BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00004819
         (816 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI31610.3| unnamed protein product [Vitis vinifera]              332   5e-89
ref|XP_002277686.2| PREDICTED: folylpolyglutamate synthase, mito...   327   3e-87
ref|XP_002524236.1| folylpolyglutamate synthase, putative [Ricin...   315   6e-84
ref|XP_003544172.1| PREDICTED: folylpolyglutamate synthase, mito...   307   2e-81
ref|XP_002311192.1| folylpolyglutamate synthase [Populus trichoc...   305   8e-81

>emb|CBI31610.3| unnamed protein product [Vitis vinifera]
          Length = 618

 Score =  332 bits (852), Expect = 5e-89
 Identities = 173/270 (64%), Positives = 207/270 (76%), Gaps = 8/270 (2%)
 Frame = -3

Query: 787  GNTLAAIAGEKSGILKRGVPAFTVPQPDEAMHVLEQTASSLDVPLRVATXXXXXXXXXXX 608
            G+TL  IAGEK+GI KRGVPAFTVPQP+EAM VLE  AS LDVPL+V             
Sbjct: 278  GHTLGEIAGEKAGIFKRGVPAFTVPQPEEAMCVLEAKASQLDVPLQVIPPMDASLLNGLK 337

Query: 607  XXXXXXXQYVNAGLAIALSSTWLQKTGHLEFNYLDETSSLPEQFVKGLTTANLQGRAQIV 428
                   QY+NAGLAIAL STWLQ+T HLE NYL++TSSLPEQF+KGLTTA+LQGRAQIV
Sbjct: 338  LGLEGEHQYLNAGLAIALCSTWLQRTSHLEINYLEQTSSLPEQFIKGLTTASLQGRAQIV 397

Query: 427  PDQFFDIERQEDMVFYLDGAHSPESMEVCAKWFALAVKEENE----HCKPL----SNQLL 272
            PD+  +IE   D+VFYLDGAHSPESME+CA+WF+LA+KE+++    + +P     S++ L
Sbjct: 398  PDRCINIESPGDLVFYLDGAHSPESMEICARWFSLAIKEDDQQPTLNYQPQDDVGSSREL 457

Query: 271  DNSAVLESSRKSSSQILLFNCMSVRDPQLLLPRLINTCASHGVHFKQALFVPNVSVYNKV 92
                   +SRK+++QILLFNCMSVRDP LLLP L+NTCASHGVHFKQALFVPN+SVYN+V
Sbjct: 458  VQKDYHGTSRKNTTQILLFNCMSVRDPHLLLPHLMNTCASHGVHFKQALFVPNISVYNRV 517

Query: 91   GTSTLAPIDTQVDLSWQLTLQRLYEFIIRG 2
            G+  L   D QVDLSWQLTLQRL+E +I G
Sbjct: 518  GSHALPLTDLQVDLSWQLTLQRLWESLIHG 547


>ref|XP_002277686.2| PREDICTED: folylpolyglutamate synthase, mitochondrial-like [Vitis
           vinifera]
          Length = 521

 Score =  327 bits (837), Expect = 3e-87
 Identities = 172/262 (65%), Positives = 202/262 (77%)
 Frame = -3

Query: 787 GNTLAAIAGEKSGILKRGVPAFTVPQPDEAMHVLEQTASSLDVPLRVATXXXXXXXXXXX 608
           G+TL  IAGEK+GI KRGVPAFTVPQP+EAM VLE  AS LDVPL+V             
Sbjct: 197 GHTLGEIAGEKAGIFKRGVPAFTVPQPEEAMCVLEAKASQLDVPLQVIPPMDASLLNGLK 256

Query: 607 XXXXXXXQYVNAGLAIALSSTWLQKTGHLEFNYLDETSSLPEQFVKGLTTANLQGRAQIV 428
                  QY+NAGLAIAL STWLQ+T HLE NYL++TSSLPEQF+KGLTTA+LQGRAQIV
Sbjct: 257 LGLEGEHQYLNAGLAIALCSTWLQRTSHLEINYLEQTSSLPEQFIKGLTTASLQGRAQIV 316

