BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00004720
         (1049 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002530483.1| amidase, putative [Ricinus communis] gi|2235...   290   4e-76
ref|XP_003525187.1| PREDICTED: glutamyl-tRNA(Gln) amidotransfera...   283   5e-74
ref|XP_003530960.1| PREDICTED: glutamyl-tRNA(Gln) amidotransfera...   281   2e-73
ref|XP_002322105.1| amidase family protein [Populus trichocarpa]...   281   2e-73
ref|XP_002317872.1| amidase family protein [Populus trichocarpa]...   280   4e-73

>ref|XP_002530483.1| amidase, putative [Ricinus communis] gi|223529980|gb|EEF31906.1|
           amidase, putative [Ricinus communis]
          Length = 589

 Score =  290 bits (742), Expect = 4e-76
 Identities = 134/209 (64%), Positives = 176/209 (84%)
 Frame = -3

Query: 627 GKTVVDDMAFSISGECKHYDTPTNPAVPARIPXXXXXXXXXXXXANLVDFSLGIDVVGDV 448
           GKTVVD++A+SI+GE +HY TPTNPAVPAR+P            ANLVDFSLG+D VG V
Sbjct: 109 GKTVVDELAYSINGENEHYGTPTNPAVPARVPGGSSSGAAVAVAANLVDFSLGVDTVGGV 168

Query: 447 RIPAGYCGVLGFRPSHGAIPHVGIIPIASSLDTVGWFAKDPNILRQVGHVLLQLPFGVQR 268
           R+PAG+CG++GFRPS+GAIP+ GI+PI++SLDTVGWFA+DPN++R+VGH+LLQLPFGVQR
Sbjct: 169 RVPAGFCGIIGFRPSYGAIPNTGILPISASLDTVGWFARDPNVIRRVGHLLLQLPFGVQR 228

Query: 267 NPRNVMIADDCFELLKTPVDRITEVVIRSTEKLFGKQVLKHVNLGDYISSKVPRLQVLHS 88
           NP+ ++IADDCF+LLK PVDRI +VVI+STEK+FG+QVL+H  L D + S+V  L++LH 
Sbjct: 229 NPKQIIIADDCFQLLKIPVDRIAQVVIKSTEKIFGRQVLRHEILEDVLFSRVRSLKLLHD 288

Query: 87  NKANGEVKPSSLRSLAHMMQLLWRYEFKN 1
            K NGE++ SS+R LA++M+LL RYEF++
Sbjct: 289 QKTNGELRTSSIRLLANIMRLLHRYEFRH 317



 Score =  111 bits (278), Expect = 3e-22
 Identities = 56/92 (60%), Positives = 66/92 (71%)
 Frame = -2

Query: 1036 STAANLLVLVGLSIAGIFMMTKKLKKTIKPDFGAFVQRFXXXXXXXXXXXXXXXXLTSLT 857
            S  ANL VL+GL IAGI ++TKKLKK I+ DFGAFVQ+                 LT LT
Sbjct: 3    SNPANLWVLLGLGIAGILLVTKKLKKVIREDFGAFVQKLQLLPPPPPAPPKAPHPLTGLT 62

Query: 856  FAISDIFDVGGYVTGFGNPDWARTHEAASRTS 761
            FA+SD+FD+ GYVTGFG+PDWARTHEAAS+TS
Sbjct: 63   FAVSDVFDIEGYVTGFGHPDWARTHEAASQTS 94


>ref|XP_003525187.1| PREDICTED: glutamyl-tRNA(Gln) amidotransferase subunit A-like
           [Glycine max]
          Length = 587

 Score =  283 bits (724), Expect = 5e-74
 Identities = 131/206 (63%), Positives = 166/206 (80%)
 Frame = -3

Query: 621 TVVDDMAFSISGECKHYDTPTNPAVPARIPXXXXXXXXXXXXANLVDFSLGIDVVGDVRI 442
           TV+DD+A  I GE KH+ TPTNPAVPAR+P            AN VDF+LGID  G VR+
Sbjct: 108 TVLDDLALGIGGENKHFGTPTNPAVPARVPGGSSSGAAVAVAANFVDFALGIDTTGGVRV 167

Query: 441 PAGYCGVLGFRPSHGAIPHVGIIPIASSLDTVGWFAKDPNILRQVGHVLLQLPFGVQRNP 262
           PAG+CG+LGFRPSHGA+ H+GIIPI++SLDTVGWFAKDPNILR+VGH+LLQ PF +QR+P
Sbjct: 168 PAGFCGILGFRPSHGAVSHMGIIPISTSLDTVGWFAKDPNILRRVGHILLQAPFVMQRSP 227

Query: 261 RNVMIADDCFELLKTPVDRITEVVIRSTEKLFGKQVLKHVNLGDYISSKVPRLQVLHSNK 82
           R ++IADDCF+ +  P+DR ++VV+++TEKLFG+QVLKH+NLGDY+SS+VP L+     K
Sbjct: 228 RQIVIADDCFQHINVPLDRSSQVVVKATEKLFGRQVLKHINLGDYLSSRVPSLKGCSGQK 287

Query: 81  ANGEVKPSSLRSLAHMMQLLWRYEFK 4
            NGEVK SSL+ LAH+MQ L R+EF+
Sbjct: 288 PNGEVKASSLKLLAHIMQFLQRHEFR 313



 Score = 95.1 bits (235), Expect = 3e-17
 Identities = 45/91 (49%), Positives = 62/91 (68%)
 Frame = -2

Query: 1030 AANLLVLVGLSIAGIFMMTKKLKKTIKPDFGAFVQRFXXXXXXXXXXXXXXXXLTSLTFA 851
            A NLLVL+GL +AGI ++T+KL K+++ DFGAF+ +                 LTSLTFA
Sbjct: 2    AKNLLVLLGLGLAGIVLITRKLNKSVREDFGAFIHKLQLLPPPQPSPPKAPHPLTSLTFA 61

Query: 850  ISDIFDVGGYVTGFGNPDWARTHEAASRTSP 758
            +SD+F + G+V  FG+PDWARTHE +S T+P
Sbjct: 62   LSDLFHIHGHVPSFGHPDWARTHEPSSSTAP 92


>ref|XP_003530960.1| PREDICTED: glutamyl-tRNA(Gln) amidotransferase subunit A-like
           [Glycine max]
          Length = 591

 Score =  281 bits (719), Expect = 2e-73
 Identities = 130/206 (63%), Positives = 167/206 (81%)
 Frame = -3

Query: 621 TVVDDMAFSISGECKHYDTPTNPAVPARIPXXXXXXXXXXXXANLVDFSLGIDVVGDVRI 442
           TV+DD+A  I GE KHY TPTNPAVPAR+P            A+ VDF+LGID VG VR+
Sbjct: 112 TVLDDLALGIGGENKHYGTPTNPAVPARVPGGSSSGAAVAVAADFVDFALGIDTVGGVRV 171

Query: 441 PAGYCGVLGFRPSHGAIPHVGIIPIASSLDTVGWFAKDPNILRQVGHVLLQLPFGVQRNP 262
           PAG+CG+LGFRPSHGA+ H+GIIPI++SLDTVGWFAKDPNILR+VGH+LLQ PF +QR+P
Sbjct: 172 PAGFCGILGFRPSHGAVSHLGIIPISTSLDTVGWFAKDPNILRRVGHILLQAPFAMQRSP 231

Query: 261 RNVMIADDCFELLKTPVDRITEVVIRSTEKLFGKQVLKHVNLGDYISSKVPRLQVLHSNK 82
           R ++IADDCF+ +  P+DR ++VV+++TEKLFG+QVLKH+NLGDY+SS+VP L+     K
Sbjct: 232 RQIVIADDCFQHINVPLDRSSQVVVKTTEKLFGRQVLKHINLGDYLSSRVPSLKGCSGQK 291

Query: 81  ANGEVKPSSLRSLAHMMQLLWRYEFK 4
            NGEVK S+L+ LA++MQ L R+EF+
Sbjct: 292 TNGEVKASALKLLANIMQSLQRHEFR 317



 Score = 99.0 bits (245), Expect = 2e-18
 Identities = 46/95 (48%), Positives = 68/95 (71%)
 Frame = -2

Query: 1042 SSSTAANLLVLVGLSIAGIFMMTKKLKKTIKPDFGAFVQRFXXXXXXXXXXXXXXXXLTS 863
            +S +A NLLVL+GL +AGI ++T+KLKK+++ D GAF+++                 LT+
Sbjct: 2    TSQSAKNLLVLLGLGLAGIVLITRKLKKSVRKDLGAFIEKLQLLPPPQPAPPKAPHPLTA 61

Query: 862  LTFAISDIFDVGGYVTGFGNPDWARTHEAASRTSP 758
            LTFA+SD+FD+ G+V+ FG+P+WARTHE AS T+P
Sbjct: 62   LTFALSDLFDIEGHVSTFGHPEWARTHEPASSTAP 96


>ref|XP_002322105.1| amidase family protein [Populus trichocarpa]
           gi|222869101|gb|EEF06232.1| amidase family protein
           [Populus trichocarpa]
          Length = 592

 Score =  281 bits (719), Expect = 2e-73
 Identities = 132/209 (63%), Positives = 169/209 (80%)
 Frame = -3

Query: 627 GKTVVDDMAFSISGECKHYDTPTNPAVPARIPXXXXXXXXXXXXANLVDFSLGIDVVGDV 448
           GKTV+D++A+SI+GE KHY TPTNP  PAR+P            ANLVDFSLG+D VG V
Sbjct: 109 GKTVIDELAYSITGENKHYGTPTNPVEPARVPGGSSSGAAVAVAANLVDFSLGVDTVGGV 168

Query: 447 RIPAGYCGVLGFRPSHGAIPHVGIIPIASSLDTVGWFAKDPNILRQVGHVLLQLPFGVQR 268
           R+PAG+CG++GFRPS+GAI  +G++P++ SLDTVGWFAKDPNILR+VGHVLLQ  FG QR
Sbjct: 169 RVPAGFCGIIGFRPSYGAISKIGVLPVSVSLDTVGWFAKDPNILRRVGHVLLQSAFGGQR 228

Query: 267 NPRNVMIADDCFELLKTPVDRITEVVIRSTEKLFGKQVLKHVNLGDYISSKVPRLQVLHS 88
           +PR +++ADDCF+LLK PVDRI +VV++STEK FG+Q+LKH  L  Y++SKVP L+  H+
Sbjct: 229 SPRQIIMADDCFQLLKIPVDRIAQVVVKSTEKHFGRQLLKHEILDVYLNSKVPSLKEFHN 288

Query: 87  NKANGEVKPSSLRSLAHMMQLLWRYEFKN 1
            K NGEVK SS+R LA++MQLL RYEF++
Sbjct: 289 KKTNGEVKTSSIRLLANVMQLLHRYEFRS 317



 Score =  108 bits (270), Expect = 2e-21
 Identities = 52/93 (55%), Positives = 65/93 (69%)
 Frame = -2

Query: 1039 SSTAANLLVLVGLSIAGIFMMTKKLKKTIKPDFGAFVQRFXXXXXXXXXXXXXXXXLTSL 860
            +S  ANL VL+GL +AGIF+ TKK KK ++ DFGAF+ +                 LT L
Sbjct: 2    ASNPANLWVLLGLGLAGIFLFTKKFKKVLREDFGAFIHKLQLLPPPQPAPPKAPHPLTGL 61

Query: 859  TFAISDIFDVGGYVTGFGNPDWARTHEAASRTS 761
            TFA+SD+FD+ GYVTGFG+PDWA+THEAASRTS
Sbjct: 62   TFAVSDLFDIEGYVTGFGHPDWAKTHEAASRTS 94


>ref|XP_002317872.1| amidase family protein [Populus trichocarpa]
           gi|222858545|gb|EEE96092.1| amidase family protein
           [Populus trichocarpa]
          Length = 593

 Score =  280 bits (716), Expect = 4e-73
 Identities = 132/209 (63%), Positives = 167/209 (79%)
 Frame = -3

Query: 627 GKTVVDDMAFSISGECKHYDTPTNPAVPARIPXXXXXXXXXXXXANLVDFSLGIDVVGDV 448
           GKTV+D++A+SI GE KHY TP NP VPAR+P            ANLVDFSLG+D VG V
Sbjct: 110 GKTVIDELAYSIHGENKHYGTPINPVVPARVPGGSCSGAAVAVAANLVDFSLGVDTVGGV 169

Query: 447 RIPAGYCGVLGFRPSHGAIPHVGIIPIASSLDTVGWFAKDPNILRQVGHVLLQLPFGVQR 268
           R+PAG+CGV+GFRPS+GAI   G++P+++SLDTVGWFAKDPNILR+VGHVLLQ  FG QR
Sbjct: 170 RVPAGFCGVIGFRPSYGAISKTGVLPVSASLDTVGWFAKDPNILRRVGHVLLQPAFGGQR 229

Query: 267 NPRNVMIADDCFELLKTPVDRITEVVIRSTEKLFGKQVLKHVNLGDYISSKVPRLQVLHS 88
           +PR +++A+DCF+LLK PVDR+ +VV+ STEK FG+QVLKH  L  Y++SKVP L+  H+
Sbjct: 230 SPRQIIMAEDCFQLLKIPVDRVAQVVVNSTEKHFGRQVLKHEILDVYLNSKVPSLKEFHN 289

Query: 87  NKANGEVKPSSLRSLAHMMQLLWRYEFKN 1
            K NG+VK SS+R LAH+MQLL RYEF++
Sbjct: 290 KKKNGDVKTSSIRLLAHVMQLLHRYEFRS 318



 Score =  100 bits (250), Expect = 5e-19
 Identities = 49/94 (52%), Positives = 69/94 (73%), Gaps = 1/94 (1%)
 Frame = -2

Query: 1039 SSTAANLLVLVGLSIAGIFMMTKKLKKTI-KPDFGAFVQRFXXXXXXXXXXXXXXXXLTS 863
            +S +ANL VL+GL +AGI +MTKK KK+I + DFGAF+++                 LT+
Sbjct: 2    ASNSANLWVLLGLGLAGILLMTKKFKKSIIRQDFGAFIEKLLLVPPPQPPPPKAPHPLTA 61

Query: 862  LTFAISDIFDVGGYVTGFGNPDWARTHEAASRTS 761
            LTFA+SD+F++ GYV+GFG+P+WA+TH+AASRTS
Sbjct: 62   LTFAVSDLFEIEGYVSGFGHPEWAKTHQAASRTS 95