BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00004689
         (1739 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002269731.1| PREDICTED: E3 ubiquitin-protein ligase RNF16...   533   e-149
ref|XP_002325986.1| predicted protein [Populus trichocarpa] gi|2...   520   e-145
ref|XP_002333929.1| predicted protein [Populus trichocarpa] gi|2...   508   e-141
emb|CBI18269.3| unnamed protein product [Vitis vinifera]              496   e-138
ref|XP_004171019.1| PREDICTED: E3 ubiquitin-protein ligase RNF16...   490   e-136

>ref|XP_002269731.1| PREDICTED: E3 ubiquitin-protein ligase RNF167-like [Vitis vinifera]
          Length = 446

 Score =  533 bits (1373), Expect = e-149
 Identities = 295/443 (66%), Positives = 329/443 (74%), Gaps = 11/443 (2%)
 Frame = +2

Query: 206  VFILC-FLNLISTRVLANVVLMTNNATLSFEDIDANFAPSVKGSGESGTLYKADPLDACS 382
            V + C FLNL+++   ANVVL+ NN TLSF+DI ANFA  VKGSGE G LY A+PLDACS
Sbjct: 7    VLLFCAFLNLMASVAFANVVLIGNNVTLSFDDIQANFAKPVKGSGECGILYVAEPLDACS 66

Query: 383  PLKNIVPALKDGDLAPFVLIVRGGCSFEDKVRRAQIAGFKAAIVYDNEVGD-LVAMAGNS 559
            PL N V  + +G  +PFVLIVRGGC FEDKVR AQ AGF AAIVYDNE G  LVAMAGNS
Sbjct: 67   PLTNAVEKV-EGASSPFVLIVRGGCGFEDKVRNAQKAGFAAAIVYDNEDGGVLVAMAGNS 125

Query: 560  AGIKIHAVFISKASGELLTDYVGATDLELWIIPSFENSAWSIMAISFISLLAMSAVLATC 739
            AGIKIHAVF+SKASG +L  Y G+ D+ELWIIPSFENSAWSIMAISFISLLAMSAVLATC
Sbjct: 126  AGIKIHAVFVSKASGMVLQTYAGSPDMELWIIPSFENSAWSIMAISFISLLAMSAVLATC 185

Query: 740  FFXXXXXXXXXXXXXXXXXXFHGMSSQLVKAMPSLIFTAVLDDNCTSSTCAICIEDYTVG 919
            FF                  FHGMSS+LVKAMPSLIFT VL+DNCTS TCAIC+EDY VG
Sbjct: 186  FFVRRHRIRRQRPRVSRVREFHGMSSRLVKAMPSLIFTTVLEDNCTSRTCAICLEDYNVG 245

Query: 920  EKLRVLPCRHKFHAACVDAWLTTWRTFCPVCKRDARTSTGEPPASERTP-----XXXXXX 1084
            EKLR+LPCRHKFHA CVD+WLT+WRTFCPVCKRDARTSTG+PP SE TP           
Sbjct: 246  EKLRILPCRHKFHAFCVDSWLTSWRTFCPVCKRDARTSTGDPP-SESTPLLSSSPGSLAP 304

Query: 1085 XXXXXXXXXXXXXXPAMQIGSSRS-NRFASISRVESYGSSFY-QQSLPSYRQSPRLGVSQ 1258
                           A+ I  S S +   S+SR  S  ++ Y QQSL SYRQS  L VS+
Sbjct: 305  SSLVSSVRSSLASSSAIHIAPSPSPSHSLSVSRNNSLSATPYIQQSLQSYRQS-SLSVSR 363

Query: 1259 SSLDLRNASSQRSIPPHLASSNSLGYPSLSPLNSRYMSPYIPSPGNAS-SYIGSAQQ-PH 1432
            SSLDLRNASSQRS   +L S +S+GYPSLSPLNSRY+SPYIPSP NAS SY+GS+ + PH
Sbjct: 364  SSLDLRNASSQRSHASYLVSPHSMGYPSLSPLNSRYLSPYIPSPSNASLSYVGSSSRPPH 423

Query: 1433 PLHLSESAASFSPYASAQSLPGC 1501
            PLH SESA SFSPY SAQSLP C
Sbjct: 424  PLHYSESATSFSPYTSAQSLPEC 446


>ref|XP_002325986.1| predicted protein [Populus trichocarpa] gi|222862861|gb|EEF00368.1|
            predicted protein [Populus trichocarpa]
          Length = 437

 Score =  520 bits (1340), Expect = e-145
 Identities = 280/438 (63%), Positives = 325/438 (74%), Gaps = 8/438 (1%)
 Frame = +2

Query: 212  ILCFLNLISTRVLA--NVVLMTNNATLSFEDIDANFAPSVKGSGESGTLYKADPLDACSP 385
            ++  L+L+S  ++A  NV+L+ NN T++F+DI+ANFAP++KGSGE G LY A+P+DACS 
Sbjct: 5    VVLLLSLLSCCLVASGNVLLIGNNVTMAFDDIEANFAPAIKGSGECGVLYLAEPIDACSD 64

Query: 386  LKNIVPALKDGDLA-PFVLIVRGGCSFEDKVRRAQIAGFKAAIVYDNEVGDLVAMAGNSA 562
            L N   A K  + + PFVLI+R GCSFEDKVRRAQ AG+KAAI+YDNE G LVAMAGNSA
Sbjct: 65   LTN--QAEKGSNCSSPFVLIIREGCSFEDKVRRAQKAGYKAAIIYDNEEGILVAMAGNSA 122

Query: 563  GIKIHAVFISKASGELLTDYVGATDLELWIIPSFENSAWSIMAISFISLLAMSAVLATCF 742
            G+ I AVF+SK SGE L  Y G TDLELWIIPSFENSAWSIMAISFISLLAMSAVLATCF
Sbjct: 123  GVTIPAVFVSKTSGETLKKYAGLTDLELWIIPSFENSAWSIMAISFISLLAMSAVLATCF 182

Query: 743  FXXXXXXXXXXXXXXXXXXFHGMSSQLVKAMPSLIFTAVLDDNCTSSTCAICIEDYTVGE 922
            F                  FHGMSS+LVKAMPSL FT+ L+DNCTS+TCAIC+EDYTVGE
Sbjct: 183  FVRRHRIRRERPRSSRVREFHGMSSRLVKAMPSLTFTSALEDNCTSTTCAICLEDYTVGE 242

Query: 923  KLRVLPCRHKFHAACVDAWLTTWRTFCPVCKRDARTSTGEPPASERTP---XXXXXXXXX 1093
            KLR+LPCRHKFHA CVD+WLTTWRTFCPVCKRDARTSTGEPPA+E TP            
Sbjct: 243  KLRILPCRHKFHAFCVDSWLTTWRTFCPVCKRDARTSTGEPPATESTPLLSSNPSSLASS 302

Query: 1094 XXXXXXXXXXXPAMQIGSSRSNRFASISRVESYGSSFY-QQSLPSYRQSPRLGVSQSSLD 1270
                        A+QI  SR+    S+S + S  S+ Y QQSL SYR SP + +SQSS D
Sbjct: 303  SMLSSFRSGTSTAIQITPSRT---PSVSYIPSLSSTPYAQQSLRSYRHSPSITLSQSSAD 359

Query: 1271 LRNASSQRSIPPHLASSNSLGYPSLSPLNSRYMSPYIPSPGNAS-SYIGSAQQPHPLHLS 1447
            LRN SS RS    L S +SLGYPS+SPLN+RYMS YIPSP NAS S + S++QP PLH S
Sbjct: 360  LRNMSSLRSRASFLVSPHSLGYPSISPLNTRYMSAYIPSPSNASPSLVSSSRQPRPLHCS 419

Query: 1448 ESAASFSPYASAQSLPGC 1501
            ESAASFSP+ASA SLP C
Sbjct: 420  ESAASFSPFASAHSLPDC 437


>ref|XP_002333929.1| predicted protein [Populus trichocarpa] gi|222839169|gb|EEE77520.1|
            predicted protein [Populus trichocarpa]
          Length = 461

 Score =  508 bits (1309), Expect = e-141
 Identities = 276/461 (59%), Positives = 323/461 (70%), Gaps = 31/461 (6%)
 Frame = +2

Query: 212  ILCFLNLISTRVLA--NVVLMTNNATLSFEDIDANFAPSVKGSGESGTLYKADPLDACSP 385
            ++  L+L+S  ++A  NVVL+ NN T++F DI+ANFAP++KGSGE G L  A+P+DAC+ 
Sbjct: 5    VVLLLSLLSCCLVASANVVLIGNNVTMAFNDIEANFAPAIKGSGECGVLSLAEPIDACTD 64

Query: 386  LKNIVPALKDGDLAPFVLIVRGGCSFEDKVRRAQIAGFKAAIVYDNEVGDLVA------- 544
            L N      +   +P+VLI+RGGCSFE KVRRAQ AGFKAAIV+DNE G LVA       
Sbjct: 65   LTNKAEKGLNSS-SPYVLIIRGGCSFEHKVRRAQKAGFKAAIVFDNEEGVLVASKTFSYV 123

Query: 545  --MAGNSAGIKIHAVFISKASGELLTDYVGATDLELWIIPSFENSAWSIMAISFISLLAM 718
              +AGNS G+KIHAVF+SK SGE LT Y G T LELW+IPSFENSAWSIMAISFISLLAM
Sbjct: 124  KIVAGNSVGVKIHAVFVSKKSGETLTKYAGLTGLELWLIPSFENSAWSIMAISFISLLAM 183

Query: 719  SAVLATCFFXXXXXXXXXXXXXXXXXXFHGMSSQLVKAMPSLIFTAVLDDNCTSSTCAIC 898
            SAVLATCFF                  FHGMSS+LVKAMPSL FT+VL+DNCTS+TCAIC
Sbjct: 184  SAVLATCFFIRRHRIRREHSHSSRVREFHGMSSRLVKAMPSLTFTSVLEDNCTSTTCAIC 243

Query: 899  IEDYTVGEKLRVLPCRHKFHAACVDAWLTTWRTFCPVCKRDARTSTGEPPASERTP---X 1069
            +EDYTVGEKLR+LPCRHKFHA CVD+WLTTWRTFCPVCKRDARTSTG+PPASE TP    
Sbjct: 244  LEDYTVGEKLRILPCRHKFHAFCVDSWLTTWRTFCPVCKRDARTSTGDPPASESTPLLSS 303

Query: 1070 XXXXXXXXXXXXXXXXXXXPAMQIGSSRSNRFASISRVESYGSSFY-QQSLPSYRQSPRL 1246
                                A+QI SSR+    S+S + S  S+ Y QQ L SYR S  +
Sbjct: 304  NPSSFASSSMLSSFRSGTSTAIQIASSRT---PSVSHISSLSSTPYVQQPLRSYRHSTSI 360

Query: 1247 GVSQSSLDLRNASSQRSIPPHLASSNSLGYPSLSPLNSRYMSPYIPSPGNA--------- 1399
             +S SS DLR+ SSQRS+  HL S +SLGYPS+SPLN+RYMS YIPSP NA         
Sbjct: 361  SISHSSADLRHMSSQRSLTSHLVSPHSLGYPSISPLNTRYMSAYIPSPSNASPSLVSSSH 420

Query: 1400 -------SSYIGSAQQPHPLHLSESAASFSPYASAQSLPGC 1501
                   S  + S+ QP PLH SESAASFS +ASAQSLPGC
Sbjct: 421  QPRPLYCSESVSSSHQPRPLHCSESAASFSSFASAQSLPGC 461


>emb|CBI18269.3| unnamed protein product [Vitis vinifera]
          Length = 424

 Score =  496 bits (1277), Expect = e-138
 Identities = 273/418 (65%), Positives = 305/418 (72%), Gaps = 3/418 (0%)
 Frame = +2

Query: 251  ANVVLMTNNATLSFEDIDANFAPSVKGSGESGTLYKADPLDACSPLKNIVPALKDGDLAP 430
            ANVVL+ NN TLSF+DI ANFA  VKGSGE G LY A+PLDACSPL N V  + +G  +P
Sbjct: 7    ANVVLIGNNVTLSFDDIQANFAKPVKGSGECGILYVAEPLDACSPLTNAVEKV-EGASSP 65

Query: 431  FVLIVRGGCSFEDKVRRAQIAGFKAAIVYDNEVGD-LVAMAGNSAGIKIHAVFISKASGE 607
            FVLIVRGGC FEDKVR AQ AGF AAIVYDNE G  LVAMAGNSAGIKIHAVF+SKASG 
Sbjct: 66   FVLIVRGGCGFEDKVRNAQKAGFAAAIVYDNEDGGVLVAMAGNSAGIKIHAVFVSKASGM 125

Query: 608  LLTDYVGATDLELWIIPSFENSAWSIMAISFISLLAMSAVLATCFFXXXXXXXXXXXXXX 787
            +L  Y G+ D+ELWIIPSFENSAWSIMAISFISLLAMSAVLATCFF              
Sbjct: 126  VLQTYAGSPDMELWIIPSFENSAWSIMAISFISLLAMSAVLATCFFVRRHRIRRQRPRVS 185

Query: 788  XXXXFHGMSSQLVKAMPSLIFTAVLDDNCTSSTCAICIEDYTVGEKLRVLPCRHKFHAAC 967
                FHGMSS+LVKAMPSLIFT VL+DNCTS TCAIC+EDY VGEKLR+LPCRHKFHA C
Sbjct: 186  RVREFHGMSSRLVKAMPSLIFTTVLEDNCTSRTCAICLEDYNVGEKLRILPCRHKFHAFC 245

Query: 968  VDAWLTTWRTFCPVCKRDARTSTGEPPASERTPXXXXXXXXXXXXXXXXXXXXPAMQIGS 1147
            VD+WLT+WRTFCPVCKRDARTSTG+PP SE TP                    P+  + S
Sbjct: 246  VDSWLTSWRTFCPVCKRDARTSTGDPP-SESTP----------LLSSSPGSLAPSSLVSS 294

Query: 1148 SRSNRFASISRVESYGSSFYQQSLPSYRQSPRLGVSQSSLDLRNASSQRSIPPHLASSNS 1327
             RS+  AS S +    S     SL   R +    +S +    +NASSQRS   +L S +S
Sbjct: 295  VRSS-LASSSAIHIAPSPSPSHSLSVSRNN---SLSATPYIQQNASSQRSHASYLVSPHS 350

Query: 1328 LGYPSLSPLNSRYMSPYIPSPGNAS-SYIGSAQQ-PHPLHLSESAASFSPYASAQSLP 1495
            +GYPSLSPLNSRY+SPYIPSP NAS SY+GS+ + PHPLH SESA SFSPY SAQSLP
Sbjct: 351  MGYPSLSPLNSRYLSPYIPSPSNASLSYVGSSSRPPHPLHYSESATSFSPYTSAQSLP 408


>ref|XP_004171019.1| PREDICTED: E3 ubiquitin-protein ligase RNF167-like [Cucumis sativus]
          Length = 463

 Score =  490 bits (1261), Expect = e-136
 Identities = 265/447 (59%), Positives = 309/447 (69%), Gaps = 17/447 (3%)
 Frame = +2

Query: 212  ILCFLNLISTRVLANVVLMTNNATLSFEDIDANFAPSVKGSGESGTLYKADPLDACSPLK 391
            +   L ++S+   ANVVL+  N TLSF+ I+ANFAPS++ SGE G LY A PLDACS L+
Sbjct: 20   LFSLLLVLSSGSAANVVLIGANVTLSFDSIEANFAPSIESSGEPGVLYLAKPLDACSTLE 79

Query: 392  N--IVPALKDGDLAPFVLIVRGGCSFEDKVRRAQIAGFKAAIVYDNEVGDLVAMAGNSAG 565
            N  +VP       +PF LIVRGGCSFEDKVRRAQ+AGFKAAI+YDNE G L+AMAG+SAG
Sbjct: 80   NKVVVPV---NTSSPFALIVRGGCSFEDKVRRAQVAGFKAAIIYDNEDGGLIAMAGSSAG 136

Query: 566  IKIHAVFISKASGELLTDYVGATDLELWIIPSFENSAWSIMAISFISLLAMSAVLATCFF 745
            I+IHAVF++K SGE L  Y G  ++E+WI+PSFENSAWSIMAISFISLLAMSAVLATCFF
Sbjct: 137  IRIHAVFVTKTSGETLKKYAGLANVEIWIVPSFENSAWSIMAISFISLLAMSAVLATCFF 196

Query: 746  XXXXXXXXXXXXXXXXXXFHGMSSQLVKAMPSLIFTAVLDDNCTSSTCAICIEDYTVGEK 925
                              F GMS ++VKAMPSLIFT  L+DNCTS TCAIC+EDYT GEK
Sbjct: 197  VRRHRIRRGRPRSSHTQEFRGMSRRVVKAMPSLIFTTALEDNCTSMTCAICLEDYTPGEK 256

Query: 926  LRVLPCRHKFHAACVDAWLTTWRTFCPVCKRDARTSTGEPPASERTP---XXXXXXXXXX 1096
            LR+LPCRHKFHA CVD+WLT WRTFCPVCKRDARTSTG+PP S  TP             
Sbjct: 257  LRILPCRHKFHALCVDSWLTAWRTFCPVCKRDARTSTGDPPPSASTPLLSSAPSSVASSS 316

Query: 1097 XXXXXXXXXXPAMQIGSSRSNRFASISRVESYG----------SSFYQQSLPSYRQSPRL 1246
                      P+  I  S + R  S+SR  S            SS++Q    SY QSP L
Sbjct: 317  ILSSSRSSLPPSSAIQISPALRSPSVSRNHSLSSTPYLHQSLISSYHQSPRSSYHQSPSL 376

Query: 1247 GVSQSSLDLRNASSQRSIPPHLASSNSLGYPSLSPLNSRYMSPYIPSPGNAS-SYIGSAQ 1423
             VS+SS++LRNASSQRS   HL S  S  YPS+SPL+SRYM P+IPSP NAS S IGS+ 
Sbjct: 377  SVSRSSVELRNASSQRSYTSHLISPYSSCYPSVSPLHSRYMIPHIPSPSNASPSIIGSSS 436

Query: 1424 QPH-PLHLSESAASFSPYASAQSLPGC 1501
              H PLH S SAAS SP+AS +SLP C
Sbjct: 437  HQHNPLHCSGSAASLSPFASTRSLPEC 463