BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00004746
         (742 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004142420.1| PREDICTED: aspartic proteinase nepenthesin-1...   157   2e-72
ref|XP_004170756.1| PREDICTED: aspartic proteinase nepenthesin-1...   156   5e-72
ref|XP_003538263.1| PREDICTED: aspartic proteinase nepenthesin-1...   148   2e-69
gb|ABK92955.1| unknown [Populus trichocarpa]                          145   2e-69
ref|XP_002299201.1| predicted protein [Populus trichocarpa] gi|1...   145   2e-69

>ref|XP_004142420.1| PREDICTED: aspartic proteinase nepenthesin-1-like [Cucumis sativus]
          Length = 441

 Score =  157 bits (398), Expect(2) = 2e-72
 Identities = 76/103 (73%), Positives = 89/103 (86%)
 Frame = +2

Query: 431 AYIAVRNEFRRRMGKSAVVSSLGGFDTCYSVPITVPTITLMFQGINMTLTQDNFLIHSSA 610
           AY AVR+EFRRR+G++  VSSLGGFDTCY+VPI  PTIT MF G+N+TL  DNFLIHS+A
Sbjct: 332 AYTAVRDEFRRRVGRNVTVSSLGGFDTCYTVPIISPTITFMFAGMNVTLPPDNFLIHSTA 391

Query: 611 TSISCLAMAASPSNVNSVLNVIASMQQQNFRVLFDVPNSRLSV 739
            S +CLAMAA+P NVNSVLNVIASMQQQN R+LFD+PNSR+ V
Sbjct: 392 GSTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIPNSRVGV 434



 Score =  141 bits (355), Expect(2) = 2e-72
 Identities = 69/107 (64%), Positives = 81/107 (75%)
 Frame = +3

Query: 3   TFGCIQKTTGGSVXXXXXXXXXXXXXXXXXXTQSLYKSTFSYCLPTYKSPNFSGDLRLGP 182
           TFGCI+K TG SV                  +QSLY+STFSYCLP++KS NFSG LRLGP
Sbjct: 205 TFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQSLYQSTFSYCLPSFKSVNFSGSLRLGP 264

Query: 183 VGQPIKTKFTPLLKNPSRSSLYYVNLVAIKVGRKIVDIPASALAFNA 323
           V QPI+ K+TPLL+NP RSSLYYVNL++I+VGRKIVDIP SALAFN+
Sbjct: 265 VAQPIRIKYTPLLRNPRRSSLYYVNLISIRVGRKIVDIPPSALAFNS 311


>ref|XP_004170756.1| PREDICTED: aspartic proteinase nepenthesin-1-like, partial [Cucumis
           sativus]
          Length = 364

 Score =  156 bits (395), Expect(2) = 5e-72
 Identities = 75/103 (72%), Positives = 89/103 (86%)
 Frame = +2

Query: 431 AYIAVRNEFRRRMGKSAVVSSLGGFDTCYSVPITVPTITLMFQGINMTLTQDNFLIHSSA 610
           AY AVR+EFRRR+G++  VSSLGGFDTCY+VPI  PTIT MF G+N+TL  DNFLIHS++
Sbjct: 255 AYTAVRDEFRRRVGRNVTVSSLGGFDTCYTVPIISPTITFMFAGMNVTLPPDNFLIHSTS 314

Query: 611 TSISCLAMAASPSNVNSVLNVIASMQQQNFRVLFDVPNSRLSV 739
            S +CLAMAA+P NVNSVLNVIASMQQQN R+LFD+PNSR+ V
Sbjct: 315 GSTTCLAMAAAPDNVNSVLNVIASMQQQNHRILFDIPNSRVGV 357



 Score =  141 bits (355), Expect(2) = 5e-72
 Identities = 69/107 (64%), Positives = 81/107 (75%)
 Frame = +3

Query: 3   TFGCIQKTTGGSVXXXXXXXXXXXXXXXXXXTQSLYKSTFSYCLPTYKSPNFSGDLRLGP 182
           TFGCI+K TG SV                  +QSLY+STFSYCLP++KS NFSG LRLGP
Sbjct: 128 TFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQSLYQSTFSYCLPSFKSVNFSGSLRLGP 187

Query: 183 VGQPIKTKFTPLLKNPSRSSLYYVNLVAIKVGRKIVDIPASALAFNA 323
           V QPI+ K+TPLL+NP RSSLYYVNL++I+VGRKIVDIP SALAFN+
Sbjct: 188 VAQPIRIKYTPLLRNPRRSSLYYVNLISIRVGRKIVDIPPSALAFNS 234


>ref|XP_003538263.1| PREDICTED: aspartic proteinase nepenthesin-1-like [Glycine max]
          Length = 438

 Score =  148 bits (374), Expect(2) = 2e-69
 Identities = 75/106 (70%), Positives = 87/106 (82%), Gaps = 4/106 (3%)
 Frame = +2

Query: 434 YIAVRNEFRRRMGKSA----VVSSLGGFDTCYSVPITVPTITLMFQGINMTLTQDNFLIH 601
           Y AVR+EFRRR+  +A     V+SLGGFDTCY+VPI  PTIT MF G+N+TL QDN LIH
Sbjct: 326 YTAVRDEFRRRVAMAAKANLTVTSLGGFDTCYTVPIVAPTITFMFSGMNVTLPQDNILIH 385

Query: 602 SSATSISCLAMAASPSNVNSVLNVIASMQQQNFRVLFDVPNSRLSV 739
           S+A S SCLAMA++P NVNSVLNVIA+MQQQN RVL+DVPNSRL V
Sbjct: 386 STAGSTSCLAMASAPDNVNSVLNVIANMQQQNHRVLYDVPNSRLGV 431



 Score =  140 bits (353), Expect(2) = 2e-69
 Identities = 70/107 (65%), Positives = 79/107 (73%)
 Frame = +3

Query: 3   TFGCIQKTTGGSVXXXXXXXXXXXXXXXXXXTQSLYKSTFSYCLPTYKSPNFSGDLRLGP 182
           TFGC+ KTTG S                   TQ+LY+STFSYCLP++KS NFSG LRLGP
Sbjct: 198 TFGCVAKTTGPSTPPQGLLGLGRGPLSLLSQTQNLYQSTFSYCLPSFKSLNFSGSLRLGP 257

Query: 183 VGQPIKTKFTPLLKNPSRSSLYYVNLVAIKVGRKIVDIPASALAFNA 323
           V QPI+ K+TPLLKNP RSSLYYVNL AI+VGRKIVDIP +ALAFNA
Sbjct: 258 VAQPIRIKYTPLLKNPRRSSLYYVNLFAIRVGRKIVDIPPAALAFNA 304


>gb|ABK92955.1| unknown [Populus trichocarpa]
          Length = 425

 Score =  145 bits (366), Expect(2) = 2e-69
 Identities = 72/102 (70%), Positives = 86/102 (84%)
 Frame = +2

Query: 434 YIAVRNEFRRRMGKSAVVSSLGGFDTCYSVPITVPTITLMFQGINMTLTQDNFLIHSSAT 613
           Y AVR+EFR+R+G +A+VSSLGGFDTCY+ PI  PT+T MF G+N+TL  DN LI S+A 
Sbjct: 319 YTAVRDEFRKRVG-NAIVSSLGGFDTCYTGPIVAPTMTFMFSGMNVTLPTDNLLIRSTAG 377

Query: 614 SISCLAMAASPSNVNSVLNVIASMQQQNFRVLFDVPNSRLSV 739
           S SCLAMAA+P NVNSVLNVIA+MQQQN R+LFDVPNSR+ V
Sbjct: 378 STSCLAMAAAPDNVNSVLNVIANMQQQNHRILFDVPNSRIGV 419



 Score =  143 bits (361), Expect(2) = 2e-69
 Identities = 71/106 (66%), Positives = 79/106 (74%)
 Frame = +3

Query: 3   TFGCIQKTTGGSVXXXXXXXXXXXXXXXXXXTQSLYKSTFSYCLPTYKSPNFSGDLRLGP 182
           TFGCIQKTTG SV                  TQ LYKSTFSYCLP++++ NFSG LRLGP
Sbjct: 191 TFGCIQKTTGSSVPPQGLLGLGRGPLSFLSQTQDLYKSTFSYCLPSFRTLNFSGTLRLGP 250

Query: 183 VGQPIKTKFTPLLKNPSRSSLYYVNLVAIKVGRKIVDIPASALAFN 320
            GQP++ K TPLLKNP RSSLYYVNL+ I+VGRKIVDIPASALAFN
Sbjct: 251 AGQPLRIKTTPLLKNPRRSSLYYVNLIGIRVGRKIVDIPASALAFN 296


>ref|XP_002299201.1| predicted protein [Populus trichocarpa] gi|118483775|gb|ABK93780.1|
           unknown [Populus trichocarpa]
           gi|222846459|gb|EEE84006.1| predicted protein [Populus
           trichocarpa]
          Length = 425

 Score =  145 bits (366), Expect(2) = 2e-69
 Identities = 72/102 (70%), Positives = 86/102 (84%)
 Frame = +2

Query: 434 YIAVRNEFRRRMGKSAVVSSLGGFDTCYSVPITVPTITLMFQGINMTLTQDNFLIHSSAT 613
           Y AVR+EFR+R+G +A+VSSLGGFDTCY+ PI  PT+T MF G+N+TL  DN LI S+A 
Sbjct: 319 YTAVRDEFRKRVG-NAIVSSLGGFDTCYTGPIVAPTMTFMFSGMNVTLPPDNLLIRSTAG 377

Query: 614 SISCLAMAASPSNVNSVLNVIASMQQQNFRVLFDVPNSRLSV 739
           S SCLAMAA+P NVNSVLNVIA+MQQQN R+LFDVPNSR+ V
Sbjct: 378 STSCLAMAAAPDNVNSVLNVIANMQQQNHRILFDVPNSRIGV 419



 Score =  143 bits (361), Expect(2) = 2e-69
 Identities = 71/106 (66%), Positives = 79/106 (74%)
 Frame = +3

Query: 3   TFGCIQKTTGGSVXXXXXXXXXXXXXXXXXXTQSLYKSTFSYCLPTYKSPNFSGDLRLGP 182
           TFGCIQKTTG SV                  TQ LYKSTFSYCLP++++ NFSG LRLGP
Sbjct: 191 TFGCIQKTTGSSVPPQGLLGLGRGPLSFLSQTQDLYKSTFSYCLPSFRTLNFSGTLRLGP 250

Query: 183 VGQPIKTKFTPLLKNPSRSSLYYVNLVAIKVGRKIVDIPASALAFN 320
            GQP++ K TPLLKNP RSSLYYVNL+ I+VGRKIVDIPASALAFN
Sbjct: 251 AGQPLRIKTTPLLKNPRRSSLYYVNLIGIRVGRKIVDIPASALAFN 296