BLASTX nr result
ID: Bupleurum21_contig00004724
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00004724
(1650 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002277269.1| PREDICTED: putative E3 ubiquitin-protein lig... 432 e-124
ref|XP_002516824.1| conserved hypothetical protein [Ricinus comm... 436 e-124
ref|XP_004159841.1| PREDICTED: LOW QUALITY PROTEIN: putative E3 ... 376 e-108
ref|XP_004134170.1| PREDICTED: putative E3 ubiquitin-protein lig... 376 e-108
ref|XP_003546114.1| PREDICTED: putative E3 ubiquitin-protein lig... 396 e-107
>ref|XP_002277269.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298-like [Vitis
vinifera]
Length = 893
Score = 432 bits (1111), Expect(2) = e-124
Identities = 241/443 (54%), Positives = 296/443 (66%), Gaps = 7/443 (1%)
Frame = -2
Query: 1607 MASLVAKACNSGSGQMMPVIAMQEKGSRNKRKFRADPPSPDTSKTLSLPLGDYSSYEFSA 1428
M+S+ ++ Q P ++ QEKGSRNKRKFRADPP D +K +S SYEFSA
Sbjct: 1 MSSMAVNGSSTCGTQGSPSVSAQEKGSRNKRKFRADPPLGDPNKIVSSQ-DQCLSYEFSA 59
Query: 1427 EKLKSIQNHGHASGCDMCNVGHASGCDMCNVGREHSDSLKLDLGLSCAVGPYEAGTSKPK 1248
EK + +HG C MCN+ ++HSD LKLDLGLS A G E G S+P+
Sbjct: 60 EKFEVTSSHGQPGACGMCNLN-----------QDHSDGLKLDLGLSSAAGSSEVGPSQPR 108
Query: 1247 DEIVYAGALHDADWSDLTESQLEELVLDNLDAILKSAIKKLMDCGYSEELCTRAVVKSGL 1068
DE+ A DADWSDLTESQLEELVL NLD I KSAIKK++ CGYSEE+ T+AV++SGL
Sbjct: 109 DELE-ADDFQDADWSDLTESQLEELVLSNLDTIFKSAIKKIVACGYSEEVATKAVLRSGL 167
Query: 1067 CYGCKDIVSNIVDNALALLRNGQDLDISREHDFEDLLQMEKYILAELVCVIREVKPFFST 888
CYGCKD VSNIVDN LA LRNGQ++D SREH F+DL Q+EKYILAELVCV+REV+PFFST
Sbjct: 168 CYGCKDTVSNIVDNTLAFLRNGQEIDPSREHYFDDLQQLEKYILAELVCVLREVRPFFST 227
Query: 887 GGAMWCLLVCDMNVSLACAMESDPFSNLI-GDRAPYGSPKAQVQPQMKIETRSSENHLNV 711
G AMWCLL+CDMNVS ACAM+ D FS+++ GD A GS QPQ K E +SSE +L
Sbjct: 228 GDAMWCLLICDMNVSHACAMDGDSFSSIVSGDGASNGSSSTSGQPQSKTEAKSSELNL-- 285
Query: 710 GIQNPSFACACHFPSEPSFAASVPCVHTSPKAPTMA--VRSKLKPKHSSVQNGVVADKVS 537
P+ + S+PC H+S +A V + KPK+S V NG+V++K
Sbjct: 286 -------------PNPCNPVHSIPCAHSSQSETPIASGVPNLAKPKNSLVLNGLVSEKDG 332
Query: 536 IPN---TFHKSFTAVGNTHSLVSEEKFICSRKV-SGITKREYILRQKSIQLEKSYRTYGS 369
+ N T KSF+ G + S EEKF SRKV SG TKRE +LRQKS+ LEK+YRTYG
Sbjct: 333 LNNTSDTTDKSFSVTGTSQSAAPEEKFGLSRKVHSGGTKRESMLRQKSLHLEKNYRTYGC 392