Query: 427 PDQFFDIERQEDMVFYLDGAHSPESMEVCAKWFALAVKEENEHCKPLSNQLLDNSAVLES 248
           PD+  +IE   D+VFYLDGAHSPESME+CA+WF+LA+KE+++    L+ Q  D+   + S
Sbjct: 317 PDRCINIESPGDLVFYLDGAHSPESMEICARWFSLAIKEDDQQ-PTLNYQPQDD---VGS 372

Query: 247 SRKSSSQILLFNCMSVRDPQLLLPRLINTCASHGVHFKQALFVPNVSVYNKVGTSTLAPI 68
           SR+    ILLFNCMSVRDP LLLP L+NTCASHGVHFKQALFVPN+SVYN+VG+  L   
Sbjct: 373 SRE---LILLFNCMSVRDPHLLLPHLMNTCASHGVHFKQALFVPNISVYNRVGSHALPLT 429

Query: 67  DTQVDLSWQLTLQRLYEFIIRG 2
           D QVDLSWQLTLQRL+E +I G
Sbjct: 430 DLQVDLSWQLTLQRLWESLIHG 451


>ref|XP_002524236.1| folylpolyglutamate synthase, putative [Ricinus communis]
           gi|223536513|gb|EEF38160.1| folylpolyglutamate synthase,
           putative [Ricinus communis]
          Length = 531

 Score =  315 bits (808), Expect = 6e-84
 Identities = 163/262 (62%), Positives = 194/262 (74%)
 Frame = -3

Query: 787 GNTLAAIAGEKSGILKRGVPAFTVPQPDEAMHVLEQTASSLDVPLRVATXXXXXXXXXXX 608
           GNTL  IAGEK+GI K  +PAFTVPQ DEAMHVLE  AS LDVPL+VA            
Sbjct: 197 GNTLGEIAGEKAGIFKHRIPAFTVPQLDEAMHVLEDKASKLDVPLQVAPPLDPSLLNGLK 256

Query: 607 XXXXXXXQYVNAGLAIALSSTWLQKTGHLEFNYLDETSSLPEQFVKGLTTANLQGRAQIV 428
                  QY+NAGLAIALSS WLQ+TGH+EF+YL+++S LPE F++GLTTA LQGRAQIV
Sbjct: 257 LGLEGEHQYLNAGLAIALSSKWLQRTGHVEFSYLEQSSPLPEHFIRGLTTACLQGRAQIV 316

Query: 427 PDQFFDIERQEDMVFYLDGAHSPESMEVCAKWFALAVKEENEHCKPLSNQLLDNSAVLES 248
           PD+  + E   D+VFYLDGAHSPESME CA+WF+LA+K++N+     S    +  + L  
Sbjct: 317 PDRCVNTETNGDLVFYLDGAHSPESMEACARWFSLAIKDDNQQHTLNSPPHRNYESTL-- 374

Query: 247 SRKSSSQILLFNCMSVRDPQLLLPRLINTCASHGVHFKQALFVPNVSVYNKVGTSTLAPI 68
               S QILLFNCMSVRDPQLLLPRL+ TCAS GV+FK+ALFVPN SVY KVG+  L P+
Sbjct: 375 GFHLSMQILLFNCMSVRDPQLLLPRLMKTCASQGVYFKKALFVPNTSVYYKVGSQALPPV 434

Query: 67  DTQVDLSWQLTLQRLYEFIIRG 2
           D QVDLSWQ TLQRL+E ++RG
Sbjct: 435 DPQVDLSWQFTLQRLWESLVRG 456


>ref|XP_003544172.1| PREDICTED: folylpolyglutamate synthase, mitochondrial-like [Glycine
            max]
          Length = 548

 Score =  307 bits (787), Expect = 2e-81
 Identities = 157/273 (57%), Positives = 201/273 (73%), Gaps = 11/273 (4%)
 Frame = -3

Query: 787  GNTLAAIAGEKSGILKRGVPAFTVPQPDEAMHVLEQTASSLDVPLRVATXXXXXXXXXXX 608
            GNTL  IAGEK+GI K  +PAFTVPQPDEAMHVLE+ AS L+VPL+V T           
Sbjct: 200  GNTLGEIAGEKAGIFKNRIPAFTVPQPDEAMHVLEEKASLLNVPLQVVTPLDAKLLNGLR 259

Query: 607  XXXXXXXQYVNAGLAIALSSTWLQKTGHLEFNYLDETSSLPEQFVKGLTTANLQGRAQIV 428
                   QY+NAGLAIAL  TWL++TGH+   Y+++T +LPEQFVKGLT+A+LQGRAQIV
Sbjct: 260  LGLEGEHQYLNAGLAIALCFTWLKRTGHVGDIYMEQTDTLPEQFVKGLTSASLQGRAQIV 319

Query: 427  PDQFFDIERQEDMVFYLDGAHSPESMEVCAKWFALAVKEENEHCKPLSNQLLDNSAVL-- 254
            PDQ    ER  ++VF+LDGAHSPESMEVCA+WF+LA+KE N   + L +Q L+NS +   
Sbjct: 320  PDQHIKSERSNELVFFLDGAHSPESMEVCARWFSLAIKEYNPD-QTLFHQQLNNSKLSHE 378

Query: 253  ---------ESSRKSSSQILLFNCMSVRDPQLLLPRLINTCASHGVHFKQALFVPNVSVY 101
                       S++ S+ ILLFNC++VRDPQLLLPRL+ TCA HGV+FK+ALFVP++SV+
Sbjct: 379  VVKSTYHGERGSQRKSTPILLFNCLTVRDPQLLLPRLMKTCADHGVYFKKALFVPSLSVF 438

Query: 100  NKVGTSTLAPIDTQVDLSWQLTLQRLYEFIIRG 2
            NKVG     P D++VDLSWQ +LQR++E +++G
Sbjct: 439  NKVGPQAFTPTDSKVDLSWQFSLQRVWENLMQG 471


>ref|XP_002311192.1| folylpolyglutamate synthase [Populus trichocarpa]
            gi|222851012|gb|EEE88559.1| folylpolyglutamate synthase
            [Populus trichocarpa]
          Length = 539

 Score =  305 bits (781), Expect = 8e-81
 Identities = 161/269 (59%), Positives = 197/269 (73%), Gaps = 8/269 (2%)
 Frame = -3

Query: 787  GNTLAAIAGEKSGILKRGVPAFTVPQPDEAMHVLEQTASSLDVPLRVATXXXXXXXXXXX 608
            GNTLA IAGEK+GI K G+PAFTVPQPDEAM+VLE+ AS L+V L+VA            
Sbjct: 195  GNTLAQIAGEKAGIFKDGIPAFTVPQPDEAMNVLEEKASELNVHLQVAEPLDAKLLNGLK 254

Query: 607  XXXXXXXQYVNAGLAIALSSTWLQKTGHLEFNYLDETSSLPEQFVKGLTTANLQGRAQIV 428
                   QY+NAGLAIALS TWLQ+TGH EF ++++ SSLPEQF+KGLTTA+LQGRAQIV
Sbjct: 255  LRLEGEHQYLNAGLAIALSFTWLQRTGHHEFTHMEQASSLPEQFIKGLTTASLQGRAQIV 314

Query: 427  PDQFFDIERQEDMVFYLDGAHSPESMEVCAKWFALAVKEENE----HCKPLSNQLLDNSA 260
             DQ+ + E   D+VFYLDGAHSPESME+CA+WF+LA+KE+++    +  P +N       
Sbjct: 315  TDQYINAESYGDLVFYLDGAHSPESMEMCARWFSLAIKEDSQKRTFNYPPQNNSESTIEL 374

Query: 259  VLESS----RKSSSQILLFNCMSVRDPQLLLPRLINTCASHGVHFKQALFVPNVSVYNKV 92
            V E       KSS QILLFNCMSVRDPQLLLP L+  CASHGV+FK+ALFV N SVY KV
Sbjct: 375  VQEHHDERYGKSSMQILLFNCMSVRDPQLLLPPLMKACASHGVYFKKALFVANTSVYYKV 434

Query: 91   GTSTLAPIDTQVDLSWQLTLQRLYEFIIR 5
            G+  L P D+Q +LSWQ  LQR++E +I+
Sbjct: 435  GSHALPPTDSQFNLSWQFALQRVWENLIQ 463