSSLQLEATTQFRKLLS-IERSPPIEEVIQSGV 116
Query: 441 LPHLVQFLEHDDKAVYEDDITKEGPEDNKSFKLQYYSAVILTKIVSSNKG---IKFKDEA 611
+P V+FL +D +LQ+ +A LT I S + A
Sbjct: 117 VPRFVEFLVREDFP-----------------QLQFEAAWALTNIASGTSEHTKVVIDHGA 159
Query: 612 IPLLLNLIPSPARSVQIQTLHTLTNIADAFPDSSDAMIKNGALEALESILSDKWNHYLVL 791
+P+ + L+ SP+ V+ Q + L N+A P D ++++GAL L S L++ +L
Sbjct: 160 VPIFVKLLASPSDDVREQAVWALGNVAGDSPTCRDLVLRHGALIPLLSQLNEHAK-LSML 218
Query: 792 KGGAQLLTTFCRMKPLLISDKIEIITRTISTAIEYDYPSVLIQLCLALSILCLGGFVAVG 971
+ L+ FCR KP D+++ + + + VL C ALS L G +
Sbjct: 219 RNATWTLSNFCRGKPQPPFDQVKPALPALERLVHSNDEEVLTDACWALSYLSDGTNDKIQ 278
Query: 972 TEVE-----RLIDLICYRESQIVISALQIIGNYVRWGDDELIQFMIEQXXXXXXXXXXYH 1136
+E RL+ L+ + ++I AL+ +GN V GDD Q+++
Sbjct: 279 AVIEAGVCGRLVQLLLHPSPSVLIPALRTVGNIVT-GDDIQTQWIVNDGALSCLLGIFTQ 337
Query: 1137 EHT-IIRKETCWIISNITAVANHSQIQRVINMHFIEILMSLVKNDELEVKEYAACAISNA 1313
H I+KE CW ISNITA N QIQ VI I L++L++N E ++K+ AA AISNA
Sbjct: 338 NHKKSIKKEACWTISNITA-GNKEQIQAVIEARLIPPLVNLLQNAEFDIKKEAAWAISNA 396
Query: 1314 VSGSDKKQFEFLEDKCFIDLEFVRLYCHDDRVFTLCSEVLDKIL-------YRTNNLETN 1472
SG +Q ++L + I L C D R+ T+C E L+ IL N + N
Sbjct: 397 TSGGTHEQIKYLVSQGCIKPLCDLLVCPDPRIVTVCLEGLENILKVGEAEKNLGTNGDVN 456
Query: 1473 VYSIIIKSAFLPKKVE 1520
+Y+ +I A +K+E
Sbjct: 457 LYAQMIDDAEGLEKIE 472
>ref|NP_001234006.1| importin subunit alpha [Solanum lycopersicum]
gi|3915737|sp|O22478.2|IMA_SOLLC RecName: Full=Importin
subunit alpha; AltName: Full=Karyopherin subunit alpha;
Short=KAP alpha gi|3228370|gb|AAC23722.1| importin alpha
[Solanum lycopersicum]
Length = 527
Score = 153 bits (386), Expect = 2e-34
Identities = 144/496 (29%), Positives = 225/496 (45%), Gaps = 21/496 (4%)
Frame = +3
Query: 96 LRPTMKKVYNHAMFSTPSPVKEAQK----DMNERRKTDRKEECDLIDLICPPDKDIYTSS 263
LRP + + + +E ++ +M E RK R+E L + +
Sbjct: 3 LRPNSRTEARRSRYKVAVDAEEGRRRREDNMVEIRKNKREENL----LKKRREGLLQAQQ 58
Query: 264 FVSKALAFNVKRQIESLNSATDLVISSTKD-SVELIDALRNLSSLVNEDPRLGDHLDSQV 440
F S A ++ +++E+L V S +E R L S + +P + + + S V
Sbjct: 59 FPSTAAVSHLDKKLETLPELIAGVWSDDSSLQLECTTQFRKLLS-IERNPPIEEVIQSGV 117
Query: 441 LPHLVQFLEHDDKAVYEDDITKEGPEDNKSFKLQYYSAVILTKIVSS---NKGIKFKDEA 611
+P V+FL DD +LQ+ +A LT I S N + +
Sbjct: 118 VPRFVEFLARDDYP-----------------QLQFEAAWALTNIASGTSENTKVVIDYGS 160
Query: 612 IPLLLNLIPSPARSVQIQTLHTLTNIADAFPDSSDAMIKNGALEALESILSDKWNHYLVL 791
+P+ + L+ SP+ V+ Q + L NIA P D ++ +GAL AL + +++ +L
Sbjct: 161 VPIFIRLLSSPSDDVREQAVWALGNIAGDSPKYRDLVLGHGALVALLAQFNEQAK-LSML 219
Query: 792 KGGAQLLTTFCRMKPLLISDKIEIITRTISTAIEYDYPSVLIQLCLALSILCLGGFVAVG 971
+ L+ FCR KP + ++ + T+ I + VL C ALS L G +
Sbjct: 220 RNATWTLSNFCRGKPQPLFEQTKAALPTLGRLIHSNDEEVLTDACWALSYLSDGTNDKIQ 279
Query: 972 TEVE-----RLIDLICYRESQIVISALQIIGNYVRWGDDELIQFMIEQXXXXXXXXXXYH 1136
+E RL++L+ + ++I AL+ +GN V GDD Q MI+
Sbjct: 280 AVIEAGVCSRLVELLLHSSPSVLIPALRTVGNIVT-GDDIQTQVMIDHHALPCLVNLLTQ 338
Query: 1137 EHT-IIRKETCWIISNITAVANHSQIQRVINMHFIEILMSLVKNDELEVKEYAACAISNA 1313
+ I+KE CW ISNITA N +QIQ VI I L+ L++N E E+K+ AA AISNA
Sbjct: 339 NYKKSIKKEACWTISNITA-GNRNQIQIVIEAGIIAPLVYLLQNAEFEIKKEAAWAISNA 397
Query: 1314 VSGSDKKQFEFLEDKCFIDLEFVRLYCHDDRVFTLCSEVLDKILY-------RTNNLETN 1472
SG + Q +FL + I L C D R+ T+C E L+ IL N N
Sbjct: 398 TSGGNHDQIKFLVSQGCIKPLCDLLVCPDPRIVTVCLEGLENILKIGEADKDLGNTEGVN 457
Query: 1473 VYSIIIKSAFLPKKVE 1520
VY+ +I A +K+E
Sbjct: 458 VYAQLIDEAEGLEKIE 473
>ref|XP_002275593.1| PREDICTED: importin subunit alpha-1 [Vitis vinifera]
gi|296083287|emb|CBI22923.3| unnamed protein product
[Vitis vinifera]
Length = 529
Score = 150 bits (379), Expect = 1e-33
Identities = 142/496 (28%), Positives = 223/496 (44%), Gaps = 21/496 (4%)
Frame = +3
Query: 96 LRPTMKKVYNHAMFSTPSPVKEAQK----DMNERRKTDRKEECDLIDLICPPDKDIYTSS 263
LRP+ + + +E ++ +M E RK+ R+E L + +
Sbjct: 3 LRPSARTEVRRNRYKVAVDAEEGRRRREDNMVEIRKSKREES-----LQKKRREGLQAQQ 57
Query: 264 FVSKALAFNVKRQIESLNSATDLVISSTKD-SVELIDALRNLSSLVNEDPRLGDHLDSQV 440
F + A A V++++ESL S V S +E R L S + P + + + S V
Sbjct: 58 FPAAAHASTVEKKLESLPSMVAGVWSDNSSLQLEATTQFRKLLS-IERSPPIEEVIQSGV 116
Query: 441 LPHLVQFLEHDDKAVYEDDITKEGPEDNKSFKLQYYSAVILTKIVSS---NKGIKFKDEA 611
+P V+FL +D +LQ+ +A LT I S N + A
Sbjct: 117 VPRFVEFLVREDFP-----------------QLQFEAAWALTNIASGTSENTKVVIDHGA 159
Query: 612 IPLLLNLIPSPARSVQIQTLHTLTNIADAFPDSSDAMIKNGALEALESILSDKWNHYLVL 791
+P+ + L+ SP+ V+ Q + L N+A P D ++ +GAL L + L++ +L
Sbjct: 160 VPIFVKLLGSPSDDVREQAVWALGNVAGDSPRCRDLVLGHGALIPLLAQLNEHAK-LSML 218
Query: 792 KGGAQLLTTFCRMKPLLISDKIEIITRTISTAIEYDYPSVLIQLCLALSILCLGGFVAVG 971
+ L+ FCR KP D+++ + + VL C ALS L G +
Sbjct: 219 RNATWTLSNFCRGKPQPPFDQVKPALPALERLVHSSDEEVLTDACWALSYLSDGTNDKIQ 278
Query: 972 TEVE-----RLIDLICYRESQIVISALQIIGNYVRWGDDELIQFMIEQXXXXXXXXXXYH 1136
+E RL++L+ + ++I AL+ +GN V GDD Q +I H
Sbjct: 279 AVIEAGVCPRLVELLLHPSPSVLIPALRTVGNIVT-GDDIQTQSIINHGALPCLLSLLTH 337
Query: 1137 EHT-IIRKETCWIISNITAVANHSQIQRVINMHFIEILMSLVKNDELEVKEYAACAISNA 1313
H I+KE CW ISNITA N QIQ VI I L+ L++ E ++K+ AA AISNA
Sbjct: 338 NHKKSIKKEACWTISNITA-GNKEQIQAVIEAGVIAPLVHLLQTAEFDIKKEAAWAISNA 396
Query: 1314 VSGSDKKQFEFLEDKCFIDLEFVRLYCHDDRVFTLCSEVLDKIL-------YRTNNLETN 1472
SG +Q ++L + I L C D R+ T+C E L+ IL N+ + N
Sbjct: 397 TSGGTHEQIKYLVSQGCIKPLCDLLVCPDPRIVTVCLEGLENILKVGEAEKTLGNSGDVN 456
Query: 1473 VYSIIIKSAFLPKKVE 1520
Y+ +I A +K+E
Sbjct: 457 YYAQLIDDAEGLEKIE 472
>gb|ABM05488.1| Impa2 [Nicotiana benthamiana]
Length = 529
Score = 150 bits (379), Expect = 1e-33
Identities = 129/425 (30%), Positives = 199/425 (46%), Gaps = 15/425 (3%)
Frame = +3
Query: 291 VKRQIESLNSATDLVISSTKD-SVELIDALRNLSSLVNEDPRLGDHLDSQVLPHLVQFLE 467
V++++ESL S V S+ + +E R L S + P + + + S V+P V+FL
Sbjct: 69 VEKKLESLPSMVAGVWSNDNNLQLEATTQFRKLLS-IERSPPIEEVIQSGVVPRFVEFLM 127
Query: 468 HDDKAVYEDDITKEGPEDNKSFKLQYYSAVILTKIVSS---NKGIKFKDEAIPLLLNLIP 638
+D +LQ+ +A LT I S N + A+P+ + L+
Sbjct: 128 REDFP-----------------QLQFEAAWALTNIASGTSDNTRVVIDHGAVPIFVKLLG 170
Query: 639 SPARSVQIQTLHTLTNIADAFPDSSDAMIKNGALEALESILSDKWNHYLVLKGGAQLLTT 818
SP+ V+ Q + L N+A P D ++ NGAL L + L++ +L+ L+
Sbjct: 171 SPSDDVREQAVWALGNVAGDSPRCRDLVLSNGALIPLLAQLNEH-TKLSMLRNATWTLSN 229
Query: 819 FCRMKPLLISDKIEIITRTISTAIEYDYPSVLIQLCLALSILCLGGFVAVGTEVE----- 983
FCR KP +++ + + + VL C ALS L G + +E
Sbjct: 230 FCRGKPQPPFEQVRPALSALQRLVHSNDEEVLTDACWALSYLSDGTNDKIQAVIEAGVCP 289
Query: 984 RLIDLICYRESQIVISALQIIGNYVRWGDDELIQFMIEQXXXXXXXXXXYHEHT-IIRKE 1160
RL++L+ + ++I AL+ +GN V GDD Q +IE H H I+KE
Sbjct: 290 RLVELLMHPSPSVLIPALRTVGNIVT-GDDLQTQCIIEHGALTCLLSLLTHNHKKSIKKE 348
Query: 1161 TCWIISNITAVANHSQIQRVINMHFIEILMSLVKNDELEVKEYAACAISNAVSGSDKKQF 1340
CW ISNITA N QIQ VI I L++L++ E ++K+ AA AISNA SG +Q
Sbjct: 349 ACWTISNITA-GNKEQIQAVIEAGLIAPLVNLLQTAEFDIKKEAAWAISNATSGGTHEQI 407
Query: 1341 EFLEDKCFIDLEFVRLYCHDDRVFTLCSEVLDKIL-----YRTNNLETNVYSIIIKSAFL 1505
+FL + I L C D R+ T+C E L+ IL + N N Y+ + A
Sbjct: 408 KFLVSQGCIKPLCDLLVCPDPRIVTVCLEGLENILKVGEAEKANTGGINYYAQLTDDAEG 467
Query: 1506 PKKVE 1520
+K+E
Sbjct: 468 LEKIE 472
>ref|XP_002328576.1| predicted protein [Populus trichocarpa] gi|222838558|gb|EEE76923.1|
predicted protein [Populus trichocarpa]
Length = 529
Score = 148 bits (374), Expect = 5e-33
Identities = 126/428 (29%), Positives = 200/428 (46%), Gaps = 17/428 (3%)
Frame = +3
Query: 288 NVKRQIESLNSATDLVISSTKD-SVELIDALRNLSSLVNEDPRLGDHLDSQVLPHLVQFL 464
N+++++ESL S V S + +E R L S + P + + + + V+P V+ L
Sbjct: 67 NMEKKLESLPSMVSGVWSDDSNLQLEATTQFRKLLS-IERSPPIEEVIQAGVVPRFVELL 125
Query: 465 EHDDKAVYEDDITKEGPEDNKSFKLQYYSAVILTKIVSS---NKGIKFKDEAIPLLLNLI 635
+D +LQ+ +A LT I S N + A+P+ + L+
Sbjct: 126 VREDFP-----------------QLQFEAAWALTNIASGTSENTKVVIDHGAVPIFVKLL 168
Query: 636 PSPARSVQIQTLHTLTNIADAFPDSSDAMIKNGALEALESILSDKWNHYLVLKGGAQLLT 815
SP+ V+ Q + L N+A P D ++ +GAL L + L++ +L+ L+
Sbjct: 169 GSPSDDVREQAVWALGNVAGDSPKCRDLVLSHGALIPLLAQLNEHAK-LSMLRNATWTLS 227
Query: 816 TFCRMKPLLISDKIEIITRTISTAIEYDYPSVLIQLCLALSILCLGGFVAVGTEVE---- 983
FCR KP + +K+ + + VL C ALS L G + +E
Sbjct: 228 NFCRGKPQPLFEKVRPALPALERLVHSTDEEVLTDACWALSYLSDGTNDKIQAVIEAGVC 287
Query: 984 -RLIDLICYRESQIVISALQIIGNYVRWGDDELIQFMIEQXXXXXXXXXXYHEHT-IIRK 1157
RL++L+ + +++ AL+ +GN V GDD Q +I H H I+K
Sbjct: 288 PRLVELLLHPSPSVLVPALRTVGNIVT-GDDMQTQCIINTGALPCLLSLLTHNHKKSIKK 346
Query: 1158 ETCWIISNITAVANHSQIQRVINMHFIEILMSLVKNDELEVKEYAACAISNAVSGSDKKQ 1337
E CW ISNITA N QIQ VI I L++L++N E ++K+ AA A+SNA SG +Q
Sbjct: 347 EACWTISNITA-GNKEQIQAVIEAGLIGPLVNLLQNAEFDIKKEAAWAVSNATSGGTHEQ 405
Query: 1338 FEFLEDKCFIDLEFVRLYCHDDRVFTLCSEVLDKIL-------YRTNNLETNVYSIIIKS 1496
+FL + I L C D R+ T+C E L+ IL N+ + N Y+ +I
Sbjct: 406 IKFLVSQGCIKPLCDLLVCPDPRIVTVCLEGLENILKVGEAEKNLGNSGDVNFYAQMIDD 465
Query: 1497 AFLPKKVE 1520
A +K+E
Sbjct: 466 AEGLEKIE 473