Query: 368 RGASRAGKLGSFGGLVLDKKIKS 300
+G+SR KL G +LDKK+KS
Sbjct: 393 KGSSRTAKLSGLGSYMLDKKLKS 415
Score = 41.6 bits (96), Expect(2) = e-124
Identities = 28/78 (35%), Positives = 43/78 (55%), Gaps = 1/78 (1%)
Frame = -3
Query: 301 VADSTAISVRNATSKISKELTASLLQTSMNNDLLTTNDPPPS-AFSSEKVNHNISSPKTN 125
V+DST ++++NA+ KISK + + Q + N++L + S AF+ E VN S PKTN
Sbjct: 416 VSDSTGVNLKNASLKISKAMGVDVPQDNGNHNLSPNSGLSSSAAFNLETVNTIGSLPKTN 475
Query: 124 IISTITGASDQPSLPAAD 71
S + + P AD
Sbjct: 476 SPSALPPVNTPPIPSGAD 493
>ref|XP_002516824.1| conserved hypothetical protein [Ricinus communis]
gi|223543912|gb|EEF45438.1| conserved hypothetical
protein [Ricinus communis]
Length = 894
Score = 436 bits (1122), Expect(2) = e-124
Identities = 248/443 (55%), Positives = 304/443 (68%), Gaps = 7/443 (1%)
Frame = -2
Query: 1607 MASLVAKACNSG-SGQMMPVIAMQEKGSRNKRKFRADPPSPDTSKTLSLPLGDYSSYEFS 1431
MAS+VAKA +S S Q+ ++++QEKGSRNKRKFRAD P D K + P + S YEFS
Sbjct: 1 MASMVAKASSSSCSTQVSSLVSVQEKGSRNKRKFRADTPLGDPGKIIPSPQNECSGYEFS 60
Query: 1430 AEKLKSIQNHGHASGCDMCNVGHASGCDMCNVGREHSDSLKLDLGLSCAVGPYEAGTSKP 1251
AEK ++ HG +S CD+C V ++HS+ LKLDLGLS A+ E GTS+P
Sbjct: 61 AEKFEATPAHGPSSVCDLCGVN-----------QDHSEGLKLDLGLSSALSSSEVGTSQP 109
Query: 1250 KDEIVYAGALHDADWSDLTESQLEELVLDNLDAILKSAIKKLMDCGYSEELCTRAVVKSG 1071
++E+ + HDADWSDLTESQLEELVL NLDAI KSAIKK++ CGY+EE+ T+AV++SG
Sbjct: 110 REELESEES-HDADWSDLTESQLEELVLSNLDAIFKSAIKKIVACGYTEEVATKAVLRSG 168
Query: 1070 LCYGCKDIVSNIVDNALALLRNGQDLDISREHDFEDLLQMEKYILAELVCVIREVKPFFS 891
LCYGCKD VSNIVDN LA LRNGQ++D SR+H FEDL Q+EKYILAELVCV+REV+PFFS
Sbjct: 169 LCYGCKDTVSNIVDNTLAFLRNGQEIDPSRDHCFEDLQQLEKYILAELVCVLREVRPFFS 228
Query: 890 TGGAMWCLLVCDMNVSLACAMESDPFSNLIGDRAPYGSPKAQVQPQMKIETRSSENHLNV 711
TG AMWCLL+CDMNVS ACAM+ DP S GD G+ QPQ IE++SSE +
Sbjct: 229 TGDAMWCLLICDMNVSHACAMDGDPLSGFAGDGTSNGTSSTSNQPQ--IESKSSE----L 282
Query: 710 GIQNPSFACACHFPSEPSFAASVPCVHTSPKAPTMAVR--SKLKPKHSSVQNGVVADKVS 537
+ NP SEPS S +AP + R + KPK+S +G+V +K
Sbjct: 283 NLPNPC-------KSEPSVTCS------QSEAPNIMTRVPNISKPKNSVAVSGLVTEKDG 329
Query: 536 IPNTF---HKSFTAVGNTHSLVSEEKFICSRKV-SGITKREYILRQKSIQLEKSYRTYGS 369
+TF KSF+ G + S V EEK I SRKV S TKREYILRQKS+ LEK YRTYG
Sbjct: 330 SNSTFDSADKSFSVAGTSQSPVVEEKLIVSRKVHSNSTKREYILRQKSLHLEKGYRTYGP 389
Query: 368 RGASRAGKLGSFGGLVLDKKIKS 300
+G SRAGKL GGL+LDKK+KS
Sbjct: 390 KG-SRAGKLSGLGGLILDKKLKS 411
Score = 37.0 bits (84), Expect(2) = e-124
Identities = 21/82 (25%), Positives = 44/82 (53%), Gaps = 1/82 (1%)
Frame = -3
Query: 313 KK*RVADSTAISVRNATSKISKELTASLLQTSMNNDLLT-TNDPPPSAFSSEKVNHNISS 137
KK + +A++++NA+ ++SK + + Q + + +L + T P++F+ E +
Sbjct: 407 KKLKSVSESAVNIKNASLRLSKVMGVDVSQDNASQNLSSNTRSSSPASFNLETSGTTSAF 466
Query: 136 PKTNIISTITGASDQPSLPAAD 71
PKTN S + + P+L A +
Sbjct: 467 PKTNNQSALPVVTKPPALTAVN 488
>ref|XP_004159841.1| PREDICTED: LOW QUALITY PROTEIN: putative E3 ubiquitin-protein ligase
RF298-like [Cucumis sativus]
Length = 901
Score = 376 bits (965), Expect(2) = e-108
Identities = 220/443 (49%), Positives = 287/443 (64%), Gaps = 7/443 (1%)
Frame = -2
Query: 1607 MASLVAK-ACNSGSGQMMPVIAMQEKGSRNKRKFRADPPSPDTSKTLSLPLGDYSSYEFS 1431
MAS+VAK +C S S + +QEKGSRNKRK+RADPP D +K S SYEFS
Sbjct: 1 MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60
Query: 1430 AEKLKSIQNHGHASGCDMCNVGHASGCDMCNVGREHSDSLKLDLGLSCAVGPYEAGTSKP 1251
AEK + + +G +SGCD+C++ +E S LKLDLGLS G + G + P
Sbjct: 61 AEKFEISSS-----------MGQSSGCDLCSISQEFSAGLKLDLGLSNG-GSSDVGINWP 108
Query: 1250 KDEIVYAGALHDADWSDLTESQLEELVLDNLDAILKSAIKKLMDCGYSEELCTRAVVKSG 1071
+ E+ DADWSDLTE+QLEELVL NLD I K AIKK++ GY+EE+ +AV +SG
Sbjct: 109 RGELE-VDEDQDADWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSG 167
Query: 1070 LCYGCKDIVSNIVDNALALLRNGQDLDISREHDFEDLLQMEKYILAELVCVIREVKPFFS 891
+C+G KD VSN+VDN LA LR GQ++D SREH FEDL Q+EKYILAELVCV+RE++PFFS
Sbjct: 168 ICFGGKDTVSNVVDNTLAFLRRGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFS 227
Query: 890 TGGAMWCLLVCDMNVSLACAMESDPFSNLIGDRAPYGSPKAQVQPQMKIETRSSENHLNV 711
TG AMWCLL+ DM+V+LACAM+SDP + L+ D S + PQ+K E +SSE +L
Sbjct: 228 TGDAMWCLLISDMSVALACAMDSDPCNALVCDGTSNESSSNTI-PQLKAEVKSSEMNL-- 284
Query: 710 GIQNPSFACACHFPSEPSFAASVPCVHTS--PKAPTMAVRSKLKPKHSSVQNGVVADKVS 537
P + + C H S T+ V S KPK +G +++K
Sbjct: 285 -------------PKPVKPISPISCAHGSQYDGPATVGVPSISKPKDPLFSSGPLSEKEL 331
Query: 536 IPNTF---HKSFTAVGNTHSLVSEEKFICSRKV-SGITKREYILRQKSIQLEKSYRTYGS 369
+TF +SF+ N+ + VSEEK SRKV S ITKREY+LRQKS+ ++K++RTYG+
Sbjct: 332 QNSTFDVVEESFSVAVNSQTSVSEEKIESSRKVHSNITKREYMLRQKSLHVDKNFRTYGA 391
Query: 368 RGASRAGKLGSFGGLVLDKKIKS 300
+G+SRAGKL GGL+LDKK+KS
Sbjct: 392 KGSSRAGKLTGLGGLMLDKKLKS 414
Score = 45.1 bits (105), Expect(2) = e-108
Identities = 27/76 (35%), Positives = 45/76 (59%), Gaps = 1/76 (1%)
Frame = -3
Query: 301 VADSTAISVRNATSKISKELTASLLQTSMNNDLLTTNDPPPS-AFSSEKVNHNISSPKTN 125
V+ STA++ +NA+ KISK + + Q + +++L T + P S F+ E +N KTN
Sbjct: 415 VSGSTAVNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSPFSKTN 474
Query: 124 IISTITGASDQPSLPA 77
+ S++ S P+LPA
Sbjct: 475 LPSSMPAPSSPPALPA 490
>ref|XP_004134170.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298-like [Cucumis
sativus]
Length = 901
Score = 376 bits (965), Expect(2) = e-108
Identities = 220/443 (49%), Positives = 287/443 (64%), Gaps = 7/443 (1%)
Frame = -2
Query: 1607 MASLVAK-ACNSGSGQMMPVIAMQEKGSRNKRKFRADPPSPDTSKTLSLPLGDYSSYEFS 1431
MAS+VAK +C S S + +QEKGSRNKRK+RADPP D +K S SYEFS
Sbjct: 1 MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60
Query: 1430 AEKLKSIQNHGHASGCDMCNVGHASGCDMCNVGREHSDSLKLDLGLSCAVGPYEAGTSKP 1251
AEK + + +G +SGCD+C++ +E S LKLDLGLS G + G + P
Sbjct: 61 AEKFEISSS-----------MGQSSGCDLCSISQEFSAGLKLDLGLSNG-GSSDVGINWP 108
Query: 1250 KDEIVYAGALHDADWSDLTESQLEELVLDNLDAILKSAIKKLMDCGYSEELCTRAVVKSG 1071
+ E+ DADWSDLTE+QLEELVL NLD I K AIKK++ GY+EE+ +AV +SG
Sbjct: 109 RGELE-VDEDQDADWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSG 167
Query: 1070 LCYGCKDIVSNIVDNALALLRNGQDLDISREHDFEDLLQMEKYILAELVCVIREVKPFFS 891
+C+G KD VSN+VDN LA LR GQ++D SREH FEDL Q+EKYILAELVCV+RE++PFFS
Sbjct: 168 ICFGGKDTVSNVVDNTLAFLRRGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFS 227
Query: 890 TGGAMWCLLVCDMNVSLACAMESDPFSNLIGDRAPYGSPKAQVQPQMKIETRSSENHLNV 711
TG AMWCLL+ DM+V+LACAM+SDP + L+ D S + PQ+K E +SSE +L
Sbjct: 228 TGDAMWCLLISDMSVALACAMDSDPCNALVCDGTSNESSSNTI-PQLKAEVKSSEMNL-- 284
Query: 710 GIQNPSFACACHFPSEPSFAASVPCVHTS--PKAPTMAVRSKLKPKHSSVQNGVVADKVS 537
P + + C H S T+ V S KPK +G +++K
Sbjct: 285 -------------PKPVKPISPISCAHGSQYDGPATVGVPSISKPKDPLFSSGPLSEKEL 331
Query: 536 IPNTF---HKSFTAVGNTHSLVSEEKFICSRKV-SGITKREYILRQKSIQLEKSYRTYGS 369
+TF +SF+ N+ + VSEEK SRKV S ITKREY+LRQKS+ ++K++RTYG+
Sbjct: 332 QNSTFDVVEESFSVAVNSQTSVSEEKIESSRKVHSNITKREYMLRQKSLHVDKNFRTYGA 391
Query: 368 RGASRAGKLGSFGGLVLDKKIKS 300
+G+SRAGKL GGL+LDKK+KS
Sbjct: 392 KGSSRAGKLTGLGGLMLDKKLKS 414
Score = 45.1 bits (105), Expect(2) = e-108
Identities = 27/76 (35%), Positives = 45/76 (59%), Gaps = 1/76 (1%)
Frame = -3
Query: 301 VADSTAISVRNATSKISKELTASLLQTSMNNDLLTTNDPPPS-AFSSEKVNHNISSPKTN 125
V+ STA++ +NA+ KISK + + Q + +++L T + P S F+ E +N KTN
Sbjct: 415 VSGSTAVNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSPFSKTN 474
Query: 124 IISTITGASDQPSLPA 77
+ S++ S P+LPA
Sbjct: 475 LPSSMPAPSSPPALPA 490
>ref|XP_003546114.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298-like [Glycine
max]
Length = 855
Score = 396 bits (1017), Expect = e-107
Identities = 229/453 (50%), Positives = 286/453 (63%), Gaps = 17/453 (3%)
Frame = -2
Query: 1607 MASLVAKACNSGSGQMMPVIAMQEKGSRNKRKFRADPPSPDTSKTLSLPLGDYSSYEFSA 1428
MASLVA SGS QM P +++QEKGSRNKRKFRADPP + +K + LP + SYEFSA
Sbjct: 1 MASLVA----SGSSQMAPSVSVQEKGSRNKRKFRADPPLGEPNKIIPLPQHESLSYEFSA 56
Query: 1427 EKLKSIQNHGHASGCDMCNVGHASGCDMCNVGREHSDSLKLDLGLSCAVGPYEAGTSKPK 1248
EK + HG S ASG MC+V ++HSD+LKLDLGLS V + S+PK
Sbjct: 57 EKFEITPGHGQVS---------ASG--MCSVSQDHSDALKLDLGLSSPVASSDVRISQPK 105
Query: 1247 DEIVYAGALHDADWSDLTESQLEELVLDNLDAILKSAIKKLMDCGYSEELCTRAVVKSGL 1068
+E+ HDADWSDLTE+QLEELVL NLD I KSAIKK++ CGY E++ T+A+++SG+
Sbjct: 106 EELE-VDEFHDADWSDLTEAQLEELVLSNLDTIFKSAIKKIVACGYIEDVATKAILRSGI 164
Query: 1067 CYGCKDIVSNIVDNALALLRNGQDLDISREHDFEDLLQMEKYILAELVCVIREVKPFFST 888
CYGCKD VSN+VDN LA LRNGQ+++ SREH FEDL+Q+EKYILAELVCV+REV+P FST
Sbjct: 165 CYGCKDAVSNVVDNGLAFLRNGQEINPSREHYFEDLVQLEKYILAELVCVLREVRPLFST 224
Query: 887 GGAMWCLLVCDMNVSLACAMESDPFSNLIGDRAPYGSPKAQVQPQMKIETRSSENHLNVG 708
G AMW LL+CDMNVSLACAM+ DP S+L D G Q + Q K+ET+ E
Sbjct: 225 GDAMWRLLICDMNVSLACAMDGDPSSSLGSDGIADGCSSVQTESQSKLETKGPE------ 278
Query: 707 IQNPSFACACHFPSEPSFAASVPCVHTSPKAPTMAVRSKLKPKHSSVQNGVVADKV---- 540
S+P +P +V S +PK SSV+ DK
Sbjct: 279 -------------------LSLP-------SPCKSVSSGSQPKKSSVEGNTGLDKSKNSQ 312
Query: 539 ------------SIPNTFHKSFTAVGNTHSLVSEEKFICSRKV-SGITKREYILRQKSIQ 399
S ++ KS + G + S + EEK RKV S TKR+YILRQKS
Sbjct: 313 ILVGPSEKEAANSGRDSIDKSSSTSGTSQSPLVEEKCGNIRKVHSSSTKRDYILRQKSFH 372
Query: 398 LEKSYRTYGSRGASRAGKLGSFGGLVLDKKIKS 300
+EK YRTYGS+G+SR G+L GL+LDKK+KS
Sbjct: 373 MEKGYRTYGSKGSSRGGRLNGLNGLILDKKLKS 